CN110180610A - Reagent sequence loading method, structure and micro fluidic device - Google Patents
Reagent sequence loading method, structure and micro fluidic device Download PDFInfo
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- CN110180610A CN110180610A CN201910530656.7A CN201910530656A CN110180610A CN 110180610 A CN110180610 A CN 110180610A CN 201910530656 A CN201910530656 A CN 201910530656A CN 110180610 A CN110180610 A CN 110180610A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
Abstract
This application involves reagent sequence loading methods, structure and micro fluidic device, reagent sequence loading structure includes load communication port, target communication port, an at least target cavity and at least two load chambers of sequence setting, first load chamber load communication port and a target cavity corresponding by the first load pipeline connection, latter load chamber in its at the close-target rotation center by its connecting pipe in the target location of previous load pipeline be connected to previous load pipeline and latter load chamber in its far from target rotation center by its load pipeline be connected to corresponding to a target cavity.Reagent sequence loading structure, which is in constant centrifugal rotational speed, can realize the sequence load of reagent, realize simple and saved valve relevant cost；The time interval that different reagents loadeds are controlled by controlling centrifugal rotational speed has reserved the time to reagent reaction, has been particularly suitable for the situation that plurality of reagents loads respectively in order.
Description
Technical field
This application involves centrifugal microfluidic control fields, more particularly to reagent sequence loading method, reagent sequence loading structure
And micro fluidic device.
Background technique
Micro-fluidic (Microfluidics) refers to the handling liquids on submillimeter scale.It is by biological and chemical field institute
The basic operation unit being related to even collects the function in entire laboratory, including sampling, dilution, reaction, separation, detection etc.
On Cheng Yi small chip, therefore also known as chip lab (Lab-on-a-Chip).Centrifugal microfluidic control is under the jurisdiction of micro-fluidic one
A branch drives the flowing of liquid by centrifugal force, refer in particular to by rotation centrifugal microfluidic control device come using centrifugal force in Asia
The device of handling liquids on mm-scale.It by basic operation unit involved in biological and chemical field be integrated in one it is small-sized
On (disc-shaped) chip of dish-style.Other than micro-fluidic specific advantage, since centrifugal microfluidic control only needs an electricity
Machine provides power required for liquid manipulation, so whole equipment more concision and compact.Centrifugal microfluidic control is more and more answered
In care diagnostic.Microfluidic applications in vitro diagnostic field when, an important operation is exactly to allow reagent according to certain
Sequence reacts, and finally obtains diagnostic result.And to realize that plurality of reagents reacts according to certain sequence, it is exactly first
Realize that plurality of reagents is loaded into specified reaction chamber according to certain sequence.In traditional centrifugal microfluidic control, reagent it is suitable
Sequence load relies primarily on valve to realize, such as capillary valves, siphon valve, paraffin valve etc..Micro-fluidic chip according to setting revolving speed when
When sequence rotates, different reagents can successively break through different valves, to realize the sequence load of reagent.However, in centrifugal microfluidic
In control, the realization of these valves is all less susceptible to, and the use of valve often will increase the processing cost of entire micro-fluidic chip, Er Qieyong
Valve come realize sequence load often repeatability and reliability be difficult to be guaranteed.Machining accuracy of traditional capillary valves to pipeline
It is required that it is very high, and capillary valves itself can be related with the contact angle of liquid reagent on the surface of the material, and often different reagents needs
Different line sizes realizes the effects of capillary valves；Traditional siphon valve needs to do siphon piping hydrophily processing, this
The technique requirement of processing is very high, often substantially increases the processing cost of chip；The thawing of paraffin is often in traditional paraffin valve
It needs instrument to carry out corresponding temperature control, increases the design difficulty and cost of instrument.
Summary of the invention
Based on this, it is necessary to provide a kind of reagent sequence loading method, structure and micro fluidic device.
A kind of reagent sequence loading method comprising following steps: in micro fluidic device, each chamber that loads passes through respectively
It loads pipeline and is connected to corresponding target cavity, wherein the first load chamber is connected to external environment；Under apocarpy, add
The reagent carried in the first load chamber enters first object chamber by the first load pipeline；First loads the reagent in chamber
When liquid level is lower than the first object position in the first load pipeline, the second load chamber is added by first object position connection first
Chamber is carried to be connected to external environment, the reagent in load the second load chamber enters the second target cavity by the second load pipeline
Room, wherein the minimum range of the second load chamber and target rotation center is less than first object position and target rotation center
Distance；Sequence executes until the reagent completed in the last one load chamber enters the last one target cavity.
A kind of reagent sequence loading structure comprising load communication port, target communication port, at least a target cavity and sequence
At least two load chambers being arranged, each corresponding target cavity of the load chamber；The reagent sequence loading structure
With target rotation center；In each load chamber, communication port is loaded described in the first load chamber and is added by first
The corresponding target cavity of pipeline connection is carried, latter load chamber is connected at the target rotation center by it in it
Pipeline is connected to previous load pipeline and latter load chamber in it far from the target in the target location of previous load pipeline
The corresponding target cavity of pipeline connection is loaded by it at rotation center；Each target cavity is connected to the target connection
Mouthful.
A kind of micro fluidic device comprising the reagent sequence loading structure as described in any one.
Mentioned reagent sequence loading method and structure are applied in micro fluidic device, and reagent sequence is on the one hand only needed to add
The carrier such as micro fluidic device for carrying structure is in constant centrifugal rotational speed, so that it may which the sequence load for realizing reagent is not required to
Additional valve gear is wanted, realize simple and has saved valve relevant cost；On the other hand can also by control centrifugal rotational speed, thus
The time interval for controlling different reagents loadeds has reserved the time to reagent reaction, has been particularly suitable for plurality of reagents by suitable
The situation that sequence loads respectively；Another further aspect is conducive to simplify the processing technology and processing Quality Control cost of micro-fluidic chip.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of one embodiment of the application reagent sequence loading structure.Fig. 2 is the A of embodiment illustrated in fig. 1
Locate enlarged diagram.Fig. 3 is structure of the application using one embodiment of micro fluidic device of reagent sequence loading structure shown in Fig. 1
Schematic diagram.Fig. 4 is the direction the B-B schematic cross-sectional view of embodiment illustrated in fig. 3.Fig. 5 is to amplify signal at the C of embodiment illustrated in fig. 4
Figure.Fig. 6 is the schematic diagram of another direction of embodiment illustrated in fig. 3.Fig. 7 is the schematic diagram of another direction of embodiment illustrated in fig. 3.Fig. 8
For enlarged diagram at the D of embodiment illustrated in fig. 7.Fig. 9 is the schematic diagram of another direction of embodiment illustrated in fig. 7.Figure 10 is this Shen
It please be using the structural schematic diagram of another embodiment of micro fluidic device of reagent sequence loading structure shown in Fig. 1.Figure 11 is Figure 10 institute
Show enlarged diagram at the E of embodiment.Figure 12 is the structural schematic diagram of another embodiment of the application reagent sequence loading structure.Figure
13 be enlarged diagram at the F of embodiment illustrated in fig. 12.Figure 14 is enlarged diagram at the G of embodiment illustrated in fig. 12.Figure 15 is
The application uses the structural schematic diagram of one embodiment of micro fluidic device of reagent sequence loading structure shown in Figure 12.Figure 16 is Figure 15
Enlarged diagram at the H of illustrated embodiment.
Specific embodiment
Many details are explained in the following description in order to fully understand the application.But the application can be with
Much it is different from other way described herein to implement, those skilled in the art can be without prejudice to the application intension the case where
Under do similar improvement, therefore the application is not limited by the specific embodiments disclosed below.Unless otherwise defined, used herein
All technical and scientific terms it is identical as the normally understood meaning of the those skilled in the art for belonging to the application.At this
The term used in the description of application is only for the purpose of describing specific embodiments, it is not intended that in limiting this Shen
Please.Term " and or " used herein includes any and all combinations of one or more related listed items.
In the application one embodiment, a kind of reagent sequence loading method comprising following steps: in micro fluidic device
In, each chamber that loads loads the corresponding target cavity of pipeline connection by it respectively, wherein the first load chamber and external environment
Connection；Under apocarpy, the reagent in load the first load chamber enters first object chamber by the first load pipeline；The
When the liquid level of reagent in one load chamber is lower than first object position in the first load pipeline, the second load chamber passes through the
For the first load chamber of one target position connection to be connected to external environment, the reagent in load the second load chamber passes through the second load
Pipeline enters the second target cavity, wherein the minimum range of the second load chamber and target rotation center is less than first object position
It sets at a distance from target rotation center；Sequence executes until the reagent completed in the last one load chamber enters the last one mesh
Mark chamber.Mentioned reagent sequence loading method is applied in micro fluidic device, on the one hand only needs reagent sequence loading structure
Carrier such as micro fluidic device is in constant centrifugal rotational speed, so that it may which the sequence load for realizing reagent does not need additional
Valve gear is realized simple and has saved valve relevant cost；It on the other hand can also be by controlling centrifugal rotational speed, to control difference
The time interval of reagents loaded has reserved the time to reagent reaction, is particularly suitable for plurality of reagents and adds respectively in order
The situation of load；Another further aspect is conducive to simplify the processing technology and processing Quality Control cost of micro-fluidic chip.A reality wherein
It applies in example, a kind of reagent sequence loading method comprising the part steps or Overall Steps of following embodiment；That is, reagent sequence
Loading method includes some technical characteristics or all technical features below.Reagent sequence loads in one of the embodiments,
Method is realized using following any embodiment reagent sequence loading structure.
In micro fluidic device, each chamber that loads loads the corresponding target cavity of pipeline connection by it respectively, wherein the
One load chamber is connected to external environment；In one of the embodiments, in micro fluidic device, each chamber that loads passes through respectively
It loads pipeline and is connected to corresponding target cavity, comprising: in the micro-fluidic dress with any embodiment reagent sequence loading structure
In setting, each chamber that loads loads the corresponding target cavity of pipeline connection by it respectively.Wherein, it is respectively preset pair in load chamber
The reagent answered.Further, the position of chamber is loaded according to target centrifugal direction setting first in one of the embodiments,
To control centrifugal force for the active force of the reagent in the first load chamber.Under apocarpy, in load the first load chamber
Reagent by first load pipeline enter first object chamber；Scheduled centrifugal speed is used in one of the embodiments,
First object chamber is entered by the first load pipeline to control the reagent in load the first load chamber.
When the liquid level of reagent in first load chamber loads the first object position in pipeline lower than first, the second load
Chamber is connected to external environment by the first load chamber of first object position connection, and the reagent in load the second load chamber is logical
It crosses the second load pipeline and enters the second target cavity, wherein the minimum range of the second load chamber and target rotation center is less than
First object position is at a distance from target rotation center；Also that is, the liquid level of the reagent in current load chamber is lower than its load
When target position in pipeline, the reagent in latter load chamber is by first object position connection the first load chamber to be connected to
External environment enters target cavity corresponding to latter load chamber so as to load pipeline by latter load chamber.It is suitable
Sequence executes until the reagent completed in the last one load chamber enters the last one target cavity.This way it is possible to realize examination
The sequence of agent loads, and does not need additional valve gear, realizes simple and has saved valve relevant cost, can also be centrifuged by control
Revolving speed has been reserved the time to reagent reaction, has been particularly suitable for a variety of to control the time interval of different reagents loadeds
The situation that reagent loads respectively in order.Each target cavity merges setting in one of the embodiments,；Alternatively, passing through control
Centrifugal rotational speed, the length of each load pipeline and/or each load pipeline by area, to control the reagent in each load chamber
Loading speed.Each target cavity merges setting in one of the embodiments,；By control centrifugal rotational speed, respectively load pipeline
Length and/or each load pipeline by area, to control the loading speed of the reagent in each load chamber.Such design,
The loading speed for being conducive to control and adjust the reagent in each load chamber, to be suitable for differential responses environment.
In the application one embodiment, a kind of reagent sequence loading structure comprising load communication port, target connection
Mouth, at least a target cavity and at least two load chambers of sequence setting, the corresponding target cavity of each load chamber；Reagent is suitable
Sequence loading structure has target rotation center；In each load chamber, the first load chamber loads communication port and passes through first
The corresponding target cavity of pipeline connection is loaded, latter load chamber passes through its communicating pipe in it at close-target rotation center
Road is connected to previous load pipeline in the target location of previous load pipeline and latter load chamber is in it is rotated far from target
The corresponding target cavity of pipeline connection is loaded by it at the heart；Each target cavity is connected to target communication port.Mentioned reagent is suitable
Sequence loading structure is applied in micro fluidic device, on the one hand only needs for example micro-fluidic dress of the carrier of reagent sequence loading structure
Set and be in constant centrifugal rotational speed, so that it may the sequence load for realizing reagent does not need additional valve gear, realize it is simple and
Valve relevant cost is saved；It on the other hand can also be by controlling centrifugal rotational speed, thus between the time for controlling different reagents loadeds
Every, to reagent reaction reserved the time, be particularly suitable for the situation that plurality of reagents loads respectively in order；Another further aspect
Be conducive to simplify the processing technology and processing Quality Control cost of micro-fluidic chip.A kind of reagent is suitable in one of the embodiments,
Sequence loading structure comprising the part-structure or entire infrastructure of following embodiment；That is, reagent sequence loading structure includes below
Some technical characteristics or all technical features.A kind of reagent sequence loading structure in one of the embodiments, comprising load
Communication port, target communication port, at least a target cavity and at least two load chambers of sequence setting, each load chamber corresponding one
Target cavity.A target cavity can correspond to multiple load chambers in one of the embodiments, or each target cavity merges
Setting, i.e., be only arranged a target cavity；Load chamber is identical as the quantity of target cavity in one of the embodiments, and each
Load chamber is arranged in a one-to-one correspondence with each target cavity.Reagent sequence loading structure only includes one in one of the embodiments,
Target cavity.The reagent of such design, all load chambers is all sequentially loaded into target cavity.
Reagent sequence loading structure has target rotation center；Target rotation center can be entity, be also possible to diastema；
Target rotation center can be inside reagent sequence loading structure, can also be empty outside reagent sequence loading structure, but usually
Outside reagent sequence loading structure, that is, target rotation center is the opposite object of reference an of outside.A reality wherein
Apply in example, reagent sequence loading structure for being set in micro fluidic device, and target rotation center be micro fluidic device from
The heart in the heart.Further, reagent sequence loading structure is rotated in each load chamber far from target in one of the embodiments,
Load pipeline is respectively equipped at center, load chamber passes through a target cavity corresponding to load pipeline connection load chamber；And
In addition to the first LOADED CAVITY room, reagent sequence loading structure is respectively equipped with connection in remaining load chamber at target rotation center
Pipeline, load chamber are connected to the load pipeline of previous load chamber by connecting pipe.
At least one load pipeline bending setting in one of the embodiments,；Basis respectively adds in one of the embodiments,
The length of each load pipeline is arranged for the target loading speed for carrying chamber and/or each load pipeline passes through area.One wherein
In embodiment, at least one load pipeline bending setting；According to the target loading speed of each load chamber, each load pipeline is set
Length and/or respectively load pipeline pass through area.Such design, can be convenient according to reaction requirement or application demand,
The time interval for controlling or adjusting different reagents loadeds has reserved the time to reagent reaction, has been particularly suitable for a variety of examinations
The situation that agent loads respectively in order.
In each load chamber, first loads chamber load communication port and by corresponding to the first load pipeline connection
One target cavity, latter load chamber pass through its connecting pipe in the mesh of previous load pipeline in it at close-target rotation center
Previous load pipeline and latter load chamber are connected at cursor position in it far from passing through its load pipeline company at target rotation center
Lead to a corresponding target cavity；Load communication port can be used as liquid injection port in one of the embodiments, and reagent is injected into
In first load chamber, each load chamber is respectively equipped with liquid injection port in one of the embodiments, and after injecting reagent,
Other liquid injection port encapsulation process other than the liquid injection port of the first load chamber, so that just can only other than target communication port
External environment is connected to by the liquid injection port that load communication port i.e. first loads chamber.Alternatively, each in one of the embodiments,
It loads chamber and is equipped with loading opening and its load capping, when needing that reagent is added, open load capping and expose loading opening, it will
Reagent is added in load chamber, covers load capping then to seal loading opening.Further, one embodiment wherein
In, the second load chamber is connected to first by the second connecting pipe at close-target rotation center in it at first object position
Load pipeline and the second load chamber in its far from target rotation center by a mesh that the second load pipeline connection is corresponding
Chamber is marked, first object position is located in the first load pipeline, and the minimum range of the second load chamber and target rotation center is small
In first object position at a distance from target rotation center, and the maximum distance of the first load chamber and target rotation center is less than
First object position is at a distance from target rotation center；Remaining each load chamber is right with the previous load chamber of this sequential communication and institute
The target cavity answered.Each load chamber of cooperation sequence setting in this way realizes the effect of sequence load control, so that in addition to
First load chamber except other load chambers, reagent therein all it is previous load chamber reagents loaded to a certain extent
Or even it is just entered in corresponding target cavity after the completion of load.
Each target cavity is connected to target communication port.That is, other load chambers other than the first load chamber, not only lead to
Its connecting pipe and the first load chamber load communication port are crossed, mesh is also connected to by load pipeline and corresponding target cavity
Mark communication port.Further, each target cavity is respectively communicated with target communication port in one of the embodiments,.One wherein
In embodiment, each target cavity is connected to a target communication port by same target connecting pipe.Mesh in one of the embodiments,
Mark communication port quantity be it is multiple, each target cavity is respectively communicated with a target communication port.Such design is formed a phase
For target rotation center by entering mesh from each load chamber under the influence of centrifugal force as far as close reagent sequence load system
Mark chamber.
Load communication port is set to the first load chamber closest to the position of target rotation center in one of the embodiments,
It sets place or the first load chamber passes through the first ventilation pipe connection load communication port and load communication port and target rotation center
Maximum distance be more than or equal to first load the first ventilation pipe of chamber position at a distance from target rotation center；That is,
Load communication port may be disposed in the first load chamber, also may be disposed at outside the first LOADED CAVITY room.In one of the embodiments,
A target communication port is respectively set in each target cavity in reagent sequence loading structure, alternatively, each target cavity passes through target respectively
Connecting pipe is connected to target communication port and target communication port and the maximum distance of target rotation center are more than or equal to each target cavity
The position of target connecting pipe is connected at a distance from target rotation center.That is, target communication port may be disposed in target cavity, also
It may be disposed at outside target cavity.Load communication port is set to the first load chamber closest to target in one of the embodiments,
At the position of rotation center or first load chamber by the first ventilation pipe connection load communication port and load communication port with
The maximum distance of target rotation center is more than or equal in the position and target rotation of first load the first ventilation pipe of chamber
The distance of the heart；A target communication port is respectively set in each target cavity in reagent sequence loading structure, alternatively, each target cavity is distinguished
Target communication port is connected to by target connecting pipe and target communication port and the maximum distance of target rotation center are more than or equal to respectively
Target cavity is connected to the position of target connecting pipe at a distance from target rotation center.
Chamber is respectively loaded in one of the embodiments, and pipeline is loaded in a corresponding target cavity close to mesh by it
Mark the target cavity that connection is corresponding at rotation center；Also that is, LOADED CAVITY room connection target cavity is closer to target rotation
The position at center is conducive in this way in the relatively small position output reagent of centrifugal force into target cavity, and in centrifugation masterpiece
Position of the target cavity far from target rotation center is come together under.Chamber Yu Qiyuan is respectively loaded in one of the embodiments,
From all having collapsed shape at target rotation center；In one of the embodiments, respectively load chamber in up-side down triangle, circle,
Shuttle shape or ellipse etc..Such design advantageously allows reagent and is exported from collapsed shape so as to now load complete effect.
Each target cavity is in it far from all having collapsed shape at target rotation center in one of the embodiments,；One wherein
In embodiment, each target cavity is in up-side down triangle, circle, shuttle shape or ellipse etc..On the one hand such design is conducive to clear up
Or the reagent in output target cavity, being on the other hand conducive to cooperation has the embodiment of collection chamber and waste chamber to realize
The effect of reagent output.The center of each load chamber phase at a distance from target rotation center in one of the embodiments,
With or it is close, or each load chamber closest to the position of target rotation center the identical or phase at a distance from target rotation center
Closely；Wherein, close 111% and minimum value for maximum value no more than average value is not less than the 90% of average value；One wherein
In embodiment, close 108% and minimum value for maximum value no more than average value is not less than the 91% of average value.It is such to set
Meter is conducive to control the centrifugal force situation being subject in the reagent of load chamber, to assist cooperation centrifugal speed control reagent
Load.In one embodiment, connecting pipe includes increase in pipeline, transition conduit, decline pipeline and the connection of sequence setting
Pipeline, connecting pipe are connected to previous load pipeline in the target location of previous load pipeline；In transition conduit and target rotation
The maximum distance of the heart is less than the maximum distance of increase in pipeline and target rotation center, declines the maximum of pipeline and target rotation center
The minimum range of distance and connecting pipe and target rotation center.Such design is conducive to that target position is cooperated to form gas
The control effect of pressure valve, so that reagent of the reagent only in previous load chamber in load chamber enters corresponding target cavity
After room, can just it enter in the target cavity corresponding to it.Further, target position is formed with bent area.It is such to set
Meter is conducive to the length and resistance that increase corresponding load pipeline, plays the effect for increasing liquid flowing resistance.One wherein
In embodiment, each load chamber loads pipeline by it and is connected to institute at close-target rotation center in a corresponding target cavity
A corresponding target cavity；And/or each load chamber in it far from all having collapsed shape at target rotation center；And/or
Each target cavity is in it far from all having collapsed shape at target rotation center；And/or center and the mesh of each load chamber
The distance for marking rotation center is same or similar, or each load chamber is closest in the position of target rotation center and target rotation
The distance of the heart is same or similar；Wherein, close 111% and minimum value for maximum value no more than average value is not less than average value
90%；And/or connecting pipe includes increase in pipeline, transition conduit, decline pipeline and the connecting pipe of sequence setting, connecting tube
Road is connected to previous load pipeline in the target location of previous load pipeline；The maximum distance of transition conduit and target rotation center
Less than the maximum distance of increase in pipeline and target rotation center, the maximum distance and connection of decline pipeline and target rotation center
The minimum range of pipeline and target rotation center.
Reagent sequence loading structure further includes collection chamber and waste chamber, collection chamber in one of the embodiments,
It is greater than the maximum distance of target cavity and target rotation center with the minimum range of target rotation center, waste chamber and target are revolved
The minimum range for turning center is greater than the maximum distance of target cavity and target rotation center；The bottom of target cavity is equipped with filtering
Area, bottom are the position far from target rotation center；Collection chamber is connected to filtering area by collection conduit, and waste chamber passes through useless
Liquid pipe road is connected to filtering area；Target communication port includes collecting communication port and waste liquid communication port；Center and mesh relative to filtering area
The line of rotation center is marked, collection chamber and waste chamber are located at the two sides of line, collect communication port and waste liquid communication port
Also the two sides of line are located at；It collects communication port and is set to collection chamber closest at the position of target rotation center, or
Collecting communication port, ventilation pipe is connected to collection chamber and collection communication port and the maximum distance of target rotation center are big by collecting
The position of ventilation pipe is collected at a distance from target rotation center in being equal to collection chamber's connection；Waste liquid communication port is set to waste liquid
Chamber is closest at the position of target rotation center or waste liquid communication port by waste liquid ventilation pipe is connected to waste chamber and useless
Liquid communication port is more than or equal to position and the target that waste chamber is connected to waste liquid ventilation pipe with the maximum distance of target rotation center
The distance of rotation center；Target cavity sequence passes through collection conduit and communication port is collected in collection chamber's connection, and sequentially by useless
Liquid pipe road and waste chamber are connected to waste liquid communication port.Further, filter membrane is equipped with inside filtering area in one of the embodiments,
Such as pellosil etc..
Reagent sequence loading structure only includes a target cavity, reagent sequence loading structure in one of the embodiments,
Including four load chambers；In each load chamber, the first load chamber loads communication port and by the first load pipeline connection
Target cavity；Second load chamber is connected at first object position at close-target rotation center by the second connecting pipe in it
Logical first load pipeline and the second load chamber are in it far from being connected to target cavity by the second load pipeline at target rotation center
Room, wherein first object position is located in the first load pipeline, and the minimum range of the second load chamber and target rotation center is small
In first object position at a distance from target rotation center, and the maximum distance of the first load chamber and target rotation center is less than
First object position is at a distance from target rotation center；Third loads chamber and is connected at close-target rotation center by third in it
It threads a pipe and loads pipeline and third load chamber in it far from logical at target rotation center in the second target location connection second
Cross third load pipeline connection target cavity, wherein the second target position be located at second load pipeline in, third load chamber with
The minimum range of target rotation center less than the second target position at a distance from target rotation center, and the second load chamber and mesh
The maximum distance of mark rotation center is less than the second target position at a distance from target rotation center；4th load chamber is close in its
Third is connected in third target location by the 4th connecting pipe at target rotation center and loads pipeline and the 4th load chamber
Target cavity is connected to far from the 4th load pipeline is passed through at target rotation center in it, wherein third target position is located at third
It loads in pipeline, the minimum range of the 4th load chamber and target rotation center is less than third target position and target rotation center
Distance, and the maximum distance of third load chamber and target rotation center is less than third target position and target rotation center
Distance.Such design by taking four load chambers and a target cavity as an example, but is equally applicable to the LOADED CAVITY of other quantity
The reagent sequence loading structure of room and/or multiple target cavities.
A kind of micro fluidic device in one of the embodiments, comprising such as any embodiment reagent sequence loading structure.
Micro fluidic device is micro-fluidic chip, i.e. centrifugal microfluidic control chip in one of the embodiments,.One embodiment wherein
In, micro fluidic device includes at least two reagent sequence loading structures.
A kind of reagent sequence loading structure is as shown in Figure 1 in one of the embodiments, comprising load communication port 219,
Four load chambers of target communication port 259, target cavity 250 and sequence setting, four load chambers include the first LOADED CAVITY
Room 210, second loads chamber 220, third load chamber 230 and the 4th load chamber 240, and each chamber that loads corresponds to same target
Chamber 250, referring to Figure 2 together, Fig. 3, Fig. 4 and Fig. 5, reagent sequence loading structure 200 be set in micro fluidic device 100 or
Reagent sequence loading structure 200 is set on micro fluidic device 100, and reagent sequence loading structure 200 has in target rotation
The heart 300；Target rotation center 300 is the centrifugation center of micro fluidic device 100.Reagent sequence loading structure is in each LOADED CAVITY
Room far from being correspondingly provided with load pipeline respectively at target rotation center 300, including the first load pipeline 212, second loads pipeline
222, third load pipeline 232 and the 4th load pipeline 242；In four load chambers, the first load chamber 210 is logical by first
The connection of feed channel 211 loads communication port 219 and is connected to target cavity 250 by the first load pipeline 212；Second load chamber
220 are connected to first by the second connecting pipe 221 at close-target rotation center 300 in it at first object position 214 adds
Carry pipeline 212 and second load chamber 220 in its far from target rotation center 300 by second load pipeline 222 be connected to mesh
Mark chamber 250, wherein first object position 214 is located in the first load pipeline 212, and the second load chamber 220 and target rotate
The minimum range at center 300 is less than first object position 214 at a distance from target rotation center 300, and the first load chamber 210
It is less than at a distance from first object position 214 and target rotation center 300 with the maximum distance of target rotation center 300；Third adds
Chamber 230 is carried to be connected at the second target position 224 at close-target rotation center 300 by third connecting pipeline 231 in it
Second load pipeline 222 and third load chamber 230 in its far from target rotation center 300 pass through third load pipeline 232
It is connected to target cavity 250, wherein the second target position 224 is located in the second load pipeline 222, and third loads chamber 230 and mesh
Mark rotation center 300 minimum range less than the second target position 224 with target rotation center 300 at a distance from, and second load
The maximum distance of chamber 220 and target rotation center 300 less than the second target position 224 at a distance from target rotation center 300；
4th load chamber 240 passes through the 4th connecting pipe 241 in third target position 234 in it at close-target rotation center 300
Place is connected to third load pipeline 232 and the 4th load chamber 240 is managed in it far from the 4th load is passed through at target rotation center 300
Road 242 is connected to target cavity 250, wherein third target position 234 is located in third load pipeline 232, the 4th load chamber
240 are less than at a distance from third target position 234 and target rotation center 300 with the minimum range of target rotation center 300, and the
The maximum distance of three load chambers 230 and target rotation center 300 is less than third target position 234 and target rotation center 300
Distance.Wherein, the first load pipeline 212, third load pipeline 232 and the 4th load bending setting of pipeline 242.Target cavity
250 are connected to target communication port by target connecting pipe 251 at its target connection position 252 by close-target rotation center 300
259.Connecting pipe includes increase in pipeline, transition conduit, decline pipeline and the connecting pipe of sequence setting, with the 4th connecting pipe
For 241, as shown in Figures 1 and 2 comprising the 4th increase in pipeline 2411, the 4th transition conduit the 2412, the 4th decline pipeline
2413 and the 4th connecting pipe 2414, the 4th connecting pipe 2414 third load pipeline is connected at third target position 234
232, the 4th load chamber 240 of the 4th increase in pipeline 2411 connection, the 4th transition conduit 2412 and target rotation center 300 are most
Maximum distance, fourth decline pipeline 2413 and target of the big distance less than the 4th increase in pipeline 2411 and target rotation center 300
The minimum range of the maximum distance of rotation center 300 and the 4th connecting pipe 2414 and target rotation center 300.Reagent sequence
Loading structure 200 can also further regard to Fig. 6 and Fig. 7 in the position of micro fluidic device 100, it is to be understood that at one
One, two or more reagent sequence loading structures 200 can be equipped in micro fluidic device 100.Referring to Fig. 8, the first load
Pipeline 212 is connected to target cavity 250 in the first connection position 213, and the second load pipeline 222 is connected to target in the second connection position 223
Chamber 250, third load pipeline 232 in the 4th load pipeline 242 of the connection of third connecting position 233, and the 4th load pipeline 242 is in the
Four connection positions 243 are connected to target cavity 250.Load the position of communication port 219 and target communication port 259 in micro fluidic device 100
It sets as shown in Figure 9.In one of the embodiments, as shown in Figure 10, reagent sequence loading structure 200 is located at micro fluidic device
100 non-center position, also referring to Figure 11, the first load chamber 210, second loads chamber 220, third loads chamber
230, the 4th load chamber 240 and target cavity 250 are rounded.
The example of a concrete application is given below, chamber is loaded toward first as liquid injection hole by load communication port 219
Liquid reagent is injected in 210.Liquid is preset in second load chamber 220, third load chamber 230 and the 4th load chamber 240
There are liquid injection hole, fluid injection is completed for body reagent or the second load chamber 220, third load chamber 230 and the 4th load chamber 240
Liquid injection hole is sealed, the mode of sealing of hole includes but is not limited to paraffin envelope, mucilage sealing, adhesive tape envelope etc..In an application example, second
Liquid storage container is preset in load chamber 220, third load chamber 230 and the 4th load chamber 240, reagent is preset at liquid storage
In container.Reagent is set in hot melt wrapping layer in one of the embodiments, and hot melt wrapping layer is set in load chamber.
Reagent is set in wrapping layer in one of the embodiments, and wrapping layer is set in load chamber and wrapping layer is equipped with and uses
The closed opening of hot melt layer.Liquid storage container is viscous in one of the embodiments, is set in load chamber.One embodiment wherein
In, liquid storage container has aluminium foil layer.Liquid storage container is equipped with opening, thorn part, elastic component and sealer in one of the embodiments,
Sealer is fixed in load chamber for closing opening, elastic component one end connection thorn part, the other end, and thorn part is used to match in centrifugation
It closes elastic component and generates displacement to puncture sealer to release reagent.First cooperation load chamber liquid storage mode, using heating or
The centrifugation of person's fair speed loads liquid, that is, reagent in chamber 210 for first and is discharged into target cavity 250.Most start, due to
Junction, that is, first object position 214 of second connecting pipe 221 and the first load chamber 210 is not communicated with the atmosphere directly,
Therefore the liquid of the second load chamber 220, third load chamber 230 and the storage in the 4th load chamber 240 can not be discharged into
In target cavity 250.Then, high speed is centrifuged, when the liquid in the first load chamber 210 is flowed by the first load pipeline 212
Enter to target cavity 250, until the liquid levels in the first load pipeline 212 are connected to lower than the first load pipeline 212 with second
When the junction of pipeline 221, the second load chamber 220 is connected to ambient atmosphere.High speed centrifugation, second loads in chamber 220
Liquid by second load pipeline 222 be flowed into target cavity 250, until second load pipeline 222 in liquid levels
When lower than i.e. the second target position 224 in junction of the second load pipeline 222 and third connecting pipeline 231, third loads chamber
230 are communicated with the atmosphere.High speed centrifugation, third load the liquid in chamber 230 and are flowed into target by third load pipeline 232
In chamber 250, until the liquid levels in third load pipeline 232 are lower than third load pipeline 232 and the 4th connecting pipe 241
Junction, that is, third target position 234 when, the 4th load chamber 240 is communicated with the atmosphere.High speed centrifugation, the 4th load chamber
Liquid in 240 is flowed into target cavity 250 by the 4th load pipeline 242.Added by the first load chamber 210, second
It carries chamber 220, the sequence that the third load load chamber 240 of chamber the 230, the 4th is connected to ambient atmosphere, respectively adds to realize
It carries the indoor liquid reagent of chamber to be successively discharged into target cavity 250, to realize the sequence load of reagent.It is each in order to control
The interval of chamber liquid release time is loaded, each pipeline that loads can be designed as continuous curved snake pipe, it should be pointed out that
It is that the design of snake pipe can play the effect for increasing liquid flowing resistance, each load pipeline can also be sized to
Small size.It, can also be by controlling centrifugal rotational speed, to control the liquid flowing in each load chamber or in centrifugal process
Speed, and then control the time interval of the release of each LOADED CAVITY indoor liquid.Specific centrifugal speed is set according to the actual situation
Meter or adjustment, are not considered as the additional limitation to each embodiment of the application.It should be pointed out that reagent sequence loads
The sequence that plurality of reagents may be implemented in device loads, and including but not limited to four kinds.
A kind of reagent sequence loading structure is as shown in figure 12 in one of the embodiments, and reagent sequence loading structure is also
Including collection chamber 260 and waste chamber 270, the minimum range of collection chamber 260 and target rotation center 300 is greater than target cavity
The minimum range of the maximum distance of room 250 and target rotation center 300, waste chamber 270 and target rotation center 300 is greater than mesh
Mark the maximum distance of chamber 250 and target rotation center 300；First load pipeline 212, second loads pipeline 222, third load
Pipeline the 232, the 4th loads pipeline 242 and bends setting, by taking the 4th load pipeline 242 as an example comprising the first of sequence setting
It bends pipeline 2421, second and bends pipeline 2422, the third bending bending bending pipeline of pipeline the 2424, the 5th of pipeline the 2423, the 4th
2425, wherein the 5th bending pipeline 2425 is connected to target cavity 250；Also referring to Figure 13, third loads the third of pipeline 232
Target position 234 is located between the 6th bending pipeline 2321 that third load pipeline 232 is connected and the 7th bending pipeline 2322,
4th connecting pipe 2414 is connected to third load pipeline 232 at third target position 234, and third target position 234 is formed with
Bent area.Please continue to refer to Figure 12, target cavity 250 is in triangle is inverted, also referring to Figure 14, the receipts of target cavity 250
I.e. bottom is equipped with filtering area 253 at contracting shape, and collection chamber 260 is connected to filtering area 253, waste chamber by collection conduit 261
270 are connected to filtering area 253 by waste solution channel 271；Target communication port 259 includes collecting communication port 269 and waste liquid communication port
279；Relative to the center of filtering area 253 and the line of target rotation center 300, collection chamber 260 and waste chamber 270 are distinguished
Positioned at the two sides of line, collects communication port 269 and waste liquid communication port 279 is also located at the two sides of the line；Collect communication port
269 are set to collection chamber 260 closest at the position of target rotation center 300, or to collect communication port 269 logical by collecting
Feed channel is connected to collection chamber 260 and collects the maximum distance of communication port 269 and target rotation center 300 more than or equal to collecting chamber
The connection of room 260 collects the position of ventilation pipe at a distance from target rotation center 300；Waste liquid communication port 279 is set to waste liquid chamber
Room 270 is closest at the position of target rotation center 300 or waste liquid communication port 279 by waste liquid ventilation pipe is connected to waste liquid
Chamber 270 and waste liquid communication port 279 are more than or equal to waste chamber 270 with the maximum distance of target rotation center 300 and are connected to waste liquid
The position of ventilation pipe is at a distance from target rotation center 300；250 sequence of target cavity passes through collection conduit 261 and collecting chamber
Communication port 269 is collected in the connection of room 260, and sequence is connected to waste liquid communication port 279 by waste solution channel 271 and waste chamber 270.
Micro fluidic device 100 as shown in figure 15, also referring to Figure 16, manage by the first load in one of the embodiments,
Road 212 is connected to target cavity 250 in the first connection position 213, and the second load pipeline 222 is connected to target cavity in the second connection position 223
Room 250, third load pipeline 232 and are connected to target cavity 250 in third connecting position 233, and the 4th load pipeline 242 is in the 4th connection
Position 243 is connected to target cavity 250.First connection position 213, second is connected to position 223, third connecting position 233 and the 4th connection position 243
It is respectively positioned on the position that target cavity 250 leans on close-target rotation center 300.First load chamber 210, second loads chamber 220, the
Three load chambers 230 and the 4th load chamber 240 are rounded, and target cavity 250 is in inversion triangle.
Below by taking the process of nucleic acid purification as an example, then provide a specific example.In molecular diagnostic procedure, nucleic acid is mentioned
Take the nucleic acid purification in step very crucial, and since nucleic acid purification is related to the sequence load of several liquid reagents, often
It is difficult to be integrated into micro-fluidic chip.Firstly, the sample after cracking is injected into the first load chamber by load communication port 219
In 210, cleaning solution 1, cleaning solution 2, eluent pass through liquid injection hole respectively or loading opening be added to the second load chamber 220,
Third loads chamber the 230, the 4th and loads in chamber 240, then seals liquid injection hole or loading opening.It can also be pre- using reagent
Cleaning solution 1, cleaning solution 2, eluent are preset in three liquid storage containers by the method set respectively, and three liquid storage containers are distinguished again
It is preset at the second load chamber 220, third load chamber the 230, the 4th loads in chamber 240.Then centrifugal microfluidic control chip
The center of circle is fixed on motor rotation axis, and 1500rpm rotates clockwise centrifugal microfluidic control chip, and the first load chamber 210 is by adding
Communication port 219 is carried to be communicated with the atmosphere.Sample outflow in first load chamber 210 after cracking, begins through snakelike first and adds
When carrying the pellosil that pipeline 212 passes through in filtering area 253, nucleic acid such as DNA or RNA after cracking in sample are adsorbed on pellosil
On, waste liquid is fully entered under the action of Coriolis in waste chamber 270.Liquid levels in the first load pipeline 212
When lower than the junction of the second connecting pipe 221 and the first load pipeline 212, the second load chamber 220 passes through the first LOADED CAVITY
Room 210 is connected with atmosphere, and the cleaning solution 1 in the second load chamber 220 flows out, right when by pellosil in filtering area 253
Nucleic acid on Silicon moulds is cleaned, and waste liquid is fully entered under the action of Coriolis in waste chamber 270.When the second load
When liquid levels in pipeline 222 load the junction of pipeline 222 and third connecting pipeline 231 lower than second, third LOADED CAVITY
Room 230 is connected by the first load chamber 210 and the second load chamber 220 with atmosphere, and third loads the cleaning in chamber 230
Liquid 2 flows out, and when by pellosil in filtering area 253, cleans to the nucleic acid on Silicon moulds, work of the waste liquid in Coriolis
It is fully entered under in waste chamber 270.1500rpm rotates counterclockwise centrifugal microfluidic control chip, when in third load pipeline 232
Liquid levels lower than third load pipeline 232 and the 4th connecting pipe 241 connector when, the 4th load chamber 240 passes through
First load chamber 210, second loads chamber 220, third load chamber 230 is connected with atmosphere, in the 4th load chamber 240
Eluent outflow the nucleic acid on pellosil is eluted, the nucleic acid after elution when by pellosil in filtering area 253
Solution is fully entered under the action of Coriolis force in the collection chamber 260 of nucleic acid.It should be pointed out that each load pipeline is set
It is calculated as continuous curved snake pipe, the effect for increasing liquid flowing resistance can be played, to control in each load chamber
Reagent reaches slow release, and then controls the time interval of the release of each first LOADED CAVITY indoor liquid, reaches each reagent
The purpose sufficiently reacted with nucleic acid.Such design, can by control centrifugal rotational speed, thus control different reagents loadeds when
Between be spaced, to reagent reaction reserved the time.Reagent sequence loading device applied to centrifugal microfluidic control only needs simply
Change can realize any number of sequence load.
It should be noted that the other embodiments of the application further include, the mutually group of the technical characteristic in the various embodiments described above
Close reagent sequence loading method, structure and the micro fluidic device that is formed by, can implement.
Each technical characteristic of above embodiments can be combined arbitrarily, for simplicity of description, not to above-described embodiment
In each technical characteristic it is all possible combination be all described, as long as however, the combination of these technical characteristics be not present lance
Shield all should be considered as described in this specification.Above embodiments only express the several embodiments of the application, retouch
State more specific and detailed, but it cannot be understood as the limitations to claim.It should be pointed out that for this
For the those of ordinary skill in field, without departing from the concept of this application, various modifications and improvements can be made, this
The protection scope of the application is belonged to a bit.Therefore, the scope of patent protection of the application should be determined by the appended claims.
Claims (10)
1. a kind of reagent sequence loading method, which comprises the following steps:
In micro fluidic device, each chamber that loads loads the corresponding target cavity of pipeline connection by it respectively, wherein first adds
Chamber is carried to be connected to external environment；
Under apocarpy, the reagent in load the first load chamber enters first object chamber by the first load pipeline；
When the liquid level of reagent in first load chamber loads the first object position in pipeline lower than first, the second load chamber
By the first load chamber of first object position connection to be connected to external environment, reagent in load the second load chamber passes through the
Two load pipelines enter the second target cavity, wherein the minimum range of the second load chamber and target rotation center is less than first
Target position is at a distance from target rotation center；
Sequence executes until the reagent completed in the last one load chamber enters the last one target cavity.
2. reagent sequence loading method according to claim 1, which is characterized in that each target cavity merges setting；With/
Or,
By control centrifugal rotational speed, the length of each load pipeline and/or each load pipeline by area, to control each LOADED CAVITY
The loading speed of reagent in room.
3. a kind of reagent sequence loading structure, which is characterized in that including loading communication port, target communication port, at least a target cavity
At least two load chambers of room and sequence setting, each corresponding target cavity of the load chamber；
The reagent sequence loading structure has target rotation center；
In each load chamber, load communication port (i.e. liquid injection port, it is contemplated that other add described in the first load chamber
Carrying chamber also can be set liquid injection port, so having changed title to it) and pass through a target corresponding to the first load pipeline connection
Chamber, latter load chamber pass through its connecting pipe in the target of previous load pipeline in it at the target rotation center
Previous load pipeline and latter load chamber are connected at position in it far from passing through its load pipeline at the target rotation center
The corresponding target cavity of connection；
Each target cavity is connected to the target communication port.
4. reagent sequence loading structure according to claim 3, which is characterized in that the load communication port is set to described
One load chamber is closest at the position of the target rotation center or the first load chamber passes through the first ventilation pipe
It is connected to the load communication port and the load communication port and the maximum distance of the target rotation center is more than or equal to described the
The position of first ventilation pipe described in one load chamber is at a distance from the target rotation center；And/or the reagent is suitable
A target communication port is respectively set in each target cavity in sequence loading structure, alternatively, each target cavity leads to respectively
Cross the maximum distance that target connecting pipe is connected to the target communication port and the target communication port and the target rotation center
More than or equal to the position that each target cavity is connected to the target connecting pipe at a distance from the target rotation center.
5. reagent sequence loading structure according to claim 3, which is characterized in that at least one load pipeline bending is set
It sets；And/or the length of each load pipeline and/or each described is arranged according to the target loading speed of each load chamber
Load pipeline passes through area.
6. reagent sequence loading structure according to claim 3, which is characterized in that each load chamber is managed by its load
Road is connected to a corresponding target cavity in a corresponding target cavity at the target rotation center；
And/or each load chamber in it far from all having collapsed shape at the target rotation center；
And/or each target cavity in it far from all having collapsed shape at the target rotation center；
And/or the center of each load chamber same or similar or each institute at a distance from the target rotation center
The position for stating load chamber closest to the target rotation center is same or similar at a distance from the target rotation center；Its
In, close 111% and the minimum value for maximum value no more than average value is not less than the 90% of average value；
And/or the connecting pipe includes increase in pipeline, transition conduit, decline pipeline and the connecting pipe of sequence setting, it is described
Connecting pipe is connected to previous load pipeline in the target location of previous load pipeline；The transition conduit and the target rotate
The maximum distance at center be less than the maximum distance of the increase in pipeline and the target rotation center, the decline pipeline with it is described
The minimum range of the maximum distance of target rotation center and the connecting pipe and the target rotation center.
7. the reagent sequence loading structure according to any one of claim 3 to 6, which is characterized in that only include a mesh
Mark chamber.
8. reagent sequence loading structure according to claim 7, which is characterized in that it further include collection chamber and waste chamber,
The minimum range of the collection chamber and the target rotation center is greater than the target cavity and the target rotation center
The minimum range of maximum distance, the waste chamber and the target rotation center is greater than the target cavity and the target is revolved
Turn the maximum distance at center；
The bottom of the target cavity is equipped with filtering area, and the collection chamber is connected to the filtering area by collection conduit, described
Waste chamber is connected to the filtering area by waste solution channel；
The target communication port includes collecting communication port and waste liquid communication port；Center and the target relative to the filtering area
The line of rotation center, the collection chamber and the waste chamber are located at the two sides of the line, the collection connection
Mouth is also located at the two sides of the line with the waste liquid communication port；
The collection communication port is set to the collection chamber closest at the position of the target rotation center or the receipts
Collection communication port is connected to the collection chamber and communication port and the target rotation center collected by collecting ventilation pipe
Maximum distance be more than or equal to the collection chamber be connected to it is described collect ventilation pipe position and the target rotation center away from
From；
The waste liquid communication port is set to the waste chamber closest at the position of the target rotation center or described useless
Liquid communication port is connected to the waste chamber and the waste liquid communication port and the target rotation center by waste liquid ventilation pipe
Maximum distance be more than or equal to the waste chamber be connected to the waste liquid ventilation pipe position and the target rotation center away from
From；
The target cavity sequence is connected to the collection communication port by the collection conduit and the collection chamber, and sequence is logical
It crosses the waste solution channel and the waste chamber is connected to the waste liquid communication port.
9. reagent sequence loading structure according to claim 7, which is characterized in that including the four load chambers；
In each load chamber, communication port is loaded described in the first load chamber and by described in the first load pipeline connection
Target cavity；
Second load chamber is connected at first object position at the target rotation center by the second connecting pipe in it
Lead to the first load pipeline and the second load chamber connects in it far from the second load pipeline is passed through at the target rotation center
Lead to the target cavity, wherein the first object position be located at it is described first load pipeline in, it is described second load chamber with
The minimum range of the target rotation center is less than the first object position at a distance from the target rotation center, and described
The maximum distance of first load chamber and the target rotation center is less than in the first object position and target rotation
The distance of the heart；
Third loads chamber and is connected by third connecting pipeline in the second target location at the target rotation center in it
Lead to it is described second load pipeline and third load chamber in its far from the target rotation center by third load pipeline company
Lead to the target cavity, wherein second target position is located in the second load pipeline, third load chamber with
The minimum range of the target rotation center is less than second target position at a distance from the target rotation center, and described
The maximum distance of second load chamber and the target rotation center is less than in second target position and target rotation
The distance of the heart；
4th load chamber is connected by the 4th connecting pipe in third target location at the target rotation center in it
Lead to the third load pipeline and the 4th load chamber connects in it far from the 4th load pipeline is passed through at the target rotation center
Lead to the target cavity, wherein the third target position be located at the third load pipeline in, it is described 4th load chamber with
The minimum range of the target rotation center is less than the third target position at a distance from the target rotation center, and described
Third loads chamber and the maximum distance of the target rotation center is less than in the third target position and target rotation
The distance of the heart.
10. a kind of micro fluidic device, which is characterized in that including the reagent sequence load knot as described in any one of claim 3 to 9
Structure.
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