CN110115645A - Multi path artificial nerve trachea and preparation method thereof - Google Patents
Multi path artificial nerve trachea and preparation method thereof Download PDFInfo
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- CN110115645A CN110115645A CN201910312750.5A CN201910312750A CN110115645A CN 110115645 A CN110115645 A CN 110115645A CN 201910312750 A CN201910312750 A CN 201910312750A CN 110115645 A CN110115645 A CN 110115645A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/222—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2240/001—Designing or manufacturing processes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction
Abstract
The present invention relates to multi path artificial nerve trachea and preparation method thereof, the bridge joint that can be used for customizing repairs that neurologic defect position is remote, proximal end.It is disclosed by the invention to customize the multi path artificial nerve trachea for imitating the natural anatomical structure of nerve, it mainly include to imitate the natural multichannel bracket (nerve trachea tube body) of anatomical structure of nerve and the biofilm structure (nerve trachea tube wall) of its outer layer covers.What tube body multi-channel structure can customize organizes the formation of better matching relationship with the nerve tract of different zones and size in nerve to be repaired, by guiding the specific nerve tract tissue in neurologic defect position proximal end to reach distal end in different customization channel designs, realize that reduce nerve tract dissipates growth during the growth process, be interweaved phenomenon, advantageously reduces the mismatch rate of proximal end new life nerve tract tissue and distal end target area.
Description
Technical field
The present invention relates to nerve trachea fields, are a kind of bionical multichannels of customization for imitating the natural anatomical structure of nerve
Artificial nerve catheter designs preparation method.
Background technique
Peripheral nerve injury is a kind of common clinical problem, it is often caused by serious damage, so as to cause nerve
Defect.For being more than the defect of 4mm, simple direct sewing method cannot be taken, and need to defect proximally and distally between mention
It is bridged for graft.Nerve autograft be currently repair neurologic defect " goldstandard ", but its frequently result in donor site damage and
Functional rehabilitation is imperfect.
Nerve trachea has been developed that into a kind of method that neurologic defect is repaired in substitution autotransplantation, and structure only limits at present
In simple single channel or with the multi-channel structure of regular matrix.However, the natural anatomical structure of nerve tract is not this letter
Single single channel lumen or regular matrix structure, the size and number of nerve tract have obvious between different plant species, Different Individual
Difference, its arrangement mode is all not all the same in its different cross section for even same nerve.
Therefore, nerve trachea structure at this stage is only limitted to the multichannel nerve conduit of single channel or regular matrix, cannot
Arrangement and distribution of the good analog neuron beam in nerve can not really restore the dry middle multiple groups nerve tract of original nerve three
Distribution and rule out of shape on dimension space, above-mentioned nerve trachea structure are not applied to the best of recovery peripheral nerve defection
Selection.
Summary of the invention
The object of the present invention is to provide a kind of multi path artificial nerve trachea, customizes and imitates the natural anatomical structure of nerve,
It is internal with anatomy multiple groups channel design similar with target nerve to be repaired, being capable of axially directed nerve tract arrival
Distal end dominates area, and neurologic defect position is matched good isolated internal channel and be bridged with a plurality of.
Another object of the present invention is to provide a kind of methods for preparing above-mentioned multi path artificial nerve trachea.
Multi path artificial nerve trachea of the present invention mainly includes nerve trachea tube body, is equipped in nerve trachea tube body multiple
Imitate the channel of the natural anatomical structure of nerve, the outer layer covers nerve trachea tube wall of nerve trachea tube body, nerve trachea tube wall
For biofilm structure, it is used to analog neuron adventitial tissue.
Nerve trachea tube body and nerve trachea tube wall, are formed by one or more Material claddings, and have be interconnected
Porous structure, conducive to carrying out between nerve trachea tubular body and outside, nutriment is exchanged and waste is discharged.It is understood that
It is that nerve trachea tube body is equipped with porous structure, porous structure is again provided on nerve trachea tube wall, due to nerve trachea pipe
Wall wraps up nerve trachea tube body, then the two is interconnected by porous structure.
Number of channels be it is multiple, each access needle to different parts target nerve beam design different contours profiles etc. ginseng
Number, multichannel lumen diameter is usually 100-2000 μm.
The multi-channel structure being arranged in nerve trachea tube body is with the anatomy of nerve tract each in target nerve to be repaired
Parameter is foundation, the single nerve tract of single channel priority match in multi-channel structure, when single channel need to match more nerve tracts
When, the multiple groups nerve tract of closely located or target organ structure, function identical (or similar) is preferentially integrated into a channel.
In multi path artificial nerve trachea implantation process, the lumen in multiple channels in nerve trachea tube body can with it is to be repaired
Multiple neurologic defect both ends nerve tract organizes the formation of matched well relationship.
Further, multi-channel structure, which is provided, forms beam group with the consistent guidance in original nerve beam direction out of shape, help proximal end
Nerve fiber docked with the corresponding target area in distal end, passing through, which reduces mismatch rate, promotes neurological functional recovery.
The both ends of nerve trachea tube wall are longer than the both ends of nerve trachea tube body, and length is longer than tube body both ends 1-3mm, use
It is acted in providing with perilemma epineurium bridge joint, nerve trachea pipe thickness is 100-400 μm.
Nerve trachea tube wall provides protection for repair process, specifically:
When axial drawing of the nerve fiber by some strength, nerve trachea tube wall passes through pulls and extends
Dispersion drawing stress, when axial stress is more than that tube wall extends the limit, fracture occurs for tube wall to avoid nerve fiber by excess load
Drawing and be further damaged.
Outer wall and internal layer tube body have synergistic mechanism during neural restoration, and tube wall and tube body are presented fast, slow two
Kind degradation rate.The premature degradation of tube wall in vivo can raise regeneration chamber periphery and generate (the cleaning of more macrophages
Effect), clean regenerative environ-ment is provided for newborn nerve fiber.The slow degradation process of tube body can provide sufficient space size and
The nerve regneration channel of support strength is the powerful guarantee of the intracavitary good blood fortune circulation of tube channel, discharge nerve can be assisted disconnected
The cell and metabolic waste of surface damage.
The multi path artificial nerve trachea of customization can drive nerve tract to be formed in the multichannel of customization and original mind
Through similar multiple channel (beam group) structures of trunk, the long axis direction and nerve tract in each channel are out of shape consistent in neural trunk,
Newborn nerve fiber in channel, meets the out of shape and regularity of distribution of the nerve tract in nerve naturally, and directional guide proximal end is newborn
The corresponding target area in the distal end nerve tract Zhi Peizhi, to improve neural restoration accuracy.By by the above-mentioned nerve regneration channel with
Matched to neural broken ends of fractured bone section nerve tract tissue, can reduce newborn nerve tract be interweaved in nerve graft, nothing
The case where sequence is grown, advantageously reduces the mismatch rate of proximal end new life nerve tract tissue and distal end target area.
The present invention compared with the existing technology the advantages of be nerve trachea tube body multi-channel structure have and the neural broken ends of fractured bone cut
The similar engineering anatomic morphological of the nerve tract in face.Channel and nerve tract can form preferable matching relationship, thus by one group or
Multiple groups structure, the similar nerve tract of function and source are limited in specific nerve regneration channel, pass through nerve trachea tube wall
The growth of the unordered divergence expression of physical centralization effect limitation nerve, improves the neural axon quantity in unit area, drives new life
Nerve in channel formed beam group.Further, nerve tract is out of shape in the multichannel lumen long axis and original nerve tissue of conduit
Unanimously, the neural directional guide of above-mentioned Shu Zuhua to the corresponding target area in distal end can be docked.Its advantages are by reducing mind
Neurological functional recovery effect is improved through mispairing incidence.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will to embodiment or
Attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
The embodiment of the present invention for those of ordinary skill in the art without creative efforts, can be with
Other attached drawings are obtained according to the attached drawing of offer.
Fig. 1 is to intend bionical new zealand white rabbit sciatic nerve cross section tissue slice map.
Fig. 2 is the present invention 4 channel artificial nerve catheter lumen section views of design, the nerve in channel and target nerve
The matching relationship of beam.
Fig. 3 is to prepare CANC process using die methods, is illustrated: 1. duct forming molds, 2. conduits are socketed mold,
3. thimble, 4.15% (w/v) gelatin solution, 5. tube bodies (multichannel bracket), 6. tube walls (PLGA biomembrane), 7.CANC conduit.
Fig. 4 is CANC structure observation under scanning electron microscope, observes catheter outer PLGA under A. scanning electron microscope
Biomembrane (140 times of amplification), rectangular box enlarged drawing (corresponding B, amplify 500 times) display PLGA fibre diameter and pore structure,
C is multi-channel structure (amplification 20 times) of the observation using 4 passage catheters as representative, rectangular box amplification under scanning electron microscope
Figure (corresponding D, amplify 200 times) display gelatin pore structure.
Fig. 5 is Fluorescence Retrograde Tracing detection, A. new zealand white rabbit sciatic nerve trunk (S) and its nervus tibialis branch
(T), nervus peroneus communis branch (P), B. Fluorescence Retrograde Tracing detection ideograph.(a, b) is that tracer drives in the wrong direction through nerve tract respectively
The simulation drawing out of shape in single channel conduit (1-CANC) or multichannel (4-CANC), quilt C.1-CANC and in 4-CANC reparation group
The neuronal quantity situation of NY, FB, FB-NY label, FB-NY is by Fluorescence Retrograde mark D.1-CANC and in 4-CANC reparation group
Remember the percentage of neuron.
Fig. 6 is the neural tissue slice result and analysis repaired using 1-CANC and 4-CANC, A. Toluidine blue staining method
And two kinds of conduits of 1-CANC, 4-CANC are observed under transmission electron microscope and repair nerve slice as a result, B. analysis more above-mentioned two
Difference of the nerve trachea of kind in terms of regenerated nervous fibers quantity, density, diameter and fiber myelin wall thickness.
Fig. 7 is each conduit cavity internal diameter parameter, and data show that all conduit cavity cross-sectional areas are almost the same in table,
Match index (MI) shows that the matching degree of nerve trachea channel and beam, 4-CANC and target nerve matching are higher than 1-CANC.
Specific embodiment
Embodiment 1
Intend bionical new zealand white rabbit sciatic nerve tissues slice according to shown in FIG. 1, it is more that customization designs one kind
Channel artificial nerve catheter, the multi path artificial nerve trachea include nerve trachea tube body, and it is logical to be equipped with 4 in nerve trachea tube body
Road, then the multi path artificial nerve trachea is 4 channel nerve tracheas (4-CANC), is designed using computer aided design software
Conduit cross section is as shown in the figure for being located at right side in Fig. 2.
Nerve trachea tube body is ellipsoidal structure, and nerve trachea tube length 10mm, the long axis of nerve trachea tube body is
4mm, the short axle of nerve trachea tube body are 2.5mm.
It is equipped with 4 channels in nerve trachea tube body, is respectively defined as 1# lumen described in figure channel, 2# lumen channel, 3#
Lumen channel, 4# lumen channel
1# lumen channel diameter is 1.2mm, and 2# lumen channel diameter is 0.7mm, and 3# lumen channel diameter is 0.7mm, 4#
Lumen channel diameter is 0.6mm.
Lumen perimeter 10.05mm, lumen gross area 2.18mm2, match index (MI) 84.4% (table 1).
It compares with 4 channel nerve tracheas (4-CANC), the figure in Fig. 2 positioned at left side provides a kind of 1 channel mind
Through conduit (1-CANC), 1# lumen channel diameter is 1.7mm, match index (MI) 75.5% (table 1).
Multi path artificial nerve trachea (CANC) with above structure is prepared by means of mold shown in Fig. 3,
Above-mentioned data file importing printer is prepared into mold, specific as follows:
One, prepared by nerve trachea tube body (multichannel bracket)
1. gelatin particle is dissolved in deionized water, configuration concentration is 15% (w/v) gelatin solution.
2. pouring gelatin solution to socket mold (2) and correspondingly configured mold (1), required successively according to channel diameter
It is inserted into the thimble (3) of respective diameters, freezing solid is carried out in -80 DEG C of refrigerators.
As shown in figure 3, socket mold (2) has both ends open, it is respectively embedded into molding die (1) in both ends open, up and down
There is the cavity for accommodating gelatin solution between two molding dies (1), be then inserted into respectively by two molding dies (1) more
The purpose of a thimble (3), insertion thimble (3) is to produce the intracorporal channel of nerve trachea pipe, carry out in -80 DEG C of refrigerators
Freeze solid.
3. rapidly removal both mold ends fixation after, remove thimble (3), release gelatin conduit, be placed in be pre-chilled it is cold
It is lyophilized 48 hours in lyophilizer.
It after cryofixation, removes thimble (3), the molding die (1) at removal socket mold (2) both ends is then solid by freezing
The gelatin conduit of shape is released, and is placed in the freeze drier being pre-chilled and is lyophilized 48 hours.
4. freeze-drying forming processing: the parameter of setting being lyophilized in freeze drier are as follows: -30 DEG C, 0.05mPa continuous freeze-drying 12
Hour, it is warming up to 0 DEG C of holding 6h under vacuum state, is warming up to 22 DEG C of holding 30-60min, vacuum is released and rises to room temperature, obtain
Uncrosslinked nerve trachea tube body.
5. cross-linking process: be placed in N- (3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC, it is molten
Spend 0.12mol/L) and N-hydroxysuccinimide (NHS, solubility 0.06mol/L) mixed solution in, be crosslinked at 4 DEG C
Reaction 12 hours obtains multichannel nerve conduit tube body (5).
Two, prepared by nerve trachea tube wall (PLGA biomembrane):
1. PLGA particle (LA:GA=75:25, molecular weight 100000) is dissolved in dichloromethane solution, being made into concentration is
The PLGA solution of 10% (w/v).
2. PLGA solution is transferred in the 10ml glass syringe of electrostatic spinning machine and is connected with Teflon pipe.
3. setting distance 15cm between the spinning syringe needle and receiver board of electrostatic spinning agent, the horizontal movement velocity of receiver board
For 0.00147mm/s, the injection rate for adjusting syringe is 3mL/h, and adjusting electrostatic spinning machine output voltage is 13-15kV, even
Continuous spinning 1h, obtains PLGA biomembrane (6), as nerve trachea tube wall.
The mode of nerve trachea tube body and the synthesis of nerve trachea tube wall are as follows: the PLGA biomembrane (6) of acquisition is placed in 40 DEG C
Vacuum drying oven in dry after with a small amount of gelatin solution the PLGA biomembrane (6) of acquisition is fixed on nerve trachea tube body (5)
Surface obtains CANC (7) and observes (Fig. 3) under a scanning electron microscope.
Above-mentioned CANC (7) are subjected to animal surgery and repairing effect assessment, specifically, the CANC (7) of acquisition is passed through
Co60New zealand white rabbit Sciatic (10mm) model is implanted into after illumination-based disinfection sterilizing.It performs the operation under special microscope in 8X
Remote, the Proximal beam arrangement architecture (Fig. 2) according to neurologic defect, after catheter channel is matched with nerve tract, by PLGA biomembrane
(6) neurologic defect both ends perilemma epineurium structure is fixed on micro- dedicated suture.Postoperative conventinal breeding is adopted in given time point
With Fluorescence Retrograde Evaluation of detection methods nerve regneration accuracy (Fig. 5), nerve regneration quality and quantity then uses micro- sem observation
Neurohistology dicing method assesses (Fig. 6).
Comparative example 1
1 channel nerve trachea of design is customized according to new zealand white rabbit sciatic nerve tissues slice result (Fig. 1)
(1-CANC) designs conduit cross section (Fig. 2) using computer aided design software, and conduit is ellipsoidal structure, catheter length
10mm, conduit long axis are 4mm, and short axle 2.5mm, 1# lumen channel diameter is 1.7mm, lumen perimeter 5.34mm, the lumen gross area
2.27mm2, match index (MI) 77.1%.Remaining step is the same as embodiment 1.
Experimental result is shown:
Regenerate accuracy evaluation: 4-CANC and 1-CANC have same population fluorescent marker neuron (FB-, NY-,
FB-NY the neuron (FB-NY) of mispairing), and in 4-CANC occurs relative to 1-CANC negligible amounts (Fig. 5).Regenerating nerve is fine
Dimension amount, quality evaluation: 4-CANC and 1-CANC is all the same in terms of the sum and density of newborn medullated fibers, and
In terms of medullated fibers diameter and its myelin wall thickness, 4-CANC is better than 1-CANC (Fig. 6).
It is grown in conclusion embodiment limits nerve tract divergence expression in lumen relative to comparative example, reduces nerve
Mismatch rate, and show the advantage that medullated fibers is reached maturity that can promote.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (9)
1. a kind of multi path artificial nerve trachea, which is characterized in that including nerve trachea tube body, outside the nerve trachea tube body
Layer package nerve trachea tube wall；Multiple channels are equipped in nerve trachea tube body；The nerve trachea tube body and the nerve trachea
Tube wall is formed by one or more Material claddings, and has interconnected porous structure.
2. multi path artificial nerve trachea according to claim 1, which is characterized in that nerve trachea tube wall both ends
Length is longer than the nerve trachea tube body.
3. a kind of preparation method of multi path artificial nerve trachea, which is characterized in that multi path artificial nerve trachea includes nerve
Catheter tube, the outer layer covers nerve trachea tube wall of the nerve trachea tube body；Nerve trachea tube body the preparation method comprises the following steps: step
One, gelatin particle is dissolved in deionized water, the gelatin solution that configuration concentration is 15%；Step 2 pours gelatin solution to mould
Tool, freezing solid is carried out in -80 DEG C of refrigerators, obtains gelatin conduit；Gelatin conduit is lyophilized and shapes, do not handed over by step 3
The nerve trachea tube body of connection；Uncrosslinked nerve trachea tube body and mixed solution are carried out cross-linking reaction, obtain nerve by step 4
Catheter tube.
4. the preparation method of multi path artificial nerve trachea according to claim 3, which is characterized in that mold includes both ends
The socket mold of opening, both ends open are respectively embedded into molding die, have between upper and lower two molding dies and accommodate gelatin solution
Cavity.
5. the preparation method of multi path artificial nerve trachea according to claim 4, which is characterized in that in step 2,
When gelatin solution is poured to mold, multiple thimbles are inserted by two molding dies respectively, are carried out in -80 DEG C of refrigerators cold
Freeze solid, obtains gelatin conduit.
6. the preparation method of multi path artificial nerve trachea according to claim 5, which is characterized in that in step 3,
Gelatin conduit is placed in the freeze drier being pre-chilled and is lyophilized 48 hours, obtains uncrosslinked nerve trachea tube body.
7. the preparation method of multi path artificial nerve trachea according to claim 6, which is characterized in that, will in step 4
Uncrosslinked nerve trachea tube body is placed in N- (3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-
In hydroxysuccinimide mixed solution, cross-linking reaction 12 hours at 4 DEG C obtain nerve trachea tube body.
8. the preparation method of multi path artificial nerve trachea according to claim 3, which is characterized in that nerve trachea tube wall
The preparation method comprises the following steps: step 5, by PLGA grain dissolution in dichloromethane solution, being made into concentration is 10%PLGA solution；Step 6,
PLGA solution is transferred in the 10ml glass syringe of electrostatic spinning machine and is connected with Teflon pipe, continuous spinning 1h is obtained
Nerve trachea tube wall.
9. the preparation method of multi path artificial nerve trachea according to claim 8, which is characterized in that nerve trachea tube body
With the mode of nerve trachea tube wall synthesis are as follows: the nerve trachea tube wall of acquisition is placed in 40 DEG C of vacuum drying oven after drying, is used
The nerve trachea tube wall of acquisition is fixed on nerve trachea tube surfaces by a small amount of gelatin solution.
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| CN110584829A (en) * | 2019-09-17 | 2019-12-20 | 深圳兰度生物材料有限公司 | Nerve repair catheter |
| CN110975008A (en) * | 2019-12-18 | 2020-04-10 | 武汉理工大学 | Preparation method of nerve repair drug delivery system with electrical stimulation and angiogenesis promotion effects |
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| CN2830409Y (en) * | 2004-05-18 | 2006-10-25 | 中山大学附属第一医院 | Tissue engineering ambient nerve graft |
| CN1593354A (en) * | 2004-06-25 | 2005-03-16 | 清华大学 | Nerve tissue engineering tube type bracket and method for making same |
| CN101084025A (en) * | 2004-09-14 | 2007-12-05 | 新加坡科技研究局 | Porous biomaterial-filler composite and a method for making the same |
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| US20120150205A1 (en) * | 2009-08-12 | 2012-06-14 | Snu R&Db Foundation | Silk nanofiber nerve conduit and method for producing thereof |
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| CN103648536A (en) * | 2011-04-05 | 2014-03-19 | 弗赖堡大学医院 | Biocompatible and biodegradable gradient layer system for regenerative medicine and for tissue support |
| CN104382672A (en) * | 2014-11-14 | 2015-03-04 | 东华大学 | Double-layer multichannel nerve conduit and preparation method thereof |
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| CN108977405A (en) * | 2018-08-09 | 2018-12-11 | 西南大学 | A kind of instant 3D cell growth bracket and preparation method thereof |
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