CN110218633A - A kind of system and method for continuous production bacteria cellulose film - Google Patents
A kind of system and method for continuous production bacteria cellulose film Download PDFInfo
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- CN110218633A CN110218633A CN201910567691.6A CN201910567691A CN110218633A CN 110218633 A CN110218633 A CN 110218633A CN 201910567691 A CN201910567691 A CN 201910567691A CN 110218633 A CN110218633 A CN 110218633A
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- bacteria cellulose
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- 241000894006 Bacteria Species 0.000 title claims abstract description 146
- 229920002678 cellulose Polymers 0.000 title claims abstract description 135
- 239000001913 cellulose Substances 0.000 title claims abstract description 134
- 238000010924 continuous production Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims description 59
- 238000000855 fermentation Methods 0.000 claims abstract description 158
- 230000004151 fermentation Effects 0.000 claims abstract description 158
- 239000011148 porous material Substances 0.000 claims abstract description 87
- 238000004140 cleaning Methods 0.000 claims abstract description 81
- 239000007788 liquid Substances 0.000 claims abstract description 76
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 47
- 239000002699 waste material Substances 0.000 claims abstract description 33
- 230000008676 import Effects 0.000 claims abstract description 29
- 239000002609 medium Substances 0.000 claims abstract description 25
- 239000001963 growth medium Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000010992 reflux Methods 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims description 27
- 238000004659 sterilization and disinfection Methods 0.000 claims description 16
- 230000010355 oscillation Effects 0.000 claims description 14
- 238000001514 detection method Methods 0.000 claims description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 238000006386 neutralization reaction Methods 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 9
- 239000000835 fiber Substances 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 abstract description 29
- 238000004519 manufacturing process Methods 0.000 abstract description 18
- 239000000284 extract Substances 0.000 abstract description 5
- 235000010980 cellulose Nutrition 0.000 description 124
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 46
- 239000012528 membrane Substances 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000002054 inoculum Substances 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- 238000003912 environmental pollution Methods 0.000 description 5
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- 239000000463 material Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000011218 seed culture Methods 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 239000010963 304 stainless steel Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 229910000589 SAE 304 stainless steel Inorganic materials 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000004900 laundering Methods 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000008104 plant cellulose Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 229920002749 Bacterial cellulose Polymers 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 241001596500 Komagataeibacter rhaeticus Species 0.000 description 1
- 229920001410 Microfiber Polymers 0.000 description 1
- 244000179886 Moringa oleifera Species 0.000 description 1
- 235000011347 Moringa oleifera Nutrition 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
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- 241001148470 aerobic bacillus Species 0.000 description 1
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- 239000005016 bacterial cellulose Substances 0.000 description 1
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- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
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- 238000003306 harvesting Methods 0.000 description 1
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- 238000009434 installation Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
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- 239000012567 medical material Substances 0.000 description 1
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- 238000005086 pumping Methods 0.000 description 1
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- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/16—Sterilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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Abstract
The present invention provides a kind of systems of continuous production bacteria cellulose film, comprising: fermentation vat；Fermentation vat is equipped with medium feed pipeline, air intake conduit and cleaning solution return line；It can extend into the sieve pore external member inside fermentation vat；Sieve pore external member is the frame structure that bottom surface is evenly distributed with several sieve pores, and side is equipped with windowing close to bottom position；The fortune device for hoisting that the oscilaltion of sieve pore external member can be controlled and moved horizontally；The kitchen sink being connected by cleaning solution return line with fermentation vat；Cleaning solution return line is successively arranged peristaltic pump and the first valve along cleaning solution reflux direction；Kitchen sink is equipped with oscillator, cleaning solution bypass outlet and feed inlet, and can accommodate sieve pore external member；Feed inlet is connected with culture medium import, lye import, waste liquid outlet and sterilizing water inlet respectively by the two-way peristaltic pump of multiple tracks.The system can be improved production efficiency, and bacteria cellulose film easily extracts, is easy to wash after fermentation on the basis of realizing continuous production bacteria cellulose film.
Description
Technical field
The present invention relates to technical field of microbial fermentation, are to be related to a kind of continuous production bacteria cellulose more specifically
The system and method for film.
Background technique
Bacteria cellulose (Bacterial cellulose, abbreviation BC) is that the porosity that is synthesized by microbial fermentation is netted
Nano grade biological high molecular polymer is named as bacteria cellulose because it is synthesized by bacterium.BC is by unique filamentary fibers group
At, fibre diameter is between 0.01~0.10 μm, 2~3 orders of magnitude smaller than plant cellulose (10 μm), each filamentary fibers by
A certain number of ultra-microfibers form reticular structure, with plant cellulose main difference is that it does not contain hemicellulose, wood
Quality etc..As a kind of novel nano-material, bacteria cellulose has been applied to the every field such as weaving, medical material, food, existing
Have become international research hotspot.
The production of existing bacteria cellulose mainly uses tray disposably to ferment to produce, such as application No. is
201620504633.0, the Chinese patent of 201820393003.X and 201810361078.4 propose using tray come disposably
Fermentation obtains bacteria cellulose.But above-mentioned fermentation method once can only generate a bacterial fibers in a tray top fermentation
Plain film, and containing a large amount of bacterium and culture medium in the bacteria cellulose film obtained after fermenting, according to the current methods bacterium
Cellulose membrane will enter sterilizing, neutralize and the process flow of washing, and there are the utilization rate of strain is too low and fermentation liquid for this technique
Waste problem.
In this regard, researcher is improved the utilization rate of strain using quantity-produced technique, reduces the waste of fermentation liquid,
But that there are production efficiencys is low, bacteria cellulose after fermentation for the method for continuous production bacteria cellulose film in the prior art
The technical problems such as film extraction is difficult, the output subsequent washing procedure of bacteria cellulose film is cumbersome.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of system and method for continuous production bacteria cellulose film,
Realization is continuously fermented produce bacteria cellulose film on the basis of, make full use of fermentation medium and strain, can be improved production efficiency,
And bacteria cellulose film easily extracts, is easy to wash after fermentation.
The present invention provides a kind of systems of continuous production bacteria cellulose film, comprising:
Fermentation vat；The fermentation vat is equipped with medium feed pipeline, air intake conduit and cleaning solution return line；
It can extend into the sieve pore external member inside fermentation vat；The sieve pore external member is that bottom surface is evenly distributed with several sieve pores
Frame structure, side are equipped with windowing close to bottom position；
The fortune device for hoisting that the oscilaltion of sieve pore external member can be controlled and moved horizontally；
The kitchen sink being connected by cleaning solution return line with fermentation vat；The cleaning solution return line flows back along cleaning solution
Direction is successively arranged peristaltic pump and the first valve；The kitchen sink is equipped with oscillator, cleaning solution bypass outlet and feed inlet, and energy
Enough accommodate sieve pore external member；The feed inlet by the two-way peristaltic pump of multiple tracks respectively with culture medium import, lye import, waste liquid outlet
It is connected with sterilizing water inlet.
Preferably, the medium feed pipeline is successively arranged the second valve, circulating pump and third valve along feedstock direction；
The circulating pump is additionally provided with the circulation fluid return line communicated with fermentation vat.
Preferably, the circulation fluid return line is equipped with the 4th valve.
Preferably, the air intake conduit is equipped with the 5th valve.
Preferably, the diameter of sieve pore is 5mm~15mm, length and the setting windowing of windowing in the sieve pore external member
The equal length of side is highly 45mm~55mm.
The present invention also provides a kind of methods of continuous production bacteria cellulose film, described by adopting the above technical scheme to be
System, comprising the following steps:
A) fermentation liquid is mixed with air in the internal fermentation vat equipped with sieve pore external member, is fermented, in sieve pore external member
In obtain the bacteria cellulose film with a thickness of 2mm~4mm, take out after sieve pore external member stands, be transferred in kitchen sink, using oscillation
Device is washed, the bacteria cellulose film after respectively obtaining cleaning solution and washing；Wherein, the cleaning solution is back to fermentation vat
In；
B) bacteria cellulose film after washing that step a) is obtained successively is neutralized and is sterilized, cleaned, obtain bacterium
Cellulose membrane product；
Meanwhile the initial water level that aqua sterilisa or fermentation liquid make fermentation liquid be restored to step a) is filled into fermentation vat, then
It is packed into sieve pore external member in fermentation vat, repeats step a) and step b), continuous production bacteria cellulose film product.
Preferably, the temperature of fermentation described in step a) is 28 DEG C~32 DEG C, and dissolved oxygen amount is 1mg/L~3mg/L.
Preferably, the process of washing described in step a) uses fermentation medium or aqua sterilisa；The oscillation of the oscillator
Speed is 100rpm~200rpm, and duration of oscillation is 25min~35min.
Preferably, neutralization described in step b) and the process sterilized specifically:
Start the two-way peristaltic pump of multiple tracks, the lye that 3L~10L mass fraction is 0.5%~1.5% is pumped by lye import
To kitchen sink, starts oscillator, vibrated under the hunting speed of 100rpm~200rpm, neutralized and sterilized, in pH electrode
Under detection when pH value no longer changes, restarts the two-way peristaltic pump of multiple tracks, the waste liquid in kitchen sink is all arranged by waste liquid outlet
Out.
Preferably, the process of cleaning described in step b) specifically:
Start the two-way peristaltic pump of multiple tracks, 3L~10L aqua sterilisa be pumped into kitchen sink by aqua sterilisa import, starts oscillator,
25min~35min is vibrated under the hunting speed of 100rpm~200rpm, is cleaned, and the two-way peristaltic pump of multiple tracks is then started,
Waste liquid in kitchen sink is all discharged by waste liquid outlet；After being cleaned multiple times, when pH stable is 7 or so under pH electrode detection
When, obtain bacteria cellulose film product.
The present invention provides a kind of system and method for continuous production bacteria cellulose film, the system comprises: fermentation vat；
The fermentation vat is equipped with medium feed pipeline, air intake conduit and cleaning solution return line；It can extend into inside fermentation vat
Sieve pore external member；The sieve pore external member is the frame structure that bottom surface is evenly distributed with several sieve pores, and side is close to bottom position
Equipped with windowing；The fortune device for hoisting that the oscilaltion of sieve pore external member can be controlled and moved horizontally；Pass through cleaning solution return line and hair
The connected kitchen sink in ferment pond；The cleaning solution return line is successively arranged peristaltic pump and the first valve along cleaning solution reflux direction；
The kitchen sink is equipped with oscillator, cleaning solution bypass outlet and feed inlet, and can accommodate sieve pore external member；The feed inlet passes through
The two-way peristaltic pump of multiple tracks is connected with culture medium import, lye import, waste liquid outlet and sterilizing water inlet respectively.With prior art phase
Than the system and method for continuous production bacteria cellulose film provided by the invention continuously ferment in realization and produce bacteria cellulose
On the basis of film, production efficiency can be improved, and bacteria cellulose film easily extracts, is easy to wash after fermentation.
Meanwhile the system and method for continuous production bacteria cellulose film provided by the invention can be improved the utilization of fermentation liquid
Rate makes full use of the value of strain and fermentation liquid；And it can be by washing, neutralization and sterilization, cleaning process in the same apparatus
It completes, saves occupied space, manpower and the lye dosage of system, reduce environmental pollution, have both the feature of environmental protection and economy, be conducive to
It promotes and develops in this field.
In addition, by the mold of the good specific dimensions of fixed design in sieve pore external member, can also output predetermined size bacterium
Cellulose membrane has a extensive future.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the system of continuous production bacteria cellulose film provided in an embodiment of the present invention；
Fig. 2 is the structural schematic diagram of the system for the continuous production bacteria cellulose film that the embodiment of the present invention 1 provides.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical solution of the present invention is clearly and completely described, it is clear that institute
The embodiment of description is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair
The range of bright protection.
The present invention provides a kind of systems of continuous production bacteria cellulose film, comprising:
Fermentation vat；The fermentation vat is equipped with medium feed pipeline, air intake conduit and cleaning solution return line；
It can extend into the sieve pore external member inside fermentation vat；The sieve pore external member is that bottom surface is evenly distributed with several sieve pores
Frame structure, side are equipped with windowing close to bottom position；
The fortune device for hoisting that the oscilaltion of sieve pore external member can be controlled and moved horizontally；
The kitchen sink being connected by cleaning solution return line with fermentation vat；The cleaning solution return line flows back along cleaning solution
Direction is successively arranged peristaltic pump and the first valve；The kitchen sink is equipped with oscillator, cleaning solution bypass outlet and feed inlet, and energy
Enough accommodate sieve pore external member；The feed inlet by the two-way peristaltic pump of multiple tracks respectively with culture medium import, lye import, waste liquid outlet
It is connected with sterilizing water inlet.
Referring to Fig. 1, Fig. 1 is the structural representation of the system of continuous production bacteria cellulose film provided in an embodiment of the present invention
Figure；Wherein, 1 is fermentation vat, and 2 be medium feed pipeline, and 3 be air intake conduit, and 4 be cleaning solution return line, and 5 be sieve pore
External member, 6 be sieve pore, and 7 be windowing, and 8 be fortune device for hoisting, and 9 be kitchen sink, and 10 be peristaltic pump, and 11 be the first valve, and 12 be oscillation
Device, 13 be cleaning solution bypass outlet, and 14 be feed inlet, and 15 be the two-way peristaltic pump of multiple tracks, and 16 be culture medium import, 17 for lye into
Mouthful, 18 be waste liquid outlet, and 19 be sterilizing water inlet.
In the present invention, the fermentation vat (1) is for producing bacteria cellulose film, preferably intelligent fermentation vat, Neng Goutong
Parameters (including residual sugar amount, dissolved oxygen amount, temperature, pH value, DO value, OD value etc.) in Different electrodes real time monitoring fermentation vat are crossed,
The various nutritional ingredients (including carbon source and nitrogen source etc.) that intelligence addition needs, do not need large fermentation tank to provide a large amount of seed
Liquid.
In the present invention, the fermentation vat (1) is equipped with medium feed pipeline (2), air intake conduit (3) and cleaning solution
Return line (4).In the present invention, the medium feed pipeline (2) is used for by culture medium, including the seed culture fluid of strain
Fermentation vat (1) is delivered into, fermentation vat (1) side lower is preferably provided at；The medium feed pipeline (2) is along feedstock direction
It is successively arranged the second valve, circulating pump and third valve；The circulating pump is additionally provided with the circulation fluid reflux communicated with fermentation vat (1)
Pipeline, the circulation fluid return line is by the fermentation vat (1) top feed.In the present invention, the circulation fluid return line
Equipped with the 4th valve.
In the present invention, the air intake conduit (3) is used to air delivery entering fermentation vat (1), is preferably provided at
Fermentation vat (1) bottom；The air intake conduit (3) is equipped with the 5th valve.
In the present invention, the cleaning solution return line (4) is used to cleaning solution being back to fermentation vat (1), is preferably provided with
In fermentation vat (1) side surface upper part.
In the present invention, the sieve pore external member (5) is used for when bacteria cellulose film reaches certain thickness, by bacterial fibers
Plain film takes out from fermentation vat (1)；The sieve pore external member (5) can extend into inside fermentation vat.In the present invention, the sieve pore set
Part (5) is the frame structure that bottom surface is evenly distributed with several sieve pores (6), and side is equipped with windowing (7) close to bottom position.
In the present invention, the diameter of sieve pore (6) is preferably 5mm~15mm in the sieve pore external member (5), more preferably
10mm；Equal length of the length to open a window in the sieve pore external member (5) preferably with the side that the windowing is arranged, in fact it is highly preferred that be
45mm~55mm, more preferably 50mm.The present invention is on the one hand taking out bacterium from fermentation vat (1) using above-mentioned sieve pore external member (5)
When cellulose membrane, the Partial fermentation liquid and strain taken out of in film can be come back in fermentation vat (1), it is on the other hand also advantageous
Cleaning solution comes into full contact with bacteria cellulose film in subsequent laundering process.In addition, preferably fixation is set in the sieve pore external member (5)
Counted the mold (such as model of facial mask, eye mask) of specific dimensions, can also output predetermined size bacteria cellulose film, reduce the later period
The process additionally by die cut, such as facial mask, eye mask are needed, is had a extensive future.
In the present invention, the fortune device for hoisting (8) is for controlling (5) oscilaltion of sieve pore external member and moving horizontally.At this
In invention, the fortune device for hoisting (8) can configure 2 groups of sieve pore external members (5) simultaneously, and 2 groups of sieve pore external members (5) use in turn, Bu Huishou
The influence taken a long time to subsequent wash.
In the present invention, the kitchen sink (9) has both sides to act on: first is that being just transferred out from fermentation vat (1)
Bacteria cellulose film impregnated and washed, and the cleaning solution containing culture medium and strain after washing is back to fermentation vat
(1) continuous lower bacteria cellulose film of fermenting and producing is relayed；Second is that the bacteria cellulose film after washing is neutralized and sterilized,
So that the pH of bacteria cellulose film is adjusted to neutral and remaining bacterium is killed；Last cleaning is carried out with aqua sterilisa again.Therefore
The kitchen sink (9) can complete in the same apparatus washing, neutralization and sterilization, cleaning process, and the occupancy for saving system is empty
Between, manpower and lye dosage, reduce environmental pollution, have both the feature of environmental protection and economy.In the present invention, the kitchen sink (9) is excellent
Standby pH electrode is matched, for the pH value during monitor wash.
In the present invention, the kitchen sink (9) is connected by cleaning solution return line (4) with fermentation vat (1).In the present invention
In, the cleaning solution return line (4) is successively arranged peristaltic pump (10) and the first valve (11) along cleaning solution reflux direction.
In the present invention, the kitchen sink (9) is equipped with oscillator (12), cleaning solution bypass outlet (13) and feed inlet
(14), and sieve pore external member (5) can be accommodated.In the present invention, the oscillator (12) is located at the lower part of kitchen sink (9), to wash
It washs pond (9) and oscillation power is provided, kitchen sink (9) is made to realize cyclotron oscillation.
In the present invention, the cleaning solution bypass outlet (13) communicates with cleaning solution return line (4).
In the present invention, the feed inlet (14) by the two-way peristaltic pump of multiple tracks (15) respectively with culture medium import (16),
Lye import (17), waste liquid outlet (18) are connected with sterilizing water inlet (19).
The present invention also provides a kind of methods of continuous production bacteria cellulose film, described by adopting the above technical scheme to be
System, comprising the following steps:
A) fermentation liquid is mixed with air in the internal fermentation vat equipped with sieve pore external member, is fermented, in sieve pore external member
In obtain the bacteria cellulose film with a thickness of 2mm~4mm, take out after sieve pore external member stands, be transferred in kitchen sink, using oscillation
Device is washed, the bacteria cellulose film after respectively obtaining cleaning solution and washing；Wherein, the cleaning solution is back to fermentation vat
In；
B) bacteria cellulose film after washing that step a) is obtained successively is neutralized and is sterilized, cleaned, obtain bacterium
Cellulose membrane product；
Meanwhile the initial water level that aqua sterilisa or fermentation liquid make fermentation liquid be restored to step a) is filled into fermentation vat, then
It is packed into sieve pore external member in fermentation vat, repeats step a) and step b), continuous production bacteria cellulose film product.
The present invention system by adopting the above technical scheme, first by fermentation liquid internal equipped with sieve pore external member (5)
Fermentation vat mixes with air in (1), ferments, and obtains the bacteria cellulose with a thickness of 2mm~4mm in sieve pore external member (5)
Film is transferred in kitchen sink (9) after taking out sieve pore external member (5) standing, is washed using oscillator (12), respectively obtain and wash
Bacteria cellulose film after washing liquid and washing；Wherein, the cleaning solution is back in fermentation vat (1).
The present invention is not particularly limited the fermentation liquid, is using preparation method well known to those skilled in the art
It can；The inoculum concentration of strain is preferably 5%~10% based on volumn concentration in the fermentation liquid；In currently preferred implementation
It is 6% in example.
In the present invention, the temperature of the fermentation is preferably 28 DEG C~32 DEG C, more preferably 30 DEG C；The dissolved oxygen of the fermentation
Amount is preferably 1mg/L~3mg/L, more preferably 2mg/L.In fermentation process, the surface that fermentation liquid is contacted with air can generate one
Layer bacteria cellulose, with the growth of time, the thickness of bacteria cellulose film can be continuously increased；The production bacteria cellulose it is thin
Bacterium is oxygen consumption bacterium, therefore when bacteria cellulose film reaches certain thickness, the content of oxygen in fermentation liquid can be made to reduce, in addition
The consumption of nutriment eventually leads to the stopping of bacteria cellulose thickness growth.
After the present invention obtains with a thickness of the bacteria cellulose film of 2mm~4mm in sieve pore external member (5), sieve pore external member is taken out
(5), preferably 3mm.
In the present invention, the time of the standing is preferably 8min~12min, more preferably 10min；Purpose is to make bacterium
The fermentation liquid and strain that cellulose membrane is taken out of return to fermentation vat (1) by the sieve pore (6) of sieve pore external member (5) bottom surface as far as possible
In, realize making full use of for strain and fermentation liquid.
In the present invention, the process of the washing preferably uses fermentation medium or aqua sterilisa, more preferably fermented and cultured
Base；The hunting speed of the oscillator (12) is preferably 100rpm~200rpm, more preferably 150rpm；The oscillator (12)
Duration of oscillation be preferably 25min~35min, more preferably 30min.
In the present invention, the process of the washing is preferred specifically:
Start the two-way peristaltic pump of multiple tracks (15), 3L~10L fermentation is pumped by culture medium import (16)/sterilizing water inlet (19)
Culture medium/aqua sterilisa starts oscillator (12), and 25min~35min is vibrated under the hunting speed of 100rpm~200rpm, into
Row washing, is distributed to remaining fermentation liquid and strain in bacteria cellulose film in fermentation medium, obtains cleaning solution；Then it beats
It opens the first valve (11), starts peristaltic pump (10), the cleaning solution that original fermentation liquor and strain are had in kitchen sink (9) is all flowed back
To in fermentation vat (1)；
More preferably:
Start the two-way peristaltic pump of multiple tracks (15), 5L fermented and cultured is pumped by culture medium import (16)/sterilizing water inlet (19)
Base/aqua sterilisa starts oscillator (12), vibrates 30min under the hunting speed of 150rpm, washed, make bacteria cellulose
Remaining fermentation liquid and strain are distributed in fermentation medium in film, obtain cleaning solution；Then it opens the first valve (11), starts
Peristaltic pump (10) all flows back into the cleaning solution that original fermentation liquor and strain are had in kitchen sink (9) in fermentation vat (1).
After bacteria cellulose after being washed, during the present invention successively carries out the bacteria cellulose film after obtained washing
With with sterilization, cleaning, obtain bacteria cellulose film product.In the present invention, the process of the neutralization and sterilization is preferred specifically:
Start the two-way peristaltic pump of multiple tracks (15), by lye import (17) be pumped into 3L~10L mass fraction be 0.5%~
1.5% lye starts oscillator (12), vibrates under the hunting speed of 100rpm~200rpm, in progress to kitchen sink (9)
With and sterilization, under pH electrode detection when pH value no longer changes, restart the two-way peristaltic pump of multiple tracks (15), by kitchen sink (9)
In waste liquid all by waste liquid outlet (18) be discharged；
More preferably:
Start the two-way peristaltic pump of multiple tracks (15), by lye import (17) be pumped into 5L mass fraction be 1% NaOH solution extremely
Kitchen sink (9) starts oscillator (12), vibrates under the hunting speed of 150rpm, neutralized and sterilized, in pH electrode detection
Under when pH value no longer changes, restart the two-way peristaltic pump of multiple tracks (15), by the waste liquid in kitchen sink (9) all by waste liquid outlet
(18) it is discharged.
In the present invention, it can guarantee that the amount of the lye added will not be excessive among the above and with the process of sterilization, avoid wave
Take and reduces environmental pollution.
In the present invention, the process of the cleaning is preferred specifically:
Start the two-way peristaltic pump of multiple tracks (15), 3L~10L aqua sterilisa be pumped into kitchen sink (9) by sterilizing water inlet (19),
Start oscillator (12), 25min~35min is vibrated under the hunting speed of 100rpm~200rpm, is cleaned, is then started
Waste liquid in kitchen sink (9) is all discharged by waste liquid outlet (18) the two-way peristaltic pump of multiple tracks (15)；After being cleaned multiple times, in pH
Under electrode detection when pH stable is 7 or so, bacteria cellulose film product is obtained；
More preferably:
Start the two-way peristaltic pump of multiple tracks (15), 5L aqua sterilisa is pumped into kitchen sink (9), starting vibration by sterilizing water inlet (19)
Device (12) are swung, 30min is vibrated under the hunting speed of 150rpm, is cleaned, then start the two-way peristaltic pump of multiple tracks (15), it will
Waste liquid in kitchen sink (9) is all discharged by waste liquid outlet (18)；After being cleaned multiple times, when pH stable exists under pH electrode detection
When 7 or so, bacteria cellulose film product is obtained.
The present invention carry out first open bacteria cellulose film washing, in and and sterilization, cleaning while, in fermentation vat (1)
In fill into the initial water level that aqua sterilisa or fermentation liquid make fermentation liquid be restored to step a), then sieve pore is packed into fermentation vat (1)
External member (5), repetition step a) obtain second bacteria cellulose film and carry out washing and step b) neutralization and sterilize, clean, obtain
Bacteria cellulose film product realizes the continuous production of bacteria cellulose film product.
The system and method for continuous production bacteria cellulose film provided by the invention continuously ferment in realization and produce bacterium fibre
On the basis of tieing up plain film, production efficiency can be improved, and bacteria cellulose film easily extracts (by sieve pore external member after fermentation
Window skids off), it is easy to wash.Meanwhile the system and method for continuous production bacteria cellulose film provided by the invention can be improved hair
The utilization rate of zymotic fluid makes full use of value (production technology of bacteria cellulose film in the prior art, the training of strain and fermentation liquid
Nutrient solution and strain are disposable, more waste)；And it can be by washing, neutralization and sterilization, cleaning process in same device
Middle completion saves occupied space, manpower and the lye dosage of system, reduces environmental pollution, have both the feature of environmental protection and economy, favorably
In in this field popularization and development.
The present invention provides a kind of system and method for continuous production bacteria cellulose film, the system comprises: fermentation vat；
The fermentation vat is equipped with medium feed pipeline, air intake conduit and cleaning solution return line；It can extend into inside fermentation vat
Sieve pore external member；The sieve pore external member is the frame structure that bottom surface is evenly distributed with several sieve pores, and side is close to bottom position
Equipped with windowing；The fortune device for hoisting that the oscilaltion of sieve pore external member can be controlled and moved horizontally；Pass through cleaning solution return line and hair
The connected kitchen sink in ferment pond；The cleaning solution return line is successively arranged peristaltic pump and the first valve along cleaning solution reflux direction；
The kitchen sink is equipped with oscillator, cleaning solution bypass outlet and feed inlet, and can accommodate sieve pore external member；The feed inlet passes through
The two-way peristaltic pump of multiple tracks is connected with culture medium import, lye import, waste liquid outlet and sterilizing water inlet respectively.With prior art phase
Than the system and method for continuous production bacteria cellulose film provided by the invention continuously ferment in realization and produce bacteria cellulose
On the basis of film, production efficiency can be improved, and bacteria cellulose film easily extracts, is easy to wash after fermentation.
Meanwhile the system and method for continuous production bacteria cellulose film provided by the invention can be improved the utilization of fermentation liquid
Rate makes full use of the value of strain and fermentation liquid；And it can be by washing, neutralization and sterilization, cleaning process in the same apparatus
It completes, saves occupied space, manpower and the lye dosage of system, reduce environmental pollution, have both the feature of environmental protection and economy, be conducive to
It promotes and develops in this field.
In addition, by the mold of the good specific dimensions of fixed design in sieve pore external member, can also output predetermined size bacterium
Cellulose membrane has a extensive future.
In order to further illustrate the present invention, it is described in detail below by following embodiment.Following embodiment of the present invention
Culture medium used includes:
Solid slope culture medium: glucose 20g/L, yeast extract 5g/L, peptone 5g/L, Na2HPO48g/L, MgSO4
0.5g/L, agar 20g/L, 6.3,115 DEG C of sterilizing 20min of pH value are cooling stand-by；
Liquid seed culture medium: glucose 20g/L, yeast extract 5g/L, peptone 5g/L, Na2HPO48g/L, MgSO4
0.5g/L, ethyl alcohol 20g/L, adjusting pH value to 6.3 are stand-by after filtration sterilization；
Fermentation medium: carbon source be glucose 20g/L, nitrogen source be leaf of Moringa 5g/L and day open country peptone 5g/L, nitrogen source with
Carbon source mixes after cooling respectively at 115 DEG C of sterilizing 20min, adjusts pH value to 6.3, fermentation training is stored in after filtering and impurity removing degerming
It supports stand-by in base holding vessel.
Embodiment 1
The structural schematic diagram of the system for the continuous production bacteria cellulose film that embodiment 1 provides is shown in Figure 2, wherein 1
It is medium feed pipeline for fermentation vat, 2,3 be air intake conduit, and 4 be cleaning solution return line, and 5 be sieve pore external member, and 6 are
Sieve pore, 7 be windowing, and 8 be fortune device for hoisting, and 9 be kitchen sink, and 10 be peristaltic pump, and 11 be the first valve, and 12 be oscillator, and 13 be to wash
Liquid bypass outlet is washed, 14 be feed inlet, and 15 be the two-way peristaltic pump of multiple tracks, and 16 be culture medium import, and 17 be lye import, and 18 be useless
Liquid outlet, 19 be sterilizing water inlet, and 20 be the second valve, and 21 be circulating pump, and 22 be third valve, and 23 be circulation fluid return pipe
Road, 24 be the 4th valve, and 25 be the 5th valve.
The specification of fermentation vat (1) are as follows: 905mm × 605mm × 500mm, volume are about 0.274m3；Positive material is glass
Material, lap are 304 stainless steel materials, and surface stainless steel housing jacket heater is used for the maintenance of fermentation vat (1) temperature, temperature
Range is 20 DEG C~100 DEG C；Left side is attached to monitoring probe, and respectively temperature (temperature electrode), pH (pH electrode), dissolved oxygen amount is (molten
Oxygen electrode), residual sugar amount (residual sugar detecting electrode), OD value (OD value monitor electrode), part does not mark；Bottom is equipped with air intake duct
Road (3), air intake conduit (3) are equipped with the 5th valve (25) and connect with compressed air cylinder；Right upper portion is returned equipped with cleaning solution
Flow tube road (4), lower part are equipped with medium feed pipeline (2)；Marking on positive glass has, for observing bacterium
The growing state of cellulose membrane, and then determine the opportunity of harvest bacteria cellulose film.
The specification of sieve pore external member (5) are as follows: 900mm × 600mm × 250mm, using 304 stainless steel materials；Bottom surface is uniformly divided
Several diameters of cloth are the sieve pore of 10mm, culture medium (including the fermentation liquid and strain) filtering for adsorbing bacteria cellulose film
It returns in fermentation vat (1), also helps cleaning solution in subsequent laundering process and contacted with bacteria cellulose film；Side is close to bottom
Position is equipped with the windowing (7) of 600mm × 50mm, for taking out bacteria cellulose film when sieve pore external member (5) tilt.
The specification of kitchen sink (9) is 905mm × 605mm × 280mm, using 304 stainless steel materials；Left end is equipped with cleaning solution
Bypass outlet (13) is connected with cleaning solution return line (4), so that the cleaning solution in kitchen sink (9) be enable to return by cleaning solution
Flow tube road (4) is back to fermentation vat (1), and the cleaning solution return line (4) is successively arranged peristaltic pump along cleaning solution reflux direction
(10) and the first valve (11)；Right end is equipped with feed inlet (14), and the feed inlet (14) passes through the two-way peristaltic pump of multiple tracks (15) point
Not with culture medium import (16) (being pumped into culture medium), lye import (17) (being pumped into lye), waste liquid outlet (18) (pumping out waste liquid)
It is connected with sterilizing water inlet (19) (being pumped into aqua sterilisa)；Left bottom opens interface installation pH electrode, for detecting cleaning solution
PH value；Bottom is equipped with oscillator (12), specially cyclotron oscillation device, is able to drive kitchen sink (9) and does cyclotron oscillation, oscillatory process
In, cleaning solution washs bacteria cellulose film；The speed of oscillator (12) is 0~300rpm, oscillation amplitude Φ
26mm。
The continuous production of bacteria cellulose film is carried out using above system, specific work process is as follows:
1) before fermenting, the bacterial strain Komagataeibacter rhaeticus WM407-1 that will be deposited in glycerol tube
(CCTCC NO:M2016213) (bacterial strain discloses in the Chinese patent of Publication No. CN105802896A) strain is coated on
After activating 1 day on solid plate, picking is linked into seed culture medium, cultivates 2 days under the conditions of 150~200rpm, 30 DEG C, often
4h oscillation or stirring 5min, obtain seed culture fluid；
Then open the second valve (20) and third valve (22), start circulating pump (21), by 282L fermentation medium and
The above-mentioned seed culture fluid of 18L (by the inoculum concentration of volume fraction 6%) is pumped into fermentation vat (1), is closed circulating pump (21) and second
Valve (20), intermittent oscillation or stir culture 1 day at 30 DEG C, speed of agitator are 50~100rpm, and obtaining volume is 300L's
Fermentation liquid.
2) sieve pore external member (5) is placed in fermentation vat (1) by transporting device for hoisting (8), set the temperature of fermentation vat (1) as
30 DEG C, the 5th valve (25) and the 4th valve (24) are opened, starting circulating pump (21) mixes the fermentation liquid in fermentation vat (1)
It closes, makes dissolved oxygen amount 2mg/L, fermentation vat (1) is heated evenly, and maintains 30 DEG C of fermentation temperature；Be then shut off circulating pump (21) and
5th valve (25), ferments, and continues stationary culture, after culture 3 days, the thickness of obtained bacteria cellulose film reaches 3mm
When, sieve pore external member (5) is taken out from fermentation vat (1) by fortune device for hoisting (8), vacantly stands 10min, makes to be attached to bacterium fibre
The fermentation liquid and strain tieed up on plain film are returned in fermentation vat (1) as far as possible by the sieve pore of sieve pore external member (5) bottom surface；Then lead to
It crosses fortune device for hoisting (8) the sieve pore external member (5) with 1 bacteria cellulose film is transferred in kitchen sink (9).
3) start the two-way peristaltic pump of multiple tracks (15), 5L fermentation medium is pumped by culture medium import (16), starts oscillator
(12), 30min is vibrated under the hunting speed of 150rpm, is washed, make remaining fermentation liquid and bacterium in bacteria cellulose film
Kind is distributed in fermentation medium, obtains cleaning solution；Then it opens the first valve (11), starts peristaltic pump (10), by kitchen sink
(9) cleaning solution with original fermentation liquor and strain in all flows back into fermentation vat (1)；Restart the two-way peristaltic pump of multiple tracks
(15), the NaOH solution that 5L mass fraction is 1% is pumped by lye import (17), started oscillator (12), in the vibration of 150rpm
It swings and is vibrated under speed, neutralized and sterilized, under pH electrode detection when pH value no longer changes, start the two-way peristaltic pump of multiple tracks
(15), the waste liquid in kitchen sink (9) is all discharged by waste liquid outlet (18)；Restart the two-way peristaltic pump of multiple tracks (15), by going out
Bacterium water inlet (19) is pumped into 5L aqua sterilisa, starts oscillator (12), and 30min is vibrated under the hunting speed of 150rpm, carries out clear
It washes, then starts the two-way peristaltic pump of multiple tracks (15), the waste liquid in kitchen sink (9) is all discharged by waste liquid outlet (18)；Repeatedly
After cleaning, under pH electrode detection when pH stable is 7 or so, a bacteria cellulose film is obtained；By transporting device for hoisting (8)
Sieve pore external member (5) is taken out from kitchen sink (9), bacteria cellulose film skids off at the windowing (7) of sieve pore external member (5) after inclination
Into Storage Box, it is transferred out of aseptic area later.
Bacteria cellulose film, is laid in the top of lining cloth later, then covered again by first layer overlay lining cloth on the conveyor belt
One layer of lining cloth of lid, is then sent on film laminator and is squeezed, the most of moisture in bacteria cellulose film is squeezed out, Zhi Houxi
Fungin film is sent to the cutting of model and size needed for cutting process carries out together with lining cloth.
4) the initial water level that aqua sterilisa makes fermentation liquid be restored to step 1) is filled by circulating pump (21), another is sieved
Hole external member (5) is placed in fermentation vat (1), repeats step 2)~3), continuous production second opens film；11 times are so recycled to bacterium
The thickness of cellulose membrane increasess slowly (by the residual sugar amount in fermentation vat known to residual sugar detecting electrode, when residual sugar amount is lower than 0.5g/
L, fermentation liquid not enough produces next bacteria cellulose film in fermentation vat) stop production afterwards, amount to and obtains 12 bacteria celluloses
Film.
The dry weight of bacteria cellulose film is measured, the specific method is as follows: the washed process of bacteria cellulose film is washed to pH
Value is after 7, bacteria cellulose film is clear, colorless, to dry to constant weight in 80 DEG C of baking ovens, is weighed.
Measurement result ginseng is shown in Table 1.
The measurement result data of 1 Examples 1 to 3 of table and comparative example 1~2
As shown in Table 1, the system and method for the continuous production bacteria cellulose film that the embodiment of the present invention 1 provides, are done
The total weight of film is 4278.8g, total output 14.26g/L.
Embodiment 2
The system and method provided using embodiment 1, difference are: in step 3), start the two-way peristaltic pump of multiple tracks (15),
5L aqua sterilisa is pumped by sterilizing water inlet (19), starts oscillator (12), vibrates 30min under the hunting speed of 150rpm, into
Row washing, is distributed to remaining fermentation liquid and strain in bacteria cellulose film in aqua sterilisa, obtains cleaning solution；It is basic herein
On, in step 4), recycles 9 thickness to bacteria cellulose film and increases slowly (by fermentation vat known to residual sugar detecting electrode
Residual sugar amount, when residual sugar amount is lower than 0.5g/L, fermentation liquid not enough produces next bacteria cellulose film in fermentation vat) stop life afterwards
It produces, amounts to and obtain 10 bacteria cellulose films.
According to the measuring method in embodiment 1, the measurement result of table 1 is obtained.
As shown in Table 1, the system and method for the continuous production bacteria cellulose film that the embodiment of the present invention 2 provides, are done
The total weight of film is 3427.6g, total output 11.43g/L.
Comparative example 1
The system and method provided using embodiment 1, difference are: in step 2), disposably fermented, it is lasting to stand
Culture, the water level of 2 days detection fermentation liquids of every culture, and filling into aqua sterilisa by circulating pump (21) makes fermentation liquid be restored to step 1)
Initial water level；It, will by fortune device for hoisting (8) when the thickness of obtained bacteria cellulose film is not further added by after culture 16 days
Sieve pore external member (5) is taken out from fermentation vat (1)；Subsequent wash, neutralization and sterilization, cleaning process are same as Example 1；Obtain one
Open bacteria cellulose film.
According to the measuring method in embodiment 1, the measurement result of table 1 is obtained.
As shown in Table 1, it is 2318.8g, total output 7.73g/L that comparative example 1, which obtains the total weight of dry film,.
Also, it since the bacterium is aerobic bacteria, is in great demand when synthesizing bacteria cellulose film to oxygen, in fermentation process
Bacteria cellulose film is always formed in the gas-liquid interface of culture solution；With the extension of fermentation time, the increase of film thickness causes to seek
The transmitting for supporting substance and oxygen is affected, and the synthesis rate of bacteria cellulose film is decreased obviously.
Comparative example 2
The system and method provided using embodiment 1, difference are: in step 2), the thickness of obtained bacteria cellulose film
When degree reaches 3mm, sieve pore external member (5) are not taken out, but by way of sedimentation, the bacteria cellulose film is sunk into hair completely
Ferment culture medium (starting circulating pump, by the fermentation medium pump for bottom of pond portion of fermenting to the top of bacteria cellulose film), then by following
Ring pump (21) fills into the initial water level that aqua sterilisa makes fermentation liquid be restored to step 1), carries out the life of second bacteria cellulose film
It produces；After being repeated 6 times, the thickness of bacteria cellulose film stops production after increaseing slowly, and amounts to and obtains 7 bacteria cellulose films；So
Sieve pore external member (5) is taken out from fermentation vat (1) by fortune device for hoisting (8) afterwards；Subsequent wash, neutralization and sterilization, cleaning process
It is same as Example 1；Obtain 7 bacteria cellulose films.
According to the measuring method in embodiment 1, the measurement result of table 1 is obtained.
As shown in Table 1, it is 2433.2g, total output 8.11g/L that comparative example 2, which obtains the total weight of dry film,.
In addition, it is found that general inoculum concentration after being arranged by the production technology to bacteria cellulose film in the prior art
Minimum 5%, up to 10%；The time of bacteria cellulose film production is at least 5 days, and highest needs 8 days；Bacteria cellulose film
For single production, production capacity is about 0.857g/L.And the inoculum concentration of the embodiment of the present invention is 6%, is in lower inoculum concentration；Production
The time of one bacteria cellulose film is 2~3 days, and the time of relatively known bacteria cellulose film production time will be short；Bacterium
The production capacity of cellulose membrane can reach 12g/L；I.e. in relatively low inoculum concentration and can to obtain yield in the shorter time higher
Bacteria cellulose film.
The above description of the disclosed embodiments, enables those skilled in the art to implement or use the present invention.It is right
A variety of modifications of these embodiments will be readily apparent to those skilled in the art, and as defined herein one
As principle can realize in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention will
It will not be intended to be limited to the embodiments shown herein, and be to fit to consistent with the principles and novel features disclosed herein
Widest scope.
Claims (10)
1. a kind of system of continuous production bacteria cellulose film, comprising:
Fermentation vat；The fermentation vat is equipped with medium feed pipeline, air intake conduit and cleaning solution return line；
It can extend into the sieve pore external member inside fermentation vat；The sieve pore external member is the frame that bottom surface is evenly distributed with several sieve pores
Structure, side are equipped with windowing close to bottom position；
The fortune device for hoisting that the oscilaltion of sieve pore external member can be controlled and moved horizontally；
The kitchen sink being connected by cleaning solution return line with fermentation vat；The cleaning solution return line is along cleaning solution reflux direction
It is successively arranged peristaltic pump and the first valve；The kitchen sink is equipped with oscillator, cleaning solution bypass outlet and feed inlet, and can hold
Receive sieve pore external member；The feed inlet by the two-way peristaltic pump of multiple tracks respectively with culture medium import, lye import, waste liquid outlet and go out
Bacterium water inlet is connected.
2. system according to claim 1, which is characterized in that the medium feed pipeline is successively arranged along feedstock direction
Second valve, circulating pump and third valve；The circulating pump is additionally provided with the circulation fluid return line communicated with fermentation vat.
3. system according to claim 2, which is characterized in that the circulation fluid return line is equipped with the 4th valve.
4. system according to claim 1, which is characterized in that the air intake conduit is equipped with the 5th valve.
5. system according to claim 1, which is characterized in that the diameter of sieve pore is 5mm~15mm in the sieve pore external member,
The equal length of the side of the length and setting windowing of windowing, is highly 45mm~55mm.
6. a kind of method of continuous production bacteria cellulose film, which is characterized in that system described in claim 1 is used, including
Following steps:
A) fermentation liquid is mixed with air in the internal fermentation vat equipped with sieve pore external member, is fermented, in sieve pore external member
To the bacteria cellulose film with a thickness of 2mm~4mm, take out after sieve pore external member stands, be transferred in kitchen sink, using oscillator into
Row washing, the bacteria cellulose film after respectively obtaining cleaning solution and washing；Wherein, the cleaning solution is back in fermentation vat；
B) bacteria cellulose film after washing that step a) is obtained successively is neutralized and is sterilized, cleaned, obtain bacterial fibers
Plain film product；
Meanwhile the initial water level that aqua sterilisa or fermentation liquid make fermentation liquid be restored to step a) is filled into fermentation vat, then sending out
It is packed into sieve pore external member in ferment pond, repeats step a) and step b), continuous production bacteria cellulose film product.
7. according to the method described in claim 6, it is characterized in that, the temperature of fermentation described in step a) be 28 DEG C~32 DEG C,
Dissolved oxygen amount is 1mg/L~3mg/L.
8. according to the method described in claim 6, it is characterized in that, the process of washing described in step a) uses fermentation medium
Or aqua sterilisa；The hunting speed of the oscillator is 100rpm~200rpm, and duration of oscillation is 25min~35min.
9. according to the method described in claim 6, it is characterized in that, neutralization described in step b) and the process sterilized specifically:
Start the two-way peristaltic pump of multiple tracks, lye that 3L~10L mass fraction is 0.5%~1.5% is pumped into washing by lye import
Pond is washed, starts oscillator, is vibrated under the hunting speed of 100rpm~200rpm, neutralized and sterilized, in pH electrode detection
Under when pH value no longer changes, restart the two-way peristaltic pump of multiple tracks, the waste liquid in kitchen sink be all discharged by waste liquid outlet.
10. according to the method described in claim 6, it is characterized in that, the process of cleaning described in step b) specifically:
Start the two-way peristaltic pump of multiple tracks, 3L~10L aqua sterilisa is pumped into kitchen sink by aqua sterilisa import, starts oscillator,
25min~35min is vibrated under the hunting speed of 100rpm~200rpm, is cleaned, and the two-way peristaltic pump of multiple tracks is then started, it will
Waste liquid in kitchen sink is all discharged by waste liquid outlet；After being cleaned multiple times, when pH stable is 7 or so under pH electrode detection
When, obtain bacteria cellulose film product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111318049A (en) * | 2020-03-02 | 2020-06-23 | 黄介琪 | Fermented polysaccharide zymotic fluid extraction element |
| CN111593594A (en) * | 2020-05-28 | 2020-08-28 | 嘉兴学院 | Automatic production device for bacterial nano cellulose membrane |
| CN112063504A (en) * | 2020-09-25 | 2020-12-11 | 江西师范大学 | Curtain-hanging spray type bacterial cellulose gel culture device |
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| CN111318049A (en) * | 2020-03-02 | 2020-06-23 | 黄介琪 | Fermented polysaccharide zymotic fluid extraction element |
| CN111593594A (en) * | 2020-05-28 | 2020-08-28 | 嘉兴学院 | Automatic production device for bacterial nano cellulose membrane |
| CN112063504A (en) * | 2020-09-25 | 2020-12-11 | 江西师范大学 | Curtain-hanging spray type bacterial cellulose gel culture device |
| CN112063504B (en) * | 2020-09-25 | 2021-05-28 | 江西师范大学 | Curtain-hanging spray type bacterial cellulose gel culture device |
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