CN110243804A - 一种新型拉曼探针及其制备方法 - Google Patents
一种新型拉曼探针及其制备方法 Download PDFInfo
- Publication number
- CN110243804A CN110243804A CN201910640946.7A CN201910640946A CN110243804A CN 110243804 A CN110243804 A CN 110243804A CN 201910640946 A CN201910640946 A CN 201910640946A CN 110243804 A CN110243804 A CN 110243804A
- Authority
- CN
- China
- Prior art keywords
- cell
- mcf
- raman microprobe
- raman
- pbs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001069 Raman spectroscopy Methods 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 210000004027 cell Anatomy 0.000 claims abstract description 63
- 238000001514 detection method Methods 0.000 claims abstract description 30
- 108091023037 Aptamer Proteins 0.000 claims abstract description 11
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 claims abstract description 11
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 claims abstract description 11
- 230000002093 peripheral effect Effects 0.000 claims abstract description 10
- 230000009881 electrostatic interaction Effects 0.000 claims abstract description 6
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 5
- 239000010410 layer Substances 0.000 claims description 21
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 15
- 239000010931 gold Substances 0.000 claims description 15
- 229910052737 gold Inorganic materials 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 15
- 238000005406 washing Methods 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 9
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 7
- SLAMLWHELXOEJZ-UHFFFAOYSA-N 2-nitrobenzoic acid Chemical compound OC(=O)C1=CC=CC=C1[N+]([O-])=O SLAMLWHELXOEJZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 210000002381 plasma Anatomy 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 230000001413 cellular effect Effects 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- 238000002955 isolation Methods 0.000 claims description 5
- 210000004698 lymphocyte Anatomy 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 239000007984 Tris EDTA buffer Substances 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 3
- 150000002343 gold Chemical class 0.000 claims description 3
- 239000000376 reactant Substances 0.000 claims description 3
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000004820 blood count Methods 0.000 claims description 2
- 210000003743 erythrocyte Anatomy 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 239000002356 single layer Substances 0.000 claims description 2
- 238000003786 synthesis reaction Methods 0.000 claims description 2
- 235000013339 cereals Nutrition 0.000 claims 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 23
- 210000005266 circulating tumour cell Anatomy 0.000 abstract description 10
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 abstract description 9
- 230000002255 enzymatic effect Effects 0.000 abstract description 6
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 230000003321 amplification Effects 0.000 abstract description 4
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 4
- 206010006187 Breast cancer Diseases 0.000 abstract description 2
- 208000026310 Breast neoplasm Diseases 0.000 abstract description 2
- 239000002105 nanoparticle Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 5
- 201000011510 cancer Diseases 0.000 description 4
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 description 3
- 230000002380 cytological effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229940044683 chemotherapy drug Drugs 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000032798 delamination Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000001795 light effect Effects 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002114 nanocomposite Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910640946.7A CN110243804B (zh) | 2019-07-16 | 2019-07-16 | 一种新型拉曼探针及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910640946.7A CN110243804B (zh) | 2019-07-16 | 2019-07-16 | 一种新型拉曼探针及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110243804A true CN110243804A (zh) | 2019-09-17 |
CN110243804B CN110243804B (zh) | 2021-12-28 |
Family
ID=67892441
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910640946.7A Active CN110243804B (zh) | 2019-07-16 | 2019-07-16 | 一种新型拉曼探针及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110243804B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113384694A (zh) * | 2021-04-25 | 2021-09-14 | 青岛科技大学 | 一种用于肿瘤诊疗一体化的纳米复合药物及其制备方法 |
CN113406053A (zh) * | 2021-06-22 | 2021-09-17 | 徐州医科大学 | 一种肿瘤细胞标志物miRNA-21的检测方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101434984A (zh) * | 2007-11-13 | 2009-05-20 | 中国科学院上海应用物理研究所 | 一种检测DNA的i-motif构象的光学方法 |
US20150240297A1 (en) * | 2012-07-31 | 2015-08-27 | Juan Carlos Jaime | Method for obtaining and detecting a marker of objects to be identified, related marker, authentication method and verification method |
CN109060756A (zh) * | 2018-06-01 | 2018-12-21 | 宁波美晶医疗技术有限公司 | 一种基于表面增强效应的血液中稀有细胞检测方法 |
CN109632765A (zh) * | 2019-01-28 | 2019-04-16 | 上海理工大学 | 一种外泌体表面蛋白检测方法 |
-
2019
- 2019-07-16 CN CN201910640946.7A patent/CN110243804B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101434984A (zh) * | 2007-11-13 | 2009-05-20 | 中国科学院上海应用物理研究所 | 一种检测DNA的i-motif构象的光学方法 |
US20150240297A1 (en) * | 2012-07-31 | 2015-08-27 | Juan Carlos Jaime | Method for obtaining and detecting a marker of objects to be identified, related marker, authentication method and verification method |
CN109060756A (zh) * | 2018-06-01 | 2018-12-21 | 宁波美晶医疗技术有限公司 | 一种基于表面增强效应的血液中稀有细胞检测方法 |
CN109632765A (zh) * | 2019-01-28 | 2019-04-16 | 上海理工大学 | 一种外泌体表面蛋白检测方法 |
Non-Patent Citations (3)
Title |
---|
JAEJUNG SONG ETAL.,: "Electrostatic interaction driven gold nanoparticle assembly on three-dimensional triangular pyramid DNA nanostructures", 《NEW JOURNAL OF CHEMISTRY》 * |
MANJARI BHAMIDIPATI ETAL.,: "SERS-Based Quantification of Biomarker Expression at the Single Cell Level Enabled by Gold Nanostars and Truncated Aptamers", 《BIOCONJUGATE CHEMISTRY》 * |
XIAOXIA WU ETAL.,: "Improved SERS Nanoparticles for Direct Detection of Circulating Tumor Cells in the Blood", 《ACS APPLIED MATERIALS & INTERFACES 》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113384694A (zh) * | 2021-04-25 | 2021-09-14 | 青岛科技大学 | 一种用于肿瘤诊疗一体化的纳米复合药物及其制备方法 |
CN113406053A (zh) * | 2021-06-22 | 2021-09-17 | 徐州医科大学 | 一种肿瘤细胞标志物miRNA-21的检测方法 |
Also Published As
Publication number | Publication date |
---|---|
CN110243804B (zh) | 2021-12-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Hartjes et al. | Extracellular vesicle quantification and characterization: common methods and emerging approaches | |
Zhang et al. | Rapid capture and nondestructive release of extracellular vesicles using aptamer-based magnetic isolation | |
Lewis et al. | Integrated analysis of exosomal protein biomarkers on alternating current electrokinetic chips enables rapid detection of pancreatic cancer in patient blood | |
Huerta-Nuñez et al. | A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy | |
Petchakup et al. | Advances in single cell impedance cytometry for biomedical applications | |
US9494557B2 (en) | Micro-fluidic system using micro-apertures for high throughput detection of cells | |
Russo et al. | The role of dielectrophoresis for cancer diagnosis and prognosis | |
Mousavi et al. | Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance | |
US20230015302A1 (en) | Methods for identifying viral infections and for analyzing exosomes in liquid samples by raman spectroscopy | |
CN104977277B (zh) | 一种可同时检测细胞内野生型和变异p53蛋白的纳米囊泡 | |
Irmer et al. | Extracellular vesicles in liquid biopsies as biomarkers for solid tumors | |
Jeong et al. | Analysis of extracellular vesicles using coffee ring | |
CN110243804A (zh) | 一种新型拉曼探针及其制备方法 | |
Wu et al. | A portable magnetic particle spectrometer for future rapid and wash-free bioassays | |
Wu et al. | A lateral flow biosensor for the detection of human pluripotent stem cells | |
CN103645314A (zh) | 能自动过滤样品的试条卡 | |
Wu et al. | Microfluidic technology for the isolation and analysis of exosomes | |
Ding et al. | Microfluidic-chip-integrated biosensors for lung disease models | |
Czaplicka et al. | Effect of varying expression of EpCAM on the efficiency of CTCs detection by SERS-based immunomagnetic optofluidic device | |
García-Hernández et al. | Optical detection of cancer cells using lab-on-a-chip | |
Bragina et al. | Highly sensitive nanomagnetic quantification of extracellular vesicles by immunochromatographic strips: A tool for liquid biopsy | |
CN107034191A (zh) | 一种在微流控芯片中利用透明质酸功能化的磁珠识别和分离循环肿瘤细胞的方法 | |
Onishi et al. | Application of magnetic nanoparticles for rapid detection and in situ diagnosis in clinical oncology | |
Yang et al. | Single step in situ detection of surface protein and microRNA in clustered extracellular vesicles using flow cytometry | |
Moyano et al. | Magnetic lateral flow immunoassay for small extracellular vesicles quantification: application to colorectal cancer biomarker detection |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information |
Address after: 266061 Songling Road, Laoshan District, Qingdao, Shandong Province, No. 99 Applicant after: Qingdao University Of Science And Technology Address before: School of chemistry and molecular engineering, Qingdao University of science and technology, No.53, Zhengzhou road, Shibei District, Qingdao, Shandong 266042 Applicant before: Qingdao University Of Science And Technology |
|
CB02 | Change of applicant information | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230104 Address after: 276000 0010 Hengchang Avenue, Qiaotou village, Chu Dun Town, Luozhuang District, Linyi City, Shandong Province Patentee after: Shandong blue Peng Intelligent Technology Co.,Ltd. Address before: 266061 Songling Road, Laoshan District, Qingdao, Shandong Province, No. 99 Patentee before: QINGDAO University OF SCIENCE AND TECHNOLOGY |
|
TR01 | Transfer of patent right |