CN110172489A - The preparation method and application of the soluble extracellular polymeric of bacillus micro-organism - Google Patents
The preparation method and application of the soluble extracellular polymeric of bacillus micro-organism Download PDFInfo
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- CN110172489A CN110172489A CN201910357924.XA CN201910357924A CN110172489A CN 110172489 A CN110172489 A CN 110172489A CN 201910357924 A CN201910357924 A CN 201910357924A CN 110172489 A CN110172489 A CN 110172489A
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- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 65
- 244000005700 microbiome Species 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 229920000642 polymer Polymers 0.000 claims abstract description 68
- 239000000243 solution Substances 0.000 claims abstract description 58
- 239000007788 liquid Substances 0.000 claims abstract description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 51
- 238000000855 fermentation Methods 0.000 claims abstract description 48
- 230000004151 fermentation Effects 0.000 claims abstract description 48
- 239000002609 medium Substances 0.000 claims abstract description 36
- 239000000843 powder Substances 0.000 claims abstract description 24
- 239000006228 supernatant Substances 0.000 claims abstract description 24
- 239000012153 distilled water Substances 0.000 claims abstract description 18
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 230000004913 activation Effects 0.000 claims abstract description 12
- 239000007864 aqueous solution Substances 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 235000015097 nutrients Nutrition 0.000 claims abstract description 12
- 239000007787 solid Substances 0.000 claims abstract description 12
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 238000005260 corrosion Methods 0.000 claims description 49
- 230000007797 corrosion Effects 0.000 claims description 48
- 241000193755 Bacillus cereus Species 0.000 claims description 32
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 24
- 244000063299 Bacillus subtilis Species 0.000 claims description 23
- 230000002401 inhibitory effect Effects 0.000 claims description 16
- 229910052751 metal Inorganic materials 0.000 claims description 10
- 239000002184 metal Substances 0.000 claims description 10
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 9
- 239000011575 calcium Substances 0.000 claims description 9
- 229910052791 calcium Inorganic materials 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000010612 desalination reaction Methods 0.000 claims description 5
- 150000002739 metals Chemical class 0.000 claims description 3
- 239000003112 inhibitor Substances 0.000 abstract description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 34
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 28
- 230000005764 inhibitory process Effects 0.000 description 26
- 239000010935 stainless steel Substances 0.000 description 25
- 238000004458 analytical method Methods 0.000 description 24
- 125000000524 functional group Chemical group 0.000 description 24
- 229910001868 water Inorganic materials 0.000 description 21
- 239000000126 substance Substances 0.000 description 20
- 241000894006 Bacteria Species 0.000 description 19
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- 150000001408 amides Chemical class 0.000 description 18
- 229910000019 calcium carbonate Inorganic materials 0.000 description 18
- 229910001069 Ti alloy Inorganic materials 0.000 description 17
- 150000001720 carbohydrates Chemical class 0.000 description 17
- 235000011132 calcium sulphate Nutrition 0.000 description 14
- 239000000706 filtrate Substances 0.000 description 14
- 239000013535 sea water Substances 0.000 description 14
- 241000194107 Bacillus megaterium Species 0.000 description 13
- 239000007832 Na2SO4 Substances 0.000 description 13
- 229910052938 sodium sulfate Inorganic materials 0.000 description 13
- 229910001256 stainless steel alloy Inorganic materials 0.000 description 13
- 239000008367 deionised water Substances 0.000 description 12
- 229910021641 deionized water Inorganic materials 0.000 description 12
- 108090000765 processed proteins & peptides Proteins 0.000 description 12
- 229910001220 stainless steel Inorganic materials 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 11
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 10
- 241000726221 Gemma Species 0.000 description 10
- LTIPUQSMGRSZOQ-UHFFFAOYSA-N [C].[C].[O] Chemical compound [C].[C].[O] LTIPUQSMGRSZOQ-UHFFFAOYSA-N 0.000 description 10
- 229910001424 calcium ion Inorganic materials 0.000 description 10
- 230000014759 maintenance of location Effects 0.000 description 10
- 102000039446 nucleic acids Human genes 0.000 description 10
- 108020004707 nucleic acids Proteins 0.000 description 10
- 150000007523 nucleic acids Chemical class 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 9
- 239000002253 acid Substances 0.000 description 9
- 150000001491 aromatic compounds Chemical class 0.000 description 9
- 229910021538 borax Inorganic materials 0.000 description 9
- 239000001110 calcium chloride Substances 0.000 description 9
- 229910001628 calcium chloride Inorganic materials 0.000 description 9
- 238000001125 extrusion Methods 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 229920002959 polymer blend Polymers 0.000 description 9
- 229920001184 polypeptide Polymers 0.000 description 9
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 9
- 102000004196 processed proteins & peptides Human genes 0.000 description 9
- 239000004328 sodium tetraborate Substances 0.000 description 9
- 235000010339 sodium tetraborate Nutrition 0.000 description 9
- 238000010521 absorption reaction Methods 0.000 description 8
- 239000007853 buffer solution Substances 0.000 description 8
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 8
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 8
- 238000004448 titration Methods 0.000 description 8
- 238000004566 IR spectroscopy Methods 0.000 description 7
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 230000002421 anti-septic effect Effects 0.000 description 6
- MVQBFZXBLLMXGS-UHFFFAOYSA-N chembl331220 Chemical compound C1=CC=C2C(N=NC=3C4=CC=CC=C4C=C(C=3O)C(=O)O)=C(O)C=C(S(O)(=O)=O)C2=C1 MVQBFZXBLLMXGS-UHFFFAOYSA-N 0.000 description 6
- 239000000470 constituent Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000007710 freezing Methods 0.000 description 6
- 230000008014 freezing Effects 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000003628 erosive effect Effects 0.000 description 4
- 238000005498 polishing Methods 0.000 description 4
- 229910010271 silicon carbide Inorganic materials 0.000 description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 244000137852 Petrea volubilis Species 0.000 description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 3
- 150000001350 alkyl halides Chemical class 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 3
- 229910000397 disodium phosphate Inorganic materials 0.000 description 3
- 238000004821 distillation Methods 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 125000003010 ionic group Chemical group 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- 229910052603 melanterite Inorganic materials 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 125000004430 oxygen atom Chemical group O* 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 229910001631 strontium chloride Inorganic materials 0.000 description 3
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 241001677188 Coccus viridis Species 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005258 corrosion kinetic Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- -1 esters Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000012876 topography Methods 0.000 description 2
- 241000006378 Bacillus cereus group Species 0.000 description 1
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
- 108010045403 Calcium-Binding Proteins Proteins 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000001175 calcium sulphate Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 125000004185 ester group Chemical group 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000012761 high-performance material Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000001453 impedance spectrum Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 150000002902 organometallic compounds Chemical class 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F5/00—Softening water; Preventing scale; Adding scale preventatives or scale removers to water, e.g. adding sequestering agents
- C02F5/08—Treatment of water with complexing chemicals or other solubilising agents for softening, scale prevention or scale removal, e.g. adding sequestering agents
- C02F5/10—Treatment of water with complexing chemicals or other solubilising agents for softening, scale prevention or scale removal, e.g. adding sequestering agents using organic substances
- C02F5/12—Treatment of water with complexing chemicals or other solubilising agents for softening, scale prevention or scale removal, e.g. adding sequestering agents using organic substances containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23F—NON-MECHANICAL REMOVAL OF METALLIC MATERIAL FROM SURFACE; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL; MULTI-STEP PROCESSES FOR SURFACE TREATMENT OF METALLIC MATERIAL INVOLVING AT LEAST ONE PROCESS PROVIDED FOR IN CLASS C23 AND AT LEAST ONE PROCESS COVERED BY SUBCLASS C21D OR C22F OR CLASS C25
- C23F11/00—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent
- C23F11/08—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids
- C23F11/10—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids using organic inhibitors
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/02—Non-contaminated water, e.g. for industrial water supply
- C02F2103/023—Water in cooling circuits
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/08—Corrosion inhibition
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- Water Supply & Treatment (AREA)
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Abstract
The invention discloses the preparation methods and application of the soluble extracellular polymeric of bacillus micro-organism, belong to microorganisms technical field.The pH value that the present invention adjusts Luria-Bertani fluid nutrient medium is 7.0~8.0, Luria-Bertani liquid fermentation medium is used as after high-temperature sterilization, using solid Luria-Bertani culture medium, after carrying out 24~48h of activation culture to bacillus, it is seeded to 24~36h of culture in Luria-Bertani liquid fermentation medium and obtains bacillus micro-organism fermentation liquid；Bacillus micro-organism fermentation liquid is placed in centrifugal treating under the conditions of temperature is 4~8 DEG C, standing takes supernatant liquor, and supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；Polymer solution outside extracellular soluble is freeze-dried to obtain polymer powder outside extracellular soluble；Polymer powder outside extracellular soluble is dissolved in aqueous solutions of polymers outside the extracellular soluble for being configured to preset concentration in distilled water, it is spare.Solubility extracellular polymeric of the invention is as a kind of difunctional Biologic inhibitor and antisludging agent.
Description
Technical field
The present invention relates to the preparation methods and application of the soluble extracellular polymeric of bacillus micro-organism, belong to micro- life
Object technical field.
Background technique
Fouling is puzzlement industry cooling circulator and one of the main problem that desalination plant uses.This is also
Industrial process, i.e. in production of energy a primary concern problem.Dirt is covered on metal surface and not only results in underdeposit corrosion,
And will be greatly reduced its heat transfer efficiency, the loss and waste of the energy are caused, or even bring many safety accidents.In France, with
The related non-productive expenditure of expansion scale is estimated as annual 1500000000 Euros；Same expense is about 800,000,000 dollars in Britain, in day
This about 3,000,000,000 dollars, be 9,000,000,000 dollars in the U.S.；And the annual fouling in China causes economic loss to surpass 70,000,000,000 yuan, the world Yuan Chao
Average level[4]；In addition, corrosion can not only bring great safety accident, will also result in serious economic loss, 2014 I
The corrosion totle drilling cost of state includes the loss of corrosion bring and anticorrosion investment, accounts for about the 3.34% of current year GDP, total value surpasses 2.1 ten thousand
Hundred million RMB are equivalent to the corrosion cost that each Chinese undertake more than 1555 yuan for the year.Therefore, it develops high performance material or finds
Effective method prevents from calcium carbonate and calcium sulfate from being formed and slows down metal erosion to be extremely important.Organic molecule is as corrosion inhibiter
It is most popular, most effective and most practical one of the method for solving corrosion and scale problems.However, these substances often can not
Biodegrade, non-renewable, most organic inhibitors are all toxic to easily cause environmental pollution and synthesis cost high.Therefore, it opens
The inhibitor that sending out has the ecology of the characteristics such as renewable, biodegradable, cheap and nontoxic friendly is very urgent.
Summary of the invention
The technical issues of for corrosion inhibiting and descaling agent in the prior art, the present invention propose the solubility of bacillus micro-organism
The preparation method and application of extracellular polymeric prepare difunctional biological inhibition and the antisludging agent of green, solve industry cooling and follow
Corrosion and scale problems in ring and desalination plant.Solubility extracellular polymeric of the invention is as a kind of difunctional biology
Corrosion inhibiter and antisludging agent.
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 7.0~8.0, is used as Luria- after high-temperature sterilization
Bertani liquid fermentation medium, using solid Luria-Bertani culture medium, to bacillus carry out activation culture 24~
After 48h, it is seeded to 24~36h of culture in Luria-Bertani liquid fermentation medium and obtains bacillus micro-organism fermentation
Liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 4~8 DEG C, it is quiet
It sets and takes supernatant liquor, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in the solubility that preset concentration is configured in distilled water
Extracellular polymeric aqueous solution, it is spare.
The temperature of step (1) high-temperature sterilization is 100~121 DEG C, and the high-temperature sterilization time is 30~40min.
Step (1) bacillus is bacillus cereus, bacillus megaterium or bacillus subtilis.
The molecule interception of step (2) bag filter is 10000~14000kDa.
The revolving speed of step (2) centrifugal treating is 8000~12000r/s, and the time of centrifugal treating is 20~30min.
Application of the solubility extracellular polymeric as corrosion inhibiter in metals against corrosion.
Further, it is 10~200mg/L that soluble extracellular polymeric, which inhibits the concentration of metal erosion,.
The solubility extracellular polymeric is preventing the application in calcium scale as antisludging agent.
Further, it is 100~400mg/L that soluble extracellular polymeric, which inhibits the concentration of calcium scale as antisludging agent,.
The solubility extracellular polymeric answering in cooling cycle industry and desalination plant as corrosion inhibiting and descaling agent
With.
The principle of the present invention: the soluble extracellular polymeric of bacillus micro-organism contain protein, nucleic acid, polysaccharide,
The high-molecular compounds such as esters, these compound molecules are interior to contain the electronegative function such as amino, carboxyl, hydroxyl, amide groups, ester group
Energy functional group, can be effectively adsorbed in stainless steel and titanium alloy surface, and mineralising occurs with metal ion, forms biomineralized film,
Attack of the corrosive ion to metal surface is hindered, to play the role of slowing down corrosion of metal.In addition, can in bacillus
Contain a large amount of acid function functional groups and calcium binding in dissolubility extracellular polymeric, forms stable organo-metallic compound.
Since acid function functional group and calcium ion have stronger sequestering power, binding constant is far longer than calcium ion and carbonate
With the binding constant of sulfate ion, therefore the formation of calcium carbonate and calcium sulphate crystal is prevented.
Beneficial effects of the present invention:
(1) bacillus micro-organism of the invention is widespread in nature and easily cultivates, and soluble is extracellular
Polymer extraction methods are simple, environmental protection, and at low cost；
(2) the soluble extracellular polymeric of the present invention is a kind of reproducible, green, inexpensive high molecular material,
Compared with current chemically synthesized corrosion inhibiting and descaling agent, there is no the environmental problems after use, harmless to environment and biological nontoxic, can
As green scale corrosion inhibitor；
(3) green scale corrosion inhibitor of the invention suitable for cooling cycle industry and desalination plant stainless steel and
The anti-corrosion and scale removal of its titanium alloy can effectively inhibit above-mentioned corrosion of metal and prevent the formation of calcium scale.Wherein to corrosion
Inhibition efficiency be up to 91% or more and permanently effective, 89% is up to the inhibition efficiency of calcium scale.
Detailed description of the invention
Fig. 1 is the function functional group infrared spectrogram of the soluble extracellular polymeric of 1 bacillus cereus of embodiment；
Fig. 2 is the SEM figure of influence of the soluble extracellular polymeric of 1 bacillus cereus of embodiment to Corrosion of Stainless Steel；
Fig. 3 is the function functional group infrared spectrogram of the soluble extracellular polymeric of 4 bacillus megaterium of embodiment；
Fig. 4 is that the soluble extracellular polymeric of the bacillus megaterium of 4 various concentration of embodiment inhibits calcium carbonate scale to be formed
Efficiency；
Fig. 5 is the function functional group infrared spectrogram of the soluble extracellular polymeric of 7 bacillus subtilis of embodiment；
Fig. 6 is that the soluble extracellular polymeric of the bacillus subtilis of 7 various concentration of embodiment inhibits the electricity of Corrosion of Stainless Steel
Chemical impedance spectrogram.
Specific embodiment
Invention is further described in detail combined with specific embodiments below, but protection scope of the present invention is not limited to
The content.
Soluble extracellular polymeric inhibits the application method of metal erosion as corrosion inhibiter in the embodiment of the present invention:
(1) using different grades of carborundum paper, (stainless steel or titanium alloy are polishing to light by 600# to 3000#) step by step
It is bright, remove its surface contaminants；
(2) treated stainless steel or titanium alloy sample are respectively placed in 250mL artificial seawater and are 10 added with concentration
It is observed after reacting 1~30d in the 250mL artificial seawater of the soluble extracellular polymeric of~200mg/L bacillus micro-organism
Its corrosion condition；
Soluble extracellular polymeric inhibits the application method of calcium scale as antisludging agent in the embodiment of the present invention:
CaCl is added in the soluble extracellular polymeric of bacillus micro-organism2Solution and NaHCO3The mixing of solution is molten
Reaction system A is formed in liquid, is statically placed in after reacting 15~30h in the water-bath that temperature is 20~80 DEG C, filtering, filtrate titration
Method analyzes the content of its calcium ion；Wherein the outer polymer concentration of extracellular soluble is 100~400mg/L in reaction system A；
CaCl is added in the soluble extracellular polymeric of bacillus micro-organism2Solution and Na2SO4The mixing of solution is molten
Reaction system B is formed in liquid, is statically placed in after reacting 16~48h in the water-bath that temperature is 30-70 DEG C, filtering, filtrate titration
Method analyzes the content of its calcium ion；Wherein the outer polymer concentration of extracellular soluble is 100~400mg/L in reaction system B.
Embodiment 1: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus cereus
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 7.0, is placed in high temperature under the conditions of temperature is 121 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 30min, using solid Luria-Bertani culture medium, to waxy bud
After spore bacillus carries out activation culture for 24 hours, it is seeded to culture 36h in Luria-Bertani liquid fermentation medium and obtains gemma bar
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 4 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 10000kDa, and the revolving speed of centrifugal treating is 8000r/s, and the time of centrifugal treating is 30min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
Property extracellular polymeric function functional group infrared spectrogram see Fig. 1, from fig. 1, it can be seen that poly- outside the extracellular soluble of bacillus cereus
It closes in the infrared spectroscopy of object and identifies five main peaks.The main function of the outer polymer samples of the extracellular soluble of bacillus cereus
Group derives as follows: 2960-3300cm-1(O-H, N-H group), 1620cm-1(amide I), 1400cm-1(carboxyl), 1120cm-1(C-
O-C stretching vibration).Wherein, 2960-3300cm-1Wide stretching vibration band in range is assigned to the N-H amino and carbon of protein
The O-H group of hydrate.1400cm-1Peak be the midplane extrusion peak with the carboxylic acid of ionic group.1620cm-1The suction at place
Take-up corresponds to the stretching vibration of polypeptide amide I.1120cm-1Neighbouring band is attributed to carbohydrate and aromatic compound
Carbon-oxygen-carbon stretching vibration.660cm-1The adsorption peak at place and the stretching of alkyl halide are related.The result shows that bacillus cereus
Soluble extracellular polymeric is a kind of heavy polymer mixture of complexity, i.e. humic substance, uronic acid and nucleic acid.Due to
It contains oxygen atom in functional group's (oxygen-hydrogen, carbon-oxygen-carbon) and amide I, meets the general standard of typical corrosion inhibiter, sample gemma
The soluble extracellular polymeric of bacillus has potential antiseptic property；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare；
The soluble extracellular polymeric corrosion resistance of bacillus cereus
(1) 250mL artificial seawater simulated solution is prepared, and sterilizes and saves backup；Wherein artificial seawater composition are as follows: 17.6g/L
NaCl, 0.04g/L KBr, 0.008g/L SrCl2·6H2O, 0.08g/L NaHCO3, 0.6107g/L CaCl2·6H2O,
0.010g/L Na2HPO4·12H2O, 0.01g/L FeSO4·7H2O, 1.87g/L MgCl2·6H2O, 0.25g/L KCl,
1.47g/L Na2SO4, 0.008g/L H2BO3；
(2) 316L stainless steel or titanium alloy are polishing to light step by step with the SiC sand paper of #600 to #3000, it is clear with distillation
It washes, is sealed after dry；
(3) treated stainless steel or titanium alloy sample are respectively placed in 250mL artificial seawater and are denoted as blank group, added
Be denoted as experimental group in the 250mL artificial seawater of soluble extracellular polymeric, wherein in experimental group soluble extracellular polymeric it is dense
Degree be 10mg/L, 20mg/L, 30mg/L, 40mg/L, 50mg/L, 60mg/L, 70mg/L, 80mg/L, 120mg/L, 160mg/L,
A series of concentration of different soluble extracellular polymerics of 200mg/L；After impregnating 15d, sample is taken out to the table that them are observed with SEM
Face pattern studies its corrosion kinetics with electrochemical process；
Shadow of the soluble extracellular polymeric (concentration 20mg/L) of the present embodiment bacillus cereus to Corrosion of Stainless Steel
Loud SEM figure is shown in Fig. 2, and as can be seen from Figure 2, the soluble extracellular polymeric of bacillus cereus can inhibit the spot corrosion of stainless steel；
Find that the soluble extracellular polymeric of bacillus cereus can reduce stainless steel or titanium conjunction by electrochemical process research
Gold corrosion rate it is found that the soluble extracellular polymeric of bacillus cereus the corrosion of stainless steel and titanium alloy is all had it is bright
Aobvious inhibiting effect, the concentration of soluble extracellular polymeric are 10mg/L, 20mg/L, 30mg/L, 40mg/L, 50mg/L, 60mg/
L, when 70mg/L, 80mg/L, 120mg/L, 160mg/L, 200mg/L, the inhibition efficiency of stainless steel is successively up to respectively
38.02%, 46.70%, 48.2%, 50.60%, 67.18%, 79.23%, 87.58%, 88.02%, 89.60%,
92.02%, 92.12%, 91.08%, the inhibition efficiency of titanium alloy is up to 36.12%, 45.50%, 49.21%, 54.53%,
57.08%, 69.13%, 72.23%, 78.12%, 83.68%, 87.62%, 92.12%, 90.08%；
The soluble extracellular polymeric of bacillus cereus inhibits the formation of calcium scale
It can according to " measurements of GB/T16632-2008 water treatment agent scale-inhibiting properties " calcium carbonate and Deposit of Calcium Sulfate method, research
Scale-inhibiting properties of the dissolubility extracellular polymeric to calcium carbonate and calcium sulfate；
Solution is prepared: weighing the anhydrous CaCl of 13.86g2It is dissolved in deionized water, with volumetric flask constant volume in 1L (Ca2+Meter
5.00mg/L) it is labeled as No. 1 CaCl2；Weigh 10.5g NaHCO3It is dissolved in deionized water, with volumetric flask constant volume in 1L (HCO3 -
Count 7.63mg/L)；Weigh the anhydrous CaCl of 83.25g2It is dissolved in deionized water, with volumetric flask constant volume in 1L (Ca2+Count 30mg/L) mark
It is denoted as No. 1 CaCl2；Weigh 108.5g Na2SO4It is dissolved in deionized water, with volumetric flask constant volume in 1L (SO4 2-Count 73.37mg/
L)；Weigh the borax buffer solution that 14 water Boratex of 3.8g is configured to pH=9；The EDTA standard for preparing 0.005mol/L is molten
Liquid；
The test of calcium carbonate scale inhibition:
Take 200mLH210mL1 CaCl is added in the conical flask of 500mL in O210mL borax buffer solution is added in solution,
10mL NaHCO is then slowly added3Solution is diluted with water to 250mL, is denoted as blank group.Take 200mL H2O is in 500mL's
In conical flask, No. 1 CaCl of 10mL is added2Solution, be added 2~10mL concentration be 40mg/L, 60mg/L, 80mg/L, 100mg/L,
A series of soluble extracellular polymeric of different concentration such as 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L shakes
After even, 10mL borax buffer solution is added, 10mL NaHCO is then slowly added3Solution is diluted with water to 250mL, is denoted as
Experimental group.Conical flask is rested on later in 20~80 DEG C of water-bath after 15~30h, filtered, filtrate is collected spare；Filter residue,
Pattern and constituent analysis are carried out to it after freezing dry weight measurement；
It takes 25mL distilled water in the conical flask of 250mL, 2mL sodium hydroxide is added, it is certain density that 15mL is then added
Filtrate and 3mg calcon-carboxylic acid carry out dropping point with EDTA, and record titration end-point；All experiments are 3 groups parallel；Concentration is followed successively by
The solubility of 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L
Extracellular polymeric, scale inhibition efficiency successively be up to 36.50%, 47.8%, 59.03%, 67.58%, 78.02%, 84.60%,
89.02%, 90.03%, 87.08%；
The test of calcium sulfate scale inhibition:
Take 200mLH2No. 2 CaCl of 10mL are added in the conical flask of 500mL in O2Solution and 10mL Na2SO4Solution,
It is diluted with water to 250mL, is denoted as blank group；Take 200mL H2No. 2 CaCl of 10mL are added in the conical flask of 500mL in O2Solution,
Be added be added 2~10mL concentration be 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 160mg/L, 180mg/L,
A series of soluble extracellular polymeric of the soluble extracellular polymeric of different concentration such as 220mg/L, 260mg/L then adds
Enter 10mL Na2SO4Solution is diluted with water to 250mL, is denoted as experimental group；Conical flask is rested on to 30~70 DEG C of water-bath later
In pot after 16~48h, filtering, filtrate is collected spare；Filter residue carries out pattern and constituent analysis to it after freezing dry weight measurement；
It takes 25mL distilled water in the conical flask of 250mL, 2mL sodium hydroxide is added, it is certain density that 15mL is then added
Filtrate and 3mg calcon-carboxylic acid carry out dropping point with EDTA, and record titration end-point；All experiments are 3 groups parallel.Concentration is followed successively by
The solubility of 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L
Extracellular polymeric, scale inhibition efficiency successively be up to 37.05%, 39.06%, 53.56%, 61.08%, 78.09%, 89.78%,
89.03%, 88.06%, 88.69%.
Embodiment 2: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus cereus
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 7.4, is placed in high temperature under the conditions of temperature is 110 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 35min, using solid Luria-Bertani culture medium, to waxy bud
After spore bacillus carries out activation culture 30h, it is seeded to culture 28h in Luria-Bertani liquid fermentation medium and obtains gemma bar
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 5 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 11000kDa, and the revolving speed of centrifugal treating is 10000r/s, and the time of centrifugal treating is 26min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
The function functional group infrared spectrogram of property extracellular polymeric it is found that the soluble extracellular polymeric of bacillus cereus infrared light
Five main peaks are identified in spectrum.The main functional group of the outer polymer samples of the extracellular soluble of bacillus cereus derives as follows:
2960-3300cm-1(O-H, N-H group), 1620cm-1(amide I), 1400cm-1(carboxyl), 1120cm-1(C-O-C stretches vibration
It is dynamic).Wherein, 2960-3300cm-1Wide stretching vibration band in range is assigned to the N-H amino and carbohydrate of protein
O-H group.1400cm-1Peak be the midplane extrusion peak with the carboxylic acid of ionic group.1620cm-1The absorption band at place is corresponding
In the stretching vibration of polypeptide amide I.1120cm-1Neighbouring band is attributed to carbon-oxygen-of carbohydrate and aromatic compound
Carbon stretching vibration.660cm-1The adsorption peak at place and the stretching of alkyl halide are related.The result shows that the extracellular soluble of bacillus cereus
Outer polymer is a kind of heavy polymer mixture of complexity, i.e. humic substance, uronic acid and nucleic acid.Since it is in function
Contain oxygen atom in group's (oxygen-hydrogen, carbon-oxygen-carbon) and amide I, meet the general standard of typical corrosion inhibiter, sample bacillus can
Dissolubility extracellular polymeric has potential antiseptic property；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare.
Embodiment 3: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus cereus
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 8.0, is placed in high temperature under the conditions of temperature is 100 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 40min, using solid Luria-Bertani culture medium, to waxy bud
After spore bacillus carries out activation culture 48h, it is seeded to cultivate in Luria-Bertani liquid fermentation medium and obtains gemma bar for 24 hours
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 8 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 814000kDa8, and the revolving speed of centrifugal treating is 12000r/s, and the time of centrifugal treating is 20min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
The function functional group infrared spectrogram of property extracellular polymeric it is found that the soluble extracellular polymeric of bacillus cereus infrared light
Five main peaks are identified in spectrum.The main functional group of the outer polymer samples of the extracellular soluble of bacillus cereus derives as follows:
2960―3300cm-1(O-H, N-H group), 1620cm-1(amide I), 1400cm-1(carboxyl), 1120cm-1(C-O-C stretches vibration
It is dynamic).Wherein, 2960-3300cm-1Wide stretching vibration band in range is assigned to the N-H amino and carbohydrate of protein
O-H group.1400cm-1Peak be the midplane extrusion peak with the carboxylic acid of ionic group.1620cm-1The absorption band at place is corresponding
In the stretching vibration of polypeptide amide I.1120cm-1Neighbouring band is attributed to carbon-oxygen-of carbohydrate and aromatic compound
Carbon stretching vibration.660cm-1The adsorption peak at place and the stretching of alkyl halide are related.The result shows that the extracellular soluble of bacillus cereus
Outer polymer is a kind of heavy polymer mixture of complexity, i.e. humic substance, uronic acid and nucleic acid.Since it is in function
Contain oxygen atom in group's (oxygen-hydrogen, carbon-oxygen-carbon) and amide I, meet the general standard of typical corrosion inhibiter, sample bacillus can
Dissolubility extracellular polymeric has potential antiseptic property；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare.
Embodiment 4: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus megaterium
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 7.2, is placed in high temperature under the conditions of temperature is 105 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 38min, using solid Luria-Bertani culture medium, to huge bud
After spore bacillus carries out activation culture 28h, it is seeded to culture 32h in Luria-Bertani liquid fermentation medium and obtains gemma bar
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 4 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 10000kDa, and the revolving speed of centrifugal treating is 8000r/s, and the time of centrifugal treating is 30min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
The function functional group infrared spectrogram of property extracellular polymeric is shown in Fig. 3, as can be seen from Figure 3, the soluble extracellular polymer of huge spore bacillus
3340 and 3430cm in the infrared spectroscopy of object-1Peak belongs to the amino of protein and the O-H group of carbohydrate.1410,
1460cm-1Peak be carboxylic acid midplane extrusion peak.1610cm-1The absorption band at place corresponds to the stretching vibration of polypeptide amide I.
1010cm-1Neighbouring band is attributed to carbon-oxygen-carbon stretching vibration of carbohydrate and aromatic compound.The result shows that wax
The soluble extracellular polymeric of sample bacillus is a kind of heavy polymer mixture of complexity, i.e. humic substance, alditol
Acid and nucleic acid.It contains the functional group for closing the general standard of typical corrosion inhibiter, the soluble extracellular polymeric tool of huge spore bacillus
There is potential antiseptic property；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare；
The soluble extracellular polymeric corrosion resistance of bacillus megaterium
(1) 250mL artificial seawater simulated solution is prepared, and sterilizes and saves backup；Wherein artificial seawater composition are as follows: 17.6g/L
NaCl, 0.04g/L KBr, 0.008g/L SrCl2·6H2O, 0.08g/L NaHCO3, 0.6107g/L CaCl2·6H2O,
0.010g/L Na2HPO4·12H2O, 0.01g/L FeSO4·7H2O, 1.87g/L MgCl2·6H2O, 0.25g/L KCl,
1.47g/L Na2SO4, 0.008g/L H2BO3；
(2) 316L stainless steel or titanium alloy are polishing to light step by step with the SiC sand paper of #600 to #3000, it is clear with distillation
It washes, is sealed after dry；
(3) treated stainless steel or titanium alloy sample are put in respectively in 250mL artificial seawater, and contain 10mg/
L, 20mg/L, 30mg/L, 40mg/L, 50mg/L, 60mg/L, 70mg/L, 80mg/L, 120mg/L, 160mg/L, 200mg/L mono-
After impregnating 15 days in the artificial seawater of the soluble extracellular polymeric of the different concentration of series, stainless steel or titanium alloy sample are taken out
Their surface topography is observed with SEM；With electrochemical measurement corrosion condition；Outside the extracellular soluble of the present embodiment bacillus megaterium
Polymer all has apparent inhibiting effect to the corrosion of stainless steel and titanium alloy, and the concentration of soluble extracellular polymeric is
10mg/L、20mg/L、30mg/L、40mg/L、50mg/L、60mg/L、70mg/L、80mg/L、120mg/L、160mg/L、
When 200mg/L, the inhibition efficiency of stainless steel successively respectively be up to stainless steel inhibition efficiency successively respectively be up to 32.16%,
43.50%, 49.63%, 59.31%, 68.88%, 78.63%, 84.48%, 89.62%, 92.08%, 91.12%,
91.09%, 90.20%, the inhibition efficiency of titanium alloy is up to 29.02%, 35.44%, 48.01%, 57.21%, 60.33%,
67.20%, 73.83%, 77.01%, 82.98%, 88.02%, 92.38%, 89.08%；
The soluble extracellular polymeric of bacillus megaterium inhibits the formation of calcium scale
It can according to " measurements of GB/T16632-2008 water treatment agent scale-inhibiting properties " calcium carbonate and Deposit of Calcium Sulfate method, research
Scale-inhibiting properties of the dissolubility extracellular polymeric to calcium carbonate and calcium sulfate；
Solution is prepared: weighing the anhydrous CaCl of 13.86g2It is dissolved in deionized water, with volumetric flask constant volume in 1L (Ca2+Meter
5.00mg/L) it is labeled as No. 1 CaCl2；Weigh 10.5g NaHCO3It is dissolved in deionized water, with volumetric flask constant volume in 1L (HCO3 -
Count 7.63mg/L)；Weigh the anhydrous CaCl of 83.25g2It is dissolved in deionized water, with volumetric flask constant volume in 1L (Ca2+Count 30mg/L) mark
It is denoted as No. 1 CaCl2；Weigh 108.5g Na2SO4It is dissolved in deionized water, with volumetric flask constant volume in 1L (SO4 2-Count 73.37mg/
L)；Weigh the borax buffer solution that 14 water Boratex of 3.8g is configured to pH=9；The EDTA standard for preparing 0.005mol/L is molten
Liquid；
The test of calcium carbonate scale inhibition:
Take 200mLH210mL1 CaCl is added in the conical flask of 500mL in O210mL borax buffer solution is added in solution,
10mL NaHCO is then slowly added3Solution is diluted with water to 250mL, is denoted as blank group.Take 200mL H2O is in 500mL's
In conical flask, No. 1 CaCl of 10mL is added2Solution, be added 2~10mL concentration be 40mg/L, 60mg/L, 80mg/L, 100mg/L,
A series of soluble extracellular polymeric of different concentration such as 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L
After soluble extracellular polymeric shakes up, 10mL borax buffer solution is added, 10mL NaHCO is then slowly added3Solution adds
Water is diluted to 250mL, is denoted as experimental group.Conical flask is rested on later in 20~80 DEG C of water-bath after 15~30h, is filtered,
Filtrate is collected spare；Filter residue carries out pattern and constituent analysis to it after freezing dry weight measurement；Research finds bacillus megaterium
Soluble extracellular polymeric changes the pattern of calcium carbonate；
It takes 25mL distilled water in the conical flask of 250mL, 2mL sodium hydroxide is added, filtrate and the 3mg of 15mL is then added
Calcon-carboxylic acid carries out dropping point with EDTA, and records titration end-point；All experiments are 3 groups parallel；Calculate its scale inhibition efficiency；
The efficiency chart that the soluble extracellular polymeric of the present embodiment bacillus megaterium inhibits calcium carbonate scale to be formed is shown in Fig. 4,
As can be seen from Figure 4, the soluble extracellular polymeric of bacillus megaterium can obviously inhibit the formation of calcium carbonate, and concentration is followed successively by
The solubility of 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L
Extracellular polymeric, scale inhibition efficiency successively be up to 38.65%, 42.09%, 57.92%, 63.08.08%, 77.92%,
89.04%, 88.53%, 87.26%, 87.19%；With the increase of polymer concentration outside the extracellular soluble of bacillus megaterium
The efficiency of the formation of calcium carbonate is inhibited constantly to increase；
The test of calcium sulfate scale inhibition:
Take 200mL H2No. 2 CaCl of 10mL are added in the conical flask of 500mL in O2Solution and 10mL Na2SO4Solution,
It is diluted with water to 250mL, is denoted as blank group.Take 200mL H2No. 2 CaCl of 10mL are added in the conical flask of 500mL in O2Solution,
It is 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 160mg/L, 180mg/L, 220mg/ that 2~10mL concentration, which is added,
A series of soluble extracellular polymeric of the soluble extracellular polymeric of different concentration such as L, 260mg/L, is then added 10mL
Na2SO4Solution is diluted with water to 250mL, is denoted as experimental group.Conical flask is rested on later 16 in 30-70 DEG C of water-bath~
After 48h, filtering, filtrate is collected spare；Filter residue carries out pattern and constituent analysis to it after freezing dry weight measurement；
It takes 25mL distilled water in the conical flask of 250mL, 2mL sodium hydroxide is added, filtrate and the 3mg of 15mL is then added
Calcon-carboxylic acid carries out dropping point with EDTA, and records titration end-point；All experiments are 3 groups parallel；Calculate its scale inhibition efficiency；It is huge
The soluble extracellular polymeric of bacillus to calcium sulfate formation have certain inhibiting effect, concentration be followed successively by 40mg/L,
The soluble extracellular polymer of 60mg/L, 80mg/L, 100mg/L, 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L
Object, scale inhibition efficiency successively be up to 36.71%, 40.13%, 50.26%, 60.38%, 68.99%, 76.28%, 89.26%,
87.24%, 86.45%.
Embodiment 5: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus megaterium
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 7.5, is placed in high temperature under the conditions of temperature is 110 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 35min, using solid Luria-Bertani culture medium, to huge bud
After spore bacillus carries out activation culture 32h, it is seeded to culture 26h in Luria-Bertani liquid fermentation medium and obtains gemma bar
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 5 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 12000kDa, and the revolving speed of centrifugal treating is 11000r/s, and the time of centrifugal treating is 24min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
The function functional group infrared spectrogram of property extracellular polymeric it is found that the soluble extracellular polymeric of huge spore bacillus infrared spectroscopy
In 3340 and 3430cm-1Peak belongs to the amino of protein and the O-H group of carbohydrate.1410,1460cm-1Peak be
The midplane extrusion peak of carboxylic acid.1610cm-1The absorption band at place corresponds to the stretching vibration of polypeptide amide I.1010cm-1Neighbouring item
Band is attributed to carbon-oxygen-carbon stretching vibration of carbohydrate and aromatic compound.The result shows that bacillus cereus can
Dissolubility extracellular polymeric is a kind of heavy polymer mixture of complexity, i.e. humic substance, uronic acid and nucleic acid.It contains
The functional group of the general standard of typical corrosion inhibiter is closed, the soluble extracellular polymeric of huge spore bacillus has potential anti-corrosive properties
Energy；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare.
Embodiment 6: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus megaterium
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 8.0, is placed in high temperature under the conditions of temperature is 121 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 32min, using solid Luria-Bertani culture medium, to huge bud
After spore bacillus carries out activation culture 30h, it is seeded to culture 28h in Luria-Bertani liquid fermentation medium and obtains gemma bar
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 8 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 14000kDa, and the revolving speed of centrifugal treating is 12000r/s, and the time of centrifugal treating is 20min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
The function functional group infrared spectrogram of property extracellular polymeric it is found that the soluble extracellular polymeric of huge spore bacillus infrared spectroscopy
In 3340 and 3430cm-1Peak belongs to the amino of protein and the O-H group of carbohydrate.1410,1460cm-1Peak be
The midplane extrusion peak of carboxylic acid.1610cm-1The absorption band at place corresponds to the stretching vibration of polypeptide amide I.1010cm-1Neighbouring item
Band is attributed to carbon-oxygen-carbon stretching vibration of carbohydrate and aromatic compound.The result shows that bacillus cereus can
Dissolubility extracellular polymeric is a kind of heavy polymer mixture of complexity, i.e. humic substance, uronic acid and nucleic acid.It contains
The functional group of the general standard of typical corrosion inhibiter is closed, the soluble extracellular polymeric of huge spore bacillus has potential anti-corrosive properties
Energy；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare.
Embodiment 7: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus subtilis
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 7.0, is placed in high temperature under the conditions of temperature is 115 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 36min, using solid Luria-Bertani culture medium, to withered grass bud
After spore bacillus carries out activation culture 26h, it is seeded to culture 32h in Luria-Bertani liquid fermentation medium and obtains gemma bar
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 5 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 13000kDa, and the revolving speed of centrifugal treating is 9000r/s, and the time of centrifugal treating is 30min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
The function functional group infrared spectrogram of property extracellular polymeric is shown in Fig. 5, as can be seen from Figure 5, the soluble extracellular polymer of bacillus subtilis
3320 peak belongs to the amino of protein and the O-H group of carbohydrate in the infrared spectroscopy of object.1400cm-1Peak be carboxylic
The midplane extrusion peak of acid.1640cm-1, 1530cm-1The absorption band at place corresponds to polypeptide amide I and peptide amide II.1070cm-1It is attached
Close band is attributed to carbon-oxygen-carbon stretching vibration of carbohydrate and aromatic compound.The result shows that bacillus subtilis
The soluble extracellular polymeric of bacterium is a kind of heavy polymer mixture (humic substance, nucleic acid etc.) of complexity；It contains
The functional group of the general standard of typical corrosion inhibiter is closed, therefore, the soluble extracellular polymeric of bacillus subtilis has potential anti-
Rotten performance；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare；
The soluble extracellular polymeric corrosion resistance of bacillus subtilis
(1) 250mL artificial seawater simulated solution is prepared, and sterilizes and saves backup；Wherein artificial seawater composition are as follows: 17.6g/L
NaCl, 0.04g/L KBr, 0.008g/L SrCl2·6H2O, 0.08g/L NaHCO3, 0.6107g/L CaCl2·6H2O,
0.010g/L Na2HPO4·12H2O, 0.01g/L FeSO4·7H2O, 1.87g/L MgCl2·6H2O, 0.25g/L KCl,
1.47g/L Na2SO4, 0.008g/L H2BO3；
(2) 316L stainless steel or titanium alloy are polishing to light step by step with the SiC sand paper of #600 to #3000, it is clear with distillation
It washes, is sealed after dry；
(3) treated stainless steel or titanium alloy sample are respectively placed in 250mL artificial seawater and are denoted as blank group, added
It is denoted as experimental group in the 250mL artificial seawater of soluble extracellular polymeric, wherein contains 10mg/L, 20mg/L in experimental group,
30mg/L, 40mg/L, 50mg/L, 60mg/L, 70mg/L, 80mg/L, 120mg/L, 160mg/L, 200mg/L are a series of different
The soluble extracellular polymeric of concentration；After impregnating 15d, sample is taken out to the surface topography for observing them with SEM, uses electrochemical process
Study its corrosion kinetics；The soluble extracellular polymeric of the bacillus subtilis of various concentration inhibits the electrochemistry of Corrosion of Stainless Steel
Impedance spectra is shown in Fig. 6, as can be seen from Figure 6, the point of the soluble extracellular polymeric of the present embodiment bacillus subtilis to two kinds of metals
Erosion has good inhibiting effect；Apparent inhibiting effect is all had to the corrosion of stainless steel and titanium alloy, is gathered outside extracellular soluble
Close object concentration be 10mg/L, 20mg/L, 30mg/L, 40mg/L, 50mg/L, 60mg/L, 70mg/L, 80mg/L, 120mg/L,
When 160mg/L, 200mg/L, the inhibition efficiency of stainless steel successively respectively be up to 40.06%, 43.05%, 56.09%,
60.38%, 78.98%, 80.36%, 92.62%, 92.03%, 92.32%, 91.86%, 91.78%, the inhibition of titanium alloy
Efficiency is up to 39.09%, 41.66%, 53.28%, 69.78%, 72.09%, 79.32%, 86.32%, 92.54%,
91.94%, 91.02%, 89.03%；The soluble extracellular polymeric of bacillus subtilis can obviously inhibit the corrosion of stainless steel,
And as the increase resistance value of polymer concentration outside the extracellular soluble of bacillus subtilis constantly increases, inhibit the effect of corrosion
Rate constantly increases；
The soluble extracellular polymeric of bacillus subtilis inhibits the formation of calcium scale
It can according to " measurements of GB/T16632-2008 water treatment agent scale-inhibiting properties " calcium carbonate and Deposit of Calcium Sulfate method, research
Scale-inhibiting properties of the dissolubility extracellular polymeric to calcium carbonate and calcium sulfate；
Solution is prepared: weighing the anhydrous CaCl of 13.86g2It is dissolved in deionized water, with volumetric flask constant volume in 1L (Ca2+Meter
5.00mg/L) it is labeled as No. 1 CaCl2；Weigh 10.5g NaHCO3It is dissolved in deionized water, with volumetric flask constant volume in 1L (HCO3 -
Count 7.63mg/L)；Weigh the anhydrous CaCl of 83.25g2It is dissolved in deionized water, with volumetric flask constant volume in 1L (Ca2+Count 30mg/L) mark
It is denoted as No. 1 CaCl2；Weigh 108.5g Na2SO4It is dissolved in deionized water, with volumetric flask constant volume in 1L (SO4 2-Count 73.37mg/
L)；It weighs 14 water Boratex of 3.8g and is configured to the borax buffer solution that pH is 9；The EDTA standard for preparing 0.005mol/L is molten
Liquid；
The test of calcium carbonate scale inhibition:
Take 200mL H2No. 1 CaCl of 10mL is added in the conical flask of 500mL in O2It is molten that 10mL borax buffering is added in solution
10mL NaHCO is then slowly added in liquid3Solution is diluted with water to 250mL, is denoted as blank group.Take 200mL H2O is in 500mL
Conical flask in, be added No. 1 CaCl of 10mL2Solution, it is 40mg/L, 60mg/L, 80mg/L, 100mg/ that 2~10mL concentration, which is added,
A series of soluble extracellular polymeric of different concentration such as L, 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L
Soluble extracellular polymeric shake up after, be added 10mL borax buffer solution, then slowly be added 10mL NaHCO3Solution,
It is diluted with water to 250mL, is denoted as experimental group；Conical flask is rested on later in 20~80 DEG C of water-bath after 15~30h, mistake
Filter, filtrate are collected spare；Filter residue carries out pattern and constituent analysis to it after freezing dry weight measurement；
It takes 25mL distilled water in the conical flask of 250mL, 2mL sodium hydroxide is added, filtrate and the 3mg of 15mL is then added
Calcon-carboxylic acid carries out dropping point with EDTA, and records titration end-point, and all experiments are 3 groups parallel, calculates its scale inhibition efficiency；Withered grass
The soluble extracellular polymeric of bacillus to calcium sulfate formation have certain inhibiting effect, concentration be followed successively by 40mg/L,
The soluble extracellular polymer of 60mg/L, 80mg/L, 100mg/L, 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L
Object, scale inhibition efficiency successively be up to 40.25%, 51.03%, 59.89%, 68.74%, 75.06%, 89.32%, 89.06%,
88.25%, 88.03%；
The test of calcium sulfate scale inhibition:
Take 200mL H2No. 2 CaCl of 10mL are added in the conical flask of 500mL in O2Solution and 10mL Na2SO4Solution,
It is diluted with water to 250mL, is denoted as blank group；Take 200mL H2No. 2 CaCl of 10mL are added in the conical flask of 500mL in O2Solution,
It is 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 160mg/L, 180mg/L, 220mg/ that 2~10mL concentration, which is added,
A series of soluble extracellular polymeric of the soluble extracellular polymeric of different concentration such as L, 260mg/L, is then added 10mL
Na2SO4Solution is diluted with water to 250mL, is denoted as experimental group；Conical flask is rested on into the water-bath that temperature is 30~70 DEG C later
In after 16~48h, filtering, filtrate is collected spare；Filter residue carries out pattern and constituent analysis to it after freezing dry weight measurement；Research
It was found that the soluble extracellular polymeric of bacillus subtilis changes the pattern of calcium carbonate；
It takes 25mL distilled water in the conical flask of 250mL, 2mL sodium hydroxide is added, filtrate and the 3mg of 15mL is then added
Calcon-carboxylic acid carries out dropping point with EDTA, and records titration end-point；All experiments are 3 groups parallel；Calculate its scale inhibition efficiency；Withered grass
The soluble extracellular polymeric of bacillus can inhibit the formation of calcium sulfate, concentration be followed successively by 40mg/L, 60mg/L, 80mg/L,
The soluble extracellular polymeric of 100mg/L, 120mg/L, 160mg/L, 180mg/L, 220mg/L, 260mg/L, scale inhibition efficiency according to
It is secondary up to 34.02%, 38.99%, 49.08%, 60.23%, 72.89%, 89.60%, 86.03%, 89.01%,
84.06%.
Embodiment 8: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus subtilis
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 7.5, is placed in high temperature under the conditions of temperature is 100 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 40min, using solid Luria-Bertani culture medium, to withered grass bud
After spore bacillus carries out activation culture 30h, it is seeded to culture 28h in Luria-Bertani liquid fermentation medium and obtains gemma bar
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 8 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 10000kDa, and the revolving speed of centrifugal treating is 11000r/s, and the time of centrifugal treating is 25min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
The function functional group infrared spectrogram of property extracellular polymeric it is found that the soluble extracellular polymeric of bacillus subtilis infrared spectroscopy
In 3320 peak belong to the amino of protein and the O-H group of carbohydrate.1400cm-1Peak be carboxylic acid midplane extrusion
Peak.1640cm-1, 1530cm-1The absorption band at place corresponds to polypeptide amide I and peptide amide II.1070cm-1Neighbouring band attribution
In carbon-oxygen-carbon stretching vibration of carbohydrate and aromatic compound.The result shows that the extracellular soluble of bacillus subtilis
Outer polymer is a kind of heavy polymer mixture (humic substance, nucleic acid etc.) of complexity；It contains the typical corrosion inhibiter of conjunction
General standard functional group, therefore, the soluble extracellular polymeric of bacillus subtilis has potential antiseptic property；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare.
Embodiment 9: the present embodiment is the preparation and application of the soluble extracellular polymeric of bacillus subtilis
The preparation method of the soluble extracellular polymeric of bacillus micro-organism, the specific steps are as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 8.0, is placed in high temperature under the conditions of temperature is 121 DEG C and goes out
Luria-Bertani liquid fermentation medium is used as after bacterium 30min, using solid Luria-Bertani culture medium, to withered grass bud
After spore bacillus carries out activation culture 38h, it is seeded to cultivate in Luria-Bertani liquid fermentation medium and obtains gemma bar for 24 hours
Bacterium microorganism fermentation liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 6 DEG C, stood
Supernatant liquor is taken, supernatant liquor is dialysed through bag filter up to polymer solution outside extracellular soluble；The wherein molecule retention of bag filter
Amount is 14000kDa, and the revolving speed of centrifugal treating is 8000r/s, and the time of centrifugal treating is 30min；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；It adopts
With the chemical analysis of ftir analysis solubility extracellular polymeric；The present embodiment bacillus cereus it is solvable
The function functional group infrared spectrogram of property extracellular polymeric it is found that the soluble extracellular polymeric of bacillus subtilis infrared spectroscopy
In 3320 peak belong to the amino of protein and the O-H group of carbohydrate.1400cm-1Peak be carboxylic acid midplane extrusion
Peak.1640cm-1, 1530cm-1The absorption band at place corresponds to polypeptide amide I and peptide amide II.1070cm-1Neighbouring band attribution
In carbon-oxygen-carbon stretching vibration of carbohydrate and aromatic compound.The result shows that the extracellular soluble of bacillus subtilis
Outer polymer is a kind of heavy polymer mixture (humic substance, nucleic acid etc.) of complexity；It contains the typical corrosion inhibiter of conjunction
General standard functional group, therefore, the soluble extracellular polymeric of bacillus subtilis has potential antiseptic property；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in be configured in distilled water concentration be 100mg/L can
Dissolubility extracellular polymeric aqueous solution, it is spare.
Claims (7)
1. the preparation method of the soluble extracellular polymeric of bacillus micro-organism, which is characterized in that specific step is as follows:
(1) pH value for adjusting Luria-Bertani fluid nutrient medium is 7.0~8.0, is used as Luria- after high-temperature sterilization
Bertani liquid fermentation medium, using solid Luria-Bertani culture medium, to bacillus carry out activation culture 24~
After 48h, it is seeded to 24~36h of culture in Luria-Bertani liquid fermentation medium and obtains bacillus micro-organism fermentation
Liquid；
(2) the bacillus micro-organism fermentation liquid of step (1) is placed in centrifugal treating under the conditions of temperature is 4~8 DEG C, standing takes
Supernatant liquor, supernatant liquor are dialysed through bag filter up to polymer solution outside extracellular soluble；
(3) the outer polymer solution of the extracellular soluble of step (2) is freeze-dried to obtain the outer polymer powder of extracellular soluble；
(4) the outer polymer powder of step (3) extracellular soluble is dissolved in outside the extracellular soluble for being configured to preset concentration in distilled water
Aqueous solutions of polymers, it is spare.
2. preparation method according to claim 1, it is characterised in that: the temperature of step (1) high-temperature sterilization is 100~121 DEG C,
The high-temperature sterilization time is 30~40min.
3. preparation method according to claim 1, it is characterised in that: step (1) bacillus is bacillus cereus, huge
Bacillus or bacillus subtilis.
4. any one preparation method according to claim 1~3, it is characterised in that: the molecule of step (2) bag filter retains
Amount is 10000~14000kDa.
5. soluble extracellular polymeric is as corrosion inhibiter in metals against corrosion prepared by preparation method described in claim 1
Using.
6. soluble extracellular polymeric prepared by preparation method described in claim 1 is preventing answering in calcium scale as antisludging agent
With.
7. soluble extracellular polymeric is as corrosion inhibiting and descaling agent in cooling cycle work prepared by preparation method described in claim 1
Application in industry and desalination plant.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110759454A (en) * | 2019-11-29 | 2020-02-07 | 云南大学 | Method for recycling and utilizing metal resources by using bacillus extracellular polymeric substances |
| CN112760266A (en) * | 2021-02-09 | 2021-05-07 | 黑龙江大学 | Bacillus preparation for hydraulic concrete and preparation method and application thereof |
| CN113097507A (en) * | 2021-04-20 | 2021-07-09 | 云南大学 | Metal nano-particle and preparation method and application thereof |
| WO2022116598A1 (en) * | 2020-12-04 | 2022-06-09 | 东南大学 | Steel corrosion prevention method employing microbial technology |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007044439A2 (en) * | 2005-10-05 | 2007-04-19 | Acillix Incorporated | Microbial exopolymers useful for water demineralization |
| EP1792876A1 (en) * | 2005-12-02 | 2007-06-06 | Tung Hai Biotechnology Corporation | Use of gamma-polyglutamic compounds for removing heavy metals or scale in aqueous systems |
| US20070125978A1 (en) * | 2005-12-02 | 2007-06-07 | Tung Hai Biotechnology Corporation | Method for removing heavy metals from and dissolving calcium and/or magnesium scales in water or aqueous system |
| CN104071890A (en) * | 2014-06-27 | 2014-10-01 | 重庆大学 | Method for removing calcium carbonate scales by using microbial extracellular carbonic anhydrase |
| US9034436B1 (en) * | 2010-09-30 | 2015-05-19 | The United States Of America, As Represented By The Secretary Of Agriculture | Anti-corrosion coating utilizing bacterial precipitated exopolysaccharides |
| CN109321501A (en) * | 2018-10-18 | 2019-02-12 | 浙江大学 | A kind of bacillus genus bacterial strain and its application for producing after-sun recovery and acting on polysaccharide |
-
2019
- 2019-04-30 CN CN201910357924.XA patent/CN110172489A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007044439A2 (en) * | 2005-10-05 | 2007-04-19 | Acillix Incorporated | Microbial exopolymers useful for water demineralization |
| EP1792876A1 (en) * | 2005-12-02 | 2007-06-06 | Tung Hai Biotechnology Corporation | Use of gamma-polyglutamic compounds for removing heavy metals or scale in aqueous systems |
| US20070125978A1 (en) * | 2005-12-02 | 2007-06-07 | Tung Hai Biotechnology Corporation | Method for removing heavy metals from and dissolving calcium and/or magnesium scales in water or aqueous system |
| US9034436B1 (en) * | 2010-09-30 | 2015-05-19 | The United States Of America, As Represented By The Secretary Of Agriculture | Anti-corrosion coating utilizing bacterial precipitated exopolysaccharides |
| CN104071890A (en) * | 2014-06-27 | 2014-10-01 | 重庆大学 | Method for removing calcium carbonate scales by using microbial extracellular carbonic anhydrase |
| CN109321501A (en) * | 2018-10-18 | 2019-02-12 | 浙江大学 | A kind of bacillus genus bacterial strain and its application for producing after-sun recovery and acting on polysaccharide |
Non-Patent Citations (4)
| Title |
|---|
| 张万友等: "循环冷却水阻垢缓蚀的研究进展", 《硅酸盐通报》 * |
| 张万友等: "循环冷却水阻垢缓蚀的研究进展", 《硅酸盐通报》, no. 09, 15 September 2015 (2015-09-15), pages 3 * |
| 李学龙等: "蜡样芽胞杆菌菌体及其胞外聚合物对Cd~(2+)的吸附作用", 《江苏农业学报》 * |
| 李学龙等: "蜡样芽胞杆菌菌体及其胞外聚合物对Cd~(2+)的吸附作用", 《江苏农业学报》, no. 02, 4 May 2018 (2018-05-04) * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110759454A (en) * | 2019-11-29 | 2020-02-07 | 云南大学 | Method for recycling and utilizing metal resources by using bacillus extracellular polymeric substances |
| WO2022116598A1 (en) * | 2020-12-04 | 2022-06-09 | 东南大学 | Steel corrosion prevention method employing microbial technology |
| CN112760266A (en) * | 2021-02-09 | 2021-05-07 | 黑龙江大学 | Bacillus preparation for hydraulic concrete and preparation method and application thereof |
| CN112760266B (en) * | 2021-02-09 | 2022-11-25 | 黑龙江大学 | Bacillus preparation for hydraulic concrete and preparation method and application thereof |
| CN113097507A (en) * | 2021-04-20 | 2021-07-09 | 云南大学 | Metal nano-particle and preparation method and application thereof |
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