CN110241150A - The amplification fermentation process of β -1,3- glucan - Google Patents
The amplification fermentation process of β -1,3- glucan Download PDFInfo
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Abstract
The present invention relates to the fermentation process more particularly to β -1 of a kind of sugar, the amplification fermentation process of 3- glucan belongs to technical field of microbial fermentation.The present invention passes through the control to inoculum concentration, concentration of substrate, fermentation time, N stress condition in strain fermentation incubation, directly using sucrose as carbon source, β -1 is synthesized by bioconversion, the technique of 3- glucan, through detecting Sucrose conversion > 68.2%, the yield > 38.4mg/mL of β -1,3- glucan.β -1, the industrialized production of 3- glucan are synthesized by the direct bioconversion that sucrose may be implemented in the technique.Final product beta-1,3-dextran is with a wide range of applications in industries such as daily use chemicals, environmental protection, food, agriculturals.
Description
Technical field
The present invention relates to the fermentation process more particularly to β -1 of a kind of sugar, the amplification fermentation process of 3- glucan belongs to micro-
Technical field of biological fermentation.
Background technique
β -1,3- glucan are a kind of backbone structures by β -1,3- glucosides key connection, usually contain different proportion and size
β -1,3, β-Isosorbide-5-Nitraes, β -1, the macromolecular polysaccharide of the branch of 6 glucosides key connections.In the 1960s, being just proved β -1,3 Portugals are poly-
Sugar has enhancing mammalian immune vigor, antitumor, antibacterium, antiviral, reduction cholesterol and blood lipid, promotes wound healing
The effects of, it is shown that it is widely paid close attention in the potential using value of pharmaceutical field；Recent studies indicate that β -1,3
Glucan is applied in daily chemical products, can be played moisturizing, anti-inflammatory, anti-aging, be gone wrinkle, anti-dandruff, removing jaundice, accelerate to repair, increase
Add the effect of skin elasticity.The immunity of organisms of livestock and poultry can be enhanced applied to livestock and poultry cultivation, improve gastrointestinal function, gradually to replace
Progradation is played for the abuse of antibiotic.β -1 is found simultaneously, and 3- glucan can be used as foliar fertilizer use, in wheat
In growth test, growing way is extremely excellent, moreover it is possible to eliminate some toxic of herbicide.To popularize green agriculture technology in an all-round way, into one
Step has expanded β -1,3 glucan application fields.
The method of traditional mode of production beta glucan is to extract to obtain from mushroom, yeast, oat, highland barley.Due to by raw material
Production scale, the limitation of production cycle produce the market demand that beta glucan is far from satisfying by extracting mode.In order to change
Into beta glucan production method, the yield of beta glucan is promoted, in recent years, has carried out high yield beta glucan in research both at home and abroad
The screening study of microorganism, filtered out at present be suitable for large-scale production beta glucan microorganism include brewer's yeast, it is withered
Careless bacillus etc., foreign countries have carried out β-Portugal by microbial fermentation culture large-scale production beta glucan bacterial origin
The trial of dextranase, but the correlative study of China's microbial fermentation production beta glucan is started late, based on brewer's yeast,
His source is less.Brewer's yeast produces beta glucan there are still raw material trans-utilization rate is low, and product purity is low, poor quality etc. lacks
Point, it is difficult to adapt to demand of the Vehicles Collected from Market to high-quality beta glucan.In view of the foregoing drawbacks, applicant was on July 19th, 2017
Apply " a kind of high yield β -1,3- glucan bacillus subtilis and its application ", application number: 201710590529.7, by straight
Connecing sucrose inversion is β -1, and 3- glucan realizes sucrose Efficient Conversion.It, may when production that application discloses experimental section
It will appear technological problems, a series of problems, such as low yield, how further to realize industrialized production using this method, yield is such as
What, still belongs to unknown.
Summary of the invention
The purpose of the present invention is cannot achieve industrial defect for of the existing technology, β -1 is proposed, the Portugal 3- is poly-
The amplification fermentation process of sugar further solves the problems, such as industrialized production.
The present invention solves technical problem by the following technical programs: the amplification fermentation process of β -1,3- glucan, including with
Lower step:
The first step, preparation strain activation and culture base, according to KH2PO40.3-1.0g/L；CaCl20.3-1.0g/L, MgSO4
0.6-1.2g/L, FeSO4The mass concentration of 7H2O 0.6-1.2g/L, sucrose 3.0-8.0g/L, agar 12.0-18.0g/L claim
Substance is taken, redistilled water dissolves constant volume, adjusts pH 6.5-7.0, and 115 DEG C sterilize 30 minutes；
Second step, actication of culture, by bacterial strain streak inoculation strain activation and culture base obtained by the first step, 25-30 DEG C of standing
Constant temperature incubation 2-3 days；
Third step prepares seed culture fluid, according to peptone 8.0-10.0g/L, yeast extract 3.0-5.0g/L, sodium chloride
The mass concentration of 3.0-5.0g/L weighs each substance, and redistilled water dissolves constant volume, adjusts culture solution pH 7.0-7.8,115-121 DEG C goes out
Bacterium 20-30 minutes；
4th step, culture seed liquor scrape activated spawn from strain activation and culture base and are transferred to the training of 100mL liquid seeds
In nutrient solution, with the speed shake culture 48-72h of 150-300rpm in 28-32 DEG C of shaking table, until culture solution OD600 value is in 0.6-
Between 0.9, the seed liquor as primary fermentation culture is spare；
5th step prepares primary fermentation culture solution, according to peptone 8.0-10.0g/L, yeast extract 3.0-5.0g/L, chlorine
The mass concentration for changing sodium 3.0-5.0g/L weighs each substance, and redistilled water dissolves constant volume, adjusts culture solution pH 7.0-7.8,115-121
DEG C sterilizing 20-30 minutes；
6th step, culture amplification fermentation seed liquid, according to seed liquor: primary fermentation culture solution (V/V) is than 1: 300-800
Seed liquor is inoculated into primary fermentation culture solution by ratio, in 25-35 DEG C of fermentor, is fermented 1-2 days, is adjusted pH 6.5-7.0, stir
Rate 150-200rpm, ventilatory capacity 0.4-0.5vvm are mixed, until fermentation culture OD600 value is greater than 0.9, as amplification fermented and cultured
Seed liquor it is spare；
7th step, preparation amplification fermentation culture, weigh, redistilled water dissolution is fixed according to the mass concentration of sucrose 120g/L
Hold, adjust culture solution pH 7.0-7.8, is passed through 115 DEG C of steam and sterilizes 30 minutes；
8th step, amplification fermented and cultured, according to primary fermentation culture solution: amplification fermentation culture (V/V) ratio 1: 300-800
Ratio, by primary fermentation liquid be inoculated into amplification fermentation culture in, in 25-35 DEG C of fermentor, ferment 5-9 days, wherein fermenting
It is 7.0 that pH, which need to be controlled within the 1-2 days, and error is no more than ± 0.1, stirring rate 150-200rpm, ventilatory capacity 0.4-0.5vvm；
Timing sampling detection adjusts pH to 5.5 when nitrogen source exhausts, and error is no more than ± 0.1, stirring rate 400-600rpm, ventilation
Amount is 0.7-0.8vvm, finally obtains β -1,3- glucan crude product.
In above method, the bacterial strain is the bacillus subtilis that deposit number is CGMCC No.12176
(Bucillussubtilis)。
It is beta-1,3-dextran that the fermentation liquid, which is by substrate sucrose inversion,.
The Sucrose conversion > 68.2% of the substrate, β -1, the yield > 38.4mg/mL of 3- glucan.
The present invention passes through the control to inoculum concentration, concentration of substrate, fermentation time, N stress condition in strain fermentation incubation
System synthesizes β -1, the technique of 3- glucan, through detecting Sucrose conversion > by bioconversion directly using sucrose as carbon source
The yield > 38.4mg/mL of 68.2%, β -1,3- glucan.The direct bioconversion synthesis of sucrose may be implemented by the technique
The industrialized production of beta-1,3-dextran.Final product beta-1,3-dextran has in industries such as daily use chemicals, environmental protection, food, agriculturals
Broad application prospect.
Detailed description of the invention
Fig. 1 is the HPLC map of fermentation liquid.
Specific embodiment
The fermentation liquid map of following embodiment as shown in Figure 1, glucose in figure, fructose, sucrose HPLC map (wherein A,
B is respectively standard items map and test liquid map).1,2,3 be respectively glucose, fructose and sucrose in figure.
Embodiment 1
(1) prepared by strain activation and culture base: according to KH2PO40.3g/L；CaCl20.3g/L, MgSO40.6g/L,
FeSO47H2O 0.6g/L, sucrose 5.0g/L, the mass concentration of agar 12.0g/L weigh substance, and redistilled water dissolves constant volume, adjust
PH 6.5-7.0,115 DEG C sterilize 30 minutes；(2) actication of culture: Bacillus subtilis strain is taken, streak inoculation is in actication of culture
Culture medium, 25 DEG C standing constant temperature incubation 2 days；(3) prepared by seed culture fluid: according to peptone 8.0g/L, yeast extract 3.0g/L,
The mass concentration of sodium chloride 3.0g/L weighs each substance, and redistilled water dissolves constant volume, adjusts 7.0,115 DEG C of culture solution pH to sterilize 30 points
Clock；(4) it seed culture: is transferred in 100mL liquid seeds culture solution from activated spawn is scraped on strain activation and culture base, in 28
With the speed shake culture 48h of 150rpm in DEG C shaking table, until culture solution OD600 value is greater than 0.7, the seed liquor as fermented and cultured
It is spare；(5) prepared by primary fermentation culture solution: the quality according to peptone 2.0g/L, yeast extract 1.5g/L, sucrose 120g/L is dense
Degree weighs each substance, and redistilled water dissolves constant volume, adjusts culture solution pH 7.0, is passed through 115 DEG C of steam and sterilizes 30 minutes；(6) primary hair
Ferment culture: according to seed liquor: primary fermentation culture solution (V/V) compares 1: 400 ratio, and seed liquor is inoculated into primary fermentation culture
It in liquid, in 30 DEG C of fermentor, stirring rate 150rpm, ventilatory capacity 0.4vvm, ferments 1 day, until culture solution OD600 value is greater than 0.9；
(7) amplification fermentation culture preparation: weighing according to the mass concentration of sucrose 120g/L, and redistilled water dissolves constant volume, adjusts culture solution pH
7.0, it is passed through 115 DEG C of steam and sterilizes 30 minutes；(8) amplify fermented and cultured: according to primary fermentation culture solution: amplification fermentation culture
(V/V) primary fermentation culture solution is inoculated into amplification fermentation culture by the ratio for comparing 1: 400, in 30 DEG C of fermentor, fermentation 7
It, wherein 1-2 days stirring rates of fermentation are 150rpm, ventilatory capacity 0.4vvm, when nitrogen source exhausts, tune stirring rate is
400rpm, ventilatory capacity 0.6vvm, obtain β -1, and 3- glucan crude yield is 39.4mg/mL.
Embodiment 2
(1) prepared by strain activation and culture base: according to KH2PO40.4g/L；CaCl20.4g/L, MgSO40.7g/L,
FeSO47H2O 0.8g/L, sucrose 6.0g/L, the mass concentration of agar 12.0g/L weigh substance, and redistilled water dissolves constant volume, adjust
7.0,115 DEG C of pH sterilize 30 minutes；(2) actication of culture: Bacillus subtilis strain is taken, streak inoculation is in strain activation and culture
Base, 25 DEG C standing constant temperature incubation 2 days；(3) prepared by seed culture fluid: according to peptone 9.0g/L, yeast extract 4.0g/L, chlorination
The mass concentration of sodium 4.0g/L weighs each substance, and redistilled water dissolves constant volume, adjusts 7.0,115 DEG C of culture solution pH to sterilize 30 minutes；
(4) it seed culture: is transferred in 100mL liquid seeds culture solution from activated spawn is scraped on strain activation and culture base, in 28 DEG C
With the speed shake culture 54h of 150rpm in shaking table, until culture solution OD600 value is greater than 0.8, the seed liquor as fermented and cultured is standby
With；(5) prepared by primary fermentation culture solution: according to peptone 2.0g/L, yeast extract 1.5g/L, the mass concentration of sucrose 120g/L
Each substance is weighed, redistilled water dissolves constant volume, adjusts culture solution pH 7.0, is passed through 115 DEG C of steam and sterilizes 30 minutes；(6) primary fermentation
Culture: according to seed liquor: primary fermentation culture solution (V/V) compares 1: 600 ratio, and seed liquor is inoculated into primary fermentation culture solution
In, it in 30 DEG C of fermentor, stirring rate 200rpm, ventilatory capacity 0.4vvm, ferments 1 day, until culture solution OD600 value is greater than 0.9；
(7) amplification fermentation culture preparation: weighing according to the mass concentration of sucrose 120g/L, and redistilled water dissolves constant volume, adjusts culture solution pH
7.0, it is passed through 115 DEG C of steam and sterilizes 30 minutes；(8) amplify fermented and cultured: according to primary fermentation culture solution: amplification fermentation culture
(V/V) primary fermentation culture solution is inoculated into amplification fermentation culture by the ratio for comparing 1: 600, in 30 DEG C of fermentor, fermentation 7
It, wherein 1-2 days stirring rates of fermentation are 200rpm, ventilatory capacity 0.4vvm, when nitrogen source exhausts, tune stirring rate is
500rpm, ventilatory capacity 0.6vvm, obtain β -1, and 3- glucan crude yield is 43.5mg/mL.
Embodiment 3
(1) prepared by strain activation and culture base: according to KH2PO40.4g/L；CaCl20.4g/L, MgSO40.7g/L,
FeSO47H2O 0.7g/L, sucrose 6.0g/L, the mass concentration of agar 12.0g/L weigh substance, and redistilled water dissolves constant volume, adjust
6.5,115 DEG C of pH sterilize 30 minutes；(2) actication of culture: Bacillus subtilis strain is taken, streak inoculation is in strain activation and culture
Base, 25 DEG C standing constant temperature incubation 2 days；(3) prepared by seed culture fluid: according to peptone 9.0g/L, yeast extract 4.0g/L, chlorination
The mass concentration of sodium 4.0g/L weighs each substance, and redistilled water dissolves constant volume, adjusts 7.0,115 DEG C of culture solution pH to sterilize 30 minutes；
(4) it seed culture: is transferred in 100mL liquid seeds culture solution from activated spawn is scraped on strain activation and culture base, in 28 DEG C
With the speed shake culture 48h of 150rpm in shaking table, until culture solution OD600 value is greater than 0.8, the seed liquor as fermented and cultured is standby
With；(5) prepared by primary fermentation culture solution: according to peptone 2.0g/L, yeast extract 1.5g/L, the mass concentration of sucrose 120g/L
Each substance is weighed, redistilled water dissolves constant volume, adjusts culture solution pH 7.0, is passed through 115 DEG C of steam and sterilizes 30 minutes；(6) primary fermentation
Culture: according to seed liquor: primary fermentation culture solution (V/V) compares 1: 500 ratio, and seed liquor is inoculated into primary fermentation culture solution
In, it in 30 DEG C of fermentor, stirring rate 200rpm, ventilatory capacity 0.5vvm, ferments 1 day, until culture solution OD600 value is greater than 0.9；
(7) amplification fermentation culture preparation: weighing according to the mass concentration of sucrose 120g/L, and redistilled water dissolves constant volume, adjusts culture solution pH
7.0, it is passed through 115 DEG C of steam and sterilizes 30 minutes；(8) amplify fermented and cultured: according to primary fermentation culture solution: amplification fermentation culture
(V/V) primary fermentation culture solution is inoculated into amplification fermentation culture by the ratio for comparing 1: 500, in 30 DEG C of fermentor, fermentation 7
It, wherein 1-2 days stirring rates of fermentation are 200rpm, ventilatory capacity 0.5vvm, when nitrogen source exhausts, tune stirring rate is
500rpm, ventilatory capacity 0.8vvm, obtain β -1, and 3- glucan crude yield is 49.7mg/mL.
In addition to above-mentioned implementation, the present invention can also have other embodiments.It is all to be formed using equivalent substitution or equivalent transformation
Technical solution, fall within the scope of protection required by the present invention.
Claims (4)
1. the amplification fermentation process of β -1,3- glucan, comprising the following steps:
The first step, preparation strain activation and culture base, according to KH2PO40.3-1.0g/L；CaCl20.3-1.0g/L, MgSO4 0.6-
1.2g/L, FeSO4The mass concentration of 7H2O 0.6-1.2g/L, sucrose 3.0-8.0g/L, agar 12.0-18.0g/L weigh object
Matter, redistilled water dissolve constant volume, adjust pH 6.5-7.0, and 115 DEG C sterilize 30 minutes；
Second step, actication of culture, by bacterial strain streak inoculation strain activation and culture base obtained by the first step, 25-30 DEG C of standing constant temperature
Culture 2-3 days；
Third step prepares seed culture fluid, according to peptone 8.0-10.0g/L, yeast extract 3.0-5.0g/L, sodium chloride 3.0-
The mass concentration of 5.0g/L weighs each substance, and redistilled water dissolves constant volume, adjusts culture solution pH 7.0-7.8,115-121 DEG C of sterilizing 20-
30 minutes；
4th step, culture seed liquor, scrape activated spawn from strain activation and culture base and are transferred to 100mL liquid seeds culture solution
In, with the speed shake culture 48-72h of 150-300rpm in 28-32 DEG C of shaking table, until culture solution OD600 value 0.6-0.9 it
Between, the seed liquor as primary fermentation culture is spare；
5th step prepares primary fermentation culture solution, according to peptone 8.0-10.0g/L, yeast extract 3.0-5.0g/L, sodium chloride
The mass concentration of 3.0-5.0g/L weighs each substance, and redistilled water dissolves constant volume, adjusts culture solution pH 7.0-7.8,115-121 DEG C goes out
Bacterium 20-30 minutes；
6th step, culture amplification fermentation seed liquid, according to seed liquor: primary fermentation culture solution (V/V) compares 1: 300-800 ratio
Example, seed liquor is inoculated into primary fermentation culture solution, in 25-35 DEG C of fermentor, is fermented 1-2 days, and pH 6.5-7.0, stirring are adjusted
Rate 150-200rpm, ventilatory capacity 0.4-0.5vvm, until fermentation culture OD600 value is greater than 0.9, as amplification fermented and cultured
Seed liquor is spare；
7th step, preparation amplification fermentation culture, weigh, redistilled water dissolves constant volume, adjusts according to the mass concentration of sucrose 120g/L
Culture solution pH 7.0-7.8 is passed through 115 DEG C of steam and sterilizes 30 minutes；
8th step, amplification fermented and cultured, according to primary fermentation culture solution: 1: 300-800 ratio is compared in amplification fermentation culture (V/V)
Primary fermentation liquid is inoculated into amplification fermentation culture by example, in 25-35 DEG C of fermentor, is fermented 5-9 days, wherein the 1-2 that ferments
It is 7.0 that it, which need to control pH, and error is no more than ± 0.1, stirring rate 150-200rpm, ventilatory capacity 0.4-0.5vvm；Periodically
Sample detection adjusts pH to 5.5 when nitrogen source exhausts, and error is no more than ± 0.1, stirring rate 400-600rpm, and ventilatory capacity is
0.7-0.8vvm finally obtains β -1,3- glucan crude product.
2. the amplification fermentation process of β -1 according to claim 1,3- glucan, it is characterised in that: the bacterial strain is deposit number
For the bacillus subtilis (Bucillussubtilis) of CGMCC No.12176.
3. the amplification fermentation process of β -1 according to claim 1,3- glucan, it is characterised in that: the fermentation liquid is the bottom of by
Object sucrose inversion is beta-1,3-dextran.
4. the amplification fermentation process of β -1 according to claim 3,3- glucan, it is characterised in that: the sucrose of the substrate turns
Rate > 68.2%, β -1, the yield > 38.4mg/mL of 3- glucan.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267420A (en) * | 2017-07-19 | 2017-10-20 | 南京曜动节能环保科技有限公司 | A kind of glucan bacillus subtilises of high yield β 1,3 and its application |
| CN111773239A (en) * | 2020-07-31 | 2020-10-16 | 四川合泰新光生物科技有限公司 | Application of beta-1, 3-glucan in preparation of medicines and daily chemical products for repairing skin injuries |
| CN111778300A (en) * | 2020-07-31 | 2020-10-16 | 四川合泰新光生物科技有限公司 | Beta-1, 3-glucan and preparation method and application thereof |
| CN112646846A (en) * | 2020-12-23 | 2021-04-13 | 山东国力生物科技有限公司 | Method for producing beta-1, 3-glucan by using non-growth coupling characteristic of bacteria |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226159A (en) * | 2010-11-29 | 2011-10-26 | 山东大学 | Strain of Enterobacter cloacae and its application in the preparation of 2,3-butylene glycol |
| CN103330258A (en) * | 2013-07-17 | 2013-10-02 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN103609864A (en) * | 2013-11-29 | 2014-03-05 | 浙江科峰生物技术有限公司 | Method for producing bean pulp feed additive |
| CN103667387A (en) * | 2013-11-27 | 2014-03-26 | 陕西科技大学 | Method for separation and extraction of epothilone B from sorangium cellulosum fermentation liquid |
| CN103773722B (en) * | 2014-01-16 | 2015-08-19 | 中国药科大学 | Salt tolerant also has subtilis and the application thereof of low-temperature biological deamination function |
| CN104928202A (en) * | 2014-03-21 | 2015-09-23 | 中国科学院沈阳应用生态研究所 | Fermentation culture method of bacillus |
| CN107267420A (en) * | 2017-07-19 | 2017-10-20 | 南京曜动节能环保科技有限公司 | A kind of glucan bacillus subtilises of high yield β 1,3 and its application |
-
2019
- 2019-06-25 CN CN201910554477.7A patent/CN110241150A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226159A (en) * | 2010-11-29 | 2011-10-26 | 山东大学 | Strain of Enterobacter cloacae and its application in the preparation of 2,3-butylene glycol |
| CN103330258A (en) * | 2013-07-17 | 2013-10-02 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN103667387A (en) * | 2013-11-27 | 2014-03-26 | 陕西科技大学 | Method for separation and extraction of epothilone B from sorangium cellulosum fermentation liquid |
| CN103609864A (en) * | 2013-11-29 | 2014-03-05 | 浙江科峰生物技术有限公司 | Method for producing bean pulp feed additive |
| CN103773722B (en) * | 2014-01-16 | 2015-08-19 | 中国药科大学 | Salt tolerant also has subtilis and the application thereof of low-temperature biological deamination function |
| CN104928202A (en) * | 2014-03-21 | 2015-09-23 | 中国科学院沈阳应用生态研究所 | Fermentation culture method of bacillus |
| CN107267420A (en) * | 2017-07-19 | 2017-10-20 | 南京曜动节能环保科技有限公司 | A kind of glucan bacillus subtilises of high yield β 1,3 and its application |
Non-Patent Citations (2)
| Title |
|---|
| 李学如: "《发酵工艺原理与技术》", 31 August 2014 * |
| 江宁: "《微生物生物技术》", 31 May 2008 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267420A (en) * | 2017-07-19 | 2017-10-20 | 南京曜动节能环保科技有限公司 | A kind of glucan bacillus subtilises of high yield β 1,3 and its application |
| CN107267420B (en) * | 2017-07-19 | 2020-06-19 | 南京曜动节能环保科技有限公司 | High-yield β -1, 3-glucan bacillus subtilis and application thereof |
| CN111773239A (en) * | 2020-07-31 | 2020-10-16 | 四川合泰新光生物科技有限公司 | Application of beta-1, 3-glucan in preparation of medicines and daily chemical products for repairing skin injuries |
| CN111778300A (en) * | 2020-07-31 | 2020-10-16 | 四川合泰新光生物科技有限公司 | Beta-1, 3-glucan and preparation method and application thereof |
| CN112646846A (en) * | 2020-12-23 | 2021-04-13 | 山东国力生物科技有限公司 | Method for producing beta-1, 3-glucan by using non-growth coupling characteristic of bacteria |
| CN112646846B (en) * | 2020-12-23 | 2023-01-31 | 山东国力生物科技有限公司 | Method for producing beta-1,3-glucan by using non-growth coupling characteristic of bacteria |
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