CN110237191A - A kind of compound dendrobium officinale ferment and its preparation process - Google Patents
A kind of compound dendrobium officinale ferment and its preparation process Download PDFInfo
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- CN110237191A CN110237191A CN201910691796.2A CN201910691796A CN110237191A CN 110237191 A CN110237191 A CN 110237191A CN 201910691796 A CN201910691796 A CN 201910691796A CN 110237191 A CN110237191 A CN 110237191A
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- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 76
- 150000001875 compounds Chemical class 0.000 title claims abstract description 76
- 238000002360 preparation method Methods 0.000 title claims abstract description 52
- 239000007788 liquid Substances 0.000 claims abstract description 118
- 239000000463 material Substances 0.000 claims abstract description 96
- 238000000855 fermentation Methods 0.000 claims abstract description 56
- 230000004151 fermentation Effects 0.000 claims abstract description 56
- 239000006228 supernatant Substances 0.000 claims abstract description 8
- 238000000926 separation method Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 239000012530 fluid Substances 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 238000005352 clarification Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 3
- 241000026010 Dendrobium candidum Species 0.000 abstract description 16
- 235000003140 Panax quinquefolius Nutrition 0.000 abstract description 16
- 240000005373 Panax quinquefolius Species 0.000 abstract description 15
- 230000000694 effects Effects 0.000 abstract description 7
- 230000035764 nutrition Effects 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 235000008216 herbs Nutrition 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 20
- RYAHJFGVOCZDEI-UFFNCVEVSA-N Dendrobine Chemical compound C([C@H]1CC[C@@H]2[C@@]31C)N(C)[C@@H]3[C@H]1[C@@H](C(C)C)[C@@H]2C(=O)O1 RYAHJFGVOCZDEI-UFFNCVEVSA-N 0.000 description 12
- RYAHJFGVOCZDEI-CZKZLRAZSA-N dendrobine Natural products O=C1O[C@@H]2[C@H](C(C)C)[C@H]1[C@H]1[C@@]3(C)[C@@H]2N(C)C[C@H]3CC1 RYAHJFGVOCZDEI-CZKZLRAZSA-N 0.000 description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 239000003814 drug Substances 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 8
- 239000013558 reference substance Substances 0.000 description 8
- 239000002994 raw material Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000007789 sealing Methods 0.000 description 5
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 4
- 240000004638 Dendrobium nobile Species 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
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- 239000003513 alkali Substances 0.000 description 4
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- 235000013305 food Nutrition 0.000 description 4
- 235000019253 formic acid Nutrition 0.000 description 4
- 230000001953 sensory effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
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- 235000019441 ethanol Nutrition 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
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- 239000012528 membrane Substances 0.000 description 2
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
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- 206010030113 Oedema Diseases 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
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- 239000010839 body fluid Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
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- 238000004519 manufacturing process Methods 0.000 description 1
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- 210000000697 sensory organ Anatomy 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The embodiment of the present application provides a kind of compound dendrobium officinale ferment and its preparation process, is related to Application to the Chinese medical herbs field.The compound dendrobium officinale ferment of the embodiment of the present application is made medicinal material decocting liquid using medicinal material and ferments to obtain again, and according to parts by weight, medicinal material includes: 15-30 parts of dendrobium candidum, 15-30 parts of American Ginseng, 10-20 parts of rhizoma polygonati and 10-20 parts of dried orange peel.The preparation process of compound dendrobium officinale ferment is to be uniformly mixed medicinal material decocting liquid and brown sugar by medicinal material and brown sugar mass ratio 40-135:15-30, and ferment 30-60d under conditions of fermentation temperature is 20-35 DEG C, is separated by solid-liquid separation to obtain supernatant liquid.The embodiment of the present application is designed to provide a kind of compound dendrobium officinale ferment and its preparation process, and mouthfeel is good, and nutrition and effect are abundant.
Description
Technical field
This application involves Application to the Chinese medical herbs fields, in particular to a kind of compound dendrobium officinale ferment and its preparation work
Skill.
Background technique
Ferment refers to animal, plant and mushroom etc. for raw material, through containing particular organisms activity made from microbial fermentation
The product of ingredient.Have the ferment product prepared using Chinese medicine as fermenting raw materials in the prior art, but it is existing with Chinese medicine
Material is poor as ferment mouthfeel made from raw material, and people are difficult to receive.And the Chinese medicine that existing ferment uses is single, causes
It nutrition and has a single function, it is difficult to meet the needs of people, or the Chinese medicine type used is too many, but nutrition and function are still difficult
To meet the needs of people, the waste of raw material is caused.
Summary of the invention
The embodiment of the present application is designed to provide a kind of compound dendrobium officinale ferment and its preparation process, and mouthfeel is good, battalion
It supports and effect is abundant.
In a first aspect, the embodiment of the present application provides a kind of compound dendrobium officinale ferment, use medicinal material that medicinal material water is made
Decocting liquid ferments to obtain again, and according to parts by weight, medicinal material includes:
In the above-mentioned technical solutions, compound dendrobium officinale ferment is that medicinal material decocting liquid is made using medicinal material to ferment to obtain again,
Inventor has found during realizing the application: determining that a very important aspect of ferment function is whether it uses section
Formula is learned, is not that raw material type is The more the better, raw material type is more, and demand threshold is not achieved in single active constituent, is equal to not
There is corresponding function.The application uses medicinal material: medicinal material decocting liquid is made as raw material in dendrobium candidum, American Ginseng, rhizoma polygonati, dried orange peel, then
Fermentation obtains compound dendrobium officinale ferment, can not only retain the effective component in medicinal material, and can also obtain active constituent, product
Mouthfeel is good, and nutrition and effect are abundant, has Yin-nourishing and body fluid promoting, the effect of strengthen immunity.
In one possible implementation, the medicinal material concentration of medicinal material decocting liquid is 0.8-1.5g/ml.
It in the above-mentioned technical solutions, is that the medicinal material decocting liquid that 0.8-1.5g/ml is obtained is sent out than convenient according to medicinal material concentration
Ferment, and smell is smaller, flavour is relatively good, and the corresponding compound dendrobium officinale ferment obtained that ferments is in good taste.
Second aspect, the embodiment of the present application provide a kind of preparation process of the compound dendrobium officinale ferment of first aspect,
Itself the following steps are included:
Medicinal material decocting liquid and brown sugar are uniformly mixed by medicinal material and brown sugar mass ratio 40-135:15-30, are in fermentation temperature
Ferment 30-60d under conditions of 20-35 DEG C, is separated by solid-liquid separation to obtain supernatant liquid.
In the above-mentioned technical solutions, medicinal material decocting liquid and brown sugar are carried out by medicinal material and brown sugar mass ratio 40-135:15-30
Fermentation sufficiently can carry out fermentation to medicinal material decocting liquid and obtain active constituent, and send out under conditions of fermentation temperature is 20-35 DEG C
Ferment 30-60d, energy basic guarantee fermentation are complete.
In one possible implementation, the medicinal material concentration of medicinal material decocting liquid is 0.8-1.5g/ml, before fermentation, often
The water of 110-150mL is additionally added in 50-90mL medicinal material decocting liquid；Optionally, the corresponding medicinal material water of every 50-90mL medicinal material decocting liquid
The total volume of decocting liquid and water is 180-220mL.
In the above-mentioned technical solutions, it when the medicinal material concentration of medicinal material decocting liquid is 0.8-1.5g/ml, needs in medicinal material decocting liquid
Middle addition suitable quantity of water is for diluting medicinal material decocting liquid, to guarantee efficient, sufficient fermentation；On this basis, every 50-90mL medicine
The total volume of the corresponding medicinal material decocting liquid of material decocting liquid and water is 180-220mL, and the medicinal material decocting liquid water content after dilution compares
Suitably, being further ensured that efficiently, adequately to ferment.
In one possible implementation, fermentation temperature is 25-30 DEG C, when fermentation a length of 40-50d.
In the above-mentioned technical solutions, ferment 40-50d under conditions of fermentation temperature is 25-30 DEG C, can basic guarantee hair
Ferment is complete.
In one possible implementation, the preparation method of medicinal material decocting liquid is the following steps are included: add water logging for medicinal material
20-40min is steeped, is added water, under stirring, 20-40min is kept after being heated to boiling, filters out medical fluid, repeat decoction extremely
It is few primary, merge each medical fluid, obtains medicinal material decocting liquid.
In the above-mentioned technical solutions, a period of time is soaked in water, adds water and boils a period of time, and repeats to decoct at least one
It is secondary, sufficiently the effective component in medicinal material can be extracted, and the activity of effective component will not be destroyed.
In one possible implementation, it is fermented by the way of sealing and fermenting；During the fermentation, Mei Geyi
The section time mixes again, and gas is discharged.
In the above-mentioned technical solutions, it is fermented by the way of sealing and fermenting, is avoided that living contaminants, and guarantee fermentation
Quickly carry out；At regular intervals, discharge fermentation generates gas.
In one possible implementation, the method for separation of solid and liquid are as follows: stand the fermentation liquid after fermentation to clear naturally
Clearly, and centrifugal treating is carried out, collects supernatant.
In the above-mentioned technical solutions, using clarification is first stood naturally, the mode being centrifuged afterwards can obtain ferment, and remove
Impurity etc. is not suitable for edible substance.
In one possible implementation, the condition of centrifugal treating are as follows: revolving speed 1500-2000rpm, time 10-
20min。
In the above-mentioned technical solutions, 10-20min is centrifuged using the revolving speed of 1500-2000rpm, can made in fermentation liquid
Impurity is precipitated, thus the reservation effective component and active constituent of high degree.
Detailed description of the invention
Technical solution in ord to more clearly illustrate embodiments of the present application will make below to required in the embodiment of the present application
Attached drawing is briefly described, it should be understood that the following drawings illustrates only some embodiments of the application, therefore should not be seen
Work is the restriction to range, for those of ordinary skill in the art, without creative efforts, can be with
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is reference substance chromatogram；
Fig. 2 is the medicinal material decocting liquid chromatogram of embodiment 15；
Fig. 3 is blank control group chromatogram；
Fig. 4 is the compound dendrobium officinale ferment chromatogram of embodiment 15.
Specific embodiment
It, below will be in the embodiment of the present application to keep the purposes, technical schemes and advantages of the embodiment of the present application clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
The compound dendrobium officinale ferment and its preparation process of the embodiment of the present application are specifically described below.
The embodiment of the present application provides a kind of compound dendrobium officinale ferment, uses medicinal material that medicinal material decocting liquid is made and ferments again
It obtains, according to parts by weight, medicinal material includes: 15-30 parts of dendrobium candidum, 15-30 parts of American Ginseng, 10-20 parts of rhizoma polygonati and dried orange peel
10-20 parts.Illustratively, medicinal material includes 15 parts, 20 parts, 25 parts or 30 parts of dendrobium candidum or aforementioned any two adjacent values
Between any value number；Appointing between 15 parts, 20 parts, 25 parts or 30 parts of American Ginseng or aforementioned any two adjacent values
One numerical value number；Any value number between 10 parts, 15 parts or 20 parts of rhizoma polygonati or aforementioned any two adjacent values；And
Any value number between 10 parts, 15 parts or 20 parts of dried orange peel or aforementioned any two adjacent values.
In the present embodiment, the medicinal material concentration of medicinal material decocting liquid is 0.8-1.5g/ml, i.e. medicinal material decocting liquid is dense according to medicinal material
0.8-1.5g/ml is spent, adds water to cook to obtain using medicinal material.Illustratively, the medicinal material concentration of medicinal material decocting liquid is 0.8g/ml, 1g/
Ml, 1.3g/ml or 1.5g/ml.
The embodiment of the present application also provides a kind of preparation processes of above-mentioned compound dendrobium officinale ferment comprising following step
It is rapid:
Medicinal material decocting liquid and brown sugar are uniformly mixed by step (1) by medicinal material and brown sugar mass ratio 40-135:15-30, are being sent out
Ferment temperature is spontaneous fermentation 30-60d under conditions of 20-35 DEG C, and optionally, fermentation temperature is 25-30 DEG C, when fermentation a length of 40-
50d obtains fermentation liquid.
In the present embodiment, the corresponding decocting liquid of every kind of medicinal material can first be prepared separately, taken according still further to the consumption proportion of medicinal material
The decocting liquid of every kind of medicinal material is mixed to get medicinal material decocting liquid, first can also be used to match to take medicinal material not of the same race according to medicinal material, will
Medicinal material, which mixes, is prepared medicinal material decocting liquid.Illustratively, the preparation method of medicinal material decocting liquid will be the following steps are included: will
Medicinal material soaks in water 20-40min, adds water, and under stirring, keeps 20-40min after being heated to boiling, filters out medical fluid, then
It repeats to decoct at least once, i.e., keeps 10-30min after adding water to be heated to boiling the dregs of a decoction, filter out medical fluid, merge each medicine
Liquid needs to carry out drying concentration in 40-60 DEG C, obtains medicinal material decocting liquid in the case where amount of water is bigger.
In the present embodiment, the medicinal material concentration of medicinal material decocting liquid is 0.8-1.5g/ml, before fermentation, every 50-90mL medicinal material water
The water of 110-150mL is additionally added in decocting liquid；Optionally, the corresponding medicinal material decocting liquid of every 50-90mL medicinal material decocting liquid and water is total
Volume is 180-220mL.
In the present embodiment, fermented by the way of sealing and fermenting；During the fermentation, it mixes again at regular intervals
It is even, and gas is discharged.
Step (2), by fermentation liquid be separated by solid-liquid separation supernatant liquid to get to compound dendrobium officinale ferment.
In the present embodiment, the method for separation of solid and liquid are as follows: the fermentation liquid after fermentation is stood to clarification naturally, and be centrifuged
Supernatant is collected in processing.Optionally, the condition of centrifugal treating are as follows: revolving speed 1500-2000rpm, time 10-20min.
The feature of the application and performance are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of compound dendrobium officinale ferment, preparation method is as follows:
Dendrobium candidum, American Ginseng, rhizoma polygonati, dried orange peel are purchased, it is selected, it is prepared as decocting liquid respectively, and adjust concentration to suitable
In amount containing crude drug be 1g/ml.Each decocting liquid preparation flow: taking every kind of medicinal material to be placed in container in right amount, soak in water 30min,
Amount of water requires more than powder 5cm, keeps 30min after being heated to boiling, stirring when taking in boiling process filters out medical fluid, in medicine
It is added water in slag and keeps 20min after being heated to boiling in right amount, filter out medical fluid, merge medical fluid twice, being dried to concentration with 50 DEG C is
The corresponding medicinal material decocting liquid of 1g/ml is put into 4 DEG C of refrigerators and saves backup.
250ml triangular flask is subjected to high pressure sterilization processing (121 DEG C, 30min), dendrobium candidum, the West are pressed in triangular flask
It is ginseng, the volumetric usage ratio 4:4:3:3 addition dendrobium candidum decocting liquid of rhizoma polygonati and dried orange peel, American Ginseng decocting liquid, rhizoma polygonati decocting liquid, old
The total 50ml of severe edema due to hypofunction of the spleen decocting liquid is (correspondingly, dendrobium candidum decocting liquid about 14ml, American Ginseng decocting liquid about 14ml, rhizoma polygonati decocting liquid are about
11ml, dried orange peel decocting liquid about 11ml), supplement pure water to total volume is 200ml and brown sugar 25g, 1/3 space is reserved, with sterile
Container closure film (gas can be discharged, and the outer miscellaneous bacteria of container can not enter) sealing.
The triangular flask of sealing is placed in incubator in 25 DEG C of fermentation 40d, daily shaking flask 2 times (200rpm, 20s) makes to mix
It closes object sufficiently to ferment, and sees whether the case where living contaminants occur.
Compound dendrobium officinale ferment after fermentation is stood naturally to clarification, and does centrifugal treating (2000rpm, 15min),
Supernatant is collected, compound dendrobium officinale ferment is obtained.
Embodiment 2
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 1
Together, the difference is that:
In the present embodiment, the total 60ml (phase of dendrobium candidum decocting liquid, American Ginseng decocting liquid, rhizoma polygonati decocting liquid, dried orange peel decocting liquid
It answers, dendrobium candidum decocting liquid about 17ml, American Ginseng decocting liquid about 17ml, rhizoma polygonati decocting liquid about 13ml, dried orange peel decocting liquid are about
13ml), compound dendrobium officinale ferment is finally obtained.
Embodiment 3
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 1
Together, the difference is that:
In the present embodiment, the total 70ml (phase of dendrobium candidum decocting liquid, American Ginseng decocting liquid, rhizoma polygonati decocting liquid, dried orange peel decocting liquid
It answers, dendrobium candidum decocting liquid about 20ml, American Ginseng decocting liquid about 20ml, rhizoma polygonati decocting liquid about 15ml, dried orange peel decocting liquid are about
15ml), compound dendrobium officinale ferment is finally obtained.
Embodiment 4
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 1
Together, the difference is that:
In the present embodiment, the total 80ml (phase of dendrobium candidum decocting liquid, American Ginseng decocting liquid, rhizoma polygonati decocting liquid, dried orange peel decocting liquid
It answers, dendrobium candidum decocting liquid about 23ml, American Ginseng decocting liquid about 23ml, rhizoma polygonati decocting liquid about 17ml, dried orange peel decocting liquid are about
17ml), compound dendrobium officinale ferment is finally obtained.
Embodiment 5
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 1
Together, the difference is that:
In the present embodiment, the total 90ml (phase of dendrobium candidum decocting liquid, American Ginseng decocting liquid, rhizoma polygonati decocting liquid, dried orange peel decocting liquid
It answers, dendrobium candidum decocting liquid about 26ml, American Ginseng decocting liquid about 26ml, rhizoma polygonati decocting liquid about 19ml, dried orange peel decocting liquid are about
19ml), compound dendrobium officinale ferment is finally obtained.
Embodiment 6
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
The additive amount of brown sugar is 15g, finally obtains compound dendrobium officinale ferment.
Embodiment 7
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
The additive amount of brown sugar is 20g, finally obtains compound dendrobium officinale ferment.
Embodiment 8
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
The additive amount of brown sugar is 30g, finally obtains compound dendrobium officinale ferment.
Embodiment 9
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
Fermentation temperature is 20 DEG C, finally obtains compound dendrobium officinale ferment.
Embodiment 10
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
Fermentation temperature is 30 DEG C, finally obtains compound dendrobium officinale ferment.
Embodiment 11
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
Fermentation temperature is 35 DEG C, finally obtains compound dendrobium officinale ferment.
Embodiment 12
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
Fermentation time is 30d, finally obtains compound dendrobium officinale ferment.
Embodiment 13
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
Fermentation time is 50d, finally obtains compound dendrobium officinale ferment.
Embodiment 14
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
Fermentation time is 60d, finally obtains compound dendrobium officinale ferment.
Embodiment 15
The present embodiment provides a kind of compound dendrobium officinale ferment, the preparation method substantially phases of preparation method and embodiment 3
Together, the difference is that:
Brown sugar additive amount is 25g, and fermentation temperature is 25 DEG C, finally obtains compound dendrobium officinale ferment.
The product of the embodiment of the present application is evaluated and tested and analyzed below by way of test.
One, compound dendrobium officinale ferment sense organ is evaluated and tested
It is evaluated using compound dendrobium officinale ferment of the sensory evaluation scores method to embodiment 1- embodiment 15, referring to " GB/
T 5323-2018 " (plant enzyme), " GB/T 5324-2018 " (ferment product classification directive/guide), " GB/T10220-2012 " (sense
The introduction of official's analysis method), with five color of compound dendrobium officinale ferment, tissue morphology, flavour, smell, impurity index systems
Determine hundred-mark system sensory evaluation scores table, see the table below 1.
1 compound dendrobium officinale ferment sensory evaluation scores table of table
Each embodiment repeats parallel 3 samples, presses " GB/T16291.1-2012 " (organoleptic analysis-choice, training and pipe
Manage the general directive/guide of valuation officer) train 10 people to evaluate group, group is made of 4 males that the age is 18-45 years old with 6 women,
It scores the compound dendrobium officinale ferment of 3 samples of each embodiment, is averaged, the results are shown in Table 2.
2 compound dendrobium officinale ferment sensory score result of table
| Compound dendrobium officinale ferment | Scoring |
| Embodiment 1 | 57.12 |
| Embodiment 2 | 63.15 |
| Embodiment 3 | 86.48 |
| Embodiment 4 | 80.71 |
| Embodiment 5 | 69.34 |
| Embodiment 6 | 72.94 |
| Embodiment 7 | 89.35 |
| Embodiment 8 | 69.74 |
| Embodiment 9 | 66.15 |
| Embodiment 10 | 83.47 |
| Embodiment 11 | 63.61 |
| Embodiment 12 | 73.28 |
| Embodiment 13 | 79.75 |
| Embodiment 14 | 68.96 |
| Embodiment 15 | 91.67 |
Two, the general physical and chemical index of compound dendrobium officinale ferment and the measurement of characteristic physical and chemical index
6 parts of compound dendrobium officinale ferment made from Example 15, respectively according to " GB 5009.237-2016 " (food pH
The measurement of value), " GB 5009.225-2016 " (measurement of concentration of alcohol in wine) measures general physical and chemical index pH value and ethyl alcohol is dense
Degree, respectively according to " GB 12456-2008 " (measurement of total acid in food), " GB 5009.157-2016 " (organic acid in food
Measurement), " SN/T 4260-2015 " (measurements of Thick many candies in outlet plant derived foods) measurement total acid, organic acid and thick more
Sugar.Measurement result see the table below 3.
3 compound dendrobium officinale ferment physical and chemical index measurement result of table
As shown in Table 3, compound dendrobium officinale ferment indices made from the present embodiment comply with the national standard requirements, tool
Body is that general physical and chemical index (pH value, acetic acid content) and characteristic physical and chemical index (total acid, organic acid, Thick many candies) meet country
Standard requirements.
Three, dendrobine fermentation front and back assay in compound dendrobium officinale ferment
1. 6 parts of samples of compound dendrobium officinale ferment made from Example 15 are experimental group；There is equal volume medicine to be added
Material decocting liquid, but it is added without brown sugar, it gives same fermentation total volume, temperature and fermentation duration and handles to obtain compound dendrobium officinale ferment
Element, taking 6 parts of samples is control group；Medicinal material decocting liquid separately is replaced with 6 parts of pure water, isodose brown sugar is added, it is total to give same fermentation
The sample that volume, fermentation temperature and fermentation duration are handled is blank control group.
2. measuring dendrobine content method using gas chromatography (GC):
Chromatographic condition: chromatographic column: DB-1 capillary column (column length 30m, internal diameter 0.25mm, 0.25 μm of film thickness)；Temperature programming
Condition: 80 DEG C of initial temperature, 10 DEG C per minute rise to 250 DEG C, are kept for 5 minutes, injection port with 250 DEG C of detector temperature.
Reference substance preparation: naphthalene reference substance is taken, the naphthalene inner mark solution of 25 μ g/ml is prepared as with methanol；Take dendrobine reference substance
Solution is prepared as the dendrobine reference substance solution of 50 μ g/ml with methanol.Naphthalene inner mark solution and dendrobine reference substance solution are mixed,
Concentration be respectively 5 μ g/ml (how), 20 μ g/ml (dendrobine), obtain reference substance, draw 1 μ l and inject gas chromatograph, calculate correction
The factor.
3. to medicinal material decocting liquid dendrobine assay: taking medicinal material decocting liquid 1.0ml, set in round-bottomed flask, be added
0.05% formic acid solution 25ml, weighing, is heated to reflux 3h, lets cool, and weighs, and mends weight with 0.05% formic acid, is filtered with 0.22 μm of filter membrane
It crosses.Draw filtrate 2ml, be placed in 5ml volumetric flask, be added how inner mark solution 1ml, graduation mark is added to methanol, mix, draw 1 μ
L, with gas chromatograph for determination.
Fig. 1 is reference substance chromatogram；Fig. 2 is medicinal material decocting liquid chromatogram.
It is calculated by testing result, medicinal material decocting liquid dendrobium nobile alkali content is 158.82 μ g/ml.
4. to the compound dendrobium officinale ferment dendrobine assay of experimental group and control group: taking compound dendrobium officinale ferment
1.5ml is set in round-bottomed flask, and 0.05% formic acid solution 25ml is added, and weighing is heated to reflux 3h, lets cool, and is weighed, with 0.05%
Formic acid mends weight, is filtered with 0.22 μm of filter membrane.Draw filtrate 2ml, be placed in 5ml volumetric flask, be added how inner mark solution 1ml, with first
Alcohol adds to graduation mark, mixes, and 1 μ l is drawn, with gas chromatograph for determination.
Fig. 3 is blank control group chromatogram；Fig. 4 is compound dendrobium officinale ferment chromatogram.
It is calculated by testing result, each sample dendrobine content such as the following table 4.
Each sample of table 4 fermentation front and back dendrobine changes of contents (n=6, μ g/ml)
As shown in Table 4, blank control group (pure water+brown sugar) does not detect dendrobine before and after fermentation, shows to be not added with
Medicinal material decocting liquid is then generated without dendrobine；Dendrobium nobile alkali concentration is kept experimental group (medicinal material decocting liquid+brown sugar) substantially before and after fermentation
It is constant, show that medicinal material decocting liquid ingredient can be effectively retained in fermented rear ferment；Control group (medicinal material decocting liquid+pure water, total volume
Dendrobium nobile alkali concentration is held essentially constant on an equal basis) before and after fermentation, shows fermentation system, fermentation temperature etc. to dendrobium nobile alkali content without aobvious
Writing influences.
In conclusion the compound dendrobium officinale ferment and its preparation process of the embodiment of the present application, mouthfeel is good, nutrition and effect
It is abundant.
The above description is only an example of the present application, the protection scope being not intended to limit this application, for ability
For the technical staff in domain, various changes and changes are possible in this application.Within the spirit and principles of this application, made
Any modification, equivalent substitution, improvement and etc. should be included within the scope of protection of this application.
Claims (9)
1. a kind of compound dendrobium officinale ferment, which is characterized in that it uses the obtained medicinal material decocting liquid of medicinal material to ferment to obtain again, by weight
Number meter is measured, the medicinal material includes:
2. compound dendrobium officinale ferment according to claim 1, which is characterized in that the medicinal material concentration of the medicinal material decocting liquid
For 0.8-1.5g/ml.
3. a kind of preparation process of compound dendrobium officinale ferment as described in claim 1, which is characterized in that it includes following step
It is rapid:
The medicinal material decocting liquid and brown sugar are uniformly mixed by the medicinal material and the brown sugar mass ratio 40-135:15-30, sent out
Ferment temperature is fermented 30-60d under conditions of being 20-35 DEG C, is separated by solid-liquid separation to obtain supernatant liquid.
4. the preparation process of compound dendrobium officinale ferment according to claim 1, which is characterized in that the medicinal material decocting liquid
Medicinal material concentration be 0.8-1.5g/ml be additionally added 110-150mL's in medicinal material decocting liquid described in every 50-90mL before fermentation
Water；Optionally, the total volume of the corresponding medicinal material decocting liquid of medicinal material decocting liquid described in every 50-90mL and the water is 180-
220mL。
5. the preparation process of compound dendrobium officinale ferment according to claim 1, which is characterized in that the fermentation temperature is
25-30 DEG C, when fermentation a length of 40-50d.
6. the preparation process of compound dendrobium officinale ferment according to claim 1, which is characterized in that the medicinal material decocting liquid
Preparation method the following steps are included: the medicinal material is soaked in water 20-40min, add water, under stirring, be heated to
20-40min is kept after boiling, filters out medical fluid, is repeated decoction at least once, is merged each medical fluid, obtain medicinal material decocting liquid.
7. the preparation process of compound dendrobium officinale ferment according to claim 1, which is characterized in that using what is be sealed by fermentation
Mode is fermented；During the fermentation, it mixes again at regular intervals, and gas is discharged.
8. the preparation process of compound dendrobium officinale ferment according to claim 1, which is characterized in that the method for separation of solid and liquid
Are as follows: the fermentation liquid after fermentation is stood to clarification naturally, and carry out centrifugal treating, collects supernatant.
9. the preparation process of compound dendrobium officinale ferment according to claim 8, which is characterized in that the centrifugal treating
Condition are as follows: revolving speed 1500-2000rpm, time 10-20min.
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