CN110057926A - Detect the method with complete polar head compound in archaeal cell membrane lipid - Google Patents
Detect the method with complete polar head compound in archaeal cell membrane lipid Download PDFInfo
- Publication number
- CN110057926A CN110057926A CN201810342391.3A CN201810342391A CN110057926A CN 110057926 A CN110057926 A CN 110057926A CN 201810342391 A CN201810342391 A CN 201810342391A CN 110057926 A CN110057926 A CN 110057926A
- Authority
- CN
- China
- Prior art keywords
- polar head
- detection
- cell membrane
- compound
- membrane lipid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The present invention provides a kind of method with complete polar head compound in detection archaeal cell membrane lipid, comprising the following steps: chromatogram column temperature is set as 40~50 DEG C at MRM, flow velocity is 0.15~0.25mL/min；Control ion source dry gas stream 2.5~3.5L/min of speed, 280~320 DEG C of temperature；8.5~9.5L/min of sheath gas, 280~320 DEG C of temperature；400~450kPa of atomisation pressure；3.5~5.0kV of capillary voltage；650~750V of injection electric；150~250 instrument units of voltage of fragmentation reaction, 10~50 instrument units of collision energy；Core rouge part mass-to-charge ratio without any polar head is 1302.3~1292.3；Carry out the detection with complete polar head compound in archaeal cell membrane lipid.The present invention has the characteristics that detection sensitivity is high, detection limit is low.
Description
Technical field
The invention belongs to archaeal cell membrane lipid detection technique fields, and in particular to complete in a kind of detection archaeal cell membrane lipid
The method of whole polar head compound.
Background technique
Important branch of the archaeal as three domain theory of life, due to its distinctive 16SrRNA so that its with other two big points
Branch bacterium and eucaryote separate.Pass through the research formed to archaeal cell membrane, it has been found that archaeal cell membrane and bacterium are thin
There are significant differences for after birth: the cell membrane of archaeal is made of single layer glycerophosphatide molecular layer, and core element mainly contains
The glycerol dioxane glycerol being made of the isoprenoid saturated alkane carbochain that 4 ehter bonds and two sections 40 or more carbon molecules form
Tetraether rouge structure (GDGTs).Wherein, complete Polar Crystal Slab rouge (IPL) is connected with one or two not in glycerin ether rouge core chain
Stable polar head, predominantly containing sugar or phosphorous group.Complete Polar Crystal Slab rouge is degraded rapidly (White after cell death
Et al., 1979), it is converted into core lipoid substance (CL).Therefore, complete Polar Crystal Slab compound can be used to assessment certainly
The biomass (Pitcher et al., 2012) of living body archaeal in right environment.
Constantly developing for the detection method and means of the complete polarity lipoid substance of archaeal, currently used test
Means are to choose positive-liquid chromatogram-electron spray-multi-stage ms (NP-LC-ESI-MSn) method (Sturt et al., 2004；
Zink et al., 2003), specially first with the IPL compound of normal-phase chromatography separation opposed polarity head, recycle ion trap
While mass spectrum is to compound test, parsed by structure of the multiple stage crushing to compound (Schouten et al.,
2008；Sturt et al.,2004).And Zhu et al. (2013) is established using reversed-phase liquid chromatography and flight time mass spectrum
New detection method: reverse phase-liquid chromatogram-electron spray-mass spectrum (RP-LC-ESI-MS).This method can not only separate not
The IPL compound of same polarity head, moreover it is possible to separate the chemical combination for having different five-membered rings and double bond in IPL compound on core carbon skeleton
Object.This provides more information (Zhu et al., 2013) for the diversity of IPL compound.But studies have shown that NP-
LC-ESI-MSnIn method, even if the structure of core has differences, the compound with same polar head still can be common
It overflows.Therefore, this analysis method can lose effective information provided by nuclear structure in IPL compound, and ion trap matter
Although spectrum energy authenticating compound structure, resolution ratio is low, detects limit for height, can not wide popularization and application.Although RP-LC-ESI-MS
Can separate more IPL compounds, but its detection is established in the full scan mode of flight time mass spectrum, i.e., ion without
It crosses and is crushed all directly by Mass Spectrometer Method, this mode noise is big, IPL compound lower for content in environmental sample
Identification result is poor.
Summary of the invention
It is total with identical polar head compound present in complete Polar Crystal Slab compound detection process for current archaeal
Outflow, resolution ratio is low, detection limit for height, the problems such as sensitivity is low and noise is big, and the present invention provides a kind of detection archaeal cell membrane lipid
The method of the middle complete polar head compound of band.
For achieving the above object, technical scheme is as follows:
A method of with complete polar head compound in detection archaeal cell membrane lipid, at least include the following steps:
Under triple level four bars mass spectrum multiple reaction monitoring patterns, chromatogram column temperature is set as 40~50 DEG C, controls color
Spectrum column flow rate is 0.15~0.25mL/min；
Control ion source dry gas stream speed is 2.5~3.5L/min, and temperature is 280~320 DEG C；Sheath gas be 8.5~
9.5L/min, temperature are 280~320 DEG C；Atomisation pressure is 400~450kPa；3.5~5.0kV of capillary voltage；Injection electric
650~750V；
The voltage of fragmentation reaction is 150~250 instrument units, and collision energy is 10~50 instrument units；It is anti-to control fragmentation
The daughter ion mass-to-charge ratio that should be generated is as follows: the core rouge part mass-to-charge ratio without any polar head is 1302.3~1292.3；
Under the above conditions, the detection with complete polar head compound in archaeal cell membrane lipid is carried out, and records detection knot
Fruit.
The beneficial effects of the present invention are: compared with the existing technology, the present invention is examined in triple level four bars mass spectrum multiple reactions
Under survey mode, the screening to ion is realized, under the premise of combining testing conditions, on the one hand solves identical polar head chemical combination
The problem of object flows out altogether can isolate more complete Polar Crystal Slab rouge；On the other hand, moreover it is possible to reduce noise, reduce complete pole
Property film rouge detection limit, improve resolution ratio and sensitivity.In addition, this method has the characteristics that easy to operate, plant maintenance is convenient,
It is suitble in the detection for being widely used in archaeal cell membrane.
Detailed description of the invention
It to describe the technical solutions in the embodiments of the present invention more clearly, below will be to needed in the embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for ability
For the those of ordinary skill of domain, without creative efforts, it can also be obtained according to these attached drawings other attached
Figure.
Fig. 1 be detection archaeal cell membrane lipid provided by the invention in the method with complete polar head compound and select from
Sub- monitoring pattern is to monosaccharide GDGTs compound (1G-GDGTs), disaccharides GDGTs compound (2G-GDGTs), 2, the band phosphorus of sugar 1
Sour GDGTs compound (HPH-GDGTs) carries out detected comparison map；
Fig. 2 is that the sample in varying environment source uses Salbutamol Selected Ion Monitoring mode and method provided by the invention to list respectively
The TEX that sugared GDGTs compound calculates86Index (1G-TEX86) carry out repeated comparison；
Fig. 3 is that the sample in varying environment source uses Salbutamol Selected Ion Monitoring mode and method provided by the invention to two respectively
The TEX that sugared GDGTs compound calculates86(2G-TEX86) carry out repeated comparison；
Wherein, GDGT-0 is indicatedGDGT-1 is indicated
GDGT-2 is indicated
GDGT-3 is indicated
GDGT-4 is indicated
Gren is indicated
Gren ' expression
1G-GDGTs is indicated
2G-GDGTs is indicated
HPH-GDGTs is indicated
1G-GDGT-0 then indicates that the GDGTs in 1G-GDGTs is GDGT-0, other 1G-GDGT-1,1G-GDGT-2,1G-
GDGT-3、1G-GDGT-4、1G-Gren、1G-Gren、2G-GDGT-0、2G-GDGT-1、2G-GDGT-2、2G-GDGT-3、2G-
GDGT-4、2G-Gren、2G-Gren、HPH-GDGT-0、HPH-GDGT-1、HPH-GDGT-2、HPH-GDGT-3、HPH-GDGT-
4, HPH-Gren ', HPH-Gren etc. analogize；
PR-sed indicates Zhujiang River sediment sample；LZL-sed indicates Sediment from Liangzi Lake sample；XSBN-soil indicates west
Double versions receive pedotheque；N.A. indicate that index fails to calculate；In addition, SIM indicates Salbutamol Selected Ion Monitoring mode in Fig. 1；MRM table
Show monitoring pattern of the invention；Intensity indicates intensity；Retention time indicates retention time；N.D. indicating can not
Detection.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, not
For limiting the present invention.
Present example provides a kind of method with complete polar head compound in detection archaeal cell membrane lipid, includes at least
Following steps:
Under triple level four bars mass spectrum multiple reaction monitoring patterns, chromatogram column temperature is set as 40~50 DEG C, controls color
Spectrum column flow rate is 0.15~0.25mL/min；
Control ion source dry gas stream speed is 2.5~3.5L/min, and temperature is 280~320 DEG C；Sheath gas be 8.5~
9.5L/min, temperature are 280~320 DEG C；Atomisation pressure is 400~450kPa；3.5~5.0kV of capillary voltage；Injection electric
650~750V；
The voltage of fragmentation reaction is 150~250 instrument units, and collision energy is 10~50 instrument units；It is anti-to control fragmentation
The daughter ion mass-to-charge ratio that should be generated is as follows: the core rouge part mass-to-charge ratio without any polar head is 1302.3~1292.3；
Under the above conditions, the detection with complete polar head compound in archaeal cell membrane lipid is carried out, and records detection knot
Fruit.
Technical solution of the present invention is further explained in detail below.
In reversed-phase liquid chromatography condition of the invention, chromatographic column is the C of ACE318Packing material analytical column, packing material point
The partial size for analysing column is 3 μm, internal diameter 2.1mm, length 150nm.Mobile phase A is methanol, formic acid, ammonium hydroxide mixed solution, and is pressed
It is methanol: formic acid: ammonium hydroxide=100:0.04:0.10 according to volume ratio.
Mobile phase B is isopropanol, formic acid, ammonium hydroxide mixed solution, and is isopropanol: formic acid according to volume ratio: ammonium hydroxide=
100:0.04:0.10；45 DEG C of column temperature；Flow velocity 0.2mL/min.Eluent gradient: 100% mobile phase A (10min), 24% flowing
Phase B (5min), Mobile phase B to 24% change of gradient to 65% after (experience 55min), rinsed with 90% Mobile phase B, finally with
100% mobile phase A balances chromatographic column.Specific chromatography column parameter can be with reference to the reverse phase liquid that Zhu et al. (2013) is delivered
Chromatographic condition.
Ion source used in the present invention is the ESI ion source in Agilent Jet Stream technology.Preferably, ion source
Dry gas stream speed is 3.0L/min, and temperature is 300 DEG C；Sheath gas is 9.0L/min, and temperature is 300 DEG C；Atomisation pressure is
414kPa；Capillary voltage 5.0kV；Injection electric 700V.Under the conditions of the ion source, the corresponding highest of the mass signal of acquisition,
And most stable, noise is few.
Preferably, the voltage of fragmentation reaction is 210 instrument units, and collision energy is 35 instrument units；Control fragmentation reaction
The daughter ion mass-to-charge ratio of generation is as follows: the core rouge part mass-to-charge ratio without any polar head is 1302.3~1292.3.
Under the above conditions, the glycosyl fragmentation reaction of untested compound is stablized, and compound separation is obvious, and peak type is clearly easy to
Identification, and noise minimizes.
In embodiment, mass-to-charge ratio is according to the mass-to-charge ratio of ammonium ion form, i.e. m/z [M+NH4 +]。
For different compounds (IPL-GDGTs compound), mother ion mass-to-charge ratio and corresponding daughter ion mass-to-charge ratio according to
Concrete condition is replaced, meanwhile, corresponding collision energy can also be replaced, and common compound includes containing 1 glycosyl
Class GDGTs compound (or HO-GDGTs compound), 2 glycosyl class GDGTs compounds (or HO-GDGTs compound) and band 2
The HPH-GDGTs compound of a glycosyl and 1 phosphate group, specifically can be as shown in table 1.Wherein, it can be used for the HO- replaced
GDGTs compound includes
1G-OH-GDGTs:
2G-OH-GDGTs:
There is OH-GDGT-0 in concrete case:
OH-GDGT-1:
OH-GDGT-2:
Specifically, 1G-OH-GDGT-0 indicate 1G-OH-GDGTs in GDGTs be OH-GDGT-0,
1G-OH-GDGT-1 indicates that the GDGTs in 1G-OH-GDGTs is OH-GDGT-1,
1G-OH-GDGT-2 indicates that the GDGTs in 1G-OH-GDGTs is OH-GDGT-2, other are analogized.
Table 1 is directed to the collision energy of different IPL-GDGTs compounds
More effectively to illustrate technical solution of the present invention, technology of the invention is illustrated below by multiple specific embodiments
Scheme.
Embodiment 1
A method of with complete polar head compound in detection archaeal cell membrane lipid, this method specifically includes following step
It is rapid:
Under triple level four bars mass spectrum multiple reaction monitoring patterns (MRM), chromatogram column temperature is set as 45 DEG C, flow velocity is
0.2mL/min, ion source dry gas stream speed are 3.0L/min, and temperature is 300 DEG C；Sheath gas is 9.0L/min, temperature 300
℃；Atomisation pressure is 414kPa；Capillary voltage 5.0kV；Injection electric 700V；The voltage of fragmentation reaction is 210 instrument units,
Collision energy is 35 instrument units；Controlling the daughter ion mass-to-charge ratio that fragmentation reaction generates is 1302.3~1292.3, is then detected
Monosaccharide GDGTs compound (1G-GDGTs), testing result is as shown in Figure 1.
Comparative example 1
A method of with complete polar head compound in detection archaeal cell membrane lipid, this method specifically includes following step
It is rapid:
In the case where selecting ion monitoring mode (SIM), chromatogram column temperature is set as 45 DEG C, flow velocity 0.2mL/min, ion source
Dry gas stream speed is 3.0L/min, and temperature is 300 DEG C；Sheath gas is 9.0L/min, and temperature is 300 DEG C；Atomisation pressure is
414kPa；Capillary voltage 5.0kV；Injection electric 700V；The voltage of fragmentation reaction is 135 instrument units；Then monosaccharide is detected
GDGTs compound (1G-GDGTs), testing result is as shown in Figure 1.
As shown in Figure 1, under same concentrations, using under triple level four bars mass spectrum multiple reaction monitoring patterns (MRM) and phase
Under the detection parameters answered, relative to selection ion monitoring mode sensitivity is higher, peak type is more preferable, detection limit is lower, and detect
Compound it is more, signal-to-noise ratio (S/N) is higher.
Embodiment 2
A method of with complete polar head compound in detection archaeal cell membrane lipid, this method specifically includes following step
It is rapid:
Under triple level four bars mass spectrum multiple reaction monitoring patterns (MRM), chromatogram column temperature is set as 45 DEG C, flow velocity is
0.2mL/min, ion source dry gas stream speed are 3.0L/min, and temperature is 300 DEG C；Sheath gas is 9.0L/min, temperature 300
℃；Atomisation pressure is 414kPa；Capillary voltage 5.0kV；Injection electric 700V；The voltage of fragmentation reaction is 210 instrument units,
Collision energy is 35 instrument units；Controlling the daughter ion mass-to-charge ratio that fragmentation reaction generates is 1302.3~1292.3, is then detected
Disaccharides GDGTs compound (2G-GDGTs), testing result is as shown in Figure 1.
Comparative example 2
A method of with complete polar head compound in detection archaeal cell membrane lipid, this method specifically includes following step
It is rapid:
In the case where selecting ion monitoring mode (SIM), chromatogram column temperature is set as 45 DEG C, flow velocity 0.2mL/min, ion source
Dry gas stream speed is 3.0L/min, and temperature is 300 DEG C；Sheath gas is 9.0L/min, and temperature is 300 DEG C；Atomisation pressure is
414kPa；Capillary voltage 5.0kV；Injection electric 700V；The voltage of fragmentation reaction is 135 instrument units；Then disaccharides is detected
GDGTs compound (2G-GDGTs), testing result is as shown in Figure 1.
As shown in Figure 1, under same concentrations, using under triple level four bars mass spectrum multiple reaction monitoring patterns (MRM) and phase
Under the detection parameters answered, relative to selection ion monitoring mode sensitivity is higher, peak type is more preferable, detection limit is lower, and detect
Compound it is more, signal-to-noise ratio (S/N) is higher.
Embodiment 3
A method of with complete polar head compound in detection archaeal cell membrane lipid, this method specifically includes following step
It is rapid:
Under triple level four bars mass spectrum multiple reaction monitoring patterns (MRM), chromatogram column temperature is set as 45 DEG C, flow velocity is
0.2mL/min, ion source dry gas stream speed are 3.0L/min, and temperature is 300 DEG C；Sheath gas is 9.0L/min, temperature 300
℃；Atomisation pressure is 414kPa；Capillary voltage 5.0kV；Injection electric 700V；The voltage of fragmentation reaction is 210 instrument units,
Collision energy is 35 instrument units；Controlling the daughter ion mass-to-charge ratio that fragmentation reaction generates is 1544.3~1534.3, is then detected
2, the band phosphoric acid GDGTs compound of sugar 1 (HPH-GDGTs), testing result is as shown in Figure 1.
Comparative example 3
A method of with complete polar head compound in detection archaeal cell membrane lipid, this method specifically includes following step
It is rapid:
In the case where selecting ion monitoring mode (SIM), chromatogram column temperature is set as 45 DEG C, flow velocity 0.2mL/min, ion source
Dry gas stream speed is 3.0L/min, and temperature is 300 DEG C；Sheath gas is 9.0L/min, and temperature is 300 DEG C；Atomisation pressure is
414kPa；Capillary voltage 5.0kV；Injection electric 700V；The voltage of fragmentation reaction is 135 instrument units；Then 2, band are detected
Sugared 1 phosphoric acid GDGTs compound (HPH-GDGTs), testing result is as shown in Figure 1.
As shown in Figure 1, under same concentrations, using under triple level four bars mass spectrum multiple reaction monitoring patterns (MRM) and phase
Under the detection parameters answered, relative to selection ion monitoring mode sensitivity is higher, peak type is more preferable, detection limit is lower, and detect
Compound it is more, signal-to-noise ratio (S/N) is higher.
Embodiment 4
It is heavy to Zhujiang River sediment sample (PR-sed), Liangzi Lake respectively using the test method of embodiment 1 and comparative example 1
Product object sample (LZL-sed), Xishuangbanna pedotheque (XSBN-soil) are tested, each sample retest 5 times, and
Calculate common Organic Geochemistry Indexes TEX86(four ether indexes), test result method of the invention as shown in Figures 2 and 3 is more
Add and stablize, is repeated more preferable, standard deviation S D is 0.011~0.034, and the method for ion monitoring mode is selected to limit due to detection
Height, part of compounds is without response, that is, can not detect, and therefore, selects ion monitoring mode detection effect bad, and repeat
Property is poor.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc. within mind and principle should all include within protection scope of the present invention.
Claims (4)
1. a kind of method with complete polar head compound in detection archaeal cell membrane lipid, which is characterized in that include at least following
Step:
Under triple level four bars mass spectrum multiple reaction monitoring patterns, chromatogram column temperature is set as 40~50 DEG C, controls chromatographic column
Flow velocity is 0.15~0.25mL/min；
Control ion source dry gas stream speed is 2.5~3.5L/min, and temperature is 280~320 DEG C；Sheath gas is 8.5~9.5L/
Min, temperature are 280~320 DEG C；Atomisation pressure is 400~450kPa；3.5~5.0kV of capillary voltage；Injection electric 650~
750V；
The voltage of fragmentation reaction is 150~250 instrument units, and collision energy is 10~50 instrument units；Fragmentation reaction is controlled to produce
Raw daughter ion mass-to-charge ratio is as follows: the core rouge part mass-to-charge ratio without any polar head is 1302.3~1292.3；
Under the above conditions, the detection with complete polar head compound in archaeal cell membrane lipid is carried out, and records testing result.
2. the method with complete polar head compound in detection archaeal cell membrane lipid as described in claim 1, which is characterized in that
Mobile phase A is the mixed solution of methanol, formic acid, ammonium hydroxide in the chromatographic column；The methanol, formic acid, ammonium hydroxide mixed solution in,
It is methanol: formic acid: ammonium hydroxide=100:0.04:0.10 according to volume ratio.
3. the method with complete polar head compound in detection archaeal cell membrane lipid as described in claim 1, which is characterized in that
Mobile phase B is the mixed solution of isopropanol, formic acid, ammonium hydroxide in the chromatographic column；The isopropanol, formic acid, the mixing of ammonium hydroxide are molten
It is isopropanol: formic acid: ammonium hydroxide=100:0.04:0.10 according to volume ratio in liquid.
4. the method with complete polar head compound in detection archaeal cell membrane lipid as described in claim 1, which is characterized in that
The chromatographic column is the C of ACE318Packing material analytical column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810342391.3A CN110057926A (en) | 2018-04-17 | 2018-04-17 | Detect the method with complete polar head compound in archaeal cell membrane lipid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810342391.3A CN110057926A (en) | 2018-04-17 | 2018-04-17 | Detect the method with complete polar head compound in archaeal cell membrane lipid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110057926A true CN110057926A (en) | 2019-07-26 |
Family
ID=67314990
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810342391.3A Pending CN110057926A (en) | 2018-04-17 | 2018-04-17 | Detect the method with complete polar head compound in archaeal cell membrane lipid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110057926A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103293259A (en) * | 2012-02-24 | 2013-09-11 | 华东理工大学 | Method for analyzing composition of fatty acid in Corynebacterium glutamicum cell membrane |
| CN103884782A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Large-scale blood plasma sphingolipid profile analysis method based on liquid chromatography-mass spectrometry combination |
| CN105136944A (en) * | 2015-09-08 | 2015-12-09 | 重庆大学 | Ultrasonic extraction method of complete ladderane lipid in anaerobic ammonium oxidation bacteria |
| CN107677748A (en) * | 2017-10-09 | 2018-02-09 | 贵州省疾病预防控制中心 | The rapid screening detection method of perfluorochemical in a kind of breast milk |
-
2018
- 2018-04-17 CN CN201810342391.3A patent/CN110057926A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103293259A (en) * | 2012-02-24 | 2013-09-11 | 华东理工大学 | Method for analyzing composition of fatty acid in Corynebacterium glutamicum cell membrane |
| CN103884782A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Large-scale blood plasma sphingolipid profile analysis method based on liquid chromatography-mass spectrometry combination |
| CN105136944A (en) * | 2015-09-08 | 2015-12-09 | 重庆大学 | Ultrasonic extraction method of complete ladderane lipid in anaerobic ammonium oxidation bacteria |
| CN107677748A (en) * | 2017-10-09 | 2018-02-09 | 贵州省疾病预防控制中心 | The rapid screening detection method of perfluorochemical in a kind of breast milk |
Non-Patent Citations (4)
| Title |
|---|
| CHUN ZHU 等: "Comprehensive glycerol ether lipid fingerprints through a novel reversed phase liquid chromatography–mass spectrometry protocol", 《ORGANIC GEOCHEMISTRY》 * |
| SAITO, RYOSUKE 等: "Tentative identification of diagenetic products of cyclic biphytanes in sedimentary rocks from the uppermost Permian and Lower Triassi", 《ORGANIC GEOCHEMISTRY》 * |
| YUFEI CHEN 等: "Tracking the signals of living archaea: A multiple reaction monitoring (MRM) method for detection of trace amounts of intact polar lipids from the natural environment", 《ORGANIC GEOCHEMISTRY》 * |
| 姚鹏 等: "海洋沉积物中现存微生物化学标志物完整极性膜脂研究进展", 《地球科学进展》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dong et al. | Determination of organochlorine pesticides and their metabolites in radish after headspace solid-phase microextraction using calix [4] arene fiber | |
| Liu et al. | Position-specific carbon and hydrogen isotopic compositions of propane from natural gases with quantitative NMR | |
| CN107247093A (en) | The detection method of free metanephrine class material in urine | |
| Brenna | High-precision gas isotope ratio mass spectrometry: recent advances in instrumentation and biomedical applications | |
| Zhou et al. | Solid-phase microextraction coupled with capillary electrophoresis for the determination of propranolol enantiomers in urine using a sol–gel derived calix [4] arene fiber | |
| Yang et al. | Twenty‐one years of microemulsion electrokinetic chromatography (1991–2012): A powerful analytical tool | |
| Liu et al. | Hydrogen isotopic compositions of long-chain leaf wax n-alkanes in Lake Qinghai sediments record palaeohydrological variations during the past 12 ka | |
| Wang et al. | Graphene oxide as a micro‐solid‐phase extraction sorbent for the enrichment of parabens from water and vinegar samples | |
| CN103575845B (en) | Comprehensive two-dimensional gas chromatography quantitative analysis method of aromatic sterane compound in petroleum | |
| CN105987946B (en) | The tracing method in one oil migration direction | |
| CN106198474B (en) | A kind of mercury ion test paper and its application method | |
| Jiang et al. | Tracing methane dissolved in transformer oil by tunable diode laser absorption spectrum | |
| Barra et al. | Deconvolution of post-and pre-salt petroleum sources in southeastern offshore Brazilian basins | |
| CN108693280A (en) | The method for quantitative determining the Sino-German paddy insulin content of biological sample by UPLC-MS/MS | |
| Liu et al. | Inter-molecular variations of fatty acid δD in algae and submerged plants from the north-eastern Tibetan Plateau | |
| Liu et al. | Macrocyclic polyamine‐functionalized silica as a solid‐phase extraction material coupled with ionic liquid dispersive liquid–liquid extraction for the enrichment of polycyclic aromatic hydrocarbons | |
| Xu et al. | Compound-specific radiocarbon analysis of low molecular weight dicarboxylic acids in ambient aerosols using preparative gas chromatography: method development | |
| CN110057926A (en) | Detect the method with complete polar head compound in archaeal cell membrane lipid | |
| Radovic et al. | Rapid screening of glycerol ether lipid biomarkers in recent marine sediment using atmospheric pressure photoionization in positive mode Fourier transform ion cyclotron resonance mass spectrometry | |
| Hu et al. | Determination of neotame in non‐alcoholic beverage by capillary zone electrophoresis | |
| Scholten et al. | Identification of ergot alkaloids with a photochemical reaction detector in liquid chromatography | |
| CN103808822A (en) | LC-QTOF (Liquid Chromatography-Quadrupole Time Of Flight) analysis method for distinguishing resveratrol of different resources | |
| CN104502486B (en) | A kind of apply the method for methyl vanillin and ethyl vanillin in Headspace-solid phase microextraction technical measurement milk powder | |
| Zhang et al. | Long-term drying trends since the mid-Holocene in the Qaidam Basin | |
| Yan et al. | Habitat influence on the molecular, carbon and hydrogen isotope compositions of leaf wax n-alkanes in a subalpine basin, central China |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190726 |
|
| RJ01 | Rejection of invention patent application after publication |