CN110257053A - A kind of customized frame nucleic acid nano illuminator of shape and its preparation method and application - Google Patents
A kind of customized frame nucleic acid nano illuminator of shape and its preparation method and application Download PDFInfo
- Publication number
- CN110257053A CN110257053A CN201910589495.9A CN201910589495A CN110257053A CN 110257053 A CN110257053 A CN 110257053A CN 201910589495 A CN201910589495 A CN 201910589495A CN 110257053 A CN110257053 A CN 110257053A
- Authority
- CN
- China
- Prior art keywords
- hydrophobic
- nucleic acid
- dna
- shape
- frame nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
- A61K49/0054—Macromolecular compounds, i.e. oligomers, polymers, dendrimers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0063—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres
- A61K49/0069—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the agent being in a particular physical galenical form
- A61K49/0089—Particulate, powder, adsorbate, bead, sphere
- A61K49/0091—Microparticle, microcapsule, microbubble, microsphere, microbead, i.e. having a size or diameter higher or equal to 1 micrometer
- A61K49/0093—Nanoparticle, nanocapsule, nanobubble, nanosphere, nanobead, i.e. having a size or diameter smaller than 1 micrometer, e.g. polymeric nanoparticle
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/02—Use of particular materials as binders, particle coatings or suspension media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nanotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Epidemiology (AREA)
- Materials Engineering (AREA)
- Biotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- General Engineering & Computer Science (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacturing & Machinery (AREA)
- Medical Informatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Composite Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of customized frame nucleic acid nano illuminator of shape and its preparation method and application, the following steps are included: S1: by hydrophobic group modification to DNA it is single-stranded on, the single-stranded carry out self assembly of DNA so that hydrophobic group modification is designed by DNA nanostructure, obtains the customized frame nucleic acid nano hydrophobic cavity of shape；And S2: the frame nucleic acid nano hydrophobic cavity is mixed with the hydrophobic fluorescence molecule or hydrophobic drug for being dissolved in specific solvent according to certain way, so that hydrophobic fluorescence molecule or hydrophobic drug enter the frame nucleic acid nano hydrophobic cavity, a kind of customized frame nucleic acid nano illuminator of shape is prepared.The present invention not only solves the problem of fluorescence imaging field lacks the scale topography novel nano fluorescent illuminant that precisely controllable, fluorescence intensity can precisely quantify, excellent carrier also is provided for hydrophobic drug conveying, provides new Research Thinking for Imaging-PAM development.
Description
Technical field
The invention belongs to Bio-Nano-Materials fields, shine more particularly to a kind of customized frame nucleic acid nano of shape
Body and its preparation method and application.
Background technique
Photobiology is imaged as biology and medical research provides a large amount of biological informations, such as the spy of cell specific region
Sign, form and the expression and distribution of specific biological molecules etc., therefore Photobiology is imaged on the research of vital movement, disease
There is important value in terms of the exploration of mechanism.Wherein, the original position of target molecule, real-time monitoring may be implemented in Imaging-PAM,
Compared with other imaging methods, there are the advantages such as not damaged, high specific and high sensitivity.Therefore, Imaging-PAM is in disease
Disease diagnosis, the fields such as drug metabolism tracer and announcement biomolecule microcosmic effect have important application.At present fluorescence imaging and
When be widely used to the imagings of the different levels such as molecule, cell and tissue, realize special ion, large biological molecule and body
Interior special organ or the imaging of tumour etc..
With deepening continuously to intracellular dynamic life process research, Imaging-PAM turns in research Intracellular signals
It leads, the fine life process such as protein-protein interaction plays increasingly important role.In vivo applications, nano fluorescent
The size and shape of particle will affect its quantity and approach that enter cell, to influence it in living body real time imagery to target
The targeting binding ability of molecule, and traditional synthetic method is also difficult to the accurate dimension and shape of control nano fluorescent particle now
Looks.Meanwhile being limited by traditional fluorescence probe, current Imaging-PAM master is to solve " with/without " fluorescence signal
Two-dimensional problem can not provide the order of information such as " strong/weak " that fluorescence signal precisely quantifies.However, organism is one extremely multiple
Whether miscellaneous dynamic equilibrium system, in many cases, the dosage of noxious material have been more than " threshold value " ratio that organism can bear
The noxious material, which whether there is the occurrence and development for disease, has more importantly research significance.Although traditional fluorescence is visited
Needle is many kinds of, such as carbon namo fluorescence probe, small organic molecule fluorescence probe and polymer fluorescent nano particle fluorescence probe
Deng, but the size and shape of traditional fluorescence probe is often continuously distributed in wider range, it is difficult to it realizes to fluorescence
The accurate control of probe size and pattern.Meanwhile in complicated biosystem, free fluorescent molecule can meet with aggregation when reuniting
Fluorescent quenching (ACQ) or aggregation-induced emission (AIE) process, to seriously affect the fluorescence intensity of single fluorescent molecule.Due to
Traditional fluorescence probe cannot achieve the accurate dimension and Morphological control to illuminator, it is more difficult to realize the strong light to illuminator
Degree carries out the regulation of quantifying, so traditional fluorescence probe is unable to satisfy modern biotechnology and medical research for fluorescence signal essence
The demand of quasi- quantization needs to develop the scale topography novel nano fluorescence radiation that precisely controllable, fluorescence intensity can precisely quantify
Body.
Summary of the invention
The object of the present invention is to provide customized frame nucleic acid nano illuminator of a kind of shape and preparation method thereof and answer
With, thus solve prior art fluorescence imaging field lack scale topography precisely controllable, fluorescence intensity can precisely quantify it is new
The problem of type nano fluorescent illuminator.
In order to solve the above-mentioned technical problem, the invention adopts the following technical scheme:
According to the first aspect of the invention, a kind of preparation side of customized frame nucleic acid nano illuminator of shape is provided
Method, the preparation method comprises the following steps: S1: by hydrophobic group modification to DNA it is single-stranded on, set by DNA nanostructure
Meter obtains a kind of customized frame nucleic acid nano of shape so that the single-stranded carry out self assembly of DNA that the hydrophobic group is modified
Hydrophobic cavity；And S2: by the frame nucleic acid nano hydrophobic cavity that step S1 is obtained and the hydrophobicity for being dissolved in specific solvent
Fluorescent molecule or hydrophobic drug are mixed according to certain way, so that the hydrophobic fluorescence molecule or hydrophobic drug point
Son enters the frame nucleic acid nano hydrophobic cavity, prepares a kind of customized frame nucleic acid nano illuminator of shape.
Preferably, in step S1, the hydrophobic group includes: that different types of alkyl chain-{ (CH2) x } y-, gallbladder are solid
Alcohol, polycyclic aromatic hydrocarbons (PAH) structure or other molecular structures with hydrophobic property, wherein x is the length of alkyl chain, 1 < x < 100, y
For the item number of alkyl chain, 1 < y < 100.
Preferably, the hydrophobic group is modified on the DNA is single-stranded by the three rouge method of solid phase phosphoramidite of DNA
Any position, including 5 ' ends, 3 ' ends or intermediate any position.
Preferably, the structure of the customized frame nucleic acid nano hydrophobic cavity of the shape includes: DNA tetrahedron, DNA vertical
Cube, DNA trigonal biyramid, DNA octahedron, DNA dodecahedron, DNA icosahedron or DNA paper folding.
Preferably, in step S2, described mix according to certain way includes: any during ultrasound, concussion or shaking table are incubated for
It is a kind of or at least two combination.
Preferably, the hydrophobic fluorescence molecule includes: two area's fluorescent molecule of near-infrared, quantum dot, aggregation-induced emission
Class AIE molecule, Nile red, oil red, hydrophobic cyanine dye, gold nano cluster or containing aromatic hydrocarbon/heterocycle structure fluorescent base
In group any one or at least two combination.
Preferably, the hydrophobic drug includes: that adriamycin, taxol, folic acid, pyrimidine analogue, purine are similar
In object any one or at least two combination.
Preferably, the specific solvent includes: dimethyl sulfoxide, n,N-Dimethylformamide, n,N-dimethylacetamide, third
Ketone, tetrahydrofuran, acetonitrile, acetic acid, dioxane, methanol, ethyl alcohol, isopropanol, hexamethylphosphoramide, fourth sulfone, hydracrylic acid,
Ethamine, ethylenediamine, ethylene glycol, in glycerol any one or at least two combination.
According to the second aspect of the invention, a kind of customized frame core of the shape according to made from above-mentioned preparation method is provided
Sour nano luminous body.
According to the third aspect of the invention we, a kind of customized frame nucleic acid nano illuminator of shape is provided in cell or work
The application of body imaging, and improving the application in hydrophobic drug dissolubility or drug conveying.
The DNA nanotechnology of rising in recent years brings dawn to solve this problem.Height based on DNA sequence dna can be compiled
The building of " atom level " precision nanostructure may be implemented in code property, people.DNA frame nucleic acid is as a kind of DNA nanometers typical
Structure, it not only in the fine morphology and size of nanoscale control structure can should also have good biocompatibility, can
Programmatic, addressability and accurate molecule distinguishability.Therefore, the present invention is based on DNA nanotechnology building shapes to determine
The frame nucleic acid nano hydrophobic cavity and nano luminous body of system not only can use hydrophilic DNA frame nuclease protection inner hydrophobic
Property fluorescent molecule from extraneous bioenvironmental interference, can also be by editor's DNA sequence dna come accuracy controlling DNA frame nucleic acid
Size and shape, thus the hydrophobic fluorescence Molecules of " quantization " regulation nano luminous body assembled, final realization pair
The accurate measurement of fluorescence intensity.
Provided preparation method according to the present invention, as shown in Figure 1, frame nucleic acid nano hydrophobic cavity by hydrophobic inner core with
DNA nanostructure dermoskeleton frame is constituted；By the way that hydrophobic light emitting molecule, nanoparticle or drug and the frame nucleic acid of preparation are received
Rice hydrophobic cavity structural interaction can prepare a kind of customized frame nucleic acid nano illuminator of shape and hydrophobicity medicine
Object carrier.The principle that hydrophobic fluorescent molecule and dewatering medicament molecule enter frame nucleic acid dewatering nano cavity is analogous to organic extraction
The principle taken.When being added in aqueous dna, a part of light emitting molecule can be entered hydrophobic molecule by way of organic extraction
Small luminous cluster is formed inside to hydrophobic cavity.At this point, the outer skeleton of hydrophilic DNA inhibits hydrophobic molecule in aqueous solution
Further aggregation.However in the prior art there is no the external hydrophilic of effectively method building high degree of dispersion, inside is dredged
The nano-sized hydrophobic cavity of water.DNA nano-sized hydrophobic cavity building will consider DNA orientation, incomplementarity DNA sequence dna length etc. because
Element.Secondly, hydrophobic cavity disclosed in the prior art be mostly it is spherical, geometrical morphology and size are simultaneously uncontrollable.For example it utilizes
Liposome, amphiphilic macromolecule etc..But these existing methods can not accurately control the big of material on nanoscale
Small and shape.Such as liposome or cell membrane pack, it is controllable and uniform receive to be difficult to prepare accurate size at present
Rice vesica, and the size of liposome vesicle is mostly in micro-meter scale.However the present invention creatively combines DNA nanotechnology to construct
The frame nucleic acid dewatering nano cavity of a kind of size and structure-controllable, it is spontaneous that this method not only overcomes amphiphile, amphiphilic molecule in solution
Polydispersity when assembled also makes structure have certain shape using the rigidity of DNA structure itself, these are conventional from group
Dress system cannot achieve.
A kind of preparation method of customized frame nucleic acid nano illuminator of shape is provided according to the present invention, it is relatively existing
Technology has following unique advantage: 1) can may customize frame nucleic acid nano by editor's DNA sequence dna come accuracy controlling shape and dredge
The size and shape of water cavity and nano luminous body, so that the hydrophobicity that " quantization " regulation nano fluorescent illuminator is assembled is glimmering
The number of optical molecule, the final accurate measurement realized to fluorescence intensity；2) there is versatility and universality, shape may customize
Nano luminous body can be obtained in frame nucleic acid nano hydrophobic cavity and the assembling of hydrophobic fluorescence molecule, and shape may customize frame core
The good carrier of hydrophobic drug can be obtained in hydrophobic drug assembling for sour nano-sized hydrophobic cavity；3) synthesis quickly, is grasped
Make simply, hydrophobic fluorescence molecule or hydrophobic drug to instrument and equipment without particular/special requirement, therefore have without additional modification
There is certain innovation and practicality.
It is received in conclusion the one kind provided according to the present invention is based on the customizable frame nucleic acid of DNA nanotechnology building shape
It is precisely controllable to not only solve fluorescence imaging field shortage scale topography for the preparation method of rice hydrophobic cavity and nano luminous body
, the novel nano fluorescent illuminant that fluorescence intensity can precisely quantify the problem of, also for hydrophobic drug conveying provide
The customized biological functional carrier of excellent shape provides new grind for biology and the development of the Imaging-PAM of medicine
Study carefully thinking.
Detailed description of the invention
Fig. 1 is the synthesis schematic diagram that shape may customize frame nucleic acid nano hydrophobic cavity and nano luminous body；
Fig. 2 is the single-stranded electrophoresis phenogram of the DNA for the hydrophobic group modification that preparation method according to the present invention obtains,
In band 1 from left to right be that unmodified DNA is single-stranded, band 2 is that the DNA of hydrophobic group modification is single-stranded；
Fig. 3 is the customized frame nucleic acid nano hydrophobic cavity of shape obtained according to a preferred embodiment of the present invention
Electrophoresis phenogram, wherein band 1 from left to right is DNA molecular amount standard, band 2 is frame nucleic acid cube, and band 3 is
The shape that hydrophobic group 1 is modified may customize frame nucleic acid nano hydrophobic cavity V1, and band 4 is the shape that hydrophobic group 2 is modified
Shape may customize frame nucleic acid nano hydrophobic cavity V2, and band 5 is that the shape that hydrophobic group 3 is modified may customize frame nucleic acid nano
Hydrophobic cavity V3, band 6 are that the shape that hydrophobic group 4 is modified may customize frame nucleic acid nano hydrophobic cavity V4；
Fig. 4 A, Fig. 4 B, Fig. 4 C, Fig. 4 D are that shape obtained according to a preferred embodiment of the present invention is customized respectively
The atomic force microscope phenogram (AFM) of frame nucleic acid nano hydrophobic cavity V1, V2, V3, V4；
Fig. 5 A, Fig. 5 B, Fig. 5 C are the tetrahedral framework core of different shapes that preparation method according to the present invention obtains respectively
The structural schematic diagram and atomic force microscope phenogram of sour nano-sized hydrophobic cavity, the knot of cube frame nucleic acid nano hydrophobic cavity
Structure schematic diagram and atomic force microscope phenogram, the structural schematic diagram and atomic force of trigonal biyramid frame nucleic acid nano hydrophobic cavity
Microscopic characterization figure；
Fig. 6 is the fluorescence signal that the shape that preparation method according to the present invention obtains may customize frame nucleic acid nano illuminator
Electrophoresis phenogram, wherein to be that the shape that hydrophobic group 2 is modified may customize frame nucleic acid nano hydrophobic for band 1 from left to right
Cavity, band 2 be hydrophobic group 2 modify shape may customize frame nucleic acid nano illuminator (frame nucleic acid structure be cube
Body, hydrophobic fluorescence molecule are aggregation-induced emission class AIE fluorescent molecule)；
Fig. 7 is the fluorescence spectrum that the shape that preparation method according to the present invention obtains may customize frame nucleic acid nano illuminator
Figure, sample are that the shape that hydrophobic group 2 is modified may customize frame nucleic acid nano illuminator, wherein frame nucleic acid structure is vertical
Cube, hydrophobic fluorescence molecule are aggregation-induced emission class AIE fluorescent molecule；
Fig. 8 is the electricity that the shape obtained according to the method for the present invention may customize the fluorescence signal of frame nucleic acid nano illuminator
Swimming phenogram, wherein hydrophobic group is hydrophobic group 4, and DNA frame nucleic acid is cube, and hydrophobic fluorescence molecule is oil
It is red；
Fig. 9 A, Fig. 9 B, Fig. 9 C are the frame nucleic acid structure obtained according to the method for the present invention, the customizable frame of shape respectively
Nucleic acid nano hydrophobic cavity and shape may customize the atomic force microscope phenogram (AFM) of frame nucleic acid nano illuminator；
Figure 10 is the frame nucleic acid structure obtained according to the method for the present invention, the customizable hydrophobic sky of frame nucleic acid nano of shape
The particle height that chamber and shape may customize the atomic force microscope phenogram of frame nucleic acid nano illuminator counts box traction substation,
In from left to right be respectively frame nucleic acid structure cube, hydrophobic group 4 modify shape may customize frame nucleic acid nano dredge
The shape that water cavity (frame nucleic acid structure is cube), hydrophobic group 4 are modified may customize frame nucleic acid nano illuminator (frame
Frame nucleic acid structure is cube, and hydrophobic fluorescence molecule is oil red).
Specific embodiment
Below in conjunction with the specific embodiment of the application, the technical solution of the application is described in detail, but such as
Lower embodiment is only that and cannot limit the application to understand the application, the features in the embodiments and the embodiments of the present application
It can be combined with each other, the multitude of different ways that the application can be defined by the claims and cover is implemented.
Embodiment 1
The one kind provided according to the present invention is based on DNA nanotechnology and constructs the customized frame nucleic acid nano illuminator of shape
Preparation method, the present embodiment synthesized four kinds of shapes customized frame nucleic acid nano hydrophobic cavity V1, V2, V3, V4, including
Following steps:
Respectively in 5 ' the end modified upper hydrophobic groups 1 of the single-stranded B ' of DNA single-stranded A ' and DNA, wherein hydrophobic group 1
For-{ (CH2) 12 } 3-.All DNA are dissolved in Q water, ultraviolet specrophotometer is quantified.
Polyacrylamide gel electrophoresis (Fig. 2) the result shows that, hydrophobic group modification DNA it is single-stranded than unmodified DNA
Single-stranded migration rate is slack-off, this is because hydrophobic group has been modified, caused by DNA single-stranded molecular weight becomes larger.
Then, clip1, clip2, clip3 are taken, the DNA single-stranded A ' and B ' that clip4 and hydrophobic group 1 are modified is dissolved in 100
In the TAMg buffer solution of μ L, the single-stranded molar ratio of DNA is 1:1:1:1:4:4, wherein TAMg buffer solution is that magnesium ion concentration is
The Tris buffer salt solution of 12.5mM.After mixing, reaction system is placed in PCR instrument and is reacted, hydrophobicity can be obtained
The customized frame nucleic acid nano hydrophobic cavity V1 of shape that group 1 is modified.Wherein, cycle of annealing be 95 degrees Celsius 5 minutes, 80
Degrees Celsius 3 minutes, then from 80 degrees Celsius of gradient coolings to 4 degrees Celsius in 200 minutes.
Similarly, by hydrophobic group 1 be changed to hydrophobic group 2 (- { (CH2) 18 } 3-), hydrophobic group 3 (-
{ (CH2CH2-O) 4-Cholesterol } 3-), hydrophobic group 4 (- { (CH2) 12 } 4-), other methods remain unchanged
Obtaining the shape that the shape that hydrophobic group 2 is modified may customize frame nucleic acid nano hydrophobic cavity V2, hydrophobic group 3 is modified can determine
The shape of frame nucleic acid nano hydrophobic cavity V3 and hydrophobic group 4 modification processed may customize frame nucleic acid nano hydrophobic cavity
V4.After high-efficient liquid phase chromatogram purification, the customized frame nucleic acid nano hydrophobic cavity of gained shape can be through polyacrylamide
Gel electrophoresis and atomic force microscope (AFM) characterization.
Polyacrylamide gel electrophoresis (Fig. 3) the result shows that, shape may customize frame nucleic acid nano hydrophobic cavity synthesis at
Function, and compared with unmodified frame nucleic acid structure, the shape that hydrophobic group 1 and hydrophobic group 2 are modified may customize frame
Frame nucleic acid nano hydrophobic cavity V1, V2 migration rate is slack-off, and the shape that hydrophobic group 3 and hydrophobic group 4 are modified can determine
Frame nucleic acid nano hydrophobic cavity V3, V4 migration rate processed becomes faster, and illustrates that modifying different hydrophobic groups may customize shape
The migration rate of frame nucleic acid nano hydrophobic cavity influences different.
Atomic force microscope (Fig. 4 A, Fig. 4 B, Fig. 4 C, Fig. 4 D) the result shows that, the shape that hydrophobic group 1 is modified is customizable
The shape that frame nucleic acid nano hydrophobic cavity V1, hydrophobic group 2 are modified may customize frame nucleic acid nano hydrophobic cavity V2, hydrophobic
Property group 3 modify shape may customize frame nucleic acid nano hydrophobic cavity V3, hydrophobic group 4 modify shape may customize frame
Nucleic acid nano hydrophobic cavity V4 is uniform complete cube structure.
Wherein, the DNA of hydrophobic group modification is single-stranded biological (Shanghai) purchased from outstanding Lee；Remaining reagent is purchased from Sigma's Order
Ritchie China；PCR instrument is BIO-RADT100PCR instrument；High performance liquid chromatograph is AgilentTechnologies
1260Infinity instrument；Atomic force microscope is Brukermultimode 8.
DNA sequence dna is as follows:
Wherein, X=(- CH2CH2-O-) 6-OP (O2) O-).
It should be understood that herein only by way of example and not limitation, it is suitable for the invention frame nucleic acid structure and not only
It is limited to above-mentioned sequence.
Embodiment 2
What is provided according to the present invention may customize frame nucleic acid nano hydrophobic cavity and is received based on DNA nanotechnology building shape
The preparation method of rice illuminator, synthesizing shape of different shapes in the present embodiment may customize frame nucleic acid nano hydrophobic cavity,
The following steps are included:
It is dissolved in all DNA are single-stranded in deionized water, is quantified with ultraviolet specrophotometer.
S1, S2, S3 are taken, S4 chain is dissolved in the TM buffer solution of 100 μ L, and the single-stranded molar ratio of DNA is 1:1:1:1, wherein TM
Buffer solution is the Tris buffer salt solution that magnesium ion concentration is 5mM.After mixing, reaction system is placed in PCR instrument
Reaction, can be obtained tetrahedral framework nucleic acid nano hydrophobic cavity.Wherein, cycle of annealing be 95 degrees Celsius 15 minutes, 4 degrees Celsius
15 minutes.After high-efficient liquid phase chromatogram purification, gained tetrahedral framework nucleic acid nano hydrophobic cavity can be through atomic force microscope
(AFM) it characterizes.
Take clip1, clip2, clip3, clip4, A ' and B ' chain be dissolved in the TAMg buffer solution of 100 μ L, DNA is single-stranded to rub
You are than being 1:1:1:1:4:4, wherein TAMg buffer solution is the Tris buffer salt solution that magnesium ion concentration is 12.5mM.By
After mixing, reaction system is placed in PCR instrument and is reacted, cube frame nucleic acid nano hydrophobic cavity can be obtained.Wherein, it moves back
Fiery program be 95 degrees Celsius 5 minutes, 80 degrees Celsius 3 minutes, it is then Celsius to 4 from 80 degrees Celsius of gradient coolings in 200 minutes
Degree.After high-efficient liquid phase chromatogram purification, gained cube frame nucleic acid nano hydrophobic cavity can be through atomic force microscope (AFM)
Characterization.
A, b, c, A, B are taken, C chain is dissolved in the TM buffer solution of 100 μ L, and the single-stranded molar ratio of DNA is 1:1:1:1:1:1,
In, TM buffer solution is the Tris buffer salt solution that magnesium ion concentration is 5mM.After mixing, reaction system is placed in PCR
It is reacted in instrument, trigonal biyramid frame nucleic acid nano hydrophobic cavity can be obtained.Wherein, cycle of annealing be 95 degrees Celsius 3 minutes, so
Afterwards from 95 degrees Celsius of gradient coolings to 25 degrees Celsius in 15 minutes.After high-efficient liquid phase chromatogram purification, gained trigonal biyramid
Frame nucleic acid nano hydrophobic cavity can be characterized through atomic force microscope (AFM).
Atomic force microscope phenogram (Fig. 5 A, Fig. 5 B, Fig. 5 C) shows tetrahedral framework nucleic acid nano hydrophobic cavity, stands
Cube frame nucleic acid nano hydrophobic cavity and trigonal biyramid frame nucleic acid nano hydrophobic cavity can form expected structure.
Wherein, the DNA of hydrophobic group modification is single-stranded biological (Shanghai) purchased from outstanding Lee；Remaining reagent is purchased from Sigma's Order
Ritchie China；PCR instrument is BIO-RADT100PCR instrument；High performance liquid chromatograph is AgilentTechnologies
1260Infinity instrument；Atomic force microscope is Brukermultimode 8.
DNA sequence dna is as follows:
Clip1 described in this implementation, clip2, clip3, clip4, A ', B ' chain-ordering is the same as the DNA provided in embodiment 1
Sequence is consistent, does not repeat to provide herein.
It should be understood that herein only by way of example and not limitation, it is suitable for the invention frame nucleic acid structure and not only
It is limited to above-mentioned sequence.
Embodiment 3
The shape that hydrophobic group 2 is modified may customize frame nucleic acid nano hydrophobic cavity and assemble aggregation-induced emission class AIE
Molecule, which forms shape, may customize frame nucleic acid nano illuminator, comprising the following steps:
Aggregation-induced emission class AIE molecule is added to the customizable frame nucleic acid nano of shape that hydrophobic group 2 is modified to dredge
In water cavity system, wherein aggregation-induced emission class AIE molecule is significantly excessive.By concussion or ultrasound by AIE molecule with it is hydrophobic
Property the shape modified of group 2 may customize frame nucleic acid nano hydrophobic cavity and mix, enter AIE molecule by hydrophobic forces thin
The shape that aqueous group 2 is modified may customize in frame nucleic acid nano hydrophobic cavity, ultimately form the shape of the modification of hydrophobic group 2
Customizable frame nucleic acid nano illuminator.
To hydrophobic group 2 modify shape may customize frame nucleic acid nano illuminator in be added appropriate 60% sucrose it is molten
Liquid may customize frame nucleic acid nano illuminator with agarose electrophoresis characterization shape.Wherein, the effect of sucrose solution is loading buffer
Liquid.Buffer used in agarose electrophoresis is TAE-Mg solution, and 1LTAE-Mg solution includes Tris buffer salt 40mM, EDTA-Na2
2mM, MgAc4H2O12.5mM, acetic acid 1.14ml.It should be noted that any fluorescent core acid cure is not added in agarose gel electrophoresis
Glue staining reagent.It, can with the shape that SyngeneGBOXChemi luminescence imaging instrument sign hydrophobic group 2 is modified after electrophoresis
Customize frame nucleic acid nano illuminator.It should be noted that can visually observe directly group under the ultraviolet light irradiation of 365nm
The customizable frame nucleic acid nano illuminator of shape that the hydrophobic group 2 of AIE molecule is modified is filled.
Agarose gel figure (Fig. 6) show hydrophobic group modification shape may customize frame nucleic acid nano hydrophobic cavity with
AIE molecule assembles to form uniform band, i.e., the shape that hydrophobic group 2 is modified may customize frame nucleic acid nano illuminator
It synthesizes successfully.As seen from Figure 6, the shape that do not modify with the hydrophobic group 2 of AIE molecule assembling may customize frame nucleic acid and receive
Rice hydrophobic cavity is shown when fluorescence nucleic acid gel staining reagent is not added without DNA band, is the shape that hydrophobic group 2 is modified
Shape may customize the negative control of frame nucleic acid nano illuminator.And after being the assembling of AIE molecule, the shape of the modification of hydrophobic group 2
Customizable frame nucleic acid nano illuminator can then show the fluorescence signal of AIE molecule.Fig. 7 is that the shape may customize frame nucleic acid
The fluorescence spectra of nano luminous body, wherein a length of 367 nanometers of excitation light wave.The result shows that the shape may customize frame nucleic acid
Nano luminous body has high fluorescence intensity.
Embodiment 4
The shape that hydrophobic group 4 is modified may customize frame nucleic acid nano hydrophobic cavity assembling oil red molecule formation shape can
Customize frame nucleic acid nano illuminator, comprising the following steps:
The shape that oil red molecule (SLBH0251V is purchased from SIGMA-ALDRICH) is added to the modification of hydrophobic group 4 can
It customizes in frame nucleic acid nano hydrophobic cavity system, the shape for being modified oil red molecule and hydrophobic group 4 by concussion or ultrasound
Shape may customize frame nucleic acid nano hydrophobic cavity and mix, and so that oil red molecule is entered hydrophobic group 4 by hydrophobic forces and modifies
Shape may customize frame nucleic acid nano hydrophobic cavity in, ultimately form hydrophobic group 4 modification shape may customize frame core
Sour nano luminous body.
To hydrophobic group 4 modify shape may customize frame nucleic acid nano illuminator in be added appropriate 60% sucrose it is molten
Liquid may customize frame nucleic acid nano illuminator with agarose electrophoresis characterization shape.Wherein, the effect of sucrose solution is loading buffer
Liquid, buffer used in agarose electrophoresis are TAE-Mg solution.It should be noted that any fluorescent core is not added in agarose gel electrophoresis
Acid gel staining reagent.After electrophoresis, with the shape of the SyngeneGBOXChemi luminescence imaging instrument sign modification of hydrophobic group 4
Shape may customize frame nucleic acid nano illuminator.It should be noted that naked eyes are i.e. it can be seen that red assembles dredging for oil red molecule
The shape that aqueous group 4 is modified may customize frame nucleic acid nano illuminator.
Agarose gel figure (Fig. 8) shows that the shape of hydrophobic group modification may customize frame nucleic acid nano hydrophobic cavity and oil
Red molecule assembles to form uniform band, i.e., the shape that hydrophobic group 4 is modified may customize frame nucleic acid nano illuminator
It synthesizes successfully.The shape that Fig. 9 display frame nucleic acid cube structure, hydrophobic group 4 are modified may customize frame nucleic acid nano and dredge
The shape that water cavity, hydrophobic group 4 are modified may customize frame nucleic acid nano illuminator (cube assembles oil red molecule) all
For uniform complete cube structure.And by the height (Figure 10) of Statistical Comparison particle it can be seen that hydrophobic group 4 is modified
Shape may customize frame nucleic acid nano hydrophobic cavity highly significant be less than frame nucleic acid structure, illustrate hydrophobic group in frame
Nano-sized hydrophobic cavity is formd inside frame nucleic acid structure, and the presence of nano-sized hydrophobic cavity can allow the structure of frame nucleic acid to have centainly
The collapse of degree causes height to reduce.And it assembles the shape after oil red molecule and may customize frame nucleic acid nano illuminator
Height significantly increases compared with the shape that hydrophobic group 4 is modified may customize frame nucleic acid nano hydrophobic cavity, illustrates oil red point
After son enters nano-sized hydrophobic cavity, nano-sized hydrophobic cavity can be strutted to a certain extent, so that shape be made to may customize frame nucleic acid
The height of nano luminous body increases.
Above-described, only presently preferred embodiments of the present invention, the range being not intended to limit the invention, of the invention is upper
Stating embodiment can also make a variety of changes.Letter made by all claims applied according to the present invention and description
Single, equivalent changes and modifications, fall within the claims of the invention patent.The not detailed description of the present invention is normal
Advise technology contents.
Sequence table
<110>Shanghai Communications University
<120>customized frame nucleic acid nano illuminator of a kind of shape and its preparation method and application
<160> 16
<210> 1
<211> 19
<212> DNA
<213>artificial sequence
<220> A'
<223>outstanding Lee is biological (Shanghai)
<400> 1
tttttcagtt gaccatata 19
<210> 2
<211> 19
<212> DNA
<213>artificial sequence
<220> B'
<223>outstanding Lee is biological (Shanghai)
<400> 2
ttttaccatc tggtattac 19
<210> 3
<211> 84
<212> DNA
<213>artificial sequence
<220> clip1
<223>outstanding Lee is biological (Shanghai)
<400> 3
tcgctgagta xtcctatatg gtcaactgct cxgcaagtgt gggcacgcac acxgtagtaa 60
taccagatgg agtxcacaaa tctg 84
<210> 4
<211> 84
<212> DNA
<213>artificial sequence
<220> clip2
<223>outstanding Lee is biological (Shanghai)
<400> 4
ctatcggtag xtcctatatg gtcaactgct cxtactcagc gacagatttg tgxgtagtaa 60
taccagatgg agtxcaacta gcgg 84
<210> 5
<211> 84
<212> DNA
<213>artificial sequence
<220> clip3
<223>outstanding Lee is biological (Shanghai)
<400> 5
cactggtcag xtcctatatg gtcaactgct cxctaccgat agccgctagt tgxgtagtaa 60
taccagatgg agtxggtttg ctga 84
<210> 6
<211> 84
<212> DNA
<213>artificial sequence
<220> clip4
<223>outstanding Lee is biological (Shanghai)
<400> 6
ccacacttgc xtcctatatg gtcaactgct cxctgaccag tgtcagcaaa ccxgtagtaa 60
taccagatgg agtxgtgtgc gtgc 84
<210> 7
<211> 63
<212> DNA
<213>artificial sequence
<220> S1
<223>outstanding Lee is biological (Shanghai)
<400> 7
acgtatcacc aggcagttga gacgaacatt cctaagtctg aaatttatca cccgccatag 60
tag 63
<210> 8
<211> 63
<212> DNA
<213>artificial sequence
<220> S2
<223>outstanding Lee is biological (Shanghai)
<400> 8
acgattacag cttgctacac gattcagact taggaatgtt cgacttgcgt gggtccaata 60
ccg 63
<210> 9
<211> 63
<212> DNA
<213>artificial sequence
<220> S3
<223>outstanding Lee is biological (Shanghai)
<400> 9
ggtgataaaa cgtgtagcaa gctgtaatcg acgggatgag catgcccttc cactactatg 60
gcg 63
<210> 10
<211> 63
<212> DNA
<213>artificial sequence
<220> S4
<223>outstanding Lee is biological (Shanghai)
<400> 10
ccacgcaaga ctcaactgcc tggtgatacg aggaagggca tgctcatccc gacggtattg 60
gac 63
<210> 11
<211> 63
<212> DNA
<213>artificial sequence
<220> a
<223>outstanding Lee is biological (Shanghai)
<400> 11
aggcagttga gacgaacatt cctaagtctg aaatttatca cccgccatag tagacgtatc 60
acc 63
<210> 12
<211> 63
<212> DNA
<213>artificial sequence
<220> b
<223>outstanding Lee is biological (Shanghai)
<400> 12
cttgctacac gattcagact taggaatgtt cgacatgcga gggtccaata ccgacgatta 60
cag 63
<210> 13
<211> 63
<212> DNA
<213>artificial sequence
<220> c
<223>outstanding Lee is biological (Shanghai)
<400> 13
ggtgataaaa cgtgtagcaa gctgtaatcg acgggaagag catgcccatc cactactatg 60
gcg 63
<210> 14
<211> 63
<212> DNA
<213>artificial sequence
<220> A
<223>outstanding Lee is biological (Shanghai)
<400> 14
ctgcgcggat gactcaactg cctggtgata cgatctagtc tctacgtcaa gtaagaacct 60
tag 63
<210> 15
<211> 63
<212> DNA
<213>artificial sequence
<220> B
<223>outstanding Lee is biological (Shanghai)
<400> 15
cctcgcatga catccgcgca gctaaggttc aaagttcctg ccgcttcacg gacggtattg 60
gac 63
<210> 16
<211> 63
<212> DNA
<213>artificial sequence
<220> C
<223>outstanding Lee is biological (Shanghai)
<400> 16
ctcttcccga ccgtgaagcg gcaggaactt atacttgacg tagagactag aaggatgggc 60
atg 63
Claims (10)
1. a kind of preparation method of the customized frame nucleic acid nano illuminator of shape, which is characterized in that the preparation method packet
Include following steps:
S1: by hydrophobic group modification to DNA it is single-stranded on, by DNA nanostructure design so that the hydrophobic group modify
The single-stranded carry out self assembly of DNA, obtain a kind of customized frame nucleic acid nano hydrophobic cavity of shape；And
S2: by the frame nucleic acid nano hydrophobic cavity that step S1 is obtained and the hydrophobic fluorescence molecule for being dissolved in specific solvent or
Hydrophobic drug is mixed according to certain way, so that described in the hydrophobic fluorescence molecule or hydrophobic drug entrance
Frame nucleic acid nano hydrophobic cavity prepares a kind of customized frame nucleic acid nano illuminator of shape.
2. preparation method according to claim 1, which is characterized in that in step S1, the hydrophobic group includes: difference
Alkyl chain-{ (the CH of type2)x}y, cholesterol, polycyclic aromatic hydrocarbons (PAH) structure or other molecular structures with hydrophobic property,
In, x is the length of alkyl chain, and 1 < x < 100, y are the item number of alkyl chain, 1 < y < 100.
3. preparation method according to claim 1, which is characterized in that the hydrophobic group passes through the solid phase phosphorous of DNA
Three rouge method of amide modifies any position on the DNA is single-stranded, including 5 ' ends, 3 ' ends or intermediate any position.
4. preparation method according to claim 1, which is characterized in that the customized frame nucleic acid nano of shape is hydrophobic
The structure of cavity includes: DNA tetrahedron, DNA cube, DNA trigonal biyramid, DNA octahedron, DNA dodecahedron, DNA 20
Face body or DNA paper folding.
5. preparation method according to claim 1, which is characterized in that in step S2, described mix according to certain way is wrapped
Include: ultrasound, concussion or shaking table be incubated in any one or at least two combination.
6. preparation method according to claim 1, which is characterized in that the hydrophobic fluorescence molecule includes: near-infrared two
Area's fluorescent molecule, quantum dot, aggregation-induced emission class AIE molecule, Nile red, oil red, hydrophobic cyanine dye, gold nano group
Cluster or containing in aromatic hydrocarbon/heterocycle structure fluorophor any one or at least two combination.
7. preparation method according to claim 1, which is characterized in that the hydrophobic drug includes: adriamycin, purple
China fir alcohol, folic acid, pyrimidine analogue, in purine analogue any one or at least two combination.
8. preparation method according to claim 1, which is characterized in that the specific solvent includes: dimethyl sulfoxide, N, N- bis-
Methylformamide, n,N-dimethylacetamide, acetone, tetrahydrofuran, acetonitrile, acetic acid, dioxane, methanol, ethyl alcohol, isopropyl
Alcohol, hexamethylphosphoramide, fourth sulfone, hydracrylic acid, ethamine, ethylenediamine, ethylene glycol, in glycerol any one or at least two
Combination.
9. the customized frame nucleic acid of shape made from a kind of preparation method described in any one of -8 according to claim 1 is received
Rice illuminator.
10. a kind of application of the customized frame nucleic acid nano illuminator of shape as claimed in claim 9, which is characterized in that packet
It includes: in cell imaging, living imaging, improving hydrophobic drug dissolubility and improve the application in hydrophobic drug conveying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910589495.9A CN110257053B (en) | 2019-07-02 | 2019-07-02 | Shape-customizable framework nucleic acid nano-luminophor and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910589495.9A CN110257053B (en) | 2019-07-02 | 2019-07-02 | Shape-customizable framework nucleic acid nano-luminophor and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110257053A true CN110257053A (en) | 2019-09-20 |
| CN110257053B CN110257053B (en) | 2021-01-15 |
Family
ID=67923768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910589495.9A Active CN110257053B (en) | 2019-07-02 | 2019-07-02 | Shape-customizable framework nucleic acid nano-luminophor and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110257053B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111671920A (en) * | 2020-06-22 | 2020-09-18 | 南开大学 | Preparation and application of cyanine photosensitizer nucleic acid nano-aggregate |
| CN111690030A (en) * | 2020-06-19 | 2020-09-22 | 湖南大学 | Single-stranded DNA, G-tetramer and preparation method and application of G-tetramer |
| CN111825734A (en) * | 2020-07-21 | 2020-10-27 | 山东大学 | Two-branch DNA tetrahedral nano structure and synthetic method and application thereof |
| CN112156104A (en) * | 2020-11-10 | 2021-01-01 | 四川大学 | Medicine for treating depression |
| CN112326762A (en) * | 2020-10-29 | 2021-02-05 | 常州大学 | Method for detecting DNA tetrahedron by capillary electrophoresis |
| CN113777034A (en) * | 2021-08-20 | 2021-12-10 | 嘉兴学院 | Gold nanometer bipyramid array substrate and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109196103A (en) * | 2016-05-10 | 2019-01-11 | 俄亥俄州立创新基金会 | The 3D RNA cage type nanoparticle of self assembly |
| CN109568595A (en) * | 2019-01-23 | 2019-04-05 | 上海交通大学 | Nucleic acid-drug conjugates, drug delivery system and its preparation method and application |
-
2019
- 2019-07-02 CN CN201910589495.9A patent/CN110257053B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109196103A (en) * | 2016-05-10 | 2019-01-11 | 俄亥俄州立创新基金会 | The 3D RNA cage type nanoparticle of self assembly |
| CN109568595A (en) * | 2019-01-23 | 2019-04-05 | 上海交通大学 | Nucleic acid-drug conjugates, drug delivery system and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| EDWARDSON THOMAS G. W.等: "Site-specific positioning of dendritic alkyl chains on DNA cages enables their geometry-dependent self-assembly", 《NATURE CHEMISTRY》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111690030A (en) * | 2020-06-19 | 2020-09-22 | 湖南大学 | Single-stranded DNA, G-tetramer and preparation method and application of G-tetramer |
| CN111690030B (en) * | 2020-06-19 | 2021-12-10 | 湖南大学 | Single-stranded DNA, G-tetramer and preparation method and application of G-tetramer |
| CN111671920A (en) * | 2020-06-22 | 2020-09-18 | 南开大学 | Preparation and application of cyanine photosensitizer nucleic acid nano-aggregate |
| CN111671920B (en) * | 2020-06-22 | 2022-12-06 | 南开大学 | Preparation and application of cyanine photosensitizer nucleic acid nano-aggregate |
| CN111825734A (en) * | 2020-07-21 | 2020-10-27 | 山东大学 | Two-branch DNA tetrahedral nano structure and synthetic method and application thereof |
| CN112326762A (en) * | 2020-10-29 | 2021-02-05 | 常州大学 | Method for detecting DNA tetrahedron by capillary electrophoresis |
| CN112326762B (en) * | 2020-10-29 | 2023-03-21 | 常州大学 | Method for detecting DNA tetrahedron by capillary electrophoresis |
| CN112156104A (en) * | 2020-11-10 | 2021-01-01 | 四川大学 | Medicine for treating depression |
| CN112156104B (en) * | 2020-11-10 | 2021-12-31 | 四川大学 | Medicine for treating depression |
| CN113777034A (en) * | 2021-08-20 | 2021-12-10 | 嘉兴学院 | Gold nanometer bipyramid array substrate and preparation method and application thereof |
| CN113777034B (en) * | 2021-08-20 | 2024-04-19 | 嘉兴学院 | Gold nano bipyramid array substrate and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110257053B (en) | 2021-01-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110257053A (en) | A kind of customized frame nucleic acid nano illuminator of shape and its preparation method and application | |
| Ueda et al. | Tissue clearing and its applications in neuroscience | |
| Lou et al. | Organic dots based on AIEgens for two‐photon fluorescence bioimaging | |
| Zrazhevskiy et al. | Multifunctional quantum dots for personalized medicine | |
| Wang et al. | Targeted imaging and targeted therapy of breast cancer cells via fluorescent double template-imprinted polymer coated silicon nanoparticles by an epitope approach | |
| CN105802611B (en) | A kind of Ratio-type nano silicon quantum dots fluorescence probe and its preparation method and application | |
| CN105295909A (en) | Method for using phenylenediamine and citric acid for preparing carbon quantum dot marking probe for cell developing | |
| CN106587005A (en) | Multi-step carbonized high quantum efficiency carbon quantum dot and preparation method thereof | |
| He et al. | Visible and near-infrared dual-emission carbogenic small molecular complex with high RNA selectivity and renal clearance for nucleolus and tumor imaging | |
| CN109395079B (en) | Multifunctional nano probe and preparation method and application thereof | |
| CN106620725B (en) | Optical and photoacoustic integrated bimodal molecular imaging probe and preparation method and application thereof | |
| Zhou et al. | The in vivo targeted molecular imaging of fluorescent silicon nanoparticles in Caenorhabditis elegans | |
| US20190224318A1 (en) | Preparation and Application of Novel Multifunctional Nanocomposite Material with new Photosensitizer | |
| CN101805362A (en) | Porphyrin modified by diethylenetriamine pentaacetic acid gadolinium and preparation method and application thereof | |
| Zhang et al. | Near-infrared BODIPY-paclitaxel conjugates assembling organic nanoparticles for chemotherapy and bioimaging | |
| Du et al. | Encapsulation-Dependent Enhanced Emission of Near-Infrared Nanoparticles Using in Vivo Three-Photon Fluorescence Imaging | |
| Li et al. | Asymmetrically coating Pt nanoparticles on magnetic silica nanospheres for target cell capture and therapy | |
| Liu et al. | Triple-emitting dumbbell fluorescent nanoprobe for multicolor detection and imaging applications | |
| Ma et al. | Aptamer-modified carbon dots for enhancement of photodynamic therapy of cancer cells | |
| CN111999504B (en) | Mucin 1 and sialylglycosyl dual fluorescence imaging method and application thereof | |
| Gong et al. | A NIR light gated targeting nanoprobe based on DNA-modified upconversion nanoparticles with antifouling properties for ratiometric detection and imaging of microRNA-21 | |
| Yang et al. | Lifetime-engineered ruby nanoparticles (tau-rubies) for multiplexed imaging of μ-opioid receptors | |
| Li et al. | Long-term real-time tracking live stem cells/cancer cells in vitro/in vivo through highly biocompatible photoluminescent poly (citrate-siloxane) nanoparticles | |
| CN105158125B (en) | A kind of telomere length measuring method | |
| CN106512029A (en) | Nano-probe with apoptosis target function and preparation and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |