CN110292585A - For treating the intestinal flora capsule and preparation method that crystal methamphetamine habituation is given up - Google Patents
For treating the intestinal flora capsule and preparation method that crystal methamphetamine habituation is given up Download PDFInfo
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- CN110292585A CN110292585A CN201910745092.9A CN201910745092A CN110292585A CN 110292585 A CN110292585 A CN 110292585A CN 201910745092 A CN201910745092 A CN 201910745092A CN 110292585 A CN110292585 A CN 110292585A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
Abstract
The present invention relates to caprophyl capsule technique fields, the intestinal flora capsule and preparation method that crystal methamphetamine habituation is given up more particularly, to are treated, which is that acquisition healthy population fresh excreta and sterile saline stir obtained suspension, filtering, remove residue, obtained intestinal flora bacterium solution；Using special culture medium, Ruminococcus albus and yellow Ruminococcus culture are extracted from intestinal flora bacterium solution, obtains target bacterium solution.CaCl is added into target bacterium solution2, then the glue configured with sodium alginate and pectin mix, carries out chemical crosslink reaction at room temperature, filtering cleans, obtains intestinal flora particle, intestinal flora particle is wrapped in capsule, obtain intestinal flora capsule, be protected from light and be stored at room temperature.The intestinal flora capsule that the present invention is prepared can adjust addict's intestinal flora composition, realize giving up for p-Methylamphetamine drug addiction, gradually decrease craving of the addict to drugs, and regulation addict's intestinal microecology balance effectively improves drug rehabilitation rate.
Description
Technical field
The present invention relates to caprophyl capsule technique fields, more particularly, to treat the enterobacteriaceae that crystal methamphetamine habituation is given up
Group's capsule and preparation method.
Background technique
Various microorganisms present in environment are referred to as intestinal flora in human body intestinal canal.Report display, about 10,000,000,000,000
A bacterium parasitizes in human body intestinal canal, which is about 10 times of human body cell sum, including Escherichia coli, lactic acid bacteria, bifid
Bacillus, peptococcus, streptococcus etc..These bacterium colonies in cradle, just in human body, largely inhabit big in human body by parasitism
In intestines, they influence each other maintains the dynamic equilibrium of intestinal microenvironment jointly.The study found that being deposited between mammalian brain and enteron aisle
In two-way communication, this nerve-endocrine net for connecting gastrointestinal tract and central nervous system in different levels
Network be referred to as nervous system in intestines-brain axis, including central nervous system, autonomic nerves system, enteron aisle, neuroendocrine system,
The structures such as immune system.Brain can influence functions of intestines and stomach by this axis, and stomach and intestine activity can also generate shadow through this shaft assignment pivot nerve
It rings, to realize, close-connected two-way neurohumor is exchanged between gastrointestinal tract and brain.
Numerous pairs of intestinal floras studies have shown that intestinal bacilli illness (bacterial community structural dynamics in intestinal tract or functional structure occur
Variation) it can result in the generations of various diseases, such as obesity, diabetes, the nervous system disease, allergy and various inflammation.Enteron aisle
The variation of flora fauna composition or density can increase what the mankind and mouse model reacted multiple pathogens and abnormal mucosa-immune
Sensibility.For example, the change of mouse intestinal micropopulation caused by antibiotic and metabolin increases the easy of clostridium difficile infection
Perception.Recent research shows that there is also be associated with drug addiction for intestinal flora.Foreign study found that the item of crystal methamphetamine induction
Compared with normal rat significant changes, including bacterial diversity and strain occur for part Place Preference rat, microflora feature
Composition, these variations may influence the release of thrombocytin and the function of microglia in rat body, and participate in crystal methamphetamine
Caused cognitive impairment process.(FULCHER J A, HUSSAIN S K, the COOK R, et such as Fulcher in human research
al.Effects of Substance Use and Sex Practices on the Intestinal Microbiome
During HIV-1Infection [J] .J Infect Dis, 2018,218 (10): 1560-1570), it was also found that addict's intestines
Ruminococcus (including Ruminococcus albus and yellow Ruminococcus) in road, Collins Bordetella, secondary bar shape Pseudomonas abundance
It reduces to exist with crystal methamphetamine abuse and be associated with, and the increase of the above flora abundance is advantageously possible for giving up for drug addiction.
Intestinal flora has as various diseases such as treatment diarrhea, vaginal yeast infection, urinary tract infections, irritable bowel syndrome
Long history.Currently, intestinal flora transplanting be mainly used in recurrent clostridium difficile infection, Crohn disease and
The treatment of inflammatory bowel disease.Flora transplanting refers to the fecal specimens from donor is transferred in subject's body, so as to improve by
The distribution of examination person's intestinal flora.Intestinal flora transplanting is main to be carried out by two ways: it is a kind of similar to enema, by fecal specimens
It is injected directly into subject's anus；Another kind is that fecal specimens are imported subject's enteron aisle depths by pipe.In certain feelings
Under condition, intestinal flora transplanting becomes the unique method for treating certain diseases.Intestinal flora transplanting in recent years has obtained U.S.'s food
Drug Administration (FDA) approval, many cases patient have obtained good therapeutic effect through intestinal flora transplanting.Currently, treatment intestines
Road imbalance and restorative enteron aisle stable state have become the critical treatment strategy for the treatment of disease.
In short, important component of the intestinal flora as human body, is closely related with body health, existing research at present is seen
It observes Ruminococcus to be associated with drug habit presence, but does not have also and guard against the Intestinal flora graft application in crystal methamphetamine habituation
Disconnected report.It can inhibit crystal methamphetamine habituation therefore, it is necessary to a kind of, the intestinal flora capsule of drug relapse rate is effectively reduced.
Summary of the invention
Based on the above issues, the enterobacteriaceae that the object of the present invention is to provide a kind of to give up for treating crystal methamphetamine habituation
Group's capsule and preparation method thereof, addict takes the intestinal flora capsule, can adjust addict's intestinal flora composition, realization pair
Drug addiction is given up, and is gradually recovered addict's intestinal microecology balance, effectively improves drug rehabilitation rate.
The present invention solves above-mentioned technical problem by following technological means:
The intestinal flora glue that one of the objects of the present invention is to provide a kind of to give up for treating crystal methamphetamine habituation
The preparation method of capsule, comprising the following steps:
S1. healthy population fresh excreta is acquired, sealed bag is sealed, spare；
S2. the fresh excreta of acquisition is stirred and evenly mixed with sterile saline, suspension is made, suspension was carried out
Filter removes residue, obtains intestinal flora bacterium solution, be placed in 2~5 DEG C of refrigerator and save, spare；
S3. Ruminococcus albus (Ruminococcus albus) and/or yellow cud are extracted from intestinal flora bacterium solution
Coccus (Ruminococcus flavefaciens), culture, obtains target bacterium solution；
S4. sodium alginate and pectin is taken to be added in distilled water, stirring and dissolving obtains glue；It is added into target bacterium solution
CaCl2, mixed solution is obtained, glue is added in mixed solution, carries out chemical crosslink reaction at room temperature, filtering cleaning obtains
To intestinal flora particle；
S5. intestinal flora particle is wrapped in capsule, obtains intestinal flora capsule, is protected from light and is stored at room temperature.
The healthy population of above-mentioned acquisition fresh excreta need to meet the following conditions:
A. the relatives of young crowd or addict are preferential, and especially the relatives of addict have similar micro- with addict
Ecological environment, but needing to meet not in contact with any addictive substance includes the conditions such as drink, smoke and take drugs；
B. diarrhea does not occur over the past half year closely；
C. antibiotic was not used in this year, antibacterials and other are not used in nearest 3 months causes enteron aisle micro-
The drug of ecological disorder；
D. without any of communicable disease, such as hepatitis A, hepatitis B, hepatitis etc.；
E. without the disease of digestive systems such as gastroenteric tumor, polyp or intestinal surgery history；
F. without any chronic disease, such as diabetes, coronary heart disease, hypertension.
Further, the step S3 specifically: intestinal flora bacterium solution is applied into plate on RGCA solid medium, cultivates 24
After hour, by the colony inoculation grown in RGCA to the culture tube containing RGCA liquid culture medium, it is placed on shaking table and cultivates
10~14h obtains target bacterium solution.RGCA solid medium is mainly used for the isolation and culture to rumen microorganism.
Further, the RGCA solid medium includes rumen fluid 30%, glucose 1%, cellobiose 1%, starch
1%, agar 15%.
Further, the RGCA liquid culture medium includes rumen fluid 30%, glucose 1%, cellobiose 1%, starch
1%.
Further, the sodium chloride solution that the concentration of the sterile saline is 0.9%, the fresh excreta and sterile life
Salt water is managed according to 50g:(150mL~300mL).
Further, the glue total concentration is 5%, and the mass ratio of the sodium alginate and pectin is 2:1.
Further, the CaCl in the mixed solution2Concentration is 0.3mol/L.
Further, the glue with 0.3~0.5mL/min of flow velocity by EFI to stirring in mixed solution in, the glue
The volume ratio of liquid and mixed solution is 1:1.
The second object of the present invention is the provision of the intestinal flora capsule that above-mentioned preparation method is prepared.
The third object of the present invention is the provision of above-mentioned intestinal flora capsule in crystal methamphetamine habituation withdrawal and treatment
Application, after taking the intestinal flora capsule, by regulate and control intestinal flora, realization drugs are given up.
The present invention mainly using in the healthy population excrement that is screened through multinomial stringent condition with inhibit crystal methamphetamine drugs
The relevant intestinal flora Ruminococcus albus of habituation and/or yellow Ruminococcus, it is mixed with sodium alginate and pectin and is first made
It is fitted into capsule shells at intestinal flora particle, then by intestinal flora particle, intestinal flora capsule is made, by taking orally the enterobacteriaceae
Group's capsule carries out flora transplanting, achievees the purpose that p-Methylamphetamine addict's drugs stage, has nonhazardous, permanently effective
The multiple advantages such as property, withdrawal rate height.
The method that the present invention uses fungus treatment, according to the difference of crystal methamphetamine habituation crowd and Normal Human Intestinal flora
It is different, effect of the difference flora in drug addiction is analyzed, by increasing intestinal flora relevant to the inhibition of crystal methamphetamine drugs
Ruminococcus albus and/or yellow Ruminococcus realize the Tiny ecosystem therapy having no toxic side effect, and with the shape of intestinal flora capsule
Formula is treated, and craving of the crystal methamphetamine addict to drugs is reduced, and is improved body intestinal microecology balance, is effectively mentioned
High drug rehabilitation rate.
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this explanation
Content disclosed by book understand easily of the invention other a little with effect.The present invention can also be by addition different specific
Embodiment is embodied or practiced, and the various details in this specification can also not carried on the back based on different viewpoints and application
From carrying out various modifications or alterations under spirit of the invention.It should be clear that the process equipment or dress that are not indicated specifically in the following example
It sets and is all made of conventional equipment or device in the art.In addition, unless otherwise indicated, the number of various method steps is only to identify respectively
The convenient tool of method and step, rather than for the arrangement order of limitation various method steps or limit the scope of the invention,
Relativeness is altered or modified, without material changes in technical content, when being considered as the enforceable scope of the present invention.
It should be noted that healthy population fresh excreta is acquired in preparation method of the invention, healthy population therein
Screening conditions are as follows:
A. the relatives of young crowd or addict are preferential, and especially the relatives of addict have similar micro- with addict
Ecological environment, but needing to meet not in contact with any addictive substance includes the conditions such as drink, smoke and take drugs；
B. diarrhea does not occur over the past half year closely；
C. antibiotic was not used in this year, antibacterials and other are not used in nearest 3 months causes enteron aisle micro-
The drug of ecological disorder；
D. without any of communicable disease, such as hepatitis A, hepatitis B, hepatitis etc.；
E. without the disease of digestive systems such as gastroenteric tumor, polyp or intestinal surgery history；
F. without any chronic disease, such as diabetes, coronary heart disease, hypertension.
Solution and culture medium prepare:
The sterile saline that concentration is 0.9%: it takes sodium chloride to be added in distilled water and stirs to being completely dissolved, configuration obtains
The physiological saline that concentration is 0.9% carries out sterilizing processing to the physiological saline, obtains sterile saline, spare.
RGCA solid medium: each raw material: rumen fluid 30%, glucose 1%, fibre is taken respectively according to following mass percent
Disaccharides 1%, starch 1%, agar 15% are tieed up, is mixed with to obtain RGCA solid medium.
RGCA liquid culture medium: each raw material: rumen fluid 30%, glucose 1%, fibre is taken respectively according to following mass percent
Disaccharides 1%, starch 1% are tieed up, is mixed with to obtain RGCA liquid culture medium.
Isolation and culture is carried out to rumen microorganism using RGCA solid medium in the present invention, with reference to " Chinese milk cow "
2009 the 4th the 18-23 pages of the phase, " separation, identification and the quantitative analysis skill of rumen microorganism that Sun Jianfeng, Lin Yingting are delivered
Art ".
Embodiment 1
The intestinal flora capsule of the present embodiment the preparation method is as follows:
S1. healthy population fresh excreta is acquired, is sealed with sealed bag, it is spare.
S2. the fresh excreta 50g of S1 acquisition is weighed, 150mL sterile saline is measured, by weighed fresh excreta and nothing
Bacterium physiological saline stirs and evenly mixs, and obtains suspension, is filtered using 3 layers of gauze to suspension, removes residue, obtains enterobacteriaceae
Group's bacterium solution.Intestinal flora bacterium solution is placed in 2~5 DEG C of refrigerator and is saved, it is spare.
S3. intestinal flora bacterium solution is applied into plate on RGCA solid medium, after culture 24 hours, by RGCA solid culture
In the colony inoculation grown in base to the culture tube containing RGCA liquid culture medium, it is placed on shaking table and cultivates 12h, obtain target
Bacterium solution extracts Ruminococcus albus or yellow Ruminococcus from intestinal flora bacterium solution, i.e., containing white tumor in target bacterium solution
Abrasive net-balloon for stomach bacterium or yellow Ruminococcus.
S4. the mass ratio 2:1 for pressing sodium alginate and pectin, weighs sodium alginate and pectin, by weighed seaweed respectively
Sour sodium and pectin are added in distilled water, the glue that stirring and dissolving obtains sodium alginate and pectin quality total concentration is 5%；In every liter
The CaCl of 0.3mol is added in target bacterium solution2, stirring and dissolving obtains mixed solution；With the flow velocity of 0.3mL/min by glue EFI
In mixed solution into quick stirring, and the volume ratio of the glue and mixed solution sprayed into be 1:1, glue and mixed solution in
After carrying out chemical crosslink reaction 30min at room temperature, filtering cleaning obtains intestinal flora particle.
S5. intestinal flora particle is wrapped in capsule, obtains intestinal flora capsule, is protected from light and is stored at room temperature.
Embodiment 2
The intestinal flora capsule of the present embodiment the preparation method is as follows:
S1. healthy population fresh excreta is acquired, is sealed with sealed bag, it is spare.
S2. the fresh excreta 50g of S1 acquisition is weighed, 300mL sterile saline is measured, by weighed fresh excreta and nothing
Bacterium physiological saline stirs and evenly mixs, and obtains suspension, and suspension is transferred in sterile centrifugation tube, in 2500~3000xg of centrifugal force,
It is centrifuged 20min under conditions of 20~25 DEG C of temperature, takes out the supernatant in sterile centrifugation tube, obtains intestinal flora bacterium solution.By intestines
Road flora bacterium solution is placed in 2~5 DEG C of refrigerator and saves, spare.
S3. intestinal flora bacterium solution is applied into plate on RGCA solid medium, after culture 24 hours, by RGCA solid culture
In the colony inoculation grown in base to the culture tube containing RGCA liquid culture medium, it is placed on shaking table and cultivates 12h, obtain target
Bacterium solution extracts Ruminococcus albus or yellow Ruminococcus from intestinal flora bacterium solution, i.e., contain white in target bacterium solution
Ruminococcus or yellow Ruminococcus.
S4. the mass ratio 2:1 for pressing sodium alginate and pectin, weighs sodium alginate and pectin, by weighed seaweed respectively
Sour sodium and pectin are added in distilled water, the glue that stirring and dissolving obtains sodium alginate and pectin quality total concentration is 5%；In every liter
The CaCl of 0.3mol is added in target bacterium solution2, stirring and dissolving obtains mixed solution；With the flow velocity of 0.5mL/min by glue EFI
In mixed solution into quick stirring, and the volume ratio of the glue and mixed solution sprayed into be 1:1, glue and mixed solution in
After carrying out chemical crosslink reaction 30min at room temperature, filtering cleaning obtains intestinal flora particle.
S5. intestinal flora particle is wrapped in capsule, obtains intestinal flora capsule, is protected from light and is stored at room temperature.
Embodiment 3
The intestinal flora capsule of the present embodiment the preparation method is as follows:
S1. healthy population fresh excreta is acquired, is sealed with sealed bag, it is spare.
S2. the fresh excreta 50g of S1 acquisition is weighed, 200mL sterile saline is measured, by weighed fresh excreta and nothing
Bacterium physiological saline stirs and evenly mixs, and obtains suspension, and suspension is transferred in sterile centrifugation tube, in 2500~3000xg of centrifugal force,
Low-speed centrifugal 20min under conditions of 20~25 DEG C of temperature, the supernatant in sterile centrifugation tube after taking out low-speed centrifugal, by supernatant
Liquid is transferred in new sterile centrifugation tube, the high speed centrifugation under conditions of 6000~8000xg of centrifugal force, 15~20 DEG C of temperature
30min, the supernatant in sterile centrifugation tube after taking out high speed centrifugation, obtains intestinal flora bacterium solution.Intestinal flora bacterium solution is set
It is saved in 2~5 DEG C of refrigerator, it is spare.
S3. intestinal flora bacterium solution is applied into plate on RGCA solid medium, after culture 24 hours, by RGCA solid culture
In the colony inoculation grown in base to the culture tube containing RGCA liquid culture medium, it is placed on shaking table and cultivates 12h, obtain target
Bacterium solution extracts Ruminococcus albus or yellow Ruminococcus from intestinal flora bacterium solution, i.e., contain white in target bacterium solution
Ruminococcus and/or yellow Ruminococcus.
S4. the mass ratio 2:1 for pressing sodium alginate and pectin, weighs sodium alginate and pectin, by weighed seaweed respectively
Sour sodium and pectin are added in distilled water, the glue that stirring and dissolving obtains sodium alginate and pectin quality total concentration is 5%；In every liter
The CaCl of 0.3mol is added in target bacterium solution2, stirring and dissolving obtains mixed solution；With the flow velocity of 0.4mL/min by glue EFI
In mixed solution into quick stirring, and the volume ratio of the glue and mixed solution sprayed into be 1:1, glue and mixed solution in
After carrying out chemical crosslink reaction 30min at room temperature, filtering cleaning obtains intestinal flora particle.
The present invention is mainly using relevant to drug addiction is inhibited in the healthy population excrement screened through multinomial stringent condition
Intestinal flora, and capsule is made, flora transplanting is carried out by oral intestinal canal flora capsule, reaches p-Methylamphetamine addict's poison
The purpose that product are given up has the multiple advantages such as nonhazardous, long-term effectiveness, withdrawal rate height.
It is described in detail, those skilled in the art should understand that, it can modify to technical solution of the present invention
Or equivalent replacement should all cover without departing from the objective and range of technical solution of the present invention in claim of the invention
In range.Technology not described in detail in the present invention, shape, construction portion are well-known technique.
Claims (10)
1. the preparation method for treating the intestinal flora capsule that crystal methamphetamine habituation is given up, which is characterized in that including following
Step:
S1. healthy population fresh excreta is acquired, sealed bag is sealed, spare；
S2. the fresh excreta of acquisition is stirred and evenly mixed with sterile saline, suspension is made, suspension is filtered, is gone
Except residue, intestinal flora bacterium solution is obtained, is placed in 2~5 DEG C of refrigerator and saves, it is spare；
S3. RGCA culture medium is used, Ruminococcus albus and/or yellow Ruminococcus are extracted from intestinal flora bacterium solution, is cultivated,
Obtain target bacterium solution；
S4. sodium alginate and pectin is taken to be added in distilled water, stirring and dissolving obtains glue；CaCl is added into target bacterium solution2, obtain
To mixed solution, glue is added in mixed solution, carries out chemical crosslink reaction at room temperature, filtering cleaning obtains enterobacteriaceae
Group's particle；
S5. intestinal flora particle is wrapped in capsule, obtains intestinal flora capsule, is protected from light and is stored at room temperature.
2. it is according to claim 1 for treating the preparation method for the intestinal flora capsule that crystal methamphetamine habituation is given up,
It is characterized in that, the step S3 specifically: intestinal flora bacterium solution is applied plate on RGCA solid medium, is cultivated 24 hours
Afterwards, will the colony inoculation that grown in RGCA in the culture tube containing RGCA liquid culture medium, be placed in culture 10 on shaking table~
14h obtains target bacterium solution.
3. it is according to claim 2 for treating the preparation method for the intestinal flora capsule that crystal methamphetamine habituation is given up,
It is characterized in that, the RGCA solid medium includes rumen fluid 30%, glucose 1%, cellobiose 1%, starch 1%, fine jade
Rouge 15%.
4. it is according to claim 3 for treating the preparation method for the intestinal flora capsule that crystal methamphetamine habituation is given up,
It is characterized in that, the RGCA liquid culture medium includes rumen fluid 30%, glucose 1%, cellobiose 1%, starch 1%.
5. it is according to claim 1 for treating the preparation method for the intestinal flora capsule that crystal methamphetamine habituation is given up,
It is characterized in that, the sodium chloride solution that the concentration of the sterile saline is 0.9%, the fresh excreta and sterile physiological salt
Water is according to 50g:(150mL~300mL).
6. it is according to claim 1 for treating the preparation method for the intestinal flora capsule that crystal methamphetamine habituation is given up,
It is characterized in that, the glue total concentration is 5%, the mass ratio of the sodium alginate and pectin is 2:1.
7. it is according to claim 1 for treating the preparation method for the intestinal flora capsule that crystal methamphetamine habituation is given up,
It is characterized in that, the CaCl in the mixed solution2Concentration is 0.3mol/L.
8. it is according to claim 1 for treating the preparation method for the intestinal flora capsule that crystal methamphetamine habituation is given up,
It is characterized in that, the glue with 0.3~0.5mL/min of flow velocity by EFI to stirring in mixed solution in, the glue and
The volume ratio of mixed solution is 1:1.
9. the intestinal flora capsule that -8 any preparation methods are prepared according to claim 1.
10. application of the intestinal flora capsule according to claim 9 in crystal methamphetamine habituation withdrawal and treatment.
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| CN111588709A (en) * | 2020-05-25 | 2020-08-28 | 西南大学 | Construction method and application of sterile mouse model based on methamphetamine induction Parkinson |
| CN111642457A (en) * | 2020-05-25 | 2020-09-11 | 西南大学 | Rat model for methamphetamine intervention pseudo-aseptic, construction method and application |
| WO2021134740A1 (en) * | 2020-01-03 | 2021-07-08 | 四川农业大学 | Tibetan pig fecal microbiota capsule for relieving diarrhea in weaned piglet, and preparation method therefor |
| CN113930526A (en) * | 2021-12-02 | 2022-01-14 | 张聚 | Method and composition for identifying methamphetamine-related people and application of composition |
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Application publication date: 20191001 |