CN110117526A - A kind of novel fermentation device and its application in ethyl alcohol is prepared in Immobilized yeast - Google Patents
A kind of novel fermentation device and its application in ethyl alcohol is prepared in Immobilized yeast Download PDFInfo
- Publication number
- CN110117526A CN110117526A CN201910372735.XA CN201910372735A CN110117526A CN 110117526 A CN110117526 A CN 110117526A CN 201910372735 A CN201910372735 A CN 201910372735A CN 110117526 A CN110117526 A CN 110117526A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- tank
- fermentation tank
- circulating line
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 105
- 230000004151 fermentation Effects 0.000 title claims abstract description 105
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 14
- 235000019441 ethanol Nutrition 0.000 title claims abstract description 11
- 239000007921 spray Substances 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims abstract description 17
- 239000000463 material Substances 0.000 claims abstract description 16
- 238000009434 installation Methods 0.000 claims abstract description 13
- 238000007599 discharging Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 37
- 235000007164 Oryza sativa Nutrition 0.000 claims description 16
- 235000009566 rice Nutrition 0.000 claims description 16
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 11
- 240000003183 Manihot esculenta Species 0.000 claims description 10
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 10
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 8
- 235000005822 corn Nutrition 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 8
- -1 polyethylene Polymers 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000000835 fiber Substances 0.000 claims description 4
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 235000013379 molasses Nutrition 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 230000002035 prolonged effect Effects 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 241000588902 Zymomonas mobilis Species 0.000 claims description 2
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 2
- 239000004800 polyvinyl chloride Substances 0.000 claims description 2
- 238000005215 recombination Methods 0.000 claims description 2
- 230000006798 recombination Effects 0.000 claims description 2
- 239000012209 synthetic fiber Substances 0.000 claims description 2
- 229920002994 synthetic fiber Polymers 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000012138 yeast extract Substances 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 2
- 230000032683 aging Effects 0.000 claims 1
- 239000011344 liquid material Substances 0.000 claims 1
- 241000209094 Oryza Species 0.000 description 14
- 238000012546 transfer Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000001179 sorption measurement Methods 0.000 description 4
- 102000004139 alpha-Amylases Human genes 0.000 description 3
- 108090000637 alpha-Amylases Proteins 0.000 description 3
- 229940024171 alpha-amylase Drugs 0.000 description 3
- 108010089934 carbohydrase Proteins 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000002657 fibrous material Substances 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011169 microbiological contamination Methods 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 238000011090 industrial biotechnology method and process Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of novel fermentation devices, including fermentation tank, vertical stirring device, fixation support and the circulating line for feeding and discharging for the tank that ferments；Several feeding spray nozzles are equipped on the side wall of fermentation tank, for tank charging of fermenting；Fermentor tank base is equipped with discharge port, for tank discharging of fermenting；Circulating pump is equipped between discharge port and feeding spray nozzle, and it connects to form circulation loop by circulating line, so that the material in fermentation tank enters circulating line by discharge port, under the driving of circulating pump, circulating fermentation in fermentation tank is again introduced by feeding spray nozzle；It is additionally provided with feed pipe and drainage conduit on circulating line, is respectively equipped with check valve in feed pipe and drainage conduit；Fermentation medium is imported into circulating line by feed pipe, and is entered in fermentation tank through feeding spray nozzle；Tunning is discharged through circulating line by drainage conduit.The present invention also provides the installation for fermentings to prepare the application in ethyl alcohol in Immobilized yeast.
Description
Technical field
The invention belongs to industrial biotechnology fields, and in particular to a kind of novel fermentation device and its send out in fixed yeast
Ferment prepares the application in ethyl alcohol.
Background technique
With natural resources scarcity and environmental problem it is outstanding day by day, the status of biofermentation technique is also increasingly heavier
It wants, also achieves fast development in recent years.Immobilization technology becomes important research with its numerous advantage in biofermentation
Hot spot.Although immobilization technology research has longer history, really applied to the very few of industrialized production.Study carefully its original
Cause, mainly entrapment media mechanical strength is low, and mass transfer effect is poor, is not easy to operation etc..We have applied for that surface is fixed before
Change yeast fermentation technique (such as patent No. ZL201210538977.X), i.e., the fibrous material of specially treated is attached to support rib
Frame, and be filled into bioreactor, then yeast cells is fixed on the fibrous material in bioreactor, to realize
The purpose of Immobilized yeast.The technology used carrier prolonged and repeated can utilize, and fermentation efficiency significantly improves, and stability is very
By force, but this method haves the defects that certain, and industrialization amplification installation is complicated, and input cost is higher, and maintenance is relatively difficult.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of novel fermentation devices, prepare second by Immobilized yeast
Alcohol, to solve the problems such as of the existing technology ineffective.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
A kind of novel fermentation device, including fermentation tank, vertical stirring device, fixation support and be used for fermentor
The circulating line of tank body charging and discharging；
Wherein, the vertical stirring device is located on the central axis of fermentor tank interior, is used for stirred fermentor tank body
Interior material；
The fixation support is filled in fermentation tank；
Several feeding spray nozzles are equipped on the side wall of the fermentation tank, for tank charging of fermenting；Fermentor tank
Body bottom is equipped with discharge port, for tank discharging of fermenting；It is equipped with circulating pump between the discharge port and feeding spray nozzle, and passes through
Circulating line connects to form circulation loop, so that the material in fermentation tank enters circulating line by discharge port, is recycling
Under the driving of pump, circulating fermentation in fermentation tank is again introduced by feeding spray nozzle；
It is additionally provided with feed pipe and drainage conduit on the circulating line, is respectively equipped with check valve in feed pipe and drainage conduit；It is logical
It crosses feed pipe and fermentation medium is imported into circulating line, and enter in fermentation tank through feeding spray nozzle；Tunning is through recycling
Pipeline is discharged by drainage conduit.
Further, the direction of feeding spray nozzle ejection liquid is horizontal by 40-60 ° of angle, with fermentation tank
The tangential direction of inner wall is in 30-60 ° of angle, to reach optimal mass transfer effect.
Wherein, the fixation support is Ball Grid carrier, and material is polyvinyl chloride or polyethylene, density 0.9
~1g/cm3, diameter is 15~25cm, is internally provided with several parallel round sheet adsorbing mediums, the adsorbing medium diameter
For 12~25cm, material is plant fiber or synthetic fibers.
The flase floor of several different heights is transversely provided in the fermentation tank, adjacent two pieces of flase floors are one group, it
Between Ball Grid carrier prolonged together by lontitudinal series by cord, be 2~6 with spacing between a string of Ball Grid carrier
Cm, it is adjacent two string between spacing be 3~8cm.
The vertical stirring device includes motor, flat paddle leaf and pusher blade, and the motor is located at fermentation tank top
Portion, for driving the rotation of flat paddle leaf and pusher blade；The flat paddle leaf is located at the nearly top of fermentation tank；The propulsion
Formula blade is at the neutral gear between two adjacent groups flase floor.
Preferably, the fermentation tank ratio of height to diameter is 1.2~2:1, and pot bottom is circular cone type or oblique low-head.
The present invention also provides above-mentioned installation for fermentings to prepare the application in ethyl alcohol in Immobilized yeast, specifically includes as follows
Step:
S1: fermentation strain is adsorbed in fixation support, is then filled into fermentation tank；
S2: it is pumped into fermentation liquid into fermentation tank, is stirred, circulating fermentation；
S3: after fermentation, fermentation liquid is discharged, again the fermentation medium of stream plus hair.
Specifically, the fermentation strain is saccharomyces cerevisiae, active Angel Yeast powder or recombination zymomonas mobilis；It is described
Fermentation liquid is human configuration culture medium, corn powder saccharification liquid, cassava saccharified liquid, saccharified liquid, stalk hydrolyzate or the sugar for being aged rice
Any one or the combination of several of them in honey；Wherein, human configuration culture medium prescription are as follows: glucose 180-250g/L, peptone
2-5g/L, yeast extract 2-5g/L, pH 4.0-5.0；The corn powder saccharification liquid, cassava saccharified liquid, the saccharified liquid for being aged rice
Material water quality ratio is 2.2~3:1；In the stalk hydrolyzate, feed liquid mass ratio is 1:2~6, pH 4.2- after delignification
4.5；The initial total sugar of the molasses is 180-240g/L.
Preferably, the revolving speed 10-30r/min of the stirring.
The flow of the circulation is 0.5-2BV/h.
The utility model has the advantages that
1, fixation support production used in the immobilization technology that the present invention uses is simple, low in cost, easy for installation, unit
Space filling sheet-like fiber medium amount is big, and adsorption effect is preferable.
2, the present invention uses suspension type fixation support, so that carrier is uniformly distributed in tank, while sheet medium is in ball
It is interior to be placed in parallel with axial, carrier is effectively reduced to the interception function with slag material, so that discharge is more thorough, to a certain degree
On reduce microbiological contamination probability.
3, the stirring that the present invention uses adds endless form, effectively prevents the carrier ball hung in tank to the shadow of mass-and heat-transfer
It rings.Middle layer and the design of lower layer's stirrer paddle push type are conducive to slag logistics the central region in tank and form upward Scroll-tupe to push away
Power, the design of upper layer annular feed pipe are conducive to recycle feed and form downward Scroll-tupe in tank level upper end periphery to push away
Power.So that the state of flowing is presented in whole material in tank.
4, the present invention uses based on stirring fermentation, and the immobilization fermentation than generally relying on circulation mass transfer more saves power consumption.
Detailed description of the invention
The present invention is done with reference to the accompanying drawings and detailed description and is further illustrated, of the invention is above-mentioned
And/or otherwise advantage will become apparent.
Fig. 1 is the overall structure diagram of the installation for fermenting；
Fig. 2 a and 2b are CFD (computational fluid dynamics) mass transfer effect simulation drawing that circulation is not added in embodiment 1；
Fig. 3 a, 3b and 3c are that 2 recycle feed spray head of embodiment is in the CFD mass transfer effect simulation drawing under close call.
Specific embodiment
According to following embodiments, the present invention may be better understood.
Structure depicted in Figure of description, ratio, size etc., only to cooperate the revealed content of specification, with
Understand for those skilled in the art and read, be not intended to limit the invention enforceable qualifications, therefore does not have technically
Essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size can be generated not influencing the present invention
Under effect and the purpose that can reach, should all still it fall in the range of disclosed technology contents can cover.Meanwhile
Cited such as "upper", "lower", "front", "rear", " centre " term in this specification are merely convenient to being illustrated for narration, and
It is non-that to limit the scope of the invention, relativeness is altered or modified, under the content of no substantial changes in technology, when
Also it is considered as the enforceable scope of the present invention.
As shown in Figure 1, the installation for fermenting includes fermentation tank 1, vertical stirring device 2, fixation support 3 and is used for
The tank that ferments is fed and the circulating line of discharging.
Wherein, fermentation 1 pot bottom of tank is oblique low-head, and bottom is equipped with sediment ejection opening 8.
The vertical stirring device 2 is located on the central axis inside fermentation tank 1, in stirred fermentor tank body 1
Material.
The fixation support 3 is filled in fermentation tank 1.
The side wall upper, middle and lower of the fermentation tank 1 are equipped with four feeding spray nozzles 4 respectively, for the tank 1 that ferments
Charging, each ring feeding spray nozzle 4 is connected on the pipeline outside fermentation tank, and valve 41 is equipped with outside feeding spray nozzle 4；
1 bottom of tank ferment equipped with discharge port 11, for fermenting, tank 1 discharges；Between the discharge port 11 and feeding spray nozzle 4
It connects to form circulation loop equipped with circulating pump 5, and by circulating line, so that the material in fermentation tank 1 passes through discharge port
11 enter circulating line, under the driving of circulating pump 5, are again introduced into circulating fermentation in fermentation tank 1 by feeding spray nozzle 4.
It is additionally provided with feed pipe 6 and drainage conduit 7 on the circulating line, is respectively equipped on feed pipe 6 and drainage conduit 7 unidirectional
Valve；Fermentation medium is imported into circulating line by feed pipe 6, and is entered in fermentation tank 1 through feeding spray nozzle 4；Fermentation produces
Object is discharged through circulating line by drainage conduit 7.
The fixation support 3 is Ball Grid carrier, and material is polyethylene, density 0.94g/cm3.It will be cut into straight
Each 2 pieces (thickness 0.15cm) of the round sheet cotton fiber cloth of diameter 16cm and 19cm, is placed in parallel and is fixed on Ball Grid carrier
Interior formation adsorbing medium, spacing about 4cm, Ball Grid carrier runs through a round tube, for tandem vector of restricting, places in each ball
Fibre weight about 49g.
The vertical stirring device 2 includes that motor 21, flat paddle leaf 22 and pusher blade 23, the motor 21 are located at fermentation
1 top of tank, for driving the rotation of flat paddle leaf 22 and pusher blade 23；The flat paddle leaf 22 is located at fermentation tank 1
Nearly top；The push type blade 23 is at the neutral gear between two adjacent groups flase floor.
Embodiment 1
The fermentation jar structure and mass transfer analogy of circulation is not added:
It is divided into two layers in 2000 tons of fermentors of ratio of height to diameter 1.2, concatenated immobilization ball is fixed on every layer of upper lower grid plate
Both ends, between the upper and lower away from 4cm, horizontal direction spacing 5cm, practical loadings about 10500.Bottom, middle layer push type blade 23 are straight
Diameter 5.33m, with head width 43cm 30 ° of horizontal direction angle big, small head width 10cm, 3 blades.Top horizontal blade, rotation are straight
Diameter 5m, disk diameter 3.5m, blade long 1.5m, wide 0.1m, 3 piece blades vertical with horizontal direction.In revolving speed 12r/min condition
Under, mass transfer situation is as shown in Figure 2 a and 2 b, upper layer (A) mean flow rate 0.16m/s, and lower layer (B) mean flow rate 0.14m/s is whole
Body mean flow rate 0.15m/s, mass transfer effect is preferable, effectively prevents material deposition.
The fermentation jar structure and mass transfer analogy of embodiment 2 plus circulation
Recycle feed is put in from tank skin, is in 45 degree of design nozzle (as shown in Figure 3a) with downward 30 degree and with tangent line,
His mode is the same as embodiment 1.Under the conditions of revolving speed 12r/min, mass transfer situation is as shown in figures 3 b and 3 c.Upper layer (A) mean flow rate
0.17m/s, lower layer (B) mean flow rate 0.16m/s, ensemble average flow velocity 0.165m/s, mass transfer effect are more excellent.
Embodiment 3 is using cassava liquefied fermented glutinous rice culture medium as fermentation raw material
By fixation support be loaded on reactor in embodiment 2, be 900m with internal circulating load3/ h fixes seed liquor 48h
(seed culture medium is cassava converted mash, as hereinafter described).Until 2.5 hundred million/ml of thallus adsorption concentration.Then cassava liquid is prepared
Change wine with dregs: solid-liquid ratio 1:2.3, add 90 DEG C of α-amylase (16U/g cassava dry weight) to liquefy 1.5 hours, immobilization seed is discharged after cooling
Waste liquid.It flows again plus cassava liquefied fermented glutinous rice (initial total sugar 226.0g/L, and carbohydrase (180U/g cassava dry weight), liquid amount is added
85%, circulating fermentation is carried out, wherein temperature is 33 DEG C, fermentation liquid pH4.3, flow velocity 900m3/ h, speed of agitator 12r/min, fermentation
Terminate, exhaust fermentation liquid, feed liquid total residual is less than 8%, than fermentation residual in existing method (application number 201810321244.8)
Amount has dropped 15% or more, hence it is evident that reduces microbiological contamination risk, improves utilization rate of equipment and installations.Again it is pumped into new fermentation liquid.It can be steady
Surely 40 batches or more are run, the average fermentation period is 36h, and reaction density reaches 109.2g/L, fermentation (inoculum concentration more free than tradition
25%) it shortens 8 hours or more.Alcohol yied reaches 3.03gL-1h-1, 94.5% or more average conversion.
Embodiment 4 is to be aged rice as fermentation raw material
Equally by fixation support (carrier ball polypropylene) be loaded on reactor in embodiment 2, be with internal circulating load
900m3/ h fixes seed liquor 48h (seed culture medium is rice converted mash, as hereinafter described).Until thallus adsorption concentration 300,000,000
A/ml.Rice liquefied fermented glutinous rice: solid-liquid ratio 1:1.8 is prepared, adds 86 DEG C of α-amylase (15U/g rice dry weight) to liquefy 2 hours, after cooling
Immobilization seed waste liquid is discharged.It flows again and adds rice liquefied fermented glutinous rice, and carbohydrase (180U/g rice dry weight) is added, liquid amount 85%,
Circulating fermentation is carried out, wherein temperature is 32 DEG C, fermentation liquid pH4.3, and initial cycle flow velocity is 800m3/ h, mid-term can reduce flow
To 400m3/h.The equal fermentation period of 8 criticize flats is 32h (216.7 g/L of initial total sugar), and reaction density reaches 104.1g/L, specific ionization
Fermentation shortens 6 hours.Alcohol yied reaches 3.25gL-1h-1, average conversion 94%.Fermentation energy loss-rate existing method
(ZL201210538977.X) the fermentation energy consumption of carrier type of feed reduces 50% or so.
Embodiment 5 is using corn as fermentation raw material
Equally by fixation support (carrier ball polypropylene) be loaded on reactor in embodiment 2, in a manner of stirring
Fixed seed liquor 44 hours (seed culture medium is corn mash, as hereinafter described).Until thallus adsorption concentration 2.8 hundred million/
ml.Corn liquefied fermented glutinous rice: solid-liquid ratio 1:2.45 is prepared, adds 86 DEG C of α-amylase (15U/g dry weight of maize) to liquefy 2 hours, cooling heel row
Immobilization seed waste liquid out.It flows again and adds corn liquefied fermented glutinous rice, and carbohydrase (180U/g dry weight of maize) is added, liquid amount 85%, into
Row circulating fermentation, wherein temperature is 34 DEG C, fermentation liquid pH4.3, stirring fermentation (revolving speed 16r/min).Fermentation can stable operation 10
Batch, the average fermentation period is 28 hours (initial total sugar 209.6g/L), and reaction density reaches 101.78g/L, specific ionization fermentation contracting
It is 6 hours short.Alcohol yied reaches 3.64gL-1h-1, average conversion 95%.Than stirring plus endless form (internal circulating load 800m3/
H) residue residual rate increases 20% or so, but energy consumption reduces 70% or more.
The think of of the application in ethyl alcohol is prepared the present invention provides a kind of novel fermentation device and its in Immobilized yeast
Road and method, there are many method and the approach for implementing the technical solution, the above is only a preferred embodiment of the present invention,
It should be pointed out that for those skilled in the art, without departing from the principle of the present invention, can also do
Several improvements and modifications out, these modifications and embellishments should also be considered as the scope of protection of the present invention.What is be not known in the present embodiment is each
The available prior art of component part is realized.
Claims (10)
1. a kind of novel fermentation device, which is characterized in that carried including fermentation tank (1), vertical stirring device (2), immobilization
Body (3) and the circulating line for feeding and discharging for the tank that ferments；
Wherein, the vertical stirring device (2) is located on the internal central axis of fermentation tank (1), is used for stirred fermentor tank
Material in body (1)；
The fixation support (3) is filled in fermentation tank (1)；
Several feeding spray nozzles (4) are equipped on the side wall of fermentation tank (1), are fed for the tank that ferments (1)；Fermentation
Tank (1) bottom is equipped with discharge port (11), discharges for the tank that ferments (1)；The discharge port (11) and feeding spray nozzle (4)
Between be equipped with circulating pump (5), and connect to form circulation loop by circulating line, so that the material in fermentation tank (1) leads to
It crosses discharge port (11) and fermentor tank is again introduced by feeding spray nozzle (4) under the driving of circulating pump (5) into circulating line
Body (1) interior circulating fermentation；
There are also feed pipe (6) and drainage conduit (7) on the circulating line, it is respectively equipped on feed pipe (6) and drainage conduit (7) unidirectionally
Valve；Fermentation medium is imported into circulating line by feed pipe (6), and is entered in fermentation tank (1) through feeding spray nozzle (4)；
Tunning is discharged through circulating line by drainage conduit (7).
2. installation for fermenting according to claim 1, which is characterized in that the feeding spray nozzle (4) sprays the direction of liquid
Horizontal by 40-60 ° of angle, the tangential direction with fermentation tank (1) inner wall is in 30-60 ° of angle.
3. installation for fermenting according to claim 1, which is characterized in that the fixation support (3) is Ball Grid load
Body, material are polyvinyl chloride or polyethylene, and density is 0.9~1g/cm3, diameter is 15~25cm, is internally provided with several parallel
Round sheet adsorbing medium, the adsorbing medium diameter be 12~25cm, material be plant fiber or synthetic fibers.
4. installation for fermenting according to claim 3, which is characterized in that be transversely provided in the fermentation tank (1) several
The flase floor of different height, adjacent two pieces of flase floors are one group, between Ball Grid carrier prolonged by lontitudinal series by cord exist
It together, is 2~6cm with spacing between a string of Ball Grid carrier, the spacing between adjacent two string is 3~8cm.
5. installation for fermenting according to claim 4, which is characterized in that the vertical stirring device (2) include motor (21),
Flat paddle leaf (22) and pusher blade (23), the motor (21) is located at the top of fermentation tank (1), for driving flat paddle leaf
(22) and the rotation of pusher blade (23)；The flat paddle leaf (22) is located at the nearly top of fermentation tank (1)；The push type
Blade (23) is at the neutral gear between two adjacent groups flase floor.
6. installation for fermenting according to claim 1, which is characterized in that fermentation tank (1) ratio of height to diameter is 1.2~2:
1, pot bottom is circular cone type or oblique low-head.
7. installation for fermenting described in claim 1 prepares the application in ethyl alcohol in Immobilized yeast, which is characterized in that including
Following steps:
S1: fermentation strain is adsorbed in fixation support (3), is then filled into fermentation tank (1)；
S2: it is pumped into fermentation liquid into fermentation tank (1), is stirred, circulating fermentation；
S3: after fermentation, fermentation liquid is discharged, again the fermentation medium of stream plus hair.
8. application according to claim 7, which is characterized in that the fermentation strain is saccharomyces cerevisiae, active Angel Yeast
Powder or recombination zymomonas mobilis；The fermentation liquid is human configuration culture medium, corn powder saccharification liquid, cassava saccharified liquid, ageing
Any one or the combination of several of them in the saccharified liquid of rice, stalk hydrolyzate or molasses；Wherein, human configuration culture medium prescription
Are as follows: glucose 180-250g/L, peptone 2-5g/L, yeast extract 2-5g/L, pH4.0-5.0；The corn powder saccharification liquid, cassava
Saccharified liquid, be aged rice saccharified liquid material water quality ratio be 2.2~3:1；In the stalk hydrolyzate, expect after delignification
Liquid mass ratio is 1:2~6, pH 4.2-4.5；The initial total sugar of the molasses is 180-240g/L.
9. application according to claim 7, which is characterized in that the revolving speed 10-30r/min of the stirring.
10. application according to claim 7, which is characterized in that the flow of the circulation is 0.5-2BV/h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910372735.XA CN110117526A (en) | 2019-05-06 | 2019-05-06 | A kind of novel fermentation device and its application in ethyl alcohol is prepared in Immobilized yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910372735.XA CN110117526A (en) | 2019-05-06 | 2019-05-06 | A kind of novel fermentation device and its application in ethyl alcohol is prepared in Immobilized yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110117526A true CN110117526A (en) | 2019-08-13 |
Family
ID=67521796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910372735.XA Pending CN110117526A (en) | 2019-05-06 | 2019-05-06 | A kind of novel fermentation device and its application in ethyl alcohol is prepared in Immobilized yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110117526A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363650A (en) * | 2020-03-31 | 2020-07-03 | 浙江古越龙山绍兴酒股份有限公司 | Immobilized yeast fermentation tank |
| CN113957102A (en) * | 2021-08-23 | 2022-01-21 | 中粮生化能源（肇东）有限公司 | Method for continuously producing ethanol |
| CN117844610A (en) * | 2023-12-31 | 2024-04-09 | 枣庄市杰诺生物酶有限公司 | Preparation device and method of high-purity aminobutyric acid |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103436561A (en) * | 2013-09-05 | 2013-12-11 | 江南大学 | Method for fermentation production of succinic acid by using cotton fiber material to fix actinobacillus succinogenes |
| CN103865750A (en) * | 2012-12-13 | 2014-06-18 | 南京工业大学 | Ethanol production equipment coupling fixed bed fermentation and separation |
| WO2014089812A1 (en) * | 2012-12-13 | 2014-06-19 | 南京工业大学 | Method for producing ethanol by coupling immobilization bed fermentation with separation |
| CN105219809A (en) * | 2015-09-28 | 2016-01-06 | 浙江汇宁生物科技有限公司 | A kind of mobile fibre bed reactor and be applied to butyric acid produce method |
| CN106399422A (en) * | 2016-12-13 | 2017-02-15 | 南京高新工大生物技术研究院有限公司 | Preparation method of bacterial cellulose |
| CN106906253A (en) * | 2017-03-28 | 2017-06-30 | 南京工业大学 | A kind of method using surface immobilized technology fermenting and producing L lactic acid |
| CN108676788A (en) * | 2018-04-11 | 2018-10-19 | 南京高新工大生物技术研究院有限公司 | A kind of fixed yeast continuously ferments technology |
| CN210215327U (en) * | 2019-05-06 | 2020-03-31 | 南京高新工大生物技术研究院有限公司 | Device for preparing ethanol by fermentation of immobilized yeast |
-
2019
- 2019-05-06 CN CN201910372735.XA patent/CN110117526A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865750A (en) * | 2012-12-13 | 2014-06-18 | 南京工业大学 | Ethanol production equipment coupling fixed bed fermentation and separation |
| WO2014089812A1 (en) * | 2012-12-13 | 2014-06-19 | 南京工业大学 | Method for producing ethanol by coupling immobilization bed fermentation with separation |
| CN103436561A (en) * | 2013-09-05 | 2013-12-11 | 江南大学 | Method for fermentation production of succinic acid by using cotton fiber material to fix actinobacillus succinogenes |
| CN105219809A (en) * | 2015-09-28 | 2016-01-06 | 浙江汇宁生物科技有限公司 | A kind of mobile fibre bed reactor and be applied to butyric acid produce method |
| CN106399422A (en) * | 2016-12-13 | 2017-02-15 | 南京高新工大生物技术研究院有限公司 | Preparation method of bacterial cellulose |
| CN106906253A (en) * | 2017-03-28 | 2017-06-30 | 南京工业大学 | A kind of method using surface immobilized technology fermenting and producing L lactic acid |
| CN108676788A (en) * | 2018-04-11 | 2018-10-19 | 南京高新工大生物技术研究院有限公司 | A kind of fixed yeast continuously ferments technology |
| CN210215327U (en) * | 2019-05-06 | 2020-03-31 | 南京高新工大生物技术研究院有限公司 | Device for preparing ethanol by fermentation of immobilized yeast |
Non-Patent Citations (1)
| Title |
|---|
| 虞炳钧, 郑裕国, 张福明, 王普: "膜片状填料固定化酵母薯干酒精发酵生物反应器的研究", 工业微生物, vol. 25, no. 03, pages 24 - 28 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363650A (en) * | 2020-03-31 | 2020-07-03 | 浙江古越龙山绍兴酒股份有限公司 | Immobilized yeast fermentation tank |
| CN113957102A (en) * | 2021-08-23 | 2022-01-21 | 中粮生化能源（肇东）有限公司 | Method for continuously producing ethanol |
| CN117844610A (en) * | 2023-12-31 | 2024-04-09 | 枣庄市杰诺生物酶有限公司 | Preparation device and method of high-purity aminobutyric acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110117526A (en) | A kind of novel fermentation device and its application in ethyl alcohol is prepared in Immobilized yeast | |
| US4413058A (en) | Continuous production of ethanol by use of flocculent zymomonas mobilis | |
| CN101235391B (en) | Method and system for continuously separating ethanol and utilizing residual heat based on solid-state fermentation material | |
| CN103243023B (en) | Circulating synchronous straw enzymatic hydrolysis fermentation fuel ethanol reactor and reaction method thereof | |
| CN108660132A (en) | A kind of surface immobilized yeast fermentation technique | |
| CN103333787B (en) | Packing tower type continuous surface fermentation apparatus and implementation method thereof | |
| CN108676788A (en) | A kind of fixed yeast continuously ferments technology | |
| CN102321537B (en) | Bubbling bioreactor used for plant tissue culture | |
| CN104946516A (en) | Device and method for producing butyl alcohol through continuous fermentation of lignocellulose | |
| CN109988757A (en) | A kind of method that surface immobilized yeast semicontinuous fermentation prepares ethyl alcohol | |
| Liu et al. | Bioethanol production from potato starch by a novel vertical mass-flow type bioreactor with a co-cultured-cell strategy | |
| Ariyajaroenwong et al. | Capability of sweet sorghum stalks as supporting materials for yeast immobilization to produce ethanol under various fermentation processes | |
| CN108441416A (en) | A kind of static fermentation device and its fermentation process and application | |
| CN210215327U (en) | Device for preparing ethanol by fermentation of immobilized yeast | |
| CN109943555A (en) | A method of distiller's yeast, which is continuously prepared, using surface immobilized technology carries out alcoholic fermentation | |
| CN203247263U (en) | Straw synchronized enzymolysis and fermentation fuel alcohol circulation reactor | |
| Love et al. | Continuous ethanol fermentation at 45 C using Kluyveromyces marxianus IMB3 immobilized in calcium alginate and kissiris | |
| CN102897909A (en) | Self-circulation anaerobic reactor | |
| CN202830013U (en) | Fermentation device used for producing alcohol | |
| CN110117588A (en) | A kind of Novel fermentation tank and its application in ethyl alcohol is prepared in Immobilized yeast | |
| JP5249106B2 (en) | Method for continuous fermentation production of ethanol | |
| CN106867920A (en) | A kind of saccharomyces cerevisiae and application thereof | |
| Parekh et al. | Performance of a novel continuous dynamic immobilized cell bioreactor in ethanolic fermentation | |
| CN107487843B (en) | Vacuum solar heat collection type outdoor upflow anaerobic sludge bed reactor | |
| CN216303775U (en) | Fermentation tank and system for producing ethanol through fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |