CN110160956B - Pathological section scanning image analysis system and scanning method thereof - Google Patents

Pathological section scanning image analysis system and scanning method thereof Download PDF

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Publication number
CN110160956B
CN110160956B CN201910446975.XA CN201910446975A CN110160956B CN 110160956 B CN110160956 B CN 110160956B CN 201910446975 A CN201910446975 A CN 201910446975A CN 110160956 B CN110160956 B CN 110160956B
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slide
axis
plate
analysis system
image analysis
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CN110160956A (en
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方陕渊
张凯闻
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Beijing Intemet Technology Co ltd
Guangzhou Intemet Technology Co ltd
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Beijing Intemet Technology Co ltd
Guangzhou Intemet Technology Co ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106—General arrangement of respective parts
    • G01N2021/0112—Apparatus in one mechanical, optical or electronic block

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

The invention discloses a pathological section scanning image analysis system and a scanning method thereof, the scanning method of the pathological section scanning image analysis system is applied to a slide shooting scanning mirror of the pathological section scanning image analysis system, the slide shooting scanning mirror comprises a microscope tube and a main camera, and the scanning method comprises the following steps: the magnification of the microscope tube is reduced, so that the depth of field of the microscope tube is increased, and a larger clear imaging area is obtained; the camera shooting pixels of the main camera are improved, and higher pixel density is obtained. The scanning method of the pathological section scanning image analysis system is characterized in that the magnification factor of the microscope tube is reduced, so that the depth of field of the microscope tube is increased, the camera shooting pixels of the main camera are improved, and the pixel density and the definition degree which can be achieved by the microscope tube with the original factor are further achieved; the longer the depth of field is, the larger the corresponding clear imaging interval is, the larger the tolerance rate is, the focusing difficulty can be reduced, the focusing is rapid, and the requirement on the moving precision of a mechanical structure moving part is relatively low.

Description

Pathological section scanning image analysis system and scanning method thereof
Technical Field
The invention relates to the field of medical test equipment, in particular to a pathological section scanning image analysis system and a pathological section scanning image analysis method.
Background
At present, in order to obtain higher definition in the process of slice scanning performed by the slice scanning system, a microscope with a larger magnification is generally desired. The majority of the existing slice scanning systems use microscopes with twenty to forty times magnification, the depth of field of the microscope with twenty times magnification is generally between 1 micron and 1.4 microns according to different brands, and the slice surface is rough and unsmooth, so that the image in the field area observed by the objective lens is clear nearby and is fuzzy far away, therefore, in the slice scanning process, the mechanical focusing method of the microscope has a complex structure, the moving precision of the objective table is limited by machinery, the requirement of rapid focusing cannot be met, the focusing is easy to cause inaccurate, and the defocusing phenomenon occurs.
Therefore, it is necessary to develop a scanning method that can reduce the precision requirement for the focusing mechanism of the scanning system, reduce the focusing difficulty, and achieve fast focusing.
Disclosure of Invention
The invention aims to provide a pathological section scanning image analysis system and a scanning method thereof, wherein the scanning method of the pathological section scanning image analysis system increases the depth of field of a microscope column by reducing the magnification of the microscope column, improves the shooting pixels of a main camera and further achieves the pixel density and definition which can be achieved by the microscope column with the original magnification; the longer the depth of field is, the larger the corresponding clear imaging interval is, the larger the tolerance rate is, the focusing difficulty can be reduced, the focusing is rapid, and the requirement on the moving precision of a mechanical structure moving part is relatively low.
The technical scheme is as follows:
a scanning method of a pathological section scanning image analysis system is applied to a slide shooting scanning mirror of the pathological section scanning image analysis system, the slide shooting scanning mirror comprises a microscope tube and a main camera, and the scanning method comprises the following steps:
the magnification of the microscope tube is reduced, so that the depth of field of the microscope tube is increased, and a larger clear imaging area is obtained; the magnification of the microscope tube is six times to fourteen times;
the shooting pixels of the main camera are improved, and higher pixel density is obtained; the image pickup pixel of the main camera is greater than or equal to four hundred and fifty thousand pixels.
The microscope lens barrel comprises an eyepiece end and an objective end, and the main camera is arranged on the eyepiece end of the microscope lens barrel; the magnification of the microscope tube is eight times to twelve times, and the shooting pixels of the main camera are four hundred and fifty thousand pixels to six million pixels.
The magnification of the microscope tube is ten times, and the shooting pixels of the main camera are five million pixels.
The obtaining of the higher pixel density comprises adopting an area array picture acquisition mode, wherein the area array picture acquisition mode comprises the following steps:
firstly, a slice to be scanned is divided into a plurality of bit plane pictures with equal areas;
and synthesizing the bit plane pictures into a digital image.
The horizontal movement and the vertical focusing movement of the slide shooting scanning mirror are carried out simultaneously.
The pathological section scanning image analysis system further comprises an auxiliary camera applied to the pathological section scanning image analysis system, and the scanning method comprises the following steps:
after a slide loading mechanism is loaded with a slide with a pathological sample, moving the slide loading mechanism to the position below the slide shooting scanning mirror;
the auxiliary camera shoots the picture data of the slide, and the scanning area of the slide to be scanned is preliminarily determined according to the picture data;
and the slide shooting scanning mirror scans the pathological sample on the slide in the scanning area.
The pathological section scanning image analysis system comprises a base, a slide loading mechanism arranged on the base and a slide shooting scanning mirror arranged on the base, wherein the slide shooting scanning mirror comprises a microscope tube and a main camera, and the magnification of the microscope tube is six times to fourteen times; the image pickup pixel of the main camera is greater than or equal to four hundred and fifty thousand pixels.
The microscope lens barrel comprises an eyepiece end and an objective end, and the main camera is arranged on the eyepiece end of the microscope lens barrel; the magnification of the microscope tube is eight times to twelve times, and the shooting pixels of the main camera are four hundred and fifty thousand pixels to six million pixels.
The magnification of the microscope tube is ten times, and the shooting pixels of the main camera are five million pixels.
Pathological section scanning image analysis system still including install in the supplementary camera of base.
The base comprises an X-axis transmission mechanism and a Y-axis transmission mechanism, the Y-axis transmission mechanism is positioned above the X-axis transmission mechanism, and the transmission directions of the X-axis transmission mechanism and the Y-axis transmission mechanism are vertical to each other; the slide loading mechanism is arranged on the X-axis transmission mechanism, and the slide shooting scanning mirror is arranged on the Y-axis transmission mechanism.
The driving direction of the X-axis driving mechanism drives along the X-axis direction, the driving direction of the Y-axis driving mechanism drives along the Y-axis direction, the X-axis and the Y-axis are mutually vertical, the focusing moving direction of the slide shooting scanning mirror moves along the Z-axis direction, and the Z-axis is vertically vertical to the X-axis and the Y-axis.
The base further comprises an X-axis mounting frame, a Y-axis mounting frame and a bottom plate, the X-axis mounting frame and the Y-axis mounting frame are respectively mounted on the bottom plate, and the X-axis transmission mechanism and the Y-axis transmission mechanism are respectively mounted on the X-axis mounting frame and the Y-axis mounting frame.
The X-axis mounting frame and the Y-axis mounting frame are made of standard aluminum profiles respectively.
The X-axis mounting frame comprises an X-axis bearing front seat plate, an X-axis bearing rear seat plate and an X-axis connecting rod, wherein two ends of the X-axis connecting rod are respectively connected with the lower ends of the X-axis bearing front seat plate and the X-axis bearing rear seat plate, and the X-axis transmission mechanism is mounted on the X-axis bearing front seat plate and the X-axis bearing rear seat plate.
The X-axis transmission mechanism comprises an X-axis guide rail, an X-axis sliding block, an X-axis driving motor, an X-axis driving wheel and an X-axis transmission belt, two ends of the X-axis guide rail are respectively connected with the upper ends of the X-bearing front base plate and the X-bearing rear base plate, the X-axis sliding block is connected with the X-axis guide rail in a sliding mode, the X-axis driving wheel and the X-axis driving motor are respectively installed on the X-bearing front base plate and the X-bearing rear base plate, the X-axis driving motor is provided with an X-axis driving wheel, two ends of the X-axis transmission belt are connected with two ends of the X-axis sliding block and wound on the X-axis driving wheel and the X-axis driving wheel, and the slide loading mechanism is installed on the X-axis sliding block.
The upper ends of the X bearing front seat plate and the X bearing rear seat plate are respectively provided with a first transmission through hole and a second transmission through hole, the X-axis transmission wheel is installed on the first transmission through hole, the X-axis driving motor is installed on the outer side of the X bearing rear seat plate, one end of the X-axis transmission belt is wound on the X-axis transmission wheel, and the other end of the X-axis transmission belt penetrates through the second transmission through hole and is wound on the X-axis driving wheel.
The X-axis sliding block comprises a first sliding block, a second sliding block and a slide glass supporting plate, the X-axis guide rail comprises a first guide rail and a second guide rail, the X-axis driving motor comprises a first motor and a second motor, the X-axis driving wheel comprises a first driving wheel and a second driving wheel, the X-axis driving belt comprises a first driving belt and a second driving belt, two ends of the first guide rail and the second guide rail are respectively connected with two sides of the upper ends of the X-bearing front seat plate and the X-bearing rear seat plate, the first sliding block and the second sliding block are respectively connected with the first guide rail and the second guide rail in a sliding mode, two sides of the slide glass supporting plate are respectively connected with the first sliding block and the second sliding block, a bracket light hole is formed in the middle of the slide glass supporting plate, and the slide glass loading mechanism is installed on the slide glass supporting plate and corresponds to the position of the bracket light hole; first drive wheel, second drive wheel are installed respectively on the upper end both sides of X bearing front seat board, and first motor, second motor are installed respectively on the both sides of X bearing rear seat board, first motor, second motor have first action wheel, second action wheel respectively, the both ends of first drive belt are connected with the both ends of first slider to the winding is on first drive wheel, first action wheel, the both ends of second drive belt are connected with the both ends of second slider, and the winding is on second drive wheel, second action wheel.
The first sliding block comprises a first box-type bearing and a second box-type bearing, the second sliding block comprises a third box-type bearing and a fourth box-type bearing, the first box-type bearing and the second box-type bearing are respectively connected with two ends of one side of the slide supporting plate, and the third box-type bearing and the fourth box-type bearing are respectively connected with two ends of the other side of the slide supporting plate; the first box-type bearing, the second box-type bearing, the third box-type bearing and the fourth box-type bearing are respectively provided with a first shaft hole, a second shaft hole, a third shaft hole and a fourth shaft hole, the first guide rail penetrates through the first shaft hole and the second shaft hole and is in sliding connection with the first box-type bearing and the second box-type bearing, and the second guide rail penetrates through the third shaft hole and the fourth shaft hole and is in sliding connection with the third box-type bearing and the fourth box-type bearing.
The first box-type bearing, the second box-type bearing, the third box-type bearing and the fourth box-type bearing respectively comprise a first plastic bearing inner sleeve, a second plastic bearing inner sleeve, a third plastic bearing inner sleeve and a fourth plastic bearing inner sleeve, and the first box-type bearing, the second box-type bearing, the third box-type bearing and the fourth box-type bearing are respectively in sliding connection with the first guide rail and the second guide rail through the first plastic bearing inner sleeve, the second plastic bearing inner sleeve, the third plastic bearing inner sleeve and the fourth plastic bearing inner sleeve.
The upper end middle part of the X bearing front seat plate is provided with a first taking notch, the first taking notch corresponds to the slide loading mechanism, and the first guide rail and the second guide rail are respectively positioned on two opposite sides of the first taking notch.
The Y-axis mounting frame comprises a first portal frame, a second portal frame, a first support cross beam plate and a second support cross beam plate, the lower ends of the first portal frame and the second portal frame are respectively connected with the bottom plate, the first portal frame and the second portal frame are respectively provided with a first door opening and a second door opening, the first door opening and the second door opening are corresponding, the X-axis mounting frame penetrates through the first door opening and the second door opening, two ends of the first support cross beam plate and the second support cross beam plate are respectively connected with the upper ends of the first portal frame and the second portal frame, and the Y-axis transmission mechanism is mounted on the first support cross beam plate and the second support cross beam plate.
Y axle drive mechanism includes Y axle guide rail, Y axle sliding block, Y axle driving motor, Y axle drive wheel, Y axle drive belt, the both ends of Y axle guide rail respectively with first support crossbeam board, second support crossbeam board are connected, Y axle sliding block and Y axle guide rail sliding connection, Y axle drive wheel, Y axle driving motor are installed respectively on first support crossbeam board, the second support crossbeam board, Y axle driving motor has the Y axle action wheel, the both ends of Y axle drive belt are connected with the both ends of Y axle sliding block to the winding is on Y axle drive wheel, Y axle action wheel, the slide scanning mirror of making a video recording is installed on the Y axle sliding block.
And a third transmission through hole is formed in the first support cross beam plate, the Y-axis transmission wheel is installed on the third transmission through hole, and the Y-axis driving motor is installed on the inner side surface of the second support cross beam plate.
The Y-axis sliding block comprises a third sliding block, a fourth sliding block and a shooting bracket plate, the Y-axis guide rail comprises a third guide rail and a fourth guide rail, two ends of the third guide rail and the fourth guide rail are respectively connected with two sides of the first support cross beam plate and the second support cross beam plate, the third sliding block and the fourth sliding block are respectively connected with the third guide rail and the fourth guide rail in a sliding mode, two sides of the shooting bracket plate are respectively connected with the third sliding block and the fourth sliding block, and the slide shooting scanning mirror is installed on the shooting bracket plate.
The third sliding block comprises a fifth box-type bearing and a sixth box-type bearing, the fourth sliding block comprises a seventh box-type bearing and an eighth box-type bearing, the fifth box-type bearing and the sixth box-type bearing are respectively connected with two ends of one side of the camera bracket plate, and the seventh box-type bearing and the eighth box-type bearing are respectively connected with two ends of the other side of the camera bracket plate; the fifth box-type bearing, the sixth box-type bearing, the seventh box-type bearing and the eighth box-type bearing are respectively provided with a fifth shaft hole, a sixth shaft hole, a seventh shaft hole and an eighth shaft hole, the third guide rail penetrates through the fifth shaft hole and the sixth shaft hole and is in sliding connection with the fifth box-type bearing and the sixth box-type bearing, and the fourth guide rail penetrates through the seventh shaft hole and the eighth shaft hole and is in sliding connection with the seventh box-type bearing and the eighth box-type bearing.
The fifth box-type bearing, the sixth box-type bearing, the seventh box-type bearing and the eighth box-type bearing respectively comprise a fifth plastic bearing inner sleeve, a sixth plastic bearing inner sleeve, a seventh plastic bearing inner sleeve and an eighth plastic bearing inner sleeve, and the fifth box-type bearing, the sixth box-type bearing, the seventh box-type bearing and the eighth box-type bearing are respectively in sliding connection with the third guide rail and the fourth guide rail through the fifth plastic bearing inner sleeve, the sixth plastic bearing inner sleeve, the seventh plastic bearing inner sleeve and the eighth plastic bearing inner sleeve.
The slide shooting scanning mirror comprises a microscope tube, a main camera, a Z-axis transmission mechanism, a fixed support, an arched frame, a condensing mirror and a shooting light source, wherein the Z-axis transmission mechanism and the arched frame are arranged on the Y-axis sliding block through the fixed support; the collecting mirror and the image pickup light source are arranged on the bow-shaped frame and correspond to the microscope lens barrel, the collecting mirror is positioned above the image pickup light source, an image pickup gap is formed between the microscope lens barrel and the collecting mirror, and the slide loading mechanism penetrates through the image pickup gap.
The Z-axis transmission mechanism comprises a Z-axis driving motor, a Z-axis precise moving platform and a lead screw, the Z-axis driving motor is installed on the fixed support, the Z-axis precise moving platform is in vertical sliding connection with the fixed support, the Z-axis driving motor is in driving connection with the Z-axis precise moving platform through the lead screw, and the microscope lens cone is installed on the Z-axis precise moving platform.
The Z-axis transmission mechanism further comprises a coupler, and the lead screw is connected with the Z-axis driving motor through the coupler.
The Z-axis transmission mechanism further comprises a lens cone fixing seat, and the microscope lens cone is mounted on the Z-axis precision moving platform through the lens cone fixing seat.
The Z-axis transmission mechanism further comprises a Z-axis position feedback device, and the Z-axis position feedback device is installed on the side face of the Z-axis precision moving table.
The Y-axis sliding block is provided with a lens cone through hole and a Z-axis driving through hole, the microscope lens cone vertically penetrates through the lens cone through hole and is installed on the lens cone fixing seat, the Z-axis driving motor is located above the Y-axis sliding block, and the coupler penetrates through the Z-axis driving through hole from the lower side of the Y-axis sliding block and is connected with the Z-axis driving motor.
The bow-shaped frame includes bow-shaped support, condensing lens fixed plate, L shape bracket, the one end of bow-shaped support with the fixed bolster is connected, the other end of bow-shaped support with condensing lens fixed plate, L shape bracket, the condensing lens fixed plate is located the top of L shape bracket, install respectively condensing lens, the light source of making a video recording on condensing lens fixed plate, L shape bracket.
The camera shooting light source comprises an LED lamp and a heat dissipation plate, the LED lamp and the heat dissipation plate are respectively arranged on the L-shaped bracket, and the LED lamp is located above the heat dissipation plate.
The first portal frame comprises a first upright post, a second upright post and a first portal top beam, two ends of the first portal top beam are respectively connected with the upper ends of the first upright post and the second upright post, the light-emitting plate is mounted on the first upright post and the second upright post through the auxiliary light-emitting bottom plate support, and the light-emitting plate is positioned below the X-axis transmission mechanism; the auxiliary camera passes through the auxiliary camera fixing base is installed on the first door top beam, the direction of making a video recording of auxiliary camera is towards the luminescent plate.
The auxiliary light-emitting bottom plate support comprises an auxiliary light-emitting base and an auxiliary light-emitting bottom plate beam, two ends of the auxiliary light-emitting bottom plate beam are respectively connected with the first stand column and the second stand column, the light-emitting plate passes through the auxiliary light-emitting base and is installed on the auxiliary light-emitting bottom plate beam, and the light emitting direction of the light-emitting plate faces upwards.
The auxiliary positioning mechanism further comprises an illuminating light source, and the illuminating light source is installed on the first door top cross beam.
The luminous plate is an LED luminous plate, and the illuminating light source is an LED illuminating lamp.
The slide loading mechanism comprises a slide frame and a placing support, the slide frame passes through the placing support and is arranged on the slide supporting plate, a support light hole is formed in the middle of the placing support, and the slide frame corresponds to the support light hole and the bracket light hole.
The two sides of the placing support are respectively provided with a first positioning notch and a second positioning notch, and the first positioning notch and the second positioning notch are respectively connected with the first sliding block and the second sliding block in a matched mode.
The placing support comprises a placing bottom frame and a placing upper pressing seat, the placing bottom frame comprises a first side frame strip, a second side frame strip, a first end frame strip and a first end plate, two ends of the first end frame strip are respectively connected with one ends of the first side frame strip and the second side frame strip, two ends of the first end plate are respectively connected with the other ends of the first side frame strip and the second side frame strip, the first side frame strip, the second side frame strip, the first end frame strip and the first end plate enclose the bracket light-transmitting hole, the first side frame strip, the second side frame strip and the first end frame strip are respectively provided with a first groove, a second groove and a third groove at one side facing the light hole of the bracket, first recess, second recess, third recess and the roof face UNICOM of first end plate, it closes to place the pressure seat lid place on the chassis to form the card slot chamber, the slide frame with card slot chamber looks accordant connection.
The first groove and the second groove are respectively provided with a plurality of positioning buckles, and the slide rack is connected with the card slot cavity through the positioning buckles.
The slide frame comprises a handle and a slide bracket, wherein the handle is connected with the outer side surface of one end of the slide bracket.
The glass slide bracket comprises a glass slide frame and a plurality of glass slide separation beams, the glass slide frame is separated by the glass slide separation beams to form a plurality of glass slide placing positions, and a glass slide bottom support is arranged at the bottom end of each glass slide placing position.
The glass slide bottom supports comprise a first glass slide bottom support and a second glass slide bottom support, the first glass slide bottom support and the second glass slide bottom support are respectively located at two ends of the glass slide placing position, and the first glass slide bottom support and the second glass slide bottom support are used for supporting two ends of a glass slide.
The glass slide frame comprises a first glass slide support, a second glass slide support, a first glass slide beam and a second glass slide beam, wherein two ends of the first glass slide support and the second glass slide support are respectively connected with two ends of the first glass slide beam and two ends of the second glass slide beam, and two ends of the plurality of glass slide separating beams are respectively connected with the first glass slide support and the second glass slide support and are positioned between the first glass slide beam and the second glass slide beam; and a gap is reserved between the second slide bottom support and the second slide support, a positioning strip hole is formed, and the positioning strip hole is matched with the positioning buckle.
The number of the first slide bottom supports is two, and the two first slide bottom supports are respectively positioned on two sides of one end of the slide placing position.
And two ends of each second glass slide bottom support are respectively connected with the first glass slide beam, the second glass slide beam and the glass slide separation beam on two sides of each glass slide placing position.
The number of the slide placing positions is five, the number of the slide placing positions is five corresponding to the spring buckles, the number of the second slide bottom supports is five, and the number of the first slide bottom supports is ten.
It should be noted that:
the foregoing references to "first and second …" do not denote any particular quantity or order, but rather are used to distinguish one name from another.
The direction indicated by the aforementioned "X axis" and the direction indicated by the "Y axis" are perpendicular to each other, and the direction indicated by the aforementioned "Z axis" is perpendicular to the "X axis" and the "Y axis".
The orientation or positional relationship indicated by the "both ends of the slide placing position" mentioned above is based on the orientation or positional relationship indicated by the slide length direction when the slide is placed.
The orientation or positional relationship indicated by the "both sides of the slide placing position" is perpendicular to the orientation or positional relationship indicated by the "both ends of the slide placing position".
The orientation or positional relationship indicated by the aforementioned "both ends of the slide loading unit" is the orientation or positional relationship indicated based on the slide loading unit conveyance direction.
The orientation or positional relationship indicated by the aforementioned "both sides of the slide loading unit" is orthogonal to the orientation or positional relationship indicated by the "both ends of the slide loading unit".
The foregoing "up", "down", etc. indicate orientations or positional relationships that are based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships that are conventionally placed when the products of the present invention are used, or orientations or positional relationships that are conventionally understood by those skilled in the art, and are merely for convenience in describing and simplifying the description, but do not indicate or imply that the equipment or components that are referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
The following illustrates the advantages or principles of the invention:
1. the scanning method of the pathological section scanning image analysis system is characterized in that the magnification times of the microscope tube are reduced, so that the depth of field of the microscope tube is increased, the longer the depth of field is, the larger the corresponding clear imaging interval is, the larger the tolerance rate is, the focusing difficulty can be reduced, the focusing is rapid, and the requirement on the moving precision of a mechanical structure moving part is relatively low; the shooting pixels of the main camera are improved, so that higher pixel density can be obtained on the same target surface size, and the high-optical-resolution objective lens is matched, so that the microscope tube with the reduced magnification can reach the pixel density and the definition which can be achieved only by the microscope tube with the original magnification.
2. The magnification of the microscope tube is eight times to twelve times, the image pickup pixels of the main camera are four hundred and fifty thousand pixels to six million pixels, preferably, the magnification of the microscope tube is ten times, the shooting pixels of the main camera are five million pixels, a microscope tube commonly used by a slide scanning system on the market uses an objective lens of twenty times or forty times, the depth of field of the objective lens of twenty times magnification is generally between 1 micron and 1.4 microns according to different brands, the invention adopts ten times of objective lenses, the depth of field of the lens is 3.6 microns, the depth of field of the ten-fold objective lens is three times of that of the twenty-fold objective lens, the depth of field of the ten-fold objective lens is larger than that of the twenty-fold objective lens, therefore, the invention can obtain more focusing contents under fewer layers during scanning, has lower requirements on focusing precision, and has the advantage of lower cost due to the use of the lens with relatively small light transmission amount; the invention adopts the main camera with the shooting pixel density of five million pixels to replace a common camera with two million pixels in the market, obtains higher pixel density on the same target surface size, and is matched with the objective lens with high optical resolution, thereby achieving the pixel density and the definition degree which can be achieved by the original twenty objective lens by using ten times of objective lens.
3. The scanning method adopts an area array picture acquisition mode, and the acquisition mode of a relative line matrix is clearer in the aspect of definition.
4. The horizontal movement and the vertical focusing movement of the slide shooting scanning mirror of the scanning method are carried out simultaneously, so that the focusing movement time is further reduced.
5. The pathological section scanning image analysis system comprises a base, a slide loading mechanism and a slide shooting scanning mirror, wherein the base comprises an X-axis transmission mechanism and a Y-axis transmission mechanism, the slide shooting scanning mirror is vertically focused and horizontally moved along a Y axis, and can be simultaneously carried out;
this pathological section scanning image analysis system makes slide scanning mirror of making a video recording realize that the Z axle reciprocates to focus and the horizontal migration of Y axle goes on simultaneously, has reduced the removal time, and the effectual X axle removal separation with slide loading of slide scanning mirror is made a video recording to the slide, and the mutual noninterference that misplaces from top to bottom, slide frame remove to be difficult to the shake, and scanning height is stable, improves the scanning definition.
6. The focusing moving direction of the slide shooting scanning mirror is vertical to the X-axis transmission mechanism and the Y-axis transmission mechanism, so that the focusing moving direction of the slide shooting scanning mirror is vertical to the slide loading mechanism, and the shooting definition is improved.
7. The base still includes X axle mounting bracket, Y axle mounting bracket, bottom plate, and X axle drive mechanism, Y axle drive mechanism install respectively on X axle mounting bracket, Y axle mounting bracket, and X axle transmission and Y axle transmission are effectively separated, and mutual noninterference misplaces from top to bottom makes equipment structure overall arrangement more compact.
8. The X-axis mounting frame and the Y-axis mounting frame are made of standard aluminum profiles, so that the material cost is reduced, the assembly precision requirement is reduced, product modularization is realized, and the production efficiency is improved.
9. The X-axis mounting frame comprises an X-bearing front seat plate, an X-bearing rear seat plate and an X-axis connecting rod, and the X-axis transmission mechanism is mounted on the X-bearing front seat plate and the X-bearing rear seat plate and is convenient to mount.
10. The X-axis transmission mechanism comprises an X-axis guide rail, an X-axis sliding block, an X-axis driving motor, an X-axis transmission wheel and an X-axis transmission belt, and when the slide glass loading mechanism is used, the X-axis sliding block slides back and forth along the X-axis guide rail by starting the positive and negative rotation of the X-axis driving motor, so that the slide glass loading mechanism is driven to move along the X-axis direction.
11. The upper ends of the X bearing front seat plate and the X bearing rear seat plate are respectively provided with a first transmission through hole and a second transmission through hole, an X-axis transmission wheel is installed on the first transmission through hole, one end of an X-axis transmission belt is wound on the X-axis transmission wheel, the other end of the X-axis transmission belt penetrates through the second transmission through hole to be wound on an X-axis driving wheel, the space is saved, an X-axis driving motor is installed on the outer side of an X-axis mounting frame, and the influence on space operation in the X-axis mounting frame is avoided.
12. The X-axis sliding block comprises a first sliding block, a second sliding block and a slide supporting plate, the X-axis guide rail comprises a first guide rail and a second guide rail, and the first sliding block and the second sliding block are respectively in sliding connection with the first guide rail and the second guide rail, so that the X-axis sliding block can slide more stably on the X-axis guide rail.
13. The first sliding block and the second sliding block respectively adopt box-type bearings, and the box-type bearings are longer than common bearings, so that the moving rigidity of the X-axis sliding block is more stable, and the structural rigidity is better.
14. The first box-type bearing, the second box-type bearing, the third box-type bearing and the fourth box-type bearing are respectively in sliding connection with the first guide rail and the second guide rail through the first plastic bearing inner sleeve, the second plastic bearing inner sleeve, the third plastic bearing inner sleeve and the fourth plastic bearing inner sleeve, and therefore the moving stability of the X-axis sliding block is improved.
15. The middle part of the upper end of the X bearing front seat plate is provided with a first taking notch, so that the slide loading mechanism can be conveniently taken.
16. The Y-axis mounting frame comprises a first portal frame, a second portal frame, a first support cross beam plate and a second support cross beam plate, the X-axis mounting frame penetrates through the first door opening and the second door opening, so that the Y-axis mounting frame and the X-axis mounting frame are not interfered with each other, the Y-axis mounting frame and the X-axis mounting frame are staggered from top to bottom, the structure is more compact, the requirement on the stability of transportation is lowered, and the maintenance cost is lowered.
17. The Y-axis transmission mechanism comprises a Y-axis guide rail, a Y-axis sliding block, a Y-axis driving motor, a Y-axis transmission wheel and a Y-axis transmission belt, and when the slide shooting device is used, the Y-axis sliding block slides back and forth along the Y-axis guide rail by starting the forward and reverse rotation of the Y-axis driving motor, so that the slide shooting scanning mirror is driven to move along the Y-axis direction.
18. And a third transmission through hole is formed in the first support cross beam plate, and the Y-axis transmission wheel is arranged on the third transmission through hole, so that the whole body is more compact and attractive.
19. The Y-axis sliding block comprises a third sliding block, a fourth sliding block and a camera bracket plate, the Y-axis guide rail comprises a third guide rail and a fourth guide rail, and the third sliding block and the fourth sliding block are respectively in sliding connection with the third guide rail and the fourth guide rail, so that the Y-axis sliding block slides more stably on the Y-axis guide rail.
20. The third sliding block and the fourth sliding block respectively adopt box-type bearings, and the box-type bearings are longer than common bearings, so that the moving rigidity of the Y-axis sliding block is more stable, and the structural rigidity is better.
21. And the fifth box-type bearing, the sixth box-type bearing, the seventh box-type bearing and the eighth box-type bearing are respectively in sliding connection with the third guide rail and the fourth guide rail through a fifth plastic bearing inner sleeve, a sixth plastic bearing inner sleeve, a seventh plastic bearing inner sleeve and an eighth plastic bearing inner sleeve, so that the moving stability of the Y-axis sliding block is improved.
22. The slide scanning mirror of making a video recording includes the microscope tube, Z axle drive mechanism, main camera, the fixed bolster, the bow-shaped frame, the condensing lens, the light source of making a video recording, the microscope tube utilizes Z axle drive mechanism to realize reciprocating focusing, operating personnel's intensity of labour has been reduced, the bow-shaped frame is a dysmorphism piece, the main function is together fixed with condensing lens and light source of making a video recording and whole removal assembly, because during the scanning of making a video recording, condensing lens, the light source of making a video recording need keep on an axis with the microscope tube, this bow-shaped frame design has solved above-mentioned problem, when the slide scanning mirror of making a video recording is Y axle movable scanning simultaneously, bow-shaped position, can effectually avoid with X axle drive mechanism's interference problem.
23. The Z-axis transmission mechanism comprises a Z-axis driving motor, a Z-axis precise moving platform and a screw rod, and the Z-axis driving motor drives the Z-axis precise moving platform to move up and down in the Z-axis direction through the screw rod, so that up-and-down movement focusing of the microscope tube is realized.
24. The lead screw passes through the shaft coupling and is connected with Z axle driving motor, makes the drive connection of Z axle driving motor and lead screw more firm.
25. The microscope tube is arranged on the Z-axis precision moving platform through the tube fixing seat, so that the installation and connection of the microscope tube and the Z-axis precision moving platform are more stable.
26. The Z-axis transmission mechanism also comprises a Z-axis position feedback device which can feed the current real-time position of the microscope cylinder back to the control system.
27. A lens cone through hole and a Z-axis driving through hole are formed in the Y-axis sliding block, the microscope lens cone vertically penetrates through the lens cone through hole to be installed on the lens cone fixing seat, and the shaft coupling penetrates through the Z-axis driving through hole from the lower portion of the Y-axis sliding block to be connected with the Z-axis driving motor, so that the structure is more compact and attractive.
28. The bow-shaped frame comprises a bow-shaped support, a collecting lens fixing plate and an L-shaped bracket, wherein the collecting lens and the camera light source are respectively arranged on the collecting lens fixing plate and the L-shaped bracket, and the collecting lens, the camera light source and the microscope lens barrel are ensured to be on the same axis.
29. The camera shooting light source comprises an LED lamp and a heat dissipation plate, the cost is saved by adopting the LED lamp as the camera shooting light source, the heat dissipation plate provides heat dissipation for the LED lamp, and the service life of the LED lamp is prolonged.
30. The pathological section scanning image analysis system further comprises an auxiliary positioning mechanism, and the auxiliary positioning mechanism is used for positioning the slide loading mechanism to acquire the approximate area of the slide.
31 the luminescent plate passes through the auxiliary light base to be installed on auxiliary light bottom plate crossbeam, and the light outgoing direction of luminescent plate is up, makes things convenient for the auxiliary camera to shoot the content that slide loaded the mechanism.
32. The auxiliary positioning mechanism also comprises an illuminating light source, so that the auxiliary camera can shoot more clear contents on the slide loading mechanism.
33. The luminescent plate is the LED luminescent plate, and light source is the LED light, and the cost is practiced thrift to the LED light source of hi-lite, improves and shoots the definition.
34. The slide loading mechanism comprises a slide rack and a placing support, the slide rack is used for bearing slides, the slide rack is arranged on a slide supporting plate through the placing support, and the placing support is used for positioning and fixing the slide rack.
35. The two sides of the placing support are respectively provided with a first positioning notch and a second positioning notch, so that the placing support can more accurately position the slide frame.
36. The placing support comprises a placing bottom frame, an upper pressing seat and an upper pressing seat cover, wherein the upper pressing seat cover is arranged on the placing bottom frame, a card inserting groove cavity is formed, and the slide frame is connected with the card inserting groove cavity in a matched mode, so that the slide frame is convenient to replace and reset.
37. Be equipped with a plurality of location buckles on first recess, the second recess respectively, make the location that slide frame injected the card slot intracavity more accurate, firm, make slide frame change reset more accurate.
38. The slide rack comprises a handle and a slide bracket, and the handle is arranged to facilitate the slide rack to be pulled out of and inserted into the slot cavity.
39. The slide bracket comprises a slide frame, a plurality of slide separation beams, the slide frame is separated by the slide separation beams to form a plurality of slide placing positions, the slide placing positions are used for bearing slides, the slide bracket is simple in structure, durable, easy to maintain and clean, the operation of placing the slides is simple, and the efficiency is improved. .
40. The slide collet includes first slide collet, second slide collet, with the both ends of first slide collet, second slide collet bearing slide respectively, makes placing of slide more steady.
41. The second slide collet with have the clearance between the second slide holds in the palm to form location strip hole, the setting in location strip hole makes things convenient for the slide frame to be connected with the location of placing the support.
42. Preferably, the number of slide placing positions is five, so that the placed slides can be identified and managed conveniently.
Drawings
Fig. 1 is a first perspective view of a pathological section scanning image analysis system according to an embodiment of the present invention.
Fig. 2 is a first side view of a pathological section scanning image analysis system according to an embodiment of the present invention.
Fig. 3 is a second side view of the pathological section scanning image analysis system according to the embodiment of the present invention.
FIG. 4 is a top view of a pathological section scanning image analysis system according to an embodiment of the present invention.
Fig. 5 is a first perspective view of a base of a pathological section scanning image analysis system according to an embodiment of the present invention.
Fig. 6 is a perspective view of a base of a pathological section scanning image analysis system according to an embodiment of the present invention.
Fig. 7 is a perspective view of an X-axis transmission mechanism of a pathological section scanning image analysis system according to an embodiment of the present invention.
Fig. 8 is a sectional view taken along line a-a of fig. 6.
Fig. 9 is a sectional view taken along line B-B of fig. 6.
FIG. 10 is a top view of the Y-axis transmission mechanism of the pathological section scanning image analysis system according to the embodiment of the present invention.
Fig. 11 is a cross-sectional view of C-C of fig. 6.
Fig. 12 is a cross-sectional view taken along line D-D of fig. 6.
Fig. 13 is a first perspective view of a slide image capture scanning mirror of the pathological section scanning image analysis system according to the embodiment of the invention.
Fig. 14 is a second perspective view of the slide scanning mirror of the pathological section scanning image analysis system according to the embodiment of the present invention.
Fig. 15 is a side view of a slide imaging scan mirror of a pathological section scan image analysis system in accordance with an embodiment of the present invention.
Fig. 16 is a partially enlarged view of the Z-axis transmission mechanism of the slide imaging scanning mirror of the pathological section scanning image analysis system according to the embodiment of the invention.
Fig. 17 is a perspective view of a slide loading mechanism of a pathological section scanning image analysis system according to an embodiment of the present invention.
Fig. 18 is a first perspective view of a placement frame of a slide loading mechanism of a pathological section scanning image analysis system according to an embodiment of the present invention.
FIG. 19 is a second perspective view of a second placement frame of a slide loading mechanism of the pathological section scanning image analysis system according to the embodiment of the present invention.
FIG. 20 is a first schematic perspective view of a slide rack of a slide loading mechanism of a pathological section scanning image analysis system according to an embodiment of the present invention.
FIG. 21 is a second perspective view of a slide rack of the slide loading mechanism of the pathological section scanning image analysis system according to the embodiment of the present invention.
Description of reference numerals:
10. a base, 11, an X-axis transmission mechanism, 111, an X-axis guide rail, 1111, a first guide rail, 1112, a second guide rail, 112, an X-axis slide block, 1121, a first slider, 1121a, a first box bearing, 1121b, a second box bearing, 1121c, a first plastic bearing inner sleeve, 1121d, a second plastic bearing inner sleeve, 1122, a second slider, 1122a, a third box bearing, 1122b, a fourth box bearing, 1122c, a third plastic bearing inner sleeve, 1122d, a fourth plastic bearing inner sleeve, 1123, a slide pallet, 1123a, a pallet light-transmitting hole, 113, an X-axis driving motor, 1131, a first motor, 1132, a second motor, 114, an X-axis transmission wheel, 1141, a first transmission wheel, 1142, a second transmission wheel, 115, an X-axis transmission belt, 1151, a first transmission belt, 1152, a second transmission wheel, 12, a Y-axis transmission mechanism, 121, a Y-axis guide rail, 1211, a third guide rail, 1212, a third guide rail, a fourth guide belt, a third guide belt, a fourth guide belt, a second guide belt, a third guide belt, a fourth guide belt, a third guide belt, a fourth guide belt, a second guide belt, a third guide belt, a fourth guide belt, a third guide belt, a fourth guide belt, a second guide belt, a third guide belt, a fourth guide belt, a second slide block, a third guide belt, a fourth guide belt, a third guide belt, a fourth guide belt, a third guide belt, a fourth guide belt, a third guide belt, a fourth guide belt, a third guide belt, a fourth, A fourth guide rail 122, a Y-axis slide block 1221, a third slide block 1221a, a fifth box bearing 1221b, a sixth box bearing 1221c, a fifth plastic bearing inner sleeve 1221d, a sixth plastic bearing inner sleeve 1222, a fourth slide block 1222a, a seventh box bearing 1222b, an eighth box bearing 1222c, a seventh plastic bearing inner sleeve 1222d, an eighth plastic bearing inner sleeve 1223, a camera carriage plate 1224, a barrel through hole 1225, a Z-axis drive through hole 123, a Y-axis drive motor 124, a Y-axis transmission wheel 125, a Y-axis transmission belt 13, an X-axis mounting bracket 131, an X-bearing front seat plate 1311, a first access notch 132, an X-bearing rear seat plate 133, an X-axis connecting rod 14, a Y-axis mounting bracket 141141, a first door frame 1411, a first column 1412, a second column 1412, a first door frame crossbeam 142, a first door frame 143, a second door frame crossbeam, 144. second rack beam plate, 15, base plate, 20, slide loading mechanism, 21, slide rack, 211, handle, 212, slide carrier, 2121, slide frame, 2121a, first slide holder, 2121b, second slide holder, 2121c, first slide beam, 2121d, second slide beam, 2122, slide separating beam, 213, slide placement position, 214, slide mount, 2141, first slide mount, 2142, second slide mount, 215, positioning strip hole, 22, placement rack, 221, rack clear hole, 222, first positioning notch, 223, second positioning notch, 224, placement chassis, 2241, first groove, 2242, second groove, 2243, third groove, 2244, positioning buckle, 225, placement upper press seat, 226, card slot cavity, 30, scanning microscope, 31, microscope tube, 32, Z-axis drive mechanism, 321, Z-axis drive motor, 322, Z-axis moving stage, 323. the lead screw, 324, the shaft coupling, 325, the lens cone fixing base, 326, Z axle position feedback ware, 33, main camera, 34, the fixed bolster, 35, the bow-shaped frame, 351, the bow-shaped frame, 352, the collecting mirror fixed plate, 353, L shape bracket, 36, the collecting mirror, 37, the light source of making a video recording, 371, the LED lamp, 372, the heating panel, 40, supplementary positioning mechanism, 41, supplementary camera, 42, supplementary fixing base of making a video recording, 43, the luminescent plate, 44, supplementary luminescent bottom plate support, 441, supplementary luminescent base, 442, supplementary luminescent bottom plate crossbeam, 45, illumination light source.
Detailed Description
The following provides a detailed description of embodiments of the invention.
The scanning method of the pathological section scanning image analysis system is applied to a slide shooting scanning mirror 30 of the pathological section scanning image analysis system, wherein the slide shooting scanning mirror 30 comprises a microscope tube 31 and a main camera 33, and the scanning method comprises the following steps:
the magnification of the microscope tube 31 is reduced, so that the depth of field of the microscope tube 31 is increased, and a larger clear imaging area is obtained; the magnification of the microscope tube 31 is six to fourteen times;
the image pickup pixels of the main camera 33 are increased to obtain higher pixel density; the imaging pixel of the main camera 33 is greater than or equal to four hundred and fifty thousand pixels.
The microscope lens barrel 31 comprises an objective lens end and an objective lens end, and the main camera 33 is installed on the objective lens end of the microscope lens barrel 31; the magnification of the microscope tube 31 is eight to twelve times, and the image pickup pixels of the main camera 33 are four hundred and fifty thousand pixels to six million pixels.
Preferably, the magnification of the microscope tube 31 is ten times, and the imaging pixels of the main camera 33 are five million pixels.
The obtaining of the higher pixel density comprises adopting an area array picture acquisition mode, wherein the area array picture acquisition mode comprises the following steps:
firstly, a slice to be scanned is divided into a plurality of bit plane pictures with equal areas;
and synthesizing the bit plane pictures into a digital image.
The horizontal movement of the slide camera scan mirror 30 is performed simultaneously with the vertical in-focus movement.
The system further comprises an auxiliary camera 41 applied to a pathological section scanning image analysis system, and the scanning method comprises the following steps:
after the slide loading mechanism 20 loads the slide on which the pathological sample is placed, moving the slide loading mechanism 20 to a position below the slide shooting scanning mirror 30;
the auxiliary camera 41 takes picture data of the slide, and preliminarily determines a scanning area of the slide to be scanned according to the picture data;
the slide camera scanning mirror 30 scans the pathological sample on the slide in the scanning area.
As shown in fig. 1 to 15, the pathological section scanning image analysis system includes a base 10, a slide loading mechanism 20 and a slide shooting scanning mirror 30 adopting the scanning method, wherein the base 10 includes an X-axis transmission mechanism 11 and a Y-axis transmission mechanism 12, the Y-axis transmission mechanism 12 is located above the X-axis transmission mechanism 11, and the transmission directions of the two are perpendicular to each other; the slide loading mechanism 20 is mounted on the X-axis transmission mechanism 11, and the slide imaging scanning mirror 30 is mounted on the Y-axis transmission mechanism 12. The slide loading mechanism 20 and the slide shooting and scanning mirror 30 are connected with an external computer through data lines, the external computer comprises a controller and shooting output equipment, and the external computer is used for remotely and intelligently focusing and scanning the slide.
The transmission direction of the X-axis transmission mechanism 11 is transmitted along the X-axis direction, the transmission direction of the Y-axis transmission mechanism 12 is transmitted along the Y-axis direction, the X-axis and the Y-axis are mutually vertical, the focusing moving direction of the slide shooting scanning mirror 30 moves along the Z-axis direction, and the Z-axis is vertically vertical to the X-axis and the Y-axis;
the base 10 further comprises an X-axis mounting frame 13, a Y-axis mounting frame 14 and a bottom plate 15, wherein the X-axis mounting frame 13 and the Y-axis mounting frame 14 are respectively mounted on the bottom plate 15, and an X-axis transmission mechanism 11 and a Y-axis transmission mechanism 12 are respectively mounted on the X-axis mounting frame 13 and the Y-axis mounting frame 14; the X-axis mounting frame 13 and the Y-axis mounting frame 14 are made of standard aluminum profiles respectively.
The X-axis mounting frame 13 includes an X-bearing front seat plate 131, an X-bearing rear seat plate 132, and an X-axis connecting rod 133, two ends of the X-axis connecting rod 133 are respectively connected with lower ends of the X-bearing front seat plate 131 and the X-bearing rear seat plate 132, and the X-axis transmission mechanism is mounted on the X-bearing front seat plate 131 and the X-bearing rear seat plate 132.
The X-axis transmission mechanism comprises an X-axis guide rail 111, an X-axis sliding block 112, an X-axis driving motor 113, an X-axis driving wheel 114 and an X-axis transmission belt 115, two ends of the X-axis guide rail 111 are respectively connected with the upper ends of an X-bearing front seat plate 131 and an X-bearing rear seat plate 132, the X-axis sliding block 112 is in sliding connection with the X-axis guide rail 111, the X-axis driving wheel 114 and the X-axis driving motor 113 are respectively installed on the X-bearing front seat plate 131 and the X-bearing rear seat plate 132, the X-axis driving motor 113 is provided with an X-axis driving wheel, two ends of the X-axis transmission belt 115 are connected with two ends of the X-axis sliding block 112 and wound on the X-axis driving wheel 114 and the X-axis driving wheel, and the slide loading mechanism 20 is installed on the X-axis guide rail 112.
The upper ends of the X bearing front seat plate 131 and the X bearing rear seat plate 132 are respectively provided with a first transmission through hole and a second transmission through hole, an X-axis transmission wheel 114 is arranged on the first transmission through hole, an X-axis driving motor 113 is arranged on the outer side of the X bearing rear seat plate 132, one end of the X-axis transmission belt 115 is wound on the X-axis transmission wheel 114, and the other end of the X-axis transmission belt 115 passes through the second transmission through hole and is wound on the X-axis driving wheel.
The X-axis slide block 112 includes a first slide block 1121, a second slide block 1122, a slide tray 1123, the X-axis guide rail 111 includes a first guide rail 1111, a second guide rail 1112, the X-axis drive motor 113 includes a first motor 1131, a second motor 1132, the X-axis drive wheel 114 includes a first drive wheel 1141, a second drive wheel 1142, the X-axis drive belt 115 includes a first drive belt 1151, a second transmission belt 1152, wherein two ends of the first guide rail 1111 and the second guide rail 1112 are respectively connected with two sides of the upper end of the X bearing front seat plate 131 and the upper end of the X bearing rear seat plate 132, the first slider 1121 and the second slider 1122 are respectively connected with the first guide rail 1111 and the second guide rail 1112 in a sliding manner, two sides of the slide support plate 1123 are respectively connected with the first slider 1121 and the second slider 1122, a support light hole 1123a is arranged in the middle of the slide support plate 1123, and the slide loading mechanism 20 is arranged on the slide support plate 1123 and corresponds to the position of the support light hole 1123 a; first drive wheel 1141, second drive wheel 1142 is installed respectively on the upper end both sides of X bearing front seat board 131, first motor 1131, second motor 1132 is installed respectively on the both sides of X bearing rear seat board 132, first motor 1131, second motor 1132 has first action wheel respectively, the second action wheel, the both ends of first drive belt 1151 are connected with the both ends of first slider 1121, and the winding is at first drive wheel 1141, on the first action wheel, the both ends of second drive belt 1152 are connected with the both ends of second slider 1122, and the winding is at second drive wheel 1142, on the second action wheel.
The first slider 1121 includes a first box bearing 1121a and a second box bearing 1121b, the second slider 1122 includes a third box bearing 1122a and a fourth box bearing 1122b, the first box bearing 1121a and the second box bearing 1121b are respectively connected to two ends of one side of the slide supporting plate 1123, and the third box bearing 1122a and the fourth box bearing 1122b are respectively connected to two ends of the other side of the slide supporting plate 1123; the first box bearing 1121a, the second box bearing 1121b, the third box bearing 1122a, and the fourth box bearing 1122b respectively have a first shaft hole, a second shaft hole, a third shaft hole, and a fourth shaft hole, the first guide rail 1111 passes through the first shaft hole and the second shaft hole and is slidably connected to the first box bearing 1121a and the second box bearing 1121b, and the second guide rail 1112 passes through the third shaft hole and the fourth shaft hole and is slidably connected to the third box bearing 1122a and the fourth box bearing 1122 b.
First box bearing 1121a, second box bearing 1121b, third box bearing 1122a, and fourth box bearing 1122b respectively include first plastic bearing inner sleeve 1121c, second plastic bearing inner sleeve 1121d, third plastic bearing inner sleeve 1122c, and fourth plastic bearing inner sleeve 1122d, and first box bearing 1121a, second box bearing 1121b, third box bearing 1122a, and fourth box bearing 1122b are respectively slidably connected to first guide rail 1111 and second guide rail 1112 through first plastic bearing inner sleeve 1121c, second plastic bearing inner sleeve 1121d, third plastic bearing inner sleeve 1122c, and fourth plastic bearing inner sleeve 1122 d.
The middle part of the upper end of the X-bearing front seat plate 131 is provided with a first picking notch 1311, the first picking notch 1311 corresponds to the slide loading mechanism 20, and the first guide 1111 and the second guide 1112 are respectively located on two opposite sides of the first picking notch 1311.
The Y-axis mounting frame 14 includes a first portal frame 141, a second portal frame 142, a first support beam plate 143, a second support beam plate 144, the lower ends of the first portal frame 141 and the second portal frame 142 are respectively connected with the bottom plate 15, the first portal frame 141 and the second portal frame 142 respectively have a first door opening and a second door opening, the first door opening and the second door opening are corresponding, the X-axis mounting frame 13 passes through the first door opening and the second door opening, the two ends of the first support beam plate 143 and the second support beam plate 144 are respectively connected with the upper ends of the first portal frame 141 and the second portal frame 142, and the Y-axis transmission mechanism 12 is mounted on the first support beam plate 143 and the second support beam plate 144.
The Y-axis transmission mechanism 12 comprises a Y-axis guide rail 121, a Y-axis slide block 122, a Y-axis driving motor 123, a Y-axis transmission wheel 124 and a Y-axis transmission belt 125, wherein two ends of the Y-axis guide rail 121 are respectively connected with a first support cross beam plate 143 and a second support cross beam plate 144, the Y-axis slide block 122 is in sliding connection with the Y-axis guide rail 121, the Y-axis transmission wheel 124 and the Y-axis driving motor 123 are respectively arranged on the first support cross beam plate 143 and the second support cross beam plate 144, the Y-axis driving motor 123 is provided with a Y-axis driving wheel, two ends of the Y-axis transmission belt 125 are connected with two ends of the Y-axis slide block 122 and are wound on the Y-axis transmission wheel 124 and the Y-axis driving wheel, and the slide shooting and scanning mirror 30 is arranged on the Y-axis slide block 122.
A third transmission through hole is formed in the first support beam plate 143, the Y-axis transmission wheel 124 is installed on the third transmission through hole, and the Y-axis driving motor 123 is installed on the inner side surface of the second support beam plate 144.
The Y-axis slide block 122 includes a third slide block 1221, a fourth slide block 1222, and a photographing bracket plate 1223, the Y-axis guide rail 121 includes a third guide rail 1211 and a fourth guide rail 1212, both ends of the third guide rail 1211 and the fourth guide rail 1212 are respectively connected to both sides of the first bracket beam plate 143 and the second bracket beam plate 144, the third slide block 1221 and the fourth slide block 1222 are respectively connected to the third guide rail 1211 and the fourth guide rail 1212 in a sliding manner, both sides of the photographing bracket plate 1223 are respectively connected to the third slide block 1221 and the fourth slide block 1222, and the slide photographing scan mirror 30 is mounted on the photographing bracket plate 1223.
The third slider 1221 includes a fifth box bearing 1221a and a sixth box bearing 1221b, the fourth slider 1222 includes a seventh box bearing 1222a and an eighth box bearing 1222b, the fifth box bearing 1221a and the sixth box bearing 1221b are respectively connected to both ends of one side of the imaging tray plate 1223, and the seventh box bearing 1222a and the eighth box bearing 1222b are respectively connected to both ends of the other side of the imaging tray plate 1223; the fifth box bearing 1221a, the sixth box bearing 1221b, the seventh box bearing 1222a, and the eighth box bearing 1222b respectively have a fifth shaft hole, a sixth shaft hole, a seventh shaft hole, and an eighth shaft hole, the third guide 1211 passes through the fifth shaft hole and the sixth shaft hole and is slidably connected to the fifth box bearing 1221a and the sixth box bearing 1221b, and the fourth guide 1212 passes through the seventh shaft hole and the eighth shaft hole and is slidably connected to the seventh box bearing 1222a and the eighth box bearing 1222 b.
The fifth box bearing 1221a, the sixth box bearing 1221b, the seventh box bearing 1222a, and the eighth box bearing 1222b respectively include a fifth plastic bearing inner sleeve 1221c, a sixth plastic bearing inner sleeve 1221d, a seventh plastic bearing inner sleeve 1222c, and an eighth plastic bearing inner sleeve 1222d, and the fifth box bearing 1221a, the sixth box bearing 1221b, the seventh box bearing 1222a, and the eighth box bearing 1222b are slidably connected to the third guide rail 1222 and the fourth guide rail 1212 through the fifth plastic bearing inner sleeve 1221c, the sixth plastic bearing inner sleeve 1221d, the seventh plastic bearing inner sleeve 1222c, and the eighth plastic bearing inner sleeve 1222d, respectively.
The slide shooting scanning mirror 30 comprises a microscope tube 31, a Z-axis transmission mechanism 32, a main camera 33, a fixed support 34, an arc-shaped frame 35, a condenser 36 and a shooting light source 37, wherein the Z-axis transmission mechanism 32 and the arc-shaped frame 35 are installed on a Y-axis sliding block 122 through the fixed support 34, the microscope tube 31 is installed on the Z-axis transmission mechanism 32, the microscope tube 31 comprises an objective end and an objective end, and the main camera 33 is installed on the eyepiece end of the microscope tube 31; the condenser lens 36 and the imaging light source 37 are mounted on the arcuate frame 35 so as to correspond to the microscope tube 31, the condenser lens 36 is positioned above the imaging light source 37, an imaging gap is provided between the microscope tube 31 and the condenser lens 36, and the slide loading mechanism 20 passes through the imaging gap.
The Z-axis transmission mechanism 32 comprises a Z-axis driving motor 321, a Z-axis precise moving platform 322, a screw rod 323, a coupler 324, a lens cone fixing seat 325 and a Z-axis position feedback device 326, wherein the Z-axis driving motor 321 is installed on the fixed support 34, the Z-axis precise moving platform 322 is vertically connected with the fixed support 34 in a sliding manner, the Z-axis driving motor 321 is connected with the Z-axis precise moving platform 322 in a driving manner through the screw rod 323, and the microscope lens cone 31 is installed on the Z-axis precise moving platform 322; the screw rod 323 is connected with a Z-axis driving motor 321 through a coupler 324; the Z-axis transmission mechanism 32 also comprises a microscope lens barrel 31 which is arranged on a Z-axis precision moving platform 322 through a lens barrel fixing seat 325; a Z-axis position feedback device 326 is mounted on the side of the Z-axis precision motion stage 322.
A lens barrel through hole 1224 and a Z-axis driving through hole 1225 are arranged on the Y-axis sliding block 122, the microscope lens barrel 31 vertically penetrates through the lens barrel through hole 1224 and is mounted on the lens barrel fixing seat 325, the Z-axis driving motor 321 is positioned above the Y-axis sliding block 122, and the coupling 324 penetrates through the Z-axis driving through hole 1225 from the lower part of the Y-axis sliding block 122 and is connected with the Z-axis driving motor 321.
The bow 35 comprises a bow support 351, a collecting mirror fixing plate 352 and an L-shaped bracket 353, one end of the bow support 351 is connected with the fixing support 34, the other end of the bow support 351 is connected with the collecting mirror fixing plate 352 and the L-shaped bracket 353, the collecting mirror fixing plate 352 is positioned above the L-shaped bracket 353, and the collecting mirror 36 and the image pickup light source 37 are respectively installed on the collecting mirror fixing plate 352 and the L-shaped bracket.
The image pickup light source 37 includes an LED lamp 371 and a heat dissipating plate 372, the LED lamp 371 and the heat dissipating plate 372 are respectively mounted on the L-shaped bracket, and the LED lamp 371 is located above the heat dissipating plate 372.
The pathological section scanning image analysis system further comprises an auxiliary positioning mechanism 40, wherein the auxiliary positioning mechanism 40 comprises an auxiliary camera 41, an auxiliary camera fixing seat 42, a light-emitting plate 43 and an auxiliary light-emitting bottom plate support 44, the first portal frame 141 comprises a first upright 1411, a second upright 1412 and a first portal top cross beam 1413, two ends of the first portal top cross beam 1413 are respectively connected with the upper ends of the first upright 1411 and the second upright 1412, the light-emitting plate 43 is arranged on the first upright 1411 and the second upright 1412 through the auxiliary light-emitting bottom plate support 44, and the light-emitting plate 43 is positioned below the X-axis transmission mechanism 11; the auxiliary camera 41 is mounted on the first door top beam 1413 through the auxiliary camera fixing base 42, and the imaging direction of the auxiliary camera 41 faces the light emitting plate 43.
The auxiliary light-emitting base plate support 44 includes an auxiliary light-emitting base 441 and an auxiliary light-emitting base plate beam 442, both ends of the auxiliary light-emitting base plate beam 442 are respectively connected to the first pillar 1411 and the second pillar 1412, the light-emitting plate 43 is mounted on the auxiliary light-emitting base plate beam 442 through the auxiliary light-emitting base 441, and the light-emitting direction of the light-emitting plate 43 faces upward.
The auxiliary positioning mechanism 40 further comprises an illumination source 45, the illumination source 45 being mounted on the first door top cross-member 1413; the light-emitting panel 43 is an LED light-emitting panel 43, and the illumination light source 45 is an LED illumination lamp.
The slide loading mechanism 20 includes a rack 22 and the slide rack 21, the slide rack 21 is mounted on the slide support plate 1123 through the rack 22, the rack light hole 221 is provided in the middle of the rack 22, and the slide rack 21 corresponds to the rack light hole 221 and the rack light hole 1123 a.
The two sides of the placing bracket 22 are respectively provided with a first positioning notch 222 and a second positioning notch 223, and the first positioning notch 222 and the second positioning notch 223 are respectively connected with the first sliding block 1121 and the second sliding block 1122 in a matching manner.
The placing support 22 comprises a placing bottom frame 224 and a placing upper pressing seat 225, the placing bottom frame 224 comprises a first side frame strip, a second side frame strip, a first end frame strip and a first end plate, the two ends of the first end frame strip are respectively connected with one end of the first side frame strip and one end of the second side frame strip, the two ends of the first end plate are respectively connected with the other end of the first side frame strip and the other end of the second side frame strip, the first side frame strip, the second side frame strip, the first end frame strip and the first end plate enclose a bracket light hole 1123a, one side of the first side frame strip, the second side frame strip and the first end frame strip facing the bracket light hole 1123a are respectively provided with a first groove 2241, a second groove 2242 and a third groove 2243, the first groove 2241, the second groove 2242 and the third groove 2243 are communicated with the top plate surface of the first end plate, the upper pressing seat 225 is placed to cover the placing bottom frame 224, and forms a card-slot cavity 226, with which the slide frame 21 is fittingly connected.
A plurality of positioning buckles 2244 are arranged on the first groove 2241 and the second groove 2242 respectively, and the slide rack 21 is connected with the card slot cavity 226 through the positioning buckles 2244 in a buckling mode.
The slide rack 21 includes a handle 211 and a slide holder 212, the handle 211 being connected to an outer side surface of one end of the slide holder 212. The slide carrier 212 includes a slide frame 2121, a plurality of slide separation beams 2122 that separate the slide frame 2121 into a plurality of slide placement locations 213, and a slide mount 214 is provided at the bottom end of the slide placement locations 213.
The slide bottom support 214 comprises a first slide bottom support 2141 and a second slide bottom support 2142, the first slide bottom support 2141 and the second slide bottom support 2142 are respectively positioned at two ends of the slide placing position 213, and the first slide bottom support 2141 and the second slide bottom support 2142 are used for supporting two ends of a slide;
the spring buckle 2123 is positioned in the middle of one end of the slide placing position 213, the number of the first slide supports 2141 is two, the two first slide supports 2141 are respectively positioned at two sides of the spring buckle 2123, and the two first slide supports 2141 are respectively connected with the slide frame 2121 or the slide separating beam 2122;
the slide frame 2121 comprises a first slide holder 2121a, a second slide holder 2121b, a first slide beam 2121c and a second slide beam 2121d, wherein two ends of the first slide holder 2121a and the second slide holder 2121b are respectively connected with two ends of the first slide beam 2121c and the second slide beam 2121d, and two ends of a plurality of slide separating beams 2122 are respectively connected with the first slide holder 2121a and the second slide holder 2121b and are positioned between the first slide beam 2121c and the second slide beam 2121 d; the second slide mount 2142 is spaced from the second slide holder 2121b and defines a positioning strip aperture 215, the positioning strip aperture 215 mating with the positioning catch 2244.
The number of the first slide supports 2141 is two, and the two first slide supports 2141 are respectively located on both sides of one end of the slide placing position 213.
The two ends of each second slide mount 2142 are connected to the first slide beam 2121c, the second slide beam 2121d, and the slide separating beam 2122 on the two sides of each slide placing location 213, respectively; preferably, there are five slide placement locations 213, five corresponding spring clips 2123, second slide mounts 2142, and ten first slide mounts 2141;
the above-described embodiment has the following advantages:
1. the scanning method of the pathological section scanning image analysis system is characterized in that the magnification times of the microscope tube 31 are reduced, so that the depth of field of the microscope tube 31 is increased, the longer the depth of field is, the larger the corresponding clear imaging interval is, the larger the tolerance rate is, the focusing difficulty can be reduced, the focusing is rapid, and the requirement on the moving precision of a mechanical structure moving part is relatively low; the shooting pixels of the main camera 33 are improved, so that higher pixel density can be obtained on the same target surface size, and the high-optical-resolution objective lens is matched, so that the image density and the definition degree which can be achieved only by the microscope tube 31 with the reduced magnification factor can be achieved by the microscope tube with the original magnification factor.
2. The magnification of the microscope tube 31 is eight times to twelve times, the image pickup pixels of the main camera 33 are four hundred and fifty thousand pixels to six million pixels, preferably, the magnification of the microscope tube 31 is ten times, the image pick-up pixel of the main camera 33 is five million pixels, the microscope tube 31 commonly used by the slide scanning system in the market is an objective lens with twenty times or forty times, the depth of field of the objective lens with twenty times magnification is generally between 1 micron and 1.4 microns according to the brand, the invention adopts ten times of objective lens, the depth of field of the lens is 3.6 microns, the depth of field of the ten-fold objective lens is three times of that of the twenty-fold objective lens, the depth of field of the ten-fold objective lens is larger than that of the twenty-fold objective lens, therefore, the invention can obtain more focusing contents under fewer layers during scanning, has lower requirements on focusing precision, and has the advantage of lower cost due to the use of the lens with relatively small light transmission amount; the invention adopts the main camera with the shooting pixel density of five million pixels to replace a common camera with two million pixels in the market, obtains higher pixel density on the same target surface size, and is matched with the objective lens with high optical resolution, thereby achieving the pixel density and the definition degree which can be achieved by the original twenty objective lens by using ten times of objective lens.
3. The scanning method adopts an area array picture acquisition mode, and the acquisition mode of a relative line matrix is clearer in the aspect of definition.
4. The horizontal movement and the vertical focusing movement of the slide shooting scanning mirror 30 of the scanning method are simultaneously carried out, so that the focusing movement time is further reduced.
5. The pathological section scanning image analysis system comprises a base 10, a slide loading mechanism 20 and a slide shooting scanning mirror 30, wherein the base 10 comprises an X-axis transmission mechanism 11 and a Y-axis transmission mechanism 12, the slide shooting scanning mirror 30 is vertically focused and horizontally moved along a Y axis, the focusing and the horizontal movement can be simultaneously carried out, the Y-axis movement of the slide shooting scanning mirror 30 is positioned above the X-axis movement of the slide loading mechanism 20 and is effectively separated, so that the structural layout of the whole equipment is more compact, and the base 10 can reduce the occupied space in the moving direction of the Y axis;
this pathological section scanning image analysis system makes slide scanning mirror 30 make a video recording have realized that the Z axle reciprocates to focus and the horizontal migration of Y axle goes on simultaneously, has reduced the removal time, and the effectual X axle that loads with the slide of slide scanning mirror 30 that makes a video recording removes the separation, and mutual noninterference misplaces from top to bottom, and slide frame 21 removes and is difficult to the shake, scans highly stable, improves the scanning definition.
6. The focusing moving direction of the slide shooting scanning mirror 30 is vertical to the X-axis transmission mechanism 11 and the Y-axis transmission mechanism 12, so that the focusing moving direction of the slide shooting scanning mirror 30 is vertical to the slide loading mechanism 20, and the shooting definition is improved.
7. The base 10 further comprises an X-axis mounting frame 13, a Y-axis mounting frame 14 and a bottom plate 15, the X-axis transmission mechanism 11 and the Y-axis transmission mechanism 12 are respectively mounted on the X-axis mounting frame 13 and the Y-axis mounting frame 14, X-axis transmission and Y-axis transmission are effectively separated, up-down dislocation is not interfered with each other, and the structural layout of the equipment is more compact.
8. The X-axis mounting frame 13 and the Y-axis mounting frame 14 are made of standard aluminum profiles, so that the material cost is reduced, the assembly precision requirement is reduced, product modularization is realized, and the production efficiency is improved.
9. The X-axis mounting frame 13 comprises an X-axis bearing front seat plate 131, an X-axis bearing rear seat plate 132 and an X-axis connecting rod 133, and the X-axis transmission mechanism is mounted on the X-axis bearing front seat plate 131 and the X-axis bearing rear seat plate 132 and is convenient to mount.
10. The X-axis transmission mechanism comprises an X-axis guide rail 111, an X-axis sliding block 112, an X-axis driving motor 113, an X-axis driving wheel 114 and an X-axis transmission belt 115, and when the X-axis slide loading mechanism is used, the X-axis sliding block 112 slides back and forth along the X-axis guide rail 111 by starting the positive and negative rotation of the X-axis driving motor 113, so that the slide loading mechanism 20 is driven to move along the X-axis direction.
11. The upper ends of the X bearing front seat plate 131 and the X bearing rear seat plate 132 are respectively provided with a first transmission through hole and a second transmission through hole, an X-axis transmission wheel 114 is installed on the first transmission through hole, one end of an X-axis transmission belt 115 is wound on the X-axis transmission wheel 114, the other end of the X-axis transmission belt 115 passes through the second transmission through hole to be wound on an X-axis driving wheel, the space is saved, an X-axis driving motor 113 is installed on the outer side of the X-axis installation frame 13, and the influence on space operation in the X-axis installation frame 13 is avoided.
12. The X-axis slide block 112 includes a first slide block 1121, a second slide block 1122, and a slide glass support plate 1123, the X-axis guide rail 111 includes a first guide rail 1111 and a second guide rail 1112, and the first slide block 1121 and the second slide block 1122 are slidably connected to the first guide rail 1111 and the second guide rail 1112, respectively, so that the sliding of the X-axis slide block 112 on the X-axis guide rail 111 is more stable.
13. The first slider 1121 and the second slider 1122 are respectively formed by box bearings, and the box bearings are longer than ordinary bearings, so that the X-axis slider 112 has more stable movement rigidity and better structural rigidity.
14. First box bearing 1121a, second box bearing 1121b, third box bearing 1122a, and fourth box bearing 1122b are slidably connected to first guide rail 1111 and second guide rail 1112 through first plastic bearing inner sleeve 1121c, second plastic bearing inner sleeve 1121d, third plastic bearing inner sleeve 1122c, and fourth plastic bearing inner sleeve 1122d, respectively, so that the stability of movement of X-axis slide block 112 is improved.
15. The middle part of the upper end of the X-bearing front seat plate 131 is provided with a first taking notch 1311, which is convenient for the slide loading mechanism 20 to take.
16. The Y-axis mounting rack 14 comprises a first portal frame 141, a second portal frame 142, a first support cross beam plate 143 and a second support cross beam plate 144, the X-axis mounting rack 13 penetrates through the first door hole and the second door hole, so that the Y-axis mounting rack 14 and the X-axis mounting rack 13 are not interfered with each other, the upper part and the lower part of the Y-axis mounting rack are staggered, the structure is more compact, the stability requirement on transportation is reduced, and the maintenance cost is reduced.
17. The Y-axis transmission mechanism 12 includes a Y-axis guide rail 121, a Y-axis slide block 122, a Y-axis driving motor 123, a Y-axis transmission wheel 124, and a Y-axis transmission belt 125, and when in use, the Y-axis slide block 122 slides back and forth along the Y-axis guide rail 121 by starting the forward and reverse rotation of the Y-axis driving motor 123, so as to drive the slide shooting scanning mirror 30 to move along the Y-axis direction.
18. The first support beam plate 143 is provided with a third transmission through hole, and the Y-axis transmission wheel 124 is arranged on the third transmission through hole, so that the whole body is more compact and beautiful.
19. The Y-axis slide block 122 includes a third slider 1221, a fourth slider 1222, and an imaging carriage plate 1223, the Y-axis guide rail 121 includes a third guide rail 1211 and a fourth guide rail 1212, and the third slider 1221 and the fourth slider 1222 are slidably connected to the third guide rail 1211 and the fourth guide rail 1212, respectively, so that the Y-axis slide block 122 can slide on the Y-axis guide rail 121 more stably.
20. The third slider 1221 and the fourth slider 1222 are respectively formed by box bearings, and the box bearings are longer than the conventional bearings, so that the Y-axis slider 122 has more stable movement rigidity and better structural rigidity.
21. The fifth, sixth, seventh and eighth box bearings 1221a, 1221b, 1222a and 1222b are slidably connected to the third and fourth guide rails 1211 and 1212 through fifth, sixth, seventh and eighth plastic bearing inner sleeves 1221c, 1221d, 1222c and 1222d, respectively, thereby improving the stability of the movement of the Y-axis slide block 122.
22. The slide shooting and scanning mirror 30 comprises a microscope tube 31, a Z-axis transmission mechanism 32, a main camera 33, a fixed support 34, an arc-shaped frame 35, a condenser 36 and a shooting light source 37, wherein the microscope tube 31 realizes up-and-down movement focusing by the Z-axis transmission mechanism 32, the labor intensity of operators is reduced, the arc-shaped frame 35 is a special-shaped part and mainly used for fixing the condenser 36 and the shooting light source 37 with the whole moving assembly together, and the condenser 36 and the shooting light source 37 need to be kept on the same axis with the microscope tube 31 during shooting and scanning.
23. The Z-axis transmission mechanism 32 includes a Z-axis driving motor 321, a Z-axis precision moving stage 322, and a lead screw 323, wherein the Z-axis driving motor 321 drives the Z-axis precision moving stage 322 to move up and down in the Z-axis direction through the lead screw 323, thereby realizing up-and-down focusing of the microscope tube 31.
24. The screw rod 323 is connected with the Z-axis driving motor 321 through the coupler 324, so that the driving connection between the Z-axis driving motor 321 and the screw rod 323 is more stable.
25. Microscope tube 31 is mounted on Z-axis precision moving stage 322 through tube mount 325, making the mounting connection of microscope tube 31 and Z-axis precision moving stage 322 more stable.
26. Z-axis actuator 32 further includes a Z-axis position feedback unit 326, and Z-axis position feedback unit 326 can feed the current real-time position of microscope tube 31 back to the control system.
27. A lens barrel through hole 1224 and a Z-axis driving through hole 1225 are arranged on the Y-axis sliding block 122, the microscope lens barrel 31 vertically penetrates through the lens barrel through hole 1224 and is mounted on the lens barrel fixing seat 325, and the coupling 324 penetrates through the Z-axis driving through hole 1225 from the lower part of the Y-axis sliding block 122 and is connected with the Z-axis driving motor 321, so that the structure is more compact and attractive.
28. The bow-shaped frame 35 comprises a bow-shaped support 351, a collecting mirror fixing plate 352 and an L-shaped bracket 353, wherein the collecting mirror 36 and the image pickup light source 37 are respectively arranged on the collecting mirror fixing plate 352 and the L-shaped bracket, and the collecting mirror 36, the image pickup light source 37 and the microscope lens barrel 31 are ensured to be on the same axis.
29. The camera light source 37 comprises an LED lamp 371 and a heat dissipation plate 372, the cost is saved by adopting the LED lamp 371 for the camera light source 37, the heat dissipation plate 372 dissipates heat for the LED lamp 371, and the service life of the LED lamp 371 is prolonged.
30. The pathological section scanning image analysis system further comprises an auxiliary positioning mechanism 40, wherein the auxiliary positioning mechanism 40 is used for positioning the slide loading mechanism 20 to obtain the approximate area of the slide.
31. The light-emitting plate 43 is mounted on the auxiliary light-emitting base plate beam 442 through the auxiliary light-emitting base 441, and the light-emitting direction of the light-emitting plate 43 is upward, so that the auxiliary camera 41 can conveniently shoot the content of the slide loading mechanism 20.
32. The auxiliary positioning mechanism 40 also includes an illumination source 45 to allow the auxiliary camera 41 to capture a clearer image of the contents of the slide loading mechanism 20.
33. The light-emitting plate 43 is an LED light-emitting plate 43, and the illuminating light source 45 is an LED illuminating lamp, and is a high-brightness LED light source, so that the cost is saved, and the shooting definition is improved.
34. The slide loading mechanism 20 includes a slide rack 21 and a placing support 22, the slide rack 21 is used for carrying slides, the slide rack 21 is mounted on the slide support plate 1123 through the placing support 22, and the placing support 22 is used for positioning and fixing the slide rack 21.
35. The placing support 22 is provided with a first positioning notch 222 and a second positioning notch 223 on two sides respectively, so that the placing support 22 can accurately position the slide frame 21.
36. Place support 22 including placing chassis 224, place and press seat 225 lid to close and placing chassis 224 on to form card slot cavity 226, slide frame 21 and card slot cavity 226 looks accordant connection make things convenient for slide frame 21 to change and reset.
37. Be equipped with a plurality of location buckles 2244 on first recess 2241, the second recess 2242 respectively, make slide frame 21 inject location more accurate, firm in the card slot cavity 226, make slide frame 21 change reset more accurate.
38. The slide rack 21 includes a handle 211, a slide holder 212, and the handle 211 is configured to facilitate the removal and insertion of the slide rack 21 from the card slot cavity 226.
39. Slide bracket 212 includes slide frame 2121, a plurality of slide partition roof beams 2122, and a plurality of slides partition roof beams 2122 separate slide frame 2121 and form a plurality of slides and place position 213, and slide places position 213 and is used for bearing the slide, this slide bracket 212 simple structure, durable, easy to maintain, wash, place slide easy operation simultaneously, raise the efficiency.
40. The slide mount 214 includes a first slide mount 2141, a second slide mount 2142, and the first slide mount 2141 and the second slide mount 2142 support the two ends of the slide, respectively, so that the slide can be placed more stably.
41. A gap is provided between the second slide mount 2142 and the second slide mount 2121b and a positioning strip aperture 215 is formed, the positioning strip aperture 215 facilitates the positioning connection of the slide holder 21 to the placement frame 22.
42. Preferably, the slide placing positions 213 are five, which facilitates identification and management of placed slides.
The above are merely specific embodiments of the present invention, and the scope of the present invention is not limited thereby; any alterations and modifications without departing from the spirit of the invention are within the scope of the invention.

Claims (40)

1. The scanning method of the pathological section scanning image analysis system is characterized in that the scanning method is applied to a slide shooting scanning mirror of the pathological section scanning image analysis system, and the slide shooting scanning mirror comprises a microscope tube and a main camera; the horizontal movement and the vertical focusing movement of the slide shooting scanning mirror are carried out simultaneously; the microscope lens barrel comprises an eyepiece end and an objective end, and the main camera is arranged on the eyepiece end of the microscope lens barrel; the scanning method comprises the following steps:
the magnification of the microscope tube is reduced, so that the depth of field of the microscope tube is increased, and a larger clear imaging area is obtained; the magnification of the microscope tube is eight times to twelve times;
the shooting pixels of the main camera are improved, and higher pixel density is obtained; the shooting pixels of the main camera are four hundred and fifty thousand pixels to six million pixels;
the obtaining of the higher pixel density comprises adopting an area array picture acquisition mode, wherein the area array picture acquisition mode comprises the following steps:
firstly, a slice to be scanned is divided into a plurality of bit plane pictures with equal areas;
then synthesizing a plurality of the bit plane pictures into a digital image;
the pathological section scanning image analysis system further comprises an auxiliary camera applied to the pathological section scanning image analysis system, and the scanning method comprises the following steps:
after a slide loading mechanism is loaded with a slide with a pathological sample, moving the slide loading mechanism to the position below the slide shooting scanning mirror;
the auxiliary camera shoots the picture data of the slide, and the scanning area of the slide to be scanned is preliminarily determined according to the picture data;
and the slide shooting scanning mirror scans the pathological sample on the slide in the scanning area.
2. The scanning method of the pathological section scanning image analysis system as claimed in claim 1, wherein the magnification of the microscope tube is ten times, and the image pickup pixels of the main camera are five million pixels.
3. The pathological section scanning image analysis system is characterized by comprising a base, a slide loading mechanism arranged on the base and a slide shooting scanning mirror arranged on the base, wherein the slide shooting scanning mirror comprises a microscope tube and a main camera, and the magnification of the microscope tube is eight times to twelve times; the shooting pixels of the main camera are four hundred and fifty thousand pixels to six million pixels;
the microscope lens barrel comprises an eyepiece end and an objective end, and the main camera is arranged on the eyepiece end of the microscope lens barrel;
the auxiliary camera is arranged on the base;
the base comprises an X-axis transmission mechanism and a Y-axis transmission mechanism, the Y-axis transmission mechanism is positioned above the X-axis transmission mechanism, and the transmission directions of the X-axis transmission mechanism and the Y-axis transmission mechanism are vertical to each other; the slide loading mechanism is arranged on the X-axis transmission mechanism, and the slide shooting scanning mirror is arranged on the Y-axis transmission mechanism;
the base further comprises an X-axis mounting rack, a Y-axis mounting rack and a bottom plate, the X-axis mounting rack and the Y-axis mounting rack are respectively mounted on the bottom plate, and the X-axis transmission mechanism and the Y-axis transmission mechanism are respectively mounted on the X-axis mounting rack and the Y-axis mounting rack;
the Y-axis mounting frame comprises a first portal frame, a second portal frame, a first support cross beam plate and a second support cross beam plate, the lower ends of the first portal frame and the second portal frame are respectively connected with the bottom plate, the first portal frame and the second portal frame are respectively provided with a first door opening and a second door opening, the first door opening and the second door opening are corresponding, the X-axis mounting frame penetrates through the first door opening and the second door opening, two ends of the first support cross beam plate and the second support cross beam plate are respectively connected with the upper ends of the first portal frame and the second portal frame, and the Y-axis transmission mechanism is mounted on the first support cross beam plate and the second support cross beam plate;
the Y-axis transmission mechanism comprises a Y-axis guide rail, a Y-axis sliding block, a Y-axis driving motor, a Y-axis transmission wheel and a Y-axis transmission belt;
the slide shooting scanning mirror comprises a microscope tube, a main camera, a Z-axis transmission mechanism, a fixed support, an arched frame, a condensing mirror and a shooting light source, wherein the Z-axis transmission mechanism and the arched frame are arranged on the Y-axis sliding block through the fixed support; the collecting mirror and the image pickup light source are arranged on the bow-shaped frame and correspond to the microscope lens barrel, the collecting mirror is positioned above the image pickup light source, an image pickup gap is formed between the microscope lens barrel and the collecting mirror, and the slide loading mechanism penetrates through the image pickup gap.
4. The pathological section scanning image analysis system of claim 3, wherein the magnification of the microscope tube is ten times, and the imaging pixels of the main camera are five million pixels.
5. The system for analyzing scanned images of pathological sections according to claim 3, wherein the driving direction of the X-axis driving mechanism is along the X-axis direction, the driving direction of the Y-axis driving mechanism is along the Y-axis direction, the X-axis and the Y-axis are perpendicular to each other, the focusing moving direction of the slide image capturing and scanning mirror is along the Z-axis direction, and the Z-axis is perpendicular to the X-axis and the Y-axis.
6. The pathological section scanning image analysis system of claim 3, wherein the X-axis mounting rack and the Y-axis mounting rack are made of standard aluminum profiles.
7. The pathological section scanning image analysis system of claim 3, wherein the X-axis mounting frame comprises an X-bearing front seat plate, an X-bearing rear seat plate and an X-axis connecting rod, two ends of the X-axis connecting rod are respectively connected with the lower ends of the X-bearing front seat plate and the X-bearing rear seat plate, and the X-axis transmission mechanism is mounted on the X-bearing front seat plate and the X-bearing rear seat plate.
8. The pathological section scanning image analysis system of claim 7, wherein the X-axis transmission mechanism comprises an X-axis guide rail, an X-axis sliding block, an X-axis driving motor, an X-axis driving wheel and an X-axis driving belt, two ends of the X-axis guide rail are respectively connected with the upper ends of the X-axis bearing front seat plate and the X-axis bearing rear seat plate, the X-axis sliding block is connected with the X-axis guide rail in a sliding manner, the X-axis driving wheel and the X-axis driving motor are respectively installed on the X-axis bearing front seat plate and the X-axis bearing rear seat plate, the X-axis driving motor is provided with an X-axis driving wheel, two ends of the X-axis driving belt are connected with two ends of the X-axis sliding block and are wound on the X-axis driving wheel and the X-axis driving wheel, and the slide loading mechanism is installed on the X-axis sliding block.
9. The pathological section scanning image analysis system of claim 8, wherein the upper ends of the X-bearing front seat plate and the X-bearing rear seat plate are respectively provided with a first transmission through hole and a second transmission through hole, the X-axis transmission wheel is installed on the first transmission through hole, the X-axis driving motor is installed outside the X-bearing rear seat plate, one end of the X-axis transmission belt is wound on the X-axis transmission wheel, and the other end of the X-axis transmission belt passes through the second transmission through hole and is wound on the X-axis driving wheel.
10. The pathological section scanning image analysis system of claim 8, wherein the X-axis slide block comprises a first slide block, a second slide block, and a slide supporting plate, the X-axis guide rail comprises a first guide rail and a second guide rail, the X-axis driving motor comprises a first motor and a second motor, the X-axis driving wheel comprises a first driving wheel and a second driving wheel, the X-axis driving belt comprises a first driving belt and a second driving belt, two ends of the first guide rail and the second guide rail are respectively connected with two sides of the upper end of the X-bearing front base plate and the upper end of the X-bearing rear base plate, the first slide block and the second slide block are respectively connected with the first guide rail and the second guide rail in a sliding manner, two sides of the slide supporting plate are respectively connected with the first slide block and the second slide block, a bracket light hole is arranged in the middle of the slide supporting plate, the slide loading mechanism is mounted on the slide supporting plate, and the position of the light hole corresponds to that of the bracket; first drive wheel, second drive wheel are installed respectively on the upper end both sides of X bearing front seat board, and first motor, second motor are installed respectively on the both sides of X bearing rear seat board, first motor, second motor have first action wheel, second action wheel respectively, the both ends of first drive belt are connected with the both ends of first slider to the winding is on first drive wheel, first action wheel, the both ends of second drive belt are connected with the both ends of second slider, and the winding is on second drive wheel, second action wheel.
11. The pathological section scanning image analysis system of claim 10, wherein the first slider includes a first box-type bearing and a second box-type bearing, the second slider includes a third box-type bearing and a fourth box-type bearing, the first box-type bearing and the second box-type bearing are respectively connected to two ends of one side of the slide supporting plate, and the third box-type bearing and the fourth box-type bearing are respectively connected to two ends of the other side of the slide supporting plate; the first box-type bearing, the second box-type bearing, the third box-type bearing and the fourth box-type bearing are respectively provided with a first shaft hole, a second shaft hole, a third shaft hole and a fourth shaft hole, the first guide rail penetrates through the first shaft hole and the second shaft hole and is in sliding connection with the first box-type bearing and the second box-type bearing, and the second guide rail penetrates through the third shaft hole and the fourth shaft hole and is in sliding connection with the third box-type bearing and the fourth box-type bearing.
12. The pathological section scanning image analysis system of claim 11, wherein the first, second, third and fourth box bearings respectively comprise a first, second, third and fourth plastic bearing inner sleeves, and the first, second, third and fourth box bearings are slidably connected to the first and second guide rails respectively through the first, second, third and fourth plastic bearing inner sleeves.
13. The pathological section scanning image analysis system of claim 10, wherein the X-bearing front base plate has a first access notch at the middle of the upper end thereof, the first access notch corresponds to the slide loading mechanism, and the first and second guide rails are respectively located on opposite sides of the first access notch.
14. The pathological section scanning image analysis system of claim 3, wherein the two ends of the Y-axis guide rail are connected to the first and second support beam plates, respectively, the Y-axis slide block is connected to the Y-axis guide rail, the Y-axis driving wheel and the Y-axis driving motor are mounted on the first and second support beam plates, respectively, the Y-axis driving motor has a Y-axis driving wheel, the two ends of the Y-axis driving belt are connected to the two ends of the Y-axis slide block and wound on the Y-axis driving wheel and the Y-axis driving wheel, and the slide scanning mirror is mounted on the Y-axis slide block.
15. The pathological section scanning image analysis system of claim 14, wherein the first frame cross beam plate has a third transmission through hole, the Y-axis transmission wheel is installed on the third transmission through hole, and the Y-axis driving motor is installed on the inner side surface of the second frame cross beam plate.
16. The pathological section scanning image analysis system of claim 14, wherein the Y-axis slide block comprises a third slide block, a fourth slide block, and a camera bracket plate, the Y-axis guide rail comprises a third guide rail and a fourth guide rail, both ends of the third guide rail and the fourth guide rail are respectively connected to both sides of the first bracket beam plate and the second bracket beam plate, the third slide block and the fourth slide block are respectively connected to the third guide rail and the fourth guide rail in a sliding manner, both sides of the camera bracket plate are respectively connected to the third slide block and the fourth slide block, and the slide camera scanning mirror is mounted on the camera bracket plate.
17. The pathological section scanning image analysis system of claim 16, wherein the third slider includes a fifth box-type bearing and a sixth box-type bearing, the fourth slider includes a seventh box-type bearing and an eighth box-type bearing, the fifth box-type bearing and the sixth box-type bearing are respectively connected to two ends of one side of the camera bracket plate, and the seventh box-type bearing and the eighth box-type bearing are respectively connected to two ends of the other side of the camera bracket plate; the fifth box-type bearing, the sixth box-type bearing, the seventh box-type bearing and the eighth box-type bearing are respectively provided with a fifth shaft hole, a sixth shaft hole, a seventh shaft hole and an eighth shaft hole, the third guide rail penetrates through the fifth shaft hole and the sixth shaft hole and is in sliding connection with the fifth box-type bearing and the sixth box-type bearing, and the fourth guide rail penetrates through the seventh shaft hole and the eighth shaft hole and is in sliding connection with the seventh box-type bearing and the eighth box-type bearing.
18. The pathological section scanning image analysis system of claim 17, wherein the fifth, sixth, seventh and eighth box bearings respectively comprise a fifth, sixth, seventh and eighth plastic bearing inner sleeves, and the fifth, sixth, seventh and eighth box bearings are slidably connected to the third and fourth guide rails respectively through the fifth, sixth, seventh and eighth plastic bearing inner sleeves.
19. The pathological section scanning image analysis system according to claim 3, wherein the Z-axis transmission mechanism comprises a Z-axis driving motor, a Z-axis precision moving stage and a screw rod, the Z-axis driving motor is mounted on the fixed support, the Z-axis precision moving stage is vertically and slidably connected with the fixed support, the Z-axis driving motor is drivingly connected with the Z-axis precision moving stage through the screw rod, and the microscope tube is mounted on the Z-axis precision moving stage.
20. The pathological section scanning image analysis system of claim 19, wherein the Z-axis transmission mechanism further comprises a coupler, and the lead screw is connected to the Z-axis driving motor through the coupler.
21. The pathological section scanning image analysis system of claim 20, wherein the Z-axis transmission mechanism further comprises a lens barrel fixing seat, and the microscope lens barrel is mounted on the Z-axis precision moving stage through the lens barrel fixing seat.
22. The pathological section scanning image analysis system of claim 21, wherein the Z-axis transmission mechanism further comprises a Z-axis position feedback device mounted on a side of the Z-axis precision motion stage.
23. The pathological section scanning image analysis system of claim 21, wherein the Y-axis slide block has a tube through hole and a Z-axis driving through hole, the microscope tube vertically passes through the tube through hole and is mounted on the tube holder, the Z-axis driving motor is located above the Y-axis slide block, and the coupling passes through the Z-axis driving through hole from below the Y-axis slide block and is connected to the Z-axis driving motor.
24. The pathological section scanning image analysis system of claim 3, wherein the bow-shaped support comprises a bow-shaped support, a collecting mirror fixing plate and an L-shaped bracket, one end of the bow-shaped support is connected with the fixing support, the other end of the bow-shaped support is connected with the collecting mirror fixing plate and the L-shaped bracket, the collecting mirror fixing plate is located above the L-shaped bracket, and the collecting mirror and the image pickup light source are respectively installed on the collecting mirror fixing plate and the L-shaped bracket.
25. The pathological section scanning image analysis system of claim 24, wherein the camera light source comprises an LED lamp and a heat sink, the LED lamp and the heat sink are respectively mounted on the L-shaped bracket, and the LED lamp is located above the heat sink.
26. The pathological section scanning image analysis system of claim 3, further comprising an auxiliary positioning mechanism, wherein the auxiliary positioning mechanism comprises an auxiliary camera, an auxiliary camera fixing seat, a light-emitting plate and an auxiliary light-emitting base plate support, the first gantry comprises a first upright, a second upright and a first door top beam, two ends of the first door top beam are respectively connected with the upper ends of the first upright and the second upright, the light-emitting plate is mounted on the first upright and the second upright through the auxiliary light-emitting base plate support, and the light-emitting plate is located below the X-axis transmission mechanism; the auxiliary camera passes through the auxiliary camera fixing base is installed on the first door top beam, the direction of making a video recording of auxiliary camera is towards the luminescent plate.
27. The pathological section scanning image analysis system of claim 26, wherein the auxiliary light-emitting base support comprises an auxiliary light-emitting base and an auxiliary light-emitting base beam, two ends of the auxiliary light-emitting base beam are respectively connected to the first and second columns, the light-emitting panel is mounted on the auxiliary light-emitting base beam through the auxiliary light-emitting base, and the light-emitting direction of the light-emitting panel is upward.
28. The pathological section scanning image analysis system of claim 26, wherein the auxiliary positioning mechanism further comprises an illumination source mounted on the first door top beam.
29. The pathological section scanning image analysis system of claim 28, wherein the light-emitting panel is an LED light-emitting panel, and the illumination source is an LED illumination lamp.
30. The pathological section scanning image analysis system of any one of claims 10-13, wherein the slide loading mechanism comprises a slide rack and a placing support, the slide rack is mounted on the slide supporting plate through the placing support, a support light hole is formed in the middle of the placing support, and the slide rack corresponds to the support light hole and the bracket light hole.
31. The pathological section scanning image analysis system of claim 30, wherein the placing support has a first positioning notch and a second positioning notch on two sides thereof, and the first positioning notch and the second positioning notch are respectively connected to the first slide block and the second slide block in a matching manner.
32. The pathological section scanning image analysis system of claim 31, wherein the placement frame comprises a placement bottom frame and a placement upper pressing seat, the placement bottom frame comprises a first side frame strip, a second side frame strip, a first end frame strip and a first end plate, two ends of the first end frame strip are respectively connected to one ends of the first side frame strip and the second side frame strip, two ends of the first end plate are respectively connected to the other ends of the first side frame strip and the second side frame strip, the first side frame strip, the second side frame strip, the first end frame strip and the first end plate enclose the bracket light hole, one sides of the first side frame strip, the second side frame strip and the first end frame strip facing the bracket light hole are respectively provided with a first groove, a second groove and a third groove, the first groove, the second groove and the third groove are communicated with the top plate surface of the first end plate, the placement upper pressing seat covers the placement bottom frame, and a card slot cavity is formed, and the slide rack is connected with the card slot cavity in a matching way.
33. The pathological section scanning image analysis system of claim 32, wherein a plurality of positioning fasteners are disposed on the first groove and the second groove, respectively, and the slide holder is connected to the slot cavity by the positioning fasteners.
34. The pathological section scanning image analysis system of claim 33, wherein the slide rack includes a handle and a slide holder, the handle is connected to an outer side of one end of the slide holder.
35. The pathological section scanning image analysis system of claim 34, wherein the slide carrier includes a slide frame, a plurality of slide separation beams that separate the slide frame into a plurality of slide placement locations, the slide placement locations having a slide shoe at a bottom end thereof.
36. The pathological section scanning image analysis system of claim 35, wherein the slide mount includes a first slide mount and a second slide mount, the first slide mount and the second slide mount are respectively located at two ends of the slide placement location, and the first slide mount and the second slide mount are used for supporting two ends of a slide.
37. The pathological section scanning image analysis system of claim 36, wherein the slide frame includes a first slide holder, a second slide holder, a first slide beam, and a second slide beam, both ends of the first slide holder and the second slide holder are respectively connected to both ends of the first slide beam and the second slide beam, both ends of the plurality of slide separation beams are respectively connected to the first slide holder and the second slide holder and are located between the first slide beam and the second slide beam; and a gap is reserved between the second slide bottom support and the second slide support, a positioning strip hole is formed, and the positioning strip hole is matched with the positioning buckle.
38. The pathological section scanning image analysis system of claim 37, wherein there are two first slide supports, one on each side of the slide placement location.
39. The pathological section scanning image analysis system of claim 38, wherein each of the second slide supports has two ends that are connected to the first slide beam, the second slide beam, and the slide separation beam on either side of the slide placement location.
40. The pathological section scanning image analysis system of claim 38, wherein the slide placement locations are five, the corresponding spring clips, the second slide mount are five, and the first slide mount is ten.
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