CN110106219A - A kind of method that solid culture medium residue of cordyceps militaris recycles - Google Patents
A kind of method that solid culture medium residue of cordyceps militaris recycles Download PDFInfo
- Publication number
- CN110106219A CN110106219A CN201910337200.9A CN201910337200A CN110106219A CN 110106219 A CN110106219 A CN 110106219A CN 201910337200 A CN201910337200 A CN 201910337200A CN 110106219 A CN110106219 A CN 110106219A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- residue
- cordyceps militaris
- solid culture
- medium residue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a kind of methods that solid culture medium residue of cordyceps militaris recycles, and are related to solid culture medium residue of cordyceps militaris reutilization technology field.Solid culture medium residue of cordyceps militaris through heated-air drying is crushed, sieving processing；First be dissolved in water residue dry powder, adjusts the temperature to 50 DEG C, after pH is 10, passes sequentially through 20/28kHz sequence double frequency counter current ultrasonic wave device reaction cavity in a circulating manner and carry out ultrasonic pretreatment；PH is adjusted to 6.5 again, alpha-amylase is added to be digested；Enzymolysis liquid pH is finally recalled to 10, adds alkali protease to be digested with 6000U/g (residue silty amount) enzyme concentration, obtains the zymolyte with antihypertensive function.Utilize ultrasonic wave added enzyme treatment technique, using remaining solid culture medium residue after gathering in fructification as raw material, the preparation for carrying out high added value blood pressure-lowering functional factor, can not only make full use of Cordyceps militaris resource, additionally it is possible to effectively solve the problems, such as that Cordyceps militaris cultivates the waste that industry generates.
Description
Technical field:
The present invention relates to solid culture medium residue of cordyceps militaris reutilization technologies, specifically using the side of ultrasonic wave added enzymatic hydrolysis
Method extracts blood pressure-lowering functional factor from solid culture medium residue of cordyceps militaris, belongs to field of biotechnology.
Background technique:
Cordyceps militaris also known as Cordceps militaris are Cordyceps sinensis fungus, do not have strict requirements for host and living environment, be mesh
The preceding cordyceps sinensis that uniquely can be realized artificial culture.Since the medicine of Cordyceps militaris, nourishing effects are more brilliant, new money is had been cited as
Source food, application prospect are very wide.Though it is with considerable scale for the production of Tissue Culture of Cordyceps militaris fructification, in harvest
When entity, remaining a large amount of Chinese caterpillar fungus culture medium residues are discarded, and not only can cause environmental pollution；It is rich in residue
Cordyceps sinensis polysaccharide, starch sugar and protein matter, also result in the wasting of resources.The method recycled for Chinese caterpillar fungus culture medium residue
As current research hot spot, environmental problem not only can solve, can also improve the economic profit of enterprise.
Correlative study discovery, Cordyceps Militaris in order to preferably invade in pin main body and efficiently use the nutrition of host at
Point, a variety of biological enzymes can be secreted into culture medium, so that some macro-nutrients in culture medium be digested.Yang Xue exists
In " influence of the ultrasonic pretreatment to rice protein enzymolysis product ACE inhibiting rate " text, research finds the enzymatic hydrolysis of rice protein
Product has good hypotensive activity；The patent of invention that China Patent No. is 201810189732.8, disclosing has well
The isolation and purification method of the silkworm pupa protein polypeptide of ACE inhibitory activity；Peng Lei etc. is in " ultrasound pretreatment enzyme process preparation garlic decompression function
In the research of a energy factor " text, research finds that the hypopiesin (par-tial polysaccharide) of non-fully polypeptide also has hypotensive activity.
Currently, rest on the extraction of the single components such as Cordyceps sinensis polysaccharide, cordycepin mostly for the research of solid culture medium residue of cordyceps militaris,
And the research of short time enzymatic hydrolysis exploitation culture based draff hypotensive activity is combined to yet there are no using the metabolism of Cordyceps Militaris
Report.
Ultrasound-assisted enzymolysis technology, because it can overcome reaction efficiency, Product Activity and product in traditional zymolysis technique
Deficiencies, the advantages such as its action time is short in addition, easy to operate, easy to control, temperature rise is low such as yield is low are widely used in enzymatic hydrolysis
Investigation on intensification.Zhang Yanyan " is being ground based on the pretreated gluten protein ace inhibitory peptide preparation of ultrasound and its process in-situ monitoring technology
Study carefully " one it the article pointed out that enzymolysis property can be improved by pre-processing using suitable ultrasound works mode to raw material, it improves enzymatic hydrolysis and produces
The hypotensive activity of object.But ultrasonic washing trough, ultrasonic cell disintegration instrument are rested on mostly for the research of ultrasonic technology at present
Etc. equipment, can not change ultrasound operating mode.In addition ultrasonic wave added zymolysis technique prepares solid culture medium residue of cordyceps militaris
Relevant research also has not been reported.
According to the characteristic of solid culture medium residue of cordyceps militaris, using ultrasonic wave added enzyme treatment technique, after gathering in fructification
Remaining solid culture medium residue is raw material, carries out the preparation of high added value blood pressure-lowering functional factor, can not only effectively solve
Cordyceps militaris cultivates the waste problem that industry generates, moreover it is possible to make full use of Cordyceps militaris resource.
Summary of the invention
It is an object of the invention to overcome the shortcomings of the above-mentioned extraction of tradition in the prior art or enzyme solution, pupa is made full use of
Cordyceps sinensis solid culture medium residue characteristic provides a kind of novel ultrasonic auxiliary preparation solid culture medium residue of cordyceps militaris antihypertensive function
The method of the factor.The present invention utilizes ultrasound-assisted enzymolysis technology, is to gather in remaining solid culture medium residue after fructification
Raw material carries out the preparation of high added value blood pressure-lowering functional factor, can not only make full use of Cordyceps militaris resource, additionally it is possible to effectively solve
Cordyceps militaris cultivates the waste problem that industry generates.
For method of the invention, it is specific the technical solution adopted is as follows:
A kind of method that solid culture medium residue of cordyceps militaris recycles, as steps described below:
Step 1, the crushing of culture medium residue: the solid culture medium residue of cordyceps militaris through heated-air drying is crushed, at sieving
Reason；
Step 2, the pretreatment of culture medium residue: into culture medium residue dry powder plus water obtains suspension, adjusts the temperature to
50 DEG C, pH be 10 after, by setting ultrasound mode passed sequentially through in a manner of circulated in countercurrent ultrasonic equipment reaction chamber carry out ultrasound
Processing, obtains pretreatment fluid；
Step 3, the amylase enzymolysis of culture medium residue: being adjusted to 6.5 for the pH of pretreatment fluid, with 50.1U/g (residue powder
Quality) enzyme concentration adds amylase to be digested, obtain amylase enzymolysis liquid；
The protease hydrolyzed of culture medium residue: the pH of amylase enzymolysis liquid is recalled to 10, with 6000U/g (residue by step 4
Silty amount) enzyme concentration adds alkali protease to be digested, after zymolyte enzyme deactivation, through 4000rad/min, centrifugation 15min, must have
The zymolyte of antihypertensive function.
Further, in step 1, the solid culture medium residue is remaining solid culture after harvesting fruiting bodies of cordyceps militaris
Base is rich in mycelia and Cordyceps Militaris metabolite；The primary raw material of the solid medium is rice and silkworm chrysalis；The sieving processing
It was 60 meshes, and can effectively screens out useless residue and impurity.
Further, in step 2, the residue dry powder turbid liquid concentration is 10~50g/L.
Further, in step 2, the ultrasonic equipment is the ultrasonic wave work using 20/28kHz sequence double frequency adverse current
Operation mode, ultrasonic power density are 32~160W/L, and pretreatment time is 30min in reaction chamber.
Further, in step 3, the amylase is alpha-amylase；The amylase enzymolysis is that temperature is constant 60
DEG C, pH 6.5, digest 20min, can guarantee that starch quickly moderately digests in material, sufficiently discharge protein and Cordyceps sinensis polysaccharide.
Further, in step 4, the protease is alkali protease；The protease hydrolyzed, be temperature it is constant
In 60 DEG C, pH 10,40min is digested, can guarantee that proteolysis obtains the polypeptide with good ACE inhibitory activity in material.
The present invention carries out solid culture medium residue of cordyceps militaris blood pressure-lowering functional factor using the method for ultrasonic wave added enzymatic hydrolysis
Preparation, ultrasound pretreatment shorten enzymolysis time, improve hydrolysis result, improve the activity and yield of enzymolysis product.
Detailed description of the invention
Fig. 1 is 20/28kHz sequence double frequency counter current ultrasonic wave equipment schematic diagram；
In figure: 1 being supersonic generator, 2 be 28kHz ultrasound reactor, 3 be 20kHz ultrasound reactor, 4 to follow
Ring pump, 5 are water-bath.
Specific embodiment
Technical solution of the present invention is described in further detail below with reference to the drawings and specific embodiments.The present invention
There is versatility to other culture mediums rich in polysaccharide, albumen.
In hypertension Forming Mechanism, angiotensin converting enzyme (ACE) plays a crucial role during blood pressure control, and
And the appraisement system of vitro detection index ACE inhibiting rate is highly developed, therefore selects the ACE inhibiting rate evaluation of enzymatic hydrolysis final product
Its hypotensive activity.The measurement of enzymolysis product inhibitory activity is according to document " Huang L, Ma H, Li Y, et
al.Antihypertensive activity of recombinant peptide IYPR expressed in
Escherichia coli as inclusion bodies.[J].Protein Expression&Purification,
2012,83 (1): the method for 15-20. " carries out, with N- [3- (2- furyl) acryloyl]-L- phenylalanyl glycylglycine
(FAPGG) substrate as ACE catalysis, is studied the variation of its absorbance, is measured using enzyme labeling meter.
According to shown in Fig. 1,20/28kHz sequence double frequency counter current ultrasonic wave equipment in the present invention, structure includes ultrasonic wave hair
Raw device 1,28kHz ultrasound reactor 2,20kHz ultrasound reactor 3, circulating pump 4, water-bath 5.
The supersonic generator 1 is separately connected the left bottom of 28kHz ultrasound reactor 2,20kHz ultrasound through electric wire
The left bottom of wave reactor 3；The right hand top of the 28kHz ultrasound reactor 2 is connected to 20kHz ultrasonic wave through feed liquid pipe
The left bottom of reactor 3；The right hand top (treatment fluid import) of the 20kHz ultrasound reactor 3 is connected to through feed liquid pipe
The left side of circulating pump 4；The circulating pump 4 is connected to water-bath 5 through feed liquid pipe；The water-bath 5 is connected to through feed liquid pipe
The right side of circulating pump 4,28kHz ultrasound reactor 2 left bottom (treatment fluid outlet).Treatment fluid by circulating pump 4 by into
Material mouth, which circuits sequentially, carries out the pre- place of ultrasound in the reaction chamber for being pumped into 20kHz ultrasound reactor 3,28kHz ultrasound reactor 2
Reason, whole process can control ultrasonic power, the ultrasonic treatment total time of ultrasound reactor by supersonic generator 1.
Comparative example:
(1) solid culture medium residue of cordyceps militaris through heated-air drying is crushed, crosses 60 meshes, obtains residue dry powder；
(2) conventional enzymatic hydrolysis (no ultrasound pretreatment): the solid culture medium residue of cordyceps militaris that compound concentration is 30g/L suspends
Liquid, temperature regulating is 50 DEG C, pH maintains 10；50 DEG C of constant temperature stirring 30min are put into the thermostat water bath with rotor, are then adjusted
PH6.5, temperature 60 C are digested with 50.1U/g (residue silty amount) plus alpha-amylase, and pH6.5, temperature are kept in enzymolysis process
60 DEG C of degree adjusts pH to 10 rapidly after digesting 20min.
Then add alkali protease to be digested with 6000U/g (residue silty amount) enzyme concentration, is kept in enzymolysis process
PH10, temperature 60 C are centrifuged 15min at 100 DEG C after digesting 40min, collect supernatant under enzyme deactivation 10min, 4000rad/min,
Up to solid culture medium residue of cordyceps militaris blood pressure-lowering functional factor；
(3) Activity determination: being settled to 100mL after taking 80mL supernatant to adjust pH to 8.3, measure ACE inhibitory activity, be shown in Table
1。
Embodiment 1:
(1) solid culture medium residue of cordyceps militaris through heated-air drying is crushed, crosses 60 meshes, obtains residue dry powder；
(2) ultrasonic wave added digests: compound concentration is the solid culture medium residue of cordyceps militaris suspension of 30g/L, is heated to 50
It is ultrasonically treated after adjusting pH to 10 after DEG C.
For treatment fluid by reaction chamber in a manner of circulated in countercurrent, circulation revolution speed is 300rad/min.Ultrasonic treatment condition are as follows:
The power density that treatment fluid initial temperature is 50 DEG C, pH maintains 10, is ultrasonically treated is 32W/L, ultrasound mode is 20/28kHz suitable
Sequence double frequency adverse current ultrasound, sonication treatment time 30min.
It after pretreatment, is put into the thermostat water bath with rotor, adjusts pH6.5, temperature 60 C, then with 50.1U/g
(residue silty amount) enzyme concentration adds alpha-amylase to be digested, and pH6.5, temperature 60 C are kept in enzymolysis process, after digesting 20min
PH to 10 is adjusted rapidly.
Then add alkali protease to be digested with 6000U/g (residue silty amount) enzyme concentration, is kept in enzymolysis process
PH10, temperature 60 C are centrifuged 15min at 100 DEG C after digesting 40min, collect supernatant under enzyme deactivation 10min, 4000rad/min,
Up to solid culture medium residue of cordyceps militaris blood pressure-lowering functional factor.
(3) Activity determination: being settled to 100mL after taking 80mL supernatant to adjust pH to 8.3, measure ACE inhibitory activity, be shown in Table
1。
Embodiment 2:
(4) solid culture medium residue of cordyceps militaris through heated-air drying is crushed, crosses 60 meshes, obtains residue dry powder；
(5) ultrasonic wave added digests: compound concentration is the solid culture medium residue of cordyceps militaris suspension of 50g/L, is heated to 50
It is ultrasonically treated after adjusting pH to 10 after DEG C.
For treatment fluid by reaction chamber in a manner of circulated in countercurrent, circulation revolution speed is 300rad/min.Ultrasonic treatment condition are as follows:
The power density that treatment fluid initial temperature is 50 DEG C, pH maintains 10, is ultrasonically treated is 128W/L, ultrasound mode is 20/28kHz suitable
Sequence double frequency adverse current ultrasound, sonication treatment time 30min.
It after pretreatment, is put into the thermostat water bath with rotor, adjusts pH6.5, temperature 60 C, then with 50.1U/g
(residue silty amount) enzyme concentration adds alpha-amylase to be digested, and pH6.5, temperature 60 C are kept in enzymolysis process, after digesting 20min
PH to 10 is adjusted rapidly.
Then add alkali protease to be digested with 6000U/g (residue silty amount) enzyme concentration, is kept in enzymolysis process
PH10, temperature 60 C are centrifuged 15min at 100 DEG C after digesting 40min, collect supernatant under enzyme deactivation 10min, 4000rad/min,
Up to solid culture medium residue of cordyceps militaris blood pressure-lowering functional factor.
(6) Activity determination: being settled to 100mL after taking 80mL supernatant to adjust pH to 8.3, measure ACE inhibitory activity, be shown in Table
1。
Embodiment 3:
(1) solid culture medium residue of cordyceps militaris through heated-air drying is crushed, crosses 60 meshes, obtains residue dry powder；
(2) ultrasonic wave added digests: compound concentration is the solid culture medium residue of cordyceps militaris suspension of 10g/L, it is heated to 50
It is ultrasonically treated after adjusting pH to 10 after DEG C.
For treatment fluid by reaction chamber in a manner of circulated in countercurrent, circulation revolution speed is 300rad/min.Ultrasonic treatment condition are as follows:
The power density that treatment fluid initial temperature is 50 DEG C, pH maintains 10, is ultrasonically treated is 160W/L, ultrasound mode is 20/28kHz suitable
Sequence double frequency adverse current ultrasound, sonication treatment time 30min.
It after pretreatment, is put into the thermostat water bath with rotor, adjusts pH6.5, temperature 60 C, then with 50.1U/g
(residue silty amount) enzyme concentration adds alpha-amylase to be digested, and pH6.5, temperature 60 C are kept in enzymolysis process, after digesting 20min
PH to 10 is adjusted rapidly.
Then add alkali protease to be digested with 6000U/g (residue silty amount) enzyme concentration, is kept in enzymolysis process
PH10, temperature 60 C are centrifuged 15min at 100 DEG C after digesting 40min, collect supernatant under enzyme deactivation 10min, 4000rad/min,
Up to solid culture medium residue of cordyceps militaris blood pressure-lowering functional factor.
(3) Activity determination: being settled to 100mL after taking 80mL supernatant to adjust pH to 8.3, measure ACE inhibitory activity, be shown in Table
1。
The result of the different embodiment ACE inhibitory activities of table 1
| Sample number into spectrum | ACE inhibiting rate (%) |
| Comparative example | 38.14 |
| Embodiment 1 | 44.40 |
| Embodiment 2 | 79.94 |
| Embodiment 3 | 54.82 |
It, can be with by embodiment result it is found that the solid culture medium residue of cordyceps militaris reuse method through the invention
The preparation of high added value blood pressure-lowering functional factor is carried out, this can not only make full use of Cordyceps militaris resource, additionally it is possible to effectively solution pupa
Cordyceps sinensis cultivates the waste problem that industry generates.
Claims (6)
1. a kind of method that solid culture medium residue of cordyceps militaris recycles, it is characterised in that as steps described below:
Step 1, the crushing of culture medium residue: the solid culture medium residue of cordyceps militaris through heated-air drying is crushed, sieving processing；
Step 2, the pretreatment of culture medium residue: into culture medium residue dry powder plus water obtains suspension, adjusts the temperature to 50
DEG C, pH be 10 after, by setting ultrasound mode passed sequentially through in a manner of circulated in countercurrent ultrasonic equipment reaction chamber carry out ultrasound at
Reason, obtains pretreatment fluid；
Step 3, the amylase enzymolysis of culture medium residue: being adjusted to 6.5 for the pH of pretreatment fluid, with 50.1U/g (residue silty amount)
Enzyme concentration adds amylase to be digested, and obtains amylase enzymolysis liquid；
Step 4, the protease hydrolyzed of culture medium residue: recalling to 10 for the pH of amylase enzymolysis liquid, with 6000U/g (residue silty
Amount) enzyme concentration adds alkali protease to be digested, and zymolyte is after enzyme deactivation, and through 4000rad/min, centrifugation 15min, obtaining has drop
The zymolyte of blood pressure function.
2. a kind of method that solid culture medium residue of cordyceps militaris recycles according to claim, it is characterised in that step 1
In, the solid culture medium residue is remaining solid medium after harvesting fruiting bodies of cordyceps militaris, is rich in mycelia and Cordyceps Militaris generation
Thank to product；The primary raw material of the solid medium is rice and silkworm chrysalis；The sieving processing was 60 meshes, can effectively sieve
Except useless residue and impurity.
3. a kind of method that solid culture medium residue of cordyceps militaris recycles according to claim, it is characterised in that step 2
In, the residue dry powder turbid liquid concentration is 10~50g/L.
4. a kind of method that solid culture medium residue of cordyceps militaris recycles according to claim, it is characterised in that step 2
In, the ultrasonic equipment is that, using 20/28kHz sequence double frequency counter current ultrasonic wave operating mode, ultrasonic power density is 32
~160W/L, pretreatment time is 30min in reaction chamber.
5. a kind of method that solid culture medium residue of cordyceps militaris recycles according to claim, it is characterised in that step 3
In, the amylase is alpha-amylase；The amylase enzymolysis is that temperature is constant in 60 DEG C, pH 6.5, digests 20min,
It can guarantee that starch quickly moderately digests in material, sufficiently discharge protein and Cordyceps sinensis polysaccharide.
6. a kind of method that solid culture medium residue of cordyceps militaris recycles according to claim, it is characterised in that step 4
In, the protease is alkali protease；The protease hydrolyzed is that temperature is constant and is 60 DEG C, pH 10, enzymatic hydrolysis
40min can guarantee that proteolysis obtains the polypeptide with good ACE inhibitory activity in material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910337200.9A CN110106219B (en) | 2019-04-25 | 2019-04-25 | Method for recycling residue of cordyceps militaris solid culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910337200.9A CN110106219B (en) | 2019-04-25 | 2019-04-25 | Method for recycling residue of cordyceps militaris solid culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110106219A true CN110106219A (en) | 2019-08-09 |
| CN110106219B CN110106219B (en) | 2023-07-18 |
Family
ID=67486657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910337200.9A Active CN110106219B (en) | 2019-04-25 | 2019-04-25 | Method for recycling residue of cordyceps militaris solid culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110106219B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113133951A (en) * | 2020-01-19 | 2021-07-20 | 克一股份有限公司 | Production method and application of rice protein extract extracted from cordyceps militaris fermentation product |
| CN114990183A (en) * | 2022-06-11 | 2022-09-02 | 江苏科技大学 | Method for preparing cordycepin and antihypertensive peptide based on rice cordyceps militaris sporocarp |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731666A (en) * | 2011-04-07 | 2012-10-17 | 大连工业大学 | Method for extracting polysaccharide from cordyceps militaris medium |
| CN103755825A (en) * | 2014-01-03 | 2014-04-30 | 南京晓庄学院 | Method for extracting cordyceps substrate polysaccharide from waste cordyceps militaris culture medium and application of extract |
| CN105309974A (en) * | 2015-10-19 | 2016-02-10 | 安徽农业大学 | Method for preparing cordyceps militaris peptide oral liquid |
| CN106810618A (en) * | 2017-01-06 | 2017-06-09 | 徐州鸿宇农业科技有限公司 | A kind of extraction from Chinese caterpillar fungus culture medium and the method for continuous polysaccharide enrichment |
| CN108084287A (en) * | 2017-10-26 | 2018-05-29 | 辽宁省农业科学院大连生物技术研究所 | The method that Cordyceps sinensis polysaccharide is extracted from solid culture medium residue of cordyceps militaris |
| CN108395467A (en) * | 2018-03-08 | 2018-08-14 | 浙江汇能生物股份有限公司 | The isolation and purification method of silkworm pupa protein polypeptide |
-
2019
- 2019-04-25 CN CN201910337200.9A patent/CN110106219B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731666A (en) * | 2011-04-07 | 2012-10-17 | 大连工业大学 | Method for extracting polysaccharide from cordyceps militaris medium |
| CN103755825A (en) * | 2014-01-03 | 2014-04-30 | 南京晓庄学院 | Method for extracting cordyceps substrate polysaccharide from waste cordyceps militaris culture medium and application of extract |
| CN105309974A (en) * | 2015-10-19 | 2016-02-10 | 安徽农业大学 | Method for preparing cordyceps militaris peptide oral liquid |
| CN106810618A (en) * | 2017-01-06 | 2017-06-09 | 徐州鸿宇农业科技有限公司 | A kind of extraction from Chinese caterpillar fungus culture medium and the method for continuous polysaccharide enrichment |
| CN108084287A (en) * | 2017-10-26 | 2018-05-29 | 辽宁省农业科学院大连生物技术研究所 | The method that Cordyceps sinensis polysaccharide is extracted from solid culture medium residue of cordyceps militaris |
| CN108395467A (en) * | 2018-03-08 | 2018-08-14 | 浙江汇能生物股份有限公司 | The isolation and purification method of silkworm pupa protein polypeptide |
Non-Patent Citations (13)
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113133951A (en) * | 2020-01-19 | 2021-07-20 | 克一股份有限公司 | Production method and application of rice protein extract extracted from cordyceps militaris fermentation product |
| CN113133951B (en) * | 2020-01-19 | 2023-06-09 | 克一股份有限公司 | Production method for extracting rice protein extract from cordyceps militaris fermentation product and application of rice protein extract |
| CN114990183A (en) * | 2022-06-11 | 2022-09-02 | 江苏科技大学 | Method for preparing cordycepin and antihypertensive peptide based on rice cordyceps militaris sporocarp |
| CN114990183B (en) * | 2022-06-11 | 2023-11-21 | 江苏科技大学 | Method for preparing cordycepin and antihypertensive peptide based on rice cordyceps militaris fruiting body |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110106219B (en) | 2023-07-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105560097B (en) | A kind of fresh peony flower ferment and its preparation method and application | |
| CN110106219A (en) | A kind of method that solid culture medium residue of cordyceps militaris recycles | |
| CN108342443A (en) | A kind of method that anti-oxidation peptide is extracted in the peony seeds dregs of rice | |
| CN101560260A (en) | Technique for extracting polysaccharide in glossy ganoderma mycelium cell and method thereof | |
| CN101560264A (en) | Process combination for extracting polysaccharide in golden mushroom mycelium cells and determination method thereof | |
| CN104855676B (en) | The method for preparing high sugar feed raw material using steam blasting | |
| CN105524966A (en) | Method for preparing ACE inhibitory peptides through bean pulp enzymolysis | |
| CN114128768A (en) | Rice bran oil body-curcumin mixed carrier and preparation method thereof | |
| CN104846046B (en) | A kind of method that gluten protein blood pressure lowering peptide is prepared based on the ultrasound-enhanced enzymolysis of order | |
| CN107095312A (en) | A kind of krill polypeptide formulations with reducing blood lipid ability and preparation method thereof | |
| CN106086138A (en) | A kind of method that microwave-assisted and membrane filtration prepare fresh water fin albumen oligopeptide | |
| CN104774894A (en) | Method for preparing gluten ACE inhibitory peptide through multi-mode ultrasonic wave strengthened enzymolysis | |
| Jin et al. | Improving Aspergillus niger seed preparation and citric acid production by morphology controlling-based semicontinuous cultivation | |
| CN104762355A (en) | Preparation method for fish scale collagen protein active peptide | |
| CN102952841A (en) | Method for preparing hyriopsis cumingii meat enzymolysis protein solution | |
| CN112890212A (en) | High-purity soybean dietary fiber and preparation method and application thereof | |
| CN109022519A (en) | The method of high-purity resistant starch is efficiently prepared under a kind of cryogenic conditions | |
| CN104845959A (en) | Method for preparing rapeseed polypeptide by using ultrasonic-assisted enzymolysis and immobilized alkali protease | |
| CN103981245A (en) | Method of preparing high-activity small peptide by utilizing spiral seaweed mud | |
| CN114634961A (en) | Preparation method of oyster oligomeric peptide powder with various biological activity functions | |
| CN106996925B (en) | Method for preparing blood sugar lowering peptide by using fluorescence spectrum monitoring enzyme method | |
| CN113046406A (en) | Preparation method of fishbone polypeptide | |
| CN105349607A (en) | Method for extracting mannatide efficiently | |
| CN106755243A (en) | A kind of preparation method of konjaku albumen source ACE inhibitor peptides | |
| CN105876072A (en) | Method for increasing solubility of rice protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |