CN110129893A - A kind of preparation method and applications of chip - Google Patents
A kind of preparation method and applications of chip Download PDFInfo
- Publication number
- CN110129893A CN110129893A CN201910228224.0A CN201910228224A CN110129893A CN 110129893 A CN110129893 A CN 110129893A CN 201910228224 A CN201910228224 A CN 201910228224A CN 110129893 A CN110129893 A CN 110129893A
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- chip
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- preparation
- nucleic acid
- silicon dioxide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The present invention provides a kind of preparation method and applications of chip, the chip includes hypothallus, silicon wafer layer and silicon dioxide layer, containing different nucleic acid fragment point samples on chip, passes through mass spectral analysis precise measurement target dna sequence.The chip can measure nucleic acid molecules (A, T, C, G), nucleic acid small fragment；Other small molecules, such as amino acid, urea small molecule compound can also be measured simultaneously.Detection for nucleic acid fragment and other small molecules is provided convenience.Chip detection simply, conveniently, can be realized high-throughput detection, be with a wide range of applications.
Description
Technical field
The present invention relates to biochip field, in particular to a kind of nucleic acid mass spectrum chip and preparation method thereof and application.
Background technique
The present invention relates to biochip field, in particular to a kind of nucleic acid mass spectrum chip and preparation method thereof and application, institute
Stating nucleic acid mass spectrum chip includes hypothallus, silicon wafer layer and silicon dioxide layer；The material requested for preparing of the nucleic acid mass spectrum chip is
The silicon wafer and hydroxy-picolinic acid of high-purity, by the preparation of wafer, wafer photolithography development, etching form silicon dioxide layer, hydroxyl
Yl pyridines formic acid is prepared made from three steps of coating；Nucleic acid mass spectrum chip manufacturing of the invention is simple, low manufacture cost.This hair
The nucleic acid mass spectrum chip of bright offer may be implemented on nucleic acid mass spectrograph, and after point sample, by laser, nucleic acid fragment in the electric field, leads to
The difference of charge-mass ratio is crossed, the nucleic acid fragment of different molecular weight size is separated.
The separation that the present invention is intended to be used in nucleic acid molecules on nucleic acid mass spectrum, lays a good foundation thus for Genotyping.
Summary of the invention
It is an object of the invention to develop a kind of nucleic acid mass spectrum chip, it is mainly used for nucleic acid molecules or other small molecules
It detects and separates.
To achieve the goals above, the present invention the following technical schemes are provided:
A kind of chip, the chip includes hypothallus, silicon wafer layer and silicon dioxide layer, containing different nucleic acid fragment point samples in core
On piece passes through mass spectral analysis precise measurement target dna sequence.
The preparation method of chip of the present invention, comprising the following steps:
(1) basal layer of the preparation for chip preparation, is silicon wafer layer, and the purity of silicon is 99.999%；
It (2) is silicon dioxide layer in preparing middle layer on basal layer, silicon dioxide layer is developed by wafer photolithography, etches shape
At silicon dioxide layer；
It (3) is hypothallus in preparing superficial layer in middle layer, matrix layer formula is hydroxy-picolinic acid.
Chip of the invention includes hypothallus, silicon wafer layer and silicon dioxide layer.The purity of silicon dioxide layer, silicon is
99.999%.It is silicon dioxide layer for being good for layer in chip preparation, silicon dioxide layer is developed by wafer photolithography, etching is formed
Silicon dioxide layer；Superficial layer for chip preparation is hypothallus, and matrix layer formula is hydroxy-picolinic acid.
Preferably, there is everybody 200 μm of hypothallus of 96 length and width on the silicon wafer layer.
Preferably, there is each 40 μm of a length and width of small frame block among two neighboring hypothallus, be used for laser alignment.
Application of the above-described chip in nucleic acid molecules or amino acid, the detection of urea small molecule.
The beneficial effects of the present invention are: the present invention provides a kind of preparation method and applications of chip, which can be with
It measures nucleic acid molecules (A, T, C, G), nucleic acid small fragment；Other small molecules, such as small point of amino acid, urea can also be measured simultaneously
Sub- compound.Detection for nucleic acid fragment and other small molecules is provided convenience.Chip detection simply, conveniently, can be realized
High throughput detection, is with a wide range of applications.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of chip of the invention.
Fig. 2 is the constituent analysis figure of chip hydroxy-picolinic acid of the present invention.
Fig. 3 is the constituent analysis figure of chip silica of the present invention.
Fig. 4 is the constituent analysis figure of chip high purity silicon of the present invention.
Fig. 5 is the result analysis chart for detecting HPV.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
A kind of chip, the chip include hypothallus, silicon wafer layer and silicon dioxide layer, contain different nucleic acid fragment point samples
On chip, pass through mass spectral analysis precise measurement target dna sequence.
The preparation method of chip of the present invention, comprising the following steps:
(1) basal layer of the preparation for chip preparation, is silicon wafer layer, and the purity of silicon is 99.999%；
It (2) is silicon dioxide layer in preparing middle layer on basal layer, silicon dioxide layer is developed by wafer photolithography, etches shape
At silicon dioxide layer；
It (3) is hypothallus in preparing superficial layer in middle layer, matrix layer formula is hydroxy-picolinic acid.
Fig. 1 is the structural schematic diagram of chip of the invention, and 200 μm of black of circle is hypothallus in figure, 40 μm of silver color
Circle is used for laser alignment, and bottom is silicon wafer.
Table 1 is the constituent analysis table of chip of the present invention, and #1 is hydroxy-picolinic acid in table 1, and #2 is silica, and #3 is height
Purity silicon, corresponding component content difference are as shown in Figure 2,3, 4.
Table 1
Chip of the invention includes hypothallus, silicon wafer layer and silicon dioxide layer.Silicon dioxide layer, the purity of silicon are 99.999%.
It is silicon dioxide layer for being good for layer in chip preparation, silicon dioxide layer is developed by wafer photolithography, etching forms silica
Layer；Superficial layer for chip preparation is hypothallus, and matrix layer formula is hydroxy-picolinic acid.
There is everybody 200 μm of hypothallus of 96 length and width on the silicon wafer layer.
There is each 40 μm of a length and width of small frame block among two neighboring hypothallus, is used for laser alignment.
The chip prepared with this method, detects HPV, and result is as shown in Figure 5.
Claims (5)
1. a kind of chip, which is characterized in that the chip includes hypothallus, silicon wafer layer and silicon dioxide layer, contains different core
Acid fragment point sample passes through mass spectral analysis precise measurement target dna sequence on chip.
2. the preparation method of chip described in claim 1, which comprises the following steps:
(1) basal layer of the preparation for chip preparation, is silicon wafer layer, and the purity of silicon is 99.999%；
It (2) is silicon dioxide layer in preparing middle layer on basal layer, silicon dioxide layer is developed by wafer photolithography, etches shape
At silicon dioxide layer；
It (3) is hypothallus in preparing superficial layer in middle layer, matrix layer formula is hydroxy-picolinic acid.
3. preparation method according to claim 2, which is characterized in that have on the silicon wafer layer 96 length and width everybody 200 μm
Hypothallus.
4. preparation method according to claim 3, which is characterized in that there is length and width each 40 among two neighboring hypothallus
μm small frame block, be used for laser alignment.
5. application of the chip described in claim 1 in nucleic acid molecules or amino acid, the detection of urea small molecule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910228224.0A CN110129893A (en) | 2019-03-25 | 2019-03-25 | A kind of preparation method and applications of chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910228224.0A CN110129893A (en) | 2019-03-25 | 2019-03-25 | A kind of preparation method and applications of chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110129893A true CN110129893A (en) | 2019-08-16 |
Family
ID=67568832
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201910228224.0A Pending CN110129893A (en) | 2019-03-25 | 2019-03-25 | A kind of preparation method and applications of chip |
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| CN (1) | CN110129893A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6300076B1 (en) * | 1995-03-17 | 2001-10-09 | Sequenom, Inc. | DNA diagnostics based on mass spectrometry |
| CN101965221A (en) * | 2008-01-15 | 2011-02-02 | 塞昆纳姆股份有限公司 | Compositions and processes for improved mass spectrometry analysis |
| CN107192757A (en) * | 2017-07-05 | 2017-09-22 | 北京毅新博创生物科技有限公司 | A kind of dual-purpose detection kit of mass spectrum |
| CN108008002A (en) * | 2017-10-31 | 2018-05-08 | 北京毅新博创生物科技有限公司 | Correct the method and product of the accuracy rate of Mass Spectrometer Method nucleic acid samples |
-
2019
- 2019-03-25 CN CN201910228224.0A patent/CN110129893A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6300076B1 (en) * | 1995-03-17 | 2001-10-09 | Sequenom, Inc. | DNA diagnostics based on mass spectrometry |
| CN101965221A (en) * | 2008-01-15 | 2011-02-02 | 塞昆纳姆股份有限公司 | Compositions and processes for improved mass spectrometry analysis |
| CN107192757A (en) * | 2017-07-05 | 2017-09-22 | 北京毅新博创生物科技有限公司 | A kind of dual-purpose detection kit of mass spectrum |
| CN108008002A (en) * | 2017-10-31 | 2018-05-08 | 北京毅新博创生物科技有限公司 | Correct the method and product of the accuracy rate of Mass Spectrometer Method nucleic acid samples |
Non-Patent Citations (1)
| Title |
|---|
| 有机质谱专业委员会: "《现代有机质谱技术及应用》", 31 August 1999, 中国人民公安大学出版社 * |
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