CN110151125A - A kind of application and method based on nail spontaneous fluorescence intensity as biomarker in the product that preparation occurs for diagnosing - Google Patents
A kind of application and method based on nail spontaneous fluorescence intensity as biomarker in the product that preparation occurs for diagnosing Download PDFInfo
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- CN110151125A CN110151125A CN201810153147.2A CN201810153147A CN110151125A CN 110151125 A CN110151125 A CN 110151125A CN 201810153147 A CN201810153147 A CN 201810153147A CN 110151125 A CN110151125 A CN 110151125A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention provides a kind of applications and method based on nail spontaneous fluorescence intensity as biomarker in the product that preparation occurs for diagnosing, the molecular marked compound is using 400nm to the spontaneous fluorescence intensity of the excitation subject human nails between 750nm, and the wave-length coverage of the autofluorescence is in 420nm between 800nm.Noninvasive, efficient, the simple, economy of the present invention, accuracy are high and reproducible.
Description
Technical field
The present invention relates to a kind of intensity of nail autofluorescence, as the method for judging lung cancer morbidity.Specifically refer to logical
It crosses and compares nail spontaneous fluorescence intensity, judge whether subject suffers from the degree of lung cancer and lung cancer morbidity.
Background technique
It is also the highest malignant tumour of China's morbidity and mortality that lung cancer, which is in world wide, with grade malignancy
High, the features such as progress is fast, weak curative effect.The people of lung cancer presumptive signs is first appeared for one, carrying out a rabat detection can mention
Show apparent agglomerate, the problems such as mediastinum film expansion, atelectasis.CT images are can to provide more disease informations.Bronchoscope
Inspection and CT are often used to extract tumor sample for histopathological analysis judgement.On rabat, it is seen that lung tumors one
As be solitary pulmonary nodule form.However, since many other diseases also have secondary feature, including hamartoma, infectiousness granulation
Tumor etc..So also needing further to diagnose.For lung cancer, conclusive diagnosis is the lump sample for taking suspected tumor, utilizes group
Pathological method is knitted to determine.Clinical practice guideline suggestion periodically checks Lung neoplasm.But CT is due to its radiation hazradial bundle,
It is not recommended that high-frequency or prolonged use.
The lung cancer that clinically can detecte relatively early stage using CT images technology at present, if tumour very little, 5 annual survival rates
It can be more than 80%.But major problem is that, majority of populations do not have the habit of periodic detection lung cancer.CT images are inconvenient simultaneously
It is detected in everybody.And these image methods, and cough are only limitted to for the detection method of lung cancer at present, and expiratory dyspnea, hemoptysis,
The apparent rough judgement such as systemic weight reducing and anorexia.Very inconvenient for patient, time-consuming relatively long and price is costly.
Therefore, the method for judging lung cancer and whether falling ill that is noninvasive, quick, economic, can using with family, Ke Yiyong are invented
Come in physical examination screening go out patients with lung cancer noninvasive, quick, economic method and it is noninvasive, quick, economical, can be made with family
Judge the method for lung cancer for prognosis, there is extremely important social and economic significance and clinical value.
Summary of the invention
The purpose of the present inventor is to break through the problem of the prior art, proposes that one kind can be used for detecting lung cancer and to human body
Undamaged novel detection method and application.
The present inventor detects the nail autofluorescence of subject, and it is aobvious to find that lung cancer patient nail autofluorescence is shown after detection
It writes and is higher than non-lung cancer crowd, and fluorescence intensity is higher, then lung cancer severity is higher.The present invention is in the spontaneous glimmering intensity of nail
Rise the detection method and its application as the new marker for judging lung cancer generation.
On the one hand, the present invention provides a kind of molecular marked compounds to prepare answering in the product for judging lung cancer morbidity
With, wherein the molecular marked compound is strong using the autofluorescence of 400nm to excitation subject's nail between 750nm
Degree, the wave-length coverage of the autofluorescence is in 420nm between 800nm.
In a specific embodiment, when the spontaneous fluorescence intensity value of the excitation subject nail is higher than non-lung
The spontaneous fluorescence intensity value of cancer patient's nail, it is determined that subject suffers from lung cancer.
In a specific embodiment, when the spontaneous fluorescence intensity value of the excitation subject nail is higher,
Lung cancer degree is more serious.
In a specific embodiment, the exciting light ranges preferably from 460nm-640nm, more preferably 460nm-
580nm。
In a specific embodiment, the reception optical range is preferably 480nm-750nm, more preferably 500nm-
620nm。
On the other hand, the invention discloses a kind of methods for detecting lung cancer, wherein the following steps are included:
(1) pass through 400nm to excitation patient's human body surface between 750nm；
(2) 420nm is received to the autofluorescence between 800nm；
(3) intensity of autofluorescence is analyzed, it is determined whether be lung cancer.
In a specific embodiment, when the spontaneous fluorescence intensity value of the excitation subject nail is higher than non-lung
The spontaneous fluorescence intensity value of cancer patient's nail, it is determined that subject suffers from lung cancer.
In a specific embodiment, when the spontaneous fluorescence intensity value of the excitation subject nail is higher,
Lung cancer degree is more serious.
The present invention has done many experiments, and the body surface of many other Disease body surface fluorescence and healthy population has also been made
Fluorescence.It was found that the body surface autofluorescence of the patient of the types disease such as normal person and hypertension does not all rise significantly.
Detailed description of the invention:
Fig. 1 is shown to be excited down using blue light, detects the autofluorescence of subject's nail, the autofluorescence of lung cancer or non-lung cancer
Intensity represents figure.
Specific embodiment
Below will be by specifically describing, the present invention is further illustrated.
Unless otherwise defined, all technical and scientific terms used herein have and the technical field of the invention
Those of ordinary skill be generally understood identical meaning.
As described herein, term " autofluorescence ", " autofluorescence ", " spontaneous fluorescence ", " auto-fluorescence "
It is interchangeable, refer to that tissue, cell, biological substance after by the irradiation of the exciting light of appropriate wavelength, absorb the energy of exciting light
Into excitation state, the phenomenon of light longer than excitation wavelength is launched when exiting excitation state, wherein autofluorescence is than swashing
The longer light of emission wavelength.
As described herein, term " exciting light " is the light for referring to excitation biomolecule and autofluorescence phenomenon occurring, wave
Length should be shorter than autofluorescence.
Described herein, nail fluorescence refers to the fluorescence of nail cut or do not cut.
Embodiment 1: exciting light is the relationship of nail spontaneous fluorescence intensity and lung cancer for blue wave band.
According to the present invention, to lung cancer or non-lung cancer nail, skin autofluorescence is carried out using instrument non-invasively imaged in real time.
Experimental result is shown, compared to the spontaneous fluorescence intensity of the nail of non-lung cancer crowd, the autofluorescence of the corresponding position of patients with lung cancer
Intensity significantly increases.(Fig. 1)
Based on technology of the invention, (including but not limited to) will be applied to: 1. screening lung cancer in crowd's physical examination.2. using
In self screening of the lung cancer of family.3. being used for the diagnosis of situation of all-level hospitals (including community hospital and other situation of all-level hospitals) lung cancer.4.
The situation of all-level hospitals of detection for family and to(for) patients with lung cancer rehabilitation degree.
Those skilled in the art is it should be understood that although for illustrative purposes, this document describes tools of the invention
Body embodiment, but it can be carry out various modifications without departing from the spirit and scope of the present invention.Therefore, of the invention specific
Embodiments and examples should not be considered as limiting the scope of the invention.The present invention is limited only by the appended claims.This Shen
Please in quote all documents be fully incorporated herein by reference.
Claims (12)
1. it is a kind of based on nail spontaneous fluorescence intensity as biomarker preparation for diagnosing occur product in
Using, which is characterized in that the molecular marked compound is to use 400nm to excitation subject's nail between 750nm
Spontaneous fluorescence intensity, the wave-length coverage of the autofluorescence is in 420nm between 800nm.
2. application as described in claim 1, which is characterized in that the exciting light ranges preferably from 460nm-640nm, more preferably
For 460nm-580nm.
3. application as described in claim 1, which is characterized in that the reception optical range is preferably 480nm-750nm, more preferably
For 500nm-620nm.
4. application as described in claim 1, which is characterized in that the nail fluorescence is clip detection or surveys in physical examination.
5. application as described in claim 1, which is characterized in that when subject's nail fluorescence intensity is higher than the non-lung cancer measured
When the average body surface fluorescence of crowd, it is determined that subject suffers from lung cancer.
6. application as described in claim 1, which is characterized in that when subject's nail fluorescence intensity is higher, lung cancer degree is tighter
Weight.
7. it is a kind of based on nail spontaneous fluorescence intensity as biomarker preparation for diagnosing occur product in
Method, wherein the following steps are included:
(1) pass through 400nm to excitation patient's nail between 750nm；
(2) 420nm is received to the autofluorescence between 800nm；
(3) intensity of autofluorescence is analyzed, it is determined whether be lung cancer.
8. the method for claim 7, wherein measured when excitation subject's nail fluorescence intensity level is higher than
When the average body surface autofluorescence of non-lung cancer tester, it is determined that subject suffers from lung cancer.
9. the method for claim 7, wherein the exciting light ranges preferably from 460nm-640nm, more preferably
460nm-580nm。
10. the method for claim 7, wherein the reception optical range is preferably 480nm-750nm, more preferably
500nm-620nm。
11. judging the method for lung cancer based on nail spontaneous fluorescence intensity, it is established that the instrument and equipment that detection lung cancer occurs.
12. judging the application of lung cancer based on nail spontaneous fluorescence intensity, which is characterized in that be that screening detects subject in physical examination
Whether lung cancer is suffered from, or detect whether subject suffers from lung cancer in family and situation of all-level hospitals, or in family and doctor at different levels
The rehabilitation degree of tested patients with lung cancer is detected in institute.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113995377A (en) * | 2021-09-16 | 2022-02-01 | 上海交通大学 | Smoking damage assessment device and method |
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Application publication date: 20190823 |