CN110231323A - Hair extracting solution, preparation method and application and illicit drugs inspection method - Google Patents
Hair extracting solution, preparation method and application and illicit drugs inspection method Download PDFInfo
- Publication number
- CN110231323A CN110231323A CN201910603285.0A CN201910603285A CN110231323A CN 110231323 A CN110231323 A CN 110231323A CN 201910603285 A CN201910603285 A CN 201910603285A CN 110231323 A CN110231323 A CN 110231323A
- Authority
- CN
- China
- Prior art keywords
- hair
- extracting solution
- sample
- tested
- hair extracting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Abstract
The present invention provides a kind of hair extracting solution, preparation method and application and illicit drugs inspection methods, it is related to rapid detection technical field, hair extracting solution provided by the invention mainly consist of the following components: the triton x-100 and 50-200 μ g/ml Proteinase K that SDS that 100-200mM NaCl, 0.1-2mM CaCl, mass concentration are 0.1%-1%, volumetric concentration are 0.01%-0.1%, solvent is PBS solution.Hair extracting solution provided by the invention is cooperated by each specific components of certain concentration, can effectively detect the drugs and its metabolin in hair, has many advantages, such as that detection limit is low and high sensitivity.Also, by the certain concentration of each specific components, the dosage of protease can be greatly reduced, on the basis of guaranteeing detection efficiency, effectively save cost.
Description
Technical field
The present invention relates to rapid detection technical field, more particularly, to a kind of hair extracting solution, preparation method and application with
And illicit drugs inspection method.
Background technique
Drug abuse has become one of the Social Events that the current whole world needs to face, and drug abuse is (especially
Drugs) social security is seriously threatened, decline national society's productivity, carrys out serious influence to national society economy-zone.
Common drug abuse in-situ check and test method is immunoassay.Immune chromatography test paper Fast Detection Technique is in Dan Ke
A kind of Novel free epidemiology to grow up on the basis of grand antibody technique, immunochromatography technique, new material and labelling technique is quickly examined
Survey technology is the principle based on antigen and antibody specific association reaction, to detect various substances such as drug, hormone, protein.
For drugs consumer, the illicit drugs inspection in saliva, urine is the essential a part of illicit drugs inspection work.It grinds
Study carefully show personnel suck or shoot up after, drug ingredient can remain within a very long time human body saliva, urine
In liquid.Therefore, by the drugs content in inspection saliva, urine, the drug abuse situation of suspect can quickly be screened.Traditional urine
The shortcomings that biological fluids such as liquid, blood, saliva: the detection time limit is short (being only capable of embodying the kif of organism in a short time),
It is not easy to the disadvantages of acquiring and repeating sampling (being related to individual privacy etc.).
In view of this, the present invention is specifically proposed.
Summary of the invention
The first purpose of this invention is to provide a kind of hair extracting solution, at least to alleviate skill existing in the prior art
One of art problem.
Second object of the present invention is to provide the preparation method of above-mentioned hair extracting solution, this method simple process, behaviour
The hair extracting solution for facilitating, and being prepared can effectively detect the residual of the drugs in hair.
Third object of the present invention is to provide application of the above-mentioned hair extracting solution in illicit drugs inspection.
Fourth object of the present invention is to provide a kind of illicit drugs inspection method, this method cooperation hair provided by the invention
Extracting solution can be effectively reduced drugs detection limit, while the sensitivity of effectively enhancing illicit drugs inspection.
The present invention provides a kind of hair extracting solution, the hair extracting solution mainly consist of the following components: 100-200mM
The Qula that SDS that NaCl, 0.1-2mM CaCl, mass concentration are 0.1%-1%, volumetric concentration are 0.01%-0.1% leads to X-
100 and 50-200 μ g/ml Proteinase K, solvent is PBS solution.
Further, the hair extracting solution mainly consist of the following components: 120-180mM NaCl, 0.5-1.5mM
The triton x-100 and 60- that SDS that CaCl, mass concentration are 0.2%-0.8%, volumetric concentration are 0.02%-0.08%
150 μ g/ml Proteinase Ks, solvent is PBS solution.
Further, the hair extracting solution mainly consist of the following components: 150mM NaCl, 1mM CaCl, quality are dense
Spend the SDS for 0.5%, the triton x-100 and 100 μ g/ml Proteinase Ks that volumetric concentration is 0.05%, solvent is that PBS is molten
Liquid.
The present invention also provides the preparation methods of above-mentioned hair extracting solution, by NaCl, CaCl, SDS, triton x-100
And Proteinase K is added in PBS solution and is uniformly mixed, and obtains the hair extracting solution of formula concentration.
The present invention also provides above-mentioned hair extracting solution or the hair extractions being prepared using above-mentioned preparation method
Application of the liquid in illicit drugs inspection.
In addition, pretreated sample to be tested hair is added above-mentioned the present invention also provides a kind of illicit drugs inspection method
Hair extracting solution or the hair extracting solution that is prepared using above-mentioned preparation method in, detected after reaction.
Further, the pretreatment includes impregnating sample to be tested hair jointly with glyceryl monooleate.
Further, the pretreatment further includes crushing the sample to be tested hair for impregnating glyceryl monooleate
Step.
Further, sample to be tested hair is added in hair extracting solution, carries out ultrasound and magnetic field reaction, is then examined
It surveys；
Preferably, the power of the ultrasound is 450-550W；
Preferably, the magnetic induction intensity in the magnetic field is 200-300mT；
Preferably, described to be detected as dry type fluorescence detection.
Further, further include the steps that cleaning the sample to be tested hair before pretreatment；
Preferably, the sample to be tested hair is cleaned using organic solvent；
Preferably, alternately cleaning is carried out to the sample to be tested hair using organic solution and water.
Hair extracting solution provided by the invention mainly consist of the following components: 100-200mM NaCl, 0.1-2mM CaCl,
The triton x-100 and 50-200 μ g/ml egg that SDS that mass concentration is 0.1%-1%, volumetric concentration are 0.01%-0.1%
White enzyme K, solvent are PBS solution.Hair extracting solution provided by the invention is cooperated by each specific components of certain concentration, energy
Enough drugs and its metabolin effectively detected in hair have many advantages, such as that detection limit is low and high sensitivity.Also, pass through each spy
Determine the certain concentration of component, the dosage of protease can be greatly reduced, on the basis of guaranteeing detection efficiency, it is effectively save at
This.
The preparation method of hair extracting solution provided by the invention, by NaCl, CaCl, SDS, triton x-100 and albumen
Enzyme K is added in PBS solution and is uniformly mixed, and obtains the hair extracting solution of formula concentration.It is this method simple process, easy to operate,
And the hair extracting solution being prepared can effectively detect the residual of the drugs in hair.
Hair provided by the invention is added in pretreated sample to be tested hair by illicit drugs inspection method provided by the invention
It in extracting solution, is detected after reaction, this method cooperates hair extracting solution provided by the invention, can be effectively reduced drugs detection
Limit, while the sensitivity of effectively enhancing illicit drugs inspection.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
It should be understood that
In the present invention, if without particularly illustrating, all embodiments mentioned in this article and preferred implementation method
It can be combined with each other to form new technical solution.
In the present invention, if without particularly illustrating, all technical characteristics and preferred feature mentioned in this article can be with
Intercombination forms new technical solution.
In the present invention, if percentage (%) or part refer to the weight relative to composition without particularly illustrating
Percentage or parts by weight.
In the present invention, if related each component or its preferred ingredient can be combined with each other shape without particularly illustrating
The technical solution of Cheng Xin.
In the present invention, unless otherwise indicated, numberical range " a~b " indicates the contracting of any real combinings between a to b
Sketch form shows that wherein a and b is real number.Such as numberical range " 3~30 " indicate herein all listed " 3~30 " it
Between whole real numbers, " 3~30 " be these combinations of values breviary indicate.
" range " disclosed in this invention can be respectively one or more lower limits and one in the form of lower and upper limit
A or multiple upper limits.
In the present invention, unless otherwise indicated, it is each reaction or operating procedure can sequentially carry out, can also in sequence into
Row.Preferably, reaction method herein is that sequence carries out.
Unless otherwise indicated, profession used herein and meaning phase known to scientific term and one skilled in the art
Together.In addition, any method similar to or equal to what is recorded or material can also be applied in the present invention.
The present invention provides a kind of hair extracting solution, the hair extracting solution mainly consist of the following components: 100-200mM
The Qula that SDS that NaCl, 0.1-2mM CaCl, mass concentration are 0.1%-1%, volumetric concentration are 0.01%-0.1% leads to X-
100 and 50-200 μ g/ml Proteinase K, solvent is PBS solution.
Wherein, NaCl for example can be, but be not limited to 100mM, 110mM, 120mM, 130mM, 140mM, 150mM,
160mM, 170mM, 180mM, 190mM or 200mM；CaCl for example can be, but be not limited to 0.1mM, 0.2mM, 0.5mM,
0.8mM, 1mM, 1.2mM, 1.5mM, 1.8mM or 2mM；The mass concentration of SDS for example can be, but be not limited to 0.1%, 0.2%,
0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1%；The volumetric concentration of triton x-100 for example can be with
For, but be not limited to 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09% or
0.1%；Proteinase K for example can be, but be not limited to 50 μ g/ml, 80 μ g/ml, 100 μ g/ml, 120 μ g/ml, 150 μ g/ml,
180 μ g/ml or 200 μ g/ml.It should be noted that the concentration of each component is its end in hair extracting solution in this hair
Concentration.Solvent PBS is phosphate buffered saline solution, and in the present invention, solvent PBS can be common commercially available, or real
Room is tested voluntarily to configure.
Hair extracting solution provided by the invention is cooperated by each specific components of certain concentration, can effectively be detected
Drugs and its metabolin in hair have many advantages, such as that detection limit is low and high sensitivity.Also, pass through the specific of each specific components
Concentration can greatly reduce the dosage of protease, on the basis of guaranteeing detection efficiency, effectively save cost.Meanwhile hair is made
For a kind of detection material taken drugs and assert, have than the analysis of the biological fluids such as traditional urine, blood, saliva apparent superior
Property, such as limit for length when hair has detection, it is easy to acquire and repeat to sample, can be with long-term preservation the features such as.
In some preferred embodiments, the hair extracting solution mainly consist of the following components: 120-180mM
The Qula that SDS that NaCl, 0.5-1.5mM CaCl, mass concentration are 0.2%-0.8%, volumetric concentration are 0.02%-0.08%
Logical X-100 and 60-150 μ g/ml Proteinase K, solvent is PBS solution.
In some preferred embodiments, the hair extracting solution mainly consist of the following components: 150mM NaCl,
The triton x-100 and 100 μ g/ml protease that SDS that 1mM CaCl, mass concentration are 0.5%, volumetric concentration are 0.05%
K, solvent are PBS solution.
By further limiting to each component concentration in hair extracting solution, hair provided by the invention is enabled to extract
Liquid can more effectively extract drugs and metabolite in hair, have higher recall rate and higher sensitivity.
The present invention also provides the preparation methods of above-mentioned hair extracting solution, by NaCl, CaCl, SDS, triton x-100
And Proteinase K is added in PBS solution and is uniformly mixed, and obtains the hair extracting solution of formula concentration.
It is this method simple process, easy to operate, and the hair extracting solution being prepared can effectively detect the poison in hair
Product residual.
It should be noted that not limiting when preparing hair extracting solution provided by the invention the addition sequence of each component
It is fixed, as long as the concentration of final each component is within the scope of restriction of the invention.Preferably, be eventually adding Proteinase K, finally plus
The activity of enzyme can more effectively be guaranteed by entering Proteinase K, guarantee detection efficiency and high sensitivity.
The present invention also provides above-mentioned hair extracting solution or the hair extractions being prepared using above-mentioned preparation method
Application of the liquid in illicit drugs inspection.
In addition, pretreated sample to be tested hair is added above-mentioned the present invention also provides a kind of illicit drugs inspection method
Hair extracting solution or the hair extracting solution that is prepared using above-mentioned preparation method in, detected after reaction.
This method cooperates hair extracting solution provided by the invention, can be effectively reduced drugs detection limit, while effectively enhancing
The sensitivity of illicit drugs inspection.
In the present invention, sample to be tested hair can be hair, fine hair, armpit hair, vibrissa or eyebrow etc..
In some preferred embodiments, the pretreatment includes that sample to be tested hair and glyceryl monooleate is common
It impregnates.
By impregnating sample to be tested hair and glyceryl monooleate jointly, it can effectively soften hair tissue, more favorably
In post-processing, and then improve detection efficiency.
In some preferred embodiments, the pretreatment further includes the sample to be tested that will impregnate glyceryl monooleate
The step of hair is crushed.
By the processing crushed to hair sample, the substance in hair sample can be further set sufficiently to discharge molten
Out, detection limit can be further decreased, the sensitivity of detection is improved.
Wherein, without limitation to the mode of crushing, pulverizer can be used to be crushed, mortar also can be used and ground
Mill.Preferably, it is crushed in an inert atmosphere, carry out crushing in an inert atmosphere to guarantee in sample to be tested as far as possible
Contained substance is not influenced by oxidation, improves the accuracy of detection.
In some preferred embodiments, sample to be tested hair is added in hair extracting solution, carries out ultrasound and magnetic field
Reaction, is then detected.By ultrasound and magnetic field reaction cooperation combination, can make sample to be tested in hair extracting solution more
Adequately dissolution, and each substance in sample to be tested hair is promoted to dissolve out, reach reduction detection limit, improves the sensitivity of detection
Effect.
Preferably, the power of the ultrasound is 450-550W, such as can be, but be not limited to 450W, 460W, 470W,
480W, 490W, 500W, 510W, 520W, 530W, 540W or 550W.
Preferably, the magnetic induction intensity in the magnetic field is 200-300mT, such as can be, but be not limited to 200mT,
210mT, 220mT, 230mT, 240mT, 250mT, 260mT, 270mT, 280mT, 290mT or 300mT.
When sample to be tested hair under the action of the ultrasonic power of above-mentioned restriction and magnetic induction intensity with hair extracting solution into
When row reaction, recall rate is lower, and sensitivity is higher.
Preferably, described to be detected as dry type fluorescence detection.
In some preferred embodiments, further include the steps that cleaning the sample to be tested hair before pretreatment.It is logical
It crosses and sample to be tested is cleaned before detection, unnecessary impurity can be removed, improve the efficiency and accuracy of detection.
Preferably, the sample to be tested hair is cleaned using organic solvent.
Preferably organic solvent includes low-carbon alcohols, and typical low-carbon alcohols can be methanol, ethyl alcohol or propyl alcohol, preferably second
Alcohol.Ethyl alcohol is from a wealth of sources, and does not have toxic side effect, and have the advantages that low in cost.
Preferably, alternately cleaning is carried out to the sample to be tested hair using organic solution and water.It is understood that handing over
It for example can be first to be cleaned using organic solvent to sample to be tested hair for cleaning, after removing organic solvent, use water pair
Sample to be tested hair is cleaned, and after going water removal, is reused organic solvent and is cleaned to sample to be tested hair.Using organic molten
Liquid and water carry out the mode of alternately cleaning to the sample to be tested hair, and can more thoroughly remove need not in sample to be tested hair
The impurity wanted further increases the efficiency and accuracy of detection.
It elaborates combined with specific embodiments below with comparative example to the present invention.
Embodiment 1
Present embodiments provide a kind of hair extracting solution, hair extracting solution mainly consist of the following components:
The triton x-100 that SDS that 100mM NaCl, 2mM CaCl, mass concentration are 0.1%, volumetric concentration are 0.1%
And 50 μ g/ml Proteinase Ks, solvent is PBS solution.
Embodiment 2
Present embodiments provide a kind of hair extracting solution, hair extracting solution mainly consist of the following components:
The Qula that SDS that 200mM NaCl, 0.1mM CaCl, mass concentration are 1%, volumetric concentration are 0.01% leads to X-
100 and 200 μ g/ml Proteinase Ks, solvent is PBS solution.
Embodiment 3
Present embodiments provide a kind of hair extracting solution, hair extracting solution mainly consist of the following components:
The Qula that SDS that 120mM NaCl, 1.5mM CaCl, mass concentration are 0.2%, volumetric concentration are 0.08% leads to X-
100 and 60 μ g/ml Proteinase Ks, solvent is PBS solution.
Embodiment 4
Present embodiments provide a kind of hair extracting solution, hair extracting solution mainly consist of the following components:
The Qula that SDS that 180mM NaCl, 0.5mM CaCl, mass concentration are 0.8%, volumetric concentration are 0.02% leads to X-
100 and 150 μ g/ml Proteinase Ks, solvent is PBS solution.
Embodiment 5
Present embodiments provide a kind of hair extracting solution, hair extracting solution mainly consist of the following components:
The Qula that SDS that 150mM NaCl, 1mM CaCl, mass concentration are 0.5%, volumetric concentration are 0.05% leads to X-
100 and 100 μ g/ml Proteinase Ks, solvent is PBS solution.
Comparative example 1
This comparative example provides a kind of hair extracting solution, hair extracting solution mainly consist of the following components:
The Qula that SDS that 80mM NaCl, 2.4mM CaCl, mass concentration are 0.08%, volumetric concentration are 0.12% leads to X-
100 and 30 μ g/ml Proteinase Ks, solvent is PBS solution.
Comparative example 2
This comparative example provides a kind of hair extracting solution, hair extracting solution mainly consist of the following components:
The triton x-100 and 100 μ that SDS that 150mM NaCl, mass concentration are 0.5%, volumetric concentration are 0.05%
G/ml Proteinase K, solvent are PBS solution.
Comparative example 3
This comparative example provides a kind of hair extracting solution, hair extracting solution mainly consist of the following components:
150mM NaCl, 1mM CaCl, the SDS and 100 μ g/ml Proteinase Ks that mass concentration is 0.5%, solvent PBS
Solution.
Embodiment 6-10
A kind of illicit drugs inspection method is present embodiments provided, is included the following steps:
(a) sample to be tested hair is successively passed through ethyl alcohol, water, ethyl alcohol to clean, is dried after cleaning at 37 DEG C；
(b) sample to be tested hair and glyceryl monooleate that step (a) obtains are impregnated into 10min jointly；
(c) the sample to be tested hair that step (b) obtains is subjected to pulverization process after 37 DEG C of drying under nitrogen atmosphere；
(d) the smashed hair of 5mg is weighed, is added in the hair extracting solution of embodiment 1-5 offer, it is then super in 500W
5min is reacted under sound and 250mT magnetic field condition.
(e) the hair extracting solution for obtaining step (d) is added dropwise in ketamine test strip and combines Guangzhou great Mo company
Dry type fluorescence detector tested.
Embodiment 11
A kind of illicit drugs inspection method is present embodiments provided, is included the following steps:
(a) sample to be tested hair is successively passed through ethyl alcohol, water, ethyl alcohol to clean, is dried after cleaning at 37 DEG C；
(b) sample to be tested hair and glyceryl monooleate that step (a) obtains are impregnated into 10min jointly；
(c) the sample to be tested hair that step (b) obtains is subjected to pulverization process after 37 DEG C of drying under nitrogen atmosphere；
(d) the smashed hair of 5mg is weighed, is added in the hair extracting solution of the offer of embodiment 5, then in 500W ultrasound
And 5min is reacted under 250mT magnetic field condition.
(e) the hair extracting solution for obtaining step (d) is added dropwise in crystal methamphetamine test strip and combines the big footpath between fields in Guangzhou
The dry type fluorescence detector of company is tested.
Embodiment 12
A kind of illicit drugs inspection method is present embodiments provided, is included the following steps:
(a) sample to be tested hair is successively passed through ethyl alcohol, water, ethyl alcohol to clean, is dried after cleaning at 37 DEG C；
(b) sample to be tested hair and glyceryl monooleate that step (a) obtains are impregnated into 10min jointly；
(c) the sample to be tested hair that step (b) obtains is subjected to pulverization process after 37 DEG C of drying under nitrogen atmosphere；
(d) the smashed hair of 5mg is weighed, is added in the hair extracting solution of the offer of embodiment 5, then in 500W ultrasound
And 5min is reacted under 250mT magnetic field condition.
(e) the hair extracting solution for obtaining step (d) is added dropwise in morphine test strip and combines Guangzhou great Mo company
Dry type fluorescence detector is tested.
Embodiment 13
A kind of illicit drugs inspection method is present embodiments provided, is included the following steps:
(a) sample to be tested hair is cleaned by ethyl alcohol, is dried after cleaning at 37 DEG C；
(b) the sample to be tested hair that step (a) obtains is subjected to pulverization process after 37 DEG C of drying；
(c) the smashed hair of 5mg is weighed, is added in the hair extracting solution of the offer of embodiment 5,5min is reacted.
(d) the hair extracting solution for obtaining step (c) is added dropwise in ketamine test strip and combines Guangzhou great Mo company
Dry type fluorescence detector tested.
Embodiment 14
A kind of illicit drugs inspection method is present embodiments provided, is included the following steps:
(a) sample to be tested hair is successively passed through ethyl alcohol, water, ethyl alcohol to clean, is dried after cleaning at 37 DEG C；
(b) sample to be tested hair and glyceryl monooleate that step (a) obtains are impregnated into 10min jointly；
(c) the sample to be tested hair that step (b) obtains is subjected to pulverization process after 37 DEG C of drying under nitrogen atmosphere；
(d) the smashed hair of 5mg is weighed, is added in the hair extracting solution of embodiment 1-5 offer, it is then super in 300W
5min is reacted under sound and 400mT magnetic field condition.
(e) the hair extracting solution for obtaining step (d) is added dropwise in ketamine test strip and combines Guangzhou great Mo company
Dry type fluorescence detector tested.
Comparative example 4-6
This comparative example provides a kind of illicit drugs inspection method, includes the following steps:
(a) sample to be tested hair is successively passed through ethyl alcohol, water, ethyl alcohol to clean, is dried after cleaning at 37 DEG C；
(b) sample to be tested hair and glyceryl monooleate that step (a) obtains are impregnated into 10min jointly；
(c) the sample to be tested hair that step (b) obtains is subjected to pulverization process after 37 DEG C of drying under nitrogen atmosphere；
(d) the smashed hair of 5mg is weighed, is added in the hair extracting solution of comparative example 1-3 offer, it is then super in 500W
5min is reacted under sound and 250mT magnetic field condition.
(e) the hair extracting solution for obtaining step (d) is added dropwise in ketamine test strip and combines Guangzhou great Mo company
Dry type fluorescence detector tested.
Above-mentioned testing result is as shown in the table:
As can be seen that the hair extracting solution that 1-5 of the embodiment of the present invention is provided, passes through each of certain concentration in from the above
Specific components cooperate, and can effectively detect the drugs and its metabolin in hair, have detection limit low and high sensitivity
The advantages that.And the hair extracting solution that comparative example 1-3 is provided has not been able to detect the drugs in sample.
Wherein, embodiment 6-10 applies the hair extracting solution of 1-5 of embodiment of the present invention offer to sample to be tested hair respectively
Hair is detected, its raw material components of the hair extracting solution of embodiment 1-5 offer are all the same, and only the content of each component is different.But it is real
The hair extracting solution for applying the offer of example 5 is better than embodiment 1-4, the hair extracting solution that embodiment 2 and 3 provides to the verification and measurement ratio of drugs
Examples 1 and 2 are better than to the verification and measurement ratio of drugs.Illustrate further to be adjusted and optimized by the content to each component,
So that hair extracting solution provided by the invention has lower detection limit and higher sensitivity.
The hair extracting solution that embodiment 11 and embodiment 12 are provided using the embodiment of the present invention 5 respectively is to sample to be tested hair
In crystal methamphetamine and morphine detected, obtain the content of crystal methamphetamine and morphine in hair, offer of the present invention be provided
Hair extracting solution detection effect is all had to a variety of drugs.
Embodiment 10 and embodiment 13 and 14 are the hair extracting solutions using the offer of the embodiment of the present invention 5 to sample to be tested
Hair is detected, but illicit drugs inspection method used in embodiment 13 does not clean alternately, impregnates glyceryl monooleate and ultrasound
With the preferred steps such as magnetic field, in illicit drugs inspection method used in embodiment 14, the magnetic induction intensity of ultrasonic power and magnetic field is not
In preferred scope of the invention, the verification and measurement ratio of embodiment 10 is higher than embodiment 13 and 14, illustrates to extract in hair used
Liquid has lower detection limit and higher sensitivity under the same conditions, using preferred detection method provided by the invention.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. a kind of hair extracting solution, which is characterized in that the hair extracting solution mainly consist of the following components: 100-200mM
The Qula that SDS that NaCl, 0.1-2mM CaCl, mass concentration are 0.1%-1%, volumetric concentration are 0.01%-0.1% leads to X-
100 and 50-200 μ g/ml Proteinase K, solvent is PBS solution.
2. hair extracting solution according to claim 1, which is characterized in that the hair extracting solution is mainly by following components group
At: SDS, the volumetric concentration 0.02%- that 120-180mM NaCl, 0.5-1.5mM CaCl, mass concentration are 0.2%-0.8%
0.08% triton x-100 and 60-150 μ g/ml Proteinase K, solvent is PBS solution.
3. hair extracting solution according to claim 1, which is characterized in that the hair extracting solution is mainly by following components group
At: triton x-100 that SDS that 150mM NaCl, 1mM CaCl, mass concentration are 0.5%, volumetric concentration are 0.05% and
100 μ g/ml Proteinase Ks, solvent is PBS solution.
4. the preparation method of hair extracting solution as described in any one of claims 1-3, which is characterized in that by NaCl, CaCl,
SDS, triton x-100 and Proteinase K are added in PBS solution and are uniformly mixed, and obtain the hair extracting solution of formula concentration.
5. hair extracting solution as described in any one of claims 1-3 is prepared into using preparation method as claimed in claim 4
To application of the hair extracting solution in illicit drugs inspection.
6. a kind of illicit drugs inspection method, which is characterized in that it is any that claim 1-3 is added in pretreated sample to be tested hair
In hair extracting solution described in item or the hair extracting solution for applying preparation method as claimed in claim 4 to be prepared, after reaction
It is detected.
7. illicit drugs inspection method according to claim 6, which is characterized in that the pretreatment includes by sample to be tested hair
It is impregnated jointly with glyceryl monooleate.
8. illicit drugs inspection method according to claim 7, which is characterized in that the pretreatment further includes that will impregnate single oil
The step of sample to be tested hair of acid glyceride is crushed.
9. illicit drugs inspection method according to claim 6, which is characterized in that hair extracting solution is added in sample to be tested hair
In, ultrasound and magnetic field reaction are carried out, is then detected；
Preferably, the power of the ultrasound is 450-550W；
Preferably, the magnetic induction intensity in the magnetic field is 200-300mT；
Preferably, described to be detected as dry type fluorescence detection.
10. according to the described in any item illicit drugs inspection methods of claim 6-9, which is characterized in that before pretreatment further include clear
The step of washing the sample to be tested hair；
Preferably, the sample to be tested hair is cleaned using organic solvent；
Preferably, alternately cleaning is carried out to the sample to be tested hair using organic solution and water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910603285.0A CN110231323A (en) | 2019-07-04 | 2019-07-04 | Hair extracting solution, preparation method and application and illicit drugs inspection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910603285.0A CN110231323A (en) | 2019-07-04 | 2019-07-04 | Hair extracting solution, preparation method and application and illicit drugs inspection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110231323A true CN110231323A (en) | 2019-09-13 |
Family
ID=67856787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910603285.0A Pending CN110231323A (en) | 2019-07-04 | 2019-07-04 | Hair extracting solution, preparation method and application and illicit drugs inspection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110231323A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110987548A (en) * | 2019-10-11 | 2020-04-10 | 广州万孚生物技术股份有限公司 | Method for detecting hair poison, pretreatment reagent and kit |
| CN111983222A (en) * | 2020-07-08 | 2020-11-24 | 北京金鹏达科技发展有限公司 | Drug extracting solution in hair, extracting method and application |
| CN112986567A (en) * | 2021-01-27 | 2021-06-18 | 佛山微侦警用科技有限公司 | Reagent component ratio and preparation method of multi-project rapid quantitative drug detection box |
| CN115060562A (en) * | 2022-06-27 | 2022-09-16 | 上海凯创生物技术有限公司 | Hair drug trace detection extracting solution and preparation and use methods thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0458594A2 (en) * | 1990-05-22 | 1991-11-27 | Psychemedics Corporation | Analysis of hair |
| US5466579A (en) * | 1987-12-28 | 1995-11-14 | Psychemedics Corporation | Hair analysis method |
| CN101508716A (en) * | 2009-03-24 | 2009-08-19 | 中南大学 | Hair stem cracking liquid |
| CN104422607A (en) * | 2013-09-05 | 2015-03-18 | 杭州优玛达生物科技有限公司 | Hair pretreatment method |
| CN105767800A (en) * | 2016-03-11 | 2016-07-20 | 广西至臻投资有限公司 | Siraitia grosvenorii drink |
| CN108844922A (en) * | 2018-05-17 | 2018-11-20 | 浙江诺迦生物科技有限公司 | The rapid detection method of drugs in a kind of hair |
| CN109349571A (en) * | 2018-11-26 | 2019-02-19 | 徐丽琴 | The preparation method of arbutus jam and its arbutus jam obtained |
| CN109613230A (en) * | 2018-12-29 | 2019-04-12 | 上海八通生物科技股份有限公司 | The fast quantitative measurement method for detecting of drugs in a kind of hair |
| WO2019071495A1 (en) * | 2017-10-11 | 2019-04-18 | 广东南天司法鉴定所 | Method for screening psychotropic drugs from hair |
-
2019
- 2019-07-04 CN CN201910603285.0A patent/CN110231323A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5466579A (en) * | 1987-12-28 | 1995-11-14 | Psychemedics Corporation | Hair analysis method |
| EP0458594A2 (en) * | 1990-05-22 | 1991-11-27 | Psychemedics Corporation | Analysis of hair |
| CN101508716A (en) * | 2009-03-24 | 2009-08-19 | 中南大学 | Hair stem cracking liquid |
| CN104422607A (en) * | 2013-09-05 | 2015-03-18 | 杭州优玛达生物科技有限公司 | Hair pretreatment method |
| CN105767800A (en) * | 2016-03-11 | 2016-07-20 | 广西至臻投资有限公司 | Siraitia grosvenorii drink |
| WO2019071495A1 (en) * | 2017-10-11 | 2019-04-18 | 广东南天司法鉴定所 | Method for screening psychotropic drugs from hair |
| CN108844922A (en) * | 2018-05-17 | 2018-11-20 | 浙江诺迦生物科技有限公司 | The rapid detection method of drugs in a kind of hair |
| CN109349571A (en) * | 2018-11-26 | 2019-02-19 | 徐丽琴 | The preparation method of arbutus jam and its arbutus jam obtained |
| CN109613230A (en) * | 2018-12-29 | 2019-04-12 | 上海八通生物科技股份有限公司 | The fast quantitative measurement method for detecting of drugs in a kind of hair |
Non-Patent Citations (1)
| Title |
|---|
| 刘俊亭 等: "毛发中酪胺气相色谱-质谱联用仪测定方法的研究", 《中国药物滥用防治杂志》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110987548A (en) * | 2019-10-11 | 2020-04-10 | 广州万孚生物技术股份有限公司 | Method for detecting hair poison, pretreatment reagent and kit |
| WO2021068417A1 (en) * | 2019-10-11 | 2021-04-15 | 广州万孚生物技术股份有限公司 | Method, pretreatment reagent, and kit for detecting drug in hair |
| CN111983222A (en) * | 2020-07-08 | 2020-11-24 | 北京金鹏达科技发展有限公司 | Drug extracting solution in hair, extracting method and application |
| CN112986567A (en) * | 2021-01-27 | 2021-06-18 | 佛山微侦警用科技有限公司 | Reagent component ratio and preparation method of multi-project rapid quantitative drug detection box |
| CN115060562A (en) * | 2022-06-27 | 2022-09-16 | 上海凯创生物技术有限公司 | Hair drug trace detection extracting solution and preparation and use methods thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110231323A (en) | Hair extracting solution, preparation method and application and illicit drugs inspection method | |
| Lee et al. | SERS analysis of selectively captured exosomes using an integrin‐specific peptide ligand | |
| Zhu et al. | Effects of extracts from sporoderm-broken spores of Ganoderma lucidum on HeLa cells | |
| O'GRODNICK | Whirling disease Myxosoma cerebralis spore concentration using the continuous plankton centrifuge | |
| Hansen et al. | An extended database of keratin binding | |
| Viviani et al. | Treatment and serological studies of an Italian case of penicilliosis marneffei contracted in Thailand by a drug addict infected with the human immunodeficiency virus | |
| CN112961899A (en) | Method for screening anti-inflammatory efficacy of in-vitro macrophage combined 3D skin model of cosmetic raw material | |
| CN1201148C (en) | Process for quality control and standardisation of medicinal plant products | |
| CN107917970A (en) | The method of examination psychotropic agent from hair | |
| CN109283274A (en) | A kind of multicomponent content assaying method of Hedan tablet | |
| CN112946111B (en) | Method for constructing and identifying UPLC fingerprint of rhizoma Drynariae crude product and its processed product | |
| Santos et al. | Improved radiocarbon analyses of modern human hair to determine the year‐of‐death by cross‐flow nanofiltered amino acids: common contaminants, implications for isotopic analysis, and recommendations | |
| CN102520154B (en) | Method for detecting dioxins in environment | |
| CN101852787A (en) | Method for screening active ingredients of Chinese medicament | |
| Giovannetti et al. | Magnetic resonance spectroscopy and imaging for the study of fossils | |
| CN109045311B (en) | Prussian blue nano MRI tracer agent and preparation method and application thereof | |
| CN108663440A (en) | Callicarpa nudiflora medicinal material UPLC fingerprint map constructions method and standard finger-print | |
| CN106932523B (en) | The fingerprint atlas detection method and method of quality control of oriental wormwood dispensing granule | |
| CN115267008A (en) | Method for constructing characteristic spectrum and contrast spectrum of pinellia ternata (thunb.) makino and method for distinguishing different processed products of pinellia ternata (thunb.) makino | |
| CN104398418A (en) | Hair-loss-resistant hair conditioner | |
| Nguyen et al. | Review of dragon millipedes (Diplopoda, Polydesmida, Paradoxosomatidae) in the fauna of Vietnam, with descriptions of three new species | |
| CN105699581A (en) | Construction method of UPLC fingerprint of sweet clover medicinal material and standard fingerprint thereof | |
| CN113970611B (en) | Endothelium corneum gigeriae galli specific polypeptide and application thereof in identification of endothelium corneum gigeriae galli | |
| de Paulo et al. | Crude extract of Fusarium oxysporum induces apoptosis and structural alterations in the skin of healthy rats | |
| CN106990182A (en) | The characteristic spectrum of Inula britannica chinensis granule is set up and detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190913 |