CN110283743A - The degradation bacteria and its application of allelochemical in degrading plant root exudates - Google Patents
The degradation bacteria and its application of allelochemical in degrading plant root exudates Download PDFInfo
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Abstract
The present invention relates to microorganism fields, provide the degradation bacteria and its application of allelochemical in degrading plant root exudates, classification naming is Achromobacter xylosoxidans (Achromobacter xylosoxidans) and Pseudomonas nitroreducens (Pseudomonas nitroreducens), and culture presevation number is respectively CGMCC NO.14517 and CGMCC NO.14518.The Allelochemical degradation bacterium can degrade allelochemical in a variety of degrading plant root exudates.
Description
Technical field
The present invention relates to microorganism field, provides the degradation bacteria of allelochemical in degrading plant root exudates and its answer
It can be degraded a variety of allelochemicals with, the Allelochemical degradation bacterium.
Background technique
Peanut is the important oil plant in China and industrial crops, and the limitation and peanut economic benefit due to Soil Utilization are opposite
It is higher, it is very universal in the main growing area peanut continuous cropping phenomenon of peanut.However, peanut continuous cropping can generate continuous cropping obstacle effect, even
It is longer to make the time limit, peanut yield and quality decline are more severe, have seriously affected the sustainable development of peanut production.Auto toxicity is colored
Raw continuous cropping obstacle one of the major reasons.Auto toxicity refers to that root system secretion and plant stubble release allelochemical produce plant growth
Raw inhibiting effect.The root systems such as peanut, tobacco, cucumber secrete allelochemical to the plant height, root long and root activity of plant shoots all
Inhibiting effect is shown, and inhibition level enhances as allelochemical concentration increases.Forefathers study discovery Peanut Root System point
Secreting allelochemical influences rhizosphere microbial structure of community so as to cause the exacerbation of peanut soil-borne disease.
Currently, the research for peanut Allelochemical degradation bacterium is less.Benzoic acid and its derivative are a kind of important
Allelochemical, detected in various plants root exudates, and early-stage study discovery benzoic acid to peanut germinate
And growth of seedling significantly inhibits, this patent screens benzoic acid degradation from Peanut continuous cropping experimental field healthy plant rhizosphere soil
Bacterium, and its degradation characteristic is studied, resource guarantee and scientific basis are provided to alleviate peanut continuous cropping obstacle using degradation bacteria.
Summary of the invention
To achieve the purpose of the present invention, the technical solution adopted by the present invention is that:
The degradation bacteria of allelochemical in a kind of degrading plant root exudates, which is characterized in that classification naming is xylose oxygen
Change achromobacter (Achromobacter xylosoxidans), it is common to be preserved in China Committee for Culture Collection of Microorganisms
Microorganism center, the deposit date is on August 10th, 2017, culture presevation number was CGMCC NO.14517.
The degradation bacteria of allelochemical in a kind of degrading plant root exudates, which is characterized in that classification naming be nitro also
Former pseudomonad (Pseudomonas nitroreducens), it is common to be preserved in China Committee for Culture Collection of Microorganisms
Microorganism center, the deposit date is on August 10th, 2017, culture presevation number was CGMCC NO.14518.
It is a further object of the present invention to provide application of the above-mentioned degradation bacteria in degradation allelochemical.
It is a further object of the present invention to provide above-mentioned degradation bacterias to alleviate the application in continuous cropping obstacle.
Further, the allelochemical has phenyl ring class or chain saturated fatty acids.
Further, the allelochemical is acetophenone, stearic acid, 3,5- dimethylbenzaldehyde or palmitinic acid.
It is a further object of the present invention to provide a kind of methods of allelochemical of degrading, which is characterized in that uses institute of the present invention
The Achromobacter xylosoxidans (Achromobacter xylosoxidans) stated, degradation condition are as follows: pH 6.0~9.0, temperature
30~45 DEG C, NaCl concentration 0~6%.
It is a further object of the present invention to provide a kind of methods of allelochemical of degrading, which is characterized in that uses institute of the present invention
The Achromobacter xylosoxidans (Achromobacter xylosoxidans) or Pseudomonas nitroreducens stated
(Pseudomonas nitroreducens), degradation condition are as follows: pH 6.0~8.0,30~35 DEG C of temperature, NaCl concentration 0~
4%.
It is a further object of the present invention to provide a kind of microbial inoculum of allelochemical of degrading, the microbial inoculum includes above-mentioned degradation bacteria.
It is a kind of effective measures for overcoming plant Auto toxicity using biodegradation technique, the advantage is that plant growth
It is repaired in the case where not influencing, and be there is no secondary pollution, be a kind of biological renovation method of high-efficiency environment friendly, and screened
It is the key link of this technology with separation allelochemical efficient degrading bacteria.This patent screens two by the method for enrichment culture
Plant height imitates Allelochemical degradation bacterium HJ-2 and HJ-3, is identified as Achromobacter xylosoxidans (Achromobacter
) and Pseudomonas nitroreducens (Pseudomonas nitroreducens) xylosoxidans.
This patent discovery Achromobacter xylosoxidans HJ-2 and Pseudomonas nitroreducens HJ-3 can significantly degrade benzene first
Acid, acetophenone and 3,5- dimethylbenzaldehyde etc. have the change of the chain saturated fatty acids such as phenyl ring class and stearic acid, palmitinic acid
Feel substance, enrich the type of Allelochemical degradation bacterium, provides assured resources guarantee to alleviate plant Auto toxicity.
The height of degradation efficiency is the precondition that can degradation bacteria be applied.Qi Guozhen etc. has screened more plants from apple rhizosphere
Suppression harmful bacteria degradation bacteria, the best degradation bacteria BL2 of degradation efficiency do not have phthalic acid, P-hydroxybenzoic acid, phloridzin, coke
The degradation rate of gallate-based is respectively 66%, 72%, 84% and 84%.What will just etc. has separated 3 plants of benzoic acid degradation bacterias to benzene first
The degradation rate of acid only has 95.32%, 91.63% and 90.15%.The drop of this patent separation degradation bacteria HJ-2 and HJ-3 para Toluic Acid
Solution rate respectively reaches 96.88% and 92.65%, wherein degradation bacteria HJ-2 degradation with higher compared with the degradation bacteria studied
Efficiency.
Degradation bacteria must have stronger adaptive capacity to environment just to have significant practical applications.This patent analyzes two plants of degradation bacterias
To the adaptation situation of the environmental factors such as pH, temperature and NaCl concentration.PH has larger impact, strong acid or highly basic to cell enzyme activity
It will cause albuminous degeneration, destroy cell membrane.It is raw in pH6.0~7.0 that Zhao Dongyue etc. screens 5 plants of ginseng suppression harmful bacteria degradation bacterias
Long status is best, and degradation bacteria HJ-2 appropriate pH is 6.0~9.0 in this patent, and it is 6.0~8.0,2 that degradation bacteria HJ-3, which adapts to pH,
Strain degradation bacteria has preferable pH tolerance range, and degradation bacteria HJ-2 is wider to pH tolerance range；Extremes of temperature can press down
Cell enzyme activity processed, and then the growth of microorganism is influenced, also result in the reduction of Allelochemical degradation bacterium degradation efficiency.Huang Xingru
Several plants of degradation bacteria preference temperatures of degrading polyaromatic hydrocarbon of equal screenings are below 40 DEG C, and degradation bacteria HJ-2 is suitable for temperature in this research
Degree is 30~45 DEG C, has wider temperature range；If NaCl Du Taigao can make microorganism thin in microorganism growing environment
Extracellular boundary's osmotic pressure is excessively high, causes cell water shortage dead；In addition excessive sodium ion can inhibit the activity of cellular enzymes, therefore micro- life
Object has certain NaCl concentration range, and degradation bacteria HJ-2 degradation rate after NaCl concentration is increased to 6% is remarkably decreased, and is dropped
Solution bacterium HJ-3 degradation rate after NaCl concentration is increased to 4% is remarkably decreased, this may be inhibited related by NaCl with cell.
Zhu's magnitude has screened the achromobacter of one plant of degradation naphthalene, is resistant to 1~2%NaCl concentration, degradation bacteria HJ-2 in this patent
It is achromobacter, and it is 0~6% that the bacterium, which is suitable for NaCl concentration, is resistant to higher NaCl concentration, has better environment suitable
It should be able to power.
Peanut Root System secretes a variety of allelochemicals, and allelochemical exists in hybrid form in environment, the degradation bacteria of screening
There can be degradation more to have significant practical applications to other allelochemicals.Some researches show that Micrococcus lylaes, Japanese ardisia leaf
Bacillus and Li Shi strangle the main allelochemicals of peanuts such as minot Salmonella degradation oleic acid, hexadecanoic acid, phthalic acid.This patent screening
Two degradation bacterias can degrade a variety of peanut allelochemicals, especially degradation bacteria HJ-2 degradation capability it is stronger, show that the bacterium is
One plant for preventing and treating the desirable strain of peanut Auto toxicity.
Compared with the existing technology, the application achieve it is following the utility model has the advantages that
(1) 2 plant heights effect allelochemical is separated to from the health peanut rhizosphere soil of Peanut continuous cropping long term experiment base
Degradation bacteria HJ-2 and HJ-3 are respectively Achromobacter xylosoxidans (Achromobacter xylosoxidan s by identification
) and Pseudomonas nitroreducens (Pseudomonas nitroreducens HJ-3) HJ-2.
(2) by single factor experiment to degradation bacteria carry out degradation characteristic measurement, pH be 6.0~9.0, preference temperature be 30~
It is 0~6% that 45 DEG C, which are suitable for NaCl concentration,；Degradation bacteria HJ-3 is suitable for degradation condition pH 6.0~8.0, and preference temperature is 30~35
DEG C, suitable NaCl concentration is 0~4%.
(3) two plants of degradation bacterias can degrade a variety of allelochemicals, and there is wide spectrum to degrade allelochemical characteristic.
Detailed description of the invention
Fig. 1 is the transmission electron microscope picture of degradation bacteria HJ-2 and HJ-3；
Fig. 2 is the phylogenetic tree constructed based on degradation bacteria HJ-2 (A) and degradation bacteria HJ-3 (B) gene order；
Fig. 3 is the influence that different pH, temperature and NaCl concentration grow degradation bacteria HJ-2 and HJ-3.
Specific embodiment
Sample source: from Chinese Academy of Sciences's red soil ecological experiment station peanut continuous cropping long term experiment in peanut florescence
Take 5 healthy plants, remove root bulky grain soil block, rhizosphere soil is acquired using dithering, takes back experiment after packing respectively
Room, 4 DEG C save backup.
Seed culture medium (LB): beef extract 5g, peptone 10g, sodium chloride 5g add deionized water to be settled to 1L, pH
7.2~7.4；
Minimal medium (MS): KH2PO43g, Na2HPO45g, NaCl 0.001g, CaCl2·H2O 0.003g,
MgSO4·5H2O 0.003g adds deionized water to be settled to 1L, pH 7.0.
Selective agar medium: the benzoic acid that 0.22 μm of filtering with microporous membrane is added by concentration requirement on minimal medium is female
Liquid (10gL-1)。
2% agar need to be added in the above solid medium, through 121 DEG C, 20min high pressure sterilization it is spare.
Data analysis: it is mapped using Excel 2016；SPSS 13.0 carries out data analysis, and it is poor that one way ANOVA is carried out
Different significance analysis.
Embodiment 1
The screening of degradation bacteria:
The screening of degradation bacteria uses the acclimation method that concentration of benzoic acid is gradually increased using benzoic acid as sole carbon source.Specifically
It operates as follows: accurately weighing 10g soil sample, be added in the triangular flask equipped with 90ml sterile water, fullyd shake in constant-temperature table
The soil suspension of 1:10 concentration is made in 30min.After grogs precipitating, 1ml supernatant is drawn, moves into and 9ml sterile water is housed
Test tube in, be made 10-2Bacteria suspension, and so on, it is made 10-3、10-4、10-5、10-6、10-7Bacteria suspension.Selection 10-4、10-5、10-6、10-7Each 100 μ l of four concentration, is added in benzoic acid Selective agar medium, concentration of benzoic acid by 5,10,15,
20mg·L-1Successively improve gradient domestication and enrichment degradation bacteria.By culture of repeatedly transferring, gradient dilution culture solution is simultaneously coated on
20 mg·L-1On benzoic acid solid selection medium, culture 48h is inverted in 30 DEG C of constant incubators.Picking colony form is not
Same single colonie is seeded on LB solid medium, and scribing line isolates and purifies to obtain pure bacterial strain repeatedly, and bacterial strain is enriched with through test tube slant
It is stored in after culture spare in 4 DEG C of refrigerators.
Degradation bacteria primary dcreening operation the results are shown in Table 1, using benzoic acid as sole carbon source, by 5,10,15,20mgL-1Concentration successively improves
Gradient domestication filters out 6 plants of degradation bacterias with enrichment altogether.Wherein degradation bacteria HJ-2 and HJ-3 biomass are maximum, and OD value respectively reaches
0.88 and 0.76, the degradation rate of para Toluic Acid reaches 96.88% and 92.65%.
The degradation rate of 1 different strains para Toluic Acid of table
Table 1 Degradation rate of Benzoic acid by different strains
Note: same column difference letter respectively indicates significant difference (P < 0.05) between processing.Notes:The different
letters in a column indicate significant differences among treatments at P<
0.05 levels.
Embodiment 2
The identification of degradation bacteria:
(1) degradation bacteria identification by morphological characters
Degradation bacteria observes the colony shape of degradation bacteria, size, color, transparency, sticky after LB culture medium culture 18h
Degree, wettability, protuberance and edge feature and whether chromogenic element etc., contaminated after thallus dyeing with ultramicroscopic observation thallus gram
The structures such as color, flagellum, pod membrane, gemma.Bacterium solution is after the fixed ethanol dehydration of glutaraldehyde and freeze-drying, scanning electron microscopic observation thallus
Size and surface texture.
(2) degradation bacteria physio-biochemical characteristics measure
Degradation bacteria carries out litmus milk, glucose, clark and Lubsreaction, V-P after LB culture medium culture 18h, according to document
Test, citrate, Starch Hydrolysis, gelatin liquefaction, catalase, oxidizing ferment, aerobism, generates H at indole reaction2S and nitrate
The physiological and biochemical tests such as reduction.
Test result shows: degradation bacteria HJ-2 is in yellow on LB culture medium, and protrusion, outer rim rounding, translucent, surface is wet
Profit, Gram-negative, rod-short, size are (0.7-1.6 × 1.0-1.5 μm) (Fig. 1), and degradation bacteria HJ-3 is on LB culture medium
White, translucent, edge is irregular, and surface is smooth, and Gram-negative is rod-shaped, and size is (0.5-1 × 1.5-4 μm) (Fig. 1)；
Degradation bacteria HJ-2 energy glucose response produces acid but do not produce gas, and can hydrolyze starch, liquefaction gelatin, contact enzyme positive, aerobic, degradation bacteria
HJ-3 catalase test, oxidase test, nitrate reduction test, citrate test are positive (table 2).
2 degradation bacteria HJ-2 and HJ-3 form of table and physiological and biochemical property
Note: "+" is the positive, and "-" is feminine gender
Note:"+",Positive；"–",Negative；
(3) measurement of degradation bacteria 16S rDNA gene order and the building of Molecular Phylogeny tree
Degradation bacteria genomic DNA extracts the total DNA of bacterial strain using (rapidly extracting box) kit, is drawn with 16S rDNA is general
Object 27F (forward primer): 5 '-AGAGTTTGATCCTGGCTCAG-3 '；1492 R (reverse primer): 5 '-
TACGGGTACCTTGTTACGACTT-3 ', the total DNA to separate degradation bacteria carry out PCR amplification as template.PCR reaction system (50
μ L): 1 μ L, 10 × PCR Buffer of DNA profiling 5 μ L, dNTP (2.5mmol/L) 4 μ L, primer (10 μm of ol/L) each 1 μ L, Tap
Enzyme (5U/ μ L) 0.5 μ L, 37.5 μ L of distilled water.Amplification program: 95 DEG C of 5min, 94 DEG C of 45s, 50 DEG C of 45s, 72 DEG C of 1min 15s；
32 circulations, 72 DEG C of 10min；After the reaction was completed, through 1% agarose electrophoresis, the size and specificity of amplified fragments are detected.PCR
Product is detected through agarose electrophoresis and is purified, and is served the raw work in sea and is carried out bidirectional sequencing and splice output complete sequence, 16S rDNA sequence
Sequence analysis is carried out with the 16S rDNA sequence included in Genebank, sequences match is carried out using ClustalX 1.8
Analysis uses adjacent method (Neighbor-Joiningmethod) phylogenetic tree construction by 6.0 software of MEGA, utilizes
Bootstrap (1000 repetitions) examines the confidence level of each branch.
The extraction that genomic DNA is carried out to degradation bacteria, and completes 16S rDNA gene sequencing, by obtained sequence with
Known array is compared in GenBank, and distinguishes phylogenetic tree construction, knot to two kinds of unknown degradation bacteria HJ-2 and HJ-3
Fruit is as shown in Figure 2.Show degradation bacteria HJ-2 and Achromobacter xylosoxidans according to the comparison of 16S rDNA sequence homology
(Achromobacter xylosoxidans) homology is nearest, and sequence similarity is up to 100%；HJ-3 then restores with nitro false
Monad (Pseudomonas nitroreducens) homology is nearest, and sequence similarity is up to 99%.In conjunction with the master of bacterium
Morphological feature and physiological and biochemical property are wanted, degradation bacteria HJ-2 and HJ-3 survey sequence results and be submitted to GenBank database, obtain
Sequence accession number is respectively MH324393 and MH324395.
Embodiment 3
Influence of the environmental factor to degradation bacteria degradation characteristic
In order to study the degradation situation of degradation bacteria HJ-2 and HJ-3 para Toluic Acid under the conditions of varying environment, select initial pH,
3 temperature, NaCl concentration influence factors carry out experiment of single factor as research object.
(1) initial pH is cultivated
Degradation bacteria is inoculated by 0.1% (OD600nm=0.8) inoculum concentration containing 20mgL-1Benzoic acid, pH be respectively 4,5,
6, in 7,8,9,10 inorganic salts culture solution, 30 DEG C, 180rmin-1Thalli growth amount and benzene first are measured after shaken cultivation 48h
The degradation rate of acid, thalli growth amount are 3 repetitions of every processing not connect degradation bacterium culture medium and compare.600nm after measurement processing
Locate absorbance value, the degradation rate of benzoic acid is the percentage of concentration of benzoic acid difference and starting concentration of benzoic acid before and after the processing.
(2) cultivation temperature
Degradation bacteria is inoculated into pH 7.0 by 0.1% (OD600nm=0.8) inoculum concentration, containing 20mgL-1Benzoic acid it is inorganic
In salt culture medium, control cultivation temperature is 25,30,35,40,45 DEG C, 180rmin-1Thalli growth is measured after shaken cultivation 48h
The degradation rate of amount and benzoic acid, thalli growth amount are 3 repetitions of every processing not connect degradation bacterium culture medium and compare.
(3) NaCl concentration
Degradation bacteria is inoculated into pH 7.0 by 0.1% (OD600nm=0.8) inoculum concentration, containing 20mgL-1Benzoic acid it is inorganic
In salt culture medium, control NaCl concentration is 0,2,4,6,8,10mgkg-1, cultivation temperature is 30 DEG C, 180rmin-1Oscillation
It cultivates, the degradation rate of thalli growth amount and benzoic acid is measured after 48h, thalli growth amount is not connect degradation bacterium culture medium and oppose
According to 3 repetitions of every processing.
As shown in figure 3, difference pH has an impact to 2 plants of degradation bacterias, the optimum pH value of degradation bacteria HJ-2 is experimental result
Between 6.0~9.0, and the optimum pH value of degradation bacteria HJ-3 is between 6.0~8.0, and pH is too high or too low all can be to two plants of bacterium
It generates and inhibits (Fig. 3 A).By Fig. 3 B it is found that degradation bacteria HJ-2 suitable growth temperature range is wider, grown at 30~45 DEG C good
It is good；Degradation bacteria HJ-3 suitable growth temperature is 30~35 DEG C, does not tolerate high temperature.Degradation bacteria HJ-3 to the variation of NaCl degree more
Sensitive (Fig. 3 C), when NaCl concentration is greater than 4%, growth is suppressed, and it is 0~4% that optimum, which grows NaCl concentration, but
Degradation bacteria HJ-2 tolerance NaCl ability is more stronger, and suitable NaCl concentration is 0~6%.In short, degradation bacteria HJ-2 is to adverse circumstance
Tolerance is strong compared with degradation bacteria HJ-3.
Embodiment 4
Benzoic acid, acetophenone, stearic acid, palmitinic acid, lactic acid, 3,5- bis- are extracted with 1: 1 ethanol ether hybrid extraction liquid
Tolyl aldehyde and glycerine culture solution 3 times before and after the processing, combining extraction liquid, rotary evaporation, esterification use n-hexane after cooling
Extraction, detects its degradation rate with gas chromatograph.Gas chromatography (GC) determination condition: GC is analyzed in Agilent gas-chromatography
It is carried out on instrument, using fid detector.GC condition: column model HP-FFAP, specification 30m × 0.32mm × 0.25 μm do not shunt,
Carrier gas N2(99%), flow 14.0mlmin-1, 220 DEG C of column temperature, 10 DEG C of min of temperature programming-1, and kept at 220 DEG C
10min, 260 DEG C of injector temperature, 260 DEG C of detector temperature, 1 μ l of sample volume.3 repetitions of every processing.
Degradation bacteria is inoculated by 0.1% (OD600nm=0.8) inoculum concentration with acetophenone, stearic acid, palmitinic acid, lactic acid, 3,
The allelochemicals such as 5- dimethylbenzaldehyde and glycerine are (20 mgL in the minimal medium of sole carbon source-1), culture temperature
Degree is 30 DEG C, 180rmin-1Measure thalli growth amount and Allelochemical degradation rate after shaken cultivation 48h, thalli growth amount be with
It does not connect degradation bacterium culture medium to compare, 3 repetitions of every processing.
In order to study whether the degradation bacteria of screening has degradation to other allelochemicals, the benzene second of identification early period is utilized
Ketone, stearic acid, 3,5- dimethylbenzaldehyde, palmitinic acid and lactic acid and glycerine etc. 6 cultivate peanut allelochemical carry out Degrading experiment,
It the results are shown in Table 3.In the inorganic salts using allelochemical as sole carbon source, degradation bacteria HJ-2 in addition to glycerine growth it is poor other than,
Other 5 kinds of allelochemicals can effectively degrade；Degradation bacteria HJ-3 is grown preferably using stearic acid, palmitinic acid as sole carbon source, and
It is most weak to being grown in lactic acid and glycerine.It can be seen that this two plants of degradation bacterias can use other allelochemicals as carbon source life
It is long, the degradation function with wide spectrum.
Degradation property of the 3 two plants of degradation bacterias of table to different allelochemicals
Table 3 Degradation rate of strain HJ-2 and strain HJ-3
Claims (9)
1. the degradation bacteria of allelochemical in a kind of degrading plant root exudates, which is characterized in that classification naming is xylose oxidation
Achromobacter (Achromobacter xylosoxidans), it is commonly micro- to be preserved in China Committee for Culture Collection of Microorganisms
Bio-Centers, the deposit date is on August 10th, 2017, culture presevation number was CGMCC NO.14517.
2. the degradation bacteria of allelochemical in a kind of degrading plant root exudates, which is characterized in that classification naming is nitro reduction
Pseudomonad (Pseudomonas nitroreducens), it is commonly micro- to be preserved in China Committee for Culture Collection of Microorganisms
Bio-Centers, the deposit date is on August 10th, 2017, culture presevation number was CGMCC NO.14518.
3. application of the degradation bacteria of any of claims 1 or 2 in degrading plant allelochemical.
4. degradation bacteria of any of claims 1 or 2 is alleviating the application in peanut continuous cropping obstacle.
5. application according to claim 3 or 4, which is characterized in that there is the allelochemical phenyl ring class or long-chain to be saturated
Fatty acid.
6. application according to claim 3 or 4, which is characterized in that the allelochemical is acetophenone, stearic acid, 3,5-
Dimethylbenzaldehyde or palmitinic acid.
7. a kind of method for allelochemical of degrading, which is characterized in that use degradation bacteria described in claim 1, degradation condition are as follows:
PH6.0~9.0,30~45 DEG C of temperature, NaCl concentration 0~6%.
8. a kind of method for allelochemical of degrading, which is characterized in that use degradation bacteria of any of claims 1 or 2, degradation condition
Are as follows: pH6.0~8.0,30~35 DEG C of temperature, NaCl concentration 0~4%.
9. a kind of microbial inoculum for allelochemical of degrading, which is characterized in that the microbial inoculum includes degradation bacteria of any of claims 1 or 2.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110699279A (en) * | 2019-04-15 | 2020-01-17 | 中国农业大学 | Achromobacter and application thereof in improving peanut iron nutrition |
| CN111480531A (en) * | 2020-05-26 | 2020-08-04 | 湖南省蔬菜研究所 | Method for relieving continuous cropping obstacle of watermelons |
| CN111979147A (en) * | 2019-06-17 | 2020-11-24 | 扬州大学 | Degrading bacterium for degrading allelochemicals in plant root exudates and application thereof |
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| CN111979147A (en) * | 2019-06-17 | 2020-11-24 | 扬州大学 | Degrading bacterium for degrading allelochemicals in plant root exudates and application thereof |
| CN111979147B (en) * | 2019-06-17 | 2021-11-23 | 扬州大学 | Degrading bacterium for degrading allelochemicals in plant root exudates and application thereof |
| CN111480531A (en) * | 2020-05-26 | 2020-08-04 | 湖南省蔬菜研究所 | Method for relieving continuous cropping obstacle of watermelons |
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| CN110283743B (en) | 2020-10-09 |
| CN111979147B (en) | 2021-11-23 |
| CN111979147A (en) | 2020-11-24 |
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