CN110146499A - A kind of dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide and preparation method thereof - Google Patents
A kind of dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide and preparation method thereof Download PDFInfo
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- CN110146499A CN110146499A CN201910543119.6A CN201910543119A CN110146499A CN 110146499 A CN110146499 A CN 110146499A CN 201910543119 A CN201910543119 A CN 201910543119A CN 110146499 A CN110146499 A CN 110146499A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The present invention relates to biochemistry detection technical field, in particular to the dry plate detection reagent card and preparation method thereof of a kind of quantitative detection concentration of hydrogen peroxide.The dry plate detection reagent card includes three laminations, is followed successively by support layer, color layer and diffusion layer；Reagent in color layer includes hydrophilic colloid, surfactant, chromogen A, chromogen B, chromogen C；Reagent in diffusion layer includes hydrophilic high molecular polymer, surfactant, particulate matter, secondary heme hexapeptide.The dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide of the present invention, can in quantitative detection food hydrogen peroxide residual quantity；Dry plate detection reagent card includes three laminations, is followed successively by support layer, color layer and diffusion layer；Color development system uses double-colored substance system, that is, improves the range of linearity of detection, and reduce the detection lower bound of dry plate detection reagent card；Peroxidase is replaced with secondary heme hexapeptide, detection card is made to be not required to addition confactor, reduce the wide in range not easy in inactivation of cost, condition of storage.
Description
Technical field
The present invention relates to biochemistry detection technical field, in particular to the dry plate of a kind of quantitative detection concentration of hydrogen peroxide detects
Reagent card and preparation method thereof.
Background technique
Hydrogen peroxide (H2O2), also known as hydrogen peroxide is that a kind of have strong oxidizing property, corrosive colourless transparent liquid.Peroxide
Change hydrogen as the important chemical products of one kind be often used as disinfectant, bleaching agent, oxidant, liquid fuel etc. food, medicine,
It is widely applied in the fields such as national defence, textile, papermaking, chemical synthesis and environmental protection.National health and Family Planning Committee exist
Food additives are not allow for residual peroxide using providing in standard GB2760-2014 in food.But some food
Using hydrogen peroxide as cheap oxidant, bleaching agent, excess or illegally use cause hydrogen peroxide in food residual for manufacturing enterprise
Stay it is exceeded, sometimes even can cause to poison by food.It hydrogen peroxide manufacture and can lead to using the nonstandard discharge of enterprise to human body
It causes damages with environment.Therefore, it is of great significance to hydrogen peroxide detection in agriculture, sideline product and environment.
Currently, the analysis method about concentration of hydrogen peroxide depends on the oxidation of hydrogen peroxide, pass through peroxide
The catalysis of compound enzyme (Peroxidase, POD) makes the substance of production for colorimetric, luminous, fluorescence or electrochemical signals detection.
Wherein, the iodimetric titration in conventional titration is that the side of content of hydrogen peroxide in detection bleaching process is most widely used in mill practices
Method, this method is easy to operate, but sensitivity is lower, and disturbing factor is more, and terminal discoloration is unobvious.Chemical method has sensitivity
Height, specificity are good, but the measurement range of linearity is narrow, or detection lower bound is inadequate.It is most of with modern instrumental analysis and liquid reagent
The content of hydrogen peroxide measuring method of cooperation needs expensive equipment, complicated for operation, lacks the popularity of application and is generally applicable in
Property.
POD has high substrate specificity, mild condition and efficient remarkable advantage as biocatalyst.But POD makees
It is unstable for native enzyme, it is easy to denaturation (such as inferior in high temperature, highly acid and alkaline condition) occur, analysis is caused to miss
Difference；It also has that purifying is difficult, is not easy to store and expensive simultaneously.
Dry plate detection reagent card has many advantages, such as easy, quick, flexible and operates without professional.Country's base at present
This is qualitative or semiquantitative determination, and this dry plate detection reagent card is by a kind of lamella such as filter paper, cellulose tablet, porous glue film
Substance whole reagents fixed in advance, two layers and three layers by the processing such as stacking, bonding, punching press combination.It is little to measure the range of linearity.
There are many reagents in analysis test to be pre-mixed, and complicated test fluid needs to pre-process, and which limits dry plate inspections
Test agent card application field.
Summary of the invention
In view of this, the present invention provides a kind of dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide and its preparations
Method.The dry plate detection reagent card color development system uses double-colored substance system, that is, improves the range of linearity of detection, and reduce dry
The detection lower bound of piece detection reagent card；Replace peroxidase with secondary heme hexapeptide, make detection card be not required to addition confactor,
Reduce the wide in range not easy in inactivation of cost, condition of storage.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide, dry plate detection reagent Ka Bao
Containing three laminations, it is followed successively by support layer, color layer and diffusion layer；
Reagent in color layer includes hydrophilic colloid, surfactant, chromogen A, chromogen B, chromogen C；Chromogen A is 4- ammonia
Base antipyrine, chromogen B are 1,7- dihydroxy naphthlene, and chromogen C is selected from phenol, parachlorphenol, P-hydroxybenzoic acid, 3,5- dichloro hydroxyl
One of benzene sulfonic acid sodium salt；
Reagent in diffusion layer includes hydrophilic high molecular polymer, surfactant, particulate matter, secondary heme six
Peptide.
It is with the spetrophotometry that 4-AA, 3,5- dichloro sodium hydroxybenzenesulfonate, 1,7- dihydroxy naphthlene form
Example, testing principle of the present invention are as follows: liquid sample is added dropwise in analoids diffusion layer, is uniformly distributed, while passing through time blood in diffusion layer
Red pigment hexapeptide (DhHP-6) is catalyzed, and the hydrogen peroxide in sample decomposites the free radical of oxygen, is pacified the 4- amino in color layer and is replaced
It is oxidized to red quinoid chemical combination respectively than woods and 3,5- dichloro sodium hydroxybenzenesulfonate, 4-AA and 1,7- dihydroxy naphthlene
Object, under 510nm wavelength, the variation through reflective spectrophotometry measurement reflectivity calculates hydrogen peroxide by standard curve
Concentration.Measurement can be completed in 5 minutes.Sample is added dropwise in diffusion layer, is measured in opposite one side, colored intensity is high, reduces
Be loaded end measurement generate bubble, excess liq, turbidity and caused by interfere.Simultaneously because color layer is hydrophilic colloid, have
Very high colour developing evenness.
General color development system is made of using chromogen A and B, and A is 4-AA, and B is selected from phenol, parachlorphenol, right
One of hydroxybenzoic acid, 3,5- dichloro sodium hydroxybenzenesulfonate.Such color development system detection hydrogen peroxide range of linearity is general
It can only be in 3-100mg/L.Color development system of the present invention uses double-colored substance system, i.e. chromogen is made of A, B and C, and A is that 4- amino peace is replaced
Than woods, B 1,7- dihydroxy naphthlene, C is selected from phenol, parachlorphenol, P-hydroxybenzoic acid, one in 3,5- dichloro sodium hydroxybenzenesulfonate
Kind.The color development system detection hydrogen peroxide range of linearity of the present invention can reach the range of linearity that 0.3-400mg/L improves detection,
The detection lower bound of dry plate detection reagent card is reduced again.
Secondary heme hexapeptide (Deuterohemin-AlaHisThrValGluLys, DhHP-6) is using ferroheme as prothetic group
The analogies with peroxidase activity, structural formula is as follows:
DhHP-6 is a kind of peroxidase peptides analogies containing His, has molecular structure stabilized, and molecular weight is small,
DhHP-6 can be catalyzed H2O2Aoxidize 4-AAP- phenol color development system, the catalysis characteristics of Mimetic enzyme.With not needing to assist
The factor, value be cheap, not easy in inactivation the characteristics of, the cost of hydrogen peroxide assay reagents can be reduced, be conducive to improve the steady of reagent
It is qualitative.
Preferably, chromogen C is 3,5- dichloro sodium hydroxybenzenesulfonate.
Preferably, the reagent dosage in color layer is as follows:
Preferably, the reagent dosage in color layer is as follows:
Preferably, hydrophilic colloid be selected from polyvinyl alcohol, amylopectin, xanthan gum, sodium alginate, gum arabic,
Peach gum, chitosan, cellulose esters, polyvinylpyrrolidone, polyacrylamide, hydroxypropyl methyl cellulose, carboxymethyl cellulose
One or more of sodium, Copolymer of Methyl Vinyl Ether/Maleic Anhydride.
Preferably, hydrophilic colloid be selected from one of polyvinyl alcohol, xanthan gum, chitosan, polyvinylpyrrolidone or
It is several.
Preferably, the reagent dosage in diffusion layer is as follows:
Preferably, the reagent dosage in diffusion layer is as follows:
Preferably, hydrophilic high molecular polymer is selected from polyurethane, polyamide, cellulose acetate, nitrocellulose, gathers
Vinyl alcohol, polyvinylpyrrolidone, polyvinyl butyral, amylopectin, xanthan gum, sodium alginate, gum arabic, peach
One or more of glue, chitosan, polyvinylacetate.
Preferably, hydrophilic high molecular polymer is selected from one of polyurethane, cellulose acetate, nitrocellulose or several
Kind.
Preferably, particulate matter be selected from titanium dioxide, barium sulfate, crystallite colloid, silica, resin bead, bead,
One or more of diatomite, cellulose esters.
Preferably, particulate matter is selected from one or both of titanium dioxide, cellulose esters.
Preferably, surfactant be sorbitanmonolaureate, polyoxyethylene sorbitan monooleate 20,
Polyethylene glycol is to one or more of isooctyl phenyl ether.
Preferably, surfactant is polyoxyethylene sorbitan monooleate 20.
Preferably, support layer is the polymer of polyethylene terephthalate, with a thickness of 50~300 microns.
Preferably, support layer is with a thickness of 100~200 microns.
It is highly preferred that support layer is with a thickness of 150~200 microns.
Preferably, the surface of support layer is by coating bottom layer treatment.
The present invention also provides the preparation methods of the dry plate detection reagent card, include the following steps:
Developing solution reagent is coated on support layer, is dried for the first time；Diffusion liquid reagent is sprayed on support layer, is done again
It is dry, obtain include support layer, color layer and diffusion layer dry plate detection reagent card.
Preferably, dry temperature is 40~60 DEG C for the first time, the dry time is 3~5min for the first time；It dries again
Temperature is 40~50 DEG C, and the dry time is 5~10min again.
The present invention provides dry plate detection reagent cards of a kind of quantitative detection concentration of hydrogen peroxide and preparation method thereof.This is dry
Piece detection reagent card includes three laminations, is followed successively by support layer, color layer and diffusion layer；Reagent in color layer includes hydrophily
Colloid, surfactant, chromogen A, chromogen B, chromogen C；Chromogen A is 4-AA, and chromogen B is 1,7- dihydroxy naphthlene,
Chromogen C is selected from one of phenol, parachlorphenol, P-hydroxybenzoic acid, 3,5- dichloro sodium hydroxybenzenesulfonate；Reagent in diffusion layer
Including hydrophilic high molecular polymer, surfactant, particulate matter, secondary heme hexapeptide.The technical effect that the present invention has
It is as follows:
The dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide of the present invention, can hydrogen peroxide in quantitative detection food
Residual quantity；Dry plate detection reagent card includes three laminations, is followed successively by support layer, color layer and diffusion layer；Color development system is using double
Chromogen system, that is, improve the range of linearity of detection, and reduces the detection lower bound of dry plate detection reagent card；With secondary heme six
Peptide replaces peroxidase, and detection card is made to be not required to addition confactor, reduce the wide in range not easy in inactivation of cost, condition of storage；
Operating process is simple, and pattern detection is directly added dropwise without preparing in reagent；Analoids dry no moisture, and analoids are steady
It is qualitative good；Testing result is reproducible, accuracy is high, can carry out quantitative detection, and the range of linearity is wide；Preparation process is easy, easily
Operation, harmfulness, pollution are small.
Specific embodiment
The invention discloses dry plate detection reagent card of a kind of quantitative detection concentration of hydrogen peroxide and preparation method thereof, abilities
Field technique personnel can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that all similar replaces
Change and change apparent to those skilled in the art, they are considered as being included in the present invention.Side of the invention
Method and application be described by preferred embodiment, related personnel obviously can not depart from the content of present invention, spirit and
To method described herein and application is modified or appropriate changes and combinations in range, carry out implementation and application the technology of the present invention.
Term is explained:
Colourless reduced form 4-AA and phenolic substances is oxidized to red under the free radical oxidation of oxygen
Quinonoid compound can measure under 510nm wavelength.
Monochromatic former: phenolic substances only selects phenol, parachlorphenol, P-hydroxybenzoic acid, 3,5- dichloro sodium hydroxybenzenesulfonate, 1,
One of 7- dihydroxy naphthlene substance.
Double-colored original: a kind of selection 1,7- dihydroxy naphthlene of phenolic substances, another kind selection phenol, parachlorphenol, para hydroxybenzene first
One of acid, 3,5- dichloro sodium hydroxybenzenesulfonate substance.
In a specific embodiment provided by the invention, the dry plate detection reagent card reagent of quantitative detection concentration of hydrogen peroxide
It forms as follows:
The formula of color layer reagent is as follows:
The formula of diffusion layer reagent is as follows:
In another specific embodiment provided by the invention, the dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide is tried
Agent composition is as follows:
The formula of color layer reagent is as follows:
The formula of diffusion layer reagent is as follows:
Examination used in dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide provided by the invention and preparation method thereof
Agent and material are available on the market.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1: the preparation method of hydrogen peroxide dry plate detection reagent card of the present invention
Transparent support layer material uses polyethylene terephthalate material, with a thickness of 0.20mm.Surface is by coating
Bottom layer treatment.
The formula of developing solution reagent is as follows:
The formula of diffusion liquid reagent is as follows:
Color layer is coated on the polyethylene terephthalate support layer by coating bottom layer treatment, is put into drying box
In, 60 DEG C dry 3 minutes.
Diffusion liquid is uniformly sprayed on reagent layer, is put into drying box and dries.15mm is cut to strip cutting machine
The test-paper of × 15mm size, is sealed.
Detection method: 10 μ L test samples are added dropwise in analoids, reaction after five minutes, is surveyed with baffled photometer
It is fixed, the concentration of hydrogen peroxide in sample is calculated according to standard curve.
Comparative example 1: using the preparation method of the hydrogen peroxide dry plate detection reagent card of monochromatic former colour developing
For embodiment 1, except, using monochromatic original, other are constant in developing solution.
The formula of developing solution reagent is as follows:
Comparative example 2: using the preparation method of the hydrogen peroxide dry plate detection reagent card of Catalyzed Synthesis By Peroxidase
For embodiment 1, except, using peroxidase and containing corresponding confactor, other are constant in diffusion liquid.
The formula of diffusion liquid reagent is as follows:
Test example 1: the linear analysis and detection lower bound analysis of 1 the method for the embodiment of the present invention
Test sample: high-strength hydrogen peroxide sample (500mg/L)；
Hydrogen peroxide sample is diluted to 12 different concentration, be followed successively by 0mg/L, 20mg/L, 40mg/L, 50mg/L,
60mg/L、80mg/L、100mg/L、120mg/L、200mg/L、300mg/L、400mg/L、500mg/L。
The dry plate detection reagent card that above-mentioned sample is prepared in embodiment 1 and comparative example 1 using the detection method of embodiment 1
3 detections are carried out, coefficient R value is calculated, testing result is shown in Table 1, table 2.
The dry plate detection reagent card line test result of 1 embodiment 1 of table preparation
The dry plate detection reagent card line test result of 2 comparative example 1 of table preparation
The results show that 1 dry plate detection reagent of comparative example is stuck in the range of 3mg/L-100mg/L, R value is 0.9981, tool
There is the good linearity；And 1 dry plate detection reagent of embodiment is stuck in the range of 0.3mg/L-400mg/L, R value is 0.9988,
With the good linearity；Show that the method for the invention has the higher range of linearity and lower detection lower bound.
Test example 2: the stability analysis of 1 the method for the embodiment of the present invention
Condition of storage: sealing is placed under the conditions of 15-30 DEG C of room temperature.
Round of visits: when room temperature 15-30 DEG C 0, March, September, December, 14 months.
Test sample: hydrogen peroxide sample (50mg/L)；
With the detection method of embodiment 1 in above-mentioned round of visits, detected with dry plate prepared by embodiment 1 and comparative example 2
Reagent card carries out 2 detections to 50mg/L hydrogen peroxide sample, and testing result is shown in Table 3.
3 stability test result of table
The results show that being calculated according to the testing result that 1 detection method of embodiment obtains, 2 dry plate detection reagent of comparative example
Measured value and theoretical value deviation are within 10% in card 0-3 months；And 1 dry plate detection reagent of embodiment is stuck in 0-12 months and surveys
Magnitude and theoretical value deviation are within 10%；Show that the method for the invention has under the conditions of 15-30 DEG C preferably to stablize
Property.
Embodiment 2: the preparation method of hydrogen peroxide dry plate detection reagent card of the present invention
Transparent support layer material uses polyethylene terephthalate material, with a thickness of 0.15mm.Surface is by coating
Bottom layer treatment.
The formula of developing solution reagent is as follows:
The formula of diffusion liquid reagent is as follows:
Color layer is coated on the polyethylene terephthalate support layer by coating bottom layer treatment, is put into drying box
In, 40 DEG C dry 5 minutes.
Diffusion liquid is uniformly sprayed on reagent layer, is put into drying box and dries.10mm is cut to strip cutting machine
The test-paper of × 10mm size, is sealed.
Detection method: 15 μ L test samples are added dropwise in analoids, reaction after five minutes, is surveyed with baffled photometer
It is fixed, the concentration of hydrogen peroxide in sample is calculated according to standard curve.
Comparative example 3: the preparation method of hydrogen peroxide dry plate detection reagent card described in other methods
PES film is immersed in using polyethylene terephthalate material with a thickness of 0.20mm by transparent support layer material
It in A liquid, takes out, strikes off after soaking completely, be put into 30-50 DEG C of drying box, continue to be immersed in B liquid after dry, soak completely
It takes out, strikes off after wet.It is put into 30 DEG C of drying box, is taken out after dry, PES film is pasted on support layer.
The formula of A liquid reagent is as follows:
1,7- dihydroxy naphthlene 5.0mmol/L；
Solvent is ethyl alcohol
The formula of B reagent is as follows:
Solvent is purified water
It is cut to the test-paper of 12mm × 12mm size with strip cutting machine, is sealed.
Detection method: 10 μ L test samples are added dropwise in analoids, reaction after five minutes, is surveyed with baffled photometer
It is fixed, the concentration of hydrogen peroxide in sample is calculated according to standard curve.
Test example 3: the analysis of the accuracy of 2 the method for the embodiment of the present invention
Test sample: it purchases from 5 parts of the famous brand name Chicken Feet with Pickled Peppers sample of supermarket, 5 parts of famous brand name milk, famous brand name
5 parts of bubble green pepper crackling sample；By national food safety standard GB5009.226-2016 " measurement of residual quantities of hydrogen peroxide in food "
Method handle sample.
Verification method: the national food safety standard GB 5009.226-2016 " survey of residual quantities of hydrogen peroxide in food is pressed
Method calmly " is verified.
Dry plate detection reagent card using the detection method of embodiment 2 to above-mentioned sample in embodiment 2 detects, detection
It the results are shown in Table 4.
Table 4 analyzes result
The results show that the detection knot of the testing result and GB 5009.226-2016 obtained according to 2 detection method of embodiment
Fruit is consistent, it was demonstrated that practicability and consistency of the invention.
Test example 4: the Precision Analyze of 2 the method for the embodiment of the present invention
Test sample: 50mg/L hydrogen peroxide liquid sample；
Detection 10 times is repeated to same sample to be tested with embodiment 2 and 3 detection method of comparative example, testing result is shown in Table 5,6.
5 embodiment of table, 2 test sample concentration of hydrogen peroxide (10 times) and standard rate (coefficient of variation)
6 comparative example of table, 3 test sample concentration of hydrogen peroxide (10 times) and standard rate (coefficient of variation)
The results show that 2 standard rate of embodiment is 2.51%, less than the 10% of standard requirements.3 standard rate of comparative example is
7.07%, hence it is evident that be greater than 2 standard rate 2.51% of embodiment, show that the method for the invention has high precision.
Test example 5: 2 the method for the embodiment of the present invention and 109507176 A Patent Reference of application publication number CN analyze
1, Precision Analyze
Test sample: 20mg/L hydrogen peroxide liquid sample；
With 2 detection method of embodiment and 109507176 A patent detection method of application publication number CN to same sample to be tested
Detection 10 times is repeated, testing result is shown in Table 7,8.
7 embodiment of table, 2 test sample concentration of hydrogen peroxide (10 times) and standard rate (coefficient of variation)
8 application publication number CN of table, 109507176 A patent test sample concentration of hydrogen peroxide (10 times) and standard rate
(coefficient of variation)
2, linear analysis and detection lower bound analysis
Test sample: high-strength hydrogen peroxide sample (500mg/L)；
Hydrogen peroxide sample is diluted to 12 different concentration, be followed successively by 0mg/L, 10mg/L, 20mg/L, 30mg/L,
40mg/L、50mg/L、60mg/L、100mg/L、200mg/L、300mg/L、400mg/L、500mg/L。
Using embodiment 2 detection method to 0mg/L, 50mg/L, 100mg/L, 200mg/L, 300mg/L, 400mg/L,
500mg/L concentration of hydrogen peroxide sample carries out 3 detections, calculates coefficient R value, and testing result is shown in Table 9.
Using the detection method of 109507176 A patent of application publication number CN to 0mg/L, 10mg/L, 20mg/L, 30mg/
L, 40mg/L, 50mg/L, 60mg/L concentration of hydrogen peroxide sample carry out 3 detections, calculate coefficient R value, and testing result is shown in
Table 10.
The dry plate detection reagent card line test result of 9 embodiment 2 of table preparation
The standby dry chemical detection lug linear test result of 10 application publication number CN of table, 109507176 A patent system
The results show that 109507176 A patented criteria rate of application publication number CN is 7.63%, hence it is evident that be greater than embodiment 2
Standard rate 3.28%.109507176 A patent dry chemical detection lug of application publication number CN is in the range of 3mg/L-50mg/L
With the good linearity, 2 dry plate detection reagent of embodiment is stuck in the range of 0.3mg/L-400mg/L with good linear
Degree；Show that the method for the invention has higher precision, the range of linearity than 109507176 A patent of application publication number CN
With lower detection lower bound.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of dry plate detection reagent card of quantitative detection concentration of hydrogen peroxide, which is characterized in that the dry plate detection reagent card
Comprising three laminations, it is followed successively by support layer, color layer and diffusion layer；
Reagent in the color layer includes hydrophilic colloid, surfactant, chromogen A, chromogen B, chromogen C；The chromogen A is
4-AA, the chromogen B be 1,7- dihydroxy naphthlene, the chromogen C be selected from phenol, parachlorphenol, P-hydroxybenzoic acid,
One of 3,5- dichloro sodium hydroxybenzenesulfonate；
Reagent in the diffusion layer includes hydrophilic high molecular polymer, surfactant, particulate matter, secondary heme six
Peptide.
2. dry plate detection reagent card according to claim 1, which is characterized in that the reagent dosage in the color layer is such as
Under:
3. dry plate detection reagent card according to claim 1 or 2, which is characterized in that the hydrophilic colloid is selected from poly- second
Enol, amylopectin, xanthan gum, sodium alginate, gum arabic, peach gum, chitosan, cellulose esters, polyvinylpyrrolidone,
Polyacrylamide, hydroxypropyl methyl cellulose, sodium carboxymethylcellulose, one in Copolymer of Methyl Vinyl Ether/Maleic Anhydride
Kind is several.
4. dry plate detection reagent card according to claim 1, which is characterized in that the reagent dosage in the diffusion layer is such as
Under:
5. dry plate detection reagent card according to claim 1 or 4, which is characterized in that the hydrophilic high molecular polymer
Selected from polyurethane, polyamide, cellulose acetate, nitrocellulose, polyvinyl alcohol, polyvinylpyrrolidone, polyvinyl alcohol contracting fourth
One of aldehyde, amylopectin, xanthan gum, sodium alginate, gum arabic, peach gum, chitosan, polyvinylacetate are several
Kind.
6. dry plate detection reagent card according to claim 1 or 4, which is characterized in that the particulate matter is selected from titanium dioxide
One or more of titanium, barium sulfate, crystallite colloid, silica, resin bead, bead, diatomite, cellulose esters.
7. according to claim 1, dry plate detection reagent card described in any one of 2 or 4, which is characterized in that the surface-active
Agent is sorbitanmonolaureate, polyoxyethylene sorbitan monooleate 20, polyethylene glycol in isooctyl phenyl ether
One or more.
8. dry plate detection reagent card according to claim 1, which is characterized in that the support layer is poly terephthalic acid second
The polymer of diol ester, with a thickness of 50~300 microns.
9. the preparation method of dry plate detection reagent card described in any one of claims 1 to 8, which is characterized in that including walking as follows
It is rapid:
Developing solution reagent is coated on support layer, is dried for the first time；Diffusion liquid reagent is sprayed on support layer, is dried again,
Obtain include support layer, color layer and diffusion layer dry plate detection reagent card.
10. preparation method according to claim 9, which is characterized in that the temperature dry for the first time is 40~60 DEG C, first
The time of secondary drying is 3~5min；The temperature dried again is 40~50 DEG C, and the dry time is 5~10min again.
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| CN112505031A (en) * | 2020-12-04 | 2021-03-16 | 上海碧云天生物技术有限公司 | Novel detection method of aldehyde substance and application thereof |
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