CN110282734A - A method of biological denitrificaion carbon source is prepared using containing sugared waste liquid - Google Patents
A method of biological denitrificaion carbon source is prepared using containing sugared waste liquid Download PDFInfo
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- CN110282734A CN110282734A CN201910606501.7A CN201910606501A CN110282734A CN 110282734 A CN110282734 A CN 110282734A CN 201910606501 A CN201910606501 A CN 201910606501A CN 110282734 A CN110282734 A CN 110282734A
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- waste liquid
- carbon source
- saccharomyces cerevisiae
- sugared
- carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The present invention provides a kind of method for utilizing and preparing biological denitrificaion carbon source containing sugared waste liquid, the method of the present invention includes three preparations of bio-carrier, the fixation of saccharomyces cerevisiae and the preparation of biological denitrificaion carbon source steps, the bio-carrier that the present invention uses is orange peel, orange peel nutriment abundant, breed the S. cervisiae being fixed thereon in fermenting carbohydrate substance, increase fermenting speed, sugared high conversion rate；Furthermore, orange peel is degraded during the fermentation, increase tunning amount, utilize S. cervisiae fermentation sugaring waste material, glucide in waste material is converted to low molecular pure and mild organic acid, compared with sugaring waste material directly makees denitrifying carbon source, effective component content is high, can being directly used as carbon source institute to avoid carbohydrate again, there is imitate the problems such as substance is few, foam is more, the present invention can make sugaring waste material, orange peel obtain higher value application, and conversion of the glucide to low mass molecule alcohol and organic acid is realized in the storage of product, transportational process.
Description
Technical field
The present invention is solid waste comprehensive utilization and technical field of biological treatment of wastewater, and in particular to a kind of using containing sugar
The method that waste liquid prepares biological denitrificaion carbon source.
Background technique
City domestic sewage and discharged volume of industrial waste water and Eutrophication materials increase, and lead to lake, Reservoir Eutrophication
It gets worse.And nitrogen, phosphorus substance are the restrictive factors of water body planktonic algae biological growth breeding, for this purpose, country is to dirty (useless)
The content of nitrogen and phosphorous has formulated stringent standard in water process water outlet.Biological denitrification denitrogenation is the main of dirty (useless) water process denitrogenation
Technique.
Denitrification denitrogenation is that denitrifying bacteria restores nitrate under anoxic conditions, releases molecular nitrogen (N2) or an oxygen
Change phenodiazine (N2O process), denitrifying bacterium is using various organic matters as electron donor in reaction process, using nitrate as electricity
Sub- receptor and carry out anaerobic respiration, denitrifying bacterium living environment must assure that suitable carbon-nitrogen ratio and suitable growth factor
And microelement.But in actual wastewater biochemical treatment process, since denitrification leading portion has been removed most of organic matter, cause
Keep wastewater carbon nitrogen out of proportion.So biological denitrificaion usually wants additional carbon in actual production.The waste water relatively low to C/N value,
Such as coking wastewater, refuse leachate, influence of the type and dosage of additional carbon to nitric efficiency are even more important.
Currently, common additional carbon mainly has: the molecular weight of methanol, ethyl alcohol, acetic acid, glucose etc., carbon source is lower, takes off
Nitrogen effect is better, but cost is relatively higher.So researching and developing the high substitution business carbon source of cost performance is the important of solution Denitrogenation
Research direction.
Chinese patent 200910076445.7 discloses a kind of using polylactic acid as carbon source and bio-carrier, this class set carbon
In multifunctional integrated material, price is higher for source, carrier, compares the life of the surface water for being suitable for low concentration TN, underground water, waste water
Object denitrogenation.A kind of open preparation method for proposing compounded carbons of Chinese patent 201811116149.0, utilizes sodium formate, acetic acid
Sodium, sodium propionate, glucide mix according to a certain percentage, and the problem of without reference to cheap carbon source is prepared using waste, China is specially
Benefit 201610352779 is open to propose that blackstrap is by slagging-off, resin using useless sugar as the method for sewage treatment denitrogenation carbon source
After decoloration, it is pumped in pond in anoxic section, the carbon source as biological denitrificaion.Although this application does carbon source using blackstrap, deposit
In following problems: being not carried out commercialization；The organic matter in blackstrap is not converted, to improve the effective use of carbon source
Rate.
So using containing sugared waste liquid preparation carbon source, there is also following technological gaps: 1) not converting waste, improve
The content of efficient carbon source component, to increase the efficiency of carbon source；2) commercialization of product is not accomplished.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of using the method for preparing biological denitrificaion carbon source containing sugared waste liquid,
This method not only realizes the recycling containing sugared waste liquid, and can be with commercialization of production product.Small point in denitrogenation carbon source obtained
Son amount organic acid, alcohol content are high, and nitric efficiency is high when use, and the specific implementation that the present invention invents is as follows:
The purpose of the present invention is to provide a kind of using the method for preparing biological denitrificaion carbon source containing sugared waste liquid, and technical point exists
In: specific step is as follows for the preparation of the biological denitrificaion carbon source:
S1. the preparation of bio-carrier: taking orange peel to be cut into strip, it sterilizes at high temperature then and is prepared into
Bio-carrier is stand-by；
S2. the fixation of saccharomyces cerevisiae: taking dried yeast powder to be inoculated in the conical flask for filling saccharomyces cerevisiae culture solution, then will
The bio-carrier prepared in S1 is also added in conical flask, and 5~8h is cultivated at 25~35 DEG C, after culture, solid-liquid point
From the saccharomyces cerevisiae that must be fixed in bio-carrier；
S3. the preparation of biological denitrificaion carbon source: taking containing sugared waste liquid, and adjusting is described to contain sugared brix and pH containing sugared waste liquid, then
The saccharomyces cerevisiae being fixed in bio-carrier that S2 is prepared is inoculated into containing in sugared waste liquid, then will be contained and is fixed on biological load
Being stored in container containing sugared waste liquid for saccharomyces cerevisiae in body is fermented, after fermentation up to biological denitrificaion carbon source.
In the embodiment having of the invention, the orange peel in the step S1 is the orange peel or fresh of drying regime
The length of orange peel, the orange peel shearing is 5~10mm, and the temperature of the orange peel sterilizing is 110~130 DEG C, institute
The time for the orange peel sterilizing stated is 10~20min.
In the embodiment having of the invention, every 1L saccharomyces cerevisiae culture solution is by following components system in the step S2
At:
Said components are mixed to the pH for adjusting saccharomyces cerevisiae culture solution with the sulfuric acid solution that volumetric concentration is 8~12% afterwards
Value is 4.5~5, is sterilized to obtain the saccharomyces cerevisiae culture later to saccharomyces cerevisiae culture solution with autoclaving
Liquid, the sterilising temp of the autoclaving are 110~130 DEG C, the sterilization time of the autoclaving
For 10~20min.
In the embodiment having of the invention, the weight of the weight of the step S2 dried yeast powder, saccharomyces cerevisiae culture solution
The weight ratio of amount and bio-carrier is 10~20:100~200:800, the saccharomyces cerevisiae culture solution after culture
Sugared concentration is 0.3~0.5wt.%.
In the embodiment having of the invention, the brix containing sugar containing sugared waste liquid is 10~40 ° after the step S3 is adjusted
Bx, the pH containing sugared waste liquid are 3~5.
In the embodiment having of the invention, the adjusting method containing sugared brix containing sugared waste liquid are as follows: toward the Fiao containing sugar
The middle distilled water that is added dropwise adjusts the brix containing sugar containing sugared waste liquid as 10~40 ° of Bx, the adjusting method of the pH containing sugared waste liquid are as follows: toward containing sugar
It is 3~5 that the sulfuric acid solution that volumetric concentration is 8~12% is added dropwise in waste liquid and adjusts the pH containing sugared waste liquid.
In the embodiment having of the invention, the weight of the saccharomyces cerevisiae being fixed in bio-carrier in the step S3
Measuring the ratio between (g) and the volume (L) containing sugared waste liquid is 2~10:100.
In the embodiment having of the invention, in the step S3 be inoculated with after the storage temperature containing sugared waste liquid be 15~
40 DEG C, the storage time containing sugared waste liquid after the inoculation is 5~15d, and the storage solutions containing sugared waste liquid are provided with
Gas vent periodically carries out artificial steam discharge, and the sugared conversion ratio containing sugared waste liquid after fermentation is 80~90wt.%.
Compared with prior art, the invention has the benefit that
1. a kind of utilize in the method for preparing biological denitrificaion carbon source containing sugared waste liquid provided by the invention is given birth to using orange peel
Object carrier obtains the S. cervisiae being fixed thereon in fermenting carbohydrate substance using orange peel nutriment abundant
Breeding increases fermenting speed, sugared high conversion rate；In addition, orange peel is degraded during the fermentation, increase tunning amount.
2. a kind of utilize provided by the invention utilizes S. cervisiae in the method for preparing biological denitrificaion carbon source containing sugared waste liquid
Fermentation sugaring waste material, is converted to low molecular pure and mild organic acid for the glucide in waste material, directly makees anti-nitre with sugaring waste material
Change carbon source to compare, effective component content is high, and can be directly used as carbon source institute to avoid carbohydrate there is effect substances that few, foam is more
The problems such as.
3. it is provided by the invention it is a kind of using prepared containing sugared waste liquid can make in the method for biological denitrificaion carbon source refine sugar waste material,
Orange peel obtains higher value application, and realizes glucide to low mass molecule alcohol and organic acid in the storage of product, transportational process
Conversion.
Specific implementation method
The technical scheme in the embodiments of the invention will be clearly and completely described below, so that the technology of this field
Personnel can better understand advantages and features of the invention, to make apparent boundary to protection scope of the present invention
It is fixed.Embodiment described in the invention is only a part of the embodiment of the present invention, instead of all the embodiments, based on the present invention
In embodiment, those of ordinary skill in the art's every other implementation obtained without making creative work
Example, shall fall within the protection scope of the present invention.
In the examples below, the nutriment analysis of components result of orange peel (every 100g dry weight) see the table below 1.
The nutriment composition of 1 orange peel of table (every 100g dry weight)
Embodiment 1
1. the preparation method of saccharomyces cerevisiae culture solution
Every 1L saccharomyces cerevisiae culture solution is made of the following components:
Said components are mixed to the pH value for adjusting saccharomyces cerevisiae culture solution with the sulfuric acid solution that volumetric concentration is 10% afterwards
Be 4.8, later with autoclaving to saccharomyces cerevisiae culture solution at a temperature of 120 DEG C, sterilizing 15min sterilized to obtain
The saccharomyces cerevisiae culture solution is spare.
2. utilizing the method for preparing biological denitrificaion carbon source containing sugared waste liquid
S1. the preparation of bio-carrier: taking the orange peel of drying regime or fresh orange peel to be cut into length is 7.5mm
Strip, it is stand-by that 15min that then it sterilizes at a temperature of 120 DEG C is prepared into bio-carrier for use；
S2. the fixation of saccharomyces cerevisiae: the dried yeast powder of 16 parts by weight is taken to be inoculated in the saccharomyces cerevisiae training for filling 200 parts by weight
In the conical flask of nutrient solution, then the bio-carrier of 800 parts by weight prepared in S1 is also added in conical flask, at 30 DEG C
6.5h is cultivated, after culture, solid- liquid separation obtains the saccharomyces cerevisiae being fixed in bio-carrier that sugared concentration is 0.4wt.%；
S3. the preparation of biological denitrificaion carbon source: taking the waste liquid containing sugar of 1000L, contains toward the Fiao containing sugar also middle dropwise addition distilled water adjusting
Sugared waste liquid is 40 ° of Bx containing sugared brix, is adjusted toward containing the sulfuric acid solution that dropwise addition volumetric concentration is 10% in sugared waste liquid containing sugared waste liquid
PH be 4, then the saccharomyces cerevisiae being fixed in bio-carrier that weight that S2 is prepared is 100g is inoculated into containing sugared waste liquid
In, then by being stored in container containing sugared waste liquid containing the saccharomyces cerevisiae being fixed in bio-carrier, ferment at 40 DEG C
15d, the biological denitrificaion carbon source for being after fermentation 90wt.% up to sugared conversion ratio.
Embodiment 2
1. the preparation method of saccharomyces cerevisiae culture solution
Every 1L saccharomyces cerevisiae culture solution is made of the following components:
It is by the pH value that said components mix the sulfuric acid solution for being afterwards 8% with volumetric concentration adjustment saccharomyces cerevisiae culture solution
4.5, later with autoclaving to saccharomyces cerevisiae culture solution at a temperature of 110 DEG C, sterilizing 20min sterilized to obtain institute
The saccharomyces cerevisiae culture solution stated is spare.
2. utilizing the method for preparing biological denitrificaion carbon source containing sugared waste liquid
S1. the preparation of bio-carrier: taking the orange peel of drying regime or fresh orange peel to be cut into length is 10mm
Strip, it is stand-by that 20min that then it sterilizes at a temperature of 110 DEG C is prepared into bio-carrier for use；
S2. the fixation of saccharomyces cerevisiae: the dried yeast powder of 16 parts by weight is taken to be inoculated in the saccharomyces cerevisiae training for filling 200 parts by weight
In the conical flask of nutrient solution, then the bio-carrier of 800 parts by weight prepared in S1 is also added in conical flask, at 25 DEG C
8h is cultivated, after culture, solid- liquid separation obtains the saccharomyces cerevisiae being fixed in bio-carrier that sugared concentration is 0.3wt.%；
S3. the preparation of biological denitrificaion carbon source: taking the waste liquid containing sugar of 1000L, contains toward the Fiao containing sugar also middle dropwise addition distilled water adjusting
Sugared waste liquid is 30 ° of Bx containing sugared brix, is adjusted toward containing the sulfuric acid solution that dropwise addition volumetric concentration is 8% in sugared waste liquid containing sugared waste liquid
PH is 3, and then the saccharomyces cerevisiae being fixed in bio-carrier that the weight that S2 is prepared is 800g is inoculated into the waste liquid containing sugar,
Then by being stored in container containing sugared waste liquid containing the saccharomyces cerevisiae being fixed in bio-carrier, ferment 12d at 37 DEG C, hair
The biological denitrificaion carbon source for being 90wt.% up to sugared conversion ratio after ferment.
Embodiment 3
1. the preparation method of saccharomyces cerevisiae culture solution
Every 1L saccharomyces cerevisiae culture solution is made of the following components:
Said components are mixed to the pH value for adjusting saccharomyces cerevisiae culture solution with the sulfuric acid solution that volumetric concentration is 12% afterwards
Be 5, later with autoclaving to saccharomyces cerevisiae culture solution at a temperature of 130 DEG C, sterilizing 10min sterilized to obtain institute
The saccharomyces cerevisiae culture solution stated is spare.
2. utilizing the method for preparing biological denitrificaion carbon source containing sugared waste liquid
S1. the preparation of bio-carrier: taking the orange peel of drying regime or fresh orange peel to be cut into length is 5mm's
Strip, it is stand-by that 10min that then it sterilizes at a temperature of 130 DEG C is prepared into bio-carrier for use；
S2. the fixation of saccharomyces cerevisiae: the dried yeast powder of 10 parts by weight is taken to be inoculated in the saccharomyces cerevisiae training for filling 100 parts by weight
In the conical flask of nutrient solution, then the bio-carrier of 800 parts by weight prepared in S1 is also added in conical flask, at 35 DEG C
5h is cultivated, after culture, solid- liquid separation obtains the saccharomyces cerevisiae being fixed in bio-carrier that sugared concentration is 0.5wt.%；
S3. the preparation of biological denitrificaion carbon source: taking the waste liquid containing sugar of 1000L, contains toward the Fiao containing sugar also middle dropwise addition distilled water adjusting
Sugared waste liquid is 25 ° of Bx containing sugared brix, is adjusted toward containing the sulfuric acid solution that dropwise addition volumetric concentration is 12% in sugared waste liquid containing sugared waste liquid
PH be 5, then the saccharomyces cerevisiae being fixed in bio-carrier that weight that S2 is prepared is 60g is inoculated into containing sugared waste liquid
In, then by being stored in container containing sugared waste liquid containing the saccharomyces cerevisiae being fixed in bio-carrier, ferment at 30 DEG C
12d, the biological denitrificaion carbon source for being after fermentation 90wt.% up to sugared conversion ratio.
Embodiment 4
1. the preparation method of saccharomyces cerevisiae culture solution
Every 1L saccharomyces cerevisiae culture solution is made of the following components:
It is by the pH value that said components mix the sulfuric acid solution for being afterwards 9% with volumetric concentration adjustment saccharomyces cerevisiae culture solution
4.6, later with autoclaving to saccharomyces cerevisiae culture solution at a temperature of 115 DEG C, sterilizing 12min sterilized to obtain institute
The saccharomyces cerevisiae culture solution stated is spare.
2. utilizing the method for preparing biological denitrificaion carbon source containing sugared waste liquid
S1. the preparation of bio-carrier: taking the orange peel of drying regime or fresh orange peel to be cut into length is 6mm's
Strip, it is stand-by that 12min that then it sterilizes at a temperature of 115 DEG C is prepared into bio-carrier for use；
S2. the fixation of saccharomyces cerevisiae: the dried yeast powder of 20 parts by weight is taken to be inoculated in the saccharomyces cerevisiae training for filling 150 parts by weight
In the conical flask of nutrient solution, then the bio-carrier of 800 parts by weight prepared in S1 is also added in conical flask, at 28 DEG C
6h is cultivated, after culture, solid- liquid separation obtains the saccharomyces cerevisiae being fixed in bio-carrier that sugared concentration is 0.35wt.%；
S3. the preparation of biological denitrificaion carbon source: taking the waste liquid containing sugar of 1000L, contains toward the Fiao containing sugar also middle dropwise addition distilled water adjusting
Sugared waste liquid is 20 ° of Bx containing sugared brix, is adjusted toward containing the sulfuric acid solution that dropwise addition volumetric concentration is 9% in sugared waste liquid containing sugared waste liquid
PH is 3.5, and then the saccharomyces cerevisiae being fixed in bio-carrier that the weight that S2 is prepared is 50g is inoculated into containing sugared waste liquid
In, then by being stored in container containing sugared waste liquid containing the saccharomyces cerevisiae being fixed in bio-carrier, ferment at 37 DEG C
14d, the biological denitrificaion carbon source for being after fermentation 90wt.% up to sugared conversion ratio.
Embodiment 5
1. the preparation method of saccharomyces cerevisiae culture solution
Every 1L saccharomyces cerevisiae culture solution is made of the following components:
Said components are mixed to the pH value for adjusting saccharomyces cerevisiae culture solution with the sulfuric acid solution that volumetric concentration is 10% afterwards
Be 4.7, later with autoclaving to saccharomyces cerevisiae culture solution at a temperature of 116 DEG C, sterilizing 13min sterilized to obtain
The saccharomyces cerevisiae culture solution is spare.
2. utilizing the method for preparing biological denitrificaion carbon source containing sugared waste liquid
S1. the preparation of bio-carrier: taking the orange peel of drying regime or fresh orange peel to be cut into length is 7mm's
Strip, it is stand-by that 13min that then it sterilizes at a temperature of 116 DEG C is prepared into bio-carrier for use；
S2. the fixation of saccharomyces cerevisiae: the dried yeast powder of 12 parts by weight is taken to be inoculated in the saccharomyces cerevisiae training for filling 130 parts by weight
In the conical flask of nutrient solution, then the bio-carrier of 800 parts by weight prepared in S1 is also added in conical flask, at 27 DEG C
7h is cultivated, after culture, solid- liquid separation obtains the saccharomyces cerevisiae being fixed in bio-carrier that sugared concentration is 0.46wt.%；
S3. the preparation of biological denitrificaion carbon source: taking the waste liquid containing sugar of 1000L, contains toward the Fiao containing sugar also middle dropwise addition distilled water adjusting
Sugared waste liquid is 15 ° of Bx containing sugared brix, is adjusted toward containing the sulfuric acid solution that dropwise addition volumetric concentration is 10% in sugared waste liquid containing sugared waste liquid
PH be 3.6, then the saccharomyces cerevisiae being fixed in bio-carrier that weight that S2 is prepared is 80g is inoculated into containing sugared waste liquid
In, then by being stored in container containing sugared waste liquid containing the saccharomyces cerevisiae being fixed in bio-carrier, ferment at 15 DEG C
15d, the biological denitrificaion carbon source for being after fermentation 85wt.% up to sugared conversion ratio.
Embodiment 6
1. the preparation method of saccharomyces cerevisiae culture solution
Every 1L saccharomyces cerevisiae culture solution is made of the following components:
Said components are mixed to the pH value for adjusting saccharomyces cerevisiae culture solution with the sulfuric acid solution that volumetric concentration is 11% afterwards
Be 4.5, later with autoclaving to saccharomyces cerevisiae culture solution at a temperature of 125 DEG C, sterilizing 18min sterilized to obtain
The saccharomyces cerevisiae culture solution is spare.
2. utilizing the method for preparing biological denitrificaion carbon source containing sugared waste liquid
S1. the preparation of bio-carrier: taking the orange peel of drying regime or fresh orange peel to be cut into length is 8mm's
Strip, it is stand-by that 18min that then it sterilizes at a temperature of 125 DEG C is prepared into bio-carrier for use；
S2. the fixation of saccharomyces cerevisiae: the dried yeast powder of 13 parts by weight is taken to be inoculated in the saccharomyces cerevisiae training for filling 165 parts by weight
In the conical flask of nutrient solution, then the bio-carrier of 800 parts by weight prepared in S1 is also added in conical flask, at 33 DEG C
6.5h is cultivated, after culture, solid- liquid separation obtains the saccharomyces cerevisiae being fixed in bio-carrier that sugared concentration is 0.48wt.%；
S3. the preparation of biological denitrificaion carbon source: taking the waste liquid containing sugar of 1000L, contains toward the Fiao containing sugar also middle dropwise addition distilled water adjusting
Sugared waste liquid is 10 ° of Bx containing sugared brix, is adjusted toward containing the sulfuric acid solution that dropwise addition volumetric concentration is 11% in sugared waste liquid containing sugared waste liquid
PH be 4.5, then the saccharomyces cerevisiae being fixed in bio-carrier that weight that S2 is prepared is 20g is inoculated into containing sugared waste liquid
In, then by being stored in container containing sugared waste liquid containing the saccharomyces cerevisiae being fixed in bio-carrier, ferment at 37 DEG C
12d, the biological denitrificaion carbon source for being after fermentation 80wt.% up to sugared conversion ratio.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than the present invention is protected
The limitation of range is protected, although explaining in detail referring to preferred embodiment to the present invention, those skilled in the art are answered
Work as understanding, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the reality of technical solution of the present invention
Matter and range.
Claims (8)
1. a kind of utilize the method for preparing biological denitrificaion carbon source containing sugared waste liquid, it is characterised in that: the biological denitrificaion carbon source
Specific step is as follows for preparation:
S1. the preparation of bio-carrier: taking orange peel to be cut into strip, it sterilizes at high temperature then and is prepared into biology
Carrier is stand-by；
S2. the fixation of saccharomyces cerevisiae: taking dried yeast powder to be inoculated in the conical flask for filling saccharomyces cerevisiae culture solution, then will be in S1
The bio-carrier prepared is also added in conical flask, 5~8h is cultivated at 25~35 DEG C, after culture, solid- liquid separation is obtained
The saccharomyces cerevisiae being fixed in bio-carrier；
S3. the preparation of biological denitrificaion carbon source: taking containing sugared waste liquid, and adjusting is described to contain sugared brix and pH containing sugared waste liquid, then by S2
The saccharomyces cerevisiae being fixed in bio-carrier prepared is inoculated into containing in sugared waste liquid, then will be contained and is fixed in bio-carrier
Being stored in container containing sugared waste liquid for saccharomyces cerevisiae ferment, after fermentation up to biological denitrificaion carbon source.
2. the method that a kind of utilization according to claim 1 prepares biological denitrificaion carbon source containing sugared waste liquid, it is characterised in that: institute
The orange peel in step S1 stated is the orange peel or fresh orange peel of drying regime, and the length of the orange peel shearing is 5
The temperature of~10mm, the orange peel sterilizing are 110~130 DEG C, and the time of the orange peel sterilizing is 10~20min.
3. the method that a kind of utilization according to claim 1 prepares biological denitrificaion carbon source containing sugared waste liquid, it is characterised in that: institute
Every 1L saccharomyces cerevisiae culture solution is made of the following components in the step S2 stated: (RED sector need not limit range)
It is by the pH value that said components mix the sulfuric acid solution for being afterwards 8~12% with volumetric concentration adjustment saccharomyces cerevisiae culture solution
4.5~5, saccharomyces cerevisiae culture solution is sterilized with autoclaving to obtain the saccharomyces cerevisiae culture solution later,
The sterilising temp of the autoclaving is 110~130 DEG C, and the sterilization time of the autoclaving is 10
~20min.
4. the method that a kind of utilization according to claim 1 prepares biological denitrificaion carbon source containing sugared waste liquid, it is characterised in that: institute
The weight ratio of the weight for the step S2 dried yeast powder stated, the weight of saccharomyces cerevisiae culture solution and bio-carrier is 10~20:100
~200:800, the sugared concentration of the saccharomyces cerevisiae culture solution is 0.3~0.5wt.% after culture.
5. the method that a kind of utilization according to claim 1 prepares biological denitrificaion carbon source containing sugared waste liquid, it is characterised in that: institute
The brix containing sugar containing sugared waste liquid is 10~40 ° of Bx after the step S3 stated is adjusted, and the pH containing sugared waste liquid is 3~5.
6. the method that a kind of utilization according to claim 5 prepares biological denitrificaion carbon source containing sugared waste liquid, it is characterised in that: institute
State the adjusting method containing sugared brix containing sugared waste liquid are as follows: adjust the brix containing sugar containing sugared waste liquid toward containing the sugared Fiao also middle distilled water that is added dropwise
For 10~40 ° of Bx, the adjusting method of the pH containing sugared waste liquid are as follows: toward containing the sulfuric acid that dropwise addition volumetric concentration is 8~12% in sugared waste liquid
It is 3~5 that solution, which adjusts the pH containing sugared waste liquid,.
7. the method that a kind of utilization according to claim 1 prepares biological denitrificaion carbon source containing sugared waste liquid, it is characterised in that: institute
The ratio between the weight (g) for the saccharomyces cerevisiae being fixed in bio-carrier in step S3 stated and volume (L) containing sugared waste liquid for 2~
10:100.
8. the method that a kind of utilization according to claim 1 prepares biological denitrificaion carbon source containing sugared waste liquid, it is characterised in that: institute
The storage temperature containing sugared waste liquid after being inoculated in the step S3 stated is 15~40 DEG C, the storage containing sugared waste liquid after the inoculation
Time is 5~15d, and the storage solutions containing sugared waste liquid are provided with gas vent or periodically carry out artificial steam discharge, described
The sugared conversion ratio containing sugared waste liquid after fermentation is 80~90wt.%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112850912A (en) * | 2020-12-29 | 2021-05-28 | 南京尚迪纳米科技有限公司 | Biological composite carbon source and preparation method and application thereof |
| CN115057580A (en) * | 2022-06-07 | 2022-09-16 | 鞍钢集团工程技术有限公司 | Sewage denitrification carbon source prepared by chemical waste liquid resource utilization and preparation method and system thereof |
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