CN110243900B - 一种聚三聚氰胺修饰电极的应用 - Google Patents
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- 229920000877 Melamine resin Polymers 0.000 title claims abstract description 27
- JDSHMPZPIAZGSV-UHFFFAOYSA-N melamine Chemical compound NC1=NC(N)=NC(N)=N1 JDSHMPZPIAZGSV-UHFFFAOYSA-N 0.000 title claims abstract description 27
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims abstract description 23
- 235000005979 Citrus limon Nutrition 0.000 claims abstract description 14
- 244000248349 Citrus limon Species 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 5
- UJMBCXLDXJUMFB-GLCFPVLVSA-K tartrazine Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)C1=NN(C=2C=CC(=CC=2)S([O-])(=O)=O)C(=O)C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 UJMBCXLDXJUMFB-GLCFPVLVSA-K 0.000 claims description 8
- 229960000943 tartrazine Drugs 0.000 claims description 8
- 235000012756 tartrazine Nutrition 0.000 claims description 8
- 239000004149 tartrazine Substances 0.000 claims description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 6
- 239000008055 phosphate buffer solution Substances 0.000 claims description 5
- 238000002484 cyclic voltammetry Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 4
- 238000001903 differential pulse voltammetry Methods 0.000 claims description 3
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 claims description 3
- 239000003792 electrolyte Substances 0.000 claims description 3
- 229910052697 platinum Inorganic materials 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000004044 response Effects 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 description 5
- 229920001940 conductive polymer Polymers 0.000 description 4
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- -1 nitrite ions Chemical class 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001318 differential pulse voltammogram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000576 food coloring agent Substances 0.000 description 1
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- 230000008520 organization Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920000580 poly(melamine) Polymers 0.000 description 1
- 229920001197 polyacetylene Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
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Abstract
本发明涉及一种聚三聚氰胺修饰电极的应用。包括以下步骤：聚三聚氰胺修饰电极的制备、聚三聚氰胺修饰电极对亚硝酸盐和柠檬黄的同时检测。本发明的有益效果是：聚三聚氰胺修饰电极对亚硝酸盐和柠檬黄均具有良好的电流响应。
Description
技术领域
本发明涉及一种聚三聚氰胺修饰电极的应用，属于电分析化学和食品化学的研究领域。
技术背景
由于亚硝酸盐能防止细菌生长，所以在食品中它常被用作防腐剂。世界卫生组织和欧盟食品科学委员会将亚硝酸盐每人每天摄入的最大量为0.07毫克亚硝酸根离子/千克，过量的亚硝酸盐可与二价铁血红蛋白结合形成三价铁血红蛋白，导致一种被称为“蓝色婴儿综合征”的病症。因此，对于食品中亚硝酸盐的测定是必要的。
柠檬黄是一种橙色单偶氮吡唑啉酮染料，也是一种被广泛使用的食品着色剂，常常存在于饮料和糖果等食品中。过量的柠檬黄不仅可引起儿童过敏和多动症等多个不良反应，而且对人体中的肝脏和肾脏有严重的影响。因此，对柠檬黄的检测也是非常重要的。
导电聚合物又称导电高分子,是指通过掺杂等手段制备的电导率在导体和半导体范围内的聚合物。自20世纪70年代末发现聚乙炔导电聚合物以来，由于其独特的电、热和机械性能而引起了相当大的关注。聚三聚氰胺是一种具有许多氨基基团的导电聚合物，可通过三聚氰胺中的聚合而得到。目前已有了关于聚合物分别测定亚硝酸盐和柠檬黄的报道，但关于聚三聚氰胺同时检测亚硝酸盐和柠檬黄的研究尚未有报道。
发明内容
本发明的目的在于提供一种可同时测定亚硝酸盐和柠檬黄的聚三聚氰胺修饰电极。
本发明所述一种聚三聚氰胺修饰电极的应用，包括以下步骤：
a、聚三聚氰胺修饰电极的制备：
采用三电极体系，通过循环伏安法制备聚三聚氰胺修饰电极，所制备的聚三聚氰胺修饰电极在使用之前都用超纯水冲洗干净；
b、聚三聚氰胺修饰电极对亚硝酸盐和柠檬黄的同时检测：
在电化学工作站上对亚硝酸盐和柠檬黄同时进行检测，检测采用三电极体系，聚三聚氰胺修饰电极为工作电极，铂片电极为对电极，饱和甘汞电极为参比电极，电解液为一定pH值的0.1M磷酸盐缓冲溶液，在以上条件下用差分脉冲伏安法对亚硝酸盐和柠檬黄进行同时测定。
进一步地，步骤a中循环伏安法扫描圈数是15圈。
进一步地，步骤b中亚硝酸盐的浓度为1～2mM；柠檬黄的浓度为1～2mM；0.1M磷酸盐缓冲溶液的pH值为2.0～5.0。
本发明的有益效果是：聚三聚氰胺修饰电极对亚硝酸盐和柠檬黄均具有良好的电流响应。
附图说明
下面结合附图对本实验进一步说明。
图1为实施例一中聚三聚氰胺修饰电极在0.1M磷酸盐缓冲溶液中同时测定亚硝酸盐和柠檬黄的差分脉冲伏安图。
具体实施方式
现在结合具体实施例对本发明做进一步说明，以下实施例旨在说明本发明而不是对本发明的进一步限定。
实施例一：
本发明对亚硝酸盐和柠檬黄同时进行检测，采用三电极体系，聚三聚氰胺修饰电极为工作电极，铂片电极为对电极，饱和甘汞电极为参比电极，电解液为pH4.0的0.1M磷酸盐缓冲溶液，在以上条件下用差分脉冲伏安法对亚硝酸盐和柠檬黄同时进行检测，从图1中可以看出，同时出现了亚硝酸盐和柠檬黄的氧化峰。可以得出，该聚三聚氰胺修饰电极对亚硝酸盐和柠檬黄同时进行检测是有效的。
Claims (2)
1.一种聚三聚氰胺修饰电极的应用，步骤如下：
a、聚三聚氰胺修饰电极的制备：采用三电极体系，通过循环伏安法制备聚三聚氰胺修饰电极，所制备的聚三聚氰胺修饰电极在使用之前用超纯水冲洗干净；
b、聚三聚氰胺修饰电极对亚硝酸盐和柠檬黄的同时检测：在电化学工作站上对亚硝酸盐和柠檬黄进行同时检测，检测采用三电极体系，聚三聚氰胺修饰电极为工作电极，铂片电极为对电极，饱和甘汞电极为参比电极，电解液为pH4.0的0.1M磷酸盐缓冲溶液，用差分脉冲伏安法对亚硝酸盐和柠檬黄进行同时测定；其中，所述亚硝酸盐的浓度为1～2mM，柠檬黄的浓度为1～2mM。
2.根据权利要求1所述一种聚三聚氰胺修饰电极的应用，其特征是：所述步骤a中循环伏安法扫描圈数是15圈。
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102262115A (zh) * | 2011-07-28 | 2011-11-30 | 南京师范大学 | 测定三聚氰胺含量的电化学免疫传感器及制备方法和应用 |
| CN103487492A (zh) * | 2013-09-17 | 2014-01-01 | 常州大学 | 一种检测痕量双酚a的电化学传感器及其制备方法与应用 |
| CN106290512A (zh) * | 2016-08-31 | 2017-01-04 | 西北师范大学 | 修饰电极及其制备方法和应用 |
| CN109112570A (zh) * | 2018-08-03 | 2019-01-01 | 闽南师范大学 | 一种适用于高效电催化的聚氰胺复合物电极及其制备方法 |
| CN109358102A (zh) * | 2018-12-06 | 2019-02-19 | 湖南科技大学 | 一种快捷制备聚三聚氰胺导电聚合物电极的方法及其应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2232249A4 (en) * | 2007-12-13 | 2014-04-16 | Univ Monash | BIOCAPTOR SYSTEM ELECTROCHEMICAL NANOCOMPOSITE |
| US20150276647A1 (en) * | 2014-04-01 | 2015-10-01 | King Fahd University Of Petroleum And Minerals | Amperometric nitrate sensor |
| CN208505904U (zh) * | 2018-06-29 | 2019-02-15 | 天津果实科技有限公司 | 尿液十四项多排排列干化学分析试纸 |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102262115A (zh) * | 2011-07-28 | 2011-11-30 | 南京师范大学 | 测定三聚氰胺含量的电化学免疫传感器及制备方法和应用 |
| CN103487492A (zh) * | 2013-09-17 | 2014-01-01 | 常州大学 | 一种检测痕量双酚a的电化学传感器及其制备方法与应用 |
| CN106290512A (zh) * | 2016-08-31 | 2017-01-04 | 西北师范大学 | 修饰电极及其制备方法和应用 |
| CN109112570A (zh) * | 2018-08-03 | 2019-01-01 | 闽南师范大学 | 一种适用于高效电催化的聚氰胺复合物电极及其制备方法 |
| CN109358102A (zh) * | 2018-12-06 | 2019-02-19 | 湖南科技大学 | 一种快捷制备聚三聚氰胺导电聚合物电极的方法及其应用 |
Non-Patent Citations (3)
| Title |
|---|
| "Electropolymerized melamine for simultaneous determination of nitrite and tartrazine";Shanshan Wu 等;《Food Chemistry》;20200709;127532(1-7页) * |
| "示差脉冲极谱法测定柠檬黄";李翱楠 等;《广州化工》;20111231;第39卷(第21期);摘要，112页的1.1-2.1，114页的2.6 * |
| "纳米金/三聚氰胺修饰电极检测亚硝酸盐";张英 等;《分析试验室》;20130630;第32卷(第6期);摘要，63-64页的1.1-1.2，65页的2.2-2.3，图2 * |
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