CN110057609B - Reference sample for testing preservation effect and preparation method and application thereof - Google Patents
Reference sample for testing preservation effect and preparation method and application thereof Download PDFInfo
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Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01M—TESTING STATIC OR DYNAMIC BALANCE OF MACHINES OR STRUCTURES; TESTING OF STRUCTURES OR APPARATUS, NOT OTHERWISE PROVIDED FOR
- G01M99/00—Subject matter not provided for in other groups of this subclass
- G01M99/008—Subject matter not provided for in other groups of this subclass by doing functionality tests
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Sampling And Sample Adjustment (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
技术领域technical field
本发明涉及保鲜性能检测技术领域,尤其是涉及一种保鲜效果测试用参照样及其制备方法和应用。The invention relates to the technical field of fresh-keeping performance detection, in particular to a reference sample for fresh-keeping effect testing and a preparation method and application thereof.
背景技术Background technique
冰箱是我们日常生活中食品保藏保鲜的首选器具。各个冰箱制造商为了满足消费者对保鲜的需求,不断对冰箱的储藏空间的保鲜能力进行着升级换代。目前对冰箱保鲜效果的评价,主要采用新鲜蔬菜如菠菜在冰箱冷藏区储存一定的周期后(如3天或4天)检测其中的水分、维生素c、维生素E、叶绿素、维生素B族、叶酸等指标的变化情况,通过分析各个冰箱对各个指标的保持情况,来评价保鲜效果。The refrigerator is the first choice for food preservation in our daily life. In order to meet consumers' demand for fresh-keeping, various refrigerator manufacturers are constantly upgrading the fresh-keeping ability of the storage space of refrigerators. At present, the evaluation of the preservation effect of refrigerators mainly uses fresh vegetables such as spinach to be stored in the refrigerator for a certain period of time (such as 3 or 4 days) to detect the moisture, vitamin C, vitamin E, chlorophyll, vitamin B family, folic acid, etc. The changes of the indicators are evaluated by analyzing the maintenance of each indicator by each refrigerator to evaluate the preservation effect.
目前采用新鲜蔬菜作为储藏对象来评价保鲜效果的方法存在以下缺点:一是受蔬菜的采收季节的影响较大,非时令季节该蔬菜的供应难以有效保障;二是新鲜蔬菜的均匀性、产品性状的稳定性难以保障。这些缺点易造成实验误差较大,且重复性较差,在不同实验室间难以开展良好的比对工作。At present, the method of using fresh vegetables as the storage object to evaluate the effect of preservation has the following shortcomings: First, it is greatly affected by the harvesting season of vegetables, and the supply of the vegetables in non-seasonal seasons is difficult to effectively guarantee; second, the uniformity of fresh vegetables, product quality The stability of traits is difficult to guarantee. These shortcomings easily lead to large experimental errors and poor repeatability, making it difficult to carry out good comparisons between different laboratories.
鉴于此,特提出本发明。In view of this, the present invention is proposed.
发明内容SUMMARY OF THE INVENTION
本发明的一个目的是提供一种保鲜效果测试用参照样,为解决现有技术中存在的采用新鲜蔬菜作为储藏对象评价保鲜效果的方法,一是受蔬菜的采收季节的影响较大,非时令季节该蔬菜的供应难以有效保障,二是新鲜蔬菜的均匀性、产品性状的稳定性难以保障,造成实验误差较大,且重复性较差,在不同实验室间难以开展良好的比对工作。该保鲜效果测试用参照样包括吸水材料,所述吸水材料内吸收有叶绿素、保湿剂和抗氧化剂的混合液。该参照样间的一致性较好,可广泛应用于冰箱的保鲜效果评价,实验误差较小,且重复性较好,在不同实验室间可开展良好的比对工作。One object of the present invention is to provide a reference sample for fresh-keeping effect test. In order to solve the method for evaluating fresh-keeping effect by using fresh vegetables as storage objects in the prior art, one is that it is greatly affected by the harvesting season of vegetables, and the It is difficult to effectively guarantee the supply of the vegetables in seasonal seasons. Second, the uniformity of fresh vegetables and the stability of product characteristics are difficult to guarantee, resulting in large experimental errors and poor repeatability, making it difficult to carry out good comparisons between different laboratories. . The reference sample for the fresh-keeping effect test includes a water-absorbing material, and the water-absorbing material absorbs a mixed solution of chlorophyll, a moisturizing agent and an antioxidant. The consistency between the reference samples is good, and it can be widely used in the evaluation of the preservation effect of refrigerators. The experimental error is small, and the repeatability is good. It can carry out good comparison work between different laboratories.
本发明的另一个目的是提供如上所述保鲜效果测试用参照样的制备方法,将吸水材料、叶绿素、保湿剂和抗氧化剂在液体环境下接触,工艺流程简单,制作效率较高,参照样样品间的一致性好,可广泛应用于冰箱、冰柜的保鲜效果评价,冰箱、冰柜保鲜评价测试的实验室间能力比对等,具有广泛的应用前景。Another object of the present invention is to provide the above-mentioned preparation method of the reference sample for fresh-keeping effect test, contact the water-absorbing material, chlorophyll, humectant and antioxidant in a liquid environment, the process is simple, the production efficiency is high, and the reference sample sample The consistency between them is good, and it can be widely used in the evaluation of the preservation effect of refrigerators and freezers, and the inter-laboratory ability comparison of refrigerator and freezer preservation evaluation tests, etc., and has broad application prospects.
本发明的另一个目的是提供如上所述的保鲜效果测试用参照样在检测冰箱和/或冰柜保鲜效果中的应用。Another object of the present invention is to provide the application of the above-mentioned reference sample for fresh-keeping effect test in detecting the fresh-keeping effect of refrigerators and/or freezers.
一种保鲜效果测试用参照样,包括吸水材料,所述吸水材料内吸收有叶绿素、保湿剂和抗氧化剂的混合液。A reference sample for fresh-keeping effect test includes a water-absorbing material, and the water-absorbing material absorbs a mixed solution of chlorophyll, a moisturizing agent and an antioxidant.
优选地,所述吸水材料装在透气隔水袋中;Preferably, the water-absorbing material is packed in a breathable water-proof bag;
优选地,所述透气隔水袋的规格为5-10cm×8-15cm,更优选为6-8cm×10-12cm。Preferably, the size of the air-permeable water barrier is 5-10cm×8-15cm, more preferably 6-8cm×10-12cm.
优选地,所述吸水材料为多层,每一层或每几层由防水材料隔开;Preferably, the water-absorbing material is multi-layered, and each layer or several layers are separated by a waterproof material;
优选地,所述吸水材料由5-10块叠放在一起;Preferably, the water-absorbing material is stacked together by 5-10 pieces;
优选地,每层所述吸水材料的尺寸相同或者尺寸不同;Preferably, the size of each layer of the water-absorbing material is the same or different;
优选地,所述吸水材料的规格为3-8cm×6-13cm,更优选为4-6cm×8-10cm。Preferably, the size of the water-absorbing material is 3-8cm×6-13cm, more preferably 4-6cm×8-10cm.
优选地,所述吸水材料选自无纺布、棉布或涤纶布中的至少一种;Preferably, the water-absorbing material is selected from at least one of non-woven fabrics, cotton fabrics or polyester fabrics;
优选地,所述防水材料选自PP膜、PE膜或PET膜中至少一种。Preferably, the waterproof material is selected from at least one of PP film, PE film or PET film.
优选地,所述抗氧化剂选自L-抗坏血酸、丁基羟基茴香醚、二丁基羟基甲苯和没食子酸丙酯中的至少一种。Preferably, the antioxidant is selected from at least one of L-ascorbic acid, butylated hydroxyanisole, dibutylated hydroxytoluene and propyl gallate.
优选地,所述保湿剂选自丙二醇、丙三醇和山梨醇中的至少一种。Preferably, the humectant is selected from at least one of propylene glycol, glycerol and sorbitol.
优选地,所述抗氧化剂和所述叶绿素的质量比为9-12:1,更优选为10-11:1。Preferably, the mass ratio of the antioxidant and the chlorophyll is 9-12:1, more preferably 10-11:1.
优选地,所述混合液的溶剂为水;Preferably, the solvent of the mixed solution is water;
优选地,所述水为脱氧水,且溶解有非氧化保护气体;Preferably, the water is deoxygenated water, and a non-oxidizing protective gas is dissolved;
优选地,所述非氧化保护气体为氮气。Preferably, the non-oxidizing protective gas is nitrogen.
如上所述的保鲜效果测试用参照样的制备方法,包括以下步骤:The preparation method of the reference sample for the fresh-keeping effect test as above, comprises the following steps:
将所述吸水材料、叶绿素、保湿剂和抗氧化剂在液体环境下接触;contacting the water-absorbing material, chlorophyll, humectant and antioxidant in a liquid environment;
优选地,所述接触的时间为4-8min,更优选为5-6min。Preferably, the contact time is 4-8 min, more preferably 5-6 min.
如上所述的保鲜效果测试用参照样在检测冰箱和/或冰柜保鲜效果中的应用。The application of the above-mentioned reference sample for fresh-keeping effect test in detecting the fresh-keeping effect of refrigerator and/or freezer.
与已有技术相比,本发明具有如下有益效果:Compared with the prior art, the present invention has the following beneficial effects:
(1)本发明中的保鲜效果测试用参照样样品间的一致性较好,可广泛应用于冰箱的保鲜效果评价,实验误差较小,且重复性较好,在不同实验室间可开展良好的比对工作。(1) The consistency between the reference samples for the fresh-keeping effect test in the present invention is good, and it can be widely used in the evaluation of the fresh-keeping effect of refrigerators. The experimental error is small, and the repeatability is good. comparison work.
(2)本发明中的保鲜效果测试用参照样的制备方法,将吸水材料、叶绿素、保湿剂和抗氧化剂在液体环境下接触,用隔水透气性较好的膜作为外包装,制作成参照样品。该制备工艺流程简单,制作效率较高,参照样样品间的一致性好,可广泛应用于冰箱、冰柜的保鲜效果评价,具有广泛的应用前景。(2) The preparation method of the reference sample for the fresh-keeping effect test in the present invention, the water-absorbing material, chlorophyll, humectant and antioxidant are contacted in a liquid environment, and a film with better water-permeability and air permeability is used as an outer package to make a reference sample. The preparation process is simple, the production efficiency is high, and the consistency between the reference samples is good, can be widely used in the evaluation of the preservation effect of refrigerators and freezers, and has wide application prospects.
附图说明Description of drawings
为了更清楚地说明本发明具体实施方式或现有技术中的技术方案,下面将对具体实施方式或现有技术描述中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图是本发明的一些实施方式,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他的附图。In order to illustrate the specific embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the specific embodiments or the prior art. Obviously, the accompanying drawings in the following description The drawings are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative efforts.
图1为本发明中的保鲜效果测试用参照样的制备工艺流程图。Fig. 1 is the preparation process flow chart of the reference sample for the preservation effect test in the present invention.
具体实施方式Detailed ways
下面将结合实施例对本发明的实施方案进行详细描述,但是本领域技术人员将会理解,下列实施例仅用于说明本发明,而不应视为限制本发明的范围。实施例中未注明具体条件者,按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者,均为可以通过市售购买获得的常规产品。The embodiments of the present invention will be described in detail below with reference to the examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. If the specific conditions are not indicated in the examples, it is carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used without the manufacturer's indication are conventional products that can be purchased from the market.
一种保鲜效果测试用参照样,包括吸水材料,所述吸水材料内吸收有叶绿素、保湿剂和抗氧化剂的混合液。A reference sample for fresh-keeping effect test includes a water-absorbing material, and the water-absorbing material absorbs a mixed solution of chlorophyll, a moisturizing agent and an antioxidant.
本发明中的保鲜效果测试用参照样样品间的一致性较好,可广泛应用于冰箱、冰柜的保鲜效果评价。The reference sample for the fresh-keeping effect test in the present invention has good consistency among the samples, and can be widely used in the evaluation of the fresh-keeping effect of refrigerators and freezers.
优选地,所述吸水材料装在透气隔水袋中;Preferably, the water-absorbing material is packed in a breathable water-proof bag;
优选地,所述透气隔水袋的规格为5-10cm×8-15cm,更优选为6-8cm×10-12cm。Preferably, the size of the air-permeable water barrier is 5-10cm×8-15cm, more preferably 6-8cm×10-12cm.
透气隔水袋的规格典型但非限制性的例如为5cm×8cm、6cm×9cm、7m×10cm、8cm×11cm、9cm×12cm或10cm×15cm。Typical but non-limiting dimensions of the breathable water barrier are, for example, 5 cm x 8 cm, 6 cm x 9 cm, 7 m x 10 cm, 8 cm x 11 cm, 9 cm x 12 cm or 10 cm x 15 cm.
优选地,所述吸水材料为多层,每一层或每几层由防水材料隔开;Preferably, the water-absorbing material is multi-layered, and each layer or several layers are separated by a waterproof material;
优选地,所述吸水材料由5-10块叠放在一起;Preferably, the water-absorbing material is stacked together by 5-10 pieces;
优选地,每层所述吸水材料的尺寸相同或者尺寸不同;Preferably, the size of each layer of the water-absorbing material is the same or different;
将多层吸水材料叠放在一起,每层吸水材料用防水材料隔开,可保证参照样样品间的一致性,使其用于评价冰箱或者冰柜保鲜效果时更具有准确性,实验误差较小,且重复性较好。Stacking multiple layers of water-absorbing materials together, each layer of water-absorbing material is separated by a waterproof material, can ensure the consistency between reference samples and make it more accurate when used to evaluate the preservation effect of refrigerators or freezers, and the experimental error is small , and the repeatability is good.
优选地,所述吸水材料的规格为3-8cm×6-13cm,更优选为4-6cm×8-10cm。Preferably, the size of the water-absorbing material is 3-8cm×6-13cm, more preferably 4-6cm×8-10cm.
吸水材料的规格典型但非限制性的例如为3cm×6cm、4cm×7cm、5cm×8cm、6cm×9cm、7cm×10cm或8cm×13cm。Typical but non-limiting dimensions of the water-absorbing material are, for example, 3 cm x 6 cm, 4 cm x 7 cm, 5 cm x 8 cm, 6 cm x 9 cm, 7 cm x 10 cm or 8 cm x 13 cm.
优选地,所述吸水材料选自无纺布、棉布或涤纶布中的至少一种;Preferably, the water-absorbing material is selected from at least one of non-woven fabrics, cotton fabrics or polyester fabrics;
优选地,所述吸水材料选自无纺布。无纺布又称不织布,是由定向的或随机的纤维而构成。因具有布的外观和某些性能而称其为布。多采用聚丙烯(pp材质)粒料为原料,经高温熔融、喷丝、铺纲、热压卷取连续一步法生产而成。具有柔软、透气和平面结构的新型纤维制品,优点是不产生纤维屑,强韧、耐用、丝般柔软,也是增强材料的一种,而且还有棉质的感觉,和棉织品相比,无纺布的袋子容易成形,而且造价便宜。本发明使用无纺布制作参照样更有利于成型,保证参照样样品间的一致性良好。Preferably, the water-absorbing material is selected from non-woven fabrics. Non-woven fabrics, also known as non-woven fabrics, are composed of oriented or random fibers. It is called cloth because of its appearance and certain properties. Polypropylene (pp material) pellets are mostly used as raw materials, and are produced by a continuous one-step method of high-temperature melting, spinning, laying, and hot-pressing and coiling. A new type of fiber product with a soft, breathable and flat structure, the advantage is that it does not produce lint, it is strong, durable, and silky soft, and it is also a kind of reinforcing material, and it also has a cotton feel. Compared with cotton fabrics, non-woven Cloth bags are easy to form and inexpensive to manufacture. In the present invention, the use of non-woven fabrics to make reference samples is more conducive to molding, and ensures good consistency between reference samples and samples.
优选地,所述防水材料选自PP膜、PE膜或PET膜中至少一种。Preferably, the waterproof material is selected from at least one of PP film, PE film or PET film.
优选地,所述抗氧化剂选自L-抗坏血酸、丁基羟基茴香醚、二丁基羟基甲苯和没食子酸丙酯中的至少一种。Preferably, the antioxidant is selected from at least one of L-ascorbic acid, butylated hydroxyanisole, dibutylated hydroxytoluene and propyl gallate.
抗氧化剂是指能防止或延缓食品氧化,提高食品的稳定性和延长贮存期的食品添加剂。抗氧化剂的正确使用不仅可以延长食品的贮存期、货架期,给生产者、消费者带来良好的经济效益,而且给消费者带来更好的食品安全。本发明优选采用L-抗坏血酸(维生素C),模拟菠菜的成分组成。Antioxidants refer to food additives that can prevent or delay the oxidation of food, improve the stability of food and prolong the storage period. The correct use of antioxidants can not only prolong the storage period and shelf life of food, bring good economic benefits to producers and consumers, but also bring better food safety to consumers. The present invention preferably adopts L-ascorbic acid (vitamin C) to simulate the composition of spinach.
优选地,所述保湿剂选自丙二醇、丙三醇和山梨醇中的至少一种。Preferably, the humectant is selected from at least one of propylene glycol, glycerol and sorbitol.
保湿剂是一类亲水性的润肤物质,在较低湿度范围内具有结合水的能力,给皮肤补充水分,它们可以通过控制产品与周围空气之间水分的交换使皮肤维持在高于正常水含量的平衡状态,起到减轻皮肤干燥的作用。本发明选用丙二醇、丙三醇和山梨醇中的至少一种作为保湿剂,可起到良好的保湿效果,有利于保鲜效果测试用参照样的样品间的一致性,使保鲜测试效果更加准确。Moisturizers are a class of hydrophilic emollients that have the ability to bind water in the lower humidity range, replenishing the skin with moisture, and they can maintain the skin above normal by controlling the exchange of moisture between the product and the surrounding air. Balanced state of water content, play a role in reducing dry skin. The present invention selects at least one of propylene glycol, glycerol and sorbitol as the moisturizing agent, which can play a good moisturizing effect, is beneficial to the consistency between the samples of the reference sample for the fresh-keeping effect test, and makes the fresh-keeping test effect more accurate.
优选地,所述抗氧化剂和所述叶绿素的质量比为9-12:1,更优选为10-11:1。Preferably, the mass ratio of the antioxidant and the chlorophyll is 9-12:1, more preferably 10-11:1.
抗氧化剂和叶绿素的质量比典型但非限制性的例如为9:1、10:1、11:1或12:1。Typical but non-limiting mass ratios of antioxidants and chlorophyll are, for example, 9:1, 10:1, 11:1 or 12:1.
优选地,所述混合液的溶剂为水;Preferably, the solvent of the mixed solution is water;
优选地,所述水为脱氧水,且溶解有非氧化保护气体;Preferably, the water is deoxygenated water, and a non-oxidizing protective gas is dissolved;
优选地,所述非氧化保护气体为氮气。Preferably, the non-oxidizing protective gas is nitrogen.
本发明根据制备的量量取超纯水,利用真空泵或其他脱气装置脱去超纯水中的氧气,脱氧时间为30min以上,优选为30-100min。In the present invention, the ultrapure water is measured according to the prepared amount, and the oxygen in the ultrapure water is removed by a vacuum pump or other degassing device, and the deoxygenation time is more than 30min, preferably 30-100min.
充氮气的过程为:把氮气瓶连接管路后,管口接近盛放纯水的容器底部,打开气阀,流量控制在200ml/min以内,备用。The process of filling nitrogen is as follows: after connecting the nitrogen bottle to the pipeline, the nozzle is close to the bottom of the container containing pure water, open the gas valve, and control the flow within 200ml/min for standby.
如上所述的保鲜效果测试用参照样的制备方法,包括以下步骤:The preparation method of the reference sample for the fresh-keeping effect test as above, comprises the following steps:
将所述吸水材料、叶绿素、保湿剂和抗氧化剂在液体环境下接触;该制作工艺流程简单,制作效率较高,参照样样品间的一致性好,可广泛应用于冰箱、冰柜的保鲜效果评价,冰箱、冰柜的保鲜评价测试的实验室间能力比对等,具有广泛的应用前景。The water-absorbing material, chlorophyll, humectant and antioxidant are contacted in a liquid environment; the production process is simple, the production efficiency is high, the consistency between reference samples and samples is good, and it can be widely used in the evaluation of the preservation effect of refrigerators and freezers , The inter-laboratory ability comparison of the fresh-keeping evaluation test of refrigerators and freezers, etc., has a wide range of application prospects.
优选地,所述接触的时间为4-8min,更优选为5-6min。Preferably, the contact time is 4-8 min, more preferably 5-6 min.
接触的时间典型但非限制性的例如为4min、5min、6min、7min或8min。Typical but non-limiting times of contact are, for example, 4 min, 5 min, 6 min, 7 min or 8 min.
在一种优选地实施方案中,所述保鲜效果测试用参照样的制备方法,包括以下步骤:In a preferred embodiment, the preparation method of the reference sample for the fresh-keeping effect test comprises the following steps:
(a)制作包装小袋:用透气隔水膜制作包装小袋,规格为5-10cm×8-15cm,缝制三边,留一边作为开口;(a) Making packaging pouches: making packaging pouches with a breathable water-permeable film, with a size of 5-10cm×8-15cm, sewing three sides, and leaving one side as an opening;
(b)制作吸水材料小块:把大尺寸的吸水材料叠放为5-10层,用裁剪工具把5-10层的吸水材料裁剪为小块,规格为3-8cm×6-10cm,每一份小块吸水材料用防水材料隔开,然后叠放在一起;(b) Making small pieces of water-absorbing material: stack the large-sized water-absorbing materials into 5-10 layers, and use a cutting tool to cut the 5-10 layers of water-absorbing material into small pieces, the size is 3-8cm×6-10cm, each A small portion of absorbent material separated by a waterproof material and stacked together;
(c)超纯水脱氧:根据制备的量量取超纯水,利用真空泵或其他脱气装置脱去超纯水中的氧气,脱氧时间为30-100min;(c) Deoxygenation of ultrapure water: measure ultrapure water according to the prepared amount, and use a vacuum pump or other degassing device to remove oxygen in the ultrapure water, and the deoxygenation time is 30-100min;
(d)充氮气:把氮气瓶连接管路后,管口接近盛放纯水的容器底部,打开气阀,流量控制在200ml/min以内,备用;(d) Nitrogen filling: after connecting the nitrogen bottle to the pipeline, the nozzle is close to the bottom of the container containing pure water, open the gas valve, and control the flow within 200ml/min for standby;
(e)称量试剂:用千分之一的天平称取抗氧化剂标准品;称取叶绿素标准品,抗氧化剂和叶绿素的质量比为9-12:1,用筒量取丙二醇;(e) Weighing reagents: Weigh the antioxidant standard product with a one-thousandth of the balance; Weigh the chlorophyll standard product, the mass ratio of antioxidant and chlorophyll is 9-12:1, and use a cylinder to measure propylene glycol;
(f)配置溶液:把称量好的叶绿素用量取的丙二醇溶解,然后倒入1000ml的容量瓶中,用适量(每次15ml左右)充过氮气的纯水洗涤量筒3次,洗涤液到入1000ml的容量瓶;添加称量好的抗氧化剂到1000ml的容量瓶中并定容至1000ml,盖盖,摇匀至抗氧化剂完全溶解;(f) Prepare the solution: dissolve the propylene glycol used for the weighed amount of chlorophyll, then pour it into a 1000ml volumetric flask, wash the graduated cylinder 3 times with an appropriate amount (about 15ml each time) of pure water filled with nitrogen, and the washing solution reaches the inlet 1000ml volumetric flask; add the weighed antioxidant to a 1000ml volumetric flask and dilute to 1000ml, cover and shake until the antioxidant is completely dissolved;
(g)浸泡:将用防水材料隔开且叠放在一起的吸水材料装入适宜的塑料自封袋中,倒入步骤(f)中的溶液(在氮气环境下操作)进行浸泡4-8min。(g) Soaking: The water-absorbing materials separated by waterproof materials and stacked together are put into suitable plastic ziplock bags, poured into the solution in step (f) (operated in nitrogen environment) and soaked for 4-8 minutes.
(h)装袋:把浸泡好的无纺布在一定压力下挤压去掉多余的液体,至不滴液为止,然后装入步骤(a)中的袋子,并封口,得到保鲜效果测试用参照样。(h) bagging: squeeze the soaked non-woven fabric under a certain pressure to remove excess liquid until no liquid drips, then put it into the bag in step (a), and seal it to obtain a reference for the preservation effect test Sample.
如上所述的保鲜效果测试用参照样在检测冰箱和/或冰柜保鲜效果中的应用。保鲜鲜果测试实验误差较小,且重复性较好,在不同实验室间可开展良好的比对工作。The application of the above-mentioned reference sample for fresh-keeping effect test in detecting the fresh-keeping effect of refrigerator and/or freezer. The experimental error of fresh-keeping fruit test is small, and the repeatability is good, and good comparison work can be carried out between different laboratories.
下面结合具体的实施例和附图,对本发明做进一步说明。The present invention will be further described below with reference to specific embodiments and accompanying drawings.
本发明实施例1-4中的保鲜效果测试用参照样的制备工艺流程图如图1所示。Figure 1 shows the flow chart of the preparation process of the reference sample for the preservation effect test in Examples 1-4 of the present invention.
实施例1Example 1
一种保鲜效果测试用参照样,包括吸水材料,并且吸水材料内吸收有叶绿素、保湿剂和抗氧化剂的混合液,每块吸水材料由防水材料隔开,装在透气隔水袋中;A reference sample for fresh-keeping effect test, comprising a water-absorbing material, and a mixed solution of chlorophyll, a moisturizing agent and an antioxidant is absorbed in the water-absorbing material, and each water-absorbing material is separated by a waterproof material and packed in a breathable water-proof bag;
其中,抗氧化剂为L-抗坏血酸,保湿剂为丙二醇,吸水材料为无纺布,无纺布的层数为5层,尺寸为6cm×9cm,防水材料为PE膜,透气隔水袋的规格为8cm×11cm;Among them, the antioxidant is L-ascorbic acid, the moisturizing agent is propylene glycol, the water-absorbing material is non-woven fabric, the number of layers of the non-woven fabric is 5, the size is 6cm×9cm, the waterproof material is PE film, and the specifications of the breathable water-proof bag are 8cm×11cm;
所述保鲜效果测试用参照样的制备方法,包括以下步骤:The preparation method of the reference sample for the fresh-keeping effect test comprises the following steps:
(a)用透气隔水膜制作包装小袋8cm×11cm,缝制三边,留一边作为开口;(a) Make a packaging pouch 8cm×11cm with a breathable water-permeable film, sew three sides, and leave one side as an opening;
(b)把大尺寸的无纺布叠放为5层用裁剪工具把5层的无纺布裁剪为小块,尺寸为6cm×9cm,每一份小块无纺布用PE膜隔开,然后叠放在一起;(b) Lay the large-sized non-woven fabric into 5 layers and cut the 5-layer non-woven fabric into small pieces with a cutting tool, the size is 6cm×9cm, and each small piece of non-woven fabric is separated by PE film, then stacked together;
(c)根据制备的量量取超纯水1000ml,利用真空泵或其他脱气装置脱去超纯水中的氧气,脱氧时间为30min;(c) measure 1000ml of ultrapure water according to the prepared amount, and use a vacuum pump or other degassing device to remove the oxygen in the ultrapure water, and the deoxygenation time is 30min;
(d)把氮气瓶连接管路后,管口接近盛放纯水的容器底部,打开气阀,流量控制在200ml/min,备用;(d) After connecting the nitrogen bottle to the pipeline, the nozzle is close to the bottom of the container containing pure water, open the air valve, and control the flow rate at 200ml/min for standby;
(e)称量试剂:用千分之一的天平称3g的L-抗坏血酸标准品;称取0.3g叶绿素标准品;用50ml的量筒量取丙二醇50ml;(e) Weighing reagents: Weigh 3g of L-ascorbic acid standard product with a one-thousandth balance; take 0.3g of chlorophyll standard product; use a 50ml graduated cylinder to measure 50ml of propylene glycol;
(f)把称量好的叶绿素用量取的50ml丙二醇溶解,然后倒入1000ml的容量瓶中,用适量(每次15ml左右)充过氮气的纯水洗涤50ml的量筒3次,洗涤液到入1000ml的容量瓶;添加称量好的L-抗坏血酸到1000ml的容量瓶中并定容至1000ml,盖盖,摇匀至L-抗坏血酸完全溶解;(f) Dissolve 50ml of propylene glycol for the amount of chlorophyll that has been weighed, then pour it into a 1000ml volumetric flask, wash the 50ml measuring cylinder 3 times with an appropriate amount (about 15ml each time) of nitrogen-filled pure water, and the washing liquid is in the inlet 1000ml volumetric flask; add the weighed L-ascorbic acid to a 1000ml volumetric flask and dilute to 1000ml, cover and shake until the L-ascorbic acid is completely dissolved;
(g)用保鲜膜隔开且叠放在一起的无纺布装入适宜的塑料自封袋中,倒入步骤(f)中的溶液(在氮气环境下操作)进行浸泡5min;(g) the non-woven fabrics separated by plastic wrap and stacked together are packed into suitable plastic ziplock bags, poured into the solution in step (f) (operated under nitrogen environment) and soaked for 5min;
(h)把浸泡好的无纺布在一定压力下挤压去掉多余的水分,然后装入步骤(a)中的袋子,并封口。(h) Squeeze the soaked non-woven fabric under a certain pressure to remove excess moisture, then put it into the bag in step (a), and seal it.
实施例2Example 2
一种保鲜效果测试用参照样,包括吸水材料,并且吸水材料内吸收有叶绿素、保湿剂和抗氧化剂的混合液,每块吸水材料由防水材料隔开,装在透气隔水袋中;A reference sample for fresh-keeping effect test, comprising a water-absorbing material, and a mixed solution of chlorophyll, a moisturizing agent and an antioxidant is absorbed in the water-absorbing material, and each water-absorbing material is separated by a waterproof material and packed in a breathable water-proof bag;
其中,抗氧化剂为L-抗坏血酸,保湿剂为丙三醇,吸水材料为无纺布,无纺布的层数为10层,尺寸为8cm×13cm,防水材料为PET膜,透气隔水袋的规格为10cm×15cm;Among them, the antioxidant is L-ascorbic acid, the moisturizing agent is glycerin, the water-absorbing material is non-woven fabric, the number of layers of the non-woven fabric is 10, the size is 8cm×13cm, the waterproof material is PET film, and the air-permeable water-proof bag is The size is 10cm×15cm;
所述保鲜效果测试用参照样的制备方法,包括以下步骤:The preparation method of the reference sample for the fresh-keeping effect test comprises the following steps:
(a)用透气隔水膜制作包装小袋10cm×15cm,缝制三边,留一边作为开口;(a) Make a packaging pouch 10cm×15cm with a breathable water-permeable film, sew three sides, and leave one side as an opening;
(b)把大尺寸的无纺布叠放为10层,用裁剪工具把10层的无纺布裁剪为小块,尺寸为8cm×13cm,每一份小块无纺布用PET膜隔开,然后叠放在一起;(b) Lay the large-sized non-woven fabric into 10 layers, cut the 10-layer non-woven fabric into small pieces with a cutting tool, the size is 8cm×13cm, and each small piece of non-woven fabric is separated by a PET film , and then stacked together;
(c)根据制备的量量取超纯水1000ml,利用真空泵或其他脱气装置脱去超纯水中的氧气,脱氧时间为60min;(c) measure 1000ml of ultrapure water according to the prepared amount, and use a vacuum pump or other degassing device to remove the oxygen in the ultrapure water, and the deoxygenation time is 60min;
(d)把氮气瓶连接管路后,管口接近盛放纯水的容器底部,打开气阀,流量控制在150ml/min,备用;(d) After connecting the nitrogen cylinder to the pipeline, the nozzle is close to the bottom of the container containing pure water, open the gas valve, and control the flow rate at 150ml/min for standby;
(e)用千分之一的天平称3.3g的L-抗坏血酸标准品;称取0.3g叶绿素标准品;用50ml的量筒量取丙三醇50ml;(e) weigh 3.3g of L-ascorbic acid standard with a one-thousandth of the balance; take 0.3g of chlorophyll standard; measure 50ml of glycerol with a 50ml graduated cylinder;
(f)把称量好的叶绿素用量取的50ml丙三醇溶解,然后倒入1000ml的容量瓶中,用适量(每次15ml左右)充过氮气的纯水洗涤50ml的量筒3次,洗涤液到入1000ml的容量瓶;添加称量好的L-抗坏血酸到1000ml的容量瓶中并定容至1000ml,盖盖,摇匀至L-抗坏血酸完全溶解;(f) Dissolve 50ml of glycerol for the weighed amount of chlorophyll, then pour it into a 1000ml volumetric flask, wash a 50ml graduated cylinder 3 times with an appropriate amount (about 15ml each) of nitrogen-filled pure water, and the washing solution into a 1000ml volumetric flask; add the weighed L-ascorbic acid to a 1000ml volumetric flask and dilute to 1000ml, cover and shake until the L-ascorbic acid is completely dissolved;
(g)用保鲜膜隔开且叠放在一起的无纺布装入适宜的塑料自封袋中,倒入步骤(f)中的溶液(在氮气环境下操作)进行浸泡8min;(g) the non-woven fabrics separated by plastic wrap and stacked together are packed into suitable plastic ziplock bags, poured into the solution in step (f) (operated under nitrogen environment) and soaked for 8min;
(h)把浸泡好的无纺布在一定压力下挤压去掉多余的液体至不滴液为止,然后装入步骤(a)中的袋子,并封口。(h) Squeeze the soaked non-woven fabric under a certain pressure to remove excess liquid until no liquid drips, then put it into the bag in step (a), and seal it.
实施例3Example 3
一种保鲜效果测试用参照样,包括吸水材料,并且吸水材料内吸收有叶绿素、保湿剂和抗氧化剂的混合液,每块吸水材料由防水材料隔开,装在透气隔水袋中;A reference sample for fresh-keeping effect test, comprising a water-absorbing material, and a mixed solution of chlorophyll, a moisturizing agent and an antioxidant is absorbed in the water-absorbing material, and each water-absorbing material is separated by a waterproof material and packed in a breathable water-proof bag;
其中,抗氧化剂为丁基羟基茴香醚,保湿剂为丙三醇,吸水材料为棉布,棉布的层数为8层,尺寸为7cm×10cm,防水材料为PE膜,透气隔水袋的规格为9cm×12cm;Among them, the antioxidant is butylated hydroxyanisole, the moisturizing agent is glycerin, the water-absorbing material is cotton, the number of layers of the cotton is 8, the size is 7cm×10cm, the waterproof material is PE film, and the specifications of the breathable water-proof bag are 9cm×12cm;
所述保鲜效果测试用参照样的制备方法,包括以下步骤:The preparation method of the reference sample for the fresh-keeping effect test comprises the following steps:
(a)用透气隔水膜制作包装小袋9cm×12cm,缝制三边,留一边作为开口;(a) Make a packaging pouch 9cm×12cm with a breathable water-permeable film, sew three sides, and leave one side as an opening;
(b)把大尺寸的棉布叠放为8层,用裁剪工具把8层的棉布裁剪为小块,尺寸为7cm×10cm,每一份小块棉布用PE膜隔开,然后叠放在一起;(b) Stack the large-sized cotton cloth into 8 layers, cut the 8-layer cotton cloth into small pieces with a cutting tool, the size is 7cm×10cm, each small piece of cotton cloth is separated by PE film, and then stacked together ;
(c)根据制备的量量取超纯水1000ml,利用真空泵或其他脱气装置脱去超纯水中的氧气,脱氧时间为50min;(c) measure 1000ml of ultrapure water according to the prepared amount, and use a vacuum pump or other degassing device to remove the oxygen in the ultrapure water, and the deoxygenation time is 50min;
(d)把氮气瓶连接管路后,管口接近盛放纯水的容器底部,打开气阀,流量控制在130ml/min,备用;(d) After connecting the nitrogen bottle to the pipeline, the nozzle is close to the bottom of the container containing pure water, open the gas valve, and control the flow rate at 130ml/min for standby;
(e)用千分之一的天平称3.3g的丁基羟基茴香醚标准品;称取0.3g叶绿素标准品;用50ml的量筒量取丙三醇50ml;(e) weigh 3.3g of butylated hydroxyanisole standard product with a one-thousandth of the balance; take 0.3g of chlorophyll standard product; measure 50ml of glycerol with a 50ml graduated cylinder;
(f)把称量好的叶绿素用量取的50ml丙三醇溶解,然后倒入1000ml的容量瓶中,用适量(每次15ml左右)充过氮气的纯水洗涤50ml的量筒3次,洗涤液到入1000ml的容量瓶;添加称量好的丁基羟基茴香醚到1000ml的容量瓶中并定容至1000ml,盖盖,摇匀至丁基羟基茴香醚完全溶解;(f) Dissolve 50ml of glycerol for the weighed amount of chlorophyll, then pour it into a 1000ml volumetric flask, wash a 50ml graduated cylinder 3 times with an appropriate amount (about 15ml each) of nitrogen-filled pure water, and the washing solution into a 1000ml volumetric flask; add the weighed butylated hydroxyanisole to a 1000ml volumetric flask and dilute to 1000ml, cover and shake until the butylated hydroxyanisole is completely dissolved;
(g)用保鲜膜隔开且叠放在一起的棉布装入适宜的塑料自封袋中,倒入步骤(f)中的溶液(在氮气环境下操作)进行浸泡8min;(g) The cotton cloths separated by plastic wrap and stacked together are packed into suitable plastic ziplock bags, poured into the solution in step (f) (operated under nitrogen environment) and soaked for 8min;
(h)把浸泡好的棉布在一定压力下挤压去掉多余的液体至不滴液为止,然后装入步骤(a)中的袋子,并封口。(h) Squeeze the soaked cotton cloth under a certain pressure to remove excess liquid until no liquid drips, then put it into the bag in step (a), and seal it.
实施例4Example 4
一种保鲜效果测试用参照样,除吸水材料使用涤纶布以外,其他操作步骤与实施例1相同。A reference sample for fresh-keeping effect test, other operation steps are the same as those in Example 1, except that polyester cloth is used as the water-absorbing material.
试验例Test example
抽样并测试实施例1-4中的保鲜效果测试用参照样中的含水、抗氧化剂和叶绿素的量,作为参照样的初始值;通过多次平行取样测试计算标准偏差或不确定度。将其放入冰箱3天后测定含水、抗氧化剂和叶绿素的量。测试结果如表一所示。Sampling and testing the amount of water, antioxidants and chlorophyll in the reference sample for the fresh-keeping effect test in Examples 1-4, as the initial value of the reference sample; standard deviation or uncertainty is calculated by multiple parallel sampling tests. The amount of water, antioxidants and chlorophyll was determined after placing it in the refrigerator for 3 days. The test results are shown in Table 1.
本发明中的保鲜效果测试用参照样包括吸水材料,吸水材料内吸收有叶绿素、保湿剂和抗氧化剂的混合液。该参照样间的一致性较好,可广泛应用于冰箱的保鲜效果评价,实验误差较小,且可重复性较好。The reference sample for the fresh-keeping effect test in the present invention includes a water-absorbing material, and the water-absorbing material absorbs a mixed solution of chlorophyll, a moisturizing agent and an antioxidant. The consistency of the reference sample is good, and it can be widely used in the evaluation of the preservation effect of refrigerators. The experimental error is small, and the repeatability is good.
最后应说明的是:以上各实施例仅用以说明本发明的技术方案,而非对其限制;尽管参照前述各实施例对本发明进行了详细的说明,本领域的普通技术人员应当理解:其依然可以对前述各实施例所记载的技术方案进行修改,或者对其中部分或者全部技术特征进行等同替换;而这些修改或者替换,并不使相应技术方案的本质脱离本发明各实施例技术方案的范围。Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: The technical solutions described in the foregoing embodiments can still be modified, or some or all of the technical features thereof can be equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the embodiments of the present invention. scope.
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