CN110200274A - Utilize the method and application of polyphyll red rose slag preparation environmental protection ferment - Google Patents
Utilize the method and application of polyphyll red rose slag preparation environmental protection ferment Download PDFInfo
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- CN110200274A CN110200274A CN201910528319.4A CN201910528319A CN110200274A CN 110200274 A CN110200274 A CN 110200274A CN 201910528319 A CN201910528319 A CN 201910528319A CN 110200274 A CN110200274 A CN 110200274A
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
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- 239000003344 environmental pollutant Substances 0.000 claims abstract description 6
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- 150000002989 phenols Chemical class 0.000 description 5
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- 238000001514 detection method Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
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- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
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- 244000178870 Lavandula angustifolia Species 0.000 description 2
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- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
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- 244000037364 Cinnamomum aromaticum Species 0.000 description 1
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- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
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- 244000199885 Lactobacillus bulgaricus Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- 241000205407 Polygonum Species 0.000 description 1
- 244000046146 Pueraria lobata Species 0.000 description 1
- 235000010575 Pueraria lobata Nutrition 0.000 description 1
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- 241001165494 Rhodiola Species 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 240000006066 Rosa rugosa Species 0.000 description 1
- 235000000659 Rosa rugosa Nutrition 0.000 description 1
- 241000698291 Rugosa Species 0.000 description 1
- 240000005319 Sedum acre Species 0.000 description 1
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- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
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- 238000002835 absorbance Methods 0.000 description 1
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- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940106691 bisphenol a Drugs 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
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- 235000012907 honey Nutrition 0.000 description 1
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- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- BZDIAFGKSAYYFC-UHFFFAOYSA-N manganese;hydrate Chemical compound O.[Mn] BZDIAFGKSAYYFC-UHFFFAOYSA-N 0.000 description 1
- 235000013575 mashed potatoes Nutrition 0.000 description 1
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- 229910052757 nitrogen Inorganic materials 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
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- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
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- 238000010563 solid-state fermentation Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0059—Catechol oxidase (1.10.3.1), i.e. tyrosinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0061—Laccase (1.10.3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
- C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
- C12Y110/03001—Catechol oxidase (1.10.3.1), i.e. tyrosinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
- C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
- C12Y110/03002—Laccase (1.10.3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
- C12Y111/01—Peroxidases (1.11.1)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
Abstract
A kind of method and application using polyphyll red rose slag preparation environmental protection ferment, it is characterized in that it is made of following steps: adjustment, the environmental protection ferment fermentation, the preparation of environmental protection ferment of polyphyll red rose slag water content.Preparing degradation of phenol pollutant environmental protection ferment using polyphyll red rose slag may be implemented its higher value application, this is for the rose slag resource utilization important in inhibiting in rose process.
Description
Technical field
The present invention relates to a kind of preparation method of environmental protection ferment ferment more particularly to a kind of utilization polyphyll red rose slag systems
The method and application of standby environmental protection ferment.
Background technique
Rose (Rosa rugosa) it is under the jurisdiction of rosaceae (Rosaceae) Rosa, there is long cultivation to go through in China
History, principal item are " polyphyll rose " and " bitter water rose ", and domestic widely distributed, mainly there are Shandong Pingyin, north in major production areas
The ground such as capital Miaofeng Shan Mountain, Gansu misery, the Shangshui in Henan and Yanling, hotan and Sichuan Meishan.Wherein Shandong Pingyin is China
Famous " township of rose ", the rose plant gross area are more than 60,000 mu, and Zhan Quanguo cultivated area is more than 30%, produce fresh rose flower 1 per year
More than ten thousand tons.Rose slag is the residual fraction extracted after essential oil, rich in a variety of function such as protein, amino acid, dietary fiber, polyphenol
Energy property and nutritional ingredient, suitable for a variety of downstream product of exploitation.
CN201410652136.0 proposes a kind of method for producing laccase enzyme solution and powdery laccase based on Chinese medicine dreg,
Fermentation substrate used is the arbitrary proportion mixing dregs of a decoction of polygonum cuspidate, the rheum officinale dregs of a decoction or the two.It ferments mainly using in the dregs of a decoction
Carbon source and nitrogen source etc., the effect for generating the phenols especially phenol contaminant degradation enzymes such as laccase is induced there is no phenols.
CN201410058416.9 discloses one kind using Chinese medicine as the thick cap bore bacterium preparing laccase by solid-state fermentation of matrix bristle and fermentation residue
The method of comprehensive utilization, with Chinese medicine (predominantly one of Radix Glycyrrhizae, pueraria lobata, Radix Astragali, hawthorn, cassia seed and Chinese ephedra) for solid-state base
Matter, the main microelement substitution exogenous copper ions induction using in Chinese medicine produce laccase.Its described microelement simultaneously undeclared is
Which kind of substance, therefore have no way of judging that it induces principle and effect.It is main that CN201410486163.5, which discloses one kind with lychee exocarp,
The environmental protection ferment production method of raw material, with lychee exocarp, mangosteen skin, lemon peel, longan, durian core, jackfruit core, Grape Skin, spinach
Trailing plants skin and milk are raw material, and yeast fermentation is added；After adding water to mix distillation rose, aloe, safflower, root of kirilow rhodiola, lavender
Distillate is collected, and the mashing of remaining mixture plus yeast are fermented；Lactic acid is added after two kinds of fermentation liquids are mixed with distillate
Bacterium obtains environmental protection ferment after fermenting again.Its complex manufacturing process, it is at high cost；And with saccharomycete and lactobacillus-fermented.Its process master
If product is mainly alcohol using the glucide in a variety of pericarps using fermentation；It is rose used, aloe, safflower, red
The raw materials such as red-spotted stonecrop, lavender are mainly used for distillation and obtain the distillate of direction smell for adjusting the smell of ferment.Utilize this
Method environmental protection ferment obtained is being not for degrading phenol especially phenol pollutant.CN201811006916.2 is public
The production method for opening a kind of pure-blood ferment rose enzyme.This application belongs to the edible ferment field of preparation, and method is with rose
For primary raw material, white granulated sugar and water is added to mix thoroughly, segmentation inoculation patented strain lactobacillus plantarum and saccharomyces cerevisiae, in closed container
Fermentation, the rose enzyme stoste of acquisition.Lactobacillus plantarum is accessed since white granulated sugar is the carbon source easily utilized, and in fermentation process
And saccharomyces cerevisiae, main purpose are to utilize the larger (weight ratio of rose raw material and white granulated sugar, pure water of white granulated sugar additive amount
Example is 1-2: 1-2: 6-8, and when raw material is rose dried flower, the weight ratio of rose raw material and white granulated sugar, pure water is 0.1-
0.2: 1-2: 7.8-8.9) it will lead to production alcohol and lactic acid production be big, other microorganisms is inhibited to grow, it can not be using in rose
Phenolic substances be substrate generate phenols degradation enzyme system.Chinese invention patent application CN201610759933.8 provides a kind of rose
Rare ferment and preparation method thereof, the raw material according to parts by weight include: 10 parts of water, 3 parts of roseleaf, sugar 1 part prepare it is closed
Ferment 3 months acquisition ferment.Chinese invention patent application CN201610279203.8 provides a kind of producer of rose enzyme
Method belongs to food, field of health care products.This method comprises the following steps: 1) picking, the early morning before sunrise picks roseleaf；
2) it sorts, picks out complete roseleaf from the roseleaf that step 1 picking obtains；3) it ferments, by the rose of step 2
Valve is mixed with white granulated sugar, pure water according to the weight ratio of 1:1-2:5-8, addition honey, Pancreatinum and Flos Rosae Rugosas pollen conduct
Auxiliary material is uniformly mixed, and is placed in sealing container, is saved 120-180 days under 18-25 degrees Celsius, shading environment after sealing,
Midway is opened container 1-3 times, opens container every time and all the mixture in container is sufficiently stirred；4) it is separated by solid-liquid separation, obtains
Rose enzyme stoste；5) it sterilizes canned, obtains rose enzyme canned products.The patent application is used with CN201610759933.8
To a large amount of white granulated sugar (more than 10%, it might even be possible to more than 30% sugar content), under this high concentration sugar content, mainly ferment
Female bacterium is using sugared producing and ethanol, so that other microorganisms be inhibited to grow, the purpose is to produce the preferable food-grade ferment drink of taste
Expect and nonproductive environmental protection ferment.
Summary of the invention
Limitation and deficiency for the prior art, the object of the present invention is to provide prepare ring using polyphyll red rose slag
Protect the method and application of ferment.This method utilize using polyphyll rose slag as unique raw material, using indigenous microorganism therein into
Row fermentation prepares the environmental protection ferment of degrading phenol pollutant, have it is at low cost, rose gives off a strong fragrance, suitable for the excellent of large-scale production
Point.
The present invention provides a kind of methods and application using polyphyll red rose slag preparation environmental protection ferment, it is characterized in that it
Using polyphyll red rose slag be raw material, with polyphenol substance therein induction polyphenol degradation enzyme (such as laccase, peroxidase and
Tyrosinase etc.) it generates.Specifically it is made of following steps:
(1) allotment of environmental protection ferment fermentation substrate: being adjusted to water content 60-90wt% for polyphyll red rose slag, obtains environmentally friendly ferment
Plain fermentation substrate.
(2) it ferments: environmental protection ferment fermentation substrate being fitted into closed container, is fermented under the conditions of 20-40 DEG C, during which
Deflation 2-3 times.Fermentation time 15-45 days, obtain environmental protection ferment crude product.
(3) it filters: being filtered environmental protection ferment crude product using 100 mesh filter clothes, obtain environmental protection ferment product after standing clarification.
The polyphyll red rose slag refers to using polyphyll red rose using obtained after cooking process extraction essential oil
Flower slag refers in particular to extract after essential oil protein content 7-15%, Determination of Polyphenols 7-9%, the weight of water content 40-95% in dry matter
Valve red rose slag.
A kind of application using polyphyll red rose slag preparation environmental protection ferment, it is for producing degradation of phenol pollutant ring
Protect ferment.
The beneficial effects of the present invention are: being there is no at present with polyphyll red rose slag is raw material production degradation of phenol pollutant
The report of environmental protection ferment.The present invention creatively utilizes protein, sugar, cellulose, lignin and polyphenol object in rose slag
Matter produces laccase through the indigenous microorganism in rose slag, does not additionally incorporate carbon source such as white granulated sugar, brown sugar etc., has production cost
Advantage low, fermentation time is short, and it is easy to operate.Phenol oxidase (laccase, peroxide in the environmental protection ferment that the present invention obtains
Enzyme and tyrosinase) activity height, the advantages such as phenolic compound removal rate is high, gives off a strong fragrance, convenient for large-scale promotion application.
Specific embodiment
Invention is further described with reference to embodiments, but the present invention is not limited only to this.
Embodiment 1: a kind of method and application using polyphyll red rose slag preparation environmental protection ferment, it utilizes the red rose of polyphyll
Rare colored slag is raw material, with polyphenol substance therein induction polyphenol degradation enzyme (such as laccase, peroxidase and tyrosinase)
It generates.The polyphyll red rose slag, which refers to, utilizes polyphyll red rose cooking process (condition are as follows: polyphyll red rose fresh flower and water
100 DEG C of boilings are extracted 3-4 hours after the ratio mixing for being 1 to 1 according to weight ratio, and steam is for extracting essential oil, remaining solid
Part is colored slag) colored slag obtained after essential oil is extracted, protein content 7-15% in dry matter is referred in particular to extract after essential oil,
The polyphyll red rose slag of Determination of Polyphenols 7-9%, water content 40-95%.Specifically be made of following steps: (1) environmental protection ferment is sent out
The allotment of ferment matrix: polyphyll red rose slag is adjusted to water content 60wt%, obtains environmental protection ferment fermentation substrate.
(2) it ferments: environmental protection ferment fermentation substrate being fitted into closed container, is fermented at 20 °C, is during which put
Gas 2-3 times.Fermentation time 15 days, obtain environmental protection ferment crude product.
(3) it filters: being filtered environmental protection ferment crude product using 100 meshes, obtain environmental protection ferment product after standing clarification.
Detection method is as follows:
Laccase activity (U) definition: by 1.9 milliliters 50 mM/ls of citrate-phosphate salt buffer (pH 4.0) and 0.5
0.1 milli is added after 60 DEG C keep the temperature 5 minutes in the reaction system of milliliter 10 mM/ls of 2,6- dimethoxy phenol composition
Ferment product stoste is risen, the value added of 470 nanometers absorbances is measured.To aoxidize 1 micromolar 2,6- dimethoxy per minute
Laccase amount required for base phenol is defined as 1 enzyme activity unit (U).
Peroxidase activity (U) definition: with 50 mM/ls, the phosphate buffer (concentration of hydrogen peroxide of pH6.0
For the guaiacol solution for 0.1%) preparing 10 mM/ls, take 2.9 milliliters (60 DEG C keep the temperature 5 minutes) that 0.1 milliliter of ferment is added
Product stoste, 470 nanometers of colorimetrics after mixing, ferment product stoste start timing after being added, 1 light absorption value of every 30 seconds records, with
Straight slope calculates enzyme activity.1 enzyme activity is defined as to aoxidize enzyme amount required for 1 micromolar guaiacol per minute
Unit (U).
Tyrosinase vigor (U) definition: with 50 mM/ls, 50 mM/ls of phosphate buffered saline of pH6.0
Catechol solution, take 2.9 milliliters (60 DEG C keep the temperature 5 minutes) that 0.1 milliliter of ferment product stoste is added, 420 nanometers after mixing
Colorimetric, ferment product stoste start timing after being added, 1 light absorption value of every 30 seconds records calculates enzyme activity with straight slope.With every
Enzyme amount required for 1 micromolar catechol of minute oxidation is defined as 1 enzyme activity unit (U).
The measurement of environmental protection ferment Degradation of Phenol rate, 2,4- Dichlorophenol and the measurement of bisphenol-A degradation rate: above compound is made into
The solution (being dissolved with the phosphate buffer of 50 mM/ls, pH6.0) of 100 mg/litre concentration takes 2.5 milliliters of solution to be added
0.5 milliliter of ferment product stoste is handled 12 hours at 60 DEG C, detects remaining compound concentration using high performance liquid chromatography
And calculate degradation rate.
Polyphenol degrading enzyme (laccase, the peroxidase and tyrosinase) activity and degradation effect of environmental protection ferment are shown in Table 1.
The polyphenol degrading enzymatic activity and degradation effect of 1 environmental protection ferment of table
Embodiment 2: the present embodiment place same as Example 1 repeats no more, the difference is that: it is a kind of to utilize polyphyll red rose
The method and application of flower slag preparation environmental protection ferment, it is raw material using polyphyll red rose slag, with polyphenol substance therein induction
Polyphenol degradation enzyme (such as laccase, peroxidase and tyrosinase) generates.Specifically it is made of following steps:
(1) allotment of environmental protection ferment fermentation substrate: polyphyll red rose slag is adjusted to water content 85wt%, obtains environmental protection ferment
Fermentation substrate.
(2) it ferments: environmental protection ferment fermentation substrate being fitted into closed container, ferments under the conditions of 35 DEG C, during which puts
Gas 2-3 times.Fermentation time 30 days, obtain environmental protection ferment crude product.
(3) it filters: being filtered environmental protection ferment crude product using 100 meshes, obtain environmental protection ferment product after standing clarification.
Polyphenol degrading enzyme (laccase, the peroxidase and tyrosinase) activity of environmental protection ferment described in the present embodiment and degradation
Effect is shown in Table 2.
The polyphenol degrading enzymatic activity and degradation effect of 2 environmental protection ferment of table
Embodiment 3: the present embodiment place same as Example 1 repeats no more, the difference is that: it is a kind of to utilize polyphyll red rose
The method and application of flower slag preparation environmental protection ferment, it is raw material using polyphyll red rose slag, with polyphenol substance therein induction
Polyphenol degradation enzyme (such as laccase, peroxidase and tyrosinase) generates.Specifically it is made of following steps:
(1) allotment of environmental protection ferment fermentation substrate: polyphyll red rose slag is adjusted to water content 95wt%, obtains environmental protection ferment
Fermentation substrate.
(2) it ferments: environmental protection ferment fermentation substrate being fitted into closed container, ferments under the conditions of 40 DEG C, during which puts
Gas 2-3 times.Fermentation time 45 days, obtain environmental protection ferment crude product.
(3) it filters: being filtered environmental protection ferment crude product using 100 meshes, obtain environmental protection ferment product after standing clarification.
Polyphenol degrading enzyme (laccase, the peroxidase and tyrosinase) activity of environmental protection ferment described in the present embodiment and degradation
Effect is shown in Table 3.The polyphenol degrading enzymatic activity and degradation effect of 3 environmental protection ferment of table
Embodiment 4: using the method and application of polyphyll red rose slag preparation environmental protection ferment, it is using polyphyll red rose slag
Raw material is specifically made of following steps:
(1) allotment of ferment fermentation substrate: polyphyll red rose slag is adjusted to water content 85wt%, obtains ferment fermentation substrate.
(2) it ferments: ferment fermentation substrate being fitted into closed container, 5% wine yeast, chestnut brewer yeast, dark red ferment are inoculated with
Female mixed bacteria liquid (preparation method: being 20 parts of glucose by parts by weight, 3 parts of peptone, 15 parts of agar, and 100 parts of mashed potato and water
800 parts are uniformly mixed and obtain culture solutions, by 450 parts of loading triangular flasks of the culture solution, later by 20 parts of wine yeast, and rhodothece rubra
15 parts, the Mixed Microbes that 10 parts of chestnut brewer yeast intervene triangular flask, and shake culture 20 hours, obtains mixed bacteria liquid on 30 DEG C of shaking tables)
37 DEG C ferment 6 days, and 5% lactobacillus bulgaricus, 4% Lactobacillus saki is then added, ferments under the conditions of 37 DEG C, the phase
Between deflation 2-3 times.Fermentation time 30 days, obtain ferment crude product.
(3) it filters: being filtered ferment crude product using 100 meshes, obtain ferment product after standing clarification.
It is detected with detection means same as Example 1 and condition, without polyphenol degrading enzyme ((laccase, peroxide in ferment
Enzyme and tyrosinase)) vigor, and can not degradation of phenol, 2,4- Dichlorophenol and bisphenol-A.
The embodiment is since sugar content is higher in fermentation medium without the reason of polyphenol degrading enzymatic activity, and nutrition is rich
Richness is not easy to generate phenols degrading enzyme using phenolic substances inducing microbial；And major microorganisms are lactic acid bacteria and yeast, are produced more
Phenoloxidase cascade ability is weaker, thus can not generate and active polyphenol degradation enzyme can be detected.
Embodiment 5: using the method and application of polyphyll red rose slag preparation environmental protection ferment, it utilizes polyphyll red rose
Slag is raw material, is specifically made of following steps: (1) allotment of ferment fermentation substrate: according to 10 parts of water, polyphyll red rose slag 3
Part, sugared 1 part of ratio prepare fermentation substrate.
(2) it ferments: ferment fermentation substrate is fitted into closed container, room temperature is closed to stand 3 months, obtains ferment crude product.
(3) it filters: being filtered ferment crude product using 100 meshes, obtain ferment product after standing clarification.
It is detected with detection means same as Example 1 and condition, without polyphenol degrading enzyme (laccase, peroxide in ferment
Enzyme and tyrosinase) vigor, and can not degradation of phenol, 2,4- Dichlorophenol and bisphenol-A.
The embodiment is the microorganism benefit since sugar content is higher in fermentation medium without the reason of polyphenol degrading enzymatic activity
It is full of nutrition, active polyphenol degradation can be detected without utilizing, therefore can not generate phenolic substances as nutritional ingredient
Enzyme.
Embodiment 6: using the method and application of polyphyll red rose slag preparation environmental protection ferment, it utilizes polyphyll red rose
Slag is raw material, is specifically made of following steps:
(1) allotment of ferment fermentation substrate: weighing 1 kilogram of polyphyll red rose slag and 1 kilogram of white granulated sugar, molten with 8 liters of pure water
Polyphyll red rose slag is added after solution white granulated sugar to stir evenly, obtains ferment fermentation substrate, is fitted into spare in light resistant container.
(2) culture of lactic acid bacteria: commercial plant lactobacillus is activated 48 hours for 37 DEG C in MRS culture solution by 2% inoculum concentration,
It is transferred in MRS culture solution and cultivates 48 hours again for 37 DEG C.Wherein MRS cultivates formula of liquid are as follows: and 10 grams of peptone, 5 grams of yeast extract, ox
10 grams of meat medicinal extract, 2 grams of dipotassium hydrogen phosphate, 0.58 gram of epsom salt, four 0.25 gram of water manganese sulfates, 2 grams of dibasic ammonium citrate, second
5 grams, glucose 20.0g of sour sodium, 1 milliliter of Tween 80,1000 milliliters of distilled water, 6.8,121 DEG C of pH sterilize 20 minutes.
(3) lactic acid bacteria microorganism collection: above-mentioned lactic acid bacteria culture solution is centrifuged 10 minutes with 3000 revs/min, discards culture medium
Supernatant is added deionized water suspension thalline precipitating, is centrifuged 10 minutes with 3000 revs/min again, discards supernatant, obtain wet bacterium
Body.
(4) lactobacillus-fermented: weighing 5 grams of lactic acid bacteria wet thallus, is added in the rose raw material mixed, uses cleaning article
It stirs evenly, seals, ferment 10 days under the conditions of 30 DEG C, during which every 24 hours opening containers are sufficiently stirred 1 time.
(5) culture of saccharomycete: commercially available saccharomyces cerevisiae is inoculated into YPD culture solution, 120r revs/min, 25 DEG C, is cultivated 2 days,
Obtain activation fermentation liquid；Activation fermentation liquid is taken, by 10% inoculum concentration 120 revs/min in new YPD culture solution, 25 DEG C of cultures 2
It.YPD cultivates formula of liquid are as follows: 10 grams of yeast extract, 20 grams of peptone, 20 grams of glucose, 1000 milliliters of distilled water, and 121 DEG C of sterilizings
20 minutes.
(6) it collects saccharomycete thallus: by culture solution obtained above, being centrifuged 10 minutes with 3000 revs/min, discard culture
Base supernatant is added deionized water suspension thalline precipitating, is centrifuged 10 minutes with 3000 revs/min again, discards supernatant, obtain wet bacterium
Body.
(7) fermentation raw material supplements: 500 grams of white granulated sugars are weighed, in the system after step (4) lactobacillus-fermented is added, and stirring
Dissolution.
(8) saccharomycetes to make fermentation: weighing 10 grams of saccharomycete wet thallus, is added in the fermentation system of above-mentioned supplement white granulated sugar, makes
It is stirred evenly with cleaning article, seals, ferment 2 days under the conditions of 28 DEG C, during which every 24 hours opening containers are sufficiently stirred 1 time.
(9) it filters: being filtered environmental protection ferment crude product using 100 meshes, obtain ferment product after standing clarification.
It is detected with detection means same as Example 1 and condition, without polyphenol degrading enzyme ((laccase, peroxide in ferment product
Compound enzyme and tyrosinase)) vigor, and can not degradation of phenol, 2,4- Dichlorophenol and bisphenol-A.
The embodiment is the microorganism benefit since sugar content is higher in fermentation medium without the reason of polyphenol degrading enzymatic activity
It is full of nutrition, without being utilized phenolic substances as nutritional ingredient；And major microorganisms bacterial strain be lactic acid bacteria and saccharomycete,
Produce that polyphenol oxidase enzyme system ability is weaker, thus can not generate and active polyphenol degradation enzyme can be detected.
Claims (4)
1. a kind of method using polyphyll red rose slag preparation environmental protection ferment, it is characterized in that it is using polyphyll red rose slag
Raw material is generated with polyphenol substance therein induction polyphenol degradation enzyme, is specifically made of following steps:
(1) allotment of environmental protection ferment fermentation substrate: the polyphyll red rose slag of water content 40-95wt% after squeezing is adjusted to contain
Water 60-90wt% obtains environmental protection ferment fermentation substrate；
(2) it ferments: environmental protection ferment fermentation substrate being fitted into closed container, is fermented under the conditions of 20-40 DEG C, during which deflate
2-3 times；Fermentation time 15-45 days, obtain environmental protection ferment crude product；
(3) it filters: being filtered environmental protection ferment crude product using 100 mesh filter clothes, obtain environmental protection ferment product after standing clarification.
2. the method according to claim 1 using polyphyll red rose slag preparation environmental protection ferment, it is characterized in that described heavy
Valve red rose slag refers to using polyphyll red rose using colored slag obtained after cooking process extraction essential oil.
3. the method according to claim 1 using polyphyll red rose slag preparation environmental protection ferment, it is characterized in that described heavy
Valve red rose slag, which refers to, extracts after essential oil protein content 7-15% in dry matter using cooking process, Determination of Polyphenols 7-9%, aqueous
Measure the polyphyll red rose slag of 40-95%.
4. a kind of application using polyphyll red rose slag as described in claim 1 preparation environmental protection ferment, it is characterized in that being used for
Produce degradation of phenol pollutant environmental protection ferment.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104187731A (en) * | 2014-09-22 | 2014-12-10 | 北海市东雨农业科技有限公司 | Preparation method of environment-friendly enzyme taking lychee exocarp as principal raw material |
| CN104232374A (en) * | 2014-09-26 | 2014-12-24 | 大庆渤基科技开发有限公司 | Environment-friendly ferment soap and preparation method thereof |
| CN105852067A (en) * | 2016-04-29 | 2016-08-17 | 昆明吉庆玫瑰庄园管理有限公司 | Production method of rose enzyme |
| CN106262929A (en) * | 2016-08-30 | 2017-01-04 | 柳州职业技术学院 | A kind of rose enzyme using brown sugar and preparation method thereof |
| CN106333354A (en) * | 2016-08-30 | 2017-01-18 | 柳州职业技术学院 | Rose enzyme and preparation method thereof |
| WO2017186143A1 (en) * | 2016-04-29 | 2017-11-02 | 辽宁晟启昊天生物医药科技有限公司 | Fructooligosaccharide ferment and preparation method therefor |
| WO2017186145A1 (en) * | 2016-04-29 | 2017-11-02 | 辽宁晟启昊天生物医药科技有限公司 | High oxidation resistance active ferment product and preparation method therefor |
| CN108902951A (en) * | 2018-08-28 | 2018-11-30 | 江苏省农业科学院 | A kind of production method of pure-blood ferment rose enzyme |
| CN109529404A (en) * | 2019-01-03 | 2019-03-29 | 山东省科学院能源研究所 | The method for preparing polyphenol extract using polyphyll red rose slag |
-
2019
- 2019-06-18 CN CN201910528319.4A patent/CN110200274A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104187731A (en) * | 2014-09-22 | 2014-12-10 | 北海市东雨农业科技有限公司 | Preparation method of environment-friendly enzyme taking lychee exocarp as principal raw material |
| CN104232374A (en) * | 2014-09-26 | 2014-12-24 | 大庆渤基科技开发有限公司 | Environment-friendly ferment soap and preparation method thereof |
| CN105852067A (en) * | 2016-04-29 | 2016-08-17 | 昆明吉庆玫瑰庄园管理有限公司 | Production method of rose enzyme |
| WO2017186143A1 (en) * | 2016-04-29 | 2017-11-02 | 辽宁晟启昊天生物医药科技有限公司 | Fructooligosaccharide ferment and preparation method therefor |
| WO2017186145A1 (en) * | 2016-04-29 | 2017-11-02 | 辽宁晟启昊天生物医药科技有限公司 | High oxidation resistance active ferment product and preparation method therefor |
| CN106262929A (en) * | 2016-08-30 | 2017-01-04 | 柳州职业技术学院 | A kind of rose enzyme using brown sugar and preparation method thereof |
| CN106333354A (en) * | 2016-08-30 | 2017-01-18 | 柳州职业技术学院 | Rose enzyme and preparation method thereof |
| CN108902951A (en) * | 2018-08-28 | 2018-11-30 | 江苏省农业科学院 | A kind of production method of pure-blood ferment rose enzyme |
| CN109529404A (en) * | 2019-01-03 | 2019-03-29 | 山东省科学院能源研究所 | The method for preparing polyphenol extract using polyphyll red rose slag |
Non-Patent Citations (1)
| Title |
|---|
| 朱玉贤等: "《现代分子生物学》", 31 March 1997, 高等教育出版社 * |
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