CN110257473A - Resistant rice varieties plant blast resisting identification method in specific rice region - Google Patents
Resistant rice varieties plant blast resisting identification method in specific rice region Download PDFInfo
- Publication number
- CN110257473A CN110257473A CN201910585583.1A CN201910585583A CN110257473A CN 110257473 A CN110257473 A CN 110257473A CN 201910585583 A CN201910585583 A CN 201910585583A CN 110257473 A CN110257473 A CN 110257473A
- Authority
- CN
- China
- Prior art keywords
- rice
- resistant
- resistance
- gene
- blast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000209094 Oryza Species 0.000 title claims abstract description 138
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 138
- 235000009566 rice Nutrition 0.000 title claims abstract description 138
- 238000000034 method Methods 0.000 title claims abstract description 18
- 241000196324 Embryophyta Species 0.000 title claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 66
- 231100000252 nontoxic Toxicity 0.000 claims abstract description 38
- 230000003000 nontoxic effect Effects 0.000 claims abstract description 38
- 238000004519 manufacturing process Methods 0.000 claims abstract description 24
- 241001330975 Magnaporthe oryzae Species 0.000 claims abstract description 20
- 238000011081 inoculation Methods 0.000 claims abstract description 9
- 230000007170 pathology Effects 0.000 claims abstract description 3
- 238000011160 research Methods 0.000 claims abstract description 3
- 238000004458 analytical method Methods 0.000 claims description 12
- 208000015220 Febrile disease Diseases 0.000 claims description 5
- 230000001932 seasonal effect Effects 0.000 claims description 5
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 4
- 238000012408 PCR amplification Methods 0.000 claims description 4
- 230000000680 avirulence Effects 0.000 claims description 4
- 241000700605 Viruses Species 0.000 claims description 2
- 230000008485 antagonism Effects 0.000 claims description 2
- 238000007405 data analysis Methods 0.000 claims description 2
- 238000012252 genetic analysis Methods 0.000 claims description 2
- 238000002955 isolation Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 238000000386 microscopy Methods 0.000 claims description 2
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 238000012797 qualification Methods 0.000 abstract description 11
- 201000004569 Blindness Diseases 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 abstract description 2
- 238000005507 spraying Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000008034 disappearance Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 208000002109 Argyria Diseases 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241001344131 Magnaporthe grisea Species 0.000 description 1
- 235000006041 Prunus persica f compressa Nutrition 0.000 description 1
- 240000006522 Prunus persica f. compressa Species 0.000 description 1
- 241000231139 Pyricularia Species 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 231100000749 chronicity Toxicity 0.000 description 1
- 230000010460 detection of virus Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of resistant rice varieties and plants blast resisting identification method in specific rice region, include the following steps, rely on molecular biology and Plant Pathology research means, know that rice varieties promote and apply the nontoxic gene type structure of the Pyricularia oryzae population of rice region first, next knows the key resistance genotype of the rice varieties of popularization and application, then two results are compared, and comprehensive inoculation is carried out to rice varieties using rice region flora, whether rice resistant variety is finally efficiently identified in the quasi- rice region performance resistance promoted and applied.The present invention passes through the Molecular Detection of resistant gene and nontoxic gene, such as corresponding to exist, then shows that the kind is resistant in Production of Large Fields；Exist if not corresponding, then further by spraying qualification result, distinguishes whether the kind is resistant in Production of Large Fields.The present invention is greatly reduced because blindness uses the loss in the production of resistant variety bring.
Description
Technical field
The invention belongs to rice varieties identification technology fields, and in particular to a kind of resistant rice varieties are planted in specific rice region
Blast resisting identification method.
Background technique
Rice is the important cereal crops in Hubei Province or even the whole nation, and rice blast is puzzlement Rice Production, interferes rice high-quality
One of important disease of high yield.Rice blast not only has a popular popularity, but also physiological races of rice blast fungus (pathological form)
Distribution can be different due to the difference of the weather conditions of different regions and tillage and cultivation mode, and there is also difference between year, because
And rice blast has complexity and chronicity in prevention and control.
The prevention and treatment most economical effective means of rice blast are to utilize resistant variety in production at present.Know whether certain kind is anti-
Property kind, i.e., to be identified by the spontaneous induction in strain's area experiment sick nursery garden, qualification result be divided into highly resistance, it is disease-resistant, in resist, it is middle sense,
Susceptible, high sense.Strain's area experiment sick nursery garden multiselect location is in low-lying mountain area, the long-term Rainy sparse sunlight in this area, mistiness dew weight, low temperature summer
Cool, Pyricularia oryzae population structure complexity, strong toxicity are Chang Faqu and the repeating transmission area of rice blast.Resist the above qualification result in having
Kind could promote and apply in production, however actually but occur area examination sick nursery garden identification resistant variety in Production of Large Fields
In it is not disease-resistant, to find out its cause, in addition to a certain resistant variety of the long-term single cropping of large area cause it is non-advantage in Pyricularia oryzae population
Pathological form rises to advantage pathological form, shows as resistance " forfeiture " outside so as to cause resistant variety, another important factor
It is exactly the nontoxic gene type that inherently has with the Pyricularia oryzae population of plantation rice region of the resistant gene that has of resistant variety not contract
It closes, in other words, the resistance majority of resistant variety does not have unifying property, does not follow Pyricularia oryzae-rice two in popularization and application
" gene-for-gene " relationship possessed by between person, because of resistant gene and the rice region Pyricularia oryzae population without considering resistant variety
The behavior whether nontoxic gene matches very likely will lead to that resistant variety is not disease-resistant, bring to the green safe production of rice
Risk.
Whether the rice material of Resistance Identification qualifications is obtained in other rice regions in area's pilot that rice blast often retransmits area
Production of Large Fields in " holding " resistance? actual production the result is that in some rice region resistant rice varieties " holding " resistances, and
In the resistance " disappearance " of the same resistant rice varieties of other rice region.The phenomenon that this resistance " disappearance ", substantially on obtain
Resistance qualifications the nontoxic gene type of resistant gene and the rice blast flora of place rice region that has of resistant variety it is not right
Should there are (Bacterial community of Bacterial community and place rice region because of area's pilot has significantly different), the resistance that resistant variety has
Gene is unable to normal expression in place rice region.
The prior art: the excavation and utilization of resistant variety usually rely on a variety of breeding techniques to obtain, and have 1 or multiple masters
Whether the kind for imitating resistant gene in field just shows disease resistance, and Resistant expression of the kind in field only passes through kind and have in other words
Some resistant genes just can determine whether that this is the problem of most rice breeding experts ignore.What they obtained is only in specific region
The interior resistant material for passing through Resistance Identification and obtaining proof.
How in a certain rice region resistant variety is efficiently utilized, there is an urgent need to a kind of efficiently identification resistant varieties in the rice region
Whether the technology of blast resisting.
Summary of the invention
The present invention is in the work with reliable and stable identification rice blast pathogenic type and kind to rice blast resistance technology
On the basis of work, explore using Protocols in Molecular Biology identification resistant variety a certain rice region plantation in whether resistant skill
Art is efficiently and effectively supported to be provided in production using the prevention and treatment that resistant variety carries out rice blast.
The specific technical proposal is:
Resistant rice varieties plant blast resisting identification method in specific rice region, include the following steps, rely on molecular biosciences
And Plant Pathology research means know that rice varieties promote and apply the nontoxic gene type of the Pyricularia oryzae population of rice region first
Structure, next knows the key resistance genotype of the rice varieties of popularization and application, and then two results are compared, with
And comprehensive inoculation is carried out to rice varieties using rice region flora, finally efficiently identify whether rice resistant variety answers in quasi- popularization
Rice region shows resistance.
Resistant rice varieties plant blast resisting identification method in specific rice region, and steps are as follows for specific method:
(1) avirulence gene of rice blast type analysis
(1.1) Pyricularia oryzae single-ascospore strain is obtained
Based on tissue isolation, moisturizing culture passes through in the quasi- panicle blast sample for promoting and applying rice region acquisition of rice varieties
Spore cultivation descends slowly and lightly, microscopy, label, positioning, picking, culture, obtains Pyricularia oryzae single-ascospore strain, saves backup；
(1.2) Pyricularia oryzae single-ascospore strain genomic DNA is obtained
The genomic DNA of Pyricularia oryzae single-ascospore strain is extracted using CTAB method；
(1.3) know the nontoxic gene structure of rice region rice blast flora
Utilize the avirulence gene of rice blast specific primer constructed, difference PCR amplification Pyricularia oryzae single-ascospore strain
DNA knows the nontoxic gene type of single-ascospore strain by strip analysis, carries out the nontoxic gene that flora is known in data analysis to this
Structure；
(2) Resistance of Rice Varieties genetic analysis
(2.1) resistant variety genomic DNA is obtained
The genomic DNA of Pyricularia oryzae single-ascospore strain is extracted using CTAB method；
(2.2) know Resistance of Rice Varieties genotype
Using the Resistance of Rice Varieties gene-specific primer constructed, PCR amplification rice varieties DNA, passes through item respectively
Band analysis, knows the resistant genotype that rice varieties have；
(3) comparison of resistant genotype and nontoxic gene type is analyzed
Compare the resistant genotype of rice varieties and the nontoxic gene type of rice blast flora, if resistant genotype with it is nontoxic
Genotype is corresponding to be existed, and indicates that resistant genotype agrees with nontoxic gene type, which has in rice region Production of Large Fields
Resistance；If resistant gene does not agree with the not corresponding presence of nontoxic gene, expression resistant genotype with main nontoxic gene type；
(4) Rice Blast resistance indoor inoculation identification and analysis
Spray inoculation rice blast flora is carried out with the not corresponding existing rice varieties of nontoxic gene in indoor antagonism gene
Mixing conidium liquid, resistance result is obtained after 7d；
If blast resistance identification result is resistance, although showing the resistant genotype detected and nontoxic gene type not
It is corresponding to exist, but show that rice varieties have unknown resistant gene, rice blast flora has unknown nontoxic gene type, and
The two is corresponding to be existed, and the Rice Varieties seasonal febrile diseases are resistant in Production of Large Fields；
If blast resistance identification result be it is susceptible, show resistant gene in the rice varieties and rice blast flora
Nontoxic gene type is there is no agreeing with, and the Rice Varieties seasonal febrile diseases do not have resistance in Production of Large Fields.
Compared with the prior art, technical advantage that the present invention has:
Whether the present invention judges to obtain the rice resistant variety of resistance qualifications in the production of specific rice region with " anti-
Property ", blindness is avoided, to be pointedly efficiently the innovative point of this technology using resistant variety: by resistant gene and nothing
The Molecular Detection of virus gene, it is such as corresponding to exist, then show that the kind is resistant in Production of Large Fields；Exist if not corresponding, then
Further by spraying qualification result, distinguish whether the kind is resistant in Production of Large Fields.It through the invention, can be greatly
It reduces because blindness is using the loss in the production of resistant variety bring, resistant variety is allowed veritably to play " resistance " effect.
Specific embodiment
It is described in conjunction with the embodiments technical solution of the present invention.
Rice dryland rice kind Prince of the Devils paddy bestows favour in Magnaporthe grisea in Hubei Province and identifies sick nursery point (having identification qualification point) for 2009
Identification has middle blast resisting；Feel in occurring in the ground field production such as Hubei Tianmen, flat peach or height feels rice blast.(consigner
It queries thus, it is believed that the qualification result provided is wrong.)
Recently the applicant carries out experimental analysis using method proposed by the present invention, and flora (is divided in a manner of artificial infection
Reason region is divided into Hubei Province, the rice regions such as Southeastern Hubei, E Bei, west place in Hubei, E Dongbei, Jianghan Plain) Vitro Inoculation Technique, inoculation is magic as the result is shown
The Resistant expression of Wang Gu is not consistent, and west place in Hubei shows resistance, and it is susceptible that resistance, Southeastern Hubei and Jianghan Plain performance are showed in Hubei Province.Into
One step completes following analysis:
Extract Prince of the Devils paddy complete genome DNA, PCR product through PAGE glue electrophoresis, silver staining detection after, do not occur pi-kp,
The band of 9 resistant genes such as pi-i, pi-ta2, pi-9, pi-sh, pi-z, pi-kh, pi-1, pi-z5, shows in Prince of the Devils paddy
Without this 9 resistant genes.
The single-ascospore strain DNA of each rice region flora is extracted, PCR product is detected through agarose gel electrophoresis, the pillar location of appearance
It is different, derive nontoxic gene type and the frequency of occurrences that flora is included.Nontoxic gene type type possessed by each rice region and go out
Existing frequency is different.
Engage the resistant gene testing result, the nontoxic gene type of each rice region flora composition and frequency of occurrences feelings of Prince of the Devils paddy
Although the artificial infection of condition and Prince of the Devils paddy through each rice region flora is as a result, can clearly know that Prince of the Devils paddy is having qualified rice
Seasonal febrile diseases identification garden (west place in Hubei rice region) identification resists in having, but its " resistance " is not same " presence " in the other rice regions in Hubei Province.
It include the list of 24 known types by other set since Prince of the Devils paddy does not have 9 genotype of single-gene identification strain
The artificial infection of spore bacterial strain, can obtain the resistant genotype for pushing away Prince of the Devils paddy, and composition situation and its of resistant gene show the rice of resistance
The nontoxic gene type of area's flora, which is constituted, coincide (consistent with high-frequency nontoxic gene type) substantially.
This embodiment absolutely proves that it is objective reality, no that the resistant gene of kind, which is the inhereditary material that kind has,
Change with the rice region of its plantation, but it is a kind of phenomenon that whether it, which shows resistance, be not it is unalterable, Resistant expression with
The flora nontoxic gene type composition of planted rice region is highly relevant, that is, compares the resistant genotype of analysis kind and the nothing of flora
Virus gene type can know (whether blast resisting has become the precondition that rice material promotes and applies to resistant variety, is both needed to qualification
The identification of Department certification garden, in resist above can promote and apply) " resistance " can really show.
Due to the understanding and history to rice blast, rice blast qualification Department certification garden is generally set to rice blast Chang Chong
Send out area, area can effectively identify resistance situation to rice blast to rice material herein, but the rice blast in these areas
Flora is not all representative, therefore blindly will lead to rice resistant variety in other rice regions using so-called resistant variety
Resistance is not showed, brings unnecessary loss to production.
This example demonstrates that can effectively identify rice resistant variety specific using the method for offer of the invention
Whether there is " resistance " in the production of rice region, avoid blindness, to efficiently pointedly utilize resistant variety.
Claims (2)
1. resistant rice varieties plant blast resisting identification method in specific rice region, it is characterised in that: include the following steps, rely on
Molecular biology and Plant Pathology research means know that rice varieties promote and apply the nothing of the Pyricularia oryzae population of rice region first
Virus gene type structure, next knows the key resistance genotype of the rice varieties of popularization and application, then compares two results
Comprehensive inoculation is carried out to rice varieties to analysis, and using rice region flora, finally efficiently whether identifies rice resistant variety
Resistance is showed in the quasi- rice region promoted and applied.
2. resistant rice varieties according to claim 1 plant blast resisting identification method in specific rice region, feature exists
In: specifically includes the following steps:
(1) avirulence gene of rice blast type analysis
(1.1) Pyricularia oryzae single-ascospore strain is obtained
Based on tissue isolation, moisturizing culture passes through spore in the quasi- panicle blast sample for promoting and applying rice region acquisition of rice varieties
It cultivates, descend slowly and lightly, microscopy, label, positioning, picking, culture, obtaining Pyricularia oryzae single-ascospore strain, save backup；
(1.2) Pyricularia oryzae single-ascospore strain genomic DNA is obtained
The genomic DNA of Pyricularia oryzae single-ascospore strain is extracted using CTAB method；
(1.3) know the nontoxic gene structure of rice region rice blast flora
Using the avirulence gene of rice blast specific primer constructed, PCR amplification Pyricularia oryzae single-ascospore strain DNA, leads to respectively
Strip analysis is crossed, knows the nontoxic gene type of single-ascospore strain, the nontoxic gene structure that flora is known in data analysis is carried out to this；
(2) Resistance of Rice Varieties genetic analysis
(2.1) resistant variety genomic DNA is obtained
The genomic DNA of Pyricularia oryzae single-ascospore strain is extracted using CTAB method；
(2.2) know Resistance of Rice Varieties genotype
Using the Resistance of Rice Varieties gene-specific primer constructed, PCR amplification rice varieties DNA, passes through band point respectively
Analysis, knows the resistant genotype that rice varieties have；
(3) comparison of resistant genotype and nontoxic gene type is analyzed
Compare the resistant genotype of rice varieties and the nontoxic gene type of rice blast flora, if resistant genotype and nontoxic gene
Type is corresponding to be existed, and indicates that resistant genotype agrees with nontoxic gene type, which has resistance in rice region Production of Large Fields；
If resistant gene does not agree with the not corresponding presence of nontoxic gene, expression resistant genotype with main nontoxic gene type；
(4) Rice Blast resistance indoor inoculation identification and analysis
The mixed of spray inoculation rice blast flora is carried out with the not corresponding existing rice varieties of nontoxic gene in indoor antagonism gene
Conidium liquid is closed, obtains resistance result after 7d；
If blast resistance identification result is resistance, although showing that the resistant genotype detected and nontoxic gene type be not corresponding
In the presence of, but showing that rice varieties have unknown resistant gene, rice blast flora has unknown nontoxic gene type, and the two
Corresponding to exist, the Rice Varieties seasonal febrile diseases are resistant in Production of Large Fields；
If blast resistance identification result be it is susceptible, show the nontoxic of resistant gene in the rice varieties and rice blast flora
Genotype is there is no agreeing with, and the Rice Varieties seasonal febrile diseases do not have resistance in Production of Large Fields.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910585583.1A CN110257473A (en) | 2019-07-01 | 2019-07-01 | Resistant rice varieties plant blast resisting identification method in specific rice region |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910585583.1A CN110257473A (en) | 2019-07-01 | 2019-07-01 | Resistant rice varieties plant blast resisting identification method in specific rice region |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110257473A true CN110257473A (en) | 2019-09-20 |
Family
ID=67923612
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910585583.1A Pending CN110257473A (en) | 2019-07-01 | 2019-07-01 | Resistant rice varieties plant blast resisting identification method in specific rice region |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110257473A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662953A (en) * | 2020-06-11 | 2020-09-15 | 华南农业大学 | Technical system capable of identifying disease-resistant genes by inoculating pathogens in different plant disease systems |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240346A (en) * | 2008-03-13 | 2008-08-13 | 云南农业大学 | Method for analyzing rice blast resisting gene |
| CN102534010A (en) * | 2012-01-17 | 2012-07-04 | 中国农业科学院作物科学研究所 | Primers for molecular detection of nontoxic genes of Magnaporthe grisea and application thereof |
| CN104404152A (en) * | 2014-12-03 | 2015-03-11 | 云南农业大学 | Genotype-expression molecular marker for detecting basic resistance of rice pyricularia grisea and application |
| CN112889664A (en) * | 2021-01-19 | 2021-06-04 | 江苏里下河地区农业科学研究所 | Method for cultivating broad-spectrum and durable resistant rice breeding material by polymerizing complementary rice blast resistant genes |
-
2019
- 2019-07-01 CN CN201910585583.1A patent/CN110257473A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240346A (en) * | 2008-03-13 | 2008-08-13 | 云南农业大学 | Method for analyzing rice blast resisting gene |
| CN102534010A (en) * | 2012-01-17 | 2012-07-04 | 中国农业科学院作物科学研究所 | Primers for molecular detection of nontoxic genes of Magnaporthe grisea and application thereof |
| CN104404152A (en) * | 2014-12-03 | 2015-03-11 | 云南农业大学 | Genotype-expression molecular marker for detecting basic resistance of rice pyricularia grisea and application |
| CN112889664A (en) * | 2021-01-19 | 2021-06-04 | 江苏里下河地区农业科学研究所 | Method for cultivating broad-spectrum and durable resistant rice breeding material by polymerizing complementary rice blast resistant genes |
Non-Patent Citations (1)
| Title |
|---|
| 朱昳: "湖北省稻瘟病菌无毒基因分析及水稻品种抗瘟基因型和田间抗性鉴定", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662953A (en) * | 2020-06-11 | 2020-09-15 | 华南农业大学 | Technical system capable of identifying disease-resistant genes by inoculating pathogens in different plant disease systems |
| CN111662953B (en) * | 2020-06-11 | 2021-05-04 | 华南农业大学 | Technical system capable of identifying disease-resistant genes by inoculating pathogens in different plant disease systems |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Navathe et al. | ToxA–Tsn1 interaction for spot blotch susceptibility in Indian wheat: An example of inverse gene-for-gene relationship | |
| Ofek-Lalzar et al. | Diversity of fungal endophytes in recent and ancient wheat ancestors Triticum dicoccoides and Aegilops sharonensis | |
| Offre et al. | Identification of bacterial groups preferentially associated with mycorrhizal roots of Medicago truncatula | |
| Shi et al. | Comparative analysis of the rhizomicrobiome of the wild versus cultivated crop: insights from rice and soybean | |
| Moslemi et al. | Paraphoma crown rot of pyrethrum (Tanacetum cinerariifolium) | |
| Khadka et al. | Study on differential response of Pyricularia grisea isolates from rice, finger millet and Panicum sp. with local and alien media, and their host range | |
| CN110257473A (en) | Resistant rice varieties plant blast resisting identification method in specific rice region | |
| CN105210749B (en) | A kind of method for improving rice blast neck blast resistance identification accuracy | |
| Ünal et al. | Evaluation of disease intensity and molecular identification of Zymoseptoria tritici causing Septoria leaf blotch on wheat in the Eastern Mediterranean Region of Turkey | |
| CN104232748B (en) | Whether a kind of red bayberry nursery stock carries the rapid molecular detection method of wilting germ | |
| Casini et al. | Endophytic fungal communities of ancient wheat varieties | |
| YONES et al. | Molecular characterization of Trichoderma spp. with biocontrol ability against faba bean chocolate spot (Botrytis cinerea Pers. ex Fr.) | |
| Shanqi et al. | Leaf disease caused by Epicoccum thailandicum on Amomum villosum in China | |
| KR20060104319A (en) | Specific primer for identification of cordyceps militaris and identification method using the same | |
| CN105462971A (en) | Specific molecular marker for rice blast resistance gene Pi2 and dedicated primers thereof | |
| Arabi et al. | Expression of resistance to Blumeria graminis in barley genotypes (Hordeum vulgare L.) under field and controlled conditions | |
| Rahnama et al. | First report of the blast pathogen, Pyricularia oryzae, on Eragrostis tef in the United States | |
| Ilyushko et al. | Resistance of Far Eastern Rice Oryza Sativa L. Varieties and Competitive Testing Samples to Pyricularia Oryzae Cav. | |
| Parinthawong et al. | Pathogenicity of Pyricularia oryzae on elite rice cultivars and geographical distribution of avirulence genes causing blast disease in Thailand. | |
| CN106222097A (en) | A kind of isolation and identification method of sorghum hybrid sudangrass Leaf blotch pathogeny | |
| Olivas-Peraza et al. | Curvularia muehlenbeckiae causing leaf spot on Johnson grass in Mexico | |
| Mukherjee et al. | Effect of nitrogen fertilization on the expression of slow-blasting resistance in rice | |
| Gaffoor et al. | Performance of novel sorghum germplasm in Pennsylvania and their response to anthracnose | |
| Pathmanathan et al. | Comparison of colonization, diversity, and molecular phylogeny of endophytic fungi in selected traditional and newly improved rice (Oryza sativa L.) varieties in Sri Lanka | |
| Nguyen et al. | Molecular characterisation of Proxipyricularia zingiberis causing blast disease of ginger in Vietnam |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190920 |
|
| RJ01 | Rejection of invention patent application after publication |