CN110117634A - A kind of five-cereal health-care peptide and preparation method thereof - Google Patents
A kind of five-cereal health-care peptide and preparation method thereof Download PDFInfo
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- CN110117634A CN110117634A CN201910402182.8A CN201910402182A CN110117634A CN 110117634 A CN110117634 A CN 110117634A CN 201910402182 A CN201910402182 A CN 201910402182A CN 110117634 A CN110117634 A CN 110117634A
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Abstract
The present invention relates to food processing fields, in particular to a kind of five-cereal health-care peptide and preparation method thereof, by various plants albumen, the chelated iron after multienzyme stepwise discretization is made five-cereal health-care peptide, and various plants albumen content meter by weight includes: 40-50 parts of soybean protein, 10-20 parts of wheat germ protein, 10-20 parts of zein, 5-10 parts of peanut protein, 5-10 parts of walnut protein；Various plants albumen passes through multi-enzymatic hydrolysis method, five cereals polypeptide is made, polypeptide molecular weight is between 500-1000Da, polypeptide chelate chalybeate makes chalybeate be converted into easy absorption, the Organic Iron easily stored, the separation of alcohol precipitation realization Organic Iron and inorganic iron, make the more preferable chelated iron of polypeptide, five cereals peptide can play idiotrophic value, while have the effect of blood-nourishing and iron-replenishing.
Description
Technical field
The present invention relates to food processing field, in particular to a kind of five-cereal health-care peptide and preparation method thereof.
Background technique
Some metallic elements especially microelement, such as iron, zinc, copper, selenium, although extremely low in people's in-vivo content,
Extreme influence the existence and health of people.Their insufficiency of intakes excessively can all cause Human Physiology disorder or disease to some extent
Disease.The research of polypeptide-chelate of metal ion starts from the last century 60's.The beginning of the eighties, U.S.'s Albion laboratory iron support
Elements compounding animal/vegetable protein generates albumen iron complexes.Thus start, the research of polypeptide-chelate of metal ion causes
The concern of whole world scholar.Currently, in the world there is the disease of the metallic elements such as calcium, iron, zinc shortage in the people in many areas
Shape, therefore, development of metallic component extender have great and real meaning.
Iron is minerals needed by human, and the iron in diet is mainly with Fe3+In the presence of body can not be utilized directly, Yi Yinqi
Asiderosis.Global about 3,000,000,000 people suffer from anaemia, and male's disease incidence is 10%, and women 20%, and pregnant woman 40%, and children are up to
50%.The defects of iron supplementary used at present is low in the presence of being absorbed and utilized, and stability is poor, stimulating gastrointestinal road.Therefore utilization rate is sought
Height, the ferrous-fortifier of Small side effects are the targets of numerous researchers.Amino-acid chelate is as third generation iron supplementary, utilization rate
It is universal higher.Ion channel need not be walked after metal ion and peptide or chelating amino acids, peptide can be entered or the channel of amino acid is inhaled
It receives, largely promotes the absorption of iron.Small peptide can completely be absorbed by organisms and enter blood in the form of two, tripeptides and follow
Ring.Peptide and free amino acid have relatively independent mechanism of absorption in human body, and compared with the transport system of amino acid, peptide exists
Absorption in human body, which has, to be absorbed fastly, and high-efficient, low energy consumption, and carrier is not easy the advantage being saturated, so peptide chelate complex is easier quilt
Body absorbs.
Summary of the invention
The present invention is in view of the above problems, provide a kind of five-cereal health-care peptide.
It is another object of the present invention to provide a kind of five-cereal health-care peptide preparation methods.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of five-cereal health-care peptide, by various plants albumen, the chelated iron after multienzyme stepwise discretization is made the five-cereal health-care peptide, institute
Stating various plants albumen content meter by weight includes: 40-50 parts of soybean protein, 10-20 parts of wheat germ protein, zein 10-
20 parts, 5-10 parts of peanut protein, 5-10 parts of walnut protein.
Soybean protein is a kind of vegetable protein.Soy proteinaceous amino acid composition is close with milk protein,
Except for slightly lower methionine, remaining essential amino acids content is relatively abundant, is vegetal complete protein, on nutritive value,
It can be equivalent to animal protein, human amino acid is also closest on gene structure, so being the phytoprotein of most nutrition.
Wheat embryo is a kind of high protein high microsteping, homovitamin E, low-heat, low fat, the full nutrition food of low cholesterol
Product, and vitamin E rich in, vitamin B complex, phytoprotein, unsaturated fatty acid, and a large amount of food fiber and
Minerals (calcium, iron, zinc, selenium etc.) abundant.
Corn protein powder is one of the main by-product in corn wet processing, can be divided into albumin according to its dissolubility
(Albumins, soluble easily in water), globulin (Globulins is soluble in salting liquid), zein (Zein, Prolamine, it is molten
In the alcoholic solution of higher concentration), glutelin (Glutelin is soluble in dilute alkaline soln).The physicochemical property of zein is derived from it
Special structure and amino acid classes and content.Zein is especially enriched in glutamic acid (21-26%), leucine (20%), dried meat ammonia
Sour (10%) and alanine (10%), the content highest of zein Glutamic Acid, this is the general character of grain protein.
Peanut protein completely remains the nutrition in peanut without protein heat denaturation, the higher vegetable protein of nutritive value
Ingredient, soluble protein and NSI value are high, and good water solubility is pure white, and flavor is light, have the distinctive faint scent of peanut, battalion
Feeding value can be comparable with animal protein, is rich in a large amount of essential amino acid, vitamin, microelement and minerals, has
Utilization rate is imitated up to 98%, and easily digest and assimilate and contain anti-nutritional factors more less than soybean for human body, is low sugar, low fat,
It is cholesterol-free, the natural nutriment of high nutrition.
Alcohol soluble protein in walnut protein is made of unique amino acid, wherein branched-chain amino acid and neutral amino acid content
It is higher, it is rare characteristic component in vegetable protein, there is high value of exploiting and utilizing, walnut protein contains 18 kinds of amino
Acid, including eight kinds must amino acid, and arginine, glutamic acid, histidine, tyrosine equal size is relatively high, close to connection
Standard as defined in He Guo food and agricultural organization (FAO) and the World Health Organization (WHO), it is a kind of good vegetable protein.
A kind of five-cereal health-care peptide, chelated iron after various plants albumen multienzyme stepwise discretization, protein peptides can play the battalion of itself
Value is supported, absorption in human body, which has, to be absorbed fastly, and high-efficient, low energy consumption, and polypeptide chelate Organic Iron can be such that iron ion easily absorbs,
Absorb fast, the effect that makes to enrich blood is good.
Preferably, a kind of five-cereal health-care peptide, various plants albumen content meter by weight includes: soybean protein 45
Part, 10 parts of wheat germ protein, 10 parts of zein, 5 parts of peanut protein, 5 parts of walnut protein.
Preferably, five-cereal health-care peptide content meter by weight further includes that vitamin C 1.5- is added in chelated iron step
2.5 parts and vitamin B 1-2 parts.
Preferably, five-cereal health-care peptide content meter by weight further includes 10-25 parts of xylitol.
A kind of five-cereal health-care peptide preparation method, comprising the following steps:
S1: taking soybean protein, wheat germ protein, zein, peanut protein, walnut protein, and solid-liquid ratio g/mL is 1:4-5 addition
Deionized water, stirring and dissolving obtain mixing liquid；
S2: by above-mentioned mixing liquid, solid-liquid ratio g/mL is that neutral proteinase, after being dispersed with stirring, first treatment fluid is added in 1:100；
S3: by above-mentioned just treatment fluid, add NaOH solution to adjust pH value to 8.5, be placed in 55-60 DEG C of water-bath, mixed through high speed shear
Mulser is after 20000-22000r/min handles 5min, and solid-liquid ratio g/mL is plus 1:100, addition alkali protease digest
Time is 3-4h, obtains enzymolysis liquid, keeps the pH value of reaction system constant in reaction process；
S4: by the enzymolysis liquid, boiling water bath 10min enzyme deactivation is cooled to 30 DEG C and adjusts pH value to 4.0 hereinafter, HCl solution is added,
Filtering, obtains supernatant；
S5: by the supernatant, solid-liquid ratio g/ml is that sodium trimetaphosphate is added in 2:100, and NaOH solution is added to adjust pH value to 8.0,
30-40 DEG C of reaction temperature, time 75-90 min obtains reaction solution one；
S6: by the reaction solution one, being added vitamin C, vitamin B, and chalybeate reacts 30-40 min in constant temperature heating water-bath,
And whole nitrogen charging gas shielded, so that the solution in reaction flask is in anaerobic gaseity, obtains reaction solution two；
S7: by the reaction solution two, the dehydrated alcohol with two equal volume of reaction solution is added, filtering precipitates drying, obtains xeraphium
End；
S8: xylitol is added in the dried powder, is uniformly mixed, obtains five-cereal health-care peptide.
Preferably, the neutral proteinase is one or both of protease A S1.398 and protease P rotamex.
Preferably, the basic protein be one of alkali protease Alcalase FG 2.4L and trypsase or
Two kinds.
Preferably, the chalybeate is frerrous chloride.
Preferably, the NaOH solution is 1mol/L.
Preferably, the HCl solution is 1mol/L.
Alkali protease (also known as serine protease) is used as a species specificity restriction endonuclease, mainly acts on certain containing hydrophobic
Property carboxyl peptide bond, the fracture of the peptide bond is so that hydrophobic group exposure；The position of its major catalytic is serine, has reaction
Fireballing feature.
Alkali protease Alcalase FG 2.4L derives from bacillus licheniformis, Glu in cleavable albumen,
The carboxylic end peptide bond of Met, Leu, Tyr, Lys and Gln, and relatively large amino acid side chain, alkali protease act more easily on peptide
Key.
Using multienzyme fractional hydrolysis, content of peptides in hydrolysate can be increased and reduce amino acid content, because identical
Peptide is generated under albumen enzyme effect, restriction enzyme site is similar or identical, between the equal 500Da--1000Da of molecular weight, generates after enzymatic hydrolysis
Small peptide, chelated iron have polypeptide outside the nutritive value of itself, and there are also the effects that benefit iron is enriched blood.
Plant separation protein contains a large amount of phytic acid, therefore sloughs before preparing metallo-chelate the phytic acid in albumen and not only may be used
To improve human body to the absorbability of mineral element, while the ability of protein peptides chelated metal ions can also be improved, eliminate and plant
Albumen and metal ion binding capacity can be improved in the influence of acid and metal ion-chelant, the phosphorylation modification of protein peptides, then
It is allowed in conjunction with frerrous chloride, prepares iron chelating peptide, and the step of finally establishing optimization and reaction condition, reach maximum
The effect of phosphorylation and strongest iron binding capacity.
By carrying out phosphorylation modification to soybean protein isolate using sodium tripolyphosphate, hence it is evident that increase the dissolution speed of albumen
Degree, but solubility and viscosity have no significant change.
The present invention has following technical effect that compared with prior art
A kind of five cereals peptide and preparation method thereof, five-cereal health-care peptide is chelated after multienzyme stepwise discretization iron by various plants albumen
, the various plants albumen includes: soybean protein, wheat germ protein, zein, peanut protein, walnut protein, passes through multienzyme
Hydrolyze method, polypeptide molecular weight between 500-1000Da, polypeptide chelate iron make chalybeate be converted into easily absorption, easily store it is organic
Iron, alcohol precipitation realize the separation of Organic Iron and inorganic iron, make the more preferable chelated iron of polypeptide, and polypeptide can play idiotrophic value, simultaneously
There is the effect of blood-nourishing and iron-replenishing.
Specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment, but embodiment does not do any type of limit to the present invention
It is fixed.Unless stated otherwise, the reagent that the present invention uses, method and apparatus are the art conventional reagent, method and apparatus.
Unless stated otherwise, agents useful for same and material of the present invention are commercially available.
Embodiment 1
A kind of five-cereal health-care peptide, by weight content meter include: 40 parts of soybean protein, 10 parts of wheat germ protein, zein 10-20
Part, 5 parts of peanut protein, 5 parts of walnut protein, 1.5 parts of vitamin C, 1 part of vitamin B, 7 parts of frerrous chloride, xylitol 10
Part.
A kind of five-cereal health-care peptide preparation method, comprising the following steps:
S1: taking soybean protein, wheat germ protein, zein, peanut protein, walnut protein, and solid-liquid ratio g/mL is that 1:4 addition is gone
Ionized water, stirring and dissolving obtain mixing liquid；
S2: by above-mentioned mixing liquid, solid-liquid ratio g/mL is that protease A S1.398, after being dispersed with stirring, first treatment fluid is added in 1:100；
S3: by above-mentioned just treatment fluid, add 1mol/L NaOH solution to adjust pH value to 8.5,55 DEG C of water-bath are placed in, through high speed shear
For Mixingemulsificationmachine after 20000r/min handles 5min, solid-liquid ratio g/mL is to add 1:100, and alkali protease is added
Alcalase FG 2.4L, enzymolysis time 3-4h obtain enzymolysis liquid, add and keep reaction in 1mol/L NaOH solution reaction process
The pH value of system is constant；
S4: by above-mentioned enzymolysis liquid, boiling water bath 10min enzyme deactivation is cooled to 30 DEG C and adjusts pH hereinafter, 1mol/L HCl solution is added
For value to 4.0, filtering obtains supernatant；
S5: by above-mentioned supernatant, solid-liquid ratio g/ml is that sodium trimetaphosphate is added in 2:100, and 1mol/L NaOH solution is added to adjust pH value
8.0,30 DEG C of reaction temperature, 75 min of time obtains reaction solution one；
S6: by above-mentioned reaction solution one, being added vitamin C, vitamin B, and frerrous chloride reacts 30 min in constant temperature heating water-bath,
And whole nitrogen charging gas shielded, so that the solution in reaction flask is in anaerobic gaseity, obtains reaction solution two；
S7: by above-mentioned reaction solution two, the dehydrated alcohol with two equal volume of reaction solution is added, filtering precipitates drying, obtains xeraphium
End；
S8: xylitol is added in above-mentioned dried powder, is uniformly mixed, obtains five-cereal health-care peptide.
Embodiment 2
A kind of five-cereal health-care peptide, by weight content meter include: 50 parts of soybean protein, 20 parts of wheat germ protein, 20 parts of zein,
10 parts of peanut protein, 10 parts of walnut protein, 2.5 parts of vitamin C, 1.5 parts of vitamin B, 11 parts of chalybeate, 25 parts of xylitol.
A kind of five-cereal health-care peptide preparation method, comprising the following steps:
S1: taking soybean protein, wheat germ protein, zein, peanut protein, walnut protein, and solid-liquid ratio g/mL is 1:4.5 addition
Deionized water, stirring and dissolving obtain mixing liquid；
S2: by above-mentioned mixing liquid, solid-liquid ratio g/mL is that protease P rotamex is added in 1:100, after being dispersed with stirring, is just handled
Liquid；
S3: by above-mentioned just treatment fluid, add 1mol/L NaOH solution to adjust pH value to 8.5,60 DEG C of water-bath are placed in, through high speed shear
For Mixingemulsificationmachine after 21000r/min handles 5min, solid-liquid ratio g/mL is to add 1:100, and trypsase, enzymolysis time is added
For 3-4h, enzymolysis liquid is obtained, adds in 1mol/L NaOH solution reaction process and keeps the pH value of reaction system constant；
S4: by above-mentioned enzymolysis liquid, boiling water bath 10min enzyme deactivation is cooled to 30 DEG C and adjusts pH hereinafter, 1mol/L HCl solution is added
For value to 4.0, filtering obtains supernatant；
S5: by above-mentioned supernatant, solid-liquid ratio g/ml is that sodium trimetaphosphate is added in 2:100, and 1mol/LNaOH is added and adjusts pH value
8.0,35 DEG C of reaction temperature, time 80min obtains reaction solution one；
S6: by above-mentioned reaction solution one, being added vitamin C, vitamin B, and frerrous chloride reacts 35 min in constant temperature heating water-bath,
And whole nitrogen charging gas shielded, so that the solution in reaction flask is in anaerobic gaseity, obtains reaction solution two；
S7: by above-mentioned reaction solution two, the dehydrated alcohol with two equal volume of reaction solution is added, filtering precipitates drying, obtains xeraphium
End；
S8: xylitol is added in above-mentioned dried powder, is uniformly mixed, obtains five-cereal health-care peptide.
Embodiment 3
A kind of five-cereal health-care peptide, by weight content meter include: 45 parts of soybean protein, 10 parts of wheat germ protein, 10 parts of zein,
5 parts of peanut protein, 5 parts of walnut protein, 2 parts of vitamin C, 2 parts of vitamin B, 8 parts of frerrous chloride, 12 parts of xylitol.
A kind of five-cereal health-care peptide preparation method, comprising the following steps:
S1: taking soybean protein, wheat germ protein, zein, peanut protein, walnut protein, and solid-liquid ratio g/mL is 1:5 addition
Deionized water, stirring and dissolving obtain mixing liquid；
S2: by above-mentioned mixing liquid, solid-liquid ratio g/mL is that neutral proteinase, after being dispersed with stirring, first treatment fluid is added in 1:100；
S3: by above-mentioned just treatment fluid, add 1mol/L NaOH solution to adjust pH value to 8.5,60 DEG C of water-bath are placed in, through high speed shear
For Mixingemulsificationmachine after 22000r/min handles 5min, solid-liquid ratio g/mL is plus 1:100 alkali protease to be added, when enzymatic hydrolysis
Between be 4h, obtain enzymolysis liquid, add in 1mol/L NaOH solution reaction process keep reaction system pH value it is constant；
S4: by above-mentioned enzymolysis liquid, boiling water bath 10min enzyme deactivation is cooled to 30 DEG C and adjusts pH hereinafter, 1mol/L HCl solution is added
For value to 4.0, filtering obtains supernatant；
S5: by above-mentioned supernatant, solid-liquid ratio g/ml is that sodium trimetaphosphate is added in 2:100, and 1mol/L NaOH solution is added to adjust pH value
8.0,40 DEG C of reaction temperature, 90 min of time obtains reaction solution one；
S6: by above-mentioned reaction solution one, being added vitamin C, vitamin B, and frerrous chloride reacts 40 min in constant temperature heating water-bath,
And whole nitrogen charging gas shielded, so that the solution in reaction flask is in anaerobic gaseity, obtains reaction solution two；
S7: by above-mentioned reaction solution two, the dehydrated alcohol with two equal volume of reaction solution is added, filtering precipitates drying, obtains xeraphium
End；
S8: xylitol is added in above-mentioned dried powder, is uniformly mixed, obtains five-cereal health-care peptide.
Comparative example 1
A kind of five-cereal health-care peptide, by weight content meter include: 50 parts of soybean protein, 20 parts of wheat germ protein, 20 parts of zein,
10 parts of peanut protein, 10 parts of walnut protein, 2.5 parts of vitamin C, 1.5 parts of vitamin B, 11 parts of frerrous chloride, xylitol 25
Part.
A kind of five-cereal health-care peptide preparation method, comprising the following steps:
S1: taking soybean protein, wheat germ protein, zein, peanut protein, walnut protein, and solid-liquid ratio g/mL is that 1:4 addition is gone
Ionized water, stirring and dissolving obtain mixing liquid；
S2: by above-mentioned mixing liquid, add 1mol/L NaOH solution to adjust pH value to 8.5,55 DEG C of water-bath are placed in, through high speed shear
Mixingemulsificationmachine is after 22000r/min handles 5min, and solid-liquid ratio g/mL is plus Papain is added in 1:100, and enzymolysis time is
3-4h obtains enzymolysis liquid, adds in 1mol/L NaOH solution reaction process and keeps the pH value of reaction system constant；
S3: by above-mentioned enzymolysis liquid, boiling water bath 10min enzyme deactivation is cooled to 30 DEG C and adjusts pH hereinafter, 1mol/L HCl solution is added
For value to 4.0, filtering obtains supernatant；
S4: by above-mentioned supernatant, solid-liquid ratio g/ml is that sodium trimetaphosphate is added in 2:100, and 1mol/L NaOH solution adjusts pH value
8.0,40 DEG C of reaction temperature, 90 min of time obtains reaction solution one.
S5: by above-mentioned reaction solution one, being added vitamin C, vitamin B, alkali protease Alcalase FG 2.4L,
30 min, and whole nitrogen charging gas shielded are reacted in constant temperature heating water-bath, so that the solution in reaction flask is in anaerobic gaseity, are obtained
Reaction solution two；
S6: by above-mentioned reaction solution two, the dehydrated alcohol with two equal volume of reaction solution is added, filtering precipitates drying, obtains xeraphium
End；
S7: xylitol is added in above-mentioned dried powder, is uniformly mixed, obtains five-cereal health-care peptide.
Comparative example 2
A kind of five-cereal health-care peptide preparation method, comprising the following steps:
S1: taking soybean protein, wheat germ protein, zein, peanut protein, walnut protein, and solid-liquid ratio g/mL is that 1:4 addition is gone
Ionized water, stirring and dissolving obtain mixing liquid；
S2: by above-mentioned just treatment fluid, add 1mol/L NaOH solution to adjust pH value to 8.5,55 DEG C of water-bath are placed in, through high speed shear
Mixingemulsificationmachine is after 22000r/min handles 5min, and solid-liquid ratio g/mL is plus alkali protease Alcalase is added in 1:100
FG 2.4L, enzymolysis time 3-4h, obtains enzymolysis liquid, adds the pH that reaction system is kept in 1mol/L NaOH solution reaction process
It is worth constant；
S3: by above-mentioned enzymolysis liquid, boiling water bath 10min enzyme deactivation is cooled to 30 DEG C and adjusts pH hereinafter, 1mol/L HCl solution is added
For value to 4.0, filtering obtains supernatant；
S4: by above-mentioned supernatant, being added vitamin C, vitamin B, frerrous chloride, add 1mol/L NaOH solution adjust pH value to
7.0,30 min, and whole nitrogen charging gas shielded are reacted in constant temperature heating water-bath, so that the solution in reaction flask is in anaerobic vaporous
State obtains reaction solution；
S5: by above-mentioned reaction solution, the dehydrated alcohol with reaction solution equal volume is added, filtering precipitates drying, obtains dried powder；
S6: xylitol is added in above-mentioned dried powder, is uniformly mixed, obtains five-cereal health-care peptide.
Application experiment example 1
SDS-PAGE electrophoretic determination protein relative molecular mass
(1) instrument, raw materials and reagents
Instrument: vertical version type electrophoresis tank；D.C. regulated power supply；50uL pipette tips, glass plate, water-bath, staining trough, beaker, pipette
Pipe, dropper.
Raw material: 0.5mg/mL sample solution
Reagent: Tris-HCl pH of buffer 8.9, Tris-HCl pH of buffer 6.7,30% separation gel liquid storage, 10% concentration glue liquid storage,
10%SDS solution, dyeing liquor, destainer.
(2) experimental procedure
1. installing electrophoresis tank.
2. matching glue.
3. preparing gel slab.
4. sample treatment and sample-adding
0.5mg/mL sample solution and standard protein liquid boiling water bath are heated 3 minutes, be cooled to room temperature it is spare, by 15uL sample
It carefully is added to gel spill sample trench bottom, to all add sample in all spill sample cells, starts electrophoresis.
5. electrophoresis
Electrophoresis apparatus is opened, electric current is transferred to 10mA, when sample separation, electric current is adjusted to 25mA, when blue dyes migrates the bottom of to
Portion, electric current are set to zero, and power supply is closed, and pull out fixed plate, are taken out glass plate, are gently pried open one block of glass with blade and remove,
Offset plate one end makes marks, dyeing.
6. dyeing and decoloration
Dyeing liquor is poured into culture dish, dyeing 1h or so for several times, then with destainer is decolourized with distilled water rinsing, until albumen
Zone is clear, measures each band at a distance from gel top with ruler.
7. calculating
It is mapped with the logarithm of standard protein molecular weight to relative mobility, obtains standard curve, moved according to sample to be tested is opposite
Shifting rate can find out its molecular weight from standard curve.
Relative mobility Rm=sample migration distance (cm)/dye migration distance (cm)
The experimental results showed that gained embodiment 1-3 protein relative molecular weight distribution is between 500-1000Da.
Experimental example 1
80 anemia mices are selected, every 10/group is quiet through tail with five-cereal health-care peptide 40mg/d stomach-filling 30 days after adapting to 3 d
Arteries and veins takes blood, detects blood routine, calculates average value.Million/mm of the normal Erythrocytes 9.3 (7.7 ~ 12.5) of mouse3, blood red
Protein 14 .8 (10 ~ 19) g/100ml.
Erythrocyte number is most in blood, in the Wilson Begonia Rhizome of needle point size, can there is millions of, and shape is oblate, center
Slightly in recess.Contain a kind of special proteins matter that can carry oxygen and carbon dioxide of red, referred to as hemoglobin into the cell, it is red thin
The function of born of the same parents is exactly to convey gas.Erythrocyte number increase and decrease and hemoglobin increase and decrease are almost the same, and the two counts, and metering is lower than just
It often, is exactly the anaemia being usually commonly called as.Anaemia is related to many factors, does red blood cell count(RBC) and hemoglobinometry
1 anemia mice red blood cell of table and hemoglobin variation
As shown in Table 1, embodiment 1-3 effect of enriching blood is good.
Such as " comprising ", "comprising", the word of " having " etc. are open vocabulary, are referred to " including but not limited to ", and can
It is used interchangeably with it.Vocabulary "or" and "and" used herein above refer to vocabulary "and/or", and can be used interchangeably with it, unless on
It is such for being hereafter explicitly indicated not.Vocabulary " such as " used herein above refers to phrase " such as, but not limited to ", and can exchange with it
It uses.
In addition, as used herein, the "or" instruction separation used in the enumerating of the item started with "at least one"
It enumerates, such as enumerating for " at least one of A, B or C " means A or B or C or AB or AC or BC or ABC(i.e. A and B and C).
In addition, wording " exemplary " does not mean that the example of description is preferred or more preferable than other examples.
The above description of disclosed aspect is provided so that any person skilled in the art can make or use this
It is open.Various modifications in terms of these are readily apparent to those skilled in the art, and are defined herein
General Principle can be applied to other aspect without departing from the scope of the present disclosure.Therefore, the disclosure is not intended to be limited to
Aspect shown in this, but according to principle disclosed herein and the consistent widest range of novel feature.
Above description is had been presented for for purposes of illustration and description.In addition, this description is not intended to the reality of the disclosure
It applies example and is restricted to form disclosed herein.Although already discussed above multiple exemplary aspects and embodiment, this field skill
Its certain modification will be recognized in art personnel, modifies, and changes, addition and sub-portfolio.
Claims (10)
1. a kind of five-cereal health-care peptide, which is characterized in that the five-cereal health-care peptide is by various plants albumen after multienzyme stepwise discretization
Chelated iron is made, and various plants albumen content meter by weight includes: 40-50 parts of soybean protein, wheat germ protein 10-20
Part, 10-20 parts of zein, 5-10 parts of peanut protein, 5-10 parts of walnut protein.
2. five-cereal health-care peptide according to claim 1, which is characterized in that various plants albumen content meter packet by weight
It includes: 45 parts of soybean protein, 10 parts of wheat germ protein, 10 parts of zein, 5 parts of peanut protein, 5 parts of walnut protein.
3. five-cereal health-care peptide according to claim 1, which is characterized in that the five-cereal health-care peptide content meter by weight also wraps
It includes and is added vitamin C 1.5-2.5 parts and vitamin B 1-2 parts in chelated iron step.
4. five-cereal health-care peptide according to claim 1, which is characterized in that the five-cereal health-care peptide content meter by weight also wraps
Include 10-25 parts of xylitol.
5. a kind of preparation method of five-cereal health-care peptide described in claim 1-4, which comprises the following steps:
S1: taking soybean protein, wheat germ protein, zein, peanut protein and walnut protein, is added with solid-liquid ratio g/mL for 1:4-5
Enter deionized water, stirring and dissolving obtains mixing liquid；
S2: by above-mentioned mixing liquid, solid-liquid ratio g/mL is that neutral proteinase is added in 1:100, after being dispersed with stirring, obtains just treatment fluid；
S3: by above-mentioned just treatment fluid, add NaOH solution to adjust pH value to 8.5, be placed in 55-60 DEG C of water-bath, mixed through high speed shear
For mulser after 20000-22000r/min handles 5min, solid-liquid ratio g/mL is 1:100, alkali protease is added, when enzymatic hydrolysis
Between be 3-4h, obtain enzymolysis liquid, keep the pH value of reaction system constant in reaction process；
S4: by the enzymolysis liquid, boiling water bath 10min enzyme deactivation is cooled to 30 DEG C and adjusts pH value to 4.0 hereinafter, HCl solution is added,
Filtering, obtains supernatant；
S5: by the supernatant, solid-liquid ratio g/ml is that sodium trimetaphosphate is added in 2:100, and NaOH solution is added to adjust pH value to 8.0,
30-40 DEG C of reaction temperature, time 75-90 min obtains reaction solution one；
S6: by the reaction solution one, being added vitamin C, vitamin B and chalybeate, 30-40 min reacted in constant temperature heating water-bath,
And whole nitrogen charging gas shielded, so that the solution in reaction flask is in anaerobic gaseity, obtains reaction solution two；
S7: by the reaction solution two, the dehydrated alcohol with two equal volume of reaction solution is added, filtering precipitates drying, obtains xeraphium
End；
S8: xylitol is added in the dried powder, is uniformly mixed, obtains five-cereal health-care peptide.
6. method according to claim 5, which is characterized in that the neutral proteinase is protease A S1.398 and protease
One or both of Protamex.
7. method according to claim 5, which is characterized in that the basic protein is alkali protease Alcalase FG
One or both of 2.4L and trypsase.
8. method according to claim 5, which is characterized in that the chalybeate is frerrous chloride.
9. method according to claim 5, which is characterized in that the NaOH solution is 1mol/L.
10. method according to claim 5, which is characterized in that the HCl solution is 1mol/L.
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