CN110124086B - Composite nanofiber pad, hydrogel/sponge dressing, preparation method and application - Google Patents
Composite nanofiber pad, hydrogel/sponge dressing, preparation method and application Download PDFInfo
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- CN110124086B CN110124086B CN201910438702.0A CN201910438702A CN110124086B CN 110124086 B CN110124086 B CN 110124086B CN 201910438702 A CN201910438702 A CN 201910438702A CN 110124086 B CN110124086 B CN 110124086B
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- hydrogel
- composite nanofiber
- nanofiber mat
- dressing
- matrix
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- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
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Abstract
The invention discloses a composite nanofiber mat, a hydrogel/sponge dressing, a preparation method and application, and relates to the technical field of medical materials. The utility model provides a compound nanofiber mat, includes the receiving plate, and the receiving plate is last to be compounded and to have acellular matrix and macromolecular material, and the matrix that goes the cell is compared with macromolecular material's interpolation: 1: 1-1: 6, and the composite nanofiber mat is of a micro-nanofiber structure. The hydrogel/sponge dressing provided by the invention can be used for loading components such as nutritional factors and medicines for promoting wound regeneration and repair, and has good biocompatibility and no obvious cytotoxicity; the hydrogel/sponge dressing material is soft, can tightly cover all wound surfaces, resists bacterial invasion and avoids wound surface infection.
Description
Technical Field
The invention relates to the technical field of medical materials, in particular to a composite nanofiber mat, a hydrogel/sponge dressing, a preparation method and application.
Background
The wound refers to the place where the wound is broken, and is mostly referred to as skin, muscle, mucous membrane, etc. of a human or other animal. Wounds can generally be divided into acute and chronic difficult-to-heal wounds, and wound healing can generally be divided into four distinct but overlapping stages, coagulation/hemostasis, inflammatory response/migration, proliferation/vascularization/regeneration, and tissue remodeling, respectively. Normally, a wound needs about three weeks to heal, but chronic difficult-to-heal wounds are susceptible to bacterial infection, so that the time for wound healing is prolonged. Human wounds are exposed to easily cause bacterial infection, and meanwhile, in order to meet the requirements of local medicine application, moisture retention, drainage liquid absorption and the like, the wound dressing is required to be used for improving the local chemical and physical environment of the wounds to promote the healing of the wounds.
The acellular matrix is a material which retains extracellular matrix components of natural tissues, and the material has the high possibility of causing problems of immunogenicity, disease infection and the like after being transplanted into a body. The decellularization technology destroys cells by physical or chemical methods, separates the cells from the extracellular matrix of tissues, and removes DNA/RNA which can cause immune reaction and infectious diseases to obtain a pure extracellular matrix material.
An ideal wound dressing should have the following characteristics, namely 1. prevent wound reinfection; 2. protecting the wound from re-wounding; 3. providing a medicament; 4. keeping a certain humidity; 5. has good permeability; 6. absorbing the drainage and cleaning the wound; 7. has certain mechanical strength; 8. the skin is not damaged when the skin is removed. The current medical wound dressings comprise natural gauze, synthetic fiber dressings, polymeric film dressings, foaming polymeric dressings, hydrocolloid dressings, alginate dressings and the like.
Traditional wound dressings such as gauze dressings and synthetic fiber dressings have too high permeability, easily enable the wound surface to dehydrate, easily allow external environment microorganisms to pass through, have high chance of cross infection, are easy to adhere to the wound surface, and can cause secondary mechanical injury during replacement.
Other wound dressings prepared from the novel synthetic material, such as polymeric film dressings, have poor capability of absorbing seepage and large chance of skin impregnation around the wound surface; the foamed polymer dressing has too strong absorption performance, so that the wound surface with low exudation can be affected in the self-debridement process, and the wound surface is not convenient to observe because of the opacity; hydrocolloid dressings are not very absorbent and therefore for high exudation wounds other auxiliary dressings are often required to enhance the absorption properties and individual patients may be allergic to the ingredients. Alginate dressings most products are not self-adhesive and require auxiliary dressings to be affixed.
Compared with the materials, the acellular matrix has similar components and structures with natural tissues, but the acellular matrix material cannot be independently used as a dressing due to lower mechanical strength.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a composite nanofiber mat which has a compact structure of micro-nanofibers and can play a role in isolating external bacterial infection.
Another objective of the present invention is to provide a method for preparing a composite nanofiber mat, which provides a mechanical support for the matrix nanofibers without cells, overcomes the weak strength of the matrix nanofibers without cells, and simultaneously maintains the internal micro-nano bionic structure, thereby facilitating the adhesion, proliferation and migration of cells and providing a good carrier for drugs.
The invention also provides a preparation method of the hydrogel/sponge dressing, the upper layer of the dressing prepared by the method is the composite nanofiber mat, the lower layer is acellular matrix hydrogel or sponge, a porous loose structure with a micro-nano structure exists in the dressing, the upper layer and the lower layer of the dressing can repair the epidermis layer and the dermis layer of the damaged skin at the same time, and meanwhile, as the upper layer and the lower layer of the dressing both contain the acellular matrix, more biological components are reserved, and the dressing is more beneficial to cell adhesion, proliferation and migration than the traditional dressing.
The invention is realized by the following steps:
a composite nanofiber mat is compounded with a decellularized matrix and a high polymer material, wherein the addition ratio of the decellularized matrix to the high polymer material is as follows: 1: 1-1: 6, the composite nanofiber mat is of a micro-nanofiber structure, and preferably, the addition ratio of the acellular matrix to the high polymer material is as follows: 1:6.
In a preferred embodiment of the present invention, the material source of the decellularized matrix can be any one of pig, cow and human, and the decellularized matrix can be any one or more of skin extracellular matrix, small intestine submucosa extracellular matrix and amniotic membrane extracellular matrix.
In a preferred embodiment of the present invention, the polymer material may be any one of polycaprolactone, polylactic acid, levorotatory polylactic acid, hyaluronic acid and gelatin.
A preparation method of a composite nanofiber mat comprises the steps of respectively preparing an acellular matrix and an electrospinning solution of a high polymer material, transferring the electrospinning solution of the acellular matrix into a first injector, transferring the electrospinning solution of the high polymer material into a second injector, and co-spinning or blending the acellular matrix and the electrospinning solution of the high polymer material on a receiving plate by adopting an electrospinning method to prepare the composite nanofiber mat.
In a preferred embodiment of the present invention, the injection speed of the first injector is 1 mL/h-4 mL/h, the injection speed of the second injector is 1 mL/h-4 mL/h, the needles of the first injector and the second injector are both international standard 8-9 double needles, the coaxial needle and the Y-type international standard 9 needle are any one, and the distance between the needle and the receiving plate is 6-10 cm;
preferably, the injection speed of the first injector is 3mL/h, the injection speed of the second injector is 3mL/h, the needles of the first injector and the second injector are Y-type international standard No. 9 needles, and the distance between the needle and the receiving plate is 7 cm.
In the preferred embodiment of the invention, the positive voltage applied to the needle is 8-25kV, and the negative voltage applied to the receiving plate is 0.5-1.0 kV;
preferably, the positive voltage applied to the needle is 13kV, and the negative voltage applied to the receiving plate is 1.0 kV.
In a preferred embodiment of the present invention, the receiving plate is made of an electrically conductive material, and preferably, the receiving plate is a stainless steel plate encapsulated by tinfoil.
A hydrogel/sponge dressing comprising a composite nanofiber pad and an acellular matrix hydrogel/sponge, the composite nanofiber pad underlying the acellular matrix hydrogel/sponge.
A process for preparing the hydrogel/sponge dressing includes such steps as digesting the matrix without cells with digestive liquid, centrifugal separation, regulating pH value to obtain cracking solution, coating the cracking solution on the composite nano-fibre pad, and thermal insulating.
In a preferred embodiment of the present invention, the digestive juice is one or a combination of pepsin, trypsin and papain, preferably, the digestive juice is pepsin, and the pH adjusting step comprises adjusting the pH to be higher than 8 with NaOH, and adjusting the pH to be 7-7.5 with HCl to obtain a lysis solution; the heat preservation step comprises the steps of placing the composite nanofiber mat coated with the pyrolysis solution in a heat preservation box at 35-40 ℃ for standing for 2-10 minutes to prepare a hydrogel dressing, and carrying out vacuum freeze-drying on the composite nanofiber mat coated with the pyrolysis solution to prepare a sponge dressing; vacuum freeze-drying conditions were-50 deg.C, 0.1 Pa.
In the preferred embodiment of the invention, 10% pepsin hydrochloric acid digestive juice is used for digesting the acellular matrix for 12-48 hours, the obtained enzymolysis liquid is placed in a high-speed centrifuge for high-speed centrifugation for 45 minutes at 30000rpm and 4 ℃, the pH value is adjusted to be more than 8 by NaOH, and the pH value is adjusted to be 7-7.5 by HCl, so as to obtain a pre-gel solution; and adding 10xPBS into the neutral pre-gel solution at 4 ℃, coating the pre-gel solution in a composite nano fiber pad, and standing the composite nano fiber pad in a 37 ℃ incubator for 2-10 minutes to obtain the hydrogel dressing.
In the embodiment of the invention with better application, 10% pepsin hydrochloric acid digestive juice is used for digesting the acellular matrix for 12-48 hours, the obtained lysate is placed in a high-speed centrifuge for high-speed centrifugation for 45 minutes and 2 times at 30000rpm and 4 ℃, the pH value is adjusted to be 7 by NaOH, the obtained enzymolysis liquid is coated in a composite nanofiber mat, and vacuum freeze-drying is carried out at-50 ℃ and 0.1Pa, so as to obtain the sponge dressing.
In the preferred embodiment of the invention, 10% pepsin hydrochloric acid digestive juice is used to digest the acellular matrix for 12-48 hours, the obtained lysate is placed in a high-speed centrifuge to be centrifuged at a high speed of 30000rpm and 4 ℃ for 45 minutes, the pH value is adjusted to be more than 8 by NaOH, and the pH value is adjusted to be 7-7.5 by HCl, so as to obtain a pre-gel solution; adding 10xPBS into the neutral pregel solution at 4 ℃, coating the pregel solution on a composite nano fiber pad, standing the composite nano fiber pad in a 37 ℃ incubator for 2-10 minutes to form gel, and performing vacuum freeze-drying on the gel in a vacuum freeze-drying machine at-50 ℃ and 0.1Pa to obtain the sponge dressing.
Use of a composite nanofiber mat in the preparation of a hydrogel/sponge dressing.
The invention has the following beneficial effects:
the invention provides a composite nanofiber mat, which has a compact structure of micro-nanofibers and can play a role in isolating external bacterial infection, and the strength of matrix nanofibers subjected to cell removal is improved by adding a high polymer material into the composite nanofiber mat.
The invention also provides a preparation method of the composite nanofiber mat, which plays a mechanical supporting role for the matrix nanofibers subjected to cell removal, overcomes the problem of weak strength of the matrix nanofibers subjected to cell removal only, can keep the internal micro-nano bionic structure, is beneficial to cell adhesion, proliferation and migration, and provides a good carrier for medicaments.
The invention also provides a preparation method of the hydrogel/sponge dressing, the upper layer of the dressing prepared by the method is the composite nanofiber mat, the lower layer is acellular matrix hydrogel or sponge, a porous loose structure with a micro-nano structure exists in the dressing, the upper layer and the lower layer of the dressing can repair the epidermis layer and the dermis layer of the damaged skin at the same time, and meanwhile, as the upper layer and the lower layer of the dressing both contain the acellular matrix, more biological components are reserved, and the dressing is more beneficial to cell adhesion, proliferation and migration than the traditional dressing.
Use of a composite nanofiber mat in the preparation of a hydrogel/sponge dressing.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a scanning electron micrograph of a decellularized matrix nanofiber mat as provided in example 1 of the present invention;
FIG. 2 is a scanning electron micrograph of the side of a decellularized matrix hydrogel dressing provided in example 2 of the present invention;
FIG. 3 is a scanning electron microscope image of the interface of the upper hydrogel and the lower nanofiber mat of the acellular matrix hydrogel dressing provided in example 2 of the present invention;
FIG. 4 is a scanning electron micrograph of an overlying hydrogel of a decellularized matrix hydrogel dressing provided in example 2 of the present invention;
FIG. 5 is a scanning electron micrograph of the side of a decellularized matrix sponge dressing provided in example 3 of the present invention;
FIG. 6 is a scanning electron microscope image of the interface of the upper sponge and the lower nanofiber mat of the acellular matrix sponge dressing provided in example 3 of the present invention;
FIG. 7 is a scanning electron micrograph of the upper sponge of a decellularized matrix sponge dressing provided in example 3 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
Preparation of composite nanofiber mats
(1) A decellularized matrix is prepared. Collecting pig skin tissue 20m3Cutting into small pieces, and washing with warm waterSoaking with 3% triton X-100 for 12 hr, washing with deionized water for 15 min and 3 times, treating with 4% sodium deoxycholate solution for 24 hr, washing with deionized water for 15 min and 3 times, vacuum lyophilizing for 2 days at-50 deg.C under 0.1 MPa. And (2) degreasing the vacuum freeze-dried matrix for 2 times by taking ethanol/dichloromethane (the volume ratio is 2: 1) which is 10 times of the volume of the acellular tissue and organ matrix, washing the vacuum freeze-dried matrix for 3 times by using deionized water for 1 day each time, carrying out vacuum filtration overnight for 15 minutes each time, carrying out vacuum freeze-drying for 2 days, pulverizing, sieving by using a 40-mesh sieve, and storing at-40 ℃ to obtain acellular matrix powder.
The source of the acellular matrix material can be any one of pig, cattle and human, and the acellular matrix can be any one or combination of skin extracellular matrix, small intestine submucosa extracellular matrix and amniotic extracellular matrix. In the present invention, other extracellular matrices are similarly effective as main materials.
(2) An electrospinning solution was prepared. Dissolving 0.25g of the cell-matrix-removed powder obtained in the step (1) in 10ml of hexafluoroisopropanol, stirring for 4-6 days, dividing into 5 parts after stirring, adding into 2ml of EP tubes, adding 2 steel balls or zirconia balls with the diameter of 5mm and the diameter of 1mm into each EP tube, placing 5 EP tubes in a ball mill with the experimental conditions of-10 ℃ and 60Hz, carrying out ball milling for 10 minutes and 2 times, and transferring into an injector 1 after the ball milling is finished; taking 1.5g of polycaprolactone, dissolving the polycaprolactone in 10ml of hexafluoroisopropanol or trifluoroethanol, stirring for 1 day, and transferring the polycaprolactone into a syringe 2 after stirring.
(3) Preparing the matrix/high polymer material composite nanofiber mat with the upper layer being decellularized. The conditions for preparing the acellular matrix/high polymer material composite nanofiber mat by electrospinning are as follows: the injection speed of the injector 1 is 3mL/h, the injection speed of the injector 2 is 3mL/h, the injectors 1 and 2 both adopt Y-type international standard No. 9 needles, the distance between the needle and a receiving flat plate is 7cm, the receiving plate is a stainless steel plate encapsulated by tinfoil, the needles of the injectors 1 and 2 are applied with positive voltage of 13kV, and the receiving flat plate is applied with negative voltage of 1.0 kV.
Example 2
Preparation of acellular matrix full-thickness skin repair hydrogel dressing
(1) Weighing 0.01g of pepsin, mixing the pepsin with 10ml of pepsin, stirring the pepsin in a 0.01% hydrochloric acid solution for 30 minutes to prepare a stirring solution, adding 0.1g of the acellular matrix powder in the embodiment 1 into the stirring solution after stirring to prepare a digestion solution, continuously stirring and digesting the digestion solution for 24 hours, adding the obtained enzymolysis solution into a centrifugal tube, placing the centrifugal tube into a high-speed centrifuge, centrifuging the centrifugation solution for 2 times at a high speed for 45 minutes at a temperature of 4 ℃ at a speed of 30000rpm, taking supernate, adjusting the pH value of the enzymolysis solution to be more than 8 by using 0.1% NaOH, and adjusting the pH value to be 7-7.5 by using HCl to obtain a pre-gel solution;
(2) 10xPBS was added to the neutral pregel solution at 4 ℃ and coated on the composite nanofiber mat of example 1, which was left to stand in an incubator at 37 ℃ for 10 minutes to gel, to obtain a acellular matrix full-thickness skin repair hydrogel dressing.
Example 3
Preparation of acellular matrix full-thickness skin repair sponge dressing
Weighing 0.01g of pepsin, mixing the pepsin into 10ml of 0.01% hydrochloric acid solution, stirring for 30 minutes, adding 0.1g of the acellular matrix powder in the example 1 after stirring to prepare a digestive juice, continuing to stir and digest for 24 hours, adding the obtained lysate into a centrifuge tube, placing the centrifuge tube into a high-speed centrifuge, centrifuging at 30000rpm and 4 ℃ for 45 minutes at a high speed, centrifuging for 2 times, adjusting the pH value of the lysate to 7 by using 0.1% NaOH, coating the obtained lysate in the composite nanofiber mat in the example 1, and performing vacuum freeze-drying at-50 ℃ and 0.1Pa for 3 days to obtain the acellular matrix full-layer skin repair sponge dressing.
In other embodiments of the present invention, the acellular matrix full-thickness skin repair sponge dressing can also be prepared by the following method:
0.01g of pepsin was weighed, mixed with 10ml of a 0.01% hydrochloric acid solution, and stirred for 30 minutes, and 0.1g of the decellularized matrix powder of example 1 was added after completion of stirring, to prepare a digestion solution. Continuously stirring and digesting for 24 hours, adding the obtained lysate into a centrifugal tube, placing the centrifugal tube into a high-speed centrifuge, centrifuging for 2 times at a high speed for 45 minutes at the temperature of 4 ℃ at 30000rpm, removing supernatant, adjusting the pH value of the enzymolysis solution to be more than 8 by using 0.1% NaOH, and adjusting the pH value to be 7-7.5 by using HCl to obtain a pre-gel solution; adding 10xPBS into the neutral pregel solution at 4 ℃, coating the pregel solution in the composite nano fiber pad in the example 1, standing the pregel solution in a 37 ℃ incubator for 10 minutes to gelatinize, and after the gelatinizing, putting the pregel solution in a vacuum freeze dryer at-50 ℃ and 0.1Pa for vacuum freeze-drying for 3 days to obtain the acellular matrix full-layer skin repair sponge dressing.
Example 4
The structures of the materials obtained in example 1, example 2 and example 3 were observed under a scanning electron microscope (Hitachi Tech, S-4800).
An enlarged scanning electron microscope image of the nanofiber mat obtained in example 1 is shown in fig. 1.
The scanning electron microscope image of the side surface of the acellular matrix hydrogel dressing obtained in example 2 is shown in fig. 2, the scanning electron microscope image of the interface between the upper hydrogel layer and the lower nanofiber mat of the acellular matrix hydrogel dressing is shown in fig. 3, and the scanning electron microscope image of the upper hydrogel layer is shown in fig. 4. Example 3 a scanning electron microscope image of the side of the acellular matrix sponge dressing is shown in fig. 5, a scanning electron microscope image of the interface between the upper sponge and the lower nanofiber mat of the acellular matrix sponge dressing is shown in fig. 6, and a scanning electron microscope image of the upper sponge of the sponge dressing is shown in fig. 7.
As can be seen from fig. 1, the composite nanofiber mat in example 1 has a small fiber diameter, compact fibers, and a micro-nano structure, and is beneficial to isolating external bacteria from invading and internal cells from adhering and differentiating.
As can be seen from fig. 2 to 4, the acellular matrix hydrogel dressing prepared in example 2 has the characteristics of micro-nano multilevel fibers, the fiber pore size is large, cell attachment, differentiation and migration are facilitated, the lower layer is a composite nanofiber mat, and the upper layer is hydrogel.
As can be seen from fig. 5 to 7, the acellular matrix sponge dressing prepared in example 3 has the characteristics of micro-nano hierarchical porous shape, the pore size of the sponge is large, cell attachment, differentiation and migration are facilitated, the lower layer is the composite nanofiber mat, and the upper layer is the sponge.
According to the composite nanofiber mat, the effect of external bacterial infection is isolated, and the strength of the matrix nanofiber without cells is improved by adding the high polymer material into the composite nanofiber mat. According to the preparation method of the composite nanofiber mat, the preparation method plays a mechanical supporting role for the matrix nanofiber subjected to cell removal, the problem that the matrix nanofiber subjected to cell removal is weak in strength is solved, the internal micro-nano bionic structure can be maintained, cell adhesion, proliferation and migration are facilitated, and a good carrier is provided for drugs.
The invention also provides a preparation method of the hydrogel/sponge dressing, the upper layer of the dressing prepared by the method is the composite nanofiber mat, the lower layer is acellular matrix hydrogel or sponge, a porous loose structure with a micro-nano structure exists in the dressing, the upper layer and the lower layer of the dressing can repair the epidermis layer and the dermis layer of the damaged skin at the same time, and meanwhile, as the upper layer and the lower layer of the dressing both contain the acellular matrix, more biological components are reserved, and the dressing is more beneficial to cell adhesion, proliferation and migration than the traditional dressing.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A preparation method of a composite nanofiber mat is characterized by respectively preparing an acellular matrix and an electrospinning solution of a high polymer material, transferring the acellular matrix electrospinning solution into a first injector, transferring the high polymer material electrospinning solution into a second injector, and co-spinning or blending the acellular matrix and the high polymer material electrospinning solution on a receiving plate by adopting an electrospinning method to prepare the composite nanofiber mat;
the adding ratio of the acellular matrix to the high polymer material is 1: 6; the injection speed of the first injector is 3mL/h, and the injection speed of the second injector is 3 mL/h;
the high polymer material can be any one of polycaprolactone, polylactic acid and levorotatory polylactic acid.
2. The method for preparing a composite nanofiber mat as claimed in claim 1, wherein the needles of the first syringe and the second syringe are any one of international standard 8-9 gauge double needles, coaxial needles and Y-type international standard 9 gauge needles, and the distance between the needle and the receiving plate is 6-10 cm.
3. The method for preparing a composite nanofiber mat according to claim 2, wherein the needles of the first syringe and the second syringe are Y-type International Standard 9 needles, and the distance between the needle and the receiving plate is 7 cm.
4. The method of claim 2, wherein the positive voltage applied to the needle is 13kV and the negative voltage applied to the receiving plate is 1.0 kV.
5. The composite nanofiber mat prepared by the preparation method of any one of claims 1 to 4 is characterized by being compounded with a decellularized matrix and a high polymer material, wherein the addition ratio of the decellularized matrix to the high polymer material is 1:6, and the composite nanofiber mat is in a micro-nanofiber structure.
6. A preparation method of the hydrogel/sponge dressing is characterized in that a acellular matrix is digested by digestive juice, centrifuged and adjusted in pH to prepare a lysis solution, the lysis solution is coated in the composite nanofiber mat of claim 5, and the hydrogel/sponge dressing is prepared by heat preservation.
7. The preparation method of the hydrogel/sponge dressing as claimed in claim 6, wherein the digestive juice is any one or combination of pepsin, trypsin and papain, and the pH adjusting step comprises firstly adjusting the pH value to be higher than 8 with NaOH, and then adjusting the pH value to be 7-7.5 with HCl to prepare a lysis solution; the heat preservation step comprises the step of placing the composite nanofiber mat coated with the pyrolysis solution in a heat preservation box at the temperature of 35-40 ℃ for standing for 2-10 minutes to prepare the hydrogel dressing; and carrying out vacuum freeze-drying on the composite nanofiber mat coated with the lysis solution to prepare the sponge dressing, wherein the vacuum freeze-drying condition is-50 ℃ and 0.1 Pa.
8. A hydrogel/sponge dressing comprising the composite nanofiber mat of claim 5 and an acellular matrix hydrogel/sponge, wherein the composite nanofiber mat is disposed beneath the acellular matrix hydrogel/sponge.
9. Use of the composite nanofiber mat as claimed in claim 5 in the preparation of a hydrogel/sponge dressing.
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