CN110296878A - Demodex folliculorum fluorescent staining liquid - Google Patents
Demodex folliculorum fluorescent staining liquid Download PDFInfo
- Publication number
- CN110296878A CN110296878A CN201910740350.4A CN201910740350A CN110296878A CN 110296878 A CN110296878 A CN 110296878A CN 201910740350 A CN201910740350 A CN 201910740350A CN 110296878 A CN110296878 A CN 110296878A
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- fluorescence
- concentration
- demodex folliculorum
- fluorescein
- fluorescent staining
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- Pending
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- 241000193880 Demodex folliculorum Species 0.000 title claims abstract description 29
- 239000007850 fluorescent dye Substances 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 title claims abstract description 14
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 39
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 36
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 24
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims abstract description 18
- COXVTLYNGOIATD-HVMBLDELSA-N CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O Chemical compound CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O COXVTLYNGOIATD-HVMBLDELSA-N 0.000 claims abstract description 10
- 229960003699 evans blue Drugs 0.000 claims abstract description 10
- 239000008213 purified water Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 claims description 8
- -1 Uvitex 2B compound Chemical class 0.000 claims description 8
- AIXZBGVLNVRQSS-UHFFFAOYSA-N 5-tert-butyl-2-[5-(5-tert-butyl-1,3-benzoxazol-2-yl)thiophen-2-yl]-1,3-benzoxazole Chemical compound CC(C)(C)C1=CC=C2OC(C3=CC=C(S3)C=3OC4=CC=C(C=C4N=3)C(C)(C)C)=NC2=C1 AIXZBGVLNVRQSS-UHFFFAOYSA-N 0.000 claims description 7
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 6
- 239000011575 calcium Substances 0.000 claims description 6
- 229910052791 calcium Inorganic materials 0.000 claims description 6
- 238000003745 diagnosis Methods 0.000 abstract description 6
- 238000004043 dyeing Methods 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 19
- 230000000694 effects Effects 0.000 description 8
- 201000004624 Dermatitis Diseases 0.000 description 5
- 210000002374 sebum Anatomy 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 210000002421 cell wall Anatomy 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 229920002101 Chitin Polymers 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 210000003780 hair follicle Anatomy 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 230000000306 recurrent effect Effects 0.000 description 2
- 210000001732 sebaceous gland Anatomy 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 229920000832 Cutin Polymers 0.000 description 1
- 241001127981 Demodicidae Species 0.000 description 1
- 208000020693 Demodicidosis Diseases 0.000 description 1
- 206010012442 Dermatitis contact Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000004343 Indigofera suffruticosa Species 0.000 description 1
- 208000009675 Perioral Dermatitis Diseases 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 206010039792 Seborrhoea Diseases 0.000 description 1
- 206010043189 Telangiectasia Diseases 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- VEQOALNAAJBPNY-UHFFFAOYSA-N antipyrine Chemical compound CN1C(C)=CC(=O)N1C1=CC=CC=C1 VEQOALNAAJBPNY-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 208000037976 chronic inflammation Diseases 0.000 description 1
- 230000006020 chronic inflammation Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 208000010247 contact dermatitis Diseases 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 239000006081 fluorescent whitening agent Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 229960005222 phenazone Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 208000008742 seborrheic dermatitis Diseases 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000009056 telangiectasis Diseases 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
- 210000000216 zygoma Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Abstract
The present invention provides demodex folliculorum fluorescent staining liquid, more particularly to demodex folliculorum detection technique field, including purified water, dimethyl sulfoxide, glycerol, fluorescein, potassium hydroxide and Evans blue, the dimethyl sulfoxide volumetric concentration is 10%-50%, the glycerol volumetric concentration is 10%-50%, and the fluorescein mass concentration is 2mg/ml-10mg/ml, and the potassium hydroxide quality concentration is 0.05g/ml-0.2g/ml, the Evans blue mass concentration is 5mg/ml-50mg/ml, and surplus is purified water.Operation of the present invention is simple, and accurately, the dyeing used time is short, is observable in one minute, improves diagnosis efficiency.
Description
Technical field
The invention belongs to demodex folliculorum detection technique fields, and in particular to demodex folliculorum fluorescent staining liquid.
Background technique
Demodex folliculorum is also known as vermiform mite or follicle mites, the hair follicle and sebaceous glands of people is parasitized, with epithelial cell, gland cell and skin
Rouge is nutrition, and caused skin chronic inflammation is referred to as folliclo mite dermatitis or demodicidosis.Demodex folliculorum multiparasitization is sent out in sebaceous glands
The face reached is especially more common in nose, cheekbone, cheek, lower jaw etc..Clinical manifestation is the flush of persistence, recurrent exerbation, slightly
Telangiectasis, often with burn feeling and shouting pain, symptom is become apparent from when heat meets light.It is more common in 20-50 years old women, often by mistaken diagnosis
The delay treatment for Other diseases.There is similitude in view of its clinical symptoms and following disease, so when clinical diagnosis is rose Cuo
The unknown Facial Recurrent Dermatitis of sore, the steroid dependent dermatitis for having no clearly long-term external application Hormonal ointment history, reason is controlled repeatly repeatly
The contact dermatitis of face of hair, the berloque dermatitis without clear anaphylactogen, the heavier seborrhea of symptom, Perioral Dermatitis, wine
When slag nose, acne and other diseases, it should suggest carrying out demodex folliculorum detection to patient, a possibility that exclude hair follicle insect infection.
Rapidly and accurately it is suitble to treatment most important selection the reason of diagnosis infection, clinically common trichocryptosis is examined
Disconnected main foundation clinical manifestation and the direct microscopy of microscope, operation is not easy, and detection speed is slow, is unfavorable for timely diagnosis and treatment.It is micro-
The direct microscopy positive rate of mirror is low, and accuracy rate is low, and background impurities interference is more.Inspection method is limited to the quick of clinician's inspection sample
The detection level of perception, the experience of materials person and laboratory physician, and time-consuming, is unfavorable for the treatment of patient, especially gives severe disease
The treatment of people brings serious harm.
Therefore, it is badly in need of a kind of demodex folliculorum fluorescent staining liquid for being able to solve existing issue.
Summary of the invention
The object of the present invention is to provide demodex folliculorum fluorescent staining liquid, the fluorescein in the demodex folliculorum fluorescent staining liquid will be combined
β-polysaccharide on demodex folliculorum cell wall, it can be seen that the fluorescence generated under specific wavelength exciting light, facilitates under fluorescence microscope
Clearer observation demodex folliculorum structure.Operation of the present invention is simple, and accurately, the dyeing used time is short, is observable in one minute, improves
Diagnosis efficiency.
The present invention provides the following technical solutions:
Demodex folliculorum fluorescent staining liquid, including purified water, dimethyl sulfoxide, glycerol, fluorescein, potassium hydroxide and ivens
Indigo plant, the dimethyl sulfoxide volumetric concentration are 10%-50%, and the glycerol volumetric concentration is 10%-50%, the fluorescent quality
Amount concentration is 2mg/ml-10mg/ml, and the potassium hydroxide quality concentration is 0.05g/ml-0.2g/ml, the Evans blue matter
Amount concentration is 5mg/ml-50mg/ml, and surplus is purified water.
It preferably, further include 4-AA, the 4-AA mass concentration is 0.02g/ml-
0.1g/ml.4-AA can also develop the color under alkaline condition, reinforce reaction.
Preferably, the fluorescein be the white M2R of fluorescence in Uvitex 2B compound or calcium fluorescence it is white.The white M2R of fluorescence is
One group of compound for being referred to as fluorescent whitening agent or brilliant white agent or bleaching agent.Including Uvitex 2B compound, the compound
Extensive utilization has been obtained in papermaking, weaving and related industries, be mainly used for paper and textile fabric brighten and anti-main deformation
It is yellow.Itself and beta 1-3 and beta 1-4 polysaccharide, such as cellulose and chitin can swash when with ultraviolet and violet exposure
Issue the fluorescence of light blue/white.Uvitex 2B is white (CFW) relative to calcium fluorescence, and effect is identical, cheap, with extensive.
Preferably, when the fluorescein is the Uvitex 2B compound in the white M2R of fluorescence, the dimethyl sulfoxide volume
Concentration is 35%, and the glycerol volumetric concentration is 35%, and the Uvitex 2B mass concentration is 2mg/ml, the potassium hydroxide
Mass concentration is 0.1g/ml, and the Evans blue mass concentration is 10mg/ml, and the 4-AA mass concentration is
0.4g/ml, surplus are purified water.
Preferably, when fluorescein is that calcium fluorescence is white, it can be seen that being produced under the exciting light of 380nm-420nm under fluorescence microscope
Raw fluorescence, when fluorescein is the Uvitex 2B compound in the white M2R of fluorescence, it can be seen that being in wavelength under fluorescence microscope
The fluorescence generated under 480nm exciting light.
In the present invention demodex folliculorum fluorescent staining liquid be it is a kind of containing fluorescein and inhibit background fluorescence staining reagent it is compound
Solution.If containing demodex folliculorum in sample to be measured, fluorescein will combine β-polysaccharide on demodex folliculorum cell wall, such as chitin and fibre
Element etc. is tieed up, it can be seen that the fluorescence generated under specific wavelength exciting light under fluorescence microscope.KOH can be used for dissolving cutin and skin
Rouge facilitates clearer observation demodex folliculorum structure.
The beneficial effects of the present invention are:
It is of the invention innovative using fluorescein with β-polysaccharide on demodex folliculorum cell wall in conjunction with, it is glimmering for calcium using fluorescein
Light is white, the use of fluorescein is the white M2R of fluorescence it can be seen that generating fluorescence under the exciting light of 380nm-420nm under fluorescence microscope
In Uvitex 2B compound, the fluorescence that generates under fluorescence microscope it can be seen that in the case where wavelength is 480nm exciting light, thus
Detect demodex folliculorum.It is easy to operate, be conducive to timely diagnosis and treatment.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is the effect picture that the reagent 1 in the embodiment of the present invention 2 detects.
Specific embodiment
Embodiment 1
The preparation of fluorescent dyeing reagent
Reagent 1: dimethyl sulfoxide, glycerol, Uvitex 2B, potassium hydroxide, Evans blue and 4- ammonia are added in purified water
Base antipyrine sufficiently dissolves, obtains that dimethyl sulfoxide volumetric concentration is 35%, glycerol volumetric concentration is 35%, Uvitex 2B
Mass concentration 2mg/ml, potassium hydroxide quality concentration are 0.1g/ml, Evans blue mass concentration is 10mg/ml, and 4- amino peace is replaced
Than the mixed solution that woods mass concentration is 0.4g/ml.
Reagent 2: dimethyl sulfoxide, glycerol, Uvitex 2B, potassium hydroxide and Evans blue are added in purified water, sufficiently
Dissolution, obtain dimethyl sulfoxide volumetric concentration be 35%, glycerol volumetric concentration is 35%, Uvitex 2B mass concentration 2mg/ml,
The mixed solution that potassium hydroxide quality concentration is 0.1g/ml, Evans blue mass concentration is 10mg/ml.
Embodiment 2
The staining versus of sebum sample
2 parts of sebum samples for having demodex folliculorum to infect are chosen, are respectively placed on glass slide, are contaminated respectively with reagent 1,2
Color dyes 1 minute, in addition coverslip would indicate that blue or blue-green polypide then in fluorescence microscopy microscopic observation demodex folliculorum.
Two kinds of reagents can dye the demodex folliculorum in sebum sample as the result is shown, qualified products be belonged to, now by the two
Contrast effect, as shown in table 1:
The staining versus of sebum sample
| Reagent 1 | Reagent 2 | |
| Dyeing effect | It is good | Difference |
| Developing time | It is long | It is long |
| There is free from admixture | Nothing | Nothing |
| Fluorescent brightness | It is bright | Secretly |
Table 1
From upper result it is found that the effect of reagent 1 is more preferable.
Embodiment 3
Stability of solution is investigated
By reagent 1, reagent 2 is placed in room temperature 6 months, then dyes to sebum sample.Judge to try from dyeing effect
The stability of agent, the results are shown in Table 2:
The stability test of reagent 1 and reagent 2
| Reagent 1 | Reagent 2 | |
| Dyeing effect | It is good | Difference |
| Precipitated crystal | Nothing | Nothing |
| Appearance color | It is constant | It is constant |
Table 2
The result shows that reagent 1,2 appearance color of reagent are constant after being placed at room temperature for placement 6 months, no precipitated crystal is existing
As reagent is able to maintain stabilization, and wherein 1 effect of reagent is best.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (5)
1. demodex folliculorum fluorescent staining liquid, which is characterized in that including purified water, dimethyl sulfoxide, glycerol, fluorescein, potassium hydroxide
And Evans blue, the dimethyl sulfoxide volumetric concentration are 10%-50%, the glycerol volumetric concentration is 10%-50%, the fluorescence
Plain mass concentration is 2mg/ml-10mg/ml, and the potassium hydroxide quality concentration is 0.05g/ml-0.2g/ml, the ivens
Blue mass concentration is 5mg/ml-50mg/ml, and surplus is purified water.
2. demodex folliculorum fluorescent staining liquid according to claim 1, which is characterized in that it further include 4-AA, institute
Stating 4-AA mass concentration is 0.02g/ml-0.1g/ml.
3. demodex folliculorum fluorescent staining liquid according to claim 2, which is characterized in that the fluorescein is in the white M2R of fluorescence
Uvitex 2B compound or calcium fluorescence it is white.
4. demodex folliculorum fluorescent staining liquid according to claim 3, which is characterized in that the fluorescein is in the white M2R of fluorescence
Uvitex 2B compound when, the dimethyl sulfoxide volumetric concentration be 35%, the glycerol volumetric concentration be 35%, it is described
Uvitex 2B mass concentration is 2mg/ml, and the potassium hydroxide quality concentration is 0.1g/ml, the Evans blue mass concentration
For 10mg/ml, the 4-AA mass concentration is 0.4g/ml, and surplus is purified water.
5. demodex folliculorum fluorescent staining liquid according to claim 1, which is characterized in that when the fluorescein is that calcium fluorescence is white,
It can be seen that generating fluorescence under the exciting light of 380nm-420nm under fluorescence microscope；The fluorescein is in the white M2R of fluorescence
When Uvitex 2B compound, it can be seen that the fluorescence generated in the case where wavelength is 480nm exciting light under fluorescence microscope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910740350.4A CN110296878A (en) | 2019-08-12 | 2019-08-12 | Demodex folliculorum fluorescent staining liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910740350.4A CN110296878A (en) | 2019-08-12 | 2019-08-12 | Demodex folliculorum fluorescent staining liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110296878A true CN110296878A (en) | 2019-10-01 |
Family
ID=68032947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910740350.4A Pending CN110296878A (en) | 2019-08-12 | 2019-08-12 | Demodex folliculorum fluorescent staining liquid |
Country Status (1)
| Country | Link |
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| CN (1) | CN110296878A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1503909A (en) * | 2001-04-12 | 2004-06-09 | ������������ʽ���� | Specimen analyzing implement |
| CN106323925A (en) * | 2016-08-11 | 2017-01-11 | 付微 | Fluorescence dye for detecting fungus and dermatozoon |
| CN108168983A (en) * | 2018-03-05 | 2018-06-15 | 江苏诺鬲生物科技有限公司 | A kind of fluorescent reagent of fungi specific stain |
| CN109297790A (en) * | 2018-10-26 | 2019-02-01 | 江西业力医疗器械有限公司 | A kind of fluorescent staining liquid detecting fungi |
| CN110029142A (en) * | 2018-12-29 | 2019-07-19 | 江西业力医疗器械有限公司 | A kind of two Methods for Fungi Detection based on liquid-based tabletting technology |
-
2019
- 2019-08-12 CN CN201910740350.4A patent/CN110296878A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1503909A (en) * | 2001-04-12 | 2004-06-09 | ������������ʽ���� | Specimen analyzing implement |
| CN106323925A (en) * | 2016-08-11 | 2017-01-11 | 付微 | Fluorescence dye for detecting fungus and dermatozoon |
| CN108168983A (en) * | 2018-03-05 | 2018-06-15 | 江苏诺鬲生物科技有限公司 | A kind of fluorescent reagent of fungi specific stain |
| CN109297790A (en) * | 2018-10-26 | 2019-02-01 | 江西业力医疗器械有限公司 | A kind of fluorescent staining liquid detecting fungi |
| CN110029142A (en) * | 2018-12-29 | 2019-07-19 | 江西业力医疗器械有限公司 | A kind of two Methods for Fungi Detection based on liquid-based tabletting technology |
Non-Patent Citations (1)
| Title |
|---|
| 戴维丝•拉荣: "《医学重要真菌鉴定指南》", 30 September 2016 * |
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Application publication date: 20191001 |