CN110029077A - A kind of salt tolerant growth-promoting bacteria strain Y4 and its application - Google Patents
A kind of salt tolerant growth-promoting bacteria strain Y4 and its application Download PDFInfo
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Abstract
The present invention relates to a kind of salt tolerant growth-promoting bacteria strain Y4, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC NO.17707, the deposit date is on May 5th, 2019, the 16S rDNA nucleotide sequence of the salt tolerant growth-promoting bacteria strain Y4 was as shown in SEQ ID NO.1.Bacterial strain Y4 of the invention can significantly improve the salt tolerant growth-promoting ability of tomato under salt stress, tomato growth-promoting agent is made in the bacterial strain, it is then injected into tomato seedling rhizosphere soil, the plant type, plant height and stem thickness of tomato can be significantly increased, the dry-matter accumulation of tomato seedling is remarkably promoted, and the root long to tomato seedling, surface area, root volume, tip of a root number has facilitation, slows down salt damage phenomenon, to promote the growth of tomato seedling, the salt tolerance of tomato is helped to improve.
Description
Technical field
The present invention relates to a kind of salt tolerant growth-promoting bacteria strain Y4 and its applications, belong to microorganisms technical field.
Background technique
Tomato be in the world plantation amount be only second to potato most solanaceous vegetable crops and flesh fruit and Solanaceae weight
Want model plant (Salvalaggio etc., 2017；Zhang Tianpeng and Yang Xinghong, 2018), liked and welcome by masses deeply, at present generation
The tomato variety reported within the scope of boundary is more than 3000 kinds, and global tomato production is faced with biology and the sternness of abiotic stress is chosen
War.Salt marsh is the big master for inhibiting plant growth in agricultural and reducing grain yield to limit global staple food crop productivity
Want environment-stress (Kaushal etc., 2016；Li Lulu etc., 2019).It is warm to be mainly planted in the world to salt medium sensitivity for tomato
And arid area, and often salinity is relatively high for the soil in these areas.With using increasingly the lacking of freshwater resources, population
The unreasonable use with natural resources is sharply expanded, the high drought-hit area of salinity, semiarid zone and coastal area Soil development are utilized
(Tank&Saraf, 2018) for it is had in agricultural production.The fast development of recent domestic tomato industrialized agriculture so that kind
It is more prominent that eggplant faces the problem of all kinds of stress such as Secondary salinization, therefore tomato salt-tolerant and its study on regulation just seem especially heavy
It wants.
It is to be colonized in plant root that Kloepper and Schroth defined plant growth-promoting rhizobacteria (PGPR) in 1978 for the first time
Beneficial soil bacteria.PGPR takes part in every biological activity of soil ecosystem, by improve nutrition utilizability (Gu
Nitrogen phosphorus decomposing etc.), improve hormone yield, the harmful microorganism of competition, the promotion of symbiosis etc., make it have dynamic nutrition
Conversion and sustainable crop production (Paul etc., 2005；Ai Wenqin etc., 2018).It is many studies have shown that relevant to root a variety of
Microorganism adapts to playing pass important role to promoting plant salt marsh.PGPR passes through production auxin (IAA), gibberellin
(GAs) and some unknown factors, it will lead to the increase of root long, root surface area and tip of a root quantity, thus promote the absorption of nutrient,
So as to improve health status of the plant under stress conditions.Pressure in terms of Saline soil agriculture can be mitigated using these microorganisms itself
Power, and promote the benign development of sustainable agriculture.
Currently, related PGPR improves solonchak, the research work for promoting plant salt endurance still in its infancy, mainly concentrate
It is planted in pseudomonas (Pseudomonas spp.), Bacillus (Bacillus spp.) etc., and to them in adjustment
Object Endogenous hormone balance, ionic homeostasis, anti-oxidant and photosynthesis etc. have done some mechanistic discussions.And about tomato
The introduction of the plant growth-promoting rhizobacteria of salt tolerant is seldom.
Summary of the invention
It is an object of the invention to solve above-mentioned the deficiencies in the prior art, provide a kind of salt tolerant growth-promoting bacteria strain Y4 and its
Using.
Technical solution
The present invention screens a kind of salt tolerant growth-promoting bacteria strain Y4, and classification naming is olive Halomonas (Halomonas
Olivaria), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address are as follows: Beijing
No. 3 Institute of Microorganism, Academia Sinica, institute of Chaoyang District North Star West Road 1, postcode: 100101, the deposit date is Mays 5 in 2019
Day, deposit number is CGMCC NO.17707.
The 16S rDNA nucleotide sequence of the salt tolerant growth-promoting bacteria strain Y4 is as shown in SEQ ID NO.1.
The salt tolerant growth-promoting bacteria strain Y4 is isolated from Yancheng rape rhizosphere soil, weighs Yancheng rape rhizosphere soil sample
(related root system of plant) is added distilled water and prepares soil supension, soil supension is transferred in the LB Liquid Culture containing 2%NaCl
In base, with 30 DEG C of constant temperature incubations of 200r/min revolving speed for 24 hours after, with the inoculum concentration of 1:100, transferred again in 4%NaCl content
Cultivated in LB culture medium for 24 hours, 30 DEG C of shaken cultivations for 24 hours after, then transfer in the LB culture medium of 6%NaCl content, 30 DEG C of oscillations
After culture for 24 hours, by gained inoculum 106Dilution spread, the well-grown single colonie of picking, that is, screen salt tolerant Promoting bacteria
Bacterial strain Y4.
Above-mentioned salt tolerant growth-promoting bacteria strain Y4 promotes the application of tomato growth in salt stress.
A kind of tomato growth-promoting agent containing above-mentioned salt tolerant growth-promoting bacteria strain Y4, the preparation method comprises the following steps: Y4 is inoculated in LB liquid
Culture medium, 180rpm, at 30 DEG C culture to OD600 be 1 (i.e. 108CFU/mL), bacterium solution is obtained, by bacterium solution turning with 6000rpm
Speed centrifugation 10min, abandons supernatant, reinjects isometric dd water, after oscillation is resuspended, is centrifuged 10min with the revolving speed of 6000rpm, abandons
Supernatant is repeated 2 times, up to tomato growth-promoting agent after resuspension.
Above-mentioned tomato growth-promoting agent in salt stress for promoting the application of tomato growth, application method are as follows: by tomato growth-promoting
Agent is injected in tomato seedling rhizosphere soil, and every plant of tomato seedling rhizosphere injects 10mL tomato growth-promoting agent.
Beneficial effects of the present invention: the present invention provides a kind of salt tolerant growth-promoting bacteria strain Y4, inoculating strain Y4 can be coerced in salt
The salt tolerant growth-promoting ability that tomato is significantly improved under compeling, is made tomato growth-promoting agent for the bacterial strain, is then injected into tomato seedling rhizosphere soil
In earth, the plant type, plant height and stem thickness of tomato can be significantly increased, remarkably promotes the dry-matter accumulation of tomato seedling, and to tomato children
The root long of seedling, surface area, root volume, tip of a root number have facilitation, salt damage phenomenon are slowed down, to promote tomato seedling
Growth, helps to improve the salt tolerance of tomato, reduces the use of chemical fertilizer.
Specific embodiment
It invents in order to better understand, below with reference to the embodiment content that the present invention is furture elucidated, but it is of the invention interior
Appearance is not restricted to following embodiment.In following embodiments, the tomato material of test is tomato " cooperation 903 " (Solanum
Lycopersicum) seed, from Shanghai tomato research institute.
Separation, the screening and identification of 1 salt tolerant growth-promoting bacteria strain Y4 of embodiment
It weighs Yancheng 2.0g rape rhizosphere soil sample (related root system of plant) and is placed in the conical flask equipped with 100mL distilled water
Interior, after being sealed with sealed membrane, 30 DEG C of constant temperature oscillations are for 24 hours to get soil supension in 200r/min shaking table.It is outstanding to draw 1mL soil
Liquid switching in the 100mL LB liquid medium containing 2%NaCl, with 30 DEG C of constant temperature incubations of 200r/min revolving speed for 24 hours after, still with
The inoculum concentration of 1:100 transfers cultivate for 24 hours in the LB culture medium of 4%NaCl content again, 30 DEG C of shaken cultivations for 24 hours after, then
Transfer in the LB culture medium of 6%NaCl content, 30 DEG C of shaken cultivations for 24 hours after, by gained inoculum 106Dilution spread,
The well-grown single colonie of picking screens salt tolerant growth-promoting bacteria strain Y4.
The identification of 2 salt tolerant growth-promoting bacteria strain Y4 of embodiment
1. DNA of bacteria extracts
The Bacteria Culture that embodiment 1 is separated and filtered out draws 1mL bacterium solution to 1.5mL centrifuge tube to logarithmic phase, first with
30min boiling water bath inactivation, then place it in centrifuge with 1000rpm be centrifuged 5min, gained supernatant and for DNA of bacteria extract
Liquid.
2. the PCR amplification of bacterial strain 16s rDNA
It is template by mentioned DNA of bacteria, amplimer uses bacterial universal primers, it may be assumed that forward primer U8-27 (F) 5 '-
AGAGTTTGATCCTGGCTCA-3'；Reverse primer L1494-1514 (R) 5 '-GGTTACCTTGTTACGACTT-3 '.50μL
PCR amplification system: 21 μ L dd water, positive each 1 μ L of anti-primer, the 25 above-mentioned DNA extracting solutions of μ L R-Taq mix, 2 μ L.In order plus
Enter each substance in above-mentioned system in 0.5mL PCR pipe, mix well, and 3~5s is centrifuged with 5000rpm.The above experiment behaviour
It is both needed to complete in super-clean bench, experiment used tool, consumptive material etc. are both needed to stringent sterilization.Amplified reaction program: 1. 94 DEG C of pre- changes
Property 2min, 2. 94 DEG C of denaturation 30s, 3. 52 DEG C of annealing 30s, 4. 72 DEG C of extension 1.5min, step 2.~4. carry out 30 circulations after,
System continues to extend 10min at 72 DEG C, and amplified production is stored in 4 DEG C.
Pcr amplification product 1% Ago-Gel (containing 0.01%GelRed) electrophoresis detection, with DNA Marker 2000
As reference, it is placed in 100V constant pressure electrophoresis 30min in 1 × TAE buffer.Then PCR is observed under gel imaging system to expand
Increase product band.
3. bacterial strain 16s rDNA sequencing and strain idenfication
It successful PCR product will be expanded send and be sequenced by Nanjing Jin Weizhi biotech firm, and by gained 16S rDNA sequence
Column are uploaded to EzBioCloud (https: //www.ezbiocloud.net/) and carry out sequence analysis, identify that the bacterial strain is olive
Olive Halomonas (Halomonas olivaria), is named as Y4.The bacterial strain Y4 screened is subjected to culture presevation, existing preservation
In China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address are as follows: BeiChen West Road, Chaoyang District, BeiJing City 1
Number No. 3 Institute of Microorganism, Academia Sinica, institute, postcode: 100101, the deposit date is on May 5th, 2019, deposit number was
CGMCC NO.17707。
3 salt tolerant growth-promoting bacteria strain Y4 of embodiment tests the salt tolerant growth-promoting of tomato
Preparation tomato growth-promoting agent: being inoculated in LB liquid medium for Y4,180rpm, at 30 DEG C culture to OD600 be 1 (i.e.
108CFU/mL), bacterium solution is obtained, bacterium solution is centrifuged 10min with the revolving speed of 6000rpm, supernatant is abandoned, reinjects isometric dd water,
After oscillation is resuspended, 10min is centrifuged with the revolving speed of 6000rpm, supernatant is abandoned, is repeated 2 times, up to tomato growth-promoting agent after resuspension.
Experimental design: in the same size, full tomato seeds are selected with 5% NaClO solution surface and sterilize 10min, are used
Deionized water repeated flushing seed is multiple, is placed in deionized water and soaks seed for 24 hours, then places it in the culture dish for being covered with wet gauze
On, 27 DEG C of vernalization 2d.It chooses the long consistent seed of bud to be seeded in plastic tub (basin high 17cm, bore 23cm), cultivation matrix is
Peat soil and vermiculite (volume ratio 2:1).Every basin broadcasts 6, and watering, thinning is when seedling length to 31 heart of leaf to ensure Qi Miao, seedling
Different disposal is transferred to when length to 41 heart of leaf.Experiment sets 0,2.5 ‰, 5 ‰ NaCl and handles 3 levels (with 80 DEG C of drying dry weights of matrix
On the basis of), 6 processing groups are set altogether, it may be assumed that (1) the not additional tomato growth-promoting agent (control group) of not additional NaCl+；(2) additional tomato
Growth-promoting agent；(3) additional 2.5 ‰ NaCl；(4) the additional tomato growth-promoting agent of additional 2.5 ‰ NaCl+；(5) additional 5 ‰ NaCl；(6) outside
Add the additional tomato growth-promoting agent of 5 ‰ NaCl+.Each processing group is poured by the NaCl solution of setting saturation, then with distilled water rinse, is made
It is uniform in matrix to obtain additional salinity, control is poured with distilled water, and the processing group of additional tomato growth-promoting agent is the 2nd day after salt treatment
With tomato growth-promoting agent pouring root, every plant of seedling rhizosphere injects 10mL tomato growth-promoting agent.Each 7 basin of processing group is tested, is adopted after handling 15d
Sample carries out index determining.Entire incubation is 16h:8h round the clock, and daylight degree is dysprosium lamp 10000Lux, temperature: daytime 25
± 5 DEG C and 20 ± 5 DEG C of night, relative humidity: daytime 50% ± 10% and night 60% ± 6%.
Experiment test:
1. chlorophyll content and photosynthetic parameters measurement
Daytime, SPAD chlorophyll meter (8SPAD502-plus, China) measures the new fully expanded leaves leaf of tomato in a handheld
Chlorophyll contents, 7 repetitions of each processing.It is newly complete with portable photosynthetic instrument (Li-COR, the U.S.) the measurement tomato of Li-6400 simultaneously
Net Photosynthetic Rate (Pn), the stomatal conductance (Gs), intercellular CO of leaf is unfolded2Concentration (Ci) and transpiration rate (Tr).Measurement uses red
Blue-light source leaf chamber, 25 ± 3 DEG C of temperture of leaves, intensity of illumination is 1000 μm of ol m-2s-1, 500 μm of ol s of airflow rate-1.Test result is shown in
Table 1:
Table 1
As can be seen from Table 1, under salt stress, tomato seedling Net Photosynthetic Rate (Pn) is obviously suppressed (P < 0.05),
Under 2.5 ‰ and 5 ‰ NaCl processing, 57% and 66% is reduced respectively than compareing.Under non-salt stress, tomato growth-promoting agent be can be improved kind
Eggplant seedling Pn 76%.Under 2.5 ‰ NaCl salinity, tomato growth-promoting agent processing plant Pn does not apply the raising of tomato growth-promoting agent
81%；Under 5 ‰ NaCl salinity, the Pn ratio of tomato growth-promoting agent processing does not apply the raising 123% of tomato growth-promoting agent.2.5 ‰ Hes
Plant stomatal conductance (Gs) is respectively than reducing by 67% and 92% under 5 ‰ NaCl salinity.Under salt stress, tomato growth-promoting agent is remarkably promoted
The Gs of plant leaf, under 2.5 ‰ NaCl salinity, the plant Gs ratio for applying the processing of tomato growth-promoting agent does not apply the increasing of tomato growth-promoting agent
Add 50%；Under 5 ‰ NaCl salinity, the Gs ratio of tomato growth-promoting agent processing does not apply the raising 500% of tomato growth-promoting agent.In the non-salt side of body
Under compeling, the plant intercellular CO of tomato growth-promoting agent processing2Concentration (Ci) and contrast difference be not significant (P < 0.05).2.5 ‰ NaCl salt
Under degree, the plant leaf Ci ratio of tomato growth-promoting agent processing does not apply the increase by 18% of tomato growth-promoting agent processing；5 ‰ NaCl salinity
Under, the plant leaf Ci ratio of tomato growth-promoting agent processing does not apply the increase by 228% of tomato growth-promoting agent processing, 2.5 ‰ and 5 ‰ salinity
Lower transpiration rate (Tr) is respectively than control decline 46% and 87%.Apply tomato growth-promoting agent and significantly improve plant Tr, wherein is non-salt
Under the conditions of tomato growth-promoting agent handle tomato leaf Tr ratio control increase by 155%；Under 2.5 ‰ NaCl salinity, apply tomato growth-promoting
Plant Tr ratio under agent processing does not apply the increase by 110% of tomato growth-promoting agent processing；Under 5 ‰ NaCl salinity, at tomato growth-promoting agent
The plant Tr ratio of reason does not apply the increase by 141% of tomato growth-promoting agent processing.
2. Root morphology is analyzed
Root system is scanned using Epson scanner (1640XL, China) and WinRHIZO analysis system software
(LA1600+, Canada) is analyzed, and the indexs such as total root long, root surface area, root volume, tip of a root number are obtained.Test result is shown in Table
2:
Table 2
As can be seen from Table 2, salt stress significantly suppresses tomato seedling root growth, and the higher inhibiting effect of salinity is more
By force；Under non-salt stress, the total root long for applying tomato growth-promoting agent improves 15.24% compared with the control；In 2.5 ‰ and 5 ‰
Under NaCl salinity, applies tomato growth-promoting agent and the total root long of tomato seedling is respectively increased compared with not applying the processing of tomato growth-promoting agent
61.45% and 62.17%；Under non-salt stress, the root surface area for applying the processing of tomato growth-promoting agent improves compared with the control
9.89%；In 2.5 ‰ and 5 ‰ NaCl salinity, application tomato growth-promoting agent is to tomato seedling root surface area compared with not being inoculated with processing
74.14% and 26.13% has been respectively increased；Under non-salt stress and 5 ‰ NaCl salinity, tomato growth-promoting agent is to tomato seedling root
Volume does not make significant difference, and when 2.5 ‰ NaCl salinity, and root volume is improved compared to not applying tomato growth-promoting agent processing group
116.67%；In terms of the promotion to tip of a root number, in non-salt stress, no facilitation, and 2.5 ‰ and 5 ‰ NaCl salinity
Under, tip of a root number improves 35.50% and 66.67% than not applying tomato growth-promoting agent processing group respectively.
3. tomato plant height, stem thickness, dry weight and root/shoot ratio
The whole strain of tomato seedling is taken out from matrix, is cleaned, is blotted surface moisture with blotting paper, tomato is divided into overground part
Point and under ground portion, use the rigid ruler of scale to measure plant height, stem thickness.Place the material in 105 DEG C of baking ovens water-removing 15min, then at 60 DEG C
Drying to constant weight for baking oven, weighs dry weight.With the dry weight of aerial part and under ground portion ratio, root/shoot ratio (R/S) is obtained, it may be assumed that
Root/shoot ratio (%)=(root dry weight/overground part dry weight) × 100
Test result is shown in Table 3:
Table 3
As can be seen from Table 3, compared with non-salt treatment, 2.5 ‰ and 5 ‰ NaCl processing plant height declines 20% He respectively
45%；And it is non-salt under the conditions of, tomato growth-promoting agent processing plant plant height than control increase by 63%；Under 2.5 ‰ NaCl stress, apply
Growth by 47% of the plant height for adding tomato growth-promoting agent to handle than not applying the processing of tomato growth-promoting agent；Compared with salt is not added, 2.5 ‰ Hes
It is respectively 29% and 39% that 5 ‰ NaCl, which handle stem thickness decline, and under non-salt treatment, the plant stem thickness of tomato growth-promoting agent processing is than control
Increase by 28%；Under 2.5 ‰ NaCl stress, the stem thickness of tomato growth-promoting agent processing increases by 49% than not being inoculated with processing；5 ‰ NaCl stress
Under, increase by 36% of the tomato seedling stem thickness of tomato growth-promoting agent processing than not applying the processing of tomato growth-promoting agent.
2.5 ‰ and 5 ‰ NaCl processing plant than non-salt treatment reduction respectively 65% and 77%, under the conditions of non-salt,
The tomato overground part dry weight of tomato growth-promoting agent processing improves 139% than control；2.5 ‰ NaCl stress under, tomato growth-promoting agent to kind
Eggplant seedling overground part dry weight increases by 428% than control；Under 5 ‰ NaCl stress, the plant of tomato growth-promoting agent processing increases than control
503%.Under 2.5 ‰ and 5 ‰ NaCl stress, tomato seedling root dry weight reduces 48% and 67% than no salt added respectively.Non-
Under salt stress is horizontal, the tomato seedling root dry weight of tomato growth-promoting agent processing increases by 100% than control；Under 2.5 ‰ NaCl stress,
The seedling root dry weight of tomato growth-promoting agent processing increases by 172% than control；Under 5 ‰ NaCl stress, the plant of tomato growth-promoting agent processing
Strain root dry weight increases by 243% than control；Difference trend and overground part is similar between the processing of whole plant dry weight；In non-salt condition
Under, the plant root cap of tomato growth-promoting agent processing is lower than control 17.75%；Under 2.5 ‰ NaCl stress, kind of tomato growth-promoting agent processing
Eggplant seedling root/shoot ratio decline 45.11%；Under 5 ‰ NaCl stress, the tomato seedling root/shoot ratio decline of tomato growth-promoting agent processing
44.70%.
4. Mineral Elements Content measures
Above-mentioned drying plant sample is ground to and crossed 40 mesh stainless steels sieve, obtains stand-by dry-eye disease.With reference to Bao Shidan (Bao Shidan
.3 editions [M] Chinese agriculture publishing houses of Soil Analysis in Agricultural Chemistry, 2000) method is measured: the survey of plant K, Na, Ca, Mg, Fe
Surely HNO is used3Resolution will disappear and use ICP Atomic Emission Spectrometer AES (Agilent Technologies after boiling dilution filtering
710, Australia) carry out constituent content mensure.Test result is shown in Table 4:
Table 4
As can be seen from Table 4, as the aggravation of salt stress, Tomato Seedling Leaves Na content obviously rise, K, Ca, Mg contain
Amount declines, and under non-salt stress, tomato growth-promoting agent processing group has not significant impact blade Na and K content compared with the control group,
But under 2.5 ‰ and 5 ‰ NaCl salinity, the blade Na content of tomato growth-promoting agent processing, which is compared, does not apply the processing of tomato growth-promoting agent
50% and 23% is reduced respectively, and 39% and 68% has been respectively increased in K content.Under non-salt stress and 5 ‰ NaCl salinity, tomato promotees
For the blade Ca content of raw agent processing without significant change, and under 2.5 ‰ NaCl salinity, the blade Ca for being inoculated with Y4, which is compared, does not apply tomato
Growth-promoting agent processing improves 29%.Under non-salt stress, the processing of tomato growth-promoting agent influences less blade Mg content, and 2.5 ‰
Under 5 ‰ NaCl salinity, the blade Mg content of tomato growth-promoting agent processing, which is compared, does not apply being respectively increased for tomato growth-promoting agent processing
74% and 43%；Under non-salt stress, the blade Fe content of tomato growth-promoting agent processing improves 23%, 2.5 ‰ and 5 ‰ NaCl salt
Under degree, tomato growth-promoting agent processing blade Fe content compare do not apply tomato growth-promoting agent processing 48% He has been respectively increased
52%.
Sequence table
<110>Agricultural University Of Nanjing
<120>a kind of salt tolerant growth-promoting bacteria strain Y4 and its application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1387
<212> DNA
<213>olive Halomonas (Halomonas olivaria)
<400> 1
gcgttaggct aaccacttct ggtgcagtcc actcccatgg tgtgacgggc ggtgtgtaca 60
aggcccggga acgtattcac cgtgacattc tgattcacga ttactagcga ttccgacttc 120
acggagtcga gttgcagact ccgatccgga ctgagaccgg ctttaaggga ttcgctgact 180
ctcgcaagct cgcagccctt tgtaccggcc attgtagcac gtgtgtagcc ctacccgtaa 240
gggccatgat gacttgacgt cgtccccacc ttcctccggt ttgtcaccgg cagtctcctt 300
agagttcccg acattactcg ctggcaaata aggacaaggg ttgcgctcgt tacgggactt 360
aacccaacat ttcacaacac gagctgacga cagccatgca gcacctgtct tacagttccc 420
gaaggcacac cagaatctct tccggcttct gtagatgtca agggtaggta aggttcttcg 480
cgttgcatcg aattaaacca catgctccac cgcttgtgcg ggcccccgtc aattcatttg 540
agttttaacc ttgcggccgt actccccagg cggtcgactt atcgcgttaa cttcgccaca 600
aagtgctcta ggcacccaac ggctggtcga catcgtttac ggcgtggact accagggtat 660
ctaatcctgt ttgctaccca cgctttcgca cctcagtgtc agtgtcagtc cagaaggccg 720
ccttcgccac tggtattcct cccgatctct acgcatttca ccgctacacc gggaattcta 780
ccttcctctc ctgcactcta gcctgacagt tccggatgcc gttcccaggt tgagcccggg 840
gctttcacaa ccggcttatc aagccaccta cgcgcgcttt acgcccagta attccgatta 900
acgcttgcac cctccgtatt accgcggctg ctggcacgga gttagccggt gcttcttctg 960
cgagtgatgt ctttcctaat gggtattaac cactaggcgt tcttcctcgc tgaaagtgct 1020
ttacaacccg agggccttct tcacacacgc ggcatggctg gatcagggtt ccccccattg 1080
tccaatattc cccactgctg cctcccgtag gagttcgggc cgtgtctcag tcccgatgtg 1140
gctgatcatc ctctcagacc agctacggat cgttgccttg gtgagccttt acctcaccaa 1200
ctagctaatc cgacataggc tcatccaata gcgggagccg aagccccctt tctcccgtag 1260
gacgtatgcg gtattagcct gggtttcccc aggttatccc ccactaccgg gcagattcct 1320
atgcattact cacccgtccg ccgctcgtca gcatctagca agctagatct gttaccgctc 1380
gactgca 1387
Claims (7)
1. a kind of salt tolerant growth-promoting bacteria strain Y4, which is characterized in that it is general that it is preserved in China Committee for Culture Collection of Microorganisms
Logical microorganism center, the deposit date is on May 5th, 2019, deposit number was CGMCC NO.17707.
2. salt tolerant growth-promoting bacteria strain Y4 as described in claim 1, which is characterized in that the 16S of the salt tolerant growth-promoting bacteria strain Y4
RDNA nucleotide sequence is as shown in SEQ ID NO.1.
3. the application that salt tolerant growth-promoting bacteria strain Y4 as claimed in claim 1 or 2 promotes tomato growth in salt stress.
4. a kind of tomato growth-promoting agent containing salt tolerant growth-promoting bacteria strain Y4 as claimed in claim 1 or 2.
5. the preparation method of tomato growth-promoting agent described in claim 4, which is characterized in that Y4 is inoculated in LB liquid medium,
180rpm, culture to OD600 is 1 at 30 DEG C, obtains bacterium solution, bacterium solution is centrifuged 10min with the revolving speed of 6000rpm, abandons supernatant,
Isometric dd water is reinjected, after oscillation is resuspended, 10min is centrifuged with the revolving speed of 6000rpm, supernatant is abandoned, is repeated 2 times, after resuspension
Up to tomato growth-promoting agent.
6. tomato growth-promoting agent described in claim 1 in salt stress for promoting the application of tomato growth.
7. application as claimed in claim 6, which is characterized in that the application method are as follows: by tomato growth-promoting agent injection tomato children
In seedling rhizosphere soil, every plant of tomato seedling rhizosphere injects 10mL tomato growth-promoting agent.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109988738A (en) * | 2019-05-22 | 2019-07-09 | 南京农业大学 | A kind of salt tolerant growth-promoting bacteria strain B9 and its application |
| CN113943684A (en) * | 2021-11-24 | 2022-01-18 | 青岛理工大学 | Tender haloplankton and application thereof in production of salinization farmland |
| CN115895954A (en) * | 2022-11-15 | 2023-04-04 | 江苏省中国科学院植物研究所 | Pseudomonas laurophilus sulfate CNBG-PGPR-8 and application thereof |
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| AGNES AMOURIC 等: "Halomonas olivaria sp. nov., a moderately halophilic bacterium isolated from olive-processing effluents", 《INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY》 * |
| AISHA WAHEED QURASHI 等: "BACTERIAL EXOPOLYSACCHARIDE AND BIOFILM FORMATION STIMULATE CHICKPEA GROWTH AND SOIL AGGREGATION UNDER SALT STRESS", 《BRAZILIAN JOURNAL OF MICROBIOLOGY》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988738A (en) * | 2019-05-22 | 2019-07-09 | 南京农业大学 | A kind of salt tolerant growth-promoting bacteria strain B9 and its application |
| CN109988738B (en) * | 2019-05-22 | 2022-03-11 | 南京农业大学 | Salt-tolerant growth-promoting bacterial strain B9 and application thereof |
| CN113943684A (en) * | 2021-11-24 | 2022-01-18 | 青岛理工大学 | Tender haloplankton and application thereof in production of salinization farmland |
| CN113943684B (en) * | 2021-11-24 | 2024-02-13 | 青岛理工大学 | Salmonella pinnata and application thereof in salinized farmland production |
| CN115895954A (en) * | 2022-11-15 | 2023-04-04 | 江苏省中国科学院植物研究所 | Pseudomonas laurophilus sulfate CNBG-PGPR-8 and application thereof |
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