CN110243964A - In a kind of water treatment technology in microbial body guanosine tetraphosphate HPLC detection method - Google Patents

In a kind of water treatment technology in microbial body guanosine tetraphosphate HPLC detection method Download PDF

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CN110243964A
CN110243964A CN201910552292.2A CN201910552292A CN110243964A CN 110243964 A CN110243964 A CN 110243964A CN 201910552292 A CN201910552292 A CN 201910552292A CN 110243964 A CN110243964 A CN 110243964A
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guanosine tetraphosphate
concentration
guanosine
tetraphosphate
solution
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金鹏康
任童
金鑫
石烜
池玉蕾
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Xian University of Architecture and Technology
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/065—Preparation using different phases to separate parts of sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N2030/387—Turbulent flow of mobile phase

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Abstract

The present invention relates to sewage treatments and biochemical analysis and detection field, disclose a kind of HPLC detection method of guanosine tetraphosphate in microbial body in water treatment technology, specifically: in such a way that low temperature strong acid extracts microbial body inner nucleotide, effectively extract the intracorporal ucleotides substance of microorganism, then by selecting biphosphate potassium concn for 5~10mM, the ultra-pure water solution that pH value is 3.9~4.1 is as mobile phase A, biphosphate potassium concn is 0.5M, sodium sulfate concentration is 0.5M, the ultra-pure water solution that pH value is 5.3~5.5 is as Mobile phase B, it is detected and is calculated using high performance liquid chromatography combined standard curve method, realize the Accurate Determining to guanosine tetraphosphate content in water treatment micro-organism, base is established to improve microorganism growth conditions adjustment mechanism research in water treatment technology Plinth.

Description

In a kind of water treatment technology in microbial body guanosine tetraphosphate HPLC detection method
Technical field
The present invention relates to sewage treatments and biochemical analysis and detection field, specifically, being related in a kind of water treatment technology The HPLC detection method of guanosine tetraphosphate in microbial body.
Background technique
Guanosine tetraphosphate is called ppGpp, molecular formula C10H17N5O17P4, molecular weight 603.2, structure is as follows:
Guanosine tetraphosphate is a kind of guanosine acid molecule of hyperphosphorylation, is microorganism reply varying environment stress pressure Power carries out the alarm signal molecule of physiological adaptation gene expression regulation, with effect is extensive, timeliness is significant, micro easy decomposition The characteristics of.
The horizontal variation of guanosine tetraphosphate can lead to as DNA replication dna is obstructed, rRNA synthesis inhibits and drop in microbial cell Solution, the differentially expressed and metabolic enzyme of gene a series of important life processes such as activate or inhibit and change.Sewage Biological Treatment It is one of widest method in Modern sewage processing application, mainly by the decomposition of microorganism the organic matter in sewage It is converted into simple inorganic matter, makes to purify the sewage.However, the growing environment condition of microorganism is more harsh, nutriment, Temperature, pH and dissolved oxygen etc. can all influence the normal growth breeding of microorganism, to influence the effect of sewage treatment.Cause This can be microorganism in biological wastewater treatment process by the analyzing detecting method of guanosine tetraphosphate in microorganisms body Growth conditions be monitored, as sewage treatment process operation conditions monitoring provide new thinking.
The method for being usually used in guanylic acid analysis of molecules at present mainly has thin layer chromatography (TLC), ion-exchange chromatography (IEC), high performance liquid chromatography (HPLC), gel electrophoresis (PAGE), high performance capillary electrophoresis (HPCE) etc.；Commonly mention Method is taken to have strong acid extraction, organic solvent extraction, thermal extraction, ultrasonic wave extraction etc..Due to the micro easy decomposition of ucleotides substance Property, detection must assure that stringent environmental condition and quick analysis time, brings error for actual analysis in this way And inconvenience.Stefanie L.Vogt et al. is by HPLC- UV detection device to four phosphorus of guanosine in pseudomonas aeruginosa Acid carry out it is qualitative and quantitative, although this method process is simple, sample appearance time is long, Linear Quasi it is right it is poor, separating degree is lower, To qualitative and quantitatively bring interference.It is therefore desirable to improve original method, explores and develop the quantitative inspection of new fast qualitative Survey method, it is horizontal for detecting the intracorporal guanosine tetraphosphate of microorganism in water treatment technology.
Summary of the invention
In order to solve the problems in the existing technology, the object of the present invention is to provide microorganisms in a kind of water treatment technology The HPLC detection method of internal guanosine tetraphosphate.
In order to achieve the object of the present invention, the present invention has passed through in a kind of water treatment technology guanosine tetraphosphate in microbial body HPLC detection method, which comprises
(1) guanosine tetraphosphate standard items are dissolved in water, are configured to the guanosine tetraphosphate standard solution of various concentration；
(2) the guanosine tetraphosphate standard solution of various concentration is analyzed respectively using high performance liquid chromatography, chromatography Analysis condition is as follows:
Mobile phase A is the ultra-pure water solution that biphosphate potassium concn is 5~10mM, pH value is 3.9~4.1；Such as it flows Phase A is the ultra-pure water solution that biphosphate potassium concn is 7mM, pH value is 4.0；
Mobile phase B is the ultrapure water that biphosphate potassium concn is 0.5M, sodium sulfate concentration 0.5M, pH value are 5.3~5.5 Solution；
Sample introduction simultaneously carries out gradient elution, and eluent gradient A:B is by 100:0 to 0:100, flow velocity 0.8mL/min；It is equivalent to Mobile phase A gradually decreases down 0 from 100% within 0~20min time, and Mobile phase B gradually rises up to 100% from 0；
Using the concentration of guanosine tetraphosphate standard solution as abscissa, using the chromatography main peak area measured as ordinate, draw The standard curve that chromatography main peak area processed changes with guanosine tetraphosphate concentration, obtains linear equation；
(3) using formic acid cracking tested microorganism (such as Microbial Communities in Activated Sludge), it is intracorporal to extract tested microorganism Guanosine tetraphosphate obtains testing sample solution, is analyzed using high performance liquid chromatography the testing sample solution, chromatography point Analysis condition is identical as step (2)；
(4) the chromatography main peak area of the testing sample solution obtained according to step (3) analysis, it is true to substitute into step (2) The linear equation of fixed standard curve, can be calculated the guanosine tetraphosphate concentration of testing sample solution, and then treat micrometer The intracorporal guanosine tetraphosphate of biology carries out qualitative and quantitative.
Further, in step (3), tested microorganism is added in 90% ice-cold (v/v) formic acid and is mixed, cracked on ice After 2h, centrifuging and taking supernatant, obtains testing sample solution under the conditions of 4 DEG C, 12000rpm.This step is extracted using low temperature strong acid The mode of microbial body inner nucleotide can effectively extract the intracorporal ucleotides substance of microorganism.
Further, the activated sludge that the tested microorganism is generated from water process, the tested microorganism obtain Take method are as follows:
The activated sludge is centrifuged under the conditions of 4 DEG C, 12000rpm, thallus is collected and (supernatant is toppled over, after retaining centrifugation Sediment)；(routine operation, it is intended to remove under activated sludge system is cleaned to the thallus being collected into using PBS buffer solution The substances such as exotic organic material, the mode being centrifuged after being cleaned multiple times can be used and carry out), obtain tested microorganism.
Preferably, the concentration range of the guanosine tetraphosphate standard solution of prepared various concentration exists in step (1) Between 5.03-25.13 μ g/mL.
More preferably, in step (1), compound concentration be respectively 5.03 μ g/mL, 10.05 μ g/mL, 15.08 μ g/mL, The guanosine tetraphosphate standard solution of 20.11 μ g/mL, 25.13 μ g/mL.
Specifically, in step (2), chromatographic column are as follows: Agela Technologies Venusil SAX 4.6 × 250mm, 5 μm.Filler is high pure spherical silica gel, and Bonded Phase is quaternary amine base anionic group.At 25~30 DEG C, Detection wavelength is for column temperature control 260nm。
The percentage composition that the present invention is mentioned to unless otherwise specified, refers both to volumn concentration；The raw material being related to or examination Agent is ordinary commercial products, and the operation being related to is this field routine operation unless otherwise specified.
On the basis of common knowledge of the art, above-mentioned each optimum condition, can be combined with each other, obtain specific embodiment party Formula.
The beneficial effects of the present invention are:
The present invention provides the HPLC detection method of guanosine tetraphosphate in microbial body in a kind of water treatment technology, use is low Warm strong acid extracts the mode of microbial body inner nucleotide, effectively extracts the intracorporal ucleotides substance of microorganism, then passes through choosing Suitable mobile phase is selected, is detected and is calculated using high performance liquid chromatography combined standard curve method, is realized to the micro- life of water process The Accurate Determining of guanosine tetraphosphate content in object is established to improve microorganism growth conditions adjustment mechanism research in water treatment technology Basis.
The accurate qualitative and quantitative of guanosine tetraphosphate in microbial body under Sewage Biological Treatment system can be achieved in the present invention, and Detectable substance matter retention time is short, and separating degree is high, and detection efficiency is high, and easy to operate cost is relatively low.It is examined using the method for the present invention It surveys, guanosine tetraphosphate component linear relationship is preferable, degree of fitting is high.
Detailed description of the invention
Fig. 1 is the HPLC chromatogram of various concentration guanosine tetraphosphate standard solution in embodiment 1.
Fig. 2 is the canonical plotting of guanosine tetraphosphate in embodiment 1.
Fig. 3 is the HPLC chromatogram that ppGpp in microbial body in activated sludge system is detected in embodiment 1.
Fig. 4 is the HPLC chromatogram of ppGpp standard items sample introduction twice in comparative example 2.
Specific embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It will be appreciated that following real Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this field Art personnel without departing from the spirit and purpose of the present invention, can carry out various modifications and replace to the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1
1, instrument and reagent
Shimadzu LC-2010AHT high performance liquid chromatograph, Shimadzu RF-20A fluorescence detector (Japanese Shimadzu Corporation)；AND essence Close electronic balance AR4504 (Japanese AND Ai Ande company)；Thermo Sorvall RC-6Pius lands centrifuge (U.S.'s match Mo Feishier company)；MILLIPORE ultrapure water machine (MILLIPORE company, the U.S.)
Activated sludge is derived from the 5th sewage treatment plant's returned sludge of Xi'an.
Standard items: guanosine tetraphosphate (ppGpp) standard items N-6001, molecular formula C10H17N5O17P4, molecular weight 603.2,1 μm ol pure material is dissolved in 100mM water, is purchased from U.S. TriLinK Biotechnologies company.
Chromatographic column: Agela Technologies Venusil SAX 4.6 × 250mm, 5 μm, Serial No.: 019171。
Guaranteed reagent: potassium dihydrogen phosphate, sodium sulphate, potassium hydroxide, phosphoric acid, formic acid, disodium hydrogen phosphate.
Resistivity is the ultrapure water of 18.2 Ω.
2, method
The preparation of 2.1 mobile phases
Mobile phase A: it weighs 0.95g potassium dihydrogen phosphate and is dissolved in 500mL ultrapure water, after being completely dissolved extremely with volumetric flask constant volume 1000mL to get arrive 7mM potassium dihydrogen phosphate.By phosphorus acid for adjusting pH to 4.0, with 0.22 μm of filtering with microporous membrane and surpass Sound degassing 15min.
Mobile phase B: weighing 68.045g potassium dihydrogen phosphate and 71.03g sodium sulphate is dissolved in 500mL ultrapure water, is completely dissolved Afterwards with volumetric flask constant volume to 1000mL to get arrive 0.5M potassium dihydrogen phosphate+0.5M metabisulfite solution.Pass through potassium hydroxide solution tune PH to 5.4 is saved, with 0.22 μm of filtering with microporous membrane and ultrasonic degassing 15min.
The preparation of 2.2 standard solution
Guanosine tetraphosphate standard items N-6001 is dissolved in the ice-cold ultrapure water of 24mL to get being 25.13 μ g/mL to concentration Standard items mother liquor, precision measurement standard mother liquor 1mL, 2mL, 3mL, 4mL is to 5mL volumetric flask cold ultrapure water constant volume on the rocks respectively To scale, shake up.Obtain the hybrid standard liquid and 25.13 of 5.03 μ g/mL, 10.05 μ g/mL, 15.08 μ g/mL, 20.11 μ g/mL μ g/mL standard mother liquor.
The preparation of 2.3 sample solutions
Activated sludge is collected in the 50mL centrifuge tube weighed, 4 DEG C, 12000rpm is centrifuged 5min, collects thallus simultaneously Weigh thallus weight in wet base；The PBS buffer solution for being 7.38 using potassium dihydrogen phosphate and disodium hydrogen phosphate configuration pH；The wet bacterium of about 1g is taken, Every wet bacterium of 0.1g adds 400 μ L ice cold PBS buffers；It repeats above-mentioned centrifugation weighing operation and adds PBS buffer solution；Then 4 DEG C, 12000rpm is centrifuged 5min, after collecting thallus and weighing, according to 90% formic acid that the wet bacterium of every 0.2g adds 1mL ice-cold, mixes on ice Crack 2h；4 DEG C, 12000rpm centrifugation 15min takes supernatant to be stored in -40 DEG C of refrigerators.
2.4 chromatographicconditions and result
Using Agela 4.6 × 250mm of Technologies Venusil SAX, 5 μm of chromatographic columns, 20 μ L of sample volume, inspection Survey wavelength is 260nm；Flow velocity is 0.8mL/min；27 DEG C of column temperature, mobile phase: 7mM potassium dihydrogen phosphate (A), 0.5M di(2-ethylhexyl)phosphate Hydrogen potassium+0.5M metabisulfite solution (B) is mobile phase.
The flowing phase concentration of 1 HPLC of table detection ppGpp gradient elution
High performance liquid chromatograph is adjusted, chromatographic column is balanced, using liquid chromatograph to the guanosine tetraphosphate standard solution Liquid-phase chromatographic analysis is carried out, the peak area recorded and analyzed, twice, chromatogram is such as the standard solution difference sample introduction of each concentration Shown in Fig. 1, the peak of retention time to be at 13min be guanosine tetraphosphate in figure, remaining is impurity peaks, and peak area is as shown in table 2 below, And guanosine tetraphosphate content-peak area standard curve (Fig. 2) is drawn using external standard method, obtain standard curve regression equation, y= 24582x-2304, R2=0.9994.
2 concentration of standard solution μ g/mL of table and peak area data loger
Test solution is used and carries out liquid-phase chromatographic analysis with the identical chromatographic condition of standard solution, and is remembered simultaneously Peak area is recorded, chromatogram is as shown in figure 3, obtain Unit Weight bacterium in the sludge sample according to standard curve regression equation calculation The guanosine tetraphosphate concentration of body.
Embodiment 2
For the feasibility and accuracy for verifying above-described embodiment 1, precision test is carried out, test uses concentration for 15.08 μ G/mL prepare guanosine tetraphosphate standard solution, continuous sample introduction 8 times, using with the identical chromatographic condition of embodiment 1, measurement The results are shown in Table 3.
Table 3
By table 3 it can be concluded that the RSD of peak area is less than 2%, therefore stability of instrument is good.
Comparative example 1
This comparative example the difference from embodiment 1 is that, 90% formic acid is replaced using ice-cold 5% perchloric acid and extracts nucleosides Acid adjusts pH with sodium hydroxide as 6~6.5, remaining operation is same as Example 1.
Sample ppGpp concentration records table under 4 Different Extraction Method of table
4 kinds of different types of activated sludge samples are selected respectively, take perchloric acid to extract dense with formic acid extraction progress ppGpp Degree detection, the results are shown in Table 4, it can be found that the relative standard deviation of two kinds of extracting mode acquired results is respectively less than 2%, but first The relative standard deviation of sour extracting method result is generally less than perchloric acid extracting method.And it is measured in formic acid extracting method PpGpp concentration is above perchloric acid extracting method, i.e., the method for extracting active sludge microorganism nucleic acid in vivo using formic acid is more excellent It is extracted in using perchloric acid.
Comparative example 2
This comparative example the difference from embodiment 1 is that, using ultrapure water as Mobile phase B, acetonitrile (chromatographic grade) is as stream Dynamic phase A carries out efficient liquid phase chromatographic analysis, remaining operation is same as Example 1.
Efficient liquid phase chromatographic analysis is carried out to standard solution, the standard items difference sample introduction of each concentration twice, records and analyzes Obtained peak area.Fig. 4 is the liquid chromatogram of the ppGpp standard items sample introduction of 15.08 μ g/mL twice, retention time in figure It is the chromatographic peak of guanosine tetraphosphate at 37min, remaining is impurity peaks.The peak area of each concentration is as shown in table 5, and obtains Standard curve regression equation Y=30500X-18744, R2=0.9962.
5 concentration of standard solution of table and peak area data loger
By comparison diagram 4 and Fig. 1 it is found that using ultrapure water as mobile phase A, when acetonitrile is as Mobile phase B, in 20min Interior Mobile phase B rises to 100%, ppGpp standard items there is no appearance from 0, continues 100% Mobile phase B until 45min, ppGpp The retention time of standard items is 37.6min.Therefore the present invention reduces 20 in the retention time of detectable substance relative to this comparative example More minutes, and the correlation of standard curve is better than comparative example.
By observing Fig. 4 it is found that when organic phase is as mobile phase, a lesser miscellaneous peak is had before standard items appearance Miscellaneous peak area tends to become larger when occurring, and can be seen that second of sample introduction by repeated experiment twice, and standard items peak area becomes In becoming smaller.It is related with the property of guanosine tetraphosphate and retention time to analyze this phenomenon, guanosine tetraphosphate is easy to decompose at normal temperature, The retention time of comparative example is longer, analyzes the catabolite that this miscellaneous peak is guanosine tetraphosphate.Therefore the flowing that this field routinely uses Mutually it is not suitable for carrying out the HPLC detection of guanosine tetraphosphate.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.

Claims (8)

1. in a kind of water treatment technology in microbial body guanosine tetraphosphate HPLC detection method, which is characterized in that the method Include:
(1) guanosine tetraphosphate standard items are dissolved in water, are configured to the guanosine tetraphosphate standard solution of various concentration；
(2) the guanosine tetraphosphate standard solution of various concentration is analyzed respectively using high performance liquid chromatography, chromatography Condition is as follows:
Mobile phase A is the ultra-pure water solution that biphosphate potassium concn is 5~10mM, pH value is 3.9~4.1；
Mobile phase B be biphosphate potassium concn be 0.5M, sodium sulfate concentration 0.5M, pH value be 5.3~5.5 it is ultrapure water-soluble Liquid；
Sample introduction simultaneously carries out gradient elution, and eluent gradient A:B is by 100:0 to 0:100, flow velocity 0.8mL/min；
Using the concentration of guanosine tetraphosphate standard solution as abscissa, using the chromatography main peak area measured as ordinate, color rendering The standard curve that spectrum main peak area changes with guanosine tetraphosphate concentration, obtains linear equation；
(3) tested microorganism is cracked using formic acid, extracts the intracorporal guanosine tetraphosphate of tested microorganism, it is molten obtains sample to be tested Liquid analyzes the testing sample solution using high performance liquid chromatography, and chromatographiccondition is identical as step (2)；
(4) the chromatography main peak area of the testing sample solution obtained according to step (3) analysis, what substitution step (2) determined The guanosine tetraphosphate concentration of testing sample solution can be calculated in the linear equation of standard curve, and then to tested microorganism Intracorporal guanosine tetraphosphate carries out qualitative and quantitative.
2. the method according to claim 1, wherein ice-cold 90% is added in tested microorganism in step (3) (v/v) it is mixed in formic acid, after cracking on ice, centrifuging and taking supernatant obtains testing sample solution.
3. according to the method described in claim 2, it is characterized in that, the tested microorganism is dirty from the activity that water process generates Mud, the acquisition methods of the tested microorganism are as follows:
The activated sludge is centrifuged under the conditions of 4 DEG C, 12000rpm, collects thallus；Using PBS buffer solution to the bacterium being collected into Body is cleaned, and tested microorganism is obtained.
4. described in any item methods according to claim 1~3, which is characterized in that in step (1), prepared various concentration Guanosine tetraphosphate standard solution concentration range between 5.03-25.13 μ g/mL.
5. according to the method described in claim 4, it is characterized in that, in step (1), compound concentration be respectively 5.03 μ g/mL, The guanosine tetraphosphate standard solution of 10.05 μ g/mL, 15.08 μ g/mL, 20.11 μ g/mL, 25.13 μ g/mL.
6. according to the method described in claim 5, it is characterized in that, in step (2), chromatographic column are as follows: Agela Technologies Venusil SAX 4.6 × 250mm, 5 μm.
7. according to the method described in claim 6, it is characterized in that, column temperature is controlled at 25~30 DEG C in step (2).
8. the method according to the description of claim 7 is characterized in that in step (2), Detection wavelength 260nm.
CN201910552292.2A 2019-06-25 2019-06-25 In a kind of water treatment technology in microbial body guanosine tetraphosphate HPLC detection method Pending CN110243964A (en)

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