CN110029100A - A kind of nano combined strain occlusion vehicle - Google Patents
A kind of nano combined strain occlusion vehicle Download PDFInfo
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- CN110029100A CN110029100A CN201910183009.3A CN201910183009A CN110029100A CN 110029100 A CN110029100 A CN 110029100A CN 201910183009 A CN201910183009 A CN 201910183009A CN 110029100 A CN110029100 A CN 110029100A
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Abstract
The present invention provides a kind of nano combined strain occlusion vehicle, specifically, the nano combined strain occlusion vehicle includes immobilization nanosphere and composite bacteria preparation, nano combined strain occlusion vehicle provided by the invention is due to multi-pore structure, therefore there is very strong mass transfer ability, it is applicable not only to sewage treatment field, it can also be used to the fields such as biofermentation, it is particularly suitable for the field for needing to immobilize the bacterium in microenvironment, embedding rate is high, viable count is high, and occlusion vehicle is prepared in balling-up effect, mechanical strength and mass transfer performances etc. general performance are very excellent, service life is up to 18 months.
Description
Technical field
The present invention relates to occlusion vehicle field, a kind of particularly nano combined strain occlusion vehicle.
Background technique
Immobilized microorganism technique is to fix special microorganism on the carrier of certain medium, makes its highly dense and protects
Bioactivity is held, the biotechnology that can quickly, be largely proliferated under optimum conditions.This technology is applied to wastewater treatment, has
Conducive to the concentration for improving microorganism (the especially microorganism of specific function) in bioreactor, it is unfavorable to be conducive to microorganism resistance
The influence of environment, the separation of solid and liquid after being conducive to reaction, time needed for shortening processing.
Immobilized microorganism technique is the new technique to grow up since the sixties.Immobilized microorganism technique refers to
With either physically or chemically limiting by free microorganism cell, animal and plant cells, organelle or enzyme or be located in a certain specific sky
Between in range, retain its intrinsic catalytic activity, and can be repeated and be used continuously technology.Had using immobilized microorganism technique
Following advantages: being conducive to improve microorganism concn and purity in bioreactor, and time needed for shortening reaction, reduction processing is set
The construction investment and cost applied；Be conducive to the separation of solid and liquid of reactor, react easily controllable, sludge yield is few；Be conducive to remove
Nitrogen and removing high-enriched organics or other poisonous and harmful substances difficult to degrade, can exempt the secondary pollution etc. of Treatment of Sludge.
Due to its unique advantage in terms of wastewater treatment, rapid development is obtained in the nearly more than ten years.It is by original
Single immobilised enzymes, that fixation of microbial cell develops to immobilization animal and plant cells, immobilized cell device, immobilization is primary
Plastid, immobilized microorganism conidium and enzyme are fixed with microbial cell, aerobic microbiological with combining for anaerobe.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of nano combined strain occlusion vehicles.
The present invention is realized with following technical solution:
A kind of nano combined strain occlusion vehicle, the nano combined strain occlusion vehicle include immobilization nanosphere and
Composite bacteria preparation.
Further, the immobilization nanosphere includes % head product one, head product two and head product three.
Further, the head product one the preparation method comprises the following steps: by 100mg guar hydroxypropyltrimonium ammonium chloride 4
It is dissolved in 50ml dimethyl formamide solution at DEG C and obtains solution one, PEG and HATU is added into dimethyl formamide solution, with
The ratio of 48mg HATU/2mL dimethylformamide carries out polypeptide condensation reaction, molten to 100mL dimethylformamide while stirring
146mg PEG is added in liquid, 10 minutes acquisition solution two is reacted in darkroom, reacts with the ratio mixed solution one of 1:19 and solution two
20min obtains solution three, and 49 μ L triethylamines are added into solution three and are stirred overnight acquisition solution four in darkroom, next day is true
Sky removal DMF, surplus materials is dissolved in 100mL trifluoroacetic acid, is protected from light stirring 3h and is obtained solution five, trifluoro is removed in vacuo in next day
Acetic acid obtains head product one, is lyophilized spare.
Further, the head product two the preparation method comprises the following steps: 600mg poly-gamma-glutamic acid is dissolved in 120mL DMSO
In, 176mg HATU is added, stirs 20min at room temperature and obtains solution six, it is 15.1%mg/mL's that concentration is added into solution six
PF-DMSO solution, stirring 10min obtain solution seven, and 95 μ L triethylamines are added into solution seven and are stirred overnight in darkroom
Solution eight is obtained, purifying obtains head product two.
Further, the head product three obtains the preparation method comprises the following steps: the PGA of 40mg acid form is dissolved in 5mL DMSO
The first mixed liquor is obtained, 17.6mg HATU is then added into the first mixed liquor, stirs 10 minutes, the tertiary fourth oxygen of 9.8 μ L is then added
Carbonyl-polyethylene glycol-amino obtains the second mixed liquor and 12 μ L triethylamines is added dropwise into the second mixed liquor, at room temperature after ten minutes
1 day acquisition third mixed liquor is stirred, simultaneously acquisition head product three is lyophilized in purified product.
Further, the immobilization nanosphere includes 4.8% head product one, 5.2% head product two, 3.8% primiparity
Object three.
Further, the composite bacteria preparation includes Pan Shi vibrios, oxidation ammonia Selective medium, aoxidizes microbacterium, is anti-
Nitrification Halomonas, sulphur oxidation win the good hot anaerobism dialister bacterium of this Salmonella, pyrolysis sugar, bacillus aneurinolytieus, mostly viscous class gemma
Bacillus, phosphorus ore comamonas, Arthrobacter uratoxydans, food epoxides replace bacillus rubidus, thermophilic carbon bacillus.
The process for fixation of the biological adsorption agent studied now mainly include the following types: absorption method, absorption method generally according to
Effect between organism and carrier, including Van der Waals force, hydrogen bond, electrostatic interaction, covalent bond and ionic bond, village between the two
Current potential plays an important role in the interaction of microbial body and carrier.It is the common active charcoal of absorption carrier, sawdust, porous
Glass, porous ceramics, magnetic iron ore, diatomite, silica gel, cellulose, polyurethane foams, ion exchange resin etc..It is a kind of
Simple and easy, mild condition process for fixation, but the organism fixed with it is not firm enough, is easy to fall off.
Cross-linking method, cross-linking method are also known as no-load immobilization method, are a kind of technique without carrier, pass through chemistry, physical means
Make the crosslinking that is attached to each other between biological cell.Chemical crosslink technique it be usually to utilize aldehydes, amine etc. that there are difunctional or more function
Covalent bond is formed between the crosslinking agent and organism of energy group to interconnect to form insoluble macromolecular and be fixed.Physics
Cross-linking method is referring to that in microbial cultivation process, the appropriate condition of culture for changing cell suspending liquid makes between microbial cell
Directly effect occurs and granulates or flocculates to realize immobilization, i.e., forms particle using the autoflocculation ability of microorganism itself
A kind of immobilization technology.
Investment, it is the most commonly used with investment in immobilization method of microorganism.Its principle is by biological cell
It is trapped in the network of gelatin polymer hole of water-insoluble, is formed by polymerization or by ion network, or pass through
Precipitation, or make cell retention by changing solvent, temperature, pH value.The network of gelatin polymer can prevent letting out for cell
Dew, while matrix can be allowed to penetrate into and diffused out with product.
Embedded material can be divided into two major classes: natural macromolecule amylose class, such as alginate, agar, gelatin, wherein with
At most, they have many advantages, such as that solidification is convenient, and immobilization density small to Ecotoxicology is high for sodium alginate and carragheen application,
But their Resistance to microbes performances are poor, mechanical strength is low, but crosslinking agent can be used to carry out stabilization processes, but vigor
It can decline again with mass-transfer performance.
Synthetic macromolecular compound, such as polypropylene phthalein amine, polyvinyl alcohol.The outstanding advantages of this kind of crosslinking agent are to resist micro- life
Object decomposability is good, high mechanical strength, stable chemical performance.But the formation condition of polymer network is more violent, to micro- life
The damage of object cell is larger, and the diversity and controllability that shape are bad.
The invention has the following advantages: nano combined strain occlusion vehicle provided by the invention is due to porous
Structure, therefore there is very strong mass transfer ability, it is applicable not only to sewage treatment field, additionally it is possible to the fields such as biofermentation are used for,
It is particularly suitable for the field for needing to immobilize the bacterium in microenvironment, embedding rate is high, and viable count reaches as high as 2.20 ×
108CFU/mL, and prepare occlusion vehicle balling-up effect, mechanical strength and in terms of general performance it is very excellent,
Service life is up to 18 months.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, the present invention will be made below further detailed
Description.
Embodiment 1: the preparation of occlusion vehicle head product
It is molten that 100mg guar hydroxypropyltrimonium ammonium chloride is dissolved in the acquisition of 50ml dimethyl formamide solution at 4 DEG C
PEG and HATU is added into dimethyl formamide solution for liquid one, is carried out with the ratio of 48mg HATU/2mL dimethylformamide
146mg PEG is added in polypeptide condensation reaction into 100mL dimethyl formamide solution while stirring, and darkroom, which is reacted 10 minutes, to be obtained
Obtain two (NH of solution2-PEG3- NH-Boc), 20 minutes acquisition solution three is reacted with the ratio mixed solution one of 1:19 and solution two,
49 μ L triethylamines are added into solution three and are stirred overnight acquisition solution four in darkroom, DMF is removed in vacuo in next day, will be remaining
Substance is dissolved in 100mL trifluoroacetic acid, is protected from light stirring 3h and is obtained solution five, and trifluoroacetic acid is removed in vacuo in next day, obtains head product
One (amino guar gum-PEG- amino polymer) is lyophilized spare.
600mg poly-gamma-glutamic acid (PGA) is dissolved in 120mL DMSO, 176mg HATU is added, stirs at room temperature
20min obtains solution six, and PF (phenolic resin)-DMSO solution that concentration is 15.1%mg/mL, stirring are added into solution six
10min obtains solution seven, and 95 μ L triethylamines are added into solution seven and are stirred overnight acquisition solution eight in darkroom, purifying obtains
It obtains head product two (polyglycolic acid-tertbutyloxycarbonyl-polyethylene glycol-amino).
The PGA of 40mg acid form is dissolved in 5mL DMSO and obtains the first mixed liquor, is then added into the first mixed liquor
Enter 17.6mg HATU, stir 10 minutes, 9.8 μ L tertbutyloxycarbonyl-polyethylene glycol-amino are then added and obtain the second mixed liquor,
After ten minutes, 12 μ L triethylamines are added dropwise into the second mixed liquor, stir 1 day acquisition third mixed liquor at room temperature, purified product is simultaneously
Freeze-drying obtains head product three.
Embodiment 2: the preparation of occlusion vehicle
The occlusion vehicle be immobilization nanosphere, specifically, the preparation method is as follows: head product one, head product two, just
The concentration of product three is excessive or very few will affect embeds effect and/or performance, to determine head product one, head product two, primiparity
The optimal proportion of object three, by head product one, head product two and head product three, by different proportion mixing, (as shown in table 1, each component is dense
Degree shows that surplus is solvent with percentage), heating water bath to 90 DEG C or so dissolutions is cooled to 40 DEG C or so and obtains complex colloid (1-
10, CK).5%Ca (NO is added dropwise in 50mL each component complex colloid respectively under oscillating condition3)21h is impregnated in solution to be received
Meter Wei Qiu saves the nanosphere of acquisition for 24 hours at -20 DEG C, finally thaw at RT obtain immobilization nanosphere, -4 DEG C
It stores spare.
1. complex colloid component ratio of table
Embodiment 4: the acquisition of high COD sewage removal composite bacteria
High COD sewage is derived from seawater circulation water cultivating system (Qingdao Zhongke Seawater Treatment Co., Ltd.), for 24 hours interior acquisition one
Grade is discharged 12 times and mixes to be detected.It uses high COD sewage as culture solution culture, goes 500ml triangular flask 10, every bottle of addition
Primary dcreening operation culture medium: tryptone 2g, yeast extract 1g, NaCl 25g, 90ml high COD sewage, at 28 DEG C, 100r/min revolving speed
Lower shaking table culture 7 days is transferred in secondary screening culture medium by 20% inoculum concentration and is continued after having a certain amount of thallus to generate in culture medium
Culture 7 days, secondary screening culture medium: tryptone 2g, yeast extract 1g, NaCl 25g, 190ml high COD sewage are every other day surveyed
Random sample product OD600 value and COD value choose the high triangular flask of COD removal rate, draw the culture in the high triangular flask of COD removal rate
Liquid dilutes by a certain percentage, is coated on LB solid medium, and the different single colonie of picking feature is inoculated into respectively after purification
On solid medium, the faster bacterial strain of the speed of growth is cultivated and chooses, alkaline lysis is small to be mentioned 16S rDNA and carry out 16S rDNA
Sequence analysis (sequence analysis is completed by Shanghai Major Biological Medical Technology Co., Ltd.), carries out BLAST comparison to sequencing result,
It obtains high COD sewage to go in degerming 22, Pseudomonas title and deposit number are as shown in table 2.
2 high COD sewage of table goes degerming
Embodiment 5: the acquisition of composite bacteria preparation
Effective strain combination is obtained by Comparison Study, composite bacteria preparation includes Pan Shi vibrios, oxidation ammonia vacation promise card
The good hot anaerobism dialister bacterium of this Salmonella, pyrolysis sugar, solution thiamine bud are won in Salmonella, oxidation microbacterium, denitrification Halomonas, sulphur oxidation
Spore bacillus, phosphorus ore comamonas, Arthrobacter uratoxydans, eats epoxides alternating bacillus rubidus, is thermophilic Paenibacillus polymyxa
Carbon bacillus.Above-mentioned strain combination best in quality number are as follows: 5.5 parts of Pan Shi vibrios, oxidation 2.5 parts of ammonia Selective medium, oxygen
1.5 parts of this Salmonella, the good hot anaerobism dialister bacterium 10.5 of pyrolysis sugar are won in 7.5 parts of microbacterium of change, 8 parts of denitrification Halomonas, sulphur oxidation
Part, 12.5 parts of bacillus aneurinolytieus, 3 parts of Paenibacillus polymyxa, 1.5 parts of phosphorus ore comamonas, uric acid aoxidize pole
0.8 part of bacterium, food epoxides replace 11.1 parts of bacillus rubidus, 7 parts of thermophilic carbon bacillus.
Embodiment 6: the assembling of nano combined strain occlusion vehicle
Head product one, head product two are separately added into water, is dissolved by heating, is obtained the first gel solution, make described first
Gel solution is cooling, is uniformly mixed composite bacteria preparation described in embodiment 5 to obtain the second gel solution with head product three, by the
One gel solution mixes with the second gel solution and 5%Ca (NO is added dropwise3)2Solution, it is above-mentioned first with PBS solution wash products
Product one, head product two, head product three obtain the nano combined strain embedding of heterogeneity by the proportion of different proportion described in table 1
Carrier.
The physicochemical property for measuring immobilization nanosphere measures the physics and chemistry of the immobilization nanosphere by test
Matter, specifically, expansion rate, strength factor including immobilization nanosphere, embedding rate, measuring method is as follows.
Test the expansion rate of immobilization nanosphere: test immobilization nanosphere diameter is D, and it is micro- to choose immobilization nanometer
Ball impregnates in deionized water for 24 hours, and a diameter of D12 is measured under microscope, expands rate coefficient=D12/D, measurement result such as table 3
It is shown.
The measurement of strength factor: immobilization nanosphere is put into 150mL conical flask, and 75mL deionized water is added, in
100r/min, 30 DEG C of constant temperature oscillations complete the ratio that immobilization nanosphere accounts for former immobilization nanosphere sum, measurement afterwards for 24 hours
The results are shown in Table 3.
Measure immobilization nanosphere embedding rate: immobilization nanosphere embedding rate is the viable bacteria in immobilization nanosphere
Number accounts for the percentage for embedding preceding viable count sum.Total plate count X before statistics embeds, the nano combined strain embedding for claiming made
Carrier gross mass G1, takes out 1g or so nano combined strain occlusion vehicle, and precise measurement its quality G2 after being fully ground, is counted
Total plate count Y after being embedded, embedding rate=Y/X, measurement result are as shown in table 3.
3. immobilization nanosphere performance test of table
To take into account two aspect factor for the treatment of effect and economy, immobilization nanosphere practical performance is strong, embedding rate highest,
And it is best in the general performance of balling-up effect, mechanical strength and mass transfer performances to prepare occlusion vehicle.To sum up, the nano combined bacterium
Kind occlusion vehicle includes 4.8% head product one, 5.2% head product two, 3.8% head product three and composite bacteria preparation, described multiple
Combined bacteria kind preparation include Pan Shi vibrios, oxidation ammonia Selective medium, oxidation microbacterium, denitrification Halomonas, sulphur oxidation win this
Salmonella, pyrolysis sugar good hot anaerobism dialister bacterium, bacillus aneurinolytieus, Paenibacillus polymyxa, phosphorus ore comamonas, uric acid
Aoxidize arthrobacterium, food epoxides replaces bacillus rubidus, thermophilic carbon bacillus.
Nano combined strain occlusion vehicle provided by the invention is due to having very strong mass transfer with multi-pore structure
Ability is applicable not only to sewage treatment field, additionally it is possible to for fields such as biofermentations, be particularly suitable for needing in microenvironment
The field that immobilizes of bacterium, embedding rate is high, and viable count reaches as high as 2.20 × 108CFU/mL, and prepare embedding and carry
Body is best in the general performance of balling-up effect, mechanical strength and mass transfer performances, and service life is up to 18 months.
Using high COD sewage as research object, under the conditions of 28 DEG C, pH 6.5, it is multiple to add 0.2% nanometer of the present invention
Combined bacteria kind occlusion vehicle investigates ammonia nitrogen concentration, and initial COD is 6554.8mg/L, ammonia nitrogen concentration 5026.7mg/L, reacts one week
Afterwards, COD is reduced to 3227.4mg/L, and ammonia nitrogen concentration is reduced to 3099.2mg/L.BOD is dropped, the ratio of reasonable BOD/TN is also needed
The ratio of total nitrogen in example, i.e. BOD and sewage.This ratio of good efficient process organic sewage should 10 or more preferably,
That is, light has BOD, without nitrogen source, and do not drop BOD's.This is because the growth and breeding of microbial bacterial agent, need C,
N, the main nutrient elements such as P and other microelements etc.；Only C element (generally representing BOD) and lack other elements,
Zoogloea is not become.
The above disclosure is only the preferred embodiments of the present invention, cannot limit the right model of the present invention with this certainly
It encloses, therefore equivalent changes made in accordance with the claims of the present invention, is still within the scope of the present invention.
Claims (7)
1. a kind of nano combined strain occlusion vehicle, which is characterized in that the nano combined strain occlusion vehicle includes immobilization
Nanosphere and composite bacteria preparation.
2. carrier according to claim 1, which is characterized in that the immobilization nanosphere includes head product one, head product
Two and head product three.
3. carrier according to claim 1, which is characterized in that the head product one the preparation method comprises the following steps: by 100mg guar gum
Hydroxypropyl-trimethyl ammonium chloride is dissolved in 50ml dimethyl formamide solution at 4 DEG C and obtains solution one, molten to dimethylformamide
PEG and HATU is added in liquid, polypeptide condensation reaction is carried out with the ratio of 48mg HATU/2mL dimethylformamide, while stirring
146mg PEG is added into 100mL dimethyl formamide solution, 10 minutes acquisition solution two is reacted in darkroom, with the ratio of 1:19
Mixed solution one and solution two react 20min and obtain solution three, and 49 μ L triethylamines are added into solution three and stirring mixes in darkroom
It closes and obtains solution four overnight, DMF is removed in vacuo in next day, and surplus materials is dissolved in 100mL trifluoroacetic acid, is protected from light stirring 3h and obtains
Trifluoroacetic acid is removed in vacuo in solution five, next day, obtains head product one, is lyophilized spare.
4. carrier according to claim 1, which is characterized in that the head product two the preparation method comprises the following steps: by the poly- γ-of 600mg
Glutamic acid is dissolved in 120mL DMSO, and 176mg HATU is added, and is stirred 20min at room temperature and is obtained solution six, adds into solution six
Enter the PF-DMSO solution that concentration is 15.1%mg/mL, stirring 10min obtains solution seven, 95 μ L triethylamines are added into solution seven
And it is stirred overnight acquisition solution eight in darkroom, purifying obtains head product two.
5. carrier according to claim 1, which is characterized in that the head product three the preparation method comprises the following steps: by 40mg acidity shape
The PGA of formula, which is dissolved in 5mL DMSO, obtains the first mixed liquor, and 17.6mg HATU, stirring 10 are then added into the first mixed liquor
Minute, 9.8 μ L tertbutyloxycarbonyl-polyethylene glycol-amino are then added and obtain the second mixed liquor, after ten minutes, to the second mixing
12 μ L triethylamines are added dropwise in liquid, stir 1 day acquisition third mixed liquor at room temperature, simultaneously acquisition head product three is lyophilized in purified product.
6. carrier according to claim 1, which is characterized in that the immobilization nanosphere include 4.8% head product one,
5.2% head product two, 3.8% head product three.
7. carrier according to claim 1, which is characterized in that the composite bacteria preparation includes Pan Shi vibrios, oxidation ammonia vacation
The good hot anaerobism dialister bacterium of this Salmonella, pyrolysis sugar, solution thiamines are won in Nocard's bacillus, oxidation microbacterium, denitrification Halomonas, sulphur oxidation
Plain bacillus, Paenibacillus polymyxa, phosphorus ore comamonas, Arthrobacter uratoxydans, the red bar of food epoxides alternating
Bacterium, thermophilic carbon bacillus.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111100858A (en) * | 2019-12-23 | 2020-05-05 | 山东科技大学 | Immobilized microbial agent, offshore spilled oil treatment device carrying microbial agent and offshore spilled oil treatment method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW495551B (en) * | 1998-10-13 | 2002-07-21 | You-Gang Yuan | Manufacture method and application of microorganism immobilized carrier |
-
2019
- 2019-03-12 CN CN201910183009.3A patent/CN110029100A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW495551B (en) * | 1998-10-13 | 2002-07-21 | You-Gang Yuan | Manufacture method and application of microorganism immobilized carrier |
Non-Patent Citations (3)
| Title |
|---|
| ISHWAR BAJAJ等: ""Poly (glutamic acid) – A emerging biopolymer of commercial interest"", 《BIORESOURCE TECHNOLOGY》 * |
| ZSUZSANNA CSIKÓS等: ""Crosslinked poly-γ-glutamic acid based nanosystem for drug delivery"", 《JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY》 * |
| 何铁林主编: "《水处理化学品手册》", 31 May 2000, 化学工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111100858A (en) * | 2019-12-23 | 2020-05-05 | 山东科技大学 | Immobilized microbial agent, offshore spilled oil treatment device carrying microbial agent and offshore spilled oil treatment method |
| CN111100858B (en) * | 2019-12-23 | 2023-08-15 | 山东科技大学 | Immobilized microbial agent, offshore spilled oil treatment device carrying immobilized microbial agent and offshore spilled oil treatment method |
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