CN110129387A - The method that composite material immobilization propionic acid bar bacterium prepares niacinamide - Google Patents
The method that composite material immobilization propionic acid bar bacterium prepares niacinamide Download PDFInfo
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- CN110129387A CN110129387A CN201910313028.3A CN201910313028A CN110129387A CN 110129387 A CN110129387 A CN 110129387A CN 201910313028 A CN201910313028 A CN 201910313028A CN 110129387 A CN110129387 A CN 110129387A
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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Abstract
The present invention relates to a kind of preparation method of niacinamide, in particular to a kind of method that composite material immobilization propionic acid bar bacterium prepares niacinamide belongs to field of biotechnology.This method is will to be first cooled to room temperature after polyvinyl alcohol, polyethylene glycol add after water Hybrid Heating stirring to dissolve it sufficiently to obtain PVA-PEG hydrogel, sequentially add diatomite, active carbon is sufficiently stirred, and obtains immobilization composite material；Then to be fixedization thallus (wet thallus) is added in immobilization composite material, preparation PVA-PEG roundlet drop, by drying, Na2SO4It is multiple swollen in solution, immobilization sequin is collected, clear water is cleaned, cryo-conservation；Add substrate nicotinonitrile, obtains product nicotinamide after reaction.Method of the invention is small to microbial damage, immobilized cell high mechanical strength, long service life；Environmental pollution is small, separating-purifying is simple, product purity is high, is suitable for scale industrialized production.
Description
Technical field
The present invention relates to a kind of preparation method of niacinamide, in particular to a kind of composite material immobilization propionic acid bar bacterium system
The method of standby niacinamide, belongs to field of biotechnology.
Background technique
Niacinamide is also known as niacinamide (Nicotinamide), is white crystalline powder, odorless, bitter, dry shape
50 DEG C of state or less extremely stable, heats together with inorganic bronsted lowry acids and bases bronsted lowry, then can be hydrolyzed into niacin.Niacinamide is cozymase and codehydrogenase Ⅱ etc.
One of component part, participate in a variety of redox reactions of body.Mainly as nutritive additive (the water soluble vitamin life of feed
Element), it is also used for the intermediate of food, medicine, dyestuff, while being used as the additive and biochemical reagents of electroplate liquid.Niacinamide at present
Production technology has two major classes, and one kind is chemical method, and in addition one kind is biological synthesis process.Chemical method mainly uses 3- methyl pyrrole
Pyridine, 2- methyl-1,5- pentanediamine, 2- methyl -5- ethylpyridine etc. are initial feed, pass through oxidant direct oxidation or catalysis oxygen
Metaplasia hydrolyzes production niacinamide at nicotinonitrile, using lye.Chemical process comparative maturity, but catalyst, height need to be added
Temperature, multistep reaction, and catalyst is expensive, resulting reaction liquid hold-up is not high after reaction, and component is impure, it is unfavorable for extracting,
It is very big to the pollution of environment.Biological catalysis is to generate niacinamide using nitrile hydratase conversion nicotinonitrile.Microbial method is raw
Producing niacinamide has reaction condition mild, at low cost, products collection efficiency and purity is high, advantages of environment protection, these are all chemistry
Synthetic method is incomparable.Wherein bioanalysis can be divided into free cell catalysis and immobilized cell catalysis again.
Nitriles substance can all have for nitrile hydratase production bacterial strain or strong or weak toxicity.Microbial cell passes through immobilization
Afterwards, it is embedded in inside biomaterial, avoids and contacted with the direct of nitrile compounds, reduce nitrile compounds toxicity to micro-
The injury of biology, enables microbial cell to save conversion capability for a long time.Produce nitrilase microorganism immobilization with
Afterwards, the tolerance to product and substrate can be enhanced, improve continuous transformation ability.
Existing process for fixation has physisorphtion, covalent coupling method, cross-linking method and investment.Investment reaction condition
Mildly, the conformation and vigor for having higher embedding amount, capable of preferably maintaining enzyme, also contribute to the stability of enzyme.Although fixed
Change the serialization that cell is conducive to large-scale biotransformation, simplifies isolating and purifying for product, reduce production cost.But it passes
System immobilized cell form generally uses pellet form, exchanges with substrate not exclusively inside immobilized spherule, and mass transfer is obstructed, and influences
Reaction rate.
Summary of the invention
The present invention provides a kind of method that composite material immobilization propionic acid bar bacterium prepares niacinamide, this method technique letter
It is single, it is suitble to large-scale production, product yield is high, purity is high.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of method that composite material immobilization propionic acid bar bacterium prepares niacinamide, this method comprises the following steps:
1) immobilization of propionic acid bar bacterium:
(1) immobilization composite material:
Formula of the immobilization with composite material in terms of mass percentage are as follows:
Polyvinyl alcohol (PVA) 5%~20%,
Polyethylene glycol (PEG) 3%~10%,
Diatomite 0.1%~2%,
Active carbon 0.1%~1%,
Surplus is water, and the above constituent mass adds up to 100%；
Polyvinyl alcohol, polyethylene glycol add after water Hybrid Heating stirring dissolve it sufficiently to obtain PVA-PEG hydrogel, then successively add
Enter diatomite, be cooled to room temperature after active carbon is sufficiently stirred, obtains immobilization composite material；
(2) the to be fixedization thallus obtained by Liquid Culture is mixed with immobilization with composite material, is sufficiently stirred, makes thallus
It is uniformly mixed with immobilization with composite material；
(3) mixture that step (2) obtains is dripped on PE plate, forms the dot matrix of 3~6mm of diameter；
(4) drop for obtaining step (3) has the PE plate of sequin to be put into baking oven, its moisture content is made to be down to 10%~25%；
(5) the PE plate that step (4) obtains is placed in Na2SO4In aqueous solution, make the sequin on PE plate in Na2SO4In aqueous solution
Submergence；
(6) the PVA-PEG sequin of preparation, cryo-conservation after cleaning are collected；
2) preparation of niacinamide:
The immobilization sequin that above-mentioned steps obtain is added in the nicotinonitrile solution that mass concentration is 10~30%, it is fixed
The additional amount for changing sequin is 10-30%, obtains niacinamide after 60~240min of reaction time.3- cyano pyrrole is detected using HPLC
The residual quantity of pyridine and the production quantity of niacinamide.
The present invention prepares the immobilization sequin of platypelloid type using composite materials immobilization propionic acid bar bacteriums such as PVA-PEG,
Traditional investment prepares immobilized cell and mostly uses sodium alginate, polyvinyl alcohol, acrylamide, gelatin, chitosan and carboxymethyl
The materials such as cellulose do single embedding or two kinds of use, the combination of multiple material are done and meet embedding, and compound embedding techniques are not using
Same material mixing, gathers the advantage of different materials, makes up the deficiency of homogenous material.In above-mentioned different materials belong to sodium alginate and
It is researcher using more scheme that polyvinyl alcohol, which meets embedding techniques, and the immobilized spherule of preparation has preferable mechanical strong
Degree is, it can be achieved that multiple batches of applies, but since polyvinyl alcohol will form fine and close gel network in the curing process, is unfavorable for passing
The quick progress of matter.Polyethylene glycol material is introduced in the present invention, is conducive to reinforce mass transfer effect, is compensated for traditional immobilized spherule
The low disadvantage of vigor compared with free cell.Immobilization sequin obtained has higher mechanical strength simultaneously, and laboratory is answered
Immobilized cell obtained can realize that the hydrolysis not less than 40 batches is applied in.The process for fixation and form are for fixing third
Sour bar bacterium and the bioconversion for being used for nitrile compounds have very high industrial application value.
Preferably, the fluid nutrient medium of thallus forms in step (2) are as follows: 10~40g of glucose, 5~20g of yeast extract,
2~10g of urea, 1~5g of potassium dihydrogen phosphate, 1~5g of dipotassium hydrogen phosphate, 1~5g of magnesium sulfate, 2~6g of sodium glutamate, cobalt chloride
0.001~0.01g, adds water to 1L, and pH value is 6.8~7.5.Condition of culture are as follows: the culture medium of 250ml triangle bottled 10~30%,
1~10% strain is added after sterilizing, on shaking table with 100~300rpm constant-temperature shaking culture, 25~35 DEG C of cultivation temperature, training
Support 36~108h of time.
Preferably, the mass ratio of thallus and immobilization composite material is 0.1~1:10 in step (2).
Preferably, the oven temperature for declining PVA-PEG sequin moisture content in step (4) is arranged at 20-50 DEG C, dry
The dry time is 30-120min.
Preferably, in step (5), Na2SO4Mass percentage be 1~20%, the soaking time of sequin is 20~
40min。
Preferably, storage temperature is 2~10 DEG C in step (6).
Preferably, the polyethylene glycol is polyethylene glycol 200, polyethylene glycol 400, polyethylene glycol-800, polyethylene glycol
2000, any one in Macrogol 4000；The polyvinyl alcohol is polyvinyl alcohol 1799, one in polyvinyl alcohol 1788
Kind.
Since traditional immobilized cell form generally uses pellet form, exchanged inside immobilized spherule with substrate endless
Entirely, mass transfer is obstructed, and influences reaction rate；Investment is the common method of cell or enzyme immobilization, wherein the application of gel embedding method
Relatively broad, for polyvinyl alcohol as common used material a kind of in gel embedding, traditional application mode is molten using the boric acid of saturation
Liquid forms fine and close gel network as crosslinking agent.Structural formula is.Though fine and close gel network structure can guarantee immobilized cell
There is large effect with preferable elasticity and mechanical strength, but for substrate product mass transfer, become that restrict enzymatic anti-
The factor that should efficiently carry out.
The present invention uses polyvinyl alcohol as immobilization gel rubber material, and introduces polyethylene glycol as pore-foaming agent, is had
There is the immobilized cell of satisfactory gels network structure, reinforces its mass-transfer performance when guaranteeing its good mechanical strength.Gu
Surely the structural formula of change sequin is.The present invention uses PVA-
The composite materials immobilization propionic acid bar bacterium such as PEG, prepares the immobilization sequin of platypelloid type, keeps immobilization sequin existing higher
Mechanical strength, also compensate for the low disadvantage of immobilized spherule vigor compared with free cell.The process for fixation and form are used
In fixed propionic acid bar bacterium and for the bioconversion of nitrile compounds, there is very high industrial application value.
Method of the invention is small to microbial damage, immobilized cell high mechanical strength, long service life；Environmental pollution
It is small, separating-purifying is simple, product purity is high, be suitable for scale industrialized production.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.It should be appreciated that this hair
Bright implementation is not limited by the following examples, and the accommodation in any form made to the present invention and/or changed will all be fallen
Enter the scope of the present invention.
In the present invention, if not refering in particular to, all parts, percentage are unit of weight, used equipment and raw material etc.
It is commercially available or commonly used in the art.Method in following embodiments is unless otherwise instructed the normal of this field
Rule method.
Propionic acid bar bacterium is purchased from Shanghai Pesticide Research Institute Co., Ltd.
Embodiment 1
A kind of method that composite material immobilization propionic acid bar bacterium prepares niacinamide, this method step is specifically:
1) the present embodiment uses nitrile hydratase producing strains --- and for propionic acid bar bacterium thallus as immobilized thallus, propionic acid bar bacterium is logical
It crosses Liquid Culture and obtains bacterium solution, obtain wet thallus by centrifuge separation.
Fluid nutrient medium are as follows: glucose 20g, 10 g of yeast extract, 5 g of urea, 1 g of potassium dihydrogen phosphate, dipotassium hydrogen phosphate 1
G, 1 g of magnesium sulfate, 3.5 g of sodium glutamate, 0.003 g of cobalt chloride add water to 1000ml, adjust pH value to 7.0.
Condition of culture are as follows: from the slant strains picking propionic acid bar bacterium of eggplant bottle, be inoculated in Shake flask medium.250ml
The bottled 50ml culture medium of triangle, on shaking table with 200rpm constant-temperature shaking culture, 30 DEG C of cultivation temperature, incubation time 48h.
1000ml fermentation liquid is subjected to centrifugal treating, supernatant is removed, obtains the cell of wet nitrile hydratase production.By thallus
It is resuspended in deionized water solution, the cell suspension for the nitrile hydratase production that concentration is 50g/L is sufficiently stirred into.
2) immobilization of propionic acid bar bacterium:
(1) immobilization composite material:
Formula of the immobilization with composite material in terms of mass percentage are as follows:
Polyvinyl alcohol 1799(PVA 1799) 5%,
Polyethylene glycol 200 (PEG 200) 3%,
Diatomite 0.1%,
Active carbon 0.1%,
Surplus is water, and the above constituent mass adds up to 100%；
Polyvinyl alcohol, polyethylene glycol add after water Hybrid Heating stirring to dissolve it sufficiently to obtain PVA-PEG hydrogel, stirring rate
Control sequentially adds diatomite, is cooled to room temperature (25~30 after active carbon is sufficiently stirred within the scope of 300~500rpm
DEG C), obtain immobilization composite material；
(2) the to be fixedization thallus (wet thallus) obtained by Liquid Culture is mixed with immobilization with composite material, is sufficiently stirred
It mixes, is uniformly mixed thallus with hydrogel；The generation of bubble in order to prevent, stirring rate cannot be too fast, control 50~
Within the scope of 150rpm；
(3) uniformly mixed mixed liquor is picked using drop machine, the mixture that step (2) obtains is dripped on PE plate, is formed straight
The dot matrix of 3~6mm of diameter；
(4) the PE plate that the drop for obtaining step (3) has sequin is put into 30 DEG C of drying 60min in baking oven, is down to its moisture content
25%；
(5) the PE plate that step (4) obtains is placed in 5% Na2SO4In aqueous solution, make the sequin on PE plate in Na2SO4Aqueous solution
Middle submergence 20min；
(6) the PVA-PEG sequin of preparation is collected, clear water rinsing is placed in 2~10 DEG C of preservations；
3) preparation of niacinamide:
In 1000ml glass reaction kettle, 500g water is added, immobilization sequin 50g is added, opens and stirs and pass through collet temperature control
22 DEG C, continuous flow adds the nicotinonitrile of molten state to make the concentration 21.4% of final system, and stream adds into that rear the reaction was continued
The residual quantity of nicotinonitrile in 60min sample detection system, completely blowing after reaction.Immobilized cell is stayed to be relayed in reaction kettle
It is continuous to carry out next batch reaction.Using the residual quantity of HPLC detection nicotinonitrile and the production quantity of niacinamide.
Experimental result: hydrolysis obtains the nicotinamide soln of 25% concentration, and immobilized cell after the applying of 40 batches by remaining to
Conversion completely is realized, and immobilized cell itself still keeps good mechanical performance, without damaged Swelling.
Embodiment 2
A kind of method that composite material immobilization propionic acid bar bacterium prepares niacinamide, this method step is specifically:
1) the present embodiment uses nitrile hydratase producing strains --- propionic acid bar bacterium thallus as immobilized thallus,
Fluid nutrient medium are as follows: glucose 30g, 15 g of yeast extract, urea 7.5 g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5
G, 1.5 g of magnesium sulfate, 3.5 g of sodium glutamate, 0.005 g of cobalt chloride add water to 1000ml, adjust pH value to 6.8.
Condition of culture are as follows: from the slant strains picking propionic acid bar bacterium of eggplant bottle, be inoculated in Shake flask medium.250ml
The bottled 50ml culture medium of triangle, on shaking table with 240rpm constant-temperature shaking culture, 28 DEG C of cultivation temperature, incubation time 96h.
1000ml fermentation liquid is subjected to centrifugal treating, supernatant is removed, obtains the cell of wet nitrile hydratase production.By thallus
It is resuspended in deionized water solution, the cell suspension for the nitrile hydratase production that concentration is 75g/L is sufficiently stirred into.
2) immobilization of propionic acid bar bacterium:
(1) immobilization composite material:
Formula of the immobilization with composite material in terms of mass percentage are as follows:
Polyvinyl alcohol 1788(PVA 1788) 8%,
Polyethylene glycol 400 (PEG 400) 5%,
Diatomite 0.1%,
Active carbon 0.1%,
Surplus is water, and the above constituent mass adds up to 100%；
Polyvinyl alcohol, polyethylene glycol add after water Hybrid Heating stirring dissolve it sufficiently to obtain PVA-PEG hydrogel, then successively add
Enter diatomite, be cooled to room temperature after active carbon is sufficiently stirred, obtains immobilization composite material；
(2) the to be fixedization thallus obtained by Liquid Culture is mixed with immobilization with composite material, is sufficiently stirred, makes thallus
It is uniformly mixed with hydrogel；
(3) mixture that step (2) obtains is dripped on PE plate, forms the dot matrix of 3~6mm of diameter；
(4) the PE plate that the drop for obtaining step (3) has sequin is put into 28 DEG C of drying 30min in baking oven, is down to its moisture content
30%；
(5) the PE plate that step (4) obtains is placed in 8%Na2SO4In aqueous solution, make the sequin on PE plate in Na2SO4Aqueous solution
Middle submergence 30min；
(6) the PVA-PEG sequin of preparation is collected, clear water rinsing is placed in 2~10 DEG C of preservations；
3) preparation of niacinamide:
In 1000ml glass reaction kettle, 500g water is added, immobilization sequin 50g is added, opens and stirs and pass through collet temperature control
22 DEG C, continuous flow adds the nicotinonitrile of molten state to make the concentration 21.4% of final system, and stream adds into that rear the reaction was continued
The residual quantity of nicotinonitrile in 60min sample detection system, completely blowing after reaction.Immobilized cell is stayed to be relayed in reaction kettle
It is continuous to carry out next batch reaction.Using the residual quantity of HPLC detection nicotinonitrile and the production quantity of niacinamide.
Experimental result: hydrolysis obtains the nicotinamide soln of 25% concentration, and immobilized cell after the applying of 45 batches by remaining to
Conversion completely is realized, and immobilized cell itself still keeps good mechanical performance, without damaged Swelling.
Embodiment 3
A kind of method that composite material immobilization propionic acid bar bacterium prepares niacinamide, this method step is specifically:
1) the present embodiment uses nitrile hydratase producing strains --- propionic acid bar bacterium thallus as immobilized thallus,
Fluid nutrient medium are as follows: glucose 25g, 7.5 g of yeast extract, 3.5 g of urea, 0.75 g of potassium dihydrogen phosphate, dipotassium hydrogen phosphate
0.75 g, 0.75 g of magnesium sulfate, 3 g of sodium glutamate, 0.002 g of cobalt chloride add water to 1000ml, adjust pH value to 7.2.
Condition of culture are as follows: from the slant strains picking propionic acid bar bacterium of eggplant bottle, be inoculated in Shake flask medium.250ml
The bottled 50ml culture medium of triangle, on shaking table with 220rpm constant-temperature shaking culture, 30 DEG C of cultivation temperature, incubation time 72h.
1000ml fermentation liquid is subjected to centrifugal treating, supernatant is removed, obtains the cell of wet nitrile hydratase production.By thallus
It is resuspended in deionized water solution, the cell suspension for the nitrile hydratase production that concentration is 50g/L is sufficiently stirred into.
2) immobilization of propionic acid bar bacterium:
(1) immobilization composite material:
Formula of the immobilization with composite material in terms of mass percentage are as follows:
Polyvinyl alcohol 1788(PVA 1788) 10%,
Cetomacrogol 1000 (PEG 1000) 8%,
Diatomite 0.1%,
Active carbon 0.1%,
Surplus is water, and the above constituent mass adds up to 100%；
Polyvinyl alcohol, polyethylene glycol add after water Hybrid Heating stirring dissolve it sufficiently to obtain PVA-PEG hydrogel, then successively add
Enter diatomite, be cooled to room temperature after active carbon is sufficiently stirred, obtains immobilization composite material；
(2) the to be fixedization thallus obtained by Liquid Culture is mixed with immobilization with composite material, is sufficiently stirred, makes thallus
It is uniformly mixed with hydrogel；
(3) mixture that step (2) obtains is dripped on PE plate, forms the dot matrix of 3~6mm of diameter；
(4) the PE plate that the drop for obtaining step (3) has sequin is put into 25 DEG C of drying 120min in baking oven, is down to its moisture content
20%；
(5) the PE plate that step (4) obtains is placed in 10%Na2SO4In aqueous solution, make the sequin on PE plate in Na2SO4Aqueous solution
Middle submergence 40min；
(6) the PVA-PEG sequin of preparation is collected, clear water rinsing is placed in 2~10 DEG C of preservations；
3) preparation of niacinamide:
In 1000ml glass reaction kettle, 500g water is added, immobilization sequin 50g is added, opens and stirs and pass through collet temperature control
22 DEG C, continuous flow adds the nicotinonitrile of molten state to make the concentration 21.4% of final system, and stream adds into that rear the reaction was continued
The residual quantity of nicotinonitrile in 60min sample detection system, completely blowing after reaction.Immobilized cell is stayed to be relayed in reaction kettle
It is continuous to carry out next batch reaction.Using the residual quantity of HPLC detection nicotinonitrile and the production quantity of niacinamide.
Experimental result: hydrolysis obtains the nicotinamide soln of 25% concentration, and immobilized cell after the applying of 60 batches by remaining to
Conversion completely is realized, and immobilized cell itself still keeps good mechanical performance, without damaged Swelling.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form
Limitation, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.
Claims (7)
1. a kind of method that composite material immobilization propionic acid bar bacterium prepares niacinamide, it is characterised in that this method includes following step
It is rapid:
1) immobilization of propionic acid bar bacterium:
(1) immobilization composite material:
Formula of the immobilization with composite material in terms of mass percentage are as follows:
Polyvinyl alcohol (PVA) 5%~20%,
Polyethylene glycol (PEG) 3%~10%,
Diatomite 0.1%~2%,
Active carbon 0.1%~1%,
Surplus is water, and the above constituent mass adds up to 100%；
Polyvinyl alcohol, polyethylene glycol add after water Hybrid Heating stirring dissolve it sufficiently to obtain PVA-PEG hydrogel, then successively add
Enter diatomite, be cooled to room temperature after active carbon is sufficiently stirred, obtains immobilization composite material；
(2) the to be fixedization thallus obtained by Liquid Culture is mixed with immobilization with composite material, is sufficiently stirred, makes thallus
It is uniformly mixed with immobilization with composite material；
(3) mixture that step (2) obtains is dripped on PE plate, forms the dot matrix of 3~6mm of diameter；
(4) drop for obtaining step (3) has the PE plate of sequin to be put into baking oven, its moisture content is made to be down to 10%~25%；
(5) the PE plate that step (4) obtains is placed in Na2SO4In aqueous solution, make the sequin on PE plate in Na2SO4It is soaked in aqueous solution
Not yet；
(6) the PVA-PEG sequin of preparation, cryo-conservation after cleaning are collected；
2) preparation of niacinamide:
The immobilization sequin that above-mentioned steps obtain is added in the nicotinonitrile solution that mass concentration is 10~30%, it is fixed
The additional amount for changing sequin is 10-30%, obtains niacinamide after 60~240min of reaction time.
2. the method that composite material immobilization propionic acid bar bacterium according to claim 1 prepares niacinamide, feature exist
In: in step (2), the fluid nutrient medium of thallus is formed are as follows: 10~40g of glucose, 5~20g of yeast extract, 2~10g of urea, phosphorus
Acid dihydride 1~5g of potassium, 1~5g of dipotassium hydrogen phosphate, 1~5g of magnesium sulfate, 2~6g of sodium glutamate, 0.001~0.01g of cobalt chloride add
For water to 1L, pH value is 6.8~7.5；Condition of culture are as follows: the culture medium of 250ml triangle bottled 10~30%, after sterilizing be added 1~
10% strain, on shaking table with 100~300rpm constant-temperature shaking culture, 25~35 DEG C of cultivation temperature, incubation time 36~
108h。
3. the method that composite material immobilization propionic acid bar bacterium according to claim 1 prepares niacinamide, it is characterised in that:
In step (2), the mass ratio of thallus and immobilization composite material is 0.1~1:10.
4. the method that composite material immobilization propionic acid bar bacterium according to claim 1 prepares niacinamide, it is characterised in that:
The oven temperature setting for declining PVA-PEG sequin moisture content in step (4) is at 20-50 DEG C, drying time 30-
120min。
5. the method that composite material immobilization propionic acid bar bacterium according to claim 1 prepares niacinamide, it is characterised in that:
In step (5), Na2SO4Mass percentage be 1~20%, the soaking time of sequin is 20~40min.
6. the method that composite material immobilization propionic acid bar bacterium according to claim 1 prepares niacinamide, it is characterised in that:
Storage temperature is 2~10 DEG C in step (6).
7. the method that composite material immobilization propionic acid bar bacterium according to claim 1 prepares niacinamide, it is characterised in that:
The polyethylene glycol is polyethylene glycol 200, polyethylene glycol 400, polyethylene glycol-800, polyethylene glycol 2000, Macrogol 4000
In any one；The polyvinyl alcohol is one of polyvinyl alcohol 1799, polyvinyl alcohol 1788.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114317506A (en) * | 2022-01-13 | 2022-04-12 | 兄弟科技股份有限公司 | Nitrilase, engineering bacteria constructed by nitrilase and application of nitrilase in green synthesis of nicotinic acid |
| CN114480361A (en) * | 2021-12-27 | 2022-05-13 | 安徽泰格生物科技有限公司 | Immobilized bacteria and preparation method of nicotinamide |
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| CN114480361B (en) * | 2021-12-27 | 2024-05-10 | 安徽泰格生物科技有限公司 | Preparation method of immobilized thalli and nicotinamide |
| CN114317506A (en) * | 2022-01-13 | 2022-04-12 | 兄弟科技股份有限公司 | Nitrilase, engineering bacteria constructed by nitrilase and application of nitrilase in green synthesis of nicotinic acid |
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