CN110255703A - A kind of fast culture process of aerobic ammonia oxidation granule sludge - Google Patents
A kind of fast culture process of aerobic ammonia oxidation granule sludge Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/00—Biological treatment of water, waste water, or sewage
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Abstract
A kind of method of the fast culture of aerobic ammonia oxidation granule sludge, the aerobic particle mud based on heterotroph is first inoculated with into sequencing batch reactor as carrier, by gradually reducing influent COD value to organic concentration, to be zero tame aerobic ammonia oxidation granule sludge again, and the fast enriching for adding High-efficient Nitrobacteria concentrate during domestication to promote autotrophic bacterium in granule sludge, fast culture go out aerobic ammonia oxidation granule sludge.The present invention makes full use of the bio-carrier of heterotrophism granule sludge to act on, the slow disadvantage of nitrobacteria multiplication rate is made up by adding external source aerobic ammonia oxidation bacterium, realize the fast enriching of aerobic ammonia oxidation bacterium in heterotrophism granule sludge, the advantages such as the settling property for giving full play to AGS is good, high resistance to poison, provide technical support for waste water innoxious governance.The present invention can effectively reduce impact of the drastic change of service condition to inoculation AGS stability, realize that heterotroph is by the replacement of aerobic ammonia oxidation bacterium in particle in a short time.
Description
Technical field
The present invention relates to technical field of biological sewage treatment.
Background technique
Aerobic particle mud (aerobic granular sludge, AGS) is that microorganism is spontaneous solidifying under particular circumstances
There is good compact structure, settling property, high resistance to poison, simultaneous denitrification to remove for poly-, proliferation and the particular biological condensate formed
The advantages that phosphorus.Facts have proved: compared to traditional activated sludge process, AGS technology can substantially reduce construction investment and operating cost, right
Poisonous and harmful waste water shows good removal effect.According to the type of utilization carbon source, AGS can be divided into heterotrophism granule sludge and oneself
Support granule sludge.Wherein, due to Autotrophic nitrification granule sludge has good Nitrification and stability and by favor, it
Occur providing a kind of new approaches for the improvement of south jiangxi Rare-earth Mine waste water.Unfortunately, due to the proliferation that nitrobacteria is extremely low
Rate causes the self-coagulation between them very slow.Studies have shown that Autotrophic nitrification particle is dirty using activated sludge as seed sludge
The formation time of mud often at two months or more, even up to the several years, greatly limits the development and popularization of technology.Currently, inoculation
Part AGS accelerates the storage of aerobic granular process and AGS and fast quick-recovery many cases occur, this shows that AGS is
A kind of good bio-carrier, and can be used as the medium of specific function bacterium growth enrichment.In addition, heterotrophism granule sludge has been achieved with
Engineering application is easier to obtain relative to Autotrophic nitrification granule sludge, and has been demonstrated that inoculation heterotrophism granule sludge can add
The formation of fast Autotrophic nitrification granule sludge.Unfortunately, it directlys adopt inorganic wastewater and tames autotrophy nitre by heterotrophism granule sludge
Change disintegration and function bacterium leakage that granule sludge is easy to appear particle, needs strict control to run when causing using the strategy
Can condition be applied and still need to examine.
Presently disclosed document mainly have the preparation of aerobic nitrifying granular activated sludge of Publication No. CN101386448 a kind of with
Restorative procedure, a kind of aerobic nitrifying granular activated sludge long-term preservation and method for activation recovering of Publication No. CN103880168A are public
The number of opening is the method using anaerobic grain sludge culture nitrifying granular activated sludge of CN1821122, Publication No. CN102040281A
A kind of nitrogen removal granule sludge cultural method, a kind of joint FA and FNA the double inhibition training of Publication No. CN102173504A
The method for supporting short distance nitration granule sludge, a kind of processing sanitary sewage aerobic short-cut nitrification granular of Publication No. CN101531983
The cultural method of sludge, a kind of heterotrophic nitrification aerobic granule sludge and its cultural method and use of Publication No. CN101306870
On the way, the cultural method of a kind of aerobic nitrosated granular sludge of Publication No. CN1699217, Publication No. CN104529056A's
A kind of method of floc sludge and granule sludge symbiosis realization municipal sewage autotrophic denitrification, the one of Publication No. CN103922466A
The nitrosated granular sludge cultural method of kind of room temperature processing city domestic sewage, Publication No. CN103663725A based on particle
The continuous flow biological denitrification method and device of sludge, but have no that adding external source nitrobacteria promotes Autotrophic nitrification granule sludge quick
The technical solution of formation.
Summary of the invention
It is too long the purpose of the present invention is to solve Autotrophic nitrification granule sludge incubation time and be difficult to fast implement short distance
The problems such as nitrification, provides a kind of fast culture process of aerobic ammonia oxidation granule sludge.
The purpose of the present invention is achieved through the following technical solutions:
A kind of fast culture process of aerobic ammonia oxidation granule sludge, this method be first be inoculated with it is aerobic based on heterotroph
Granule sludge is used as carrier into sequencing batch reactor, then to be zero tame and docile by gradually reducing influent COD value to organic concentration
Change aerobic ammonia oxidation granule sludge, and adds High-efficient Nitrobacteria concentrate during domestication to promote autotrophic bacterium in particle dirt
Fast enriching in mud, fast culture go out aerobic ammonia oxidation granule sludge.
Inoculation aerobic particle mud of the present invention, autotrophic bacterium ratio≤10%, sinking speed >=15m/h are seeded to
MLSS >=3000mg/L after reactor.The sequencing batch reactor is cylindrical reactor, and ratio of height to diameter is 4~20, and reactor is heavy
Time control drops within 5 minutes, and the apparent gas velocity that rises is in 1.2cm/s or more.The water inlet is in domestication aerobic ammonia oxidation particle
Inorganic high ammonia-nitrogen wastewater is gradually converted by organic sewage in sludge process, which provides carbon by sodium bicarbonate
Source, between 60~320mg/L of influent ammonia nitrogen, pH 6-9.The High-efficient Nitrobacteria concentrate is commercially available cleaning aquarium
Nitrobacteria concentrate, contained bacterium are aerobic ammonia oxidation bacterium, and the active nitrobacteria quantity of every milliliter of bacterium solution is greater than 300,000,000
It is a.High-efficient Nitrobacteria concentrate minimum dosage is 1 milliliter of bacterium solution/liter reactor mixed liquor, and adding the time continues to reaction
Until the water outlet nitrite nitrogen accumulation rate of device is more than 90%.The aerobic ammonia oxidation granule sludge tames Success Flag are as follows: particle
The SVI of sludge is maintained at 50mL/g hereinafter, particle rate is maintained at 90% or more, nitrite nitrogen accumulation rate and to influent ammonia nitrogen
Removal rate is held in 90% or more.The dominant bacteria of heterotroph in the anaerobic ammonium oxidation granule sludge is nitrosomonas
Belong to.
The present invention makes full use of the bio-carrier of heterotrophism granule sludge to act on, and provides greatly for the growth of Autotrophic nitrification bacterium
Measure attachment point.Meanwhile this slow disadvantage of nitrobacteria multiplication rate is made up by adding external source aerobic ammonia oxidation bacterium, larger
Selection pressure realize the fast enriching of aerobic ammonia oxidation bacterium in heterotrophism granule sludge, the settling property for giving full play to AGS is good,
The advantages such as high resistance to poison provide technical support for waste water innoxious governance.Autotrophic nitrification granule sludge is tamed with other inoculations AGS
It compares, the method for the present invention can effectively reduce impact of the drastic change of service condition to inoculation AGS stability, in a short time realization
Intragranular heterotroph is by the replacement of aerobic ammonia oxidation bacterium.
Detailed description of the invention
Fig. 1 be in lab scale SBR aerobic ammonia oxidation granule sludge to the removal effect figure of ammonia nitrogen；
Fig. 2 is the domestication of aerobic ammonia oxidation granule sludge and flora composition in lab scale SBR；
Fig. 3 be in pilot scale SBR aerobic ammonia oxidation granule sludge to the removal effect figure of ammonia nitrogen；
Fig. 4 is the domestication of aerobic ammonia oxidation granule sludge and flora composition in pilot scale SBR.
Specific embodiment
A kind of fast culture process of aerobic ammonia oxidation granule sludge, is first inoculated with the aerobic particle mud based on heterotroph
Start SBR as carrier in sequencing batch reactor (SBR).The SBR is cylindrical reactor, and ratio of height to diameter is 4~20.It is described
The dominant bacteria of heterotroph in anaerobic ammonium oxidation granule sludge is Nitromonas.The present embodiment is inoculated in aerobic particle mud
Dominant bacteria is Zoogloea.After being seeded to reactor, initial reactor MLSS >=3000mg/L or more of reactor is apparent to rise
Gas velocity >=1.2cm/s, sedimentation time≤5 minute.For accelerate Autotrophic nitrification granule sludge formation, by gradually reducing into water
COD value until all substituted by inorganic carbon source to tame aerobic ammonia oxidation granule sludge, by the determination of mud granule rate into
Influent COD reduces 100mg/L daily when water COD fall, i.e. particle rate >=80%, otherwise maintains influent COD constant, instead
Polyphony control is replaced up to all inorganic carbon sources of influent COD, organic concentration zero.Water inlet is in domestication aerobic ammonia oxidation particle
Inorganic high ammonia-nitrogen wastewater is gradually converted by organic sewage in sludge process, which provides carbon by sodium bicarbonate
Source, between 60~320mg/L of influent ammonia nitrogen.Inlet flow-patterm is maintained between 7~9 during domestication, and TN (total nitrogen content) and TP are (total
Phosphorus content) it is constant.(every milliliter of concentrate of external source high-efficiency aerobic ammonia oxidizing bacteria is added into SBR while reducing influent COD
The active nitrobacteria quantity of bacterium solution is greater than 300,000,000), it is final fast to promote the fast enriching of aerobic ammonia oxidation bacterium in AGS
Speed turns out the aerobic ammonia oxidation granule sludge that can bear high ammonia nitrogen loading.High-efficiency aerobic ammonia oxidation bacteria concentrate dosage is 1
Milliliter bacterium solution/liter reactor mixed liquor, each period, adding the time continued to reactor with the disposable injection reactor of water inlet
Until water outlet nitrite nitrogen accumulation rate is more than 90%.
Aerobic ammonia oxidation granule sludge culture Success Flag are as follows: the SVI of granule sludge is maintained at 50mL/g hereinafter, granulating
Rate is maintained at 90% or more；Nitrite nitrogen accumulation rate and 90% or more is held in the removal rate of influent ammonia nitrogen.It hereafter can root
Different disposal target is realized according to needing to change influent ammonium concentration or nitrogen load.
Case 1
It is inoculated with AGS and starts lab scale SBR (effective height 180cm, internal diameter 10.5cm, ratio of height to diameter 17, dischargeable capacity
15.58L), apparently rise gas velocity between 1.2~1.5cm/s, rate of water exchange 50%, 6h is a period, and wherein the sedimentation time is 2
Minute.The seed sludge is the AGS of stable operation under practical septic tank wastewater, and color is in glassy yellow, and regular shape is mostly spherical shape
Or spheroid shape, starting MLSS are 4000mg/L, MLVSS/MLSS 0.56, SVI 40mg/L, EPS and PN/PS are respectively
25.1mg/g MLSS and 0.22.Inlet flow-patterm maintains between 7.5-8.7, and influent COD is down to zero by 600mg/L in 6 days, water inlet
Ammonia nitrogen is 140mg/L, and nitrogen load is 0.28kg N/m3·d.For nitrobacteria concentrate purchased from the net, main component is aerobic ammonia
Oxidizing bacteria, effective microbe content >=10*109CFU/mL.After 1~20 day continuously adds nitrobacteria concentrate, this is measured
NAR is maintained at 90% or more in post-reactor；It runs 31 days post-reactors and 90% is risen to the removal rate of ammonia nitrogen, see Fig. 1, it is good
The relative abundance of anaerobic ammonium oxidation bacterium reaches 27.87%, sees Fig. 2.
Case 2
It is inoculated with AGS and starts pilot scale SBR (effective height 180cm, internal diameter 29.2cm, ratio of height to diameter 6, dischargeable capacity
120.5L), apparently rise gas velocity in 1.25cm/s, rate of water exchange 60%, 6h is a period, and wherein the sedimentation time is 5 minutes.This connects
Kind sludge is the AGS of stable operation under practical septic tank wastewater, and color is in glassy yellow, and regular shape is mostly spherical or spheroid shape,
Starting MLSS is 4000mg/L, MLVSS/MLSS 0.56, and SVI 40mg/L, EPS and PN/PS are respectively 25.1mg/g MLSS
And 0.22.Inlet flow-patterm maintains between 6-9, and influent COD is down to zero by 600mg/L in 10 days, influent ammonia nitrogen 167mg/L, nitrogen
Load is 0.4kg N/m3·d.For nitrobacteria concentrate purchased from the net, main component is aerobic ammonia oxidation bacterium, effective micro- life
Object content >=10*106CFU/mL.Nitrobacteria concentrate, the NAR in 14 days post-reactors of survey are continuously added in 1~14 day
Increase to 90% or more；It runs 37 days post-reactors and 90% is risen to the removal rate of ammonia nitrogen, see Fig. 3, aerobic ammonia oxidation bacterium
Relative abundance reach 17.27%, see Fig. 4.
The present invention can effectively reduce impact of the drastic change of service condition to different oxygen granule sludge stability is inoculated with, at one month
Left and right can be achieved heterotroph in particle and substantially reduce Autotrophic nitrification granule sludge culture institute by the replacement of aerobic ammonia oxidation bacterium
The time needed.The aerobic ammonia oxidation granule sludge turned out can bear high ammonia nitrogen loading, and realize stable short distance nitration phenomenon,
Removal for ammonia nitrogen in China's mine wastewater provides a kind of new approaches.
Claims (8)
1. a kind of fast culture process of aerobic ammonia oxidation granule sludge, which is characterized in that inoculation is aerobic based on heterotroph
Granule sludge is used as carrier into sequencing batch reactor, then to be zero tame and docile by gradually reducing influent COD value to organic concentration
Change aerobic ammonia oxidation granule sludge, and adds High-efficient Nitrobacteria concentrate during domestication to promote autotrophic bacterium in particle dirt
Fast enriching in mud, fast culture go out aerobic ammonia oxidation granule sludge.
2. a kind of fast culture process of aerobic ammonia oxidation granule sludge as described in claim 1, which is characterized in that described to connect
Kind aerobic particle mud, autotrophic bacterium ratio≤10%, sinking speed >=15m/h are seeded to MLSS >=3000mg/ after reactor
L。
3. a kind of fast culture process of aerobic ammonia oxidation granule sludge as described in claim 1, which is characterized in that the sequence
Batch reactor is cylindrical reactor, and ratio of height to diameter is 4~20, and the reactor sedimentation time controlled within 5 minutes, apparent to rise
Gas velocity is in 1.2cm/s or more.
4. a kind of fast culture process of aerobic ammonia oxidation granule sludge as described in claim 1, which is characterized in that it is described into
Water is gradually converted into inorganic high ammonia-nitrogen wastewater by organic sewage during taming aerobic ammonia oxidation granule sludge, the inorganic high ammonia
Nitrogen waste water provides carbon source by sodium bicarbonate, between 60~320mg/L of influent ammonia nitrogen, pH 6-9.
5. a kind of fast culture process of aerobic ammonia oxidation granule sludge as described in claim 1, which is characterized in that the height
Effect nitrobacteria concentrate is commercially available cleaning aquarium nitrobacteria concentrate, and contained bacterium is aerobic ammonia oxidation bacterium,
The active nitrobacteria quantity of every milliliter of bacterium solution is greater than 300,000,000.
6. a kind of fast culture process of aerobic ammonia oxidation granule sludge as claimed in claim 1 or 5, which is characterized in that institute
Stating High-efficient Nitrobacteria concentrate minimum dosage is 1 milliliter of bacterium solution/liter reactor mixed liquor, and adding the time continues to reactor
Water outlet nitrite nitrogen accumulation rate be more than 90% until.
7. a kind of fast culture process of aerobic ammonia oxidation granule sludge as described in claim 1, which is characterized in that described good
Anaerobic ammonium oxidation granule sludge tames Success Flag are as follows: the SVI of granule sludge is maintained at 50mL/g hereinafter, particle rate is maintained at
90% or more, nitrite nitrogen accumulation rate and 90% or more is held in the removal rate of influent ammonia nitrogen.
8. a kind of fast culture process of aerobic ammonia oxidation granule sludge as described in claim 1, which is characterized in that described good
The dominant bacteria of heterotroph in anaerobic ammonium oxidation granule sludge is Nitromonas.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112479379A (en) * | 2020-12-11 | 2021-03-12 | 江西理工大学 | Ionic type rare earth mine high ammonia nitrogen wastewater aerobic granular sludge coupling denitrification method |
| CN114162970A (en) * | 2021-12-10 | 2022-03-11 | 上海问鼎环保科技有限公司 | Efficient denitrification method for aerobic granular sludge |
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