CN110129824A - A kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol - Google Patents
A kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol Download PDFInfo
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- CN110129824A CN110129824A CN201910536884.5A CN201910536884A CN110129824A CN 110129824 A CN110129824 A CN 110129824A CN 201910536884 A CN201910536884 A CN 201910536884A CN 110129824 A CN110129824 A CN 110129824A
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- ergosterol
- sparassis crispa
- electric field
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- field preparation
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- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 title claims abstract description 51
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 title claims abstract description 51
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 title claims abstract description 51
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 title claims abstract description 51
- 241000272503 Sparassis radicata Species 0.000 title claims abstract description 51
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 33
- 230000005684 electric field Effects 0.000 title claims abstract description 30
- 235000001968 nicotinic acid Nutrition 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000000243 solution Substances 0.000 claims abstract description 26
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 23
- 239000012141 concentrate Substances 0.000 claims abstract description 16
- 235000019441 ethanol Nutrition 0.000 claims abstract description 15
- 238000007127 saponification reaction Methods 0.000 claims abstract description 15
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- 239000003963 antioxidant agent Substances 0.000 claims abstract description 8
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- 238000002390 rotary evaporation Methods 0.000 claims abstract description 8
- -1 that is Substances 0.000 claims abstract description 8
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 7
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- 238000000926 separation method Methods 0.000 claims abstract description 4
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- 230000008021 deposition Effects 0.000 claims abstract description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 3
- 229930182558 Sterol Natural products 0.000 claims description 3
- 229910052804 chromium Inorganic materials 0.000 claims description 3
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- 150000003432 sterols Chemical class 0.000 claims description 3
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052785 arsenic Inorganic materials 0.000 claims description 2
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 claims description 2
- 229910002804 graphite Inorganic materials 0.000 claims description 2
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- 239000003208 petroleum Substances 0.000 description 4
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 3
- 229920002498 Beta-glucan Polymers 0.000 description 3
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
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- 238000004925 denaturation Methods 0.000 description 2
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- 230000000968 intestinal effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
- WURBVZBTWMNKQT-UHFFFAOYSA-N 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1,2,4-triazol-1-yl)butan-2-one Chemical group C1=NC=NN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 WURBVZBTWMNKQT-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000003792 Metallothionein Human genes 0.000 description 1
- 108090000157 Metallothionein Proteins 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- MECHNRXZTMCUDQ-UHFFFAOYSA-N Vitamin D2 Natural products C1CCC2(C)C(C(C)C=CC(C)C(C)C)CCC2C1=CC=C1CC(O)CCC1=C MECHNRXZTMCUDQ-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 230000003026 anti-oxygenic effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 229960002061 ergocalciferol Drugs 0.000 description 1
- DNVPQKQSNYMLRS-APGDWVJJSA-N ergosterol group Chemical group [C@@H]1(CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C)[C@H](C)\C=C\[C@H](C)C(C)C DNVPQKQSNYMLRS-APGDWVJJSA-N 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 208000005368 osteomalacia Diseases 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- 150000003254 radicals Chemical class 0.000 description 1
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- 238000005057 refrigeration Methods 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
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- 238000011105 stabilization Methods 0.000 description 1
- MECHNRXZTMCUDQ-RKHKHRCZSA-N vitamin D2 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)/C=C/[C@H](C)C(C)C)=C\C=C1\C[C@@H](O)CCC1=C MECHNRXZTMCUDQ-RKHKHRCZSA-N 0.000 description 1
- 235000001892 vitamin D2 Nutrition 0.000 description 1
- 239000011653 vitamin D2 Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
-
- C—CHEMISTRY; METALLURGY
- C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
- C25B—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES FOR THE PRODUCTION OF COMPOUNDS OR NON-METALS; APPARATUS THEREFOR
- C25B3/00—Electrolytic production of organic compounds
- C25B3/20—Processes
- C25B3/25—Reduction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Materials Engineering (AREA)
- Metallurgy (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The method of-half bionics techniques of a kind of electric field preparation Sparassis crispa ergosterol disclosed by the invention, comprising the following steps: (1) take Sparassis crispa fructification, be ground up, sieved, obtain Sparassis crispa powder；(2) under the conditions of external electric field, using pH for 9-10 strong base solution as raw material, saponification at 90 DEG C obtains ergosterol；(3) heavy metal ion desorbed in step described in electroreduction (2) by albuminate；(4) gained mixed liquor in the step (3) is taken, centrifuge separation obtains layer deposition object, middle layer flocculate and supernatant；(5) the middle layer flocculate is taken, using the aqueous solution of ethyl alcohol as extracting solution, weak acid for adjusting pH to 3-4, water-bath is extracted, and filtering obtains filtrate；(6) merge supernatant and filtrate, adjust pH to neutrality, rotary evaporation obtains concentrate, concentrate described in vacuum freeze drying, gained powder, that is, anti-oxidant Sparassis crispa ergosterol, ergosterol recovery rate are high, removing heavy metals and silk ball mycoprotein are removed, property is stablized, through gastrointestinal tract good absorbing.
Description
Technical field
The present invention relates to Sparassis crispa fields, and in particular to a kind of-half bionics techniques of electric field preparation Sparassis crispa ergosterol
Method.
Background technique
Ergosterol is also known as ergot and stays alcohol, is a kind of feature alcohol contained in fungi, the silk ball bacterial content in edible mushroom
It is big and stable, frequently as the primary raw material of calciferol production, it can promote absorption of the human body to pin, phosphorus etc., be conducive to bone shape
At, prevention children rachitis, adult osteoporosis and osteomalacia, meanwhile, ergosterol is the weight of microbial cell film
Component part is wanted, to the integrality for ensuring cell membrane, the activity of membrane bound enzyme, the mobility of film, cell viability and cell object
Matter transport etc. plays an important role, but it meets daylight and the oxidizable yellowly of air, property are unstable.
In the prior art, to the extraction of ergosterol in mostly organic solvent extractionprocess, while extracting ergosterol
Also a large amount of foreign matters are extracted, not only consume a large amount of organic solvent, and since ergosterol finally plays correlation by oral administration
Health-care effect, organic solvent extractionprocess is extract obtained bad to the adaptability of human body intestinal canal, simultaneously as edible mushroom is easily enriched with
Heavy metal, thus through organic solvent extract content of beary metal in resulting extract be also possible to it is exceeded.
Summary of the invention
To solve the above problems, the present invention provides a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol,
Ergosterol recovery rate is high, through electric field-semi-bionic extraction method, mixes Sparassis crispa polysaccharide component, removes removing heavy metals and silk ball mycoprotein,
Property stabilization is not oxidizable, through gastrointestinal tract good absorbing.
The technical scheme is that a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol is provided,
The following steps are included: (1) takes Sparassis crispa fructification, it is ground up, sieved, obtains Sparassis crispa powder；It (2) is 9- with pH under the conditions of external electric field
10 strong base solution is raw material, and saponification at 90 DEG C obtains ergosterol；(3) by being denaturalized in step described in electroreduction (2)
The heavy metal ion of albumen desorption；(4) take gained mixed liquor in the step (3), centrifuge separation, obtain layer deposition object, in
Fleece condensate and supernatant；(5) the middle layer flocculate is taken, using the aqueous solution of ethyl alcohol as extracting solution, weak acid for adjusting pH to 3-4, water
Bath is extracted, and filtering obtains filtrate；(6) merge supernatant and filtrate, adjust pH to neutrality, rotary evaporation obtains concentrate, and vacuum refrigeration is dry
The dry concentrate, gained powder, that is, anti-oxidant Sparassis crispa ergosterol.
Preferably, in the step (3), the cathode of electroreduction is stainless steel electrode plate, and anode is graphite electrode plate.
Preferably, in the step (2), external electric field electric field strength is 20-40kv/cm, and pulse is 6 μ s-12 μ s, feed liquid
Than for 1:20.
Preferably, the highly basic in the step (2) is one of NaOH or KOH.
Preferably, the weak acid in the step (5) is acetic acid.
Preferably, in the step (4), centrifugal speed 3000-4000r/m, centrifugation time 2-3min.
Preferably, the heavy metal ion in the step (3) is one or more of chromium, lead, arsenic.
Preferably, the volume ratio of ethyl alcohol and water is 2:8 in extracting solution in the step (5).
High-pressure pulse electric is placed in container using food liquid as electrolyte, two discharge electrodes with container edge
By high-tension current, the method that electric pulse is processed is generated, the cell wall and cell membrane potential of cell processed can be made moment
Disorder changes its permeability, or even can puncture cell wall and cell membrane, brings it about irreversible breaking, and group distributes in cell.
In Sparassis crispa fructification there are two types of ergosterol existences, free state and reference state, the prior art are generally first adopted
It is saponified with alcohol alkali, the purpose being saponified to mycelium is the fatty acid removed in mycelium, is added in lye rudimentary
It is so that grease and alkali alcoholic solution is formed homogeneous reaction system in order to increase grease solubility in saponification liquor that alcohol, which makees saponification agent, from
And the reaction rate of saponification is improved, the extractions such as organic solvent such as petroleum ether, ether wherein unsaponifiable matter is recycled, but this
Method not only used a large amount of organic solvent, also will use the organic solvents such as ether or petroleum ether in subsequent extraction process
Extractant is made, there is very big security risk in these organic solvents, and cost is also very high in industrialized production, additionally, due to
Ergosterol also is soluble in ethyl alcohol, therefore the recovery rate that can lose ergosterol is taken with alcohol alkali carries, ring of this programme in impulse electric field
Under border, ergosterol is saponified using aqueous slkali, Electro-pulsing Field is in Sparassis crispa solution, so that oscillation aggravation, wheat when reaction
Angle sterol is constantly dissolved out with the destruction of pulse pair cell membrane, is avoided the interface of saponification between the two phases from carrying out, is accelerated alkali
Reference state ergosterol in mycelia can be become free state, avoid subsequent extraction process by liquid to the diffusion rate of grease
Extractant is made using organic solvents such as ether or petroleum ethers, the safety that ergosterol extracts is improved, in addition also makes production cost
Greatly reduce.
The extraction of this programme also imitates the first lye that human gastrointestinal tract is absorbed and carried out and mentions again acid solution i.e. semi-bionic extraction method, alkali
The pH value of liquid is functioned simultaneously as close to human body gastric acid using the lye in saponification close to human body intestinal canal, the pH value of acid solution
Extractant extracts Sparassis crispa active constituent, as silk ball granulose increases its solubility ratio and examine for oral absorption
Consider that degree of dissociation is more important, and silk ball granulose includes beta glucan, with antioxygenic property, and free radical can be removed, it is rear with containing
The acid solution of ethyl alcohol extracts purifying to ergosterol, enhances its dissolubility, and with the extracted ergosterol of this programme, there are also big
The beta glucan of amount, when external condition causes the unstable oxidation of ergosterol, beta glucan can be first oxidized, and protect etembonate
Alcohol, thus keep its property more stable.
After carrying out lye and extracting the effect with impulse electric field, since saponification temperature is higher than 80 DEG C, i.e. the denaturation of protein
Temperature, and the pH of lye, but also Sparassis crispa albuminous degeneration is known as flocculate precipitating, silk ball mycoprotein includes sulfoprotein, in silk ball
Since there are heavy metal pollutions in growing environment during bacterium growth, and as macro fungi, Sparassis crispa is easily enriched with a huge sum of money
Belong to, especially chromium, since sulfoprotein is rich in the small peptide of cysteine, has high affinity to various heavy, it is molecule matter
To measure lower, cysteine residues and the high protein of tenor form metallothionein, after protein can be denaturalized, a huge sum of money
Category is parsed with the denaturation of sulfoprotein, to be dissolved in solution in ionic state heavy metal, electrolytic reduction is utilized in this programme
Enriching heavy metal is inserted into electrode in the sewage containing heavy metal, then passes to the direct current of some strength, heavy metal is in electric field
Lower displacement is acted on, heavy metal simple substance is precipitated on the electrode or is enriched near electrode, to remove heavy metal, in cathode,
Due to the difference of heavy metal ion electrode potential, heavy metal ion is reduced to heavy metal simple substance in cathode, and mixed liquor is through being centrifuged
Afterwards, since heavy metal density is larger, bottommost is fallen to, and the Sparassis crispa residue containing ergosterol forms flocculate and falls to
Middle part, and then carry out the separation and removal of heavy metal.
This programme has the beneficial effect that:
(1) highly basic is that the extractant for being used as semi-bionic extraction method does not influence also as the saponification agent for generating ergosterol in this programme
In the case where semi-bionic extraction method process, ergosterol is extracted；
(2) electric field destroys Sparassis crispa cell membrane, so that the ergosterol in cell membrane dissolves out, improves semi-bionic extraction method rate, simultaneously
Electric field acceleration lye accelerates the progress of saponification to the diffusion rate of grease, keeps its reaction more thorough；
(3) while electric field-saponification, keep silk ball mycoprotein rotten, parse the heavy metal in conjunction with sulfoprotein, recycle original
Electric field environment, electroreduction is enriched with heavy metal, obtains free of contamination Sparassis crispa ergosterol extract；
(4) due to utilizing semi-bionic extraction method, the loss of effective component is reduced, not only with respect to the activity of mixture in extraction process
Ingredient, also controls the monomer component to be extracted, and in Sparassis crispa ergosterol obtained, granulose containing silk ball can prevent wheat
Angle sterol aoxidizes, and enhances the stability of ergosterol.
Detailed description of the invention
Fig. 1 is Determination of ergosterol testing result；
Fig. 2 is ergosterol Detection of Stability result；
Fig. 3 is content of beary metal testing result.
Specific embodiment
In order to make content of the present invention easily facilitate understanding, With reference to embodiment to of the present invention
Technical solution is described further, but the present invention is not limited only to this.
Embodiment 1
Sparassis crispa fructification 100g is taken, is ground, 200 meshes are crossed, obtained Sparassis crispa powder is packed into high-pressure pulse device,
NaOH solution 2L is added, adjustment solution ph is heating stirring 1h at 9,90 DEG C, the condition of micro-electrolysis reactor is adjusted, so that outside
Electric field intensity is 20kv/cm, and pulse is that 6 μ s occur simultaneously so that discharge electrode acts on Sparassis crispa cell membrane
Saponification；
The mixed liquor is poured into micro-electrolysis reactor, adjusts the electrode, so that cathode is stainless steel electrode plate, anode is stone
Electrode ink plate carries out electroreduction, voltage 1.8V to the resulting mixed liquor of above-mentioned steps, and reaction temperature is 25 DEG C, when reaction
Between be that 120min is centrifuged resulting mixed liquor, centrifugal speed 3000r/m after slowly removing cathode electrode, when centrifugation
Between be 2min, leave and take supernatant and the i.e. middle layer flocculate of first layer precipitating；
The middle layer flocculate is taken, 200ml ethyl alcohol is added, 800ml acetum adjusts pH to 3, and 1h, mistake are extracted in 40 DEG C of water-baths
Filter, takes filtrate；
Merging filtrate and supernatant adjust pH to neutrality, and rotary evaporation obtains concentrate, concentrate described in vacuum freeze drying, gained
Powder, that is, anti-oxidant Sparassis crispa ergosterol takes gained powder to carry out Data Detection.
Embodiment 2
Sparassis crispa fructification 100g is taken, is ground, 200 meshes are crossed, obtained Sparassis crispa powder is packed into high-pressure pulse device,
KOH solution 2L is added, adjustment solution ph is heating stirring 1h at 10,90 DEG C, the condition of micro-electrolysis reactor is adjusted, so that outside
Electric field intensity is 40kv/cm, and pulse is that 12 μ s occur simultaneously so that discharge electrode acts on Sparassis crispa cell membrane
Saponification；
The mixed liquor is poured into micro-electrolysis reactor, adjusts the electrode, so that cathode is stainless steel electrode plate, anode is stone
Electrode ink plate carries out electroreduction, voltage 1.8V to the resulting mixed liquor of above-mentioned steps, and reaction temperature is 25 DEG C, when reaction
Between be that 120min is centrifuged resulting mixed liquor, centrifugal speed 4000r/m after slowly removing cathode electrode, when centrifugation
Between be 3min, leave and take supernatant and the i.e. middle layer flocculate of first layer precipitating；
The middle layer flocculate is taken, 200ml ethyl alcohol is added, 800ml acetum adjusts pH to 4, and 1h, mistake are extracted in 40 DEG C of water-baths
Filter, takes filtrate；
Merging filtrate and supernatant adjust pH to neutrality, and rotary evaporation obtains concentrate, concentrate described in vacuum freeze drying, gained
Powder, that is, anti-oxidant Sparassis crispa ergosterol takes gained powder to carry out Data Detection.
Embodiment 3
Sparassis crispa fructification 100g is taken, is ground, 200 meshes are crossed, obtained Sparassis crispa powder is packed into high-pressure pulse device,
KOH solution 2L is added, adjustment solution ph is heating stirring 1h at 10,90 DEG C, the condition of micro-electrolysis reactor is adjusted, so that outside
Electric field intensity is 50kv/cm, and pulse is that 10 μ s occur simultaneously so that discharge electrode acts on Sparassis crispa cell membrane
Saponification；
The mixed liquor is poured into micro-electrolysis reactor, adjusts the electrode, so that cathode is stainless steel electrode plate, anode is stone
Electrode ink plate carries out electroreduction, voltage 1.8V to the resulting mixed liquor of above-mentioned steps, and reaction temperature is 25 DEG C, when reaction
Between be that 120min is centrifuged resulting mixed liquor, centrifugal speed 3500r/m after slowly removing cathode electrode, when centrifugation
Between be 3min, leave and take supernatant and the i.e. middle layer flocculate of first layer precipitating；
The middle layer flocculate is taken, 200ml ethyl alcohol is added, 800ml acetum adjusts pH to 3.5, and 1h is extracted in 40 DEG C of water-baths,
Filtering, takes filtrate；
Merging filtrate and supernatant adjust pH to neutrality, and rotary evaporation obtains concentrate, concentrate described in vacuum freeze drying, gained
Powder, that is, anti-oxidant Sparassis crispa ergosterol takes gained powder to carry out Data Detection.
Embodiment 4
Sparassis crispa fructification 100g is taken, is ground, 200 meshes are crossed, KOH solution 2L is added, adjustment solution ph is 10,90 DEG C
Lower heating stirring 1h, is centrifuged resulting mixed liquor, centrifugal speed 3500r/m, and centrifugation time 3min takes supernatant
200ml ethyl alcohol is added in above-mentioned precipitating in liquid, and 800ml acetum adjusts pH to 3.5, and 40 DEG C of water-baths are extracted 1h, filtered,
Take filtrate；
Merging filtrate and supernatant adjust pH to neutrality, and rotary evaporation obtains concentrate, concentrate described in vacuum freeze drying, gained
Powder, that is, anti-oxidant Sparassis crispa ergosterol takes gained powder to carry out Data Detection.
Embodiment 5
Sparassis crispa fructification 100g is taken, is ground, 200 meshes are crossed, KOH solution 2L is added in obtained Sparassis crispa powder, is adjusted
Whole solution ph is heating stirring 1h at 10,90 DEG C, and with petroleum ether extraction, rotary evaporation obtains concentrate, vacuum freeze drying institute
Concentrate is stated, gained powder, that is, anti-oxidant Sparassis crispa ergosterol takes gained powder to carry out Data Detection.
Embodiment 6
Data Detection is carried out according to known method to powder obtained by above-described embodiment 1-5, above-mentioned powder is dissolved in 1L solution
It is detected, Determination of ergosterol testing result such as Fig. 1, the measurement ergosterol of the powder obtained by illumination and oxidizer treatment is stablized
Property testing result such as Fig. 2, content of beary metal testing result such as Fig. 3, it is known that the resulting ergosterol recovery rate of this programme is high, content
High and property is stablized, heavy metal free residual.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (8)
1. a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol, which comprises the following steps:
(1) Sparassis crispa fructification is taken, is ground up, sieved, Sparassis crispa powder is obtained；
(2) under the conditions of external electric field, using pH for 9-10 strong base solution as raw material, saponification at 90 DEG C obtains free state ergot
Sterol；
(3) heavy metal ion desorbed in step described in electroreduction (2) by albuminate；
(4) gained mixed liquor in the step (3) is taken, centrifuge separation obtains layer deposition object, middle layer flocculate and supernatant；
(5) the middle layer flocculate is taken, using the aqueous solution of ethyl alcohol as extracting solution, weak acid for adjusting pH to 3-4, water-bath is extracted, filtering,
Obtain filtrate；
(6) merge supernatant and filtrate, adjust pH to neutrality, rotary evaporation obtains concentrate, concentrate described in vacuum freeze drying, institute
Obtain powder, that is, anti-oxidant Sparassis crispa ergosterol.
2. a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol according to claim 1, feature exist
In in the step (3), the cathode of electroreduction is stainless steel electrode plate, and anode is graphite electrode plate.
3. a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol according to claim 1, feature exist
In in the step (2), external electric field electric field strength is 20-40kv/cm, and pulse is 6 μ s-12 μ s, solid-liquid ratio 1:20.
4. a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol according to claim 1, feature exist
In the highly basic in the step (2) is one of NaOH or KOH.
5. a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol according to claim 1, feature exist
In the weak acid in the step (5) is acetic acid.
6. a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol according to claim 1, feature exist
In, in the step (4), centrifugal speed 3000-4000r/m, centrifugation time 2-3min.
7. a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol according to claim 1, feature exist
In the heavy metal ion in the step (3) is one or more of chromium, lead, arsenic.
8. a kind of method of-half bionics techniques of electric field preparation Sparassis crispa ergosterol according to claim 1, feature exist
In the volume ratio of ethyl alcohol and water is 2:8 in extracting solution in the step (5).
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110835149A (en) * | 2019-11-26 | 2020-02-25 | 上海天汉环境资源有限公司 | Method and device for removing heavy metal settlement through tubular column type electric flocculation |
| CN112679402A (en) * | 2021-01-25 | 2021-04-20 | 福建万菇生物科技有限公司 | Low-pollution sparassis crispa vitamin D2Preparation method of extract |
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| CN110835149A (en) * | 2019-11-26 | 2020-02-25 | 上海天汉环境资源有限公司 | Method and device for removing heavy metal settlement through tubular column type electric flocculation |
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