CN110200190A - Harmful microbe spice composition in a kind of inhibition food - Google Patents

Harmful microbe spice composition in a kind of inhibition food Download PDF

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Publication number
CN110200190A
CN110200190A CN201910649333.XA CN201910649333A CN110200190A CN 110200190 A CN110200190 A CN 110200190A CN 201910649333 A CN201910649333 A CN 201910649333A CN 110200190 A CN110200190 A CN 110200190A
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CN
China
Prior art keywords
food
spice composition
harmful microbe
linalool
eugenol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910649333.XA
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Chinese (zh)
Inventor
林雅慧
高裕锋
陈海宁
樊丽娜
邓倩南
苏俊婷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Institute of Bioengineering Guangzhou Cane Sugar Industry Research Institute
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Guangdong Institute of Bioengineering Guangzhou Cane Sugar Industry Research Institute
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Application filed by Guangdong Institute of Bioengineering Guangzhou Cane Sugar Industry Research Institute filed Critical Guangdong Institute of Bioengineering Guangzhou Cane Sugar Industry Research Institute
Priority to CN201910649333.XA priority Critical patent/CN110200190A/en
Publication of CN110200190A publication Critical patent/CN110200190A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3409Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor
    • A23L3/3445Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor in a controlled atmosphere comprising other gases in addition to CO2, N2, O2 or H2O
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to field of food safety, more particularly to harmful microbe spice composition in a kind of inhibition food, it is characterised in that it includes following components in percentage by weight, citral 5%~50%, cinnamic acid 10%~50%, linalool 5%~50%, eugenol 1%~40%;The present invention has the characteristics that safe, natural, nontoxic, there is inhibiting effect to food-borne pathogens such as staphylococcus aureus, escherichia coli, salmonella, Shigella, vibrio parahaemolytious, Listeria monocytogenes, bacillus cereus, can effectively prevent food apoilage, Shelf-life.

Description

Harmful microbe spice composition in a kind of inhibition food
Technical field
The present invention relates to field of food safety, and in particular to harmful microbe spice composition in a kind of inhibition food.
Background technique
Status of the anti-corrosive fresh-keeping in modern food industry is more and more important, the whole world every year due to corruption caused by food Loss up to 20%, causes huge economic loss, wherein putrid and deteriorated the most serious caused by microorganism, and many corruption Microorganism is also food-borne pathogenic microorganism simultaneously.Food industry at present is using processing method (hot-working, cold working, height Pressure, microwave, irradiation etc.) with the mode of preservative synergy to carry out food antiseptic fresh-keeping.Being become using antisepsis antistaling agent is prevented Food apoilage, Shelf-life and a kind of means the most convenient, economical and effective for ensuring Safety of Food Quality, it is special It is not that the advantages that chemical synthesis preservative is easy to use with its, additive amount is small, low in cost, significant effect is widely applied. But the genotoxic potential of chemical synthesis preservative (carcinogenicity, teratogenesis, mutagenesis) makes it ensure the same of food safety in anti-corrosion When bring new food-safety problem.Therefore, efficient, safe and stable new food preservative is developed to be of great significance.
Fragrance essential oil have the characteristics that it is safe, natural, nontoxic, research fragrance essential oil as preservative be it is domestic and international in recent years Open up the research hotspot of novel foodstuff anti-corrosion frontier.In recent years it has been demonstrate,proved by numerous studies in relation to the effect of fragrance essential oil anti-corrosive antibacterial It is real, safe and efficient, economic anti-corrosive antibacterial substance is screened out from it as food preservative, is had broad application prospects.
Summary of the invention
Based on the above issues, the purpose of the present invention is to provide a kind of perfume (or spice) of harmful microorganism growth and breeding in inhibition food Feed composition provides a kind of efficient, safe, natural, nontoxic antiseptic and antibiotic agent for food, medicine.
The purpose of the invention is achieved by the following technical solution:
Harmful microbe spice composition in a kind of inhibition food, the component including following weight ratio, citral 5%~ 50%, cinnamic acid 10%~50%, linalool 5%~50%, eugenol 1%~40%.
Preferably, harmful microbe spice composition in a kind of inhibition food, the component including following weight ratio, lemon Aldehyde 10%~40%, cinnamic acid 15%~40%, linalool 10%~40%, eugenol 5%~30%.
Preferably, citral, cinnamic acid, linalool, eugenol derive from plants essential oil rich in these fragrance, natural Equivalent flavorant.
Preferably, it is a kind of inhibit food in harmful microbe spice composition application method are as follows: by above-mentioned citral, Cinnamic acid, linalool, eugenol use after being mixed directly in proportion, or with solvent dissolved dilution, addition carrier after use.
Preferably, spice composition is directly made an addition in food when use, or be placed in food packaging, pass through volatilization Gas phase reaches bacteriostasis.
Preferably, the solvent is water, propylene glycol, edible alcohol, grease, the one or more of them in Tween-80.
Preferably, the carrier is starch, colloid, the one or more of them in dextrin.
Above-mentioned technical proposal has the advantages that
1. the combinations of perfumes mixed using citral, cinnamic acid, linalool, eugenol by above-mentioned weight percent Object, relatively chemical synthetic preservative have the characteristics that safe, natural, nontoxic.
2. the synergistic function between fragrance component enables spice composition effectively to inhibit the growth of microorganism, especially right Staphylococcus aureus, escherichia coli, salmonella, Shigella, vibrio parahaemolytious, Listeria monocytogenes, waxy bud Spore bacillus has strong inhibitory effect, can effectively prevent food apoilage, Shelf-life.
3. the usage amount of spice composition is smaller, flavour of food products will not be significantly affected.
Specific embodiment
For the ease of the understanding of those skilled in the art, below with reference to embodiment, the present invention is further illustrated, real The content for applying example is not limitation of the invention.
Embodiment one
Harmful microbe spice composition in a kind of inhibition food, the component including following weight ratio, citral 30%, Cinnamic acid 30%, linalool 15%, eugenol 25%.
It takes above-mentioned mono-isolate to mix in closed glass container according to the above ratio, obtains spice composition.
Embodiment two
Harmful microbe spice composition in a kind of inhibition food, the component including following weight ratio, citral 25%, Cinnamic acid 35%, linalool 25%, eugenol 15%.
It takes above-mentioned mono-isolate to mix in closed glass container according to the above ratio, propylene glycol dilution is added, wherein fragrant Feed composition 5wt%, propylene glycol 95wt%, the spice composition after being diluted.
Embodiment three
Harmful microbe spice composition in a kind of inhibition food, the component including following weight ratio, citral 10%, Cinnamic acid 35%, linalool 35%, eugenol 20%.
Take above-mentioned mono-isolate to mix in closed glass container according to the above ratio, be added blending gellan gum, distilled water, Tween-80 obtains the spice composition of gel-type.
Test case one
Sterile working pipettes 15mL nutrient agar to culture dish, after culture medium solidification, opens culture ware lid, will Culture medium exposure is inoculated with 2h naturally in air.
The centre in culture dish is added dropwise in the spice composition that Example one obtains, and separately takes one group of culture dish addition phase The aqua sterilisa of same volume, as blank group;Culture ware lid is covered, is sealed, is cultivated at 37 DEG C for 24 hours, observes bacterial growth situation.
The amount of added spice composition calculates addition with the complete volatile concentrations 0.05 ‰ in space and (volatilizees completely dense in space Degree=addition concentration of perfume × additive amount/spatial volume).
Test case two
Sterile working pipettes 15mL nutrient agar to culture dish, after culture medium solidification, opens culture ware lid, will Culture medium exposure is inoculated with 2h naturally in air.
The centre in culture dish is added dropwise in the spice composition that Example one obtains, and separately takes one group of culture dish addition phase The aqua sterilisa of same volume, as blank group;Culture ware lid is covered, is sealed, 3d is cultivated at 25 DEG C, observes fungus growth situation.
The amount of added spice composition calculates addition with the complete volatile concentrations 0.05 ‰ in space and (volatilizees completely dense in space Degree=addition concentration of perfume × additive amount/spatial volume).
Test case three
Sterile working pipettes 15mL nutrient agar to culture dish, after culture medium solidification, opens culture ware lid, will Culture medium exposure is inoculated with 2h naturally in air.
A small pieces aseptic filter paper piece is pasted in culture ware lid medial center, the combinations of perfumes after the dilution that Example two obtains Object is added dropwise in the aqua sterilisa on filter paper, separately taking the aseptic filter paper piece in one group of culture ware lid to add same volume, as blank Group;Culture ware lid is covered, is cultivated at 37 DEG C for 24 hours, observes bacterial growth situation.
The amount of added spice composition calculates addition with the complete volatile concentrations 0.05 ‰ in space and (volatilizees completely dense in space Degree=addition concentration of perfume × additive amount/spatial volume).
Test case four
Sterile working pipettes 15mL nutrient agar to culture dish, after culture medium solidification, opens culture ware lid, will Culture medium exposure is inoculated with 2h naturally in air.
Paste a small pieces aseptic filter paper piece in culture ware lid medial center, spice composition that Example two obtains be added dropwise in On filter paper, the aseptic filter paper piece in one group of culture ware lid is separately taken to add the aqua sterilisa of same volume, as blank group;Cover training Ware lid is supported, 3d is cultivated in sealing at 25 DEG C, observes fungus growth situation.
The amount of added spice composition calculates addition with the complete volatile concentrations 0.05 ‰ in space and (volatilizees completely dense in space Degree=addition concentration of perfume × additive amount/spatial volume).
1 test case one to four of table observes comparison table
Wherein, bacteriostasis rate=(blank group clump count-test group clump count)/blank group clump count
Test case five
Staphylococcus aureus, escherichia coli, salmonella, Shigella, vibrio parahaemolytious, Dan Zeng are cultivated respectively Listeria, bacillus cereus, it is 10 that cell concentration, which is made,6-107The uniform bacteria suspension or spore suspension of CFU/mL is added dropwise The above-mentioned suspension of 1mL is coated with so uniform that contain bacterium plate in nutrient agar.
The 15 μ L of spice composition of Example one is added drop-wise on sterile 10mmol/L circle filter paper, aseptically Filter paper is attached to the center of bacterium plating medium, bacterium plate is then placed in 37 DEG C and is cultivated 1 day, the size of inhibition zone is measured; The bacterium plate for separately taking the same terms, is added 5% potassium sorbate aqueous solution of same volume, as control experiment, experimental result is such as Shown in table 2.
2 antibacterial circle diameter table of comparisons unit of table: mm
Strain Embodiment one 5% potassium sorbate
Staphylococcus aureus 44.1 35.2
Escherichia coli 40.6 32.8
Salmonella 41.2 35.6
Shigella 42.5 31.9
Vibrio parahaemolytious 43.6 32.5
Listeria monocytogenes 40.8 23.1
Bacillus cereus 41.9 29.5
Test case six
It takes and is put in respectively with two, a batch of bread in the glassware of two sealings, wherein the ware of a glassware Lid inside is stained with the gel-type spice composition of the preparation of embodiment three, and another group is used as blank control, is sealed.
(space is complete with the complete calculating of volatile concentrations 0.05 ‰ addition in space for the amount of added gel-type spice composition Volatile concentrations=addition concentration of perfume × additive amount/spatial volume).
The above-mentioned glassware equipped with bread is placed in 25 DEG C, is placed five days in the environment of relative humidity 85%, viewing surface Packet variation.
3 test case six of table observes comparison table
Test group Blank group
Situation It is unchanged There are peculiar smell, mildew
The above test case shows that this spice composition has strong inhibiting effect to microorganism, can effectively prevent food spoilage Rotten, Shelf-life.
Above-described embodiment is the preferable implementation of the present invention, and in addition to this, the present invention can be existing with other way, not Be detached from present inventive concept under the premise of it is any obviously replace it is within the scope of the present invention.

Claims (7)

1. harmful microbe spice composition in a kind of inhibition food, which is characterized in that the component including following weight ratio, lemon Lemon aldehyde 5%~50%, cinnamic acid 10%~50%, linalool 5%~50%, eugenol 1%~40%.
2. inhibiting harmful microbe spice composition in food according to claim 1, which is characterized in that including following heavy The component of amount ratio, citral 10%~40%, cinnamic acid 15%~40%, linalool 10%~40%, eugenol 5%~ 30%.
3. harmful microbe spice composition in -2 inhibition food according to claim 1, which is characterized in that citral, Cinnamic acid, linalool, eugenol derive from the flavorant of plants essential oil rich in these fragrance, natural equivalent.
4. harmful microbe spice composition in inhibition food according to claim 1 to 2, which is characterized in that it is used Method are as follows: used after being mixed directly each component by claim 1-2 any proportion, or with solvent dissolved dilution, addition It is used after carrier.
5. application method according to claim 4, which is characterized in that spice composition is directly made an addition to food when use In, or be placed in food packaging.
6. application method according to claim 4, it is characterised in that: the solvent is water, propylene glycol, edible alcohol, oil One or more of them in rouge, Tween-80.
7. application method according to claim 4, it is characterised in that: the carrier is starch, colloid, its in dextrin In it is one or more.
CN201910649333.XA 2019-07-18 2019-07-18 Harmful microbe spice composition in a kind of inhibition food Pending CN110200190A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110897005A (en) * 2019-11-15 2020-03-24 华南理工大学 Method for sterilizing gram-negative pathogenic bacteria in milk

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101411538A (en) * 2008-11-14 2009-04-22 广东工业大学 Spice composition for inhibiting food spoilage microorganisms
CN102150922A (en) * 2011-01-24 2011-08-17 广东工业大学 Plant essential oil composition for killing harmful bacteria of uncooked food
CN104757551A (en) * 2015-04-29 2015-07-08 无限极(中国)有限公司 Plant extract composite for restraining microorganisms and application thereof
CN106543743A (en) * 2016-10-21 2017-03-29 北京化工大学 A kind of hydrolysate of the collagen containing linalool/edible antibacterial film of chitosan quaternary ammonium salt and preparation method thereof
CN107006593A (en) * 2017-03-10 2017-08-04 浙江省农业科学院 A kind of fruits and vegetables antibacterial shock absorbing foam
CN108024528A (en) * 2015-09-30 2018-05-11 包装运输及物流技术研究所 Application including citral, hexanal and linalool antimicrobial compositions as active component and in packaging micro Process fruit or vegetables

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101411538A (en) * 2008-11-14 2009-04-22 广东工业大学 Spice composition for inhibiting food spoilage microorganisms
CN102150922A (en) * 2011-01-24 2011-08-17 广东工业大学 Plant essential oil composition for killing harmful bacteria of uncooked food
CN104757551A (en) * 2015-04-29 2015-07-08 无限极(中国)有限公司 Plant extract composite for restraining microorganisms and application thereof
CN108024528A (en) * 2015-09-30 2018-05-11 包装运输及物流技术研究所 Application including citral, hexanal and linalool antimicrobial compositions as active component and in packaging micro Process fruit or vegetables
CN106543743A (en) * 2016-10-21 2017-03-29 北京化工大学 A kind of hydrolysate of the collagen containing linalool/edible antibacterial film of chitosan quaternary ammonium salt and preparation method thereof
CN107006593A (en) * 2017-03-10 2017-08-04 浙江省农业科学院 A kind of fruits and vegetables antibacterial shock absorbing foam

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110897005A (en) * 2019-11-15 2020-03-24 华南理工大学 Method for sterilizing gram-negative pathogenic bacteria in milk
CN110897005B (en) * 2019-11-15 2022-12-16 华南理工大学 Method for sterilizing gram-negative pathogenic bacteria in milk

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Application publication date: 20190906