CN110201222B - Composite material for promoting bone to contain rubidium, titanium and tantalum and preparation method and application thereof - Google Patents
Composite material for promoting bone to contain rubidium, titanium and tantalum and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a rubidium-titanium-tantalum composite material for promoting bone formation, and a preparation method and application thereof. The material of the invention has good biocompatibility, no cytotoxicity, and good effect of inducing preosteoblast osteogenic differentiation, and can promote cell proliferation. The material can effectively solve the problems of poor compatibility of the bone repair material with a human body and the like in clinical application, and has wide application prospect in clinical bone repair.
Description
Technical Field
The invention belongs to the field of biomedical materials, and particularly relates to a rubidium-titanium-tantalum-containing composite material for promoting bone, and a preparation method and application thereof.
Background
Knee osteoarthritis, hip fracture and spine degenerative diseases are all orthopedic diseases with high incidence rate, and the incidence rate of the diseases is in the trend of rising and younger diseases along with the aggravation of aging of the population. The titanium-tantalum composite material is widely applied to the clinical orthopedics field due to good mechanical property, excellent biocompatibility and lower cost, and the titanium-tantalum composite material is used as a bone replacement material in common fracture patient treatment methods such as joint replacement, spine internal fixation and the like. However, since the titanium-tantalum composite material is a biological inert material, the titanium-tantalum composite material is mostly bonded mechanically with the bone of the human body after being implanted into the body, and cannot be bonded with the cells and tissues of the human body, the titanium-tantalum composite material has poor compressive and tensile strength and low bonding degree with the human body, so that the falling of the tissues and the implanted material is easy to occur, the operation fails, a patient needs to suffer great pain, and the medical cost is greatly increased. Therefore, the surface modification of the titanium-tantalum composite material to improve the biological activity is the key for improving the success rate of the operation.
With continuous exploration of rubidium ores in China and continuous progress of rubidium extraction technology, development and utilization of rubidium resources are continuously concerned by people. In our previous studies it was found that: the rubidium ions have a certain effect of promoting osteogenesis, can effectively induce the differentiation of preosteoblasts to osteoblasts, and improve the bioactivity of the titanium-tantalum composite material. In order to improve the functional effect of the bone repair material, rubidium salt can be added into the titanium-tantalum composite material, so that on one hand, the mechanical property of the titanium-tantalum composite material can be improved, and meanwhile, the titanium-tantalum composite material has good bioactivity, and the bone repair material with clinical application value is formed.
However, for the purpose of the present time, no report on rubidium doping of titanium-tantalum composite materials is found.
Disclosure of Invention
Aiming at the problems that the medical titanium-tantalum composite material in the prior art has poor bioactivity and is easy to cause operation failure in practical clinical application, the invention aims to provide a rubidium-containing titanium-tantalum composite material for promoting bones with excellent mechanical property and bioactivity, and a preparation method and application thereof.
In order to solve the technical problems, the invention adopts the following technical scheme:
the rubidium-titanium-tantalum-containing composite material for promoting bone formation is obtained by doping rubidium into a titanium-tantalum composite material on the surface, and the titanium-tantalum composite material is formed by sintering discharge plasma.
The inventor surprisingly finds that the titanium tantalum composite material sintered by SPS has the optimal bone-promoting capacity after being doped with rubidium, wherein the doping amount of the titanium tantalum composite material prepared by SPS sintering is low compared with that of vacuum sintering, but the doping amount is most suitable for promoting bone formation, so that the optimal bone-promoting performance can be obtained.
The preferable scheme is as follows: in the titanium-tantalum composite material, the ratio of titanium to tantalum is 1:10-30 by atomic ratio.
More preferably, in the titanium-tantalum composite material, titanium and tantalum are 1:20-30 in terms of atomic ratio.
In the scheme of the invention, the atomic ratio of the titanium-tantalum composite material is within the range of the invention, and the titanium-tantalum composite material has the optimal mechanical property and friction and abrasion resistance which are matched with the mechanical property of human bones.
The invention relates to a preparation method of a rubidium-titanium-tantalum-containing composite material for promoting bone, which comprises the following steps: performing discharge plasma sintering on titanium-tantalum alloy powder to obtain a titanium-tantalum composite material, sequentially performing sand blasting treatment, acid treatment and alkaline heat treatment on the titanium-tantalum composite material to obtain a titanium-tantalum composite material with a surface treated, then soaking the titanium-tantalum composite material with the surface treated in a rubidium salt solution for reaction to obtain a rubidium salt treated titanium-tantalum composite material, and calcining the rubidium salt treated titanium-tantalum composite material to obtain the rubidium-containing titanium-tantalum composite material.
In the technical scheme of the invention, in order to smoothly dope rubidium and obtain better bone performance, multiple pretreatments of sand blasting, acid treatment and alkali heat treatment are carried out before rubidium doping, wherein the sand blasting can form a micron-sized rough surface on the surface of the titanium-tantalum composite material. The acid treatment can form micro-nano-scale holes on the surface of the titanium-tantalum composite material, can effectively enlarge the contact area of cells and the titanium-tantalum composite material, is beneficial to the adhesion of the cells on the surface of an implant, simultaneously enhances the bonding strength of a soft tissue interface, can effectively improve the biological performance of the titanium-tantalum composite material, and enlarges the effective reaction area of the subsequent alkali heat treatment and ion exchange. The titanium-tantalum composite material surface after the alkali heat treatment can further generate micron-nano composite holes similar to bone structures, is a good surface modification method, can prepare a bioactive coating with high compatibility and high adhesion with bones, and cannot be realized by other surface modification methods. The titanate layer with the optimal thickness can be obtained by using the alkali heat treatment on the titanium-tantalum composite material, the titanium-tantalum composite material with the gradient change of mechanical and corrosion resistance can be obtained, and the bioactivity of the titanium-tantalum composite material can be optimized. According to the invention, by combining sand blasting, acid treatment and alkali heat treatment with ion exchange and high-temperature calcination, rubidium can be effectively doped into the surface of the titanium-tantalum composite material, so that the bone formation promoting titanium-tantalum composite material with clinical application value is obtained.
In the invention, three pretreatment modes are mutually cooperated, the rings are buckled, for example, if sand blasting is not carried out, acid treatment (acid etching) is carried out, alkali treatment and rubidium doping treatment are carried out, the surface can not form the surface appearance which is most beneficial to osteoblast attachment and can effectively induce the differentiation of the preosteoblasts to the osteoblasts, and the titanium-tantalum surface which is subjected to the alkali treatment and the rubidium doping treatment after the sand blasting and the acid etching is of a micro-nano composite hole structure and is beneficial to inducing the differentiation of the preosteoblasts to the osteoblasts. The sand blasting is a physical means, micron-sized holes can be endowed to the titanium-tantalum composite material, the acid etching is a chemical means, and a nano-scale structure is formed on the micron-sized surface after the sand blasting, so that osteogenic differentiation is effectively regulated and controlled.
In the preferable scheme, the temperature of the spark plasma sintering is 950-.
More preferably, the temperature of the spark plasma sintering is 1000-1300 ℃, the sintering time is 4-6min, the sintering pressure is 30-60MPa, and the temperature rise speed is 50-80 ℃/min.
For the sand blasting process, the conventional prior art process is adopted, and the sand blasting raw material is preferably quartz sand.
Preferably, the acid treatment process is to soak the titanium-tantalum composite material subjected to sand blasting treatment in an acid solution and treat the titanium-tantalum composite material at 60-85 ℃ for 20-30 h.
Preferably, the acid solution is a mixed solution obtained by mixing a hydrochloric acid solution, a sulfuric acid solution and water according to a volume ratio of 7:7: 7-8; the mass fraction of dissolved HCl in the hydrochloric acid solution is 37-39%, and the mass fraction of sulfuric acid in the sulfuric acid solution is 95-98%.
The inventor finds that the acid etching effect is best when the hydrochloric acid solution, the sulfuric acid solution and the water are mixed according to the volume ratio of the invention.
Preferably, the alkali heat treatment process is to soak the titanium-tantalum composite material subjected to acid treatment in an alkali solution and treat the titanium-tantalum composite material at 60-85 ℃ for 20-30 h.
In a preferred embodiment, the alkali solution isOH-The concentration of (b) is 2-5 mol/L.
As a further preference, OH in the alkali solution-The concentration of (b) is 4-5 mol/L.
Preferably, the alkali solution is at least one selected from a sodium hydroxide solution, a potassium hydroxide solution or a strontium hydroxide solution.
Cleaning and drying the titanium-tantalum composite material subjected to alkali heat treatment by using deionized water; and obtaining the titanium-tantalum composite material with the treated surface.
In a preferred embodiment, the rubidium salt is at least one selected from the group consisting of rubidium chloride, rubidium nitrate and rubidium sulfate.
Preferably, the rubidium salt solution has a rubidium ion concentration of 1 to 5 mol/L.
More preferably, the rubidium salt solution has a rubidium ion concentration of 2.5 to 5 mol/L.
Preferably, the titanium-tantalum composite material subjected to surface treatment is soaked in rubidium salt solution for reaction at the temperature of 60-85 ℃ for 20-30 hours.
Preferably, the calcining temperature is 550-700 ℃, and the time is 1-4 h.
The invention relates to an application of a rubidium-titanium-tantalum-containing composite material for promoting bones, which is used as a bone replacement material.
The invention has the beneficial effects that:
the invention provides a rubidium-titanium-tantalum-containing composite material for promoting bone formation, and a preparation method and application thereof for the first time.
In the invention, in order to obtain better bone-promoting performance and optimal rubidium-doping amount, in the preparation process of the rubidium-containing titanium-tantalum composite material for promoting bone, triple pretreatment of sand blasting, acid etching and alkali heat treatment is firstly carried out on the titanium-tantalum composite material, wherein the sand blasting endows micron-sized holes on the titanium-tantalum composite material, the acid etching forms a nano-scale structure on the micron-sized surface after the sand blasting, osteogenic differentiation is effectively regulated and controlled, the alkali heat treatment enables the surface of the titanium-tantalum composite material to further generate micron-nano composite holes similar to the bone structure, and a titanate layer with optimal thickness is formed for ion exchange and rubidium doping. Through the mutual cooperation of the three pretreatments, the rubidium-titanium-tantalum-containing composite material with the optimal appearance and the optimal rubidium doping amount is finally obtained, so that the excellent bone performance promotion performance is obtained.
The rubidium-titanium-tantalum-containing composite material for promoting bone prepared by the method has good biocompatibility, can effectively solve the problems of poor biocompatibility and the like of the titanium-tantalum composite material in clinical application, and has wide application prospect in clinical bone repair.
The technical solution of the present invention is further specifically described below with reference to the accompanying drawings and specific embodiments.
Drawings
Fig. 1 is a graph showing XPS detection results of rubidium elements of samples of examples.
FIG. 2 is a graph showing the results of the day two and day four CCK-8 tests with MC3T3 cells plated on samples from different examples; wherein A is the sample obtained in comparative example 1, B is the sample obtained in example 1, C is the sample obtained in example 2, D: the sample obtained in comparative example 2, E: the sample obtained in comparative example 3, and F: the sample obtained in comparative example 4.
FIG. 3 is a graph of results of sirius red staining 10 days after plating MC3T3 cells on samples from different examples; wherein A is the sample obtained in comparative example 1, B is the sample obtained in example 1, and C is the sample obtained in example 2.
Detailed Description
Example 1
Preparing titanium-tantalum alloy powder, wherein in the titanium-tantalum alloy powder, the ratio of titanium: tantalum is 1: 30; then placing the titanium-tantalum alloy powder in a discharge plasma sintering furnace for sintering, and obtaining the titanium-tantalum composite material after sintering, wherein the sintering procedure is as follows: the heating speed is 70 ℃/min, the sintering temperature is 1000 ℃, and the sintering time is 5 min; the sintering pressure is 60 MPa.
The titanium-tantalum composite material is subjected to sand blasting treatment and then placed in an acid solution to be subjected to acid treatment at 70 ℃ for 28h, wherein the acid solution is obtained by mixing hydrochloric acid (mass fraction: 38%) and sulfuric acid (mass fraction: 96%) in a volume ratio of 7:7: 8. Placing the titanium-tantalum composite material subjected to acid treatment in a 4M potassium hydroxide solution, and carrying out alkali heat treatment at 70 ℃ for 28 h; and then washing the titanium-tantalum composite material subjected to alkaline heat treatment by using deionized water to remove a solution on the surface of the titanium-tantalum composite material, drying and blow-drying to obtain a surface-treated titanium-tantalum composite material, soaking the surface-treated titanium-tantalum composite material in a 5M rubidium salt solution for ion exchange treatment at the treatment temperature of 70 ℃ for 28 hours, and finally calcining the rubidium salt-treated titanium-tantalum composite material at high temperature at the calcination temperature of 600 ℃ for 4 hours in furnace air cooling to obtain the rubidium-containing titanium-tantalum composite material.
To evaluate the composition of the rubidium titanium tantalum composite material for promoting bone formation, XPS was used to perform elemental analysis on the rubidium titanium tantalum composite material for promoting bone formation, and fig. 1 shows the XPS of the rubidium titanium tantalum composite material obtained in example 1, which shows that rubidium element has been successfully incorporated.
In order to evaluate the osteogenesis condition of the cells, the proliferation condition of the cells is detected (cck8 reagent and characterization), and the collagen secretion condition of the preosteoblasts (sirius red staining) is carried out respectively, the collagen secretion is an important mark for differentiation from early to middle stages to the osteoblasts, and the collagen secretion condition and the osteoblasts jointly illustrate the osteogenesis condition of the cells from different angles.
CCK-8 measures proliferation of cells
In order to evaluate the toxicity of the rubidium-titanium-tantalum-containing bone promoting composite material on cells and the proliferation capacity of the cells, the obtained rubidium-titanium-tantalum-containing bone promoting composite material is subjected to cell proliferation and toxicity (CCK-8) evaluation, and the proliferation condition of the cells is represented by a CCK-8 kit. The experimental process is as follows:
(1) co-culturing the bone promoting rubidium-titanium-tantalum composite material and mouse preosteoblasts MC3T3 for three days, and then absorbing and removing the complete culture medium;
(2) adding 10% CCK-8 solution into each well to interact with cells, and incubating for 1h at 37 ℃;
(3) after incubation, transfer 100 μ L of solution from each well to a 96-well plate;
(4) and detecting the absorbance of the solution by a microplate reader, wherein the detection wavelength is 450 nm.
The result is shown as B in FIG. 2. The figure shows that the cell activity of the mouse preosteoblasts MC3T3 inoculated in the bone-promoting rubidium-titanium-tantalum-containing composite material is stronger. The results show that the composite material for promoting the bone to contain rubidium, titanium and tantalum has good biocompatibility, no cytotoxicity and excellent cell proliferation capacity.
Detection of preosteoblastic collagen secretion by sirius red staining
In order to evaluate the capacity of the rubidium-titanium-tantalum-containing composite material for promoting bone to induce osteogenesis differentiation on preosteoblasts, the obtained rubidium-titanium-tantalum-containing composite material for promoting bone is subjected to collagen secretion detection, sirius red staining is adopted for evaluation, the increase of collagen secretion is an important mark for the early differentiation of preosteoblasts to osteoblasts, and the test process is as follows:
MC3T3-E1 cells were seeded onto the surface of the material and after 10 days of culture in complete medium, collagen synthesized inside the cells was analyzed using a sirius red staining kit. The method comprises the following specific steps:
(1) the medium was discarded and the cells were washed 2 times with PBS for 5min each.
(2) Add 4% paraformaldehyde to each well and fix at room temperature for 15 min.
(3) The fixative was discarded and the cells were washed 3 times with PBS for 5min each time.
(4) PBS was discarded, 200. mu.L of sirius red staining solution was added to each well, and staining was performed at room temperature for 18 h.
(5) The sirius red stain is discarded, and the cells are repeatedly washed by deionized water until the washing solution does not turn red.
(6) Observed using an inverted microscope (DFC420C, lycra, germany) and photographed.
(7) Add 200 μ L0.2M NaOH per well: methanol 1:1 and the absorbance at 520nm was measured.
The result is shown as B in fig. 3. The figure shows that the collagen content secreted by the preosteoblasts of mice inoculated with the matrix type promoting the bone to contain the rubidium, titanium and tantalum composite material is higher than that of a control group.
The results show that the rubidium, titanium and tantalum-containing composite material for promoting bone has good biocompatibility, no cytotoxicity, excellent cell proliferation and good effect of inducing preosteoblast osteogenic differentiation, which indicates that the capacity of promoting bone of the embodiment 1 is excellent!
Example 2
Preparing titanium-tantalum alloy powder, wherein in the titanium-tantalum alloy powder, the ratio of titanium: tantalum is 1: 20; then placing the titanium-tantalum alloy powder in a discharge plasma sintering furnace for sintering, and obtaining the titanium-tantalum composite material after sintering, wherein the sintering procedure is as follows: the heating rate is 50 ℃/min, the sintering temperature is 1300 ℃, and the sintering time is 5 min; the sintering pressure is 30 MPa.
The titanium-tantalum composite material is subjected to sand blasting treatment and then placed in an acid solution to be subjected to acid treatment for 24 hours at 65 ℃, wherein the acid solution is obtained by mixing hydrochloric acid (mass fraction: 37% written specific value) and sulfuric acid (mass fraction: 95% written specific value) with water according to the volume ratio of 7:7: 8. Placing the titanium-tantalum composite material subjected to acid treatment in a 5M sodium hydroxide solution, and carrying out alkali heat treatment for 24 hours at 65 ℃; and then washing the titanium-tantalum composite material subjected to alkaline heat treatment by using deionized water to remove a solution on the surface of the titanium-tantalum composite material, drying and blow-drying to obtain a surface-treated titanium-tantalum composite material, soaking the surface-treated titanium-tantalum composite material in a 2.5M rubidium salt solution for ion exchange treatment at 65 ℃ for 24 hours, and finally performing high-temperature calcination on the rubidium salt-treated titanium-tantalum composite material at 650 ℃ for 2 hours, and performing furnace air cooling to obtain the rubidium-containing titanium-tantalum composite material.
In order to evaluate the toxicity of the rubidium-titanium-tantalum-containing composite material for promoting bone on cells, the obtained rubidium-titanium-tantalum-containing composite material for promoting bone is subjected to cell proliferation and toxicity (CCK-8) evaluation, and the proliferation condition of the cells is represented by a CCK-8 kit. The experimental process is as follows:
(1) co-culturing the bone promoting rubidium-titanium-tantalum composite material and mouse preosteoblasts MC3T3 for three days, and then absorbing and removing the complete culture medium;
(2) adding 10% CCK-8 solution into each well to interact with cells, and incubating for 1h at 37 ℃;
(3) after incubation, transfer 100 μ L of solution from each well to a 96-well plate;
(4) and detecting the absorbance of the solution by a microplate reader, wherein the detection wavelength is 450 nm.
The results are shown in fig. 2C. The figure shows that the cell activity of the mouse preosteoblasts MC3T3 inoculated in the bone-promoting rubidium-titanium-tantalum-containing composite material is stronger. The results show that the composite material for promoting the bone to contain rubidium, titanium and tantalum has good biocompatibility, no cytotoxicity and excellent cell proliferation capacity.
In order to evaluate the capacity of the rubidium-titanium-tantalum-containing composite material for promoting bone to induce osteogenesis differentiation on preosteoblasts, the obtained rubidium-titanium-tantalum-containing composite material for promoting bone is subjected to collagen secretion detection, sirius red staining is adopted for evaluation, the increase of collagen secretion is an important mark for the early differentiation of preosteoblasts to osteoblasts, and the test process is as follows:
MC3T3-E1 cells were seeded onto the surface of the material and after 10 days of culture in complete medium, collagen synthesized inside the cells was analyzed using a sirius red staining kit. The method comprises the following specific steps:
(1) the medium was discarded and the cells were washed 2 times with PBS for 5min each.
(2) Add 4% paraformaldehyde to each well and fix at room temperature for 15 min.
(3) The fixative was discarded and the cells were washed 3 times with PBS for 5min each time.
(4) PBS was discarded, 200. mu.L of sirius red staining solution was added to each well, and staining was performed at room temperature for 18 h.
(5) The sirius red stain is discarded, and the cells are repeatedly washed by deionized water until the washing solution does not turn red.
(6) Observed using an inverted microscope (DFC420C, lycra, germany) and photographed.
(7) Add 200 μ L0.2M NaOH per well: methanol 1:1 and the absorbance at 520nm was measured.
The results are shown in fig. 3C. It is shown that the amount of collagen secreted by the preosteoblasts inoculated into mice having bone comprising rubidium, titanium and tantalum composite material is higher than that of the control group.
The results show that the rubidium, titanium and tantalum-containing composite material for promoting bone has good biocompatibility, no cytotoxicity, excellent cell proliferation and good effect of inducing preosteoblast osteogenic differentiation, which indicates that the capacity of promoting bone of the embodiment 2 is excellent!
Comparative example 1
Other conditions of this comparative example were the same as those of example 2 except that the acid solution used in the acid treatment process was obtained by mixing hydrochloric acid (mass fraction: 37% written specific value) and sulfuric acid (mass fraction: 95% written specific value) in water at a volume ratio of 1:1: 8.
In order to evaluate the osteogenesis condition of the cells, the sample of comparative example 1 was subjected to the proliferation condition test of the cells (cck8 reagent characterization), and the collagen secretion condition of the pre-osteoblasts (sirius red staining), respectively, and the test methods were consistent with the examples, and as a result, the proliferation condition of the cells was shown as a in fig. 2, the collagen secretion ability of the osteoblasts was shown as a in fig. 3, and the results showed that both were weaker than those of examples 1-2, and it could be confirmed that the titanium-tantalum composite material in the examples had a better bone-promoting ability.
Comparative example 2
The other conditions are the same as those of the example 2, except that ion exchange is not carried out, and rubidium is not doped, so that the titanium-tantalum composite material subjected to surface treatment is obtained.
When the sample obtained in comparative example 2 is subjected to cell proliferation assay (cck8 reagent and characterization), the test method is consistent with the example, and the result is shown in fig. 2D, which shows that the proliferation capacity of the mouse preosteoblasts inoculated in the titanium-tantalum composite material in comparative example 2 is obviously lower than that of the titanium-tantalum composite material in examples 1-2, and the titanium-tantalum composite material in the examples has better bone-promoting capacity.
Comparative example 3
Other conditions were the same as those in example 2, and only the sand blast treatment for acid treatment was not performed, but the surface treatment of the resulting titanium tantalum composite material was directly subjected to the alkali heat treatment.
The sample obtained in comparative example 3 was subjected to cell proliferation assay (cck8 reagent and characterization), the assay method of which was in accordance with the examples,
the results are shown in FIG. 2E. It is shown that the proliferation capacity of the mouse preosteoblasts inoculated in the rubidium-titanium-tantalum-containing composite material in the comparative example 3 is obviously lower than that of the mice in the examples 1-2, and the titanium-tantalum composite material in the examples can prove to have better bone-promoting capacity.
Comparative example 4
The other conditions are consistent with those of the example 2, and only the preparation method of the titanium-tantalum composite material is vacuum sintering, and the vacuum sintering comprises the following specific steps:
preparing titanium-tantalum alloy powder, wherein in the titanium-tantalum alloy powder, the ratio of titanium: tantalum is 1: 20; then placing titanium-tantalum alloy powder into a cold isostatic press for pressing and forming, wherein the pressing pressure is 200MPa, the pressing time is 4min, then placing the pressed and formed green body into vacuum sintering for sintering, and controlling the vacuum degree to be 10-4Pa, the temperature of vacuum sintering is 1200 ℃, the sintering time is 2h, and the temperature rise speed is 45 ℃/min.
The sample obtained in comparative example 4 was subjected to cell proliferation assay (cck8 reagent and characterization), the assay method of which was in accordance with the examples,
the results are shown in FIG. 2 as F. It is shown that the proliferation capacity of the mouse preosteoblasts inoculated in the rubidium-titanium-tantalum-containing composite material in the comparative example 4 is obviously lower than that of the mice in the examples 1-2, and the titanium-tantalum composite material in the examples can prove to have better bone-promoting capacity.
Claims (4)
1. A preparation method of a rubidium-titanium-tantalum composite material for promoting bone to contain rubidium, titanium and tantalum is characterized by comprising the following steps: performing discharge plasma sintering on titanium-tantalum alloy powder to obtain a titanium-tantalum composite material, wherein in the titanium-tantalum composite material, titanium is in a ratio of 1:10-30 in terms of atomic ratio, performing sand blasting treatment, acid treatment and alkaline heat treatment on the titanium-tantalum composite material in sequence to obtain a titanium-tantalum composite material with a surface treated, then soaking the titanium-tantalum composite material with the surface treated in a rubidium salt solution for reaction to obtain a rubidium salt treated titanium-tantalum composite material, and calcining the rubidium salt treated titanium-tantalum composite material to obtain the rubidium-containing titanium-tantalum composite material;
the alkali heat treatment process comprises the steps of soaking the titanium-tantalum composite material subjected to acid treatment in an alkali solution, and treating for 20-30h at the temperature of 60-85 ℃, wherein OH in the alkali solution-In a concentration of2-5mol/L, wherein the alkali solution is at least one of sodium hydroxide, potassium hydroxide or strontium hydroxide solution;
the temperature of the discharge plasma sintering is 950-;
the acid treatment process comprises the steps of soaking the titanium-tantalum composite material subjected to sand blasting treatment in an acid solution, and treating at 60-85 ℃ for 20-30 h;
the calcining temperature is 550-700 ℃, and the time is 1-4 h.
2. The method as claimed in claim 1, wherein the acid solution is a mixed solution obtained by mixing hydrochloric acid solution, sulfuric acid solution and water at a volume ratio of 7:7: 7-8; the mass fraction of dissolved HCl in the hydrochloric acid solution is 37-39%, and the mass fraction of sulfuric acid in the sulfuric acid solution is 95-98%.
3. The method of claim 1, wherein said rubidium salt is selected from at least one of rubidium chloride, rubidium nitrate, and rubidium sulfate, and a rubidium ion concentration in said rubidium salt solution is from 1 to 5 mol/L.
4. Use of a rubidium titanium tantalum composite material for promoting bone formation, prepared by the preparation method according to any one of claims 1-3, characterized in that: the bone promoting rubidium-titanium-tantalum-containing composite material is applied as a bone replacement material.
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