CN110257301B - Lactobacillus fermentation liquor with fishy smell removing and taste enhancing effects and application thereof - Google Patents

Lactobacillus fermentation liquor with fishy smell removing and taste enhancing effects and application thereof Download PDF

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CN110257301B
CN110257301B CN201910643079.2A CN201910643079A CN110257301B CN 110257301 B CN110257301 B CN 110257301B CN 201910643079 A CN201910643079 A CN 201910643079A CN 110257301 B CN110257301 B CN 110257301B
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fermentation liquor
fishy smell
use according
lactobacillus
lactobacillus fermentation
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刘文美
邹泽华
邓宏辉
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Yisheng Vitality Health Technology Fujian Co ltd
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Xiamen Hemei Kesheng Biological Technology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/70Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor
    • A23L13/72Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor using additives, e.g. by injection of solutions
    • A23L13/74Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor using additives, e.g. by injection of solutions using microorganisms or enzymes
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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

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Abstract

The invention discloses a lactobacillus fermentation liquor with fishy smell removing and taste enhancing effects and application thereof. The preparation method of the lactobacillus fermentation liquor comprises sterilizing the propagation culture medium at high temperature, cooling to 30-40 deg.C, inoculating Lactobacillus plantarum HM6068 seed solution, fermenting, inactivating at 95 deg.C for 30min, and bottling to obtain lactobacillus fermentation liquor; the expanding culture medium contains white granulated sugar, yeast extract, soybean extract, magnesium sulfate and purified water. It has good fishy smell removing effect at low temperature and short time.

Description

Lactobacillus fermentation liquor with fishy smell removing and taste enhancing effects and application thereof
Technical Field
The invention relates to the field of microorganisms, in particular to a lactobacillus fermentation liquid with fishy smell removing and taste enhancing effects and application thereof.
Background
The existing deodorization method mainly comprises three major methods, namely a physical method, a chemical method and a biological method. The biological deodorization method has the advantages that the biological deodorization method is incomparable with other methods, and is a research hotspot, and the main characteristic is that the fishy smell and the peculiar smell of aquatic products are effectively removed through a fermentation technology, and special fragrance can be generated. But when the fishy smell is removed biologically, the fermentation time of the microorganism is long, and the fermentation needs higher temperature action, which affects the meat product.
Disclosure of Invention
The invention aims to provide the lactobacillus fermentation liquor with better fishy smell removing effect at low temperature, and the action time is short.
In order to achieve the aim, the invention provides a lactobacillus fermentation liquor which is characterized in that the preparation method comprises the steps of sterilizing a propagation culture medium at high temperature, cooling to 30-40 ℃, inoculating lactobacillus plantarum HM6068, fermenting, inactivating at 95 ℃ for 30min, and filling in a tank to obtain the lactobacillus fermentation liquor; the expanding culture medium contains white granulated sugar, yeast extract, soybean extract, magnesium sulfate and purified water.
Further, the high-temperature sterilization is performed at the temperature of 120-; preferably, the high-temperature sterilization is performed at 121 ℃ for 30 min.
Further, cooling to 35-40 ℃; preferably, the temperature is reduced to 37 ℃.
Further, the inoculation amount of the lactobacillus plantarum HM6068 is 1-2% by volume; preferably, the inoculation amount of the lactobacillus plantarum HM6068 is 1% by volume.
Further, the fermentation is performed at 35-40 ℃ for 10-30 hours, and preferably, the fermentation is performed at 37 ℃ for 18-24 hours.
Further, the inactivation temperature is 90-100 ℃, and the time is 20-40 min; preferably, the inactivation temperature is 95 ℃ and the inactivation time is 30 min.
Further, the expanding culture medium comprises 20 parts by weight of white granulated sugar, 10 parts by weight of yeast extract, 0.1 part by weight of soybean extract, 2 parts by weight of magnesium sulfate and 1000 parts by weight of purified water.
The invention also provides the application of the lactobacillus fermentation liquor in the processing process of meat and meat products for removing fishy smell and enhancing flavor.
The conventional microorganism deodorization is the effect of live bacteria through fermentation.
1. The process time is shortened, and the quality of meat products is improved: the conventional microorganism removes fishy smell, and live bacteria are selected for fermentation at a high temperature for a long time to achieve the fishy smell removing effect. For example, application No. 201710299852.9 relates to a biological deodorization technique for swimming bladder, which comprises culturing yeast and staphylococcus at 40 deg.C for 7 hr to obtain deodorization solution with an amount of 2-5 times of swimming bladder weight, and deodorization time of 1-2 hr.
The method of the invention mixes the inactivated bacteria with the meat product in a low temperature (below 4 ℃) environment, so that the metabolite is fully combined with the meat product, and the effect of removing fishy smell is achieved. The process time is shortened.
2. The addition of the metabolic products of the lactic acid bacteria can specifically promote the health effect of digestion and absorption.
3. The mouthfeel of the meat product after repeated freeze thawing is increased: after the product prepared by the strain is added, the meat product is repeatedly quick-frozen and melted, and the taste difference is small.
The following processing modes are as follows:
thawing duck meat, peeling, and controlling the temperature at 0-2 ℃;
slicing, and controlling the thickness of the slices to be 0.3-0.8mm preferably;
rolling and kneading: adding the ingredients in table 1; rolling and kneading under vacuum of 0.07MPA at 4 deg.C or below. And (3) packaging the treated duck meat into a freezer with the temperature of-10 ℃ for quick freezing, taking out the duck meat, and boiling the duck meat for the same time to taste the taste. The results are shown in Table 1.
Table 1: comparison table of effects of duck meat treated differently
Figure BDA0002132566490000021
Figure BDA0002132566490000031
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative of the invention and is not to be construed as limiting the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1: preparation of lactic acid bacteria fermentation liquor
Strain Lactobacillus plantarum HM 6068); see 201611104476.5, lactic acid bacteria fermentation liquor suitable for food processing application, and its preparation method and application.
Strain name: lactobacillus plantarum HM 6068;
date of deposit: 2015, 8 months and 10 days;
the deposit unit: china, Beijing, Haihu district, Zhongguancun, No. 13, China general microbiological culture Collection center (CGTCC);
deposit number: CGMCC No. 11214.
Expanding culture medium: 20 parts of white granulated sugar, 10 parts of yeast extract, 0.1 part of soybean extract, 2 parts of magnesium sulfate and 1000 parts of purified water.
The preparation method comprises the following steps: sterilizing the propagation culture medium at 121 deg.C for 30min, cooling to 37 deg.C, inoculating Lactobacillus plantarum HM6068 in an amount of 1% by volume, fermenting at 37 deg.C for 18-24 hr, inactivating at 95 deg.C for 30min, and canning to obtain lactobacillus fermentation liquid.
Example 2: deodorization treatment of duck meat
1. Thawing and peeling duck meat, and controlling the temperature of the duck meat to be 0-2 ℃ in the process; (in case of fresh duck meat, thawing is not required)
2. Cutting duck meat into slices, controlling the thickness of the slices to be 0.5mm, and cutting the duck meat into 1000g slices;
3. rolling and kneading: 50g of the lactic acid bacteria fermentation broth obtained in example 1 was added, 910g of water and 5g of a phosphorus-free water-retaining agent were added. And (4) uniformly mixing. Rolling and kneading for 60 minutes under the environment of vacuum 0.07MPA and the temperature below 4 ℃; can be rolled and kneaded manually or mechanically. Rou seven turns in one minute.
4. Adding 40g of cassava starch, and rolling and kneading for 20 minutes below 4 ℃.
5. Subpackaging, freezing and selling.
A control was set, and the groups are shown in table 2.
TABLE 2 grouping table of duck meat treated differently
Figure BDA0002132566490000032
Figure BDA0002132566490000041
The methods adopted in the above groups are the same as above. The judgment criteria of the results are shown in Table 3. The results are shown in tables 4 to 5.
TABLE 3 judgment standards Table
Figure BDA0002132566490000042
Table 4 experimental results table 1
Figure BDA0002132566490000043
Figure BDA0002132566490000051
From the results of table 4, table 5 was obtained in summary.
Table 5 experimental results table 2
Figure BDA0002132566490000052
It can be seen that the lactobacillus fermentation liquor of the invention has the best effect on removing fishy smell of duck meat. The effect is far higher than that of the commercial lactobacillus plantarum No. 1-3, and is far higher than that of a blank culture medium, water and untreated medium. The adoption of the microbial deodorization method leads the taste of the meat product to be poor and has other foreign flavors because the fermentation treatment at higher temperature is required.
Example 3: fishy smell removing treatment for fish meat
1. Slicing fish, controlling the thickness of the slices to be 0.3mm, and keeping the fish slices to be 1000 g;
2. rolling and kneading: 50g of the lactic acid bacteria fermentation broth of example 1 was added and mixed well. Rolling and kneading for 10 minutes under the vacuum 0.07MPA environment, wherein the working temperature of rolling and kneading (one minute and five revolutions) is below 4 ℃;
3. subpackaging, freezing and selling.
The control example is a water-added control.
As a result, it was found that the fish meat with the lactic acid bacteria-containing fermentation broth had less fishy smell than the fish meat with water.
Example 4: deodorization treatment of pork
1. Slicing fish, controlling the thickness of the slices to be 0.5mm, and keeping the fish slices to be 1000 g;
2. rolling and kneading: adding 50g of lactobacillus fermentation liquor, and uniformly mixing. Adding 910g of water and 5g of a phosphorus-free water-retaining agent; mixing, kneading under vacuum 0.07MPA for 60 min at 4 deg.C or below;
3. adding 20g of cassava starch, and rolling and kneading for 10 minutes;
4. subpackaging, freezing and selling.
The control example is a water-added control. As a result, it was found that the fishy smell of pork obtained by adding the lactic acid bacteria-containing fermentation broth was less than that of pork obtained by adding water.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made in the above embodiments by those of ordinary skill in the art without departing from the principle and spirit of the present invention.

Claims (10)

1. The preparation method of the lactobacillus fermentation liquor comprises the steps of sterilizing a culture expanding medium at high temperature, cooling to 30-40 ℃, inoculating lactobacillus plantarum HM6068 with the preservation number of CGMCC number 11214, fermenting, inactivating at 95 ℃ for 30min, and canning to obtain the lactobacillus fermentation liquor; the expanding culture medium contains white granulated sugar, yeast extract, soybean extract, magnesium sulfate and purified water; the low temperature is below 4 ℃.
2. The method as claimed in claim 1, wherein the high temperature sterilization is 120-130 ℃ sterilization for 20-40 min.
3. The use according to claim 1, wherein the high temperature sterilization is sterilization at 121 ℃ for 30 min.
4. The use according to claim 1, wherein the cooling is to a temperature of 35-40 ℃.
5. The use according to claim 1, wherein the cooling is to 37 ℃.
6. Use according to claim 1, wherein the lactobacillus plantarum HM6068 is inoculated in an amount of 1-2% by volume.
7. Use according to claim 1, wherein the lactobacillus plantarum HM6068 inoculum size is 1% by volume.
8. Use according to claim 1, wherein the fermentation is at 35-40 ℃ for 10-30 hours.
9. Use according to claim 1, wherein the fermentation is at 37 ℃ for 18 to 24 hours.
10. The use of claim 1, wherein the propagation medium comprises 20 parts by weight of white granulated sugar, 10 parts by weight of yeast extract, 0.1 part by weight of soybean extract, 2 parts by weight of magnesium sulfate and 1000 parts by weight of purified water.
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