CN110100735A - A kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud - Google Patents
A kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud Download PDFInfo
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- CN110100735A CN110100735A CN201910520121.1A CN201910520121A CN110100735A CN 110100735 A CN110100735 A CN 110100735A CN 201910520121 A CN201910520121 A CN 201910520121A CN 110100735 A CN110100735 A CN 110100735A
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- adventitious
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
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- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud, squamous subculture expands numerous a certain number of loud, high-pitched sound Tissue-cultured apple seedlings out, using loud, high-pitched sound Tissue-cultured apple seedling as starting material, inductive formation adventitious root；Using adventitious root as main experimental materials, after dedifferentiation formation callus is carried out in root induction bud culture medium, by breaking up generation adventitious bud again.Apple plants are obtained the beneficial effects of the invention are as follows efficient.
Description
Technical field
The invention belongs to agricultural technology fields, are related to a kind of side of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud
Method.
Background technique
For the tissue cultures mode of apple than wide, recent researches are also relatively more, the main Shoot Tip Culture including apple,
It apple embryo culture, endosperm culture, Anther Culture, Protoplast cuhnre and merges.But it can be sprouted using root induction,
It need further to study, root induction bud is not only a verifying to plant totipotency, moreover it can be used to the genetic transformation of apple
In system.
Currently, apple transgenic plant method is obtained mainly using the Agrobacterium tumefaciens mediated Apple Leaves that infect, and
Protoplast in Apple.Transgene efficiency is low, and cumbersome.Radical bud technology, only in some herbaceous plant, especially medicinal plant
Object is reported, but being rarely reported in xylophyta, and our experimental material is xylophyta apple, agrobacterium rhizogenes
It infects and only rests on the stage of taking root mostly, and we are from root that evoking adventive bud is significant again, this research may lose for apple
It passes conversion and new thinking and theoretical reference is provided.The adventitious root that known agrobacterium rhizogenes induces, high conversion efficiency, we will be sharp
It is sprouted with root induction, so that transgenic apples plant is obtained, to open up a kind of efficient Agrobacterium-mediated Genetic Transformation of Apple method.
Summary of the invention
The purpose of the present invention is to provide a kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud, this hairs
Bright beneficial effect is efficient acquisition apple plants.
The technical scheme adopted by the invention is that following the steps below:
Step 1: squamous subculture expands numerous a certain number of loud, high-pitched sound Tissue-cultured apple seedlings out, is starting material with loud, high-pitched sound Tissue-cultured apple seedling
Material, inductive formation adventitious root；
Step 2: using adventitious root as main experimental materials, carrying out dedifferentiation in root induction bud culture medium and form callus group
After knitting, by breaking up generation adventitious bud again.
Further, root induction bud culture medium prescription: MS+0.22mg/l TDZ+0.186mg/l NAA+30g sucrose+7g fine jade
Rouge.
Specific embodiment
The present invention is described in detail With reference to embodiment.
A kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud of the present invention follows the steps below:
Step 1: squamous subculture expands numerous ' loud, high-pitched sound ' Tissue-cultured apple seedlings a certain number of out, is with " loud, high-pitched sound " Tissue-cultured apple seedling
Beginning material, inductive formation adventitious root.
Step 2: using adventitious root as main experimental materials, carrying out dedifferentiation in root induction bud culture medium and form callus group
After knitting, by breaking up generation adventitious bud again.
Wherein root induction bud culture medium prescription: MS+0.22mg/l TDZ+0.186mg/l NAA+30g sucrose+7g agar
The above is only not to make limit in any form to the present invention to better embodiment of the invention
System, any simple modification that embodiment of above is made according to the technical essence of the invention, equivalent variations and modification,
Belong in the range of technical solution of the present invention.
Claims (2)
1. a kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud, it is characterised in that according to the following steps into
Row:
Step 1: squamous subculture expands numerous a certain number of loud, high-pitched sound Tissue-cultured apple seedlings out, using loud, high-pitched sound Tissue-cultured apple seedling as starting material,
Inductive formation adventitious root；
Step 2: using adventitious root as main experimental materials, carrying out dedifferentiation in root induction bud culture medium and form callus
Afterwards, by breaking up generation adventitious bud again.
2. according to a kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud described in claim 1, feature exists
In: the root induction bud culture medium prescription: MS+0.22mg/l TDZ+0.186mg/l NAA+30g sucrose+7g agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910520121.1A CN110100735A (en) | 2019-06-17 | 2019-06-17 | A kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910520121.1A CN110100735A (en) | 2019-06-17 | 2019-06-17 | A kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud |
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| Publication Number | Publication Date |
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| CN110100735A true CN110100735A (en) | 2019-08-09 |
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| CN201910520121.1A Pending CN110100735A (en) | 2019-06-17 | 2019-06-17 | A kind of method of tissue culture loud, high-pitched sound apple seedling adventitious root Regeneration in Vitro adventitious bud |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111793636A (en) * | 2020-07-29 | 2020-10-20 | 山东农业大学 | Apple gene MdBT2 for regulating and controlling adventitious root development and application thereof |
| CN115896160A (en) * | 2022-10-28 | 2023-04-04 | 西北农林科技大学 | Method for efficiently and quickly obtaining stable transgenic plants of apples by utilizing agrobacterium rhizogenes |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577898A (en) * | 2011-01-09 | 2012-07-18 | 焦世德 | Propagation method of apple seedlings and application of apple seedlings |
| CN104115747A (en) * | 2014-07-03 | 2014-10-29 | 山东农业大学 | Production of apple flavonoids by using tissue culture method |
| CN105993963A (en) * | 2016-07-26 | 2016-10-12 | 象山宏森源农产品开发有限公司 | Apple rootstock tissue culture and propagation technology |
| EP3153014A1 (en) * | 2014-06-06 | 2017-04-12 | Riken | Callus inducing agent and callus induction method |
| CN106613933A (en) * | 2015-10-28 | 2017-05-10 | 北京市农林科学院 | Method for inducing rooting of apple dwarf rootstock tissue culture seedling |
| CN106857245A (en) * | 2017-01-04 | 2017-06-20 | 西北农林科技大学 | A kind of method for improving apple transgenic line regeneration efficiency |
| CN107155889A (en) * | 2017-06-05 | 2017-09-15 | 河池市智汇科技咨询有限公司 | The method of dendrobium candidum cultured in vitro regeneration plant |
| CN107439372A (en) * | 2017-07-11 | 2017-12-08 | 北京市农林科学院 | A kind of quick breeding Dwarf Stocks For Apple Trees C.G 935 method |
-
2019
- 2019-06-17 CN CN201910520121.1A patent/CN110100735A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577898A (en) * | 2011-01-09 | 2012-07-18 | 焦世德 | Propagation method of apple seedlings and application of apple seedlings |
| EP3153014A1 (en) * | 2014-06-06 | 2017-04-12 | Riken | Callus inducing agent and callus induction method |
| CN104115747A (en) * | 2014-07-03 | 2014-10-29 | 山东农业大学 | Production of apple flavonoids by using tissue culture method |
| CN106613933A (en) * | 2015-10-28 | 2017-05-10 | 北京市农林科学院 | Method for inducing rooting of apple dwarf rootstock tissue culture seedling |
| CN105993963A (en) * | 2016-07-26 | 2016-10-12 | 象山宏森源农产品开发有限公司 | Apple rootstock tissue culture and propagation technology |
| CN106857245A (en) * | 2017-01-04 | 2017-06-20 | 西北农林科技大学 | A kind of method for improving apple transgenic line regeneration efficiency |
| CN107155889A (en) * | 2017-06-05 | 2017-09-15 | 河池市智汇科技咨询有限公司 | The method of dendrobium candidum cultured in vitro regeneration plant |
| CN107439372A (en) * | 2017-07-11 | 2017-12-08 | 北京市农林科学院 | A kind of quick breeding Dwarf Stocks For Apple Trees C.G 935 method |
Non-Patent Citations (3)
| Title |
|---|
| JIAO WU等: "High-efficiency regeneration of Agrobacterium rhizogenes-induced hairy root in apple rootstock Malus baccata (L.) Borkh", 《PLANT CELL TISS ORGAN CULT》 * |
| 卢美凤: "大叶藻ZomNHX2基因克隆分析与苹果组培及耐盐初步研究", 《中国优秀博硕士学位论文全文数据库(硕士)》 * |
| 郑汉臣主编: "《药用植物学》", 30 November 1999, 人民卫生出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111793636A (en) * | 2020-07-29 | 2020-10-20 | 山东农业大学 | Apple gene MdBT2 for regulating and controlling adventitious root development and application thereof |
| CN115896160A (en) * | 2022-10-28 | 2023-04-04 | 西北农林科技大学 | Method for efficiently and quickly obtaining stable transgenic plants of apples by utilizing agrobacterium rhizogenes |
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Application publication date: 20190809 |