CN110038152A - A kind of preparation method for the gelatine nano fiber hemostatic material can promote platelet aggregation - Google Patents
A kind of preparation method for the gelatine nano fiber hemostatic material can promote platelet aggregation Download PDFInfo
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- CN110038152A CN110038152A CN201910135857.7A CN201910135857A CN110038152A CN 110038152 A CN110038152 A CN 110038152A CN 201910135857 A CN201910135857 A CN 201910135857A CN 110038152 A CN110038152 A CN 110038152A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0036—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0042—Materials resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/104—Gelatin
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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Abstract
The invention discloses a kind of preparation methods of gelatine nano fiber hemostatic material that can promote platelet aggregation, include the following steps: step S1: the gelatin solution of swelling；Step S2: dissolved gelatin solution is obtained；Step S3: the gelatin solution after obtaining spinning；Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported under the action of high voltage electric field E2 and is polarised again；Step S5: being iteratively repeated step S4, until gelatin fiber film reaches certain thickness；Step S6: gelatine nano fiber hemostatic material is obtained.The present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, and the haemostatic effect of the gelatine nano fiber hemostatic material of unit volume of the invention is good, and the infection probability of wound location is small, and the present invention has good biocompatibility and biodegradability.
Description
Technical field
The present invention relates to a kind of preparation methods of gelatine nano fiber hemostatic material that can promote platelet aggregation.
Background technique
About 4000~6000 milliliters of total blood volume of human body, in emergencies such as surgical operation, traffic accident, natural calamity and wars
Under, when acute bleeding is more than 800~1000 milliliters, it will be in peril of one's life.Therefore, emergency survival hemostatic material is developed, is striven for
Time is that the wounded effectively stop blooding in time, is had very important significance to its life is saved.Traditional gauze, bandage and cotton
The hemostatic materials such as yarn there are haemostatic effects undesirable, nonabsorable easily causes rejection to be infected and the shortcomings that other complication, can not
Meets the needs of quick hemostasis, although and novel hemostatic gauze and styptic sponge is improved on haemostatic effect, drop
Solution performance is poor, and demolishing process often will cause the secondary injury of patient.With the development of science and technology, novel absorbable hemostatic
Material is shown one's talent, and is not only play an important role on the market, and the research hotspot in terms of medical material is more become.
At present in Novel absorbable hemostatic material, including cellulose family, chitosan class, starch, colloidal type and other productions
Five major class of product.Gelatin has good biocompatibility, degradable and absorbability, absorbent as colloidal type hemostatic material
Well, the advantages of can promote platelet activation and sludged blood formation, has been applied to nosebleed, neurosurgery and Urology Surgery etc.
In operation, however there is the risk for increasing wound site infection rate, therefore such as more than after doses in gelatin hemostatic material dosage
The haemostatic effect what improves gelatin hemostatic material is the key that the such material application of developing.
Relative to gelfoam hemostatic material, the material specific surface area of nanofibrous structures is bigger, the contact surface with blood
Product is more, and blood platelet is easier to adhesion and aggregation and improves haemostatic effect in fiber surface so as to shorten bleeding stopping period.Electrostatic spinning is
A kind of nanofiber technology of preparing of simple process, while the presence of its high-voltage electrostatic field, also for polar material provide polarization and
Capturing charge offer may.Most protein such as collagen etc. have can store for a long time real charge and keep polarization shape
The ability of state.Gelatin is as the product after collagen hydro, molecular chain structure and collagen and its similar, in the effect of high voltage electric field
Under, the charged groups such as carboxyl, amino tend to orderly polarization offset, and fetter the charge intensified in a large amount of electric fields, make fiber shape
The continuous positive electricity clearance channel at countless tiny.The presence of nanofiber, the especially topological structure of fiber surface simultaneously
The adherency of blood platelet is had a very big impact with surface charge, but existing electrostatic spinning, it often introduces organic solvent and makees
For spinning solution solvent, to cause the risk of infected wound there are dissolvent residual.Existing also useful water do solvent be prepared for it is pure
Gelatin electrospun fiber membrane, but the yield of spinning is humble, and the case where there are capillary blockages, it is unfavorable for the application of industrialization.
Summary of the invention
The purpose of the present invention is overcoming deficiency in the prior art, a kind of gelatin nanometer that can promote platelet aggregation is provided
The preparation method of fiber hemostatic material.
In order to achieve the above object, the present invention is achieved by the following technical solutions:
A kind of preparation method for the gelatine nano fiber hemostatic material can promote platelet aggregation, includes the following steps:
Step S1: gelatin being placed in solvent and is dissolved, and obtains gelatin solution, gelatin solution is then placed in container
Middle room temperature is swollen 20-40min, so that gelatin chains are opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 40-70 DEG C is dissolved into 10-60min, after obtaining dissolution
Gelatin solution；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, electrostatic spinning is then carried out, to obtain spinning
Gelatin solution afterwards；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
It is polarised under the action of piezoelectric field E2 and polarization parameter is set；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches certain thickness；
Step S6: gelatin fiber film is removed, is then dried in a vacuum drying oven, is then packed with sterilizing bag, then
Sterilizing, then in shady and cool kept dry, to obtain gelatine nano fiber hemostatic material.
Preferably, the certain thickness is between 0.1-10mm.
Preferably, the mass percent of the gelatin in institute's gelatine solution is between 5%-25%, solvent is deionization
Water or pure water or high purity water.
Preferably, it is voltage 15kv- between 40 DEG C -70 DEG C that the parameter of the spinning solution storage device, which is temperature,
Between 80kv, electric current is between 0mA-0.5mA, and spinning distance is between 5cm-30cm.
Preferably, the indoor environment temperature is the revolving speed 1r/min-10r/min of the lace curtaining between 30 DEG C -60 DEG C
Between.
Preferably, the polarization parameter, between voltage 30kv-70kv, electric current is between 1mA-10mA, and polarize distance
Between 5cm-30cm.
Preferably, the temperature dried in a vacuum drying oven is between 35 DEG C -80 DEG C, the dry time is 2h-
Between 48h.
Preferably, the method for the electrostatic spinning is spiral textile or needle spinning or metal silk spinning or sheet metal is spun or metal is convex
Point is spun.
Preferably, the method for the polarization process be corona polarizing or thermal poling or the polarization of soft X-ray or it is radiation-polarizing or
Magnetic polarization, the method for the sterilizing are ethylene oxide sterilizing or ultraviolet sterilization or radiosterilization.
Preferably, institute's gelatine solution is that edible gelatin solution is or medical gelatin solution or pharmagel solution.
Beneficial effects of the present invention are as follows: the present invention by the setting parameter of spinning solution storage device, indoor environment temperature,
The revolving speed and polarization parameter of lace curtaining, and then control the microstructure and polarization performance of gelatin fiber film, gelatin nanometer of the invention
Fiber hemostatic material porous surface, hydrophilic absorbency is high, so that the present invention has, hydrophilic absorbency is good, platelet adhesion rate
The haemostatic effect of high advantage, the gelatine nano fiber hemostatic material of unit volume of the invention is good, the infection machine of wound location
Rate is small, and it is a kind of Novel absorbable hemostatic material that the present invention, which has good biocompatibility and biodegradability, the present invention
Without the use of any chemical reagents during preparing gelatine nano fiber hemostatic material, and simple production process, the present invention are
A kind of preparation method of Environmental Safety.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of gelatine nano fiber hemostatic material prepared by the present invention.
Specific embodiment
Technical solution of the present invention is described further below:
Embodiment 1:
A kind of preparation method for the gelatine nano fiber hemostatic material can promote platelet aggregation, includes the following steps:
Step S1: gelatin being placed in solvent and is dissolved, and obtains gelatin solution, gelatin solution is then placed in container
Middle room temperature is swollen 20-40min, so that gelatin chains be made to open, the gelatin solution being swollen is bright in institute's gelatine solution
For the mass percent of glue between 5%-25%, solvent is deionized water or pure water or high purity water；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 40-70 DEG C is dissolved into 10-60min, after obtaining dissolution
Gelatin solution so that gelatin chains unordered helical form curling, hydrophilic radical is controllably unfolded on strand；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, electrostatic spinning is then carried out, to obtain spinning
Gelatin solution afterwards, the parameter of the spinning solution storage device are that temperature is between 40 DEG C -70 DEG C, voltage be 15kv-80kv it
Between, electric current between 0mA-0.5mA, spinning distance for 5cm-30cm between, the indoor environment temperature for 30 DEG C -60 DEG C it
Between, between the revolving speed 1r/min-10r/min of the lace curtaining；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
Polarization parameter is polarised and is arranged under the action of piezoelectric field E2, and the polarization parameter is between voltage 30kv-70kv, electricity
Stream is between 1mA-10mA, and polarization distance is between 5cm-30cm；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches 0.1-10mm；
Step S6: gelatin fiber film is removed, is then dried in a vacuum drying oven, is then packed with sterilizing bag, then
Sterilizing, then in shady and cool kept dry, to obtain gelatine nano fiber hemostatic material.The drying in a vacuum drying oven
Temperature is between 35 DEG C -80 DEG C, and the dry time is between 2h-48h.
The method of the electrostatic spinning is spiral textile or needle spinning or metal silk spinning or sheet metal is spun or metal salient point is spun.It is described
The method of polarization process is corona polarizing or thermal poling or the polarization of soft X-ray or radiation-polarizing or magnetic polarization, the method for the sterilizing
For ethylene oxide sterilizing or ultraviolet sterilization or radiosterilization.Institute's gelatine solution is that edible gelatin solution is or medical gelatin solution
Or pharmagel solution.
The present invention provides a kind of preparation methods of gelatine nano fiber hemostatic material that can promote platelet aggregation, such as scheme
Shown in 1, micro-optic structure is controllable, by the gelatin solution after spinning under the action of high voltage electric field E1, ejects from spinning axis
Liquid jet, and gradually stretch and attenuate, while by parameter, the indoor environment temperature, lace curtaining for adjusting spinning solution storage device
Revolving speed and polarization parameter, gelatin hydrophilic radical and gelatin chains can be effectively controlled before jet stream is fully cured unfolds journey
Degree.The coordinated regulation of voltage and temperature can not only be such that the molecular chain orientation inside gelatin fiber defines, arrange close, Er Qieke
Promote gelatin chains to unfold, and then makes the hydrophilic radical of fiber surface integrated distribution multiple functions.When passing through electric field E2,
Effective orientation texture of gelatin fiber inner part subchain is more likely formed polarization charge or dipole, the charged groups such as carboxyl and amino
Also it can tend to orderly polarization offset, and adsorb the charge intensified in a large amount of electric fields, form intrastitial space bound charge, make
Fiber forms countless tiny and continuous positive electricity clearance channel, so that the adherency for significantly improving blood platelet in fiber film surface is special
Property.And the aggregation of blood platelet leads to the quick cohesion of invasive blood, and it is significant in hemostatic material field to assign gelatine nano fiber
Application advantage.
The present invention by the setting parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed and polarization parameter,
And then the microstructure and polarization performance of gelatin fiber film are controlled, gelatine nano fiber hemostatic material porous surface of the invention,
Hydrophilic absorbency is high, so that the present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, unit of the invention
The haemostatic effect of the gelatine nano fiber hemostatic material of volume is good, and the infection probability of wound location is small, and the present invention has good
Biocompatibility and biodegradability, are a kind of Novel absorbable hemostatic materials, and the present invention stops preparing gelatine nano fiber
Without the use of any chemical reagents in blood materials process, and simple production process, the present invention are a kind of preparation sides of Environmental Safety
Method.
Embodiment 2:
The preparation method that can promote the gelatine nano fiber hemostatic material of platelet aggregation, includes the following steps:
Step S1: it weighs 20g ox bone gelatin and is added in 180g deionized water, be swollen 20min at normal temperature, to make bright
Xanthan molecule chain is opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 60 DEG C is dissolved into 30min, obtains dissolved gelatin
Solution, so that the unordered helical form of gelatin chains crimps, hydrophilic radical is controllably unfolded on strand；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, then carried out using spiral electrostatic spinning mode quiet
Electrospun, to obtain the gelatin solution after spinning, the parameter of the spinning solution storage device be temperature be 40 DEG C -45 DEG C it
Between, voltage is between 15kv-80kv, and electric current is between 0mA-0.5mA, and spinning distance is between 5cm-30cm, the interior ring
Border temperature is the revolving speed 10r/min of the lace curtaining between 30 DEG C -35 DEG C；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
Polarization parameter is polarised and is arranged under the action of piezoelectric field E2, and the mode of polarization process is Filamentous corona polarizing, described
Polarization parameter is voltage 60kv, electric current 3mA, and polarization distance is 10cm；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches 0.1-10mm；
Step S6: gelatin fiber film is removed, and in 40 DEG C of vacuum ovens after drying for 24 hours, is sealed with sterilizing bag, epoxy
Ethane sterilizing is placed on 4 DEG C of preservations, to obtain gelatine nano fiber hemostatic material.
As shown in Figure 1, detecting spinning fibre diameter by instrument is in normal distribution, main distribution is 300-
1200nm takes gelatine nano fiber hemostatic material 0.05g of the invention, existing market control sample 0.05g and blank sample, according to
Pooled plasma 5.0mL is added in the ratio of 0.01g/mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates
2h, with the platelet concentration in Full automatic animal cellanalyzer measurement blood, measurement result are as follows: blank group 199.3 ± 2.9
× 109/L, market samples control group be 174.3 ± 10.0 × 109/L, gelatine nano fiber hemostatic material be 131.0 ±
1.0 × 109/L, there were significant differences between three.It will thus be seen that material surface of the present invention is porous, hydrophilic absorbency is high, tool
There is excellent platelet adhering function.
The present invention by the setting parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed and polarization parameter,
And then the microstructure and polarization performance of gelatin fiber film are controlled, gelatine nano fiber hemostatic material porous surface of the invention,
Hydrophilic absorbency is high, so that the present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, unit of the invention
The haemostatic effect of the gelatine nano fiber hemostatic material of volume is good, and the infection probability of wound location is small, and the present invention has good
Biocompatibility and biodegradability, are a kind of Novel absorbable hemostatic materials, and the present invention stops preparing gelatine nano fiber
Without the use of any chemical reagents in blood materials process, and simple production process, the present invention are a kind of preparation sides of Environmental Safety
Method.
Embodiment 3:
The preparation method that can promote the gelatine nano fiber hemostatic material of platelet aggregation, includes the following steps:
Step S1: it weighs 10g ox bone gelatin and is added in 190g deionized water, be swollen 20min at normal temperature, to make bright
Xanthan molecule chain is opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 55 DEG C is dissolved into 30min, obtains dissolved gelatin
Solution, so that the unordered helical form of gelatin chains crimps, hydrophilic radical is controllably unfolded on strand；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, then carried out using spiral electrostatic spinning mode quiet
Electrospun, to obtain the gelatin solution after spinning, the parameter of the spinning solution storage device be temperature be 40 DEG C -45 DEG C it
Between, voltage 80kv, electric current between 0.3mA, spinning distance be 10cm, the indoor environment temperature be 30 DEG C -36 DEG C between,
The revolving speed 10r/min of the lace curtaining；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
Polarization parameter is polarised and is arranged under the action of piezoelectric field E2, and the mode of polarization process is Filamentous corona polarizing, described
Polarization parameter is voltage 60kv, electric current 3mA, and polarization distance is 10cm；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches 0.1-10mm；
Step S6: gelatin fiber film is removed, and in 40 DEG C of vacuum ovens after drying for 24 hours, is sealed with sterilizing bag, epoxy
Ethane sterilizing is placed on 4 DEG C of preservations, to obtain gelatine nano fiber hemostatic material.
As shown in Figure 1, detecting spinning fibre diameter by instrument is in normal distribution, main distribution is 300-870nm.
Gelatine nano fiber hemostatic material 0.05g of the invention, existing market control sample 0.05g and blank sample are taken, according to
Pooled plasma 5.0mL is added in the ratio of 0.01g/mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates
2h, with the platelet concentration in Full automatic animal cellanalyzer measurement blood.Measurement result are as follows: blank group 199.3 ± 2.9
×109A/L, market samples control group are 174.3 ± 10.0 × 109A/L, gelatine nano fiber hemostatic material be 127.1 ±
1.0×109A/L, there were significant differences between three.
It will thus be seen that material surface of the present invention is porous, hydrophilic absorbency is high, has excellent platelet adhering function.
The present invention by the setting parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed and polarization parameter,
And then the microstructure and polarization performance of gelatin fiber film are controlled, gelatine nano fiber hemostatic material porous surface of the invention,
Hydrophilic absorbency is high, so that the present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, unit of the invention
The haemostatic effect of the gelatine nano fiber hemostatic material of volume is good, and the infection probability of wound location is small, and the present invention has good
Biocompatibility and biodegradability, are a kind of Novel absorbable hemostatic materials, and the present invention stops preparing gelatine nano fiber
Without the use of any chemical reagents in blood materials process, and simple production process, the present invention are a kind of preparation sides of Environmental Safety
Method.
Embodiment 4:
The preparation method that can promote the gelatine nano fiber hemostatic material of platelet aggregation, includes the following steps:
Step S1: it weighs 75g ox bone gelatin and is added in 225g deionized water, be swollen 20min at normal temperature, to make bright
Xanthan molecule chain is opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 60 DEG C is dissolved into 40min, obtains dissolved gelatin
Solution, so that the unordered helical form of gelatin chains crimps, hydrophilic radical is controllably unfolded on strand；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, then carried out using spiral electrostatic spinning mode quiet
Electrospun, to obtain the gelatin solution after spinning, the parameter of the spinning solution storage device be temperature be 40 DEG C -45 DEG C it
Between, voltage 80kv, electric current 0.31mA, spinning distance are 17cm, and the indoor environment temperature is institute between 30 DEG C -34 DEG C
State the revolving speed 10r/min of lace curtaining；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
Polarization parameter is polarised and is arranged under the action of piezoelectric field E2, and the mode of polarization process is Filamentous corona polarizing, described
Polarization parameter is voltage 60kv, electric current 3mA, and polarization distance is 10cm；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches 0.1-10mm；
Step S6: gelatin fiber film is removed, and in 40 DEG C of vacuum ovens after drying for 24 hours, is sealed with sterilizing bag, epoxy
Ethane sterilizing is placed on 4 DEG C of preservations, to obtain gelatine nano fiber hemostatic material.
As shown in Figure 1, detecting spinning fibre diameter by instrument is in normal distribution, main distribution is 500-
1500nm。
Gelatine nano fiber hemostatic material 0.05g of the invention, existing market control sample 0.05g and blank sample are taken, according to
Pooled plasma 5.0mL is added in the ratio of 0.01g/mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates
2h, with the platelet concentration in Full automatic animal cellanalyzer measurement blood.Measurement result are as follows: blank group 199.3 ± 2.9
×109A/L, market samples control group are 174.3 ± 10.0 × 109A/L, gelatine nano fiber hemostatic material be 132.3 ±
1.2×109A/L, there were significant differences between three.It will thus be seen that material surface of the present invention is porous, hydrophilic absorbency is high, tool
There is excellent platelet adhering function.
The present invention by the setting parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed and polarization parameter,
And then the microstructure and polarization performance of gelatin fiber film are controlled, gelatine nano fiber hemostatic material porous surface of the invention,
Hydrophilic absorbency is high, so that the present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, unit of the invention
The haemostatic effect of the gelatine nano fiber hemostatic material of volume is good, and the infection probability of wound location is small, and the present invention has good
Biocompatibility and biodegradability, are a kind of Novel absorbable hemostatic materials, and the present invention stops preparing gelatine nano fiber
Without the use of any chemical reagents in blood materials process, and simple production process, the present invention are a kind of preparation sides of Environmental Safety
Method.
Embodiment 5:
The preparation method that can promote the gelatine nano fiber hemostatic material of platelet aggregation, includes the following steps:
Step S1: it weighs 25g sheep bone gelatin and is added in 175g deionized water, be swollen 20min at normal temperature, to make bright
Xanthan molecule chain is opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 60 DEG C is dissolved into 30min, obtains dissolved gelatin
Solution, so that the unordered helical form of gelatin chains crimps, hydrophilic radical is controllably unfolded on strand；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, then carried out using spiral electrostatic spinning mode quiet
Electrospun, to obtain the gelatin solution after spinning, the parameter of the spinning solution storage device is that temperature is between 38-43 DEG C,
Voltage is 25kv, and electric current 0.03mA, spinning distance is 10cm, and the indoor environment temperature is the net between 37 DEG C -38 DEG C
The revolving speed 12r/min of curtain；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
Polarization parameter is polarised and is arranged under the action of piezoelectric field E2, and the mode of polarization process is the polarization of soft X-ray, the polarization
Parameter is voltage 60kv, electric current 3mA, and polarization distance is 10cm, vertical irradiation intensity 10keV；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches 0.1-10mm；
Step S6: gelatin fiber film is removed, and in 40 DEG C of vacuum ovens after drying for 24 hours, is sealed with sterilizing bag, epoxy
Ethane sterilizing is placed on 4 DEG C of preservations, to obtain gelatine nano fiber hemostatic material.
As shown in Figure 1, detecting spinning fibre diameter by instrument is in normal distribution, main distribution is 400-960nm.
Gelatine nano fiber hemostatic material 0.10g of the invention, existing market control sample 0.10g and blank sample are taken, according to
Pooled plasma 5.0mL is added in the ratio of 0.02g/mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates
2h, with the platelet concentration in Full automatic animal cellanalyzer measurement blood.Measurement result are as follows: blank group 199.3 ± 2.9
×109A/L, market samples control group are 159.3 ± 14.4 × 109A/L, gelatine nano fiber hemostatic material are 52.0 ± 2.2
×109A/L, there were significant differences between three, it will thus be seen that material surface of the present invention is porous, hydrophilic absorbency is high, has
Excellent platelet adhering function.
The present invention by the setting parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed and polarization parameter,
And then the microstructure and polarization performance of gelatin fiber film are controlled, gelatine nano fiber hemostatic material porous surface of the invention,
Hydrophilic absorbency is high, so that the present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, unit of the invention
The haemostatic effect of the gelatine nano fiber hemostatic material of volume is good, and the infection probability of wound location is small, and the present invention has good
Biocompatibility and biodegradability, are a kind of Novel absorbable hemostatic materials, and the present invention stops preparing gelatine nano fiber
Without the use of any chemical reagents in blood materials process, and simple production process, the present invention are a kind of preparation sides of Environmental Safety
Method.
Embodiment 6:
The preparation method that can promote the gelatine nano fiber hemostatic material of platelet aggregation, includes the following steps:
Step S1: it weighs 25g sheep bone gelatin and is added in 175g deionized water, be swollen 20min at normal temperature, to make bright
Xanthan molecule chain is opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 60 DEG C is dissolved into 30min, obtains dissolved gelatin
Solution, so that the unordered helical form of gelatin chains crimps, hydrophilic radical is controllably unfolded on strand；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, then carried out using spiral electrostatic spinning mode quiet
Electrospun, to obtain the gelatin solution after spinning, the parameter of the spinning solution storage device is that temperature is between 45-60 DEG C,
Voltage is 30kv, and electric current 0.16mA, spinning distance is 15cm, and the indoor environment temperature is the lace curtaining between 40-45 DEG C
Revolving speed 12r/min；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
Polarization parameter is polarised and is arranged under the action of piezoelectric field E2, and the mode of polarization process is Filamentous corona polarizing, described
Polarization parameter is voltage 55kv, electric current 2.6mA, and polarization distance is 10cm；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches 0.1-10mm；
Step S6: gelatin fiber film is removed, and in 40 DEG C of vacuum ovens after drying for 24 hours, is sealed with sterilizing bag, epoxy
Ethane sterilizing is placed on 4 DEG C of preservations, to obtain gelatine nano fiber hemostatic material.
As shown in Figure 1, detecting spinning fibre diameter by instrument is in normal distribution, main distribution is 300-870nm.
Gelatine nano fiber hemostatic material 0.10g of the invention, existing market control sample 0.10g and blank sample are taken, according to
Pooled plasma 5.0mL is added in the ratio of 0.02g/mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates
2h, with the platelet concentration in Full automatic animal cellanalyzer measurement blood.Measurement result are as follows: blank group 199.3 ± 2.9
×109A/L, market samples control group are 159.3 ± 14.4 × 109A/L, gelatine nano fiber hemostatic material are 51.1 ± 2.1
×109A/L, there were significant differences between three.It will thus be seen that material surface of the present invention is porous, hydrophilic absorbency is high, has
Excellent platelet adhering function.
The present invention by the setting parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed and polarization parameter,
And then the microstructure and polarization performance of gelatin fiber film are controlled, gelatine nano fiber hemostatic material porous surface of the invention,
Hydrophilic absorbency is high, so that the present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, unit of the invention
The haemostatic effect of the gelatine nano fiber hemostatic material of volume is good, and the infection probability of wound location is small, and the present invention has good
Biocompatibility and biodegradability, are a kind of Novel absorbable hemostatic materials, and the present invention stops preparing gelatine nano fiber
Without the use of any chemical reagents in blood materials process, and simple production process, the present invention are a kind of preparation sides of Environmental Safety
Method.
Embodiment 7:
The preparation method that can promote the gelatine nano fiber hemostatic material of platelet aggregation, includes the following steps:
Step S1: it weighs 20g ox bone gelatin and is added in 175g deionized water, be swollen 20min at normal temperature, to make bright
Xanthan molecule chain is opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 60 DEG C is dissolved into 30min, obtains dissolved gelatin
Solution, so that the unordered helical form of gelatin chains crimps, hydrophilic radical is controllably unfolded on strand；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, then using multiple wire electrostatic spinning mode into
Row electrostatic spinning, to obtain the gelatin solution after spinning, it is 40-45 DEG C that the parameter of the spinning solution storage device, which is temperature,
Voltage is 60kv, and electric current 0.12mA, spinning distance is 17cm, and the indoor environment temperature is 35-36 DEG C, and the lace curtaining turns
Fast 10r/min；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
Polarization parameter is polarised and is arranged under the action of piezoelectric field E2, and the mode of polarization process is thermal poling, the polarization ginseng
Number is voltage 40kv, electric current 1.9mA, and polarization distance is 8cm；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches 0.1-10mm；
Step S6: gelatin fiber film is removed, and in 40 DEG C of vacuum ovens after drying for 24 hours, is sealed with sterilizing bag, epoxy
Ethane sterilizing is placed on 4 DEG C of preservations, to obtain gelatine nano fiber hemostatic material.
As shown in Figure 1, detecting spinning fibre diameter by instrument is in normal distribution, main distribution is 300-
1200nm。
Gelatine nano fiber hemostatic material 0.05g of the invention, existing market control sample 0.05g and blank sample are taken, according to
Pooled plasma 5.0mL is added in the ratio of 0.01g/mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates
2h, with the platelet concentration in Full automatic animal cellanalyzer measurement blood.Measurement result are as follows: blank group 199.3 ± 2.9
×109A/L, market samples control group are 174.3 ± 10.0 × 109A/L, gelatine nano fiber hemostatic material be 135.0 ±
1.4×109A/L, there were significant differences between three.It will thus be seen that material surface of the present invention is porous, hydrophilic absorbency is high, tool
There is excellent platelet adhering function.
The present invention by the setting parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed and polarization parameter,
And then the microstructure and polarization performance of gelatin fiber film are controlled, gelatine nano fiber hemostatic material porous surface of the invention,
Hydrophilic absorbency is high, so that the present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, unit of the invention
The haemostatic effect of the gelatine nano fiber hemostatic material of volume is good, and the infection probability of wound location is small, and the present invention has good
Biocompatibility and biodegradability, are a kind of Novel absorbable hemostatic materials, and the present invention stops preparing gelatine nano fiber
Without the use of any chemical reagents in blood materials process, and simple production process, the present invention are a kind of preparation sides of Environmental Safety
Method.
Embodiment 8:
The preparation method that can promote the gelatine nano fiber hemostatic material of platelet aggregation, includes the following steps:
Step S1: it weighs 20g ox bone gelatin and is added in 175g deionized water, be swollen 20min at normal temperature, to make bright
Xanthan molecule chain is opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 60 DEG C is dissolved into 30min, obtains dissolved gelatin
Solution, so that the unordered helical form of gelatin chains crimps, hydrophilic radical is controllably unfolded on strand；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, is then set
Set the parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed, then using multiple wire electrostatic spinning mode into
Row electrostatic spinning, to obtain the gelatin solution after spinning, it is 40-45 DEG C that the parameter of the spinning solution storage device, which is temperature,
Voltage is 60kv, and electric current 0.12mA, spinning distance is 17cm, and the indoor environment temperature is 35-36 DEG C, and the lace curtaining turns
Fast 10r/min；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to height again
Polarization parameter is polarised and is arranged under the action of piezoelectric field E2, and the mode of polarization process is Filamentous corona polarizing, described
Polarization parameter is voltage 80kv, electric current 4.5mA, and polarization distance is 12cm；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches 0.1-10mm；
Step S6: gelatin fiber film is removed, and in 40 DEG C of vacuum ovens after drying for 24 hours, is sealed with sterilizing bag, epoxy
Ethane sterilizing is placed on 4 DEG C of preservations, to obtain gelatine nano fiber hemostatic material.
As shown in Figure 1, detecting spinning fibre diameter by instrument is in normal distribution, main distribution is 300-
1200nm。
Gelatine nano fiber hemostatic material 0.05g of the invention, existing market control sample 0.05g and blank sample are taken, according to
Pooled plasma 5.0mL is added in the ratio of 0.01g/mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates
2h, with the platelet concentration in Full automatic animal cellanalyzer measurement blood.Measurement result are as follows: blank group 199.3 ± 2.9
×109A/L, market samples control group are 174.3 ± 10.0 × 109A/L, gelatine nano fiber hemostatic material be 130.2 ±
1.0×109A/L, there were significant differences between three.It will thus be seen that material surface of the present invention is porous, hydrophilic absorbency is high, tool
There is excellent platelet adhering function.
The present invention by the setting parameter of spinning solution storage device, indoor environment temperature, lace curtaining revolving speed and polarization parameter,
And then the microstructure and polarization performance of gelatin fiber film are controlled, gelatine nano fiber hemostatic material porous surface of the invention,
Hydrophilic absorbency is high, so that the present invention has the advantages that hydrophilic absorbency is good, platelet adhesion rate is high, unit of the invention
The haemostatic effect of the gelatine nano fiber hemostatic material of volume is good, and the infection probability of wound location is small, and the present invention has good
Biocompatibility and biodegradability, are a kind of Novel absorbable hemostatic materials, and the present invention stops preparing gelatine nano fiber
Without the use of any chemical reagents in blood materials process, and simple production process, the present invention are a kind of preparation sides of Environmental Safety
Method.
It should be noted that listed above is only a kind of specific embodiment of the invention.It is clear that the invention is not restricted to
Upper embodiment, can also be there are many deforming, in short, those skilled in the art can directly lead from present disclosure
Out or all deformations for associating, it is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method for the gelatine nano fiber hemostatic material that can promote platelet aggregation, which is characterized in that including such as
Lower step:
Step S1: gelatin being placed in solvent and is dissolved, and obtains gelatin solution, is then placed in gelatin solution in container often
Temperature swelling 20-40min, so that gelatin chains are opened, the gelatin solution being swollen；
Step S2: the stirred in water bath that the gelatin solution of swelling is put in 40-70 DEG C is dissolved into 10-60min, is obtained dissolved bright
Sol solution；
Step S3: dissolved gelatin solution is poured into spinning solution storage device, is then turned on reception lace curtaining, and then setting is spun
The silk parameter of liquid storage device, indoor environment temperature, lace curtaining revolving speed, electrostatic spinning is then carried out, thus after obtaining spinning
Gelatin solution；
Step S4: the gelatin solution after spinning is deposited under the action of high voltage electric field E1, is then transported to high-voltage electricity again
It is polarised under the action of the E2 of field and polarization parameter is set；
Step S5: being iteratively repeated step S4, until gelatin fiber film reaches certain thickness；
Step S6: gelatin fiber film being removed, is then dried in a vacuum drying oven, is then packed with sterilizing bag, is then sterilized,
Then in shady and cool kept dry, to obtain gelatine nano fiber hemostatic material.
2. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is that the certain thickness is between 0.1-10mm.
3. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is that for the mass percent of the gelatin in institute's gelatine solution between 5%-25%, solvent is deionized water or pure water
Or high purity water.
4. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is that the parameter of the spinning solution storage device is that temperature is between 40 DEG C -70 DEG C, and voltage is between 15kv-80kv, electric current
Between 0mA-0.5mA, spinning distance is between 5cm-30cm.
5. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is that the indoor environment temperature is between 30 DEG C -60 DEG C, between the revolving speed 1r/min-10r/min of the lace curtaining.
6. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is that the polarization parameter is between voltage 30kv-70kv, and for electric current between 1mA-10mA, polarization distance is 5cm-30cm
Between.
7. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is that the temperature dried in a vacuum drying oven is between 35 DEG C -80 DEG C, and the dry time is between 2h-48h.
8. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is that the method for the electrostatic spinning is spiral textile or needle spinning or metal silk spinning or sheet metal is spun or metal salient point is spun.
9. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is that the method for the polarization process is corona polarizing or thermal poling or soft X-ray polarizes or radiation-polarizing or magnetic polarization, described
The method of sterilizing is ethylene oxide sterilizing or ultraviolet sterilization or radiosterilization.
10. it can promote the preparation method of the gelatine nano fiber hemostatic material of platelet aggregation according to claim 1, it is special
Sign is, institute's gelatine solution is that edible gelatin solution is or medical gelatin solution or pharmagel solution.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110433325A (en) * | 2019-07-26 | 2019-11-12 | 杭州中科润德生物技术发展有限公司 | A kind of protide high polymer nanometer fiber hemostatic material and its preparation method and application |
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