CN110257279A - Degrading organic phosphor pesticides L-1 bacterial strain and its application - Google Patents
Degrading organic phosphor pesticides L-1 bacterial strain and its application Download PDFInfo
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- CN110257279A CN110257279A CN201910470093.7A CN201910470093A CN110257279A CN 110257279 A CN110257279 A CN 110257279A CN 201910470093 A CN201910470093 A CN 201910470093A CN 110257279 A CN110257279 A CN 110257279A
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- Prior art keywords
- bacterial strain
- organic phosphor
- degrading organic
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- parathion
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
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- Business, Economics & Management (AREA)
- General Chemical & Material Sciences (AREA)
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- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of L-1 bacterial strain of degrading organic phosphor pesticides and its application, which being capable of effective degrading organic phosphor pesticides, especially parathion-methyl or metrifonate.
Description
Technical field
The present invention relates to a kind of bacterial strain more particularly to a degrading organophosphorus pesticide L-1 bacterial strains.
Background technique
With the increasingly raising of China's economic development and living standards of the people, the people increasingly focus on asking for food safety
Topic, in recent years, China's food-safety problem happens occasionally, and causes the extensive concern of people.And China is large agricultural country, agricultural production
Product occupy the 70% of China's food consumption total amount as main food consumption type.Pesticide must can not as agricultural modernization
Few means of production are reducing crop pests, increase agricultural output, and raising agricultural product quality etc. plays huge
Effect.But unreasonable during people prevent and treat diseases and pests of agronomic crop and overdose uses chemical pesticide, causes in agricultural product
Persticide residue it is higher and higher, cause serious Practice for Pesticide Residue in Agricultural Products or even direct contaminated food products, threaten human health,
Serious contaminated soil, water body and atmospheric environment, reduce the diversity of surrounding biologic population, destroy the balance of the ecosystem, together
When also affect the people health and agricultural product outlet, destroy agroecological environment.
Currently, many to the biodegrading process of pesticide both at home and abroad, in different biodegrading process, biodegrade is most important
One of method.Microorganism is because it is more with type, distribution is wide, breeding is fast, Yi Bianyi, has the spies such as very strong biochemical adaptability
Point, the often final degradation pathway of pollutant.Therefore, carrying out the degradation of pesticide using microorganism is many pollution control scholars
Research emphasis.But the bacterial strain for capableing of effectively degrading organic phosphor pesticides at present is also more rare.
Summary of the invention
The object of the present invention is to provide a degrading organophosphorus pesticide L-1 bacterial strain, which being capable of effective degrading organic phosphor
Pesticide.
Bacterial strain provided by the present invention picks up from the farmland that organophosphorus pesticide is often sprayed in Hebei province Baoding.With preparatory sterilizing
Small scoop take 100 g of soil sample under surface soil and earth's surface at 5 ~ 10 cm or so, be contained in the kraft paper bag to sterilize in advance, tighten.
Bacterial strain can be grown on the culture medium containing parathion-methyl, and screening is in the growth of high concentration agricultural chemical side and bacterium colony
The different bacterial strain of form carries out scribing line and isolates and purifies, and it is L-1 that preservation, which isolates single colonie and as screens obtained Strain Designation,.
The obtained colonial morphology of bacterial strain L-1 is screened as shown in Figure 1, bacterium colony is rounded, edge is irregular, dry tack free and
There is fold, colony colour is White-opalescent, and Liquid Culture forms mycoderm and layering is obvious.After Gram's staining, in optics
For microscopically observation as shown in Fig. 2, Gram-positive, thallus is rod-shaped, and is individually arranged, and has gemma.
The part Physiology and biochemistry identification of indicator of bacterial strain L-1 is as follows, can utilize sucrose, cellobiose, xylose, glycerol, sorb
Alcohol, mannitol, gelatin liquefaction can utilize citrate, catalase positive.Specific Physiology and biochemistry qualification result is shown in Table 1.
The 16S rDNA gene order of bacterial strain L-1 is analyzed, fragment length is 1454 bp.It is compared through gene order
Analysis, phylogenetic evolution tree constructed by the 16S rDNA gene order with them as shown in figure 3, bacterial strain L-1 andBacillus sp.Lzh-5(CP024647)、Bacillus velezensis S141(AP018402)、Bacillus velezensis strain NKG-1(CP024203) sequence is in same branch, and homology is up to 100%.Therefore bacterium is combined
Colonial morphology, displaing microstructure observing and the physio-biochemical characteristics of strain, are finally accredited as bacillus for bacterial strain L-1
(Bacillus)。
L-1 bacillus of the invention, can effective degrading organic phosphor pesticides, to cypermethrin (pyrethroid agriculture
Medicine) degradation rate: 68.76%；To carbofuran (carbamate chemicals for agriculture) 50.2%；To atrazine (heterocyclic pesticide) 61.51%,
Bacterial strain has good degradation effect to parathion-methyl and metrifonate, and degradation rate respectively reaches 74.43% and 70.45%, degradation
Effect is preferable.
Detailed description of the invention
Fig. 1 is the colonial morphology of bacterial strain L-1；
Fig. 2 is bacterial strain through Gram's staining figure；
Fig. 3 is the phylogenetic tree of the 16S r DNA building based on bacterial strain L-1；
The standard curve of Fig. 4 parathion-methyl；
Fig. 5 parathion-methyl goes out peak figure；
The standard curve of Fig. 6 metrifonate；
Fig. 7 metrifonate goes out peak figure.
Specific embodiment
The present invention is further illustrated below by specific embodiment.
The chromatogram and standard curve of 1 parathion-methyl of embodiment and metrifonate
It is detected under optimized feasible chromatographic condition, it is qualitative with retention time, measure parathion-methyl and metrifonate
Standard curve, the standard curve of parathion-methyl and peak figure is as shown in Figure 4,5 out, calibration curve equation are as follows: y=1E+06x+
30543, R2=0.9989, linear relationship is good；The standard curve of metrifonate and peak figure is as shown in Figure 6,7 out, calibration curve equation
Are as follows: y=6E+06x- 282371, R2=0.9981, linear relationship are good.
Degradation effect of the bacterial strain to parathion-methyl and metrifonate
As shown in table 2, the sample gas Chromatographic Determination that will be handled well obtains bacterial strain L-1 to parathion-methyl and metrifonate
The rate of recovery and degradation rate, the degradation rate to parathion-methyl are 73.43%, and the degradation rate to metrifonate is 70.45%, bacterial strain L-1
It is preferable to the degradation effect of both pesticides.
It is obtained using similar experimental method, the bacterial strain is to cypermethrin (pyrethroid pesticide) degradation rate 68.76%；
To carbofuran (carbamate chemicals for agriculture) degradation rate 50.2%；To atrazine (heterocyclic pesticide) degradation rate 61.51%.
Claims (2)
1. a kind of L-1 bacterial strain of degrading organic phosphor pesticides.
2. application of the bacterial strain as described in claim 1 in degrading organic phosphor pesticides, which is characterized in that the organophosphorus pesticide
For parathion-methyl or metrifonate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910470093.7A CN110257279A (en) | 2019-05-31 | 2019-05-31 | Degrading organic phosphor pesticides L-1 bacterial strain and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910470093.7A CN110257279A (en) | 2019-05-31 | 2019-05-31 | Degrading organic phosphor pesticides L-1 bacterial strain and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110257279A true CN110257279A (en) | 2019-09-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910470093.7A Pending CN110257279A (en) | 2019-05-31 | 2019-05-31 | Degrading organic phosphor pesticides L-1 bacterial strain and its application |
Country Status (1)
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101024819A (en) * | 2006-02-20 | 2007-08-29 | 中国科学院动物研究所 | DU-E4LP seudomonas putida and use in degradating parathionmethyl |
| RU2011128996A (en) * | 2011-07-13 | 2013-01-20 | Общество с ограниченной ответственностью "БИОЛЭНД" | PHENOX BIOLOGICAL PRODUCT FOR PURIFICATION OF WATER, INDUSTRIAL WASTE AND SOIL FROM SUSTAINABLE TO DECOMPOSITION OF CHEMICALS AND METHOD OF ITS APPLICATION |
| CN104988101A (en) * | 2015-08-03 | 2015-10-21 | 黑龙江省科学院微生物研究所 | Bacillus cereus and application of bacillus cereus as agricultural biological agent |
| CN109321631A (en) * | 2018-10-08 | 2019-02-12 | 山东蔚蓝绿源生物科技有限公司 | A kind of Study on Fermentation of the bacillus laterosporus of degradable organophosphorus pesticide |
-
2019
- 2019-05-31 CN CN201910470093.7A patent/CN110257279A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101024819A (en) * | 2006-02-20 | 2007-08-29 | 中国科学院动物研究所 | DU-E4LP seudomonas putida and use in degradating parathionmethyl |
| RU2011128996A (en) * | 2011-07-13 | 2013-01-20 | Общество с ограниченной ответственностью "БИОЛЭНД" | PHENOX BIOLOGICAL PRODUCT FOR PURIFICATION OF WATER, INDUSTRIAL WASTE AND SOIL FROM SUSTAINABLE TO DECOMPOSITION OF CHEMICALS AND METHOD OF ITS APPLICATION |
| CN104988101A (en) * | 2015-08-03 | 2015-10-21 | 黑龙江省科学院微生物研究所 | Bacillus cereus and application of bacillus cereus as agricultural biological agent |
| CN109321631A (en) * | 2018-10-08 | 2019-02-12 | 山东蔚蓝绿源生物科技有限公司 | A kind of Study on Fermentation of the bacillus laterosporus of degradable organophosphorus pesticide |
Non-Patent Citations (3)
| Title |
|---|
| DI MENG等: "An alkaline phosphatase from Bacillus amyloliquefaciens YP6 of new application in biodegradation of five broad-spectrum organophosphorus pesticides", 《J ENVIRON SCI HEALTH B》 * |
| 段海明: "蜡状芽孢杆菌不同菌株降解有机磷农药的特性分析", 《生态环境学报》 * |
| 高熳熳等: "降解有机磷农药L-1菌株的筛选与鉴定", 《农药》 * |
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