CN110161028A - Utilize the method for the perchlorate in DLLME and scanner digital image analysis measurement food - Google Patents
Utilize the method for the perchlorate in DLLME and scanner digital image analysis measurement food Download PDFInfo
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- CN110161028A CN110161028A CN201910513837.9A CN201910513837A CN110161028A CN 110161028 A CN110161028 A CN 110161028A CN 201910513837 A CN201910513837 A CN 201910513837A CN 110161028 A CN110161028 A CN 110161028A
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Abstract
The invention discloses a kind of methods using the perchlorate in scanner digital image analysis measurement water sample and food, and in the acid system of pH=4.5, methylene blue (MB) color developing agent can be with ClO4 ‑React the hydrophobic green ionic associate generated；Methanol is reused as dispersing agent, methylene chloride makees extractant, and coloured ion associated matter is enriched in organic phase methylene chloride using DLLME technology；Then the colored complex in organic phase is sampled, point sample is on Alhue paper, it is scanned after through household scanners, then grayscale mode is converted images into using GIMP and ImgeJ, selection gray scale surface area is integrated, integral optical density is found out, by handling data, establishes integral optical density and ClO4 ‑The quantitative relationship of concentration.The present invention uses acquisition color quantification data of the Alhue paper as fast eolor base bottom, and image capture operation is influenced very small by environment light, has quick, accurate, sensitive, is mainly used for ClO in water sample and food4 ‑Detection.
Description
Technical field
The present invention relates to a kind of rapid assay methods of perchlorate more particularly to a kind of utilization DLLME technology and scannings
The method that instrument Digital image analysis technique combines measurement water sample and perchlorate's content in food, belongs to food inspection technology
Field.
Background technique
Perchlorate is seen as due to its special chemistry, physical property such as highly-water-soluble, mobility and stability
A kind of novel persistence organic pollutant, main pollution source can be divided into two major classes, artificial aspect and natural aspect.Artificially
The perchlorate compounds of generation are typically derived from military and chemical products, including solid propellant, rocket fuel, ammunition, cigarette
Fire, business explosive and safe automobile air bag etc..In environmental water sample and food also containing micro perchlorate, especially beverage,
In drinking water and food.Drinking water and food are the main source and approach of human contact's perchlorate.Other frequent origins have
Milk and vegetables.Since perchlorate can be stable in the presence of in surface water and groundwater, thus pollutes and may continue
For a long time.The intracorporal perchlorate of people can generate adverse effect to health, damage the generation of thyroid hormone, Reverse transcriptase
The capture of iodine increases the impaired risk of neurodevelopment.Therefore, highly sensitive, high selection is carried out to perchlorate in environment and food
Property monitoring and analysis it is particularly important.
The method of common detection perchlorate has minor effect genes, potentiometry, gravimetric method, atomic absorption spectrography (AAS), red
External spectrum method, X-ray fluorescence method, the chromatography of ions (IC method), ion chromatography-conductance detection (IC-CD), ion chromatography
Series connection triple quadrupole bar mass spectrography (IC-MS/MS method) and gas chromatography-mass spectrography (GC-MS).But x-ray fluorescence analysis
The order of magnitude of μ g/L is not achieved in the detection of method；IC fado is suitable for the simple sample of matrix (such as water), complicated for matrix
Sample cannot achieve accurate detection vulnerable to matrix interference；The case where selectivity of IC-CD method is poor, is easy to appear false positive；
For IC-MS/MS method, since the mobile phase of IC is generally highly basic, there is the problems such as mismatching with MS/MS, mass spectrum easy to pollute is simultaneously
Influence testing result；And GC-MS method is related to multistep reaction, complicated operation.Therefore, develop it is a kind of it is quick, accurate, low at
The detection method of this perchlorate very significant, especially in short supply in resources supplIes laboratory and area.
Computer visualization analytic approach and digital picture colorimetric analysis are combined, powerful and low in cost.Using common
Electronic equipment, have as smart phone or flat bed scanner carry out biomedical imaging and sensor than traditional microscopy
Many advantages.It is compared with the traditional method, the advantage that scanner method has required reagent few, highly-safe and inexpensive.It is some normal
Enrichment method such as cloud point extraction (CPE) and dispersive liquid-liquid microextraction (DLLME) can be convenient in conjunction with scanner, application
In the quantitative analysis of many samples.Digital photographing and scan image are quantified commonly using RGB color system, but at present still
Have no that it should be in the detection of perchlorate.
Summary of the invention
DLLME technology and scanner digital image analysis measurement water sample and food are utilized the object of the present invention is to provide a kind of
In perchlorate's content method.
The present invention using methylene blue (MB) as color developing agent, in the acid system of pH=4.5 with ClO4 -What reaction generated dredges
The green ionic associate of water；Methanol is reused as dispersing agent, methylene chloride makees extractant, utilizes dispersion liquid-liquid micro-extraction
(DLLME) coloured ion associated matter is enriched in organic phase methylene chloride by technology；Then to the colored complex in organic phase
It is sampled, point sample scans after through household scanners on Alhue paper, is then turned image using GIMP and ImgeJ
It changes grayscale mode into, using ImgeJ software gel analysis function, selects gray scale sample spot surface area to be integrated, find out integral
Optical density I establishes the quantitative relationship of integral optical density I Yu ClO4- concentration by handling data.Specific method is done in detail below
Thin description.
One, ClO4 -Working curve drafting
The preparation of perchlorate standard solution: perchlorate standard substance concentration is 1.000 ± 0.001mg/mL: after dilution directly
Measurement.
The ClO of different volumes is added in 15mL centrifuge tube respectively4 -Standard solution, 0.4mL 0.10gL-1Methylenum careuleum
Solution, 1.0mL HAc-NaAc buffer solution (pH=4.5), are settled to 10mL.By 600 μ L methylene chloride-methanols (v:v=2:1)
Solution is rapidly injected in centrifuge tube by micro syringe, to induce the formation of turbid solution, and by mixture in vortex oscillation
Device acutely vibrates 2min；It is centrifuged 5min at 4000rpm using centrifuge, removes upper strata aqueous phase；4.0 μ L are taken with microsyringe
Phase point is enriched with to scan the sample spot scraps of paper using scanner after Alhue paper, solvent natural air drying, then using GIMP and
Scan image is converted into grayscale mode by ImgeJ software, and is R, G, B triple channel degree grayscale mode by picture breakdown；Choosing
It selects gray scale sample spot surface area to be integrated, finds out long-pending optical density I；With the product optical density I of various concentration standard solution to corresponding
ClO4 -Concentration is modeled, and ClO is obtained4 -The quantitative relationship of the product optical density I of concentration and standard solution.As a result, it has been found that ClO4 -
Concentration is in 10 ~ 900 μ gL-1, equation of linear regression in a linear relationship with solution integral optical density optical density I in range are as follows:
I =10.29 C μg·L-1 + 0.05 (R2 = 0.9988)
I --- integral optical density
C——ClO4 -Concentration: unit: μ gL-1。
Two, ClO in sample4 -The measurement of concentration
1、ClO4 -The measurement of concentration
0.5 ~ 6mL sample solution is sequentially added in 15mL centrifuge tube, and 0.4mL 0.10gL is added-1Methylene blue solution,
Then 1.0mL HAc-NaAc buffer solution (pH=4.5) are added, are settled to 10mL.By 600 μ L methylene chloride-methanols (v:v=2:
1) solution is rapidly injected in centrifuge tube by micro syringe, to induce the formation of turbid solution, and mixture is shaken in being vortexed
It swings device and acutely vibrates 5 min；It is centrifuged 5min at 4000rpm using centrifuge, removes upper strata aqueous phase；It is taken with microsyringe
4.0 μ L are enriched with phase point and are scanned the sample spot scraps of paper using scanner after Alhue paper, solvent natural air drying, and GIMP is then used
Scan image is converted into grayscale mode with ImgeJ software, using ImgeJ software gel analysis function, selects gray scale sample
Spot surface area is integrated, and integral optical density I is found out, and product optical density I is brought into the equation of linear regression of standard curve, is obtained
The ClO into sample solution4 -Concentration.
The detection of the above method limits (LOD)=7.94 μ gL-1, quantitative limit (LOQ)=26.5 μ gL-1；Select 100 Hes
300 μg·L-1 ClO4 -It is investigated respectively in a few days and day to day precision, relative standard deviation RSD is respectively 2.42 ~ 6.98%
With 2.68 ~ 7.21%.The result shows that this method has preferable reproducibility and stability.
2, interfering ion
Under optimum experimental condition, part common ion is had chosen to ClO4 -For 200 μ gL-1Interference experiment is carried out, when opposite
ErrorWhen ± 5%, concrete outcome is shown in Table 1.As shown in Table 1, ClO3 -The interference of ion pair measurement is relatively high, wherein ClO3 -For
Positive interference, if needing to consider pre-separation in the presence of a large amount of.
Experiment shows that in methylene blue color development system, Alhue paper not only has better color compared with TLC plate
Color reduction degree, and price is cheaper.After tested, the integral optical density on Alhue paper can at least stablize holding 8 hours
It does not change above, illustrates that color development system is with good stability.
The advantages of present invention incorporates Digital scan image analytic approach and dispersive liquid-liquid microextraction technologies, can it is quick, sensitive,
Accurately detect the content of perchlorate in water sample and food；Use Alhue paper quantitatively being counted as fast eolor base bottom
It is influenced according to, image capture operation by environment light very small, has the characteristics that instrument cost is cheap, quick, accurate, sensitive,
The quick analysis field of perchlorate in water sample and food is with high application prospect.
Detailed description of the invention
Fig. 1 is ClO4 -Working curve.
Specific embodiment
The high chlorine in water sample and food is measured to scanner digital image analysis method of the present invention below by specific embodiment
The method and detection effect of acid group are described further.
The detection of perchlorate's content in embodiment 1, milk
(1) milk sample pre-treatment: 5.0 mL milk solns are added in 50mL polypropylene conical pipe, and 5.0 mL 1.0% are added
Acetic acid aqueous solution and 20.0 mL acetonitriles.Centrifuge tube lid is covered, 5min is acutely shaken.By mixture with 6000rpm centrifugation 20
min.Supernatant is taken with the nylon syringe filter in 0.20 μm of aperture.Before measurement, the sample of all digestion is passed through into aperture 4nm
Ultrafiltration membrance filter；
(2) ClO4 -Detection: in 15mL centrifuge tube be added 0.5-6mL sample, be added 0.4mL 0.10gL-1Methylenum careuleum
Then solution is added 1.0mL HAc-NaAc buffer solution (pH=4.5), 10mL is settled to, by 600 μ L methylene chloride and methanol
(v:v=2:1) solution is rapidly injected in target analytes by micro syringe, to induce the formation of turbid solution, and will mixing
Object acutely vibrates 2min in turbula shaker.It is centrifuged 5min at 4000rpm using centrifuge, removes upper strata aqueous phase；With micro
Sample injector takes 4.0 μ L enrichment phase point to scan the sample spot scraps of paper using scanner after Alhue paper, solvent natural air drying, then
Scan image is converted into grayscale mode using GIMP and ImgeJ software, gray scale sample spot surface area is selected to be integrated,
Find out integral optical density optical density I；Integral optical density is finally substituted into above-mentioned regression equation, is calculated to be checked in prepare liquid
ClO4 -Concentration.It the results are shown in Table 2.Perchlorate concentration is 17.84 ± 0.27 μ gL-1。
The detection of perchlorate's content in embodiment 2, tap water
(1) laboratory obtains, and directly analyzes, without any pretreatment.
(2) ClO4 -Detection: in 15mL centrifuge tube be added 1-6mL sample, be added 0.4mL 0.10gL-1Methylene
Then blue solution is added 1.0mL HAc-NaAc buffer solution (pH=4.5), 10mL is settled to, by 600 μ L methylene chloride and first
Alcohol (v:v=2:1) solution is rapidly injected in target analytes by micro syringe, to induce the formation of turbid solution, and will be mixed
It closes object and acutely vibrates 5 min in turbula shaker.It is centrifuged 5min at 4000rpm using centrifuge, removes upper strata aqueous phase；With micro-
Amount sample injector takes 4.0 μ L enrichment phase point to scan the sample spot scraps of paper using scanner after Alhue paper, solvent natural air drying, so
Scan image is converted into grayscale mode using GIMP and ImgeJ software afterwards, gray scale sample spot surface area is selected to be accumulated
Point, find out integral optical density I；Integral optical density I is finally substituted into above-mentioned regression equation, ClO to be checked in prepare liquid is calculated4 -
Concentration.It the results are shown in Table 2.Perchlorate concentration is 9.89 ± 1.02 μ gL-1。
The detection of perchlorate's content in embodiment 3, spinach
(1) sample pre-treatments: sample is dry at a temperature of 120, fine powder shape is ground into mortar, mesh number is 80 sievings.It will
10.0g dry spinach sample is put into 100mL conical flask, and 30mL concentrated nitric acid is added, and room temperature is cleared up 20 hours.Followed by it is added
10mL concentrated nitric acid and hydrogen peroxide are added dropwise, and are heated on electric hot plate with 150 DEG C, catch up with most nitric acid.The process is repeated, until solution is saturating
It is bright.It is cooling to solution, secondary water constant volume is added.
(2) ClO4 -Detection: in 15mL centrifuge tube be added 0.5-6mL sample, be added 0.4mL 0.10gL-1Asia
First indigo plant solution, then be added 1.0mL HAc-NaAc buffer solution (pH=4.5), be settled to 10mL, by 600 μ L methylene chloride with
Methanol (v:v=2:1) solution is rapidly injected in target analytes by micro syringe, to induce the formation of turbid solution, and will
Mixture acutely vibrates 5 min in turbula shaker.It is centrifuged 5min at 4000rpm using centrifuge, removes upper strata aqueous phase；With
Microsyringe takes 4.0 μ L enrichment phase point to scan the sample spot scraps of paper using scanner after Alhue paper, solvent natural air drying,
Then scan image is converted into grayscale mode using GIMP and ImgeJ software, gray scale sample spot surface area is selected to carry out
Integral, finds out integral optical density I；Integral optical density is finally substituted into above-mentioned regression equation, is calculated to be checked in prepare liquid
ClO4 -Concentration.It the results are shown in Table 2.Perchlorate concentration is 98.78 ± 0.15 μ gL-1。
The detection of perchlorate's content in embodiment 4, volume core white
(1) sample pre-treatments: sample is dry at a temperature of 120, fine powder shape is ground into mortar, mesh number is 80 sievings.It will
The dry cabbages leaves of 10.0g are put into 100mL conical flask, and 30mL concentrated nitric acid is added, and room temperature is cleared up 20 hours.Followed by it is added
10mL concentrated nitric acid and hydrogen peroxide are added dropwise, and are heated on electric hot plate with 150 DEG C, catch up with most nitric acid.The process is repeated, until solution is saturating
It is bright.It is cooling to solution, secondary water constant volume is added.
(2) ClO4 -Detection: in 15mL centrifuge tube be added 0.5-6mL sample, be added 0.4mL 0.10gL-1Asia
First indigo plant solution, then be added 1.0mL HAc-NaAc buffer solution (pH=4.5), be settled to 10mL, by 600 μ L methylene chloride with
Methanol (v:v=2:1) solution is rapidly injected in target analytes by micro syringe, to induce the formation of turbid solution, and will
Mixture acutely vibrates 5 min in turbula shaker.It is centrifuged 5min at 4000rpm using centrifuge, removes upper strata aqueous phase；With
Microsyringe takes 4.0 μ L enrichment phase point to scan the sample spot scraps of paper using scanner after Alhue paper, solvent natural air drying,
Then scan image is converted into grayscale mode using GIMP and ImgeJ software, gray scale sample spot surface area is selected to carry out
Integral, finds out integral optical density I；Integral optical density is finally substituted into above-mentioned regression equation, is calculated to be checked in prepare liquid
ClO4 -Concentration.It the results are shown in Table 2.Perchlorate concentration is 31.25 ± 0.15 μ gL-1。
Claims (3)
1. using the method for the perchlorate in DLLME and scanner digital image analysis measurement food being made with methylene blue
For color developing agent, in the acid system of pH=4.5 with ClO4 -React the hydrophobic green ionic associate generated；Reuse methanol
As dispersing agent, methylene chloride makees extractant, and coloured ion associated matter is enriched in organic phase methylene chloride using DLLME；
Then the colored complex in organic phase is sampled, point sample uses after scanner scanning after on Alhue paper
GIMP and ImgeJ converts images into grayscale mode, selects gray scale surface area to be integrated, it is close to find out integral optical density light
Degree establishes integral optical density and ClO by handling data4 -The quantitative relationship of concentration.
2. the method for measuring the perchlorate in food using DLLME and scanner digital image analysis as described in claim 1,
It is characterized by: sequentially adding 0.5 ~ 6mL sample solution, 0.4mL 0.10gL in 10 ~ 15mL centrifuge tube-1Methylenum careuleum it is molten
Then liquid is added 1.0mL pH=4.5HAc-NaAc buffer solution, is settled to 10mL；600 μ L dichloro methane-methanols are led to
It crosses micro syringe to be rapidly injected in centrifuge tube, and mixture is acutely vibrated into 2min in turbula shaker；Existed using centrifuge
It is centrifuged 5min under 4000rpm, removes upper strata aqueous phase；Take 4.0 μ L enrichment phase point in the inkjet printing of 3x10cm with microsyringe
On the scraps of paper, the scanner scanning sample spot scraps of paper are used after solvent natural air drying, are then decomposed into R, G, B using GIMP and scan image
Triple channel degree grayscale mode；Using ImgeJ software gel analysis function performance, gray scale sample spot surface area is selected to be integrated,
Find out integral optical density I；The equation of linear regression that integral optical density I is brought into standard curve, is calculated in sample solution
ClO4 -Concentration；
ClO4 -Concentration is in 10 ~ 900 μ gL-1In range, ClO4 -The equation of linear regression of concentration and solution integral optical density I are as follows:
I =10.29 C μg·L-1 + 0.05
I --- integral optical density
C——ClO4 -Concentration: unit: μ gL-1。
3. the method for measuring the perchlorate in food using DLLME and scanner digital image analysis as claimed in claim 2,
It is characterized by: the volume ratio of methylene chloride and methanol is 1:0.5 ~ 1:3 in methylene chloride-methanol.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110940662A (en) * | 2019-11-22 | 2020-03-31 | 中国农业科学院茶叶研究所 | Rapid visual detection method for perchlorate in water, tea soup and tea |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101852740A (en) * | 2010-05-16 | 2010-10-06 | 丁宗庆 | Colorimetric trace analysis method of solvent extraction enrichment-solid matrix imaging |
| CN103308612A (en) * | 2013-05-24 | 2013-09-18 | 淮海工学院 | Method for rapidly detecting contents of lemon yellow and sunset yellow in food |
| US9046508B1 (en) * | 2013-03-14 | 2015-06-02 | The United States Of America As Represented By The Secretary Of The Army | Simulated explosive composition |
| CN106338510A (en) * | 2016-08-30 | 2017-01-18 | 安徽鑫河清真牛羊肉加工有限公司 | Rapid detection method for lead content of halal beef and mutton |
| CN109100309A (en) * | 2018-08-16 | 2018-12-28 | 西北师范大学 | Dispersive liquid-liquid microextraction-Android mobile phone colorimetric estimation micro calcium ion method |
-
2019
- 2019-06-14 CN CN201910513837.9A patent/CN110161028A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101852740A (en) * | 2010-05-16 | 2010-10-06 | 丁宗庆 | Colorimetric trace analysis method of solvent extraction enrichment-solid matrix imaging |
| US9046508B1 (en) * | 2013-03-14 | 2015-06-02 | The United States Of America As Represented By The Secretary Of The Army | Simulated explosive composition |
| CN103308612A (en) * | 2013-05-24 | 2013-09-18 | 淮海工学院 | Method for rapidly detecting contents of lemon yellow and sunset yellow in food |
| CN106338510A (en) * | 2016-08-30 | 2017-01-18 | 安徽鑫河清真牛羊肉加工有限公司 | Rapid detection method for lead content of halal beef and mutton |
| CN109100309A (en) * | 2018-08-16 | 2018-12-28 | 西北师范大学 | Dispersive liquid-liquid microextraction-Android mobile phone colorimetric estimation micro calcium ion method |
Non-Patent Citations (2)
| Title |
|---|
| 宋正规 等: "高氯酸盐毒性及其检测方法研究进展", 《食品工业科技》 * |
| 陈国榕: "基于扫描仪和智能手机的数字图像比色法在食品和生物样品检测中的应用研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110940662A (en) * | 2019-11-22 | 2020-03-31 | 中国农业科学院茶叶研究所 | Rapid visual detection method for perchlorate in water, tea soup and tea |
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Application publication date: 20190823 |