CN110200271B - Preparation method of carbon nano material used as health food transfer carrier - Google Patents
Preparation method of carbon nano material used as health food transfer carrier Download PDFInfo
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- CN110200271B CN110200271B CN201910456830.8A CN201910456830A CN110200271B CN 110200271 B CN110200271 B CN 110200271B CN 201910456830 A CN201910456830 A CN 201910456830A CN 110200271 B CN110200271 B CN 110200271B
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/015—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Inorganic Chemistry (AREA)
- Carbon And Carbon Compounds (AREA)
Abstract
The invention discloses a preparation method of a carbon nano material used as a health food transfer carrier, which relates to the technical field of food ingredient nanotechnology, and is characterized in that a single acid treatment method is used for truncating and purifying an original single-walled carbon nano tube to obtain purified SWCNTs; activating carboxyl on the purified SWCNTs, namely adding a long-circulating modifier polyvinylidene, and performing PEG modification to obtain PEGE-modified SWCNTs; and then performing FITC modification on the obtained PEG-modified SWCNTs by adopting a fluorescent agent fluorescein isothiocyanate, and finally dialyzing and drying to obtain the PEG-modified SWCNTs. The reproducibility is good, the cost is low, and the product quality is good; the sample fracture caused by the over-strong oxidability of the mixed acid is avoided; the purified and activated SWCNTs are rich in COOH on the surface, the loading rate of CoQ10 is high, and further functional modification can be carried out; CNTs are modified by PEG, so that the CNTs have a slow release effect.
Description
Technical Field
The invention relates to the technical field of food ingredient nanometer, in particular to a preparation method of a carbon nanometer material used as a health food transfer carrier.
Background
Carbon nanomaterials exist in a variety of forms, among which single-walled carbon nanotubes (SWCNTs) are one-dimensional quantum materials consisting of carbon atoms with a seamless hollow tubular structure, curled with a layer of prismatic carbocyclic ring structure, and also contain ring structures, usually five-membered and seven-membered rings. The end of the single-walled carbon nanotube is of a semi-ring carbon network structure, and the diameter size is 0.6-2.4 nm. The multi-walled carbon nanotubes (MWCNTs) can be formed by two or more than two different concentric curled carbon rings, and the diameter is generally 2.5-10 nm. The bonding of the single-walled carbon nanotube is mainly SP2Mainly hybridized, has larger specific surface area, and a large number of aromatic structures on the surface, so that the organic/inorganic composite material can be combined with various inorganic/organic molecules in a covalent or non-covalent manner, and the special structures enable the organic/inorganic composite material to have wide application prospects in the field of food.
Because of their poor water solubility and toxicity, single-walled carbon nanotubes have limited applications in the food and biomedical fields. The adsorption performance, physiological solubility, biocompatibility and the like of the single-walled carbon nanotube after the functional modification are improved. The modification of the single-walled carbon nanotube mainly comprises two types of covalent modification and non-covalent modification, wherein the non-covalent modification is mainly used for improving the dispersion performance of the single-walled carbon nanotube by intermolecular pi-pi bond accumulation, van der waals force and the like so as to prevent the aggregation of the single-walled carbon nanotube; covalent modification is mainly to modify the surface of the single-walled carbon nanotube by sharing electrons to generate oxygen-containing groups, which is beneficial to the loading of bioactive molecules or drugs.
Coenzyme Q10 (CoQ 10) is a lipid-soluble steroid that prevents the decrease of ATP anabolism and activates respiratory function of cells. When CoQ10 is deficient in vivo, health conditions can decline, especially in patients with cardiovascular and cerebrovascular diseases, diseases associated with immune function, and cancer. Therefore, CoQ10 can be used as a potential functional health food, and specific people supplement and take exogenous CoQ10, which has important significance for maintaining cardiovascular and cerebrovascular health and preventing aging.
The absorption rate of CoQ10 in the intestinal tract is very slow, because CoQ10 has a large molecular weight and is not readily soluble in water, and therefore has a low bioavailability when taken directly orally. Meanwhile, CoQ10 is easily oxidized by air when exposed to air and easily decomposed by light, so that the stability of the product in conventional preparations such as capsules, tablets and injections is poor, and the product is difficult to store for a long time, thereby affecting the quality and the using effect of the product.
At present, how to effectively improve stability and bioavailability of CoQ10 became a research focus, and new formulations of CoQ10 including solid dispersions, cyclodextrin inclusion compounds, emulsions, microcapsules, liposomes, and the like have been developed. However, these preparations still have the disadvantages of poor stability, poor absorption effect, etc. to some extent. The CNTs as a novel carrier of food nutrient components have obvious advantages in the aspects of improving bioavailability and targeting, protecting and slowly releasing embedded objects in the digestive process of gastrointestinal tracts and the like. The SWCNTs loaded CoQ10 can effectively enhance the stability of CoQ10, prevent the CoQ10 from oxidative degradation and promote absorption. Meanwhile, the performance of the carrier can be further improved by taking the high molecular material polyethylene glycol (PEG) as a modifier.
Disclosure of Invention
Aiming at the defects and shortcomings of the prior art, the invention provides a preparation method of a carbon nano material serving as a health-care food transfer carrier, which is reasonable in design, and has the advantages of good reproducibility, low cost and good product quality; the sample fracture caused by the over-strong oxidability of the mixed acid is avoided; the purified and activated SWCNTs are rich in COOH on the surface, the loading rate of CoQ10 is high, and further functional modification can be carried out; CNTs are modified by PEG, so that the CNTs have a slow release effect.
In order to achieve the purpose, the invention adopts the following technical scheme: the method comprises the following steps:
1. truncating and purifying original single-walled carbon nanotubes (SWCNTs) by using a single acid treatment method to obtain purified SWCNTs;
2. activating carboxyl on the purified SWCNTs, namely adding long-circulating modifier Polyvinylidene (PEG) for PEG modification to obtain SWCNTs modified by PEGE; and then performing FITC (fluorescein isothiocyanate) modification on the obtained PEG-modified SWCNTs by using a fluorescent agent FITC, and finally dialyzing and drying to obtain the FITC-modified SWCNTs.
Further, loading CoQ10 on the SWCNTs modified by FITC, the SWCNTs modified by PEGE and the SWCNTs modified by FITC by a mixing and stirring method; establishing an HPLC method for measuring CoQ10, measuring the content of CoQ10 in an in-vitro release experiment, and calculating the drug loading rate and the encapsulation rate of the carbon nano material loaded with CoQ 10.
Further, in the step 1, the particle size of the purified SWCNTs is between 120-600 nm.
Further, in the step 1, SWCNTs are purified by the following steps: adding single acid solution into the original SWCNTs, condensing and refluxing at high temperature, filtering after cooling, repeatedly washing and drying in vacuum.
Further, the health food is polysaccharide or monosaccharide, vitamin, glutathione, octacosanol and the like.
Further, the health food is selected from any one of a free radical scavenger, vitamin B and oligosaccharide.
Further, the volume-to-mass ratio of the acid solution of the single acid treatment method in the step 1 to the original SWCNT is 1:1 (ml: mg).
Further, in the step 2, in the process of performing PEG modification, the mass ratio of the purified SWCNTs (namely SWCNTs-COOH) to the PEG is 1:1-1:2, and in the process of performing FITC, the mass ratio of the SWCNTs (namely SWCNTs-PEG) to the FITC is 50:1-10: 1.
Further, the mass ratio of the carbon nano material to the health food is 1:2-2: 1.
After the method is adopted, the invention has the beneficial effects that:
1. the original SWCNTs are purified by acid treatment, and the method has good reproducibility, low cost and good product quality;
2. using a single acid (concentrated HNO)3) The treatment avoids mixed acid (such as concentrated sulfuric acid and concentrated nitric acid, or concentrated sulfuric acid and H)2O2) Sample fracture due to too strong oxidation;
3. the purified and activated SWCNTs are rich in COOH on the surface, the loading rate of CoQ10 is high, and further functional modification can be carried out;
4. and modifying the acidified SWCNTs by using PEG (polyethylene glycol) so as to ensure that the SWCNTs have a slow release effect.
Description of the drawings:
FIG. 1 is a diagram of the original SWCNTs (blank sample), SWCNTs-COOH, SWCNTs-PEG, and,
Transmission electron microscopy of SWCNTs-PEG-FITC.
FIG. 2 is a scanning electron micrograph of pristine SWCNTs (blank sample), SWCNTs-COOH, SWCNTs-PEG.
FIG. 3 shows the IR absorption spectra of pristine SWCNTs (blank sample), SWCNTs-COOH, SWCNTs-PEG, and SWCNTs-PEG-FITC.
FIG. 4 is a diagram of the original SWCNTs (blank sample), SWCNTs-COOH, SWCNTs-PEG, and,
XRD profile of SWCNTs-PEG-FITC.
FIG. 5 is a diagram of the original SWCNTs (blank sample), SWCNTs-COOH, SWCNTs-PEG, and,
Raman spectrum of SWCNTs-PEG-FITC.
FIG. 6 is a representation of the original SWCNTs (blank sample), SWCNTs-PEG-FITC
DSC graph of (a).
FIG. 7 is a graph of the in vitro release of SWCNTs-COOH loaded with CoQ10, and SWCNTs-PEG loaded with CoQ 10.
FIG. 8 is a diagram of the original SWCNTs (blank sample), SWCNTs-COOH, SWCNTs-PEG, and,
The particle size and Zeta potential of SWCNTs-PEG-FITC are shown in the table.
FIG. 9 is a table showing the results of drug loading and encapsulation efficiency measurements of SWCNTs-COOH loaded with CoQ10 and SWCNTs-PEG loaded with CoQ 10.
The specific implementation mode is as follows:
the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The specific implementation mode adopts the following technical scheme:
1. purification (single acid treatment): accurately weighing 100mg of original SWCNTs in a flask, and measuring 100mL of concentrated HNO3Adding the SWCNTs into a round-bottom flask along the bottle mouth of the round-bottom flask, and simultaneously flushing the SWCNTs attached to the wall; performing ultrasonic treatment in an ultrasonic cleaner for 3 hours to uniformly disperse the SWCNTs; adding magneton, and placing in
Stirring in an oil bath pan, adjusting the temperature to 80 ℃, and installing a condensing tube for condensing and refluxing for 24 hours; after the reaction is finished, the reaction solution is added,
diluting with deionized water, taking out magnetons, and cooling and standing the round-bottom flask; performing suction filtration, dispersing the obtained black solid in deionized water, centrifuging at 10000rpm for 10min, removing the upper liquid, and repeating the step until the carbon nano tube is washed to be neutral; placing the sample in a drying oven at 50 ℃ and drying to constant weight to obtain purified SWCNTs, and marking the purified SWCNTs as SWCNTs-COOH for later use;
2. PEG modification: 200mg of SWCNTs-COOH, 250mg of N-hydroxysuccinimide (NHS) and 200mg of carbodiimide (EDC. HCl) were precisely weighed, 100mL of deionized water was added, and the mixture was rinsed
Washing off the material attached to the bottle wall, and controlling the solution concentration at the pH of 5-7; ultrasonic treating for 30min, adding 250mg
NH of (2)2-PEG2000-NH2Mixing uniformly; adding magnetons, and violently stirring in an oil bath kettle at 45 ℃ for 24 hours; taking out the magnetons after the reaction is finished, carrying out suction filtration, dispersing the obtained black solid in a small amount of deionized water, and dialyzing for 48 hours; drying the dialyzed sample to constant weight to obtain SWCNTs modified by PEG, and marking as SWCNTs-PEG;
3. FITC modification: 100mg of SWCNTs-PEG is precisely weighed, and a proper amount of NaHCO with pH of 9.6 is added3-Na2CO3Buffering the solution and washing away the material attached to the bottle wall; performing ultrasonic treatment for 30min, adding magneton, and stirring in oil bath at normal temperature in dark place; 3mg of FITC, dissolved in 3g of dimethyl sulfoxide (DMSO); dropwise adding the solution into CNTs-PEG dispersion liquid, and stirring for 24 hours at normal temperature; taking out the magnetons after the reaction is finished, and performing suction filtration; collecting a sample, adding a small amount of deionized water, and dialyzing for 48 hours; and drying the dialyzed sample to constant weight to obtain SWCNTs modified by FITC, and marking as SWCNTs-PEG-FITC.
4. Determination of CoQ10 by HPLC method:
4.1, chromatographic conditions:
a chromatographic column: c18 column (4.6 mm. times.200 mm, 5 μm), mobile phase: methanol-n-hexane (4: 1), column temperature: 25 ℃, flow rate: 1.0mL/min, detection wavelength: 275nm, sample size: 10 mu L of the solution;
4.2, establishing a method for measuring CoQ10, and preparing for measuring the content of CoQ10 and calculating the encapsulation efficiency and drug loading rate in an in-vitro release experiment.
4.3, load CoQ 10: 100mg of CoQ10 was dissolved in absolute ethanol, and 100mg of CoQ10 was added
SWCNTs-PEG-FITC is dispersed in the solution; then adding a proper amount of Phosphate Buffer Solution (PBS) with pH7.4 dropwise, and carrying out ultrasonic treatment for 30 min; adding magnetons, and vigorously stirring in an oil bath kettle at 37 ℃ for 24 hours to enable CoQ10 to be adsorbed on SWCNTs-PEG-FITC; centrifuging, collecting precipitate, washing with PBS solution for several times, drying to constant weight,
obtaining CNTs-PEG-FITC/CoQ 10; loading CoQ10 to original SWCNTs and SWCNTs-COOH in the same way
And SWCNTs-PEG to obtain three samples, namely SWCNTs/CoQ10, SWCNTs-COOH/CoQ 10 and SWCNTs-PEG/CoQ 10;
5. the particle size, PDI and Zeta potential of each carrier are measured, and transmission electron microscope and scanning electron microscope observation are carried out, namely, the original SWCNTs (blank sample), SWCNTs-COOH, SWCNTs-PEG and SWCNTs-PEG-FITC take deionized water as a dispersion medium, and the particle size, PDI and Zeta potential are measured by a Malvern nanometer particle size analyzer. The measurement observations are as follows:
referring to fig. 8, the original SWCNTs have a large particle size, and after purification, the particle size becomes small, and the Zeta potential of the purified SWCNTs (i.e., SWCNTs-COOH) reaches-41.3 mV, which has good stability; the Zeta potentials of SWCNTs-PEG and SWCNTs-PEG-FITC are respectively about-25.1 mV and-27.5 mV, untreated SWCNTs (i.e. original SWCNTs) are only-2.14 mV, the stability is extremely poor, the raw SWCNTs are easy to rapidly agglomerate, meanwhile, the dispersibility of the original SWCNTs is poor, and the purified and modified SWCNTs (i.e. SWCNTs-COOH, SWCNTs-PEG and SWCNTs-PEG-FITC) have better dispersibility.
Referring to FIG. 1, where A is pristine SWCNTs, B is SWCNTs-COOH, C is SWCNTs-PEG, and D is SWCNTs-PEG-FITC; the original SWCNTs are long, poor in dispersity and easy to aggregate; the SWCNTs-COOH is shorter, the surface is smoother, the dispersibility is better than that of the original SWCNTs, and the residual amorphous carbon and metal catalyst on the surface are basically removed; as for SWCNTs-PEG and SWCNTs-PEG-FITC, it is obvious that a chain-like substance is coated on the surface.
Referring to FIG. 2, where A is raw SWCNTs, B is SWCNTs-COOH, C is SWCNTs-PEG, raw SWCNTs are elongated filaments intertwined to form a network, the acid treated SWCNTs-COOH is shorter than before treatment, and due to the concentrated HNO3The strong oxidizing property of the SWCNTs enables the surface appearance of the SWCNTs to be changed; the surface of SWCNTs-PEG has attachments.
6. The carriers are characterized by infrared spectrum analysis, X-ray diffraction (XRD), Raman spectrum, Differential Scanning Calorimetry (DSC) and the like, and the characterization results are as follows:
referring to FIG. 3, the scanning wave number range is 500-4000 cm-1, the X-axis represents the transmittance (%) and the Y-axis represents the wave number in cm-1. The original SWCNTs (spectrum of Blank sample in the figure) have no more obvious characteristic peak, while the purified SWCNTs (SWC in the figure)The spectrum of NTs-COOH), the spectrum of PEG-modified SWCNTs (SWCNTs-PEG in the figure), and the spectrum of FITC-modified SWCNTs (SWCNTs-PEG-FITC in the figure) were all 3430cm-1、1630cm-1Has an absorption peak at 3430cm-1Shows the stretching vibration peak of the O-H bond, and 1630cm-1The peak of stretching vibration of C = O bond in the carboxyl group is shown. After acid treatment, at 2930cm-1、2140cm-1、1170cm-1Characteristic peaks appear, which correspond to the stretching vibration and deformation vibration absorption peaks of C-H, C ≡ C and C-C bonds respectively, which shows that after acid treatment, the SWCNTs have increased carbon hybridized with sp3 on the surface and increased defects. In the figure, PEG modified SWCNTs and FITC modified SWCNTs have absorption peaks at the corresponding positions of O-H, C = O, but the peak intensity is much weaker than that of SWCNTs-COOH, and the fact that the modified SWCNTs also have oxygen-containing groups such as carboxyl, hydroxyl and carbonyl on the surfaces is shown that the modification process is covalent modification, so that the oxygen-containing groups are reduced.
Referring to FIG. 4, the characteristic peaks of the original SWCNTs are at 26 ℃ and 44 ℃, and the SWCNTs-COOH and SWCNTs-PEG-FITC have the same diffraction peaks at the two positions, which indicates that the structure of the SWCNTs is not seriously damaged in the reaction and is relatively intact. The characteristic peaks of the purified SWCNTs, the PEG-modified SWCNTs and the FITC-modified SWCNTs are more obvious than those of the original SWCNTs.
Referring to FIG. 5, the original SWCNTs were at 1330cm-1And 1587cm-1There are two characteristic peaks. 1330cm-1The characteristic peak represents a Raman D band caused by defects and disordered structures of SWCNTs; and 1587cm-1Here, the cleavage G band appears after the formation of SWCNTs, and is a reaction of order degree. The ratio of the intensity of the D band to the intensity of the G band is ID/IG, and can be used for comparing the defect degree on the carbon nano tube, and the higher the value of ID/IG is, the more defects are on SWCNTs. The ID/IG ratio of the original SWCNTs was 0.214, indicating that the structure was relatively intact and the sample was purer, but the ratio was lower and the defects were too few. Acid treated SWCNTs ID/IG
The ratio increased to 0.361, indicating that SWCNTs had more defects with acid oxidation, and that
Indicating that the purified SWCNTs are much more surface functionalized than the original SWCNTs. The ID/IG ratio of the SWCNTs modified by PEG is 0.161, which shows that carbon atoms on the SWCNTs also have certain reaction in the modification process, and the D peak intensity of the modified SWCNTs is obviously weakened because the modified SWCNTs are in a loose structure, so that the D peak intensity is weakened. The ID/IG ratio of SWCNTs modified by FITC is 0.227, which shows that the defect degree of SWCNTs-PEG is improved after FITC modification.
Referring to fig. 6, the original SWCNTs did not have a significant peak weight loss; the PEG modified SWCNTs have two weight loss peaks at 106.1 ℃ and 296.3 ℃, and the FITC modified SWCNTs have two weight loss peaks at 137.5 ℃ and 345.1 ℃. The first peak is the weight loss peak of water or solvent absorbed by SWCNTs, and the second peak is related to the PEG skeleton fracture of SWCNTs surface modification and the carboxyl decomposition of surface unreacted.
The comprehensive characterization results show that compared with the original SWCNTs, the physical and chemical properties of the SWCNTs are obviously changed after acid treatment and functional modification, and the purification and surface modification of the SWCNTs are proved
The expected effect is achieved.
7. And (3) determining the drug loading and the encapsulation efficiency:
centrifuging SWCNTs-COOH/CoQ 10 and SWCNTs-PEG/CoQ10, measuring the volume of the supernatant, carrying out sample injection measurement, substituting the peak area into a standard curve to obtain the content of CoQ10 in the supernatant, and substituting the content into a formula to calculate:
drug loading = drug amount (entrapped) per carrier amount
Entrapment rate = amount of drug (package load)/total amount of drug;
the determination result is shown in figure 9, and the result shows that both SWCNTs-COOH and SWCNTs-PEG can effectively load CoQ10, and the drug-loading performance of the SWCNTs (namely SWCNTs-PEG) modified by PEG is obviously improved.
8. Determination of in vitro release:
taking 100mL of release medium, placing the release medium in a conical flask, respectively taking 20mg of CoQ 10-loaded SWCNTs-COOH and SWCNTs-PEG to be respectively dispersed in 10 mL of release medium, adding the release medium into a dialysis bag, sealing two ends of the dialysis bag, placing the dialysis bag in the release medium, placing the conical flask in an oscillator, starting oscillation at 37 ℃, wherein the oscillation rate is 160 r/min. Sampling for 1, 2, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96 and 120 hours respectively, sampling 2mL each time, carrying out sample injection and determination, and supplementing an equivalent medium in time;
selection of release medium: since CoQ10 is insoluble in water and thus cannot be directly used as a release medium by PBS solution or deionized water, the method selects to dissolve CoQ10 by absolute ethyl alcohol and selects absolute ethyl alcohol-PBS
(volume ratio 4: 1) mixed solution as release medium. HPLC method is used to determine the drug content in the release solution at different time points. After 120h, multiple measurements show that the concentration of the obtained CoQ10 tends to be stable, and the concentration of CoQ10 at the moment can be considered as the concentration of SWCNTs after being completely released. The percent release was calculated according to the formula: qn=CnVo+∑CiVi(i = 0-n-1); percent released (%) = Q/W100%; the time is plotted on the abscissa and the drug release rate is plotted on the ordinate.
Referring to FIG. 7, the result shows that the release rate of SWCNTs-COOH is fast within 24h, the release rate has reached 81.01% within 24h, and the release rate becomes remarkably slow and gradually stabilizes after 36 h; while the release rate of SWCNTs-PEG is relatively slow in 48h, and the release rate gradually becomes stable after 72 h. Therefore, the functional modification of PEG can realize the slow release of the SWCNTs to the CoQ10, and the expected effect is achieved.
After the method is adopted, the beneficial effects of the specific embodiment are as follows:
1. the original SWCNTs are purified by acid treatment, and the method has good reproducibility, low cost and good product quality;
2. the single acid (concentrated HNO 3) is used for treatment, so that the sample fracture caused by over strong oxidizability of mixed acid (such as concentrated sulfuric acid plus concentrated nitric acid or concentrated sulfuric acid plus H2O 2) is avoided;
3. the purified and activated SWCNTs are rich in COOH on the surface, the loading rate of CoQ10 is high, and further functional modification can be carried out;
4. and modifying the acidified SWCNTs by using PEG (polyethylene glycol) so as to ensure that the SWCNTs have a slow release effect.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (4)
1. A preparation method of carbon nano-materials used as a health food transfer carrier is characterized in that: the health food is polysaccharide or monosaccharide, vitamin, glutathione and octacosanol, and comprises the following steps:
(1) adding concentrated HNO to raw SWCNTs using a single acid treatment process3Condensing and refluxing at high temperature, cooling, filtering, repeatedly washing and vacuum drying, and truncating and purifying the original single-walled carbon nanotube to obtain purified SWCNTs, wherein the particle size of the purified SWCNTs is between 120-600 nm; concentrated HNO3The volume mass ratio of the SWCNT to the original SWCNT is 1: 1;
(2) activating carboxyl on the purified SWCNTs, namely adding long-cycle modifier polyvinylidene, and performing PEG modification to obtain the SWCNTs modified by PEGE; then, performing FITC modification on the obtained PEG-modified SWCNTs by adopting a fluorescent agent fluorescein isothiocyanate, and finally dialyzing and drying to obtain the PEG-modified SWCNTs; in the PEG modification process, the mass ratio of the purified SWCNTs to the PEG is 1:1-1:2, and in the FITC modification process, the mass ratio of the SWCNTs to the FITC is 50:1-10: 1.
2. The method for preparing a carbon nanomaterial as a health food delivery vehicle according to claim 1, wherein: loading CoQ10 on the original SWCNTs, the purified SWCNTs, the PEGE modified SWCNTs and the FITC modified SWCNTs respectively by a mixing and stirring method; establishing an HPLC method for measuring CoQ10, measuring the content of CoQ10 in an in-vitro release experiment, and calculating the drug loading rate and the encapsulation rate of the carbon nano material loaded with CoQ 10.
3. The method for preparing a carbon nanomaterial as a health food delivery vehicle according to claim 1, wherein: the health food is selected from vitamin B and oligosaccharide.
4. The method for preparing a carbon nanomaterial as a health food delivery vehicle according to claim 1, wherein: the mass ratio of the carbon nano material to the health food is 1:2-2: 1.
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