CN110257235A - A kind of unicellular sequence testing chip automatic clamping device - Google Patents
A kind of unicellular sequence testing chip automatic clamping device Download PDFInfo
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- CN110257235A CN110257235A CN201910467747.0A CN201910467747A CN110257235A CN 110257235 A CN110257235 A CN 110257235A CN 201910467747 A CN201910467747 A CN 201910467747A CN 110257235 A CN110257235 A CN 110257235A
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- chip
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P70/00—Climate change mitigation technologies in the production process for final industrial or consumer products
- Y02P70/50—Manufacturing or production processes characterised by the final manufactured product
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Abstract
A kind of unicellular sequence testing chip automatic clamping device.The present apparatus is designed for a kind of unicellular sequence testing chip of Shanghai Lie Bing biological medicine Science and Technology Ltd..Installation positioning is realized by support plate in all parts of whole device, chip and chip clip slot pass through two conveyer belts laterally and longitudinally respectively and are sent into installation site, then mounting groove is promoted by slider-crank mechanism and then completes to install, experimenter only need to be in the chip that equipment side diversion trench takes clamping complete using chip when, the operating condition of equipment is noticed in the free time for carrying out unicellular sequencing, whether the quantity of chip and clip slot is sufficient, while the present invention demand reduced to labour largely, clamping efficiency is improved.
Description
Technical field
The invention belongs to the RNA sequence detection technique field of biological cell, in particular to a kind of unicellular sequence testing chip is certainly
Dynamic clamping device.
Background technique
Unicellular sequencing technologies refer in individual cells level, carry out high pass measurement to genome, transcript profile, apparent group
Sequence analysis a new technology, it can make up for it the limitation of traditional high-flux sequence, disclose individual cells gene structure and
Gene expression status reflects intercellular heterogeneity, and can analyze intercellular relationships in microenvironment.It is 2013, single
Cell sequencing technologies are classified as the six big field umber ones most to merit attention in year by " Science "；It climbs up again within 2015
Science translational medicine cover.Currently, unicellular sequencing technologies tumour, Developmental Biology, microbiology, Neuscience, with
And the fields such as botany play a significant role.The focus for just becoming life science, has broad application prospects.
Currently, unicellular sequencing needs to be aligned using sequence testing chip by the way of manually installed using card slot, then spiral shell
Silk is tightened.This method is inconvenient and time-consuming and laborious, affects to the period of entire unicellular sequencing experiment.
Summary of the invention
The object of the present invention is to provide a kind of unicellular sequence testing chip automatic clamping devices, carry out compared to the prior art single
Chip used in cell detection still by the way of manually fixing, solves time-consuming and laborious and inefficiency ask when fixed
Topic.
The technical scheme adopted by the invention is that a kind of unicellular sequence testing chip automatic clamping device, including support plate, branch
It is additionally provided with fixed turntable on fagging, is disposed with chip clip slot transmission device, chip transmission along fixed turntable direction of rotation
Device, receiving slit and discharge mechanism；
Fixed turntable, fixed turntable are fixed on support plate surface and, the side of fixed turntable parallel with support plate by bearing
While being additionally provided with several limiting slots；
Chip clip slot transmission device, including crankshaft connecting rod propulsive mechanism, the crankshaft connecting rod propulsive mechanism include zero
Part bracket, sliding rail is fixed on part carriers, and sliding rail one end is provided with close to fixed turntable, sliding rail far from one end of fixed turntable
Crankshaft driving motor, crankshaft driving motor are connect by crankshaft-link rod with the sliding block of sliding rail is cooperated, and sliding rail is for delivering chip gripper
Slot, the side of sliding rail are also disposed with backing plate and block, and backing plate is used to prevent chip clip slot from falling with block, crankshaft connecting rod
Propulsive mechanism is used to deliver the limiting slot on chip clip slot to fixed turntable；
Chip transfer apparatus, chip transfer apparatus is identical as chip clip slot transmission device structure, and chip transfer apparatus is used for
Chip is delivered, chip clip slot is for clamping chip；
Receiving slit, receiving slit is for receiving the chip clip slot for being clamped with chip；
Discharge mechanism, including unloading push rod, unloading push rod be set to by rotary shaft it is in support plate and parallel with support plate,
One end of unloading push rod is located at the top at receiving slit and fixed turntable cooperation, and unloading push rod can be pivoted, on chip clip slot
It is additionally provided with the boss baffle with unloading push rod cooperation.
The features of the present invention also characterized in that:
Chip clip slot transmission device further includes conveyer belt, and conveyer belt is fixed in support plate by two transmission rollers, and two
A delivery roller is connected separately with motor, and conveyer belt one end is close to fixed turntable, and chip gripper slot device is for transporting chip clip slot
To the sliding rail of part carriers；
Wherein unloading push rod is also connected with drive rod by movable axis far from receiving slit one end, and drive rod is far from unloading push rod
One end disk is connected with by movable axis, the movable axis that drive rod is connect with disk is located at position of the disk far from the center of circle, circle
Disk setting is on the supporting plate and positioned at the side of fixed turntable, and the center of circle of disk and the center of circle of fixed turntable are in same level
On line；
Wherein the bottom of disk is also connected with the drive link coaxial with circle disk center, and drive link stretches out disk, and drives
One end that lever stretches out disk can protrude into the bottom of fixed turntable, and one end of drive rod stretching disk is additionally provided with boss, drives
Stepper motor is also connected in lever and the connecting shaft of excessive turntable；
The bottom for wherein fixing turntable is provided with several sliding slots cooperated with boss, and one end of sliding slot is located at fixed turntable side
Side and be opening, the closing of the sliding slot other end, the rotation position that the closed end of sliding slot is used to that boss to be cooperated to limit fixed turntable；
Wherein chip clip slot includes lower bottom base, and lower bottom base is groove-like, and the side of groove is opening, and groove is for placing
Chip further includes upper bed-plate, and upper bed-plate is covered on the groove of lower bottom base, and upper bed-plate passes through bolt and lower bottom base opening two sides
Connection；
Wherein spring is also arranged on bolt；
Wherein boss baffle is located at the top of upper bed-plate；
Wherein chip transfer apparatus further includes electric screw driver, and electric screw driver is located at the side of chip transfer apparatus, electricity
Dynamic screwdriver is used for fitted bolt；
Wherein electric screw driver is fixed in support plate by support rod, and support rod is vertically arranged with support plate, support rod
On be additionally provided with the dentation sliding rail parallel with support rod two sides, the side of electric screw driver is provided with the gear with sliding rail cooperation,
Stepper motor is also connected on gear.
The beneficial effects of the present invention are:
A kind of unicellular sequence testing chip automatic clamping device of the invention is determine automatically to sequence testing chip using machine
Position clamping while the demand reduced to labour largely, improves clamping efficiency.
Detailed description of the invention
Fig. 1 is a kind of unicellular sequence testing chip automatic clamping device structural schematic diagram of the invention；
Fig. 2 is that a kind of crankshaft connecting rod pushing mechanism structure of unicellular sequence testing chip automatic clamping device of the invention is shown
It is intended to；
Fig. 3 is a kind of unloading pusher structure schematic diagram of unicellular sequence testing chip automatic clamping device of the invention；
Fig. 4 is a kind of chip gripper slot structure schematic diagram of unicellular sequence testing chip automatic clamping device of the invention；
Fig. 5 is a kind of sliding groove structure schematic diagram of unicellular sequence testing chip automatic clamping device of the invention.
In figure, 1. unloading push rods, 2. fixed turntables, 3. support rods, 4. electric screw drivers, 5. conveyer belts, 6. boss baffles,
7. boss, 8. backing plates, 9. upper bed-plates, 10. crankshaft driving motors, 11. part carriers, 12. lower bottom bases, 13. bolts, 14. crankshafts
Connecting rod, 15. springs, 16. support plates, 17. drive links, 18. drive rods, 19. limiting slots, 20. disks, 21. sliding slots, 22. is sliding
Block, 23. receiving slits.
Specific embodiment
The following describes the present invention in detail with reference to the accompanying drawings and specific embodiments.
The present invention provides a kind of unicellular sequence testing chip automatic clamping device, including support plate 16, in the support plate 16
Be additionally provided with fixed turntable 2, along fixed 2 direction of rotation of turntable be disposed with chip clip slot transmission device, chip transfer apparatus,
Receiving slit and discharge mechanism；
Fixed turntable 2, the fixed turntable 2 are fixed on 16 surface of support plate and parallel with support plate 16 by bearing, Gu
The side for determining turntable 2 is additionally provided with several limiting slots 19；
Chip clip slot transmission device, the chip clip slot transmission device include conveyer belt 5, and conveyer belt 5 passes through two transmission
Roller is fixed in support plate 16, and two delivery rollers are connected separately with motor, and 5 one end of conveyer belt is close to fix turntable 2,
The chip gripper slot device cooperates for transporting chip clip slot, chip clip slot and limiting slot 19, and the chip transfer apparatus also wraps
Crankshaft connecting rod propulsive mechanism is included, the crankshaft connecting rod propulsive mechanism is located at conveyer belt 5 close to one end of fixed turntable 2；
Crankshaft connecting rod propulsive mechanism, including part carriers 11 are fixed with sliding rail, the sliding rail on the part carriers 11
It is vertically arranged with conveyer belt 5, the sliding rail is used to accept the chip that communicated band 5 transmits, and side of the sliding rail far from conveyer belt 5 is also
It is disposed with backing plate 8 and block, the backing plate (8) and block are additionally provided with and sliding slot for preventing chip from falling on sliding slot
The sliding block 22 of cooperation, sliding rail are provided with crankshaft driving motor 10 far from one end of fixed turntable 2, and crankshaft driving motor 10 passes through song
Axis connecting rod 14 is connect with sliding block；
Chip clip slot includes lower bottom base 12, and the lower bottom base 12 is groove-like, and the side of groove is opening, the groove
It further include upper bed-plate 9 for chip placement, upper bed-plate 9 is covered on the groove of lower bottom base 12, and the upper bed-plate 9 passes through bolt
13 connect with the opening of lower bottom base 12 two sides, spring 15 are also arranged on bolt 13, spring 15 can make upper bed-plate 9 and lower bottom base
There are enough space chip placements between 12, it further includes electric screw driver 4 that the boss baffle 6, which is located at the top of upper bed-plate 9,
The electric screw driver 4 is located at the side of chip transfer apparatus, and electric screw driver 4 is used for fitted bolt 13, the electric screw
Knife 4 is fixed in support plate 16 by support rod 3, and support rod 3 is vertically arranged with support plate 16, be additionally provided on support rod 3 and
The parallel dentation sliding rail in 3 two sides of support rod, the side of electric screw driver 4 are provided with the gear with sliding rail cooperation, also connect on gear
It is connected to stepper motor, electric screw driver 4 can be displaced by gear according to the actual situation；
Chip transfer apparatus, the chip transfer apparatus is identical as chip clip slot transmission device structure, chip transfer apparatus
For delivering chip, the chip clip slot is for clamping chip；
Receiving slit, the receiving slit is for receiving the chip clip slot for being clamped with chip；
Discharge mechanism, including unloading push rod 1, the unloading push rod 1 be set in support plate 16 by rotary shaft and with branch
Fagging 16 is parallel, and one end of unloading push rod 1 is located at the top at receiving slit and fixed 2 cooperation of turntable, and unloading push rod 1 can be revolved around axis
Turn, the boss baffle 6 on the chip clip slot is against unloading push rod 1；
The unloading push rod 1 is also connected with drive rod 18 by movable axis far from receiving slit one end, and drive rod 18 is separate to be unloaded
The one end for carrying push rod 1 is connected with disk 20 by movable axis, and the movable axis that the drive rod 18 is connect with disk 20 is located at disk
20 positions far from the center of circle, the disk 20 are arranged in support plate 16 and are located at the side of fixed turntable 2, and the circle of disk 20
The center of circle of the heart and fixed turntable 2 is in same horizontal line, and the bottom of the disk 20 is also connected with coaxial with 20 center of circle of disk
Drive link 17, drive link 17 stretch out disk 20, and drive rod 17 stretch out disk 20 one end can protrude into fixed turntable 2
Bottom, and one end of the stretching disk 20 of drive rod 17 is additionally provided with boss 7, goes back in drive rod 17 and the connecting shaft of excessive turntable 20
It is connected with stepper motor, the bottom of the fixed turntable 2 is provided with several sliding slots 21 cooperated with boss 7, one end of sliding slot 21
It positioned at fixed 2 side of turntable and is opening, sliding slot 21+
Other end closing, the rotation position that the closed end of the sliding slot 21 is used to that boss 7 to be cooperated to limit fixed turntable 2.
In use, in order to guarantee that electric screw driver 4 can be avoided with 13 proper alignment of Fig. 4 bolt because of stepper motor
Directly driving fixed turntable 2 leads to (fixed 2 angle of rotation of turntable error occur) there are error for angle, electric screw driver 4 occurs not
The problem of capable of being accurately positioned with bolt 13, drives drive link 17 by motor, rotates fixed disc 2, and drive rod 17 rotates
One week, fixed turntable 2 rotated 90 °, while smoothly unloading after the completion of sequence testing chip clamping for convenience, avoided the occurrence of unloading
Mechanism and fixed 2 rotational frequency of turntable are inconsistent, and phenomena such as collision, unloading driving is also rotated using the fixed turntable 2 of driving
Stepper motor, guarantee completion when fixed turntable 2 is stationary by the differential seat angle of disk 20 and drive link 17
Unloading to chip aid body drives drive rod 18 to move while disk 20 rotates, so that unloading push rod 1 be driven to carry out
It waves, so that unloading push rod 1 is pushed to push the convex block baffle 6 on chip clip slot, completes unloaded operation.
Drive link 17 rotates counterclockwise, then the boss 7 of drive link 17 and fixed 2 pan bottom sliding slot 21 of turntable cooperation
Fixed turntable 2 is set to rotate clockwise 90 °, in the embodiment of the present application, there are four neighboring vertical limiting slots for setting on fixed turntable 2
19, therefore as shown in figure 5, four sliding slots 21 are arranged in fixed 2 bottom of turntable, and be 90 degree, 21 turns of sliding slot between adjacent two sliding slot 21
Dynamic 90 degree, drive link 17 rotates one week, and the boss 7 of drive link 17 is slided into just in sliding slot 21, due to drive link 17 and
The direction of rotation of fixed turntable 2 is on the contrary, this ensures that boss 7 blocks just when fixed turntable 2 turns to required working position
In the closed end of sliding slot 21, it can guarantee that the angle that disk rotates every time is 90 ° whole in this way, avoid stepper motor from directly driving solid
Determine 2 angular errors of fixation turntable occurred when turntable 2 rotates by external disturbance or stepper motor rotation error, these angular errors
It will lead to 19 appearance position error of limiting slot, chip and the chip when carrying out chip clamping on chip clip slot and fixed turntable 2
Clip slot appearance position error causes entire chip clamping process to occur colliding or the phenomenon that extrusion equipment, damages the sequence testing chip
With complete equipment, unnecessary fund and time is caused to waste.
Claims (10)
1. a kind of unicellular sequence testing chip automatic clamping device, which is characterized in that including support plate (16), the support plate (16)
On be additionally provided with fixed turntable (2), along fixed turntable (2) direction of rotation is disposed with chip clip slot transmission device, chip passes
Send device, receiving slit and discharge mechanism；
Fixed turntable (2), the fixed turntable (2) are fixed on support plate (16) surface by bearing and put down with support plate (16)
The side of row, fixed turntable (2) is additionally provided with several limiting slots (19)；
Chip clip slot transmission device, including crankshaft connecting rod propulsive mechanism, the crankshaft connecting rod propulsive mechanism include part branch
Frame (11) is fixed with sliding rail on the part carriers (11), and described sliding rail one end is close to fixed turntable (2), and sliding rail is far from fixed
One end of turntable (2) is provided with crankshaft driving motor (10), and crankshaft driving motor (10) is slided by crankshaft-link rod (14) and cooperation
The sliding block of rail connects, and for the sliding rail for delivering chip clip slot, the side of sliding rail is also disposed with backing plate (8) and block, institute
Backing plate (8) is stated with block for preventing chip clip slot from falling, the crankshaft connecting rod propulsive mechanism is for delivering chip clip slot extremely
Limiting slot (19) on fixed turntable (2)；
Chip transfer apparatus, the chip transfer apparatus is identical as chip clip slot transmission device structure, and chip transfer apparatus is used for
Chip is delivered, the chip clip slot is for clamping chip；
Receiving slit, the receiving slit is for receiving the chip clip slot for being clamped with chip；
Discharge mechanism, including unloading push rod (1), the unloading push rod (1) be set on support plate (16) by rotary shaft and with
In parallel, one end of unloading push rod (1) is located at the top at receiving slit and fixed turntable (2) cooperation to support plate (16), unloads push rod
(1) it can be pivoted, the boss baffle (6) with unloading push rod (1) cooperation, boss baffle are additionally provided on the chip clip slot
(6) against unloading push rod (1).
2. a kind of unicellular sequence testing chip automatic clamping device according to claim 1, which is characterized in that the chip gripper
Slot transmission device further includes conveyer belt (5), and conveyer belt (5) is fixed on support plate (16) by two transmission rollers, two institutes
It states delivery roller and is connected separately with motor, sliding rail of conveyer belt (5) output end on part carriers (11), the chip clip slot
Device is used to transport the sliding rail of chip clip slot to part carriers (11).
3. a kind of unicellular sequence testing chip automatic clamping device according to claim 1, which is characterized in that the unloading pushes away
Bar (1) is also connected with drive rod (18) far from receiving slit one end by movable axis, and the one of separate unloading push rod (1) of drive rod (18)
End is connected with disk (20) by movable axis, and it is remote that the movable axis that the drive rod (18) connect with disk (20) is located at disk (20)
Position from the center of circle, the disk (20) are arranged on support plate (16) and are located at the side of fixed turntable (2), and disk (20)
The center of circle and the center of circle of fixed turntable (2) be in same horizontal line.
4. a kind of unicellular sequence testing chip automatic clamping device according to claim 3, which is characterized in that the disk
(20) bottom is also connected with the drive link (17) coaxial with disk (20) center of circle, and drive link (17) stretches out disk (20),
And one end of drive rod (17) stretching disk (20) can protrude into the bottom of fixed turntable (2), and drive rod (17) stretches out disk
(20) one end is additionally provided with boss (7), is also connected with stepper motor in the connecting shaft of drive rod (17) and disk (20).
5. a kind of unicellular sequence testing chip automatic clamping device according to claim 1 or 4, which is characterized in that described solid
The bottom for determining turntable (2) is provided with several sliding slots (21) cooperated with boss (7), and one end of sliding slot (21) is located at fixed turntable
(2) side and be opening, the closing of sliding slot (21) other end, the closed end of the sliding slot (21) are solid for cooperating boss (7) limitation
Determine the rotation position of turntable (2).
6. a kind of unicellular sequence testing chip automatic clamping device according to claim 1, which is characterized in that the chip gripper
Slot includes lower bottom base (12), and the lower bottom base (12) is groove-like, and the side of groove is opening, and the groove is for placing core
Piece further includes upper bed-plate (9), and upper bed-plate (9) is covered on the groove of lower bottom base (12), and the upper bed-plate (9) passes through bolt
(13) it is connect with lower bottom base (12) opening two sides.
7. a kind of unicellular sequence testing chip automatic clamping device according to claim 6, which is characterized in that the bolt
(13) spring (15) are also arranged on.
8. a kind of unicellular sequence testing chip automatic clamping device according to claim 1 or 6, which is characterized in that described convex
Platform baffle (6) is located at the top of upper bed-plate (9).
9. a kind of unicellular sequence testing chip automatic clamping device according to claim 6, which is characterized in that further include electronic
Screwdriver (4), the electric screw driver (4) are located at the side of chip transfer apparatus, and electric screw driver (4) is used for fitted bolt
(13)。
10. a kind of unicellular sequence testing chip automatic clamping device according to claim 9, which is characterized in that described electronic
Screwdriver (4) is fixed on support plate (16) by support rod (3), and support rod (3) is vertically arranged with support plate (16), support rod
(3) the dentation sliding rail parallel with support rod (3) two sides is additionally provided on, the side of electric screw driver (4) is provided with matches with sliding rail
The gear of conjunction is also connected with stepper motor on gear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910467747.0A CN110257235B (en) | 2019-05-31 | 2019-05-31 | Single cell sequencing chip automatic clamping device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910467747.0A CN110257235B (en) | 2019-05-31 | 2019-05-31 | Single cell sequencing chip automatic clamping device |
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| CN110257235A true CN110257235A (en) | 2019-09-20 |
| CN110257235B CN110257235B (en) | 2022-10-04 |
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| CN201910467747.0A Active CN110257235B (en) | 2019-05-31 | 2019-05-31 | Single cell sequencing chip automatic clamping device |
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| CN112708545A (en) * | 2020-12-01 | 2021-04-27 | 合肥国研汉因检测科技有限公司 | Liquid drop digital PCR detection device based on micro-fluidic chip |
| CN112850076A (en) * | 2020-12-29 | 2021-05-28 | 泉州芸台科技有限公司 | Automatic feeding equipment of die cutting machine |
| CN112916429A (en) * | 2021-03-22 | 2021-06-08 | 重庆电子工程职业学院 | Book sorting device based on artificial intelligence |
| CN115945569A (en) * | 2023-03-13 | 2023-04-11 | 烟台怡和汽车科技有限公司 | Auto parts punching machine |
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