CN110257531A - A method of for detecting prey Dominant Natural Enemies - Google Patents
A method of for detecting prey Dominant Natural Enemies Download PDFInfo
- Publication number
- CN110257531A CN110257531A CN201910623509.4A CN201910623509A CN110257531A CN 110257531 A CN110257531 A CN 110257531A CN 201910623509 A CN201910623509 A CN 201910623509A CN 110257531 A CN110257531 A CN 110257531A
- Authority
- CN
- China
- Prior art keywords
- natural enemy
- prey
- life period
- digestion
- natural
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 33
- 230000029087 digestion Effects 0.000 claims abstract description 46
- 238000001514 detection method Methods 0.000 claims abstract description 12
- 210000003736 gastrointestinal content Anatomy 0.000 claims abstract description 9
- 238000012937 correction Methods 0.000 claims abstract description 6
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 32
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 32
- 235000018262 Arachis monticola Nutrition 0.000 claims description 32
- 235000020232 peanut Nutrition 0.000 claims description 32
- 241001124076 Aphididae Species 0.000 claims description 26
- 241000199695 Harmonia <beetle> Species 0.000 claims description 15
- 238000012408 PCR amplification Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 3
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- 241001553178 Arachis glabrata Species 0.000 claims 2
- 244000062645 predators Species 0.000 abstract description 15
- 241000607479 Yersinia pestis Species 0.000 abstract description 10
- 238000011002 quantification Methods 0.000 abstract description 3
- 230000009467 reduction Effects 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 244000105624 Arachis hypogaea Species 0.000 description 30
- 241000042120 Propylea japonica Species 0.000 description 10
- 241000498522 Aphis medicaginis Species 0.000 description 6
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 241000255749 Coccinellidae Species 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 238000000611 regression analysis Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 241000238421 Arthropoda Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000237852 Mollusca Species 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 244000062804 prey Species 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
- G06F17/10—Complex mathematical operations
- G06F17/18—Complex mathematical operations for evaluating statistical data, e.g. average values, frequency distributions, probability functions, regression analysis
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Data Mining & Analysis (AREA)
- General Physics & Mathematics (AREA)
- Theoretical Computer Science (AREA)
- Mathematical Optimization (AREA)
- Mathematical Analysis (AREA)
- Pure & Applied Mathematics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Mathematical Physics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Computational Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Genetics & Genomics (AREA)
- Evolutionary Biology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Operations Research (AREA)
- Probability & Statistics with Applications (AREA)
- Immunology (AREA)
- Algebra (AREA)
- Biotechnology (AREA)
- Databases & Information Systems (AREA)
- Software Systems (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to agricultural technology fields, and in particular to a method of for detecting prey Dominant Natural Enemies.This method carries out round pcr detection using the genome of the COI primer pair natural enemy sample of prey, determines that Dominant Natural Enemies, the predation are by the positive rate after digestion half-life period weighted value correction by comparing the predation of natural enemy；The preparation method of the digestion half-life period weighted value are as follows: prey calculates digestion half-life period in the positive rate of natural enemy intestinal content after counting different digestions time under experimental conditions；By the digestion half-life data of different natural enemies, the digestion half-life period weighted value of different natural enemies is calculated.Method in the present invention can use weighted value and be corrected to digestion rate, really reduction and relatively predation situation of a variety of predators in relative quantification field to prey, the Dominant Natural Enemies of prey pest under different times are determined, to correctly evaluate the Pest control efficacy of predator.
Description
Technical field
The present invention relates to agricultural technology fields, and in particular to a method of for detecting prey Dominant Natural Enemies.
Background technique
In farmland ecosystem, for injurious insect control, the Pest control efficacy of natural enemy accounts for 50% or more.In Farmland Landscape system
In system, the type and quantity of predator are very rich, play an important role in terms of control pest is caused harm, clearly prey on
Property Natural Enemies and various predators are to give full play to natural enemy control action to the size of the control action of peanut aphid
Basis and premise.With the development of molecular biology method, Recent study is more, utilizes based on DNA molecular
Alimentary canal content analyze the nutrition relationship between predator and prey is quantitatively evaluated.But due to different predators
Different to the digestion rate of prey, therefore, it is necessary to a kind of proper correction methods to be corrected to digestion rate, really reduction and phase
To quantitative field predator to the predation situation of prey, to correctly evaluate the Pest control efficacy of predator.
Summary of the invention
(1) technical problems to be solved
In order to solve the deficiencies in the prior art, the present invention provides one kind can really restore the simultaneously a variety of fields of relative quantification
Method of the predator to the predation situation of prey, to further determine that the Dominant Natural Enemies of prey.
(2) technical solution
A method of for detecting prey Dominant Natural Enemies, using prey COI primer pair natural enemy sample genome into
The detection of row round pcr determines that Dominant Natural Enemies, the predation are by digesting half-life period by comparing the predation of natural enemy
Positive rate after weighted value (Predator importance weighting value, W) correction；
The preparation method of the digestion half-life period weighted value are as follows: count prey in different digestions time under experimental conditions and exist
Positive rate in natural enemy intestinal content calculates digestion half-life period (DNAdetectability half-life, DDH)；
The smallest natural enemy weighted value of half-live values will be digested and be set as 1, Wi=DDHMin/DDHj, in formula, WiFor the digestion of natural enemy to be measured
Half-life period weighted value, DDHMinFor the digestion half-live values for digesting the smallest natural enemy of half-live values, DDHjIt is described to be measured
The digestion half-live values of natural enemy；
Preferably, the circular of the digestion half-life period is as follows:
Using digestion time as abscissa, positive rate obtains digestion half-life period map, passes through index as ordinate
Regression analysis calculates harmonia axyridia to the digestion half-life period of peanut aphid.
Preferably, detailed process is as follows: P for the correctioni=PPPj*Wi, in formula, PiMake for the predation of natural enemy to be measured
With (Prey predation, P), PPPjFor the positive rate to be corrected of natural enemy to be measured, WiFor the digestion half-life period of natural enemy to be measured
Weighted value.
Preferably, natural enemy insect sample collection method is as follows: taking emergence time consistent when prey is peanut aphid
Not in contact with the predator (every group of 240 adults, i.e., each 20 adults of time-triggered protocol repeat) of peanut aphid, single head is put into
35mm culture dish, Nature enemy 24 hours, feeding single head 2 age peanut aphid.T is calculated as at the time of predator feeding is finished
=0 (giving up the predator that non-feeding finishes in 2h), respectively at 0h, 1h, 2h, 3h, 4h, 6h, 8h, 12h, 16h, 20h,
For 24 hours, 48h samples ladybug, and sample is immersed in dehydrated alcohol, saves in -20 DEG C of refrigerators.
Preferably, the natural enemy sample is intestinal content or the enemy of the whole previous day of natural enemy, preferably in the enteron aisle of natural enemy
Inclusion.
Preferably, by kit (QIAGEN DNeasy Blood and Tissue Kit (QIAGEN Inc.,
Chatsworth, CA, USA)) or CTAB extraction method extract the genome of the natural enemy sample.
Preferably, when the prey is peanut aphid, COI primer sequence are as follows:
HS-F1:5′-GGAATAATTGGATCTTCACTTAGTATT-3′(SEQ ID No.1)；
HS-R1:5′-AAGGTAGTTCTGAATATGAATGTTCTA-3′(SEQ ID No.2)。
When stating COI primer sequence in use, preferably, in round pcr detection, the item of pcr amplification reaction
Part are as follows: 98 DEG C of 10s, 55 DEG C of 30s, 72 DEG C of 30s, after 30 recycle, 72 DEG C of 10min.
Preferably, in round pcr detection, the reaction system of pcr amplification reaction are as follows:
The present invention further provides the method technical field of biological control application.
(3) beneficial effect
Method in the present invention can be corrected digestion rate, really reduction and a variety of predatism in relative quantification field
The predation situation of its hostile prey, determines the Dominant Natural Enemies of prey pest under different times, to correctly evaluate predator
Pest control efficacy, accurately promote biological prevention with more efficient.
The very high COI primer of a species specificity especially is provided in the detection method for peanut aphid, into one
Step is convenient for clearly various predators to the size of the control action of peanut aphid, ecology of the enhancing natural enemy to Aphis medicaginis
Regulating and controlling effect achievees the purpose that pest controlling is increased production.
Detailed description of the invention
Fig. 1 is peanut aphid in the intracorporal digestion half-life period map of harmonia axyridia；
Fig. 2 is peanut aphid in the intracorporal digestion half-life period map of Propylaea japonica；
Fig. 3 is to be corrected positive rate statistics of the peanut aphid in Propylaea japonica and harmonia axyridia intestinal content；
Fig. 4 is different times Peanut Fields harmonia axyridia compared with Propylaea japonica is to peanut aphid predation；
Fig. 5 is using Aphis medicaginis genome as the primer amplification electrophoretogram of template, wherein M:marker, 1-2 are with Aphis medicaginis base
Because organizing the primer amplification electrophoretogram for template；
Fig. 6 is that primer specificity screens electrophoretogram, wherein M:marker, 1-127: with the master of generations all in Peanut Fields
It wants arthropod species and mollusk genome is the primer amplification electrophoretogram of template.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
The present embodiment provides the methods that one kind obtains natural enemy digestion half-life data under experimental conditions, specifically include as follows
Step:
(1) take emergence time consistent not in contact with Aphis medicaginis harmonia axyridia (every group of 240 adults, i.e., at each time
20 adults are managed to repeat), single head is put into 35mm culture dish, and Nature enemy 24 hours, feeding single head 2 age peanut aphid.By ladybug
T=0 (giving up the ladybug that non-feeding finishes in 2h) is calculated as at the time of feeding finishes, respectively at 0h, 1h, 2h, 3h, 4h, 6h, 8h,
12h, 16h, 20h, for 24 hours, 48h sample ladybug, and sample is immersed in dehydrated alcohol, save in -20 DEG C of refrigerators；
(2) it detects using round pcr and counts the positive in different digestion time section preys in natural enemy intestinal content
Recall rate, using digestion time as abscissa, recall rate obtains digestion half-life period map (see Fig. 1), passes through finger as ordinate
It is 3.284h (R to the digestion half-life period of peanut aphid that harmonia axyridia, which is calculated, in number regression analysis2=0.9575)；
The recall rate preparation method is as follows:
(2a) by kit (QIAGEN DNeasy Blood and Tissue Kit (QIAGEN Inc.,
Chatsworth, CA, USA)) DNA that whole head insect obtains harmonia axyridia sample is extracted, and by agarose gel electrophoresis to institute
Obtained genome quality is extracted to be verified；
(2b) carries out pcr amplification reaction using the DNA of primer pair harmonia axyridia sample, wherein primer sequence are as follows:
HS-F1:5′-GGAATAATTGGATCTTCACTTAGTATT-3′(SEQ ID No.1)；
HS-R1:5′-AAGGTAGTTCTGAATATGAATGTTCTA-3′(SEQ ID No.2)；
The reaction system of the pcr amplification reaction are as follows:
The condition of the pcr amplification reaction are as follows: 98 DEG C of 10s, 55 DEG C of 30s, 72 DEG C of 30s, after 30 recycle, 72 DEG C of 10min；
(2c) is verified by the product that agarose gel electrophoresis obtains amplified reaction, is counted and is occurred in amplified reaction
The number of specific amplification, obtains recall rate.
Embodiment 2
The present embodiment the difference from embodiment 1 is that: natural enemy to be detected be Propylaea japonica.
Obtained digestion half-life period map is shown in Fig. 2, and Propylaea japonica is calculated to Aphis medicaginis by exponential regression analysis method
The digestion half-life period of worm is 4.355h (R2=0.9337).
Embodiment 3
The present embodiment provides a kind of according to embodiment 1, the method for 2 Data Detection prey Dominant Natural Enemies, and detailed process is as follows:
The harmonia axyridia in field, Propylaea japonica are carried out in different times (June 10, June 20, July 10) respectively
Sampling, obtains peanut aphid in two kinds of natural enemy intestinal contents using method identical with recall rate preparation method in embodiment 1
In positive rate to be corrected (Fig. 3).
By the digestion half-live values in Examples 1 to 2, the digestion half-life period weighted value (table 1) of every kind of natural enemy is calculated.
The peanut aphid positive rate of harmonia axyridia, Propylaea japonica is corrected respectively using digestion half-life period weighted value, calculate,
Compare the two predation size, determines the Dominant Natural Enemies of different times peanut natural enemy.
Digestion half-life period and weight calculation of the 1 peanut aphid of table in two kinds of natural enemy intestinal contents
The calculation method of predation is as follows: Pi=PPPj*Wi, in formula, PiFor the predation (Prey of natural enemy to be measured
Predation, P), PPPjFor the positive rate to be corrected of natural enemy to be measured, WiFor the digestion half-life period weighted value of natural enemy to be measured.
Obtained predation result figure is shown in Fig. 4, as seen from the figure, harmonia axyridia and moire wooden dipper under Peanut Fields different times
Worm has notable difference to the predation of peanut aphid.By the comparison to its predation it is found that in June 10, June 20
Front and back, compared to Propylaea japonica, the predation of harmonia axyridia is significantly greater, is the Dominant Natural Enemies of peanut aphid；July 10
The predation of front and back, harmonia axyridia is decreased obviously, but still has biggish predation compared with Propylaea japonica, is still peanut
The Dominant Natural Enemies of aphid.
After the predation of the hostile Aphis medicaginis worm in two kinds of days is corrected by weighted value, relative to harmonia axyridia, Propylaea japonica
Predation have dropped 25%.Therefore, by this comparative example it is known that a variety of natural enemies when same prey exist simultaneously
When, the present invention can accurately trace the predation size of the hostile prey pest in day, specific conducive to being anchored in field produces
The Dominant Natural Enemies of pest, to realize being precisely controlled for ecological regulation and control.
Test example 1
This test example detects the specificity of the COI primer sequence of peanut aphid in the present invention.
Peanut aphid sample is detected first, in accordance with the round pcr detection method in embodiment 1, testing result is shown in
Fig. 5, as seen from the figure, the specific band that the primer in the present invention can be clear out, single for peanut aphid sequence amplification.
The present invention is further according to the method in embodiment 1, with the main arthropod species of generations all in Peanut Fields
And mollusk genome is template, detection primer specificity the results are shown in Table 2 and Fig. 6, by table 2 and Fig. 6 result it is found that this hair
Other species with peanut aphid in identical environment of primer pair in bright are without expanding effect.
From the foregoing, it will be observed that primer sequence has very high specificity in the present invention.
The species being related in the detection of 2 primer specificity of table
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Sequence table
<110>Shandong Peanut Inst.
<120>a kind of method for detecting prey Dominant Natural Enemies
<130> KHP191112768.6
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 27
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 1
ggaataattg gatcttcact tagtatt 27
<210> 2
<211> 27
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
aaggtagttc tgaatatgaa tgttcta 27
Claims (10)
1. a kind of method for detecting prey Dominant Natural Enemies is carried out using the genome of the COI primer pair natural enemy sample of prey
Round pcr detection, which is characterized in that determine that Dominant Natural Enemies, the predation are to pass through by comparing the predation of natural enemy
Positive rate after digesting the correction of half-life period weighted value；
The preparation method of the digestion half-life period weighted value are as follows: count in different digestions time prey under experimental conditions in natural enemy
Positive rate in intestinal content calculates digestion half-life period；The smallest natural enemy weighted value of half-live values will be digested to be set as
1, Wi=DDHMin/DDHj, in formula, WiFor the digestion half-life period weighted value of natural enemy to be measured, DDHMinIt is minimum for digestion half-live values
Natural enemy digestion half-live values, DDHjFor the digestion half-live values of the natural enemy to be measured.
2. the method according to claim 1, wherein the circular of the digestion half-life period is as follows:
Using digestion time as abscissa, positive rate obtains digestion half-life period map, passes through index return as ordinate
Analytic approach calculates harmonia axyridia to the digestion half-life period of peanut aphid.
3. method according to claim 1 or 2, which is characterized in that detailed process is as follows for the correction: Pi=PPPj*
Wi, in formula, PiFor the predation of natural enemy to be measured, PPPjFor the positive rate to be corrected of natural enemy to be measured, WiFor natural enemy to be measured
Digest half-life period weighted value.
4. method described in any one of claim 1 to 3, which is characterized in that the natural enemy sample is the enteron aisle of natural enemy
Content or enemy of the whole previous day.
5. according to the method described in claim 4, it is characterized in that, the natural enemy sample is the intestinal content of natural enemy.
6. method according to any one of claims 1 to 5, which is characterized in that mentioned by kit or CTAB extraction method
Take the genome of the natural enemy sample.
7. the method according to claim 1, wherein the prey be peanut aphid when, COI primer sequence are as follows:
HS-F1:5'-GGAATAATTGGATCTTCACTTAGTATT-3'；
HS-R1:5'-AAGGTAGTTCTGAATATGAATGTTCTA-3'。
8. the method according to the description of claim 7 is characterized in that the round pcr detection in, the item of pcr amplification reaction
Part are as follows: 98 DEG C of 10s, 55 DEG C of 30s, 72 DEG C of 30s, after 30 recycle, 72 DEG C of 10min.
9. method according to claim 7 or 8, which is characterized in that in round pcr detection, pcr amplification reaction
Reaction system are as follows:
10. any one of claim 1~9 the method is in the application of technical field of biological control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910623509.4A CN110257531A (en) | 2019-07-11 | 2019-07-11 | A method of for detecting prey Dominant Natural Enemies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910623509.4A CN110257531A (en) | 2019-07-11 | 2019-07-11 | A method of for detecting prey Dominant Natural Enemies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110257531A true CN110257531A (en) | 2019-09-20 |
Family
ID=67925673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910623509.4A Pending CN110257531A (en) | 2019-07-11 | 2019-07-11 | A method of for detecting prey Dominant Natural Enemies |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110257531A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468677A (en) * | 2013-09-18 | 2013-12-25 | 中国农业科学院植物保护研究所 | Specific SS-COI (Species-specific Cytochrome Oxidase I) primers of aphis craccivora, kit containing primers and detecting method of kit |
| CN105567843A (en) * | 2016-02-04 | 2016-05-11 | 浙江大学 | Composite tag for rice field euryphagous natural predator prey diversity high-throughput sequencing and application thereof |
-
2019
- 2019-07-11 CN CN201910623509.4A patent/CN110257531A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468677A (en) * | 2013-09-18 | 2013-12-25 | 中国农业科学院植物保护研究所 | Specific SS-COI (Species-specific Cytochrome Oxidase I) primers of aphis craccivora, kit containing primers and detecting method of kit |
| CN105567843A (en) * | 2016-02-04 | 2016-05-11 | 浙江大学 | Composite tag for rice field euryphagous natural predator prey diversity high-throughput sequencing and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| JASON M SCHMIDT等: "Predator-prey trophic relationships in response to organic management practices", 《MOL ECOL》 * |
| QIAN JUA等: "Strip intercropping peanut with maize for peanut aphid biological control", 《AGRICULTURE, ECOSYSTEMS AND ENVIRONMENT》 * |
| 威海市地方史志编纂委员会编: "《威海市志》", 31 March 1983 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108949985B (en) | Circ-WHSC1 is as diagnosis of colorectal carcinoma marker and its application | |
| CN112980999B (en) | SSR molecular marker of mulberry variety Yuehei 74 and core primer group, kit and application thereof | |
| CN105969845B (en) | The molecular labeling of eye muscle area character related gene SVEP1 and its application | |
| CN102242121B (en) | Fast growth gene and parent screening method for micropterus salmoides | |
| CN104818334A (en) | Tiny RNA related to lung adenocarcinoma metastasis | |
| CN104073486A (en) | SNP site related to rapid growth of largemouth black bass as well as identification method and application thereof | |
| CN103361346A (en) | Method for cloning and analyzing populus diversifolia micro RNAs (ribonucleic acids) precursor | |
| CN110257531A (en) | A method of for detecting prey Dominant Natural Enemies | |
| CN103468798A (en) | Detecting method for yeast-like-symbiotes in vivo of brown planthopper in different instars | |
| CN110343769A (en) | A kind of COI primer and its application for peanut aphid PCR detection | |
| CN102424824B (en) | DNA (deoxyribonucleic acid) in-vivo sampling method for young Hyriopsis cumingii | |
| CN104531760B (en) | The short hairpin RNA interference plasmid and its application process of Dp71 albumen | |
| CN101974516B (en) | Method for discriminating Chinese milk vetch variety by using SSR (Simple Sequence Repeat)fingerprint spectrum | |
| CN104073557A (en) | Fluorescence quantitative reference genes for crassostrea gigas Poly (I:C) stress experiment as well as primers and applications of fluorescence quantitative reference genes | |
| CN110616279B (en) | Kit for synchronously and quantitatively detecting 3 important shrimp pathogens | |
| CN109811085B (en) | Primer for identifying purity of autumn-sown Chinese cabbage Zhenlun No. 6 seeds and application of primer | |
| CN112941218A (en) | Method for identifying authenticity of yam germplasm resources by virtue of cpPSSR molecular marker method | |
| CN101988127A (en) | Method for detecting peanut LOX (Lipoxygenase) gene mRNA expression level by real-time fluorescent quantitative PCR (Polymerase Chain Reaction) | |
| CN104789703B (en) | A method of by detecting plumule inner virus growth rate Fast Evaluation wheat rosette stunt varietal resistance | |
| CN101613741A (en) | Identify the novel method of Pacific oyster population | |
| CN101633936B (en) | Fish transgenosis breeding method | |
| Ren et al. | A novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host | |
| CN114561479B (en) | Primer for identifying iron-shell shrimp individuals in macrobrachium rosenbergii and application of primer | |
| CN106498027A (en) | A kind of anti-rough dwarf disease authentication method of Semen Maydiss | |
| CN107254532B (en) | Identification method of non-coding RNA (ribonucleic acid) related to spiroplasma infection, non-coding RNA and application of non-coding RNA |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190920 |
|
| RJ01 | Rejection of invention patent application after publication |