CN110301430A - A kind of no animal originality additive sheep sperm efficient cryogenic preservation Dilution for powder liquid - Google Patents
A kind of no animal originality additive sheep sperm efficient cryogenic preservation Dilution for powder liquid Download PDFInfo
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- CN110301430A CN110301430A CN201810324076.8A CN201810324076A CN110301430A CN 110301430 A CN110301430 A CN 110301430A CN 201810324076 A CN201810324076 A CN 201810324076A CN 110301430 A CN110301430 A CN 110301430A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- Health & Medical Sciences (AREA)
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- Physiology (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The present invention now disclose it is a kind of by phosphatidyl choline and low-density lipoprotein substitution yolk without animal originality additive sheep sperm efficient cryogenic save Dilution for powder liquid, it is by lower distilled water 90-150mL, fructose 0.5-3g, trihydroxy aminomethane 1-5g, citric acid 1-3g, glutathione 30-100mg, bovine serum albumin(BSA) 20-150mg, atriphos 10-100mg, ten thousand unit streptomysin 5-40 of vitamin C 200-500mg penicillin 0-80, ten thousand unit, phosphatidyl choline 0.5-8g, low-density lipoprotein 0.5-8g is made by being dried, the Dilution for powder liquid has good effect to sheep sperm cryo-conservation, and production method is simple, production cost is low, it being capable of long-term preservation, it is easy to promote and utilize.
Description
Technical field
The present invention relates to a kind of non-animal derived property adding ingredients by phosphatidyl choline and low-density lipoprotein substitution yolk
Sheep artificial insemination efficient cryogenic save Dilution for powder liquid.
Technical background
Currently, China introduces many good sheep varieties from foreign countries, it is miscellaneous using fine quality and local sheep progress
Improvement is handed over, the production performance of local varieties is enhanced.In order to give full play to the reproductive potential of excellent sire, drove number is expanded rapidly
Amount, carrying out artificial insemination is method the most cost-effective.However sperm time-to-live under field conditions (factors) that sire is projected
Very short, needing being capable of long-term preservation and dilution liquid product easy to carry.Existing dilution yolk multi-purpose greatly is survived as sperm
Nutrition and energy matter, be possible in yolk carry preservation of some pathogenic factors to influence sperm, may be deposited in yolk
Pathogenic microorganism the reproductive organs of dam can be caused infringement influence dam fertility.Non-animal derived property in dilution
Adding ingredient is main trend, and to the needs that diluent ingredient is determined, yolk is as animality because of different cultivars, Different Nutrition
Level causes different to yolk ingredient itself, ultimately causes diluent ingredient and does not know, dilution effect is unstable；Animal sources
Property adding ingredient yolk can also cause to influence the sperm even potential heredity of ovum, this allows for the quality of dilution not
It can be guaranteed, influence follow-up test, production.Existing aqueous diluent all requirement conditions during production, transport, preservation
It is higher, it is easy pollution, dilution storage time is shorter, and because of difference large-scale production difficult to realize between yolk, constrains me
The development of state's sheep husbandry.
Summary of the invention
Aiming at the problems and shortcomings existing in the prior art, the purpose of the present invention is to provide a kind of non-animal derived property to add
The non-animal derived property Dilution for powder liquid that ingredient, preservation effect are good, production cost is low, the simple sheep efficient cryogenic of method saves.
Realizing the technical solution of above-mentioned purpose is: a kind of to save non-animal derived property addition for sheep sperm efficient cryogenic
Dilution for powder liquid, it is mainly made of following substance in proportion:
Preparation method of the present invention for sheep sperm cryo-conservation Dilution for powder liquid is by phosphatidyl choline and low-density
Lipoprotein mixes after drying process low temperature drying with other raw materials, and it is spare that low temperature crosses 80 meshes.
There are two types of use forms for sheep sperm cryo-conservation dilution by the present invention, first is that handling without drying process
Direct mixing low temp saves sheep sperm, and another form is formed into the use of dilution pulvis.
The present invention is basic liquid with TRIS, meta-alkalescence and has stronger buffer capacity to be conducive to improve sperm motility, phosphatide
Phatidylcholine and low-density lipoprotein substitution yolk save for a long time for sperm low temperature and provide protection, improve the stability and work of sperm
Power, be added to after ATP, Vc, BSA ingredient as sheep sperm cryo-conservation dilution can obtain it is ideal and stable as a result,
And it is easy to preparation and normalizing operation, it is not easy to pollute.
Specific embodiment
The specific preparation embodiment and product effect example provided below in conjunction with invention is of the invention to further illustrate
Beneficial effect.
It is prepared by the dilution of the sheep sperm low-temperature preservation of the present invention of embodiment 1
Distilled water 100mL and fructose 2g, trihydroxy aminomethane 5g, citric acid 2g, glutathione 50mg, ox blood is pure
Protein 10 0mg, atriphos 50mg, vitamin C 300mg, 300,000 unit streptomysin of penicillin, 200,000 unit, phosphatidyl gallbladder
Alkali 4g, low-density lipoprotein 4g are uniformly mixed to obtain the final product.
The preparation of the sheep sperm low-temperature preservation dilution pulvis of the present invention of embodiment 2
By fructose 2g, trihydroxy aminomethane 5g, citric acid 2g, glutathione 50mg, bovine serum albumin(BSA) 100mg, three
Adenosine phosphate 50mg, vitamin C 300mg, 300,000 unit streptomysin of penicillin, 200,000 unit and the phosphorus by frozen drying
It is spare to cross 80 meshes for low temperature after phosphatidylcholine 4g, low-density lipoprotein 4g are mixed in proportion.
The liquefaction of 3 dilution pulvis of embodiment
By Dilution for powder liquid and distilled water according to every 0.185g pulvis add distilled water be settled to 1mL ratio dissolve after mix
It can obtain.4 sperm cryo-conservation of embodiment
The sperm being collected is sent to laboratory, observes and measure sperm motility, density, selects the sperm of motility rate >=0.8,
It determines extension rate, is cooled to semen dilution to predetermined extension rate refrigerator water-bath 1.5h with complete Dilution for powder liquid is dissolved
0 DEG C, 0 DEG C of environment saves 1-10d, and when use thaws 20s in 35 DEG C of water-baths, microscopy, and 0.5 or more motility rate is used for semen deposition.
5 Dilution for powder liquid preservation of embodiment
It will be sealed by Dilution for powder liquid made of drying process and be saved backup at drying, the place of being protected from light
Influence of the 1 Different hypothermia preservation diluent of test example to sperm quality
Influence of the 1 Different hypothermia preservation diluent of table to sperm quality
Table 1 is the results show that apply TRIS basal liquid preservation effect not ideal enough sheep, using the dilution of TRIS patent yolk
Liquid has certain preservation effect as sheep sperm cryo-conservation, is made with the Dilution for powder liquid of the present invention that TRIS is basic liquid improvement
It can be obtained for sheep cryo-conservation dilution ideal and stable as a result, and in sperm motility rate, DNA percentage of head rice, conception rate etc.
Each index with yolk diluent no significant difference.
The influence to sperm motility rate is kept in dark place in 2 Dilution for powder liquid of test example
Cryopreserving sperm 9d sperm motility rate situation after different time is kept in dark place in 2 Dilution for powder liquid of table
Table 2 the result shows that, Dilution for powder liquid of the present invention be protected from light, after kept dry 90d with 0d when no significant difference, it is dry
Cryo-conservation sheep sperm still has the effect of more satisfactory and stable after preservation 90d.
Claims (1)
1. a kind of no animal originality additive sheep sperm efficient cryogenic saves Dilution for powder liquid, it is characterised in that it is by following
Substance is made in proportion:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810324076.8A CN110301430A (en) | 2018-03-27 | 2018-03-27 | A kind of no animal originality additive sheep sperm efficient cryogenic preservation Dilution for powder liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810324076.8A CN110301430A (en) | 2018-03-27 | 2018-03-27 | A kind of no animal originality additive sheep sperm efficient cryogenic preservation Dilution for powder liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110301430A true CN110301430A (en) | 2019-10-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810324076.8A Pending CN110301430A (en) | 2018-03-27 | 2018-03-27 | A kind of no animal originality additive sheep sperm efficient cryogenic preservation Dilution for powder liquid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115119830A (en) * | 2022-08-02 | 2022-09-30 | 塔里木大学 | Preparation and use method of cow semen low-temperature preservation diluent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101084926A (en) * | 2007-07-02 | 2007-12-12 | 高庆华 | Diluent for preserving frozen red deer semen |
| CN103518706A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe |
| CN104585164A (en) * | 2015-02-06 | 2015-05-06 | 塔里木大学 | Improved diluent for long-term preservation of semen of ruminant |
-
2018
- 2018-03-27 CN CN201810324076.8A patent/CN110301430A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101084926A (en) * | 2007-07-02 | 2007-12-12 | 高庆华 | Diluent for preserving frozen red deer semen |
| CN103518706A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe |
| CN104585164A (en) * | 2015-02-06 | 2015-05-06 | 塔里木大学 | Improved diluent for long-term preservation of semen of ruminant |
Non-Patent Citations (2)
| Title |
|---|
| 孟娜娜 等: "绵羊精液4℃液态保存的稀释液配方优化研究", 《中国畜牧杂质》 * |
| 宏伟 等: "LDL对绵羊精子冷冻保存效果的研究", 《畜牧与饲料科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115119830A (en) * | 2022-08-02 | 2022-09-30 | 塔里木大学 | Preparation and use method of cow semen low-temperature preservation diluent |
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