CN110039067A - A kind of preparation of electropositive gold nano seed and its application in carcinomebryonic antigen detection - Google Patents
A kind of preparation of electropositive gold nano seed and its application in carcinomebryonic antigen detection Download PDFInfo
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- CN110039067A CN110039067A CN201910389358.0A CN201910389358A CN110039067A CN 110039067 A CN110039067 A CN 110039067A CN 201910389358 A CN201910389358 A CN 201910389358A CN 110039067 A CN110039067 A CN 110039067A
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 70
- 239000010931 gold Substances 0.000 title claims abstract description 68
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 68
- 239000000427 antigen Substances 0.000 title claims abstract description 29
- 102000036639 antigens Human genes 0.000 title claims abstract description 29
- 108091007433 antigens Proteins 0.000 title claims abstract description 29
- 238000001514 detection method Methods 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 239000000243 solution Substances 0.000 claims abstract description 16
- 239000007864 aqueous solution Substances 0.000 claims abstract description 14
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 229910000033 sodium borohydride Inorganic materials 0.000 claims abstract description 7
- 239000012279 sodium borohydride Substances 0.000 claims abstract description 7
- 239000005457 ice water Substances 0.000 claims abstract description 5
- 239000011259 mixed solution Substances 0.000 claims abstract description 4
- 238000009835 boiling Methods 0.000 claims abstract description 3
- 238000010438 heat treatment Methods 0.000 claims abstract description 3
- 238000004062 sedimentation Methods 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 2
- DCAYPVUWAIABOU-UHFFFAOYSA-N hexadecane Chemical compound CCCCCCCCCCCCCCCC DCAYPVUWAIABOU-UHFFFAOYSA-N 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 1
- AISMNBXOJRHCIA-UHFFFAOYSA-N trimethylazanium;bromide Chemical compound Br.CN(C)C AISMNBXOJRHCIA-UHFFFAOYSA-N 0.000 claims 1
- 239000000439 tumor marker Substances 0.000 abstract description 5
- 239000002245 particle Substances 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 238000003745 diagnosis Methods 0.000 abstract 1
- 239000002105 nanoparticle Substances 0.000 description 14
- 238000010521 absorption reaction Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 12
- 239000000523 sample Substances 0.000 description 7
- 201000011510 cancer Diseases 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 229910021645 metal ion Inorganic materials 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 238000013399 early diagnosis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- -1 salt ion Chemical class 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical compound O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 239000002122 magnetic nanoparticle Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002114 nanocomposite Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 201000001275 rectum cancer Diseases 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
- B22F2009/245—Reduction reaction in an Ionic Liquid [IL]
Abstract
The invention discloses a kind of preparation of electropositive gold nano seed and its applications in carcinomebryonic antigen detection, preparation method is: being first uniformly mixed cetyl trimethylammonium bromide aqueous solution, aqueous solution of chloraurate at normal temperature, then the sodium borohydride ice water solution of 0.1mol/L is added dropwise into above-mentioned mixed solution while stirring in a heated state, it is gradually warmed up until boiling, it is further continued for heating stirring to final solution color and becomes claret, obtain electropositive gold nano seed；The concentration of ultraviolet absorptivity and carcinomebryonic antigen of the gold nano seed at 526nm is in a linear relationship in 500pg/mL to 10ng/mL range.Electropositive gold nano seed particle size is between 15-20nm prepared by the present invention, preparation method is simple, good biocompatibility, and stability is good, it can be with the quick specific adsorption of carcinomebryonic antigen, the early detection that can effectively realize tumor marker, has a good application prospect in terms of the diagnosis of disease and prevention.
Description
Technical field
The present invention relates to tumor marker detection technique fields, and in particular to a kind of preparation of electropositive gold nano seed and
Its application in carcinomebryonic antigen detection
Background technique
Carcinomebryonic antigen (carcinoembryonic antigen, CEA) be nineteen sixty-five by Gold and Freedman first from
A kind of tumor associated antigen extracted in colon cancer and embryonic tissue, the specificity as diagnosing colon and the carcinoma of the rectum is marked in early days
Will object, through a large amount of clinical practice, the malignant tumour CEA value of discovery not only gastrointestinal tract can be increased, breast cancer, lung cancer and its
Also there is raising in the serum of his malignant tumour.Therefore, carcinomebryonic antigen can be used as a kind of broad-spectrum tumor marker.
The common detection of carcinomebryonic antigen has immunoturbidimetry and enzyme linked immunosorbent assay etc..Since enzyme linked immunosorbent assay is examined
Survey time-consuming and complicated for operation, be no longer satisfied the requirement of large hospital rapid quantitative detection, and immunoturbidimetry with it is complete from
Dynamic biochemical instruments are popularized, and are had been developed that and are carried out a variety of tachysynthesises than turbid detection technique.
With optical nano technology being constantly progressive in terms of bio-sensing research and development, nano material such as: gold nanoparticle, silver
Nanoparticle, magnetic nano-particle and composite nanoparticle etc. become the emphasis of researchers' concern.Wherein, gold nanoparticle by
In good biocompatibility, biggish reference area and become one of the emphasis of researchers' concern.It is existing to use gold nanoparticle
For probe colorimetric method sensing technology mainly by elecrtonegativity gold nanoparticle (Chemical Reviews, 2012,112,
2739-2779) dispersion caused by after, being added by the elecrtonegativity gold nanoparticle of observation label or non-marked due to target molecule
State changes and adjoint color change, and the positional shift by monitoring ultraviolet absorption peak realizes the quantitative detection of target molecule.
Elecrtonegativity gold nanoparticle used in such method will lead to Jenner's grain of rice to media environment poor resistance, high ionic strength
Son aggregation, to restrict the practical application of such method.Compared with gold nanoparticle, gold nano of the gold nanorods than same volume
Particle is absorbing and high an order of magnitude on scattering section；And gold nanorods are also high to the sensibility of ambient enviroment variations in refractive index
In gold nanoparticle (Journal of American Chemical Society, 2008,130,2780-2782), therefore
Also there is more application in sensing technology.However, these are by observation gold nanoparticle or nanometer rods color change or ultraviolet suction
The sensing technology sensitivity for receiving peak position offset is lower.Therefore, seek easier, quick, sensitive immunoturbidimetry detection side
Method is very necessary.
Summary of the invention
An object of the present invention is to provide a kind of preparation method of electropositive gold nano seed, and it is good can to obtain stability
Good electropositive gold nano seed.
The second object of the present invention is to provide electropositive gold nano seed prepared by the above method to detect in carcinomebryonic antigen
In application.
To achieve the above object, The technical solution adopted by the invention is as follows: a kind of preparation side of electropositive gold nano seed
Method, comprising the following steps:
(1) the cetyl trimethylammonium bromide aqueous solution and concentration that compound concentration is 0.01mol/L respectively are
The aqueous solution of chloraurate of 0.001mol/L；
(2) cetyl trimethylammonium bromide aqueous solution, aqueous solution of chloraurate are pipetted respectively, are stirred under room temperature
It is even；
(3) the sodium borohydride ice water of 0.1mol/L is added dropwise into above-mentioned mixed solution while stirring in a heated state
Solution, when dropwise addition, is gradually warmed up until boiling, and continues heating stirring to solution colour under fluidized state and become claret, i.e.,
Electropositive gold nano seed is made.
Preferably, the cetyl trimethylammonium bromide aqueous solution, aqueous solution of chloraurate and sodium borohydride ice water solution
Volume ratio be 2:15:1.8.
Method also provides for application of the above-mentioned gold nano seed in carcinomebryonic antigen quantitative detection.It comprises the steps of:
It takes 500 μ L electropositive gold nano seeds in centrifuge tube, the 10 μ L of cancer embryo antibody of fixed concentration is added into centrifuge tube, be incubated for one
The dispersity situation of gold nanoparticle and the situation of change of ultraviolet absorption peak are observed after fixing time；Then, with constant concentration
Bovine serum albumin(BSA) closing；Then the carcinomebryonic antigen of concentration gradient change from low to high is added, observes the sedimentation of gold nano seed
Decline situation with ultraviolet absorption peak, realizes the rapid sensitive detection of carcinomebryonic antigen.
Experiment is it is found that the ultraviolet absorptivity sedimentation situation of the electropositive gold nano seed and the concentration of carcinomebryonic antigen exist
It is in good linear relationship in 500pg/mL to 10ng/mL range.
Compared with prior art, the electropositive gold nano seed particle size prepared by the present invention is between 15-20nm, the nanometer
Seed good biocompatibility and have good optical characteristics.Due to prepared electropositive gold nano seed ctab surface function
Change and there is it in the complex environment containing high salt concentration ion, high concentration albumen, mixed metal ion and zwitterion
Good stability can be such that the immunological probe is buffering to improve electropositive gold nano seed to media environment tolerance
It is identified in a variety of media such as solution and serum, the gold nano immunological probe of this method preparation can be quick with carcinomebryonic antigen
Specific adsorption promotes global density to increase and settle, and then passes through and reads the decline of gold nano-probe absorbance with carcinomebryonic antigen
The case where concentration increases, realizes the early stage quantitative detection of tumor marker.This method and tradition are using gold nanoparticle as probe
Colorimetric method is compared, and observation nano-probe color change and ultraviolet absorption peak offset are not needed, as long as there is the combination of nanoparticle heavy
Drop and the decline of adjoint ultraviolet absorption peak can be detected, and largely improve the sensitivity of detection, anti-to cancer embryo in serum
Original detection can achieve pg/mL level, have a good application prospect in terms of the early diagnosis of disease and prevention.
Detailed description of the invention
Fig. 1 is the transmission electron microscope picture of electropositive gold nano seeded dispersion state prepared by the present invention；
Fig. 2 is the zeta potential diagram of electropositive gold nano seed prepared by the present invention；
Fig. 3 is the study on the stability figure of electropositive gold nano seed prepared by the present invention, A: salt ion；B: protein；C:
Mixed metal ion and zwitterion；
Fig. 4 is electropositive gold nano seed prepared by the present invention to carcinomebryonic antigen response diagram, A: sedimentation pictorial diagram；B: purple
External spectrum figure；C: linear relationship chart；
Fig. 5 is the method for the present invention and conventional enzyme-linked immunization tumor marker testing result comparison diagram.
Specific embodiment
Invention is further described in detail in the following with reference to the drawings and specific embodiments.
The preparation of 1. electropositive gold nano seed of embodiment and study on the stability
The preparation of 1.1 electropositive gold nano seeds
It weighs 0.0364g cetyl trimethylammonium bromide (CTAB) first to be dissolved in the ultrapure water of 10mL, at 30 DEG C
Under the conditions of dissolve CTAB；It weighs 0.03938g gold chloride to be dissolved in 10mL ultrapure water, all 10 times of dilution is spare after dissolution；Claim
0.0378g sodium borohydride is taken to be dissolved in 10mL mixture of ice and water.2mL CTAB solution and 15mL chlorauric acid solution are pipetted in glass
In beaker, the stirring at normal temperature on magnetic stirring apparatus starts heating water bath and is gradually heated to boil, heats the phase after stirring 15 minutes
Between 1.8mL sodium borohydride solution is added dropwise, and continue under fluidized state to heat magnetic agitation to solution colour to become wine red
Color obtains electropositive gold nano seed.Meanwhile the dispersity of the gold nano seed, such as Fig. 1 are investigated by transmission electron microscope
Shown, prepared gold nano seed average grain diameter is between 14-18nm；The electricity of the gold nano seed is tested by particle instrument
Position, as shown in Fig. 2, analysis, which can obtain institute's gold nano seed, shows electropositive, current potential is+39.8 mV.
The study on the stability of 1.2 electropositive gold nano seeds
The centrifuge tube of 3 1.5mL is taken, is separately added into the electropositive gold nano seed of the 500 above-mentioned preparations of μ L thereto, then
After being separately added into the NaCl of 0,100mmol/L, 500mmol/L into 3 centrifuge tubes, the electropositive gold nano of above-mentioned preparation is observed
Seeded dispersion state and ultraviolet absorption peak variation, as shown in Figure 3A, which is containing high salt concentration ion
Solution in dispersity, color and ultraviolet absorption peak there is no significant change.
The centrifuge tube of 3 1.5mL is taken, is separately added into the electropositive gold nano seed of the 500 above-mentioned preparations of μ L thereto, then
After being separately added into the bovine serum albumin(BSA) of 0,10mg/mL, 50mg/mL into 3 centrifuge tubes, the electropositive gold of above-mentioned preparation is observed
The dispersity and ultraviolet absorption peak variation of nanometer seed, as shown in Fig. 3 B, which is containing high concentration
Dispersity, color and ultraviolet absorption peak do not have significant change in the solution of protein.
The centrifuge tube of 3 1.5mL is taken, is separately added into the electropositive gold nano seed of the 500 above-mentioned preparations of μ L thereto, then
The K of 0,100mmol/L, 500mmol/L is separately added into 3 centrifuge tubes+,Ca2+,Mg2+, NO3-,NH4 +Afterwards, above-mentioned system is observed
The dispersity and ultraviolet absorption peak of standby electropositive gold nano seed change, as shown in Figure 3 C, the electropositive gold nano seed
Dispersity, color and ultraviolet absorption peak obviously do not become in containing the solution after mixed metal ion and conventional zwitterion
Change.
The above results sufficiently demonstrate high stability of the gold nano seed in mixed solution.
2. electropositive gold nano seed of embodiment is used for the quantitative detection of carcinomebryonic antigen
The centrifuge tube of 7 1.5mL is taken first, is separately added into the electropositive gold nano seed of the 500 above-mentioned preparations of μ L thereto,
The cancer embryo antibody and room temperature incubation 5 minutes that 10 μ L concentration are 50 μ g/mL are added into centrifuge tube from the sequence of serial number 1 to 7, then
10 μ L bovine serum albumin(BSA)s closing (0.25%) is added, states concentration change of gradient from low to high is added in centrifuge tube then up
Carcinomebryonic antigen (concentration is respectively 300pg/mL, 500 pg/mL, 1.5ng/mL, 3.0ng/mL, 5.0ng/mL, 8.0ng/mL,
10ng/mL), and after room temperature incubation 2 hours, the situation of change of gold nano seeded dispersion state and ultraviolet absorption peak is observed.Such as figure
Shown in 4A, the dispersity of electropositive gold nano seed is more and more as the increase of carcinomebryonic antigen concentration settles.Such as Fig. 4 B institute
Show, the ultraviolet absorption peak of corresponding gold nano seed is also gradually reduced, and is arrived with the concentration of carcinomebryonic antigen in 500 pg/mL
Good linear relationship, coefficient R are presented within the scope of 10ng/mL2=0.9912 (as shown in Fig. 4 C).Therefore, it can be based on
The quantitative detection of carcinomebryonic antigen is effectively realized in the sedimentation of electropositive gold nano seed.
Present invention also provides be that probe carries out carcinomebryonic antigen detection and conventional enzyme linked immunological using above-mentioned gold nano seed
The comparative result figure of method detection, as shown in Figure 5, the results showed that this method does not surpass with conventional enzyme-linked immune detection method resultant error
Cross 5%.It follows that electropositive gold nano seed application can improve carcinomebryonic antigen inspection well in the detection of carcinomebryonic antigen
The specificity of survey, simplifies experimental procedure, has broad application prospects in terms of the early diagnosis of disease and prevention.
Claims (4)
1. a kind of preparation method of electropositive gold nano seed, which comprises the following steps:
(1) the cetyl trimethylammonium bromide aqueous solution and concentration that compound concentration is 0.01mol/L respectively are 0.001mol/L
Aqueous solution of chloraurate；
(2) cetyl trimethylammonium bromide aqueous solution, aqueous solution of chloraurate are pipetted respectively, are uniformly mixed under room temperature；
(3) the sodium borohydride ice water that 0.1mol/L is added dropwise into above-mentioned mixed solution while stirring in a heated state is molten
Liquid, when dropwise addition, is gradually warmed up until boiling, and continues heating stirring to solution colour under fluidized state and become claret, that is, makes
Obtain electropositive gold nano seed.
2. a kind of preparation method of electropositive gold nano seed according to claim 1, which is characterized in that the hexadecane
The volume ratio of base trimethylammonium bromide aqueous solution, aqueous solution of chloraurate and sodium borohydride ice water solution is 2:15:1.8.
3. application of the electropositive gold nano seed made from preparation method of any of claims 1 or 2 in carcinomebryonic antigen detection.
4. application of the electropositive gold nano seed according to claim 3 in carcinomebryonic antigen detection, which is characterized in that institute
State electropositive gold nano seed at 526nm the sedimentation of ultraviolet absorptivity and the concentration of carcinomebryonic antigen in 500pg/mL to 10ng/
It is in good linear relationship within the scope of mL.
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