CN110031460B - Kit for detecting senile dementia - Google Patents
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- CN110031460B CN110031460B CN201910391578.7A CN201910391578A CN110031460B CN 110031460 B CN110031460 B CN 110031460B CN 201910391578 A CN201910391578 A CN 201910391578A CN 110031460 B CN110031460 B CN 110031460B
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Abstract
The invention belongs to the field of biological medicine, and particularly relates to a kit for detecting senile dementia, which comprises a box body structure, two test tube placing structures, two movable structures and two inspection structures; the test tube placing barrel is used for placing a test tube filled with the serum of the patient to be tested; the test structure is used for adding reagent into the test tube of the serum of the patient to be tested and carrying out contrast test on the test tube of the serum of the patient to be tested. The kit for detecting senile dementia can simultaneously detect the content of superoxide dismutase and the content of malondialdehyde in the serum of a patient, and the content of superoxide dismutase and the content of malondialdehyde in the serum of the patient are determined by comparing the color displayed by adding a reagent into the contrast card and the detection serum, so that whether the patient suffers from senile dementia or not is judged, and the kit has the advantages of simple structure, simplicity and convenience in operation and high diagnosis speed.
Description
[ technical field ] A method for producing a semiconductor device
The invention belongs to the field of biological medicines, and particularly relates to a kit for detecting senile dementia.
[ background of the invention ]
Dementia is a syndrome of hypofunction of the higher nerves of the brain caused by various causes, and the hypofunction is generally chronically progressive. Dementia is a descriptive term for clinical syndromes and not a disease or pathological process. Dementia, which is manifested as a variety of higher cortical functional impairment, is an acquired, persistent intellectual disorder, i.e., in the case of unconscious disorders, there are at least 3 functional impairments, such as cognitive and memory dysfunction, in 5 psychological activities, such as cognitive, memory, speech, visuospatial skills, emotion or personality, and affecting social and life functions of the person. Dementia can be classified into 3 types, based on the common causes of its onset, senile dementia, vascular dementia, and other mixed dementias that affect brain function either fulminantly or secondarily. Among them, senile dementia, i.e., Alzheimer Disease (AD), is one of the main types of senile dementia, and is a degenerative Disease of the nervous system in which recent memory impairment, mental deterioration, personality, and behavior are progressively changed into main clinical symptoms. The disease mostly occurs in the old, the patients lose self-care ability and live through dependent life, and the clinical manifestations of the disease have various cortical advanced dysfunctions, including memory, thinking, orientation, understanding, calculating, learning ability, language and judgment disorders, which seriously affect the life quality of the old. With the aging population, China has crossed into the aging countries, the mortality rate and the morbidity of AD rise year by year, and in the world, AD becomes the 4 th fatal disease after cardiovascular and cerebrovascular diseases and malignant tumors, and is one of the most common serious mental health problems harming the old at present.
Early diagnosis is considered to be of critical importance according to the Alzheimer's Association and the United states of research. Since most lesions then progress slowly. Early diagnosis and early treatment can greatly delay the occurrence of pathological changes and maintain the normal living standard of patients. Early accurate diagnosis also helps to distinguish real Alzheimer's disease patients from other patients who are suspected of having Alzheimer's disease (e.g., depression, drug side effects, drugs of abuse, vitamin deficiency, dehydration, bladder infection or thyroid problems), avoiding unnecessary, possibly harmful treatment due to misdiagnosis.
Currently, the diagnosis of alzheimer's disease involves a number of tests including: medical history, mental state assessment, physical examination, neurological examination, neuropsychological assessment, brain nuclear magnetic resonance, laboratory tests; therefore, the current diagnosis of the senile dementia is not only high in cost, but also not suitable for early screening; there is a need to develop a method suitable for in vitro early screening of patients with early AD and normal persons.
[ summary of the invention ]
In view of the above problems, the present invention provides a kit for detecting senile dementia, which is a kit for detecting senile dementia
The technical problem to be solved by the invention is realized by adopting the following technical scheme: a kit for detecting senile dementia comprises a box body structure, two test tube placing structures, two movable structures and two inspection structures;
the box body structure comprises a box body, a rotating plate, a clapboard and a ribbed plate; the box body is of a cuboid hollow structure, and the partition board is accommodated in the box body and divides the internal space of the box body into two oppositely sealed cavities; the rotating plate is rotatably connected with the top surface of the box body; the ribbed plate is arranged on the rotating plate; one side surface of the box body is provided with two light transmission grooves which are respectively communicated with the two cavities.
The test tube placing structure comprises a test tube placing barrel, a fixed rod and a fixed ring; the fixed rod is arranged on the outer side surface of the box body and is positioned above the light transmission groove; the fixing ring is fixedly connected with the fixing rod; the test tube placing barrel is movably accommodated in the fixed ring; the test tube placing barrel is of a hollow cylindrical structure with an opening at the upper part, and the relative bottom of the test tube placing barrel is in a hollow semi-sphere shape; the test tube is placed and has been seted up on the bucket and has been observed the groove and pierce through the groove, and printing opacity groove, observation groove and pierce through the trench and be located the coplanar to it is located between printing opacity groove and the observation groove to pierce through the groove. The test tube placing barrel is used for placing a test tube filled with serum of a patient to be tested.
The movable structure comprises a connecting plate, an annular groove, a groove rod, a circular plate and a motor; the connecting plate is arranged on the test tube placing barrel and is positioned at the bottom of the penetrating groove; the annular groove is arranged on the connecting plate; one end of the groove rod is movably accommodated in the annular groove, and the other end of the groove rod is fixedly connected with one surface of the circular plate; the motor is accommodated in the cavity and is fixedly connected with the box body, and a motor shaft of the motor extends out of the light transmission groove and is fixedly connected with the circular plate; the circular plate is positioned between the groove rod and the motor; the motor shaft of the motor and the circular plate have the same central axis.
The inspection structure comprises a lamp, a reagent tube, a reagent vessel and a comparison card; the lamp is arranged in the cavity; the reagent tube is fixedly arranged on the rotating plate, one end of the reagent tube is positioned on one side of the test tube placing barrel, and the other end of the reagent tube is positioned on one side of the rib plate; the reagent vessel is fixedly connected with one end of the reagent tube positioned on one side of the ribbed plate; the reagent dish is used for storing reagents; the reagent tube is used for dripping the reagent in the reagent dish into the corresponding test tube in the test tube placing barrel; the contrast card is arranged on the box body and is positioned between the two test tube placing barrels;
wherein the wavelength of the light emitted by one lamp is 420nm, and the wavelength of the light emitted by the other lamp is 530 nm;
one reagent vessel is filled with pyrogallol, and the other reagent vessel is filled with thiobarbituric acid;
the lamp with the light-emitting wavelength of 420nm, the reagent vessel filled with pyrogallol and the test tube placing barrel are positioned on the same plane;
a lamp with the light-emitting wavelength of 530nm, a reagent vessel filled with thiobarbituric acid and a test tube placing barrel in the reagent vessel are positioned on the same plane;
one of the comparison cards is used for marking the corresponding colors of the superoxide dismutase with different contents in the pyrogallol after being irradiated by a lamp with the light-emitting wavelength of 420 nm;
one of the comparison cards is used for marking the corresponding colors of different contents of malondialdehyde in thiobarbituric acid after being irradiated by a lamp with the light-emitting wavelength of 530 nm.
The reagent vessel is made of one of silica gel, rubber or PVC materials.
Due to the adoption of the technical scheme, the invention has the following beneficial effects: the kit for detecting senile dementia can simultaneously detect the content of superoxide dismutase and the content of malondialdehyde in the serum of a patient, and the content of superoxide dismutase and the content of malondialdehyde in the serum of the patient are determined by comparing the color displayed by adding a reagent into the contrast card and the detection serum, so that whether the patient suffers from senile dementia or not is judged, and the kit has the advantages of simple structure, simplicity and convenience in operation and high diagnosis speed.
[ description of the drawings ]
FIG. 1 is a schematic structural diagram of a kit for detecting Alzheimer's disease according to the present invention.
FIG. 2 is a schematic structural diagram of the kit for detecting Alzheimer's disease of the present invention.
FIG. 3 is a schematic view of a partial structure of the kit for detecting Alzheimer's disease of the present invention.
FIG. 4 is a schematic structural diagram of a test tube placement structure and a movable structure of the kit for detecting Alzheimer's disease of the present invention.
Description of the main elements
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[ detailed description ] embodiments
The following examples may help one skilled in the art to more fully understand the present invention, but are not intended to limit the invention in any way.
Referring to fig. 1-4, a kit for detecting senile dementia includes a box structure 1, two test tube placement structures 2, two movable structures 3, and two inspection structures 4;
the box body structure 1 comprises a box body 11, a rotating plate 12, a clapboard 13 and a ribbed plate 14; the box body 11 is of a cuboid hollow structure, and the partition plate 13 is accommodated in the box body 11 and divides the internal space of the box body 11 into two oppositely sealed cavities 15; the rotating plate 12 is rotatably connected with the top surface of the box body 11; in the present embodiment, the rotating plate 12 is hinged to the top surface of the box 11; the rib plate 14 is arranged on the rotating plate 12; two light transmission grooves 16 are arranged on one side surface of the box body 11, and the two light transmission grooves 16 are respectively communicated with the two cavities 15.
The test tube placing structure 2 comprises a test tube placing barrel 21, a fixed rod 22 and a fixed ring 23; the fixing rod 22 is arranged on the outer side surface of the box body 11 and is positioned above the light transmission groove 16; the fixing ring 23 is fixedly connected with the fixing rod 22; the test tube placing barrel 21 is movably accommodated in the fixing ring 23; the test tube placing barrel 21 is of a hollow cylinder structure with an opening at the upper part, and the test tube placing barrel 21 is of a hollow semi-sphere shape relative to the bottom; the test tube placing barrel 21 is provided with an observation groove 25 and a penetrating groove 26, the light transmitting groove 16, the observation groove 25 and the penetrating groove 26 are positioned on the same plane, and the penetrating groove 26 is positioned between the light transmitting groove 16 and the observation groove 25. The tube holding bucket 21 is used for holding a tube containing the serum of a patient to be tested.
The movable structure 3 comprises a connecting plate 31, an annular groove 32, a groove rod 33, a circular plate 34 and a motor 35; the connection plate 31 is mounted on the test tube placing barrel 21 and is positioned at the bottom of the penetration groove 26; the annular groove 32 is arranged on the connecting plate 31; one end of the groove rod 33 is movably accommodated in the annular groove 32, and the other end is fixedly connected with one surface of the circular plate 34; the motor 35 is accommodated in the cavity 15 and fixedly connected with the box body 11, and a motor shaft of the motor 35 extends out of the light-transmitting groove 16 and is fixedly connected with the circular plate 34; the circular plate 34 is positioned between the grooved rod 33 and the motor 35; the motor shaft of the motor 35 has the same central axis as the circular plate 34.
The test structure 4 is used for adding a reagent into a test tube of the serum of the patient to be tested and carrying out contrast test on the test tube of the serum of the patient to be tested; the inspection structure 4 comprises a lamp 41, a reagent tube 42, a reagent dish 43 and a comparison card 44; in the present embodiment, the reagent vessel 43 has a hollow spherical structure; the lamp 41 is mounted in the cavity 15; the reagent tube 42 is fixedly arranged on the rotating plate 12, one end of the reagent tube 42 is positioned at one side of the test tube placing barrel 21, and the other end is positioned at one side of the ribbed plate 14; the reagent vessel 43 is fixedly connected with one end of the reagent tube 42 positioned at one side of the rib plate 14; the reagent dish 43 is used for storing reagents; the reagent tube 42 is used for dropping the reagent in the reagent dish 43 into the corresponding test tube in the test tube placing barrel 21; the comparison card 44 is arranged on the box body 11, and the comparison card 44 is positioned between the two test tube placing barrels 21;
one of the lamps 41 emits light with a wavelength of 420nm, and the other lamp 41 emits light with a wavelength of 530 nm;
one of the reagent dishes 43 is filled with pyrogallol, and the other reagent dish 43 is filled with thiobarbituric acid;
the lamp 41 with the light-emitting wavelength of 420nm, the reagent vessel 43 filled with pyrogallol and one test tube placing barrel 21 are positioned on the same plane;
a lamp 41 with the light-emitting wavelength of 530nm is positioned on the same plane with the reagent vessel 43 filled with thiobarbituric acid and one of the test tube placing barrels 21;
one of the comparison cards 44 is used for marking the corresponding colors of the superoxide dismutase with different contents in the pyrogallol after being irradiated by a lamp 41 with the light-emitting wavelength of 420 nm;
one of the comparison cards 44 is used to mark the corresponding colors of different amounts of malondialdehyde in thiobarbituric acid after irradiation by a lamp 41 with a luminescence wavelength of 530 nm.
The rib plate 14 in this embodiment is a regular triangle plate-shaped structure, which is used to lean the rotating plate 12 on the box body 11, and prevent the reagent from leaking due to the extrusion of the box body 11 to the reagent dish 43;
in this embodiment, the reagent container 43 is made of an elastic material, such as silica gel, rubber or PVC material, so that the reagent in the reagent container 43 can be poured out from the reagent container 43.
When the kit for detecting the senile dementia is used, the serum of a patient to be detected is divided into two parts and put into test tubes; placing the two test tubes in two test tube placing buckets 21 respectively; rotating the rotating plate 12 to insert the reagent tubes 42 fixed to the rotating plate 12 into the test tubes, respectively; the reagents stored in the two reagent vessels 43 are respectively dripped into the test tubes; the starting motor 35 rotates to drive the circular plate 34 to rotate, the circular plate 34 rotates to drive the groove rod 33 to move relatively in the annular groove 32, and then the test tube placing barrel 21 is driven to move up and down relative to the fixing ring 23, so that the reaction of serum in the test tube and a reagent is accelerated; simultaneously turning on the lamp 41; the light emitted by the lamp 41 passes through the light-transmitting groove 16, the penetrating groove 26, the test tube and the observation groove 25 in sequence, the light finally penetrating through the test tube shows color in the observation groove 25, the color shown in the observation groove 25 is respectively compared with the color on the corresponding comparison card 44, the color shown in the test tube added with pyrogallol is compared with the comparison card 44 for marking the corresponding color of the superoxide dismutase with different content in the pyrogallol after the irradiation of the lamp 41 with the light-emitting wavelength of 420nm, and the content of the superoxide dismutase in the serum is obtained; comparing the color displayed by the test tube of the thiobarbituric acid with a comparison card 44 for marking the corresponding color of the malondialdehyde with different contents in the thiobarbituric acid after being irradiated by a lamp 41 with the light-emitting wavelength of 530nm, and obtaining the content of the malondialdehyde in the serum.
The principle is as follows: the pyrogallol is autoxidized into the tangeritol in the serum, and after the pyrogallol is irradiated by a lamp 41 with the luminous wavelength of 420nm, the content of the superoxide dismutase in the serum can be reflected according to the color change of the pyrogallol in the serum, and the content of the superoxide dismutase in the serum in a test tube can be obtained by comparing the color of the serum to be detected with the color of a measured comparison card 44.
The thiobarbituric acid reacts with malondialdehyde in serum to generate a red rice dumpling color product, the malondialdehyde content in the serum is reflected according to the color change of the thiobarbituric acid in the serum, and the color of the serum to be detected is compared with the color of the measured contrast card 44 to obtain the malondialdehyde content in the serum in the test tube;
malonaldehyde and superoxide dismutase are important parameter indexes for detecting senile dementia, and the content of malonaldehyde and superoxide dismutase is measured to carry out primary detection on the senile dementia of a patient;
in the present test example, three colors of light orange, orange and tangerine are marked on one of the comparison cards 44 from light to dark, and the content of malondialdehyde in the serum corresponding to the light orange is 3.0-4.5nmol/ml under the irradiation of the lamp 41 with the light-emitting wavelength of 420 nm; the content of malondialdehyde in blood serum corresponding to orange color is 4.6-5.5 nmol/ml; the content of malondialdehyde in the serum corresponding to the color of red orange is 6-10 nmol/ml; the content of the ultra-malondialdehyde in the serum of normal people is 3.0-5.5nmol/ml, and the content of the malondialdehyde in the serum of the senile dementia patient is far higher than the content, so when the pyrogallol is added into the serum and then the serum is irradiated by a lamp 41 with the light-emitting wavelength of 420nm to be red-orange, the patient can be judged to be the senile dementia patient
The other contrast card 44 is marked with three colors of light red, brown and red zongzi from light to dark; under the irradiation of a lamp with the light-emitting wavelength of 530nm, the content of superoxide dismutase in the corresponding serum with light red is 20-55 NU/ml; brown corresponds to a superoxide dismutase content of 60-99NU/ml in the serum; the content of superoxide dismutase in the blood serum corresponding to the color of the red rice dumplings is 100-; the superoxide dismutase content in the serum of normal human is 60-100NU/ml, and the superoxide dismutase content in the serum of the senile dementia patient is far lower than the superoxide dismutase content, so that the patient can be judged as the senile dementia patient when the serum is irradiated by a lamp 41 with the light-emitting wavelength of 530nm to be light red after the thiobarbituric acid is added into the serum.
When the superoxide dismutase in the serum detected by the patient is light red and the malondialdehyde is orange, the patient can be judged to have the senile dementia.
When the superoxide dismutase in the serum detected by the patient is brown or red rice dumpling color and the malondialdehyde is light orange or orange color, the patient can be judged not to suffer from the senile dementia.
The kit for detecting the senile dementia can simultaneously detect the content of the superoxide dismutase and the content of the malondialdehyde in the serum of a patient, and the content of the superoxide dismutase and the content of the malondialdehyde in the serum of the patient are determined by comparing the color displayed by adding a reagent into the contrast card and the detection serum, so that whether the patient suffers from the senile dementia or not is judged.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. A kit for detecting senile dementia is characterized by comprising a box body structure, two test tube placing structures, two movable structures and two inspection structures;
the box body structure comprises a box body, a rotating plate, a clapboard and a ribbed plate; the box body is of a cuboid hollow structure, and the partition board is accommodated in the box body and divides the internal space of the box body into two oppositely sealed cavities; the rotating plate is rotatably connected with the top surface of the box body; the ribbed plate is arranged on the rotating plate; one side surface of the box body is provided with two light transmission grooves which are respectively communicated with the two cavities;
the test tube placing structure comprises a test tube placing barrel, a fixed rod and a fixed ring; the fixed rod is arranged on the outer side surface of the box body and is positioned above the light transmission groove; the fixing ring is fixedly connected with the fixing rod; the test tube placing barrel is movably accommodated in the fixed ring; the test tube placing barrel is provided with an observation groove and a penetration groove, the light transmission groove, the observation groove and the penetration groove are positioned on the same plane, and the penetration groove is positioned between the light transmission groove and the observation groove; the test tube placing barrel is used for placing a test tube filled with the serum of the patient to be tested;
the movable structure is used for enabling the test tube placing barrel to move up and down relative to the fixing ring and comprises a connecting plate, an annular groove, a groove rod, a circular plate and a motor; the connecting plate is arranged on the test tube placing barrel and is positioned at the bottom of the penetrating groove; the annular groove is arranged on the connecting plate; one end of the groove rod is movably accommodated in the annular groove, and the other end of the groove rod is fixedly connected with one surface of the circular plate; the motor is accommodated in the cavity and is fixedly connected with the box body, and a motor shaft of the motor extends out of the light transmission groove and is fixedly connected with the circular plate; the circular plate is positioned between the groove rod and the motor;
the test structure is used for adding a reagent into a test tube of the serum of the patient to be tested and carrying out contrast test on the test tube of the serum of the patient to be tested; the inspection structure comprises a lamp, a reagent tube, a reagent vessel and a comparison card; the lamp is arranged in the cavity; the reagent tube is fixedly arranged on the rotating plate, one end of the reagent tube is positioned on one side of the test tube placing barrel, and the other end of the reagent tube is positioned on one side of the rib plate; the reagent vessel is fixedly connected with one end of the reagent tube positioned on one side of the ribbed plate; the reagent dish is used for storing reagents; the reagent tube is used for dripping the reagent in the reagent dish into the corresponding test tube in the test tube placing barrel; the contrast card is arranged on the box body and is positioned between the two test tube placing barrels;
wherein the wavelength of the light emitted by one lamp is 420nm, and the wavelength of the light emitted by the other lamp is 530 nm;
wherein the reagent contained in one reagent vessel is pyrogallol, and the reagent contained in the other reagent vessel is thiobarbituric acid;
the lamp with the light-emitting wavelength of 420nm, the reagent vessel filled with pyrogallol and the test tube placing barrel are positioned on the same plane;
a lamp with the light-emitting wavelength of 530nm, a reagent vessel filled with thiobarbituric acid and a test tube placing barrel in the reagent vessel are positioned on the same plane;
one of the comparison cards is used for marking the corresponding colors of the superoxide dismutase with different contents in the pyrogallol after being irradiated by a lamp with the light-emitting wavelength of 420 nm;
one of the comparison cards is used for marking the corresponding colors of different contents of malondialdehyde in thiobarbituric acid after being irradiated by a lamp with the light-emitting wavelength of 530 nm.
2. The kit for detecting senile dementia according to claim 1, wherein a contrast card is marked with three colors of light orange, orange and tangerine from light to dark, the light orange corresponds to the content of malondialdehyde in serum of 3.0-4.5 nmol/ml; the content of malondialdehyde in blood serum corresponding to orange color is 4.6-5.5 nmol/ml; the content of malondialdehyde in the serum corresponding to the color of red orange is 6-10 nmol/ml;
the other contrast card is marked with three colors of light red, brown and red rice dumpling color from light to dark; the light red color corresponds to the superoxide dismutase content in the serum of 20-55 NU/ml; brown corresponds to a superoxide dismutase content of 60-99NU/ml in the serum; the color of the red rice dumpling corresponds to the content of superoxide dismutase in serum of 100-.
3. The kit for detecting senile dementia according to claim 1, wherein the rib has a regular triangular plate-like structure.
4. The kit for detecting senile dementia according to claim 1, wherein the reagent vessel is made of silica gel, rubber or PVC material.
5. The kit for detecting senile dementia according to claim 1, wherein the rotation plate is hinged to the top surface of the case body.
6. The kit for detecting senile dementia according to claim 1, wherein the motor shaft of the motor has the same central axis as the circular plate.
7. The kit for detecting senile dementia according to claim 1, wherein the reagent vessel has a hollow spherical structure.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910391578.7A CN110031460B (en) | 2019-05-10 | 2019-05-10 | Kit for detecting senile dementia |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910391578.7A CN110031460B (en) | 2019-05-10 | 2019-05-10 | Kit for detecting senile dementia |
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| CN110031460B true CN110031460B (en) | 2021-07-20 |
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Citations (4)
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| CN1871338A (en) * | 2004-11-30 | 2006-11-29 | 株式会社Dml | Measuring kit for microbe in liquid sample, and relevant measuring method and measuring apparatus |
| CN204536207U (en) * | 2015-03-09 | 2015-08-05 | 陈异子 | A kind of Portable civil food safety detection device based on reagent mode |
| CN107238711A (en) * | 2017-05-18 | 2017-10-10 | 无锡市精神卫生中心 | A kind of diagnostic kit and its detection method for detecting Alzheimer disease peripheral blood protein marker |
| CN206563714U (en) * | 2016-11-01 | 2017-10-17 | 上海碧云天生物技术有限公司 | A kind of glutathione reductase detection kit |
-
2019
- 2019-05-10 CN CN201910391578.7A patent/CN110031460B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1871338A (en) * | 2004-11-30 | 2006-11-29 | 株式会社Dml | Measuring kit for microbe in liquid sample, and relevant measuring method and measuring apparatus |
| CN204536207U (en) * | 2015-03-09 | 2015-08-05 | 陈异子 | A kind of Portable civil food safety detection device based on reagent mode |
| CN206563714U (en) * | 2016-11-01 | 2017-10-17 | 上海碧云天生物技术有限公司 | A kind of glutathione reductase detection kit |
| CN107238711A (en) * | 2017-05-18 | 2017-10-10 | 无锡市精神卫生中心 | A kind of diagnostic kit and its detection method for detecting Alzheimer disease peripheral blood protein marker |
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| CN110031460A (en) | 2019-07-19 |
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