CN1100200A - Capillary electrophoretic sampling device without electric discrimination - Google Patents
Capillary electrophoretic sampling device without electric discrimination Download PDFInfo
- Publication number
- CN1100200A CN1100200A CN 93117119 CN93117119A CN1100200A CN 1100200 A CN1100200 A CN 1100200A CN 93117119 CN93117119 CN 93117119 CN 93117119 A CN93117119 A CN 93117119A CN 1100200 A CN1100200 A CN 1100200A
- Authority
- CN
- China
- Prior art keywords
- electrode
- volt
- sample
- sampling device
- voltage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Electrostatic Separation (AREA)
Abstract
The capillary electrophoretic sampling device features no electric discrimination and simple structure. The third electrode E3 is provided between electrode E1 and E2. When E3 is positive, E2 is negative and an electric cataphoresis takes place in capillary between E2 and E3 in the direction from E3 to E2. As the capillary is an integral, the cataphoresis generates pumping action to suck sample in E1 into capillary.
Description
Nothing electricity discrimination novel capillary electrophoresis sampling device belongs to the capillary electrophoresis in the analytical chemistry.
Capillary Electrophoresis is the separation that developed rapidly in recent years, analyzes new technology.It uses the kapillary of internal diameter 20-100 micron, be up to the electrophoretic separation of carrying out sample under 50,000 volts of voltages, can realizing efficient (every meter tens to millions of theoretical trays) easily, fast (generally only needing a few minutes), it is sensitive that (when using UV-detector, minimum detectable quantity reaches 10
-14～10
-16Mol) and the compartment analysis of robotization.Because capillary electrophoresis tube column volume minimum (generally only counting microlitre), the sample feeding amount is for receiving upgrading (10
-9L), so, require very high to sampling technique.At present, generally use following three kinds of sample injection methods, that is:
(1) electrokinetic injection (being called moving electric sample introduction again, electromigration injection etc.) utilizes the electromigratory acting in conjunction of electroosmotic flow and ion, and sample is imported capillary electrophoresis system.This method is easy, but has electricity to discriminate against phenomenon, causes the difference of the ion sample size that specific charge is different in the sample.
(2) fluid mechanics sample introduction: comprise that with vacuum, modes such as pressure or potential difference are introduced electrophoresis system with sample.Do not have electricity to discriminate against phenomenon, but complex structure and control are difficult.
(3) micro-syringe sample introduction: because of volume is too little, be difficult to control, adopt the split sampling mode usually.Requirement has high-precision physical construction, and difficult control.
On the commodity capillary electrophoresis apparatus, generally adopt electrokinetic injection and fluid mechanics sample introduction dual mode simultaneously.
The objective of the invention is to look phenomenon, complex structure and unmanageable shortcoming and propose a kind of capillary electrophoretic sampling device that adopts three-electrode system in order to overcome electricity of the prior art.Simple mechanism such as Fig. 1.
Capillary electrophoretic sampling device of the present invention as can be seen from Figure 1 removes electrode E
1And E
2Outer third electrode E in addition
3, three electrodes are connected by capillary column C.A detection window W is positioned at E
1And E
3Between, at E
1And E
2Between adjustable main high-voltage DC power supply V is housed
1, V
1Can produce 1～50000 volt of voltage is electrophoretic voltage, at E
2With E
3Between adjustable auxilliary high-voltage DC power supply V is housed
2, V
2Can produce 1～5000 volt of voltage is sample introduction voltage, usually electrode E
3Apart from E
2Be 1-100 centimetre, be preferably 10-30 centimetre, detection window W distance electrode E
1Be 10-150 centimetre, be preferably 10～30 centimetres, other ultraviolet or other suitable detector D of being equipped with of detection window W.
When operating, at slot electrode E
1Sample cell is placed at the place, at electrode E
2And E
3Between apply voltage V
2, V
2Be the 100-5000 volt, be preferably the 600-3000 volt, E
3Be positive pole, E
2Be negative pole, at kapillary E
2-E
3Just can produce electroosmotic flow in the section, its direction is E
3→ E
2Because E
1-E
2Between kapillary be an integral body, then E
2-E
3Between electroosmotic flow just can produce pump-absorb action, with E
1The sample at place sucks in the kapillary, finishes sample introduction.When system geometric parameter fixedly the time, sample size with apply voltage, voltage action time and sample solution concentration are proportional.After finishing sample introduction, with E
1The sample cell at place changes the electrode liquid pool into, at E
1-E
2Between apply voltage 100-50000 volt, preferable 100-30000 volt makes sample carry out capillary electrophoresis analysis by ultraviolet detection, carries out quantitative Analysis with record peak height or peak area value, electrode solution is an electrolyte aqueous solution usually.
Above input mode can be used for the various clastotypes of Capillary Electrophoresis, but inapplicable to capillary gel electrophoresis.In capillary gel electrophoresis, can utilize E
1, E
2Two electrodes or E
1, E
3Two electrodes carry out common electrokinetic injection operation.
Electrode E
1, E
2Identical with general capillary electrophoresis, and preferably be on the equal height, avoiding because of flowing that difference in height is brought, third electrode is in E
1And E
2Between, preferably adopt vertical vertical mounting means.
The capillary column of this system can use internal diameter to be the 20-200 micron usually, and length is 10-200 centimetre elastic quartz capillary tube, E
1-E
3Between the kapillary kapillary that also can use other insulating material to make, electrode E
1And E
2With used identical in the common Capillary Electrophoresis.Electrode E
3Have special construction, its citation form can be fixed, can also be dismountable, their difference only is to dock the difference of sleeve pipe, the sealing of fixed employing tackifier, and detachable be to utilize elasticity polymeric seal circle to replace tackifier, thereby can repeated multiple times change E
1-E
3Between kapillary.Fig. 2 has illustrated stationary structure figure, two sections elastic quartz capillary tube (2,10) butt joints that polish fracture, the interface overcoat is sealed (6) with one section butt joint sleeve pipe (7) and with tackifier with sleeve pipe two ends and slit capillaceous, the kapillary that connects is packed in the slot electrode (5), and its lower end adds elastic rubber fitting (8) and compresses in case electrode solution leaks with slot electrode lower cover (9).Wire electrode (1) passes loam cake (3) and inserts slot electrode (5), and whole third electrode is preferably vertically installed, and charges into electrode solution during use in the slot electrode and makes it not have the butt joint sleeve pipe, and electrode solution enters slot electrode from (11), and draws by liquid outlet (4).
Quartz capillary is an internal diameter 20-200 micron elastic quartz capillary tube; The butt joint sleeve pipe can be used controllable bore diameter glass or materials such as controllable bore diameter pottery hollow fiber, macromolecular material ultrafiltration hollow fiber or hollow amberplex; Tackifier can adopt epoxy resin, tackifier such as organic silicon adhesive, but they must be for a long time when solution electrode soaks, be not destroyed and do not discharge soluble impurity, wire electrode can be selected platinum filament, spun gold, graphite spine or other suitable electrode material for use, slot electrode main body, upper and lower covers etc. can adopt organic glass, and polystyrene or other any engineering plastics or glass are made; The lower cover seal gasket can be used natural rubber, silicon rubber, and fluororubber or other organic elastomer are made.Do not have the novel capillary electrophoresis sampling device of electricity discrimination and can finish sample introduction yet by the system that three above multi-electrodes are formed.Utilize novel three electrode capillary electrophoretic sampling devices of the present invention, the electricity that produces in the time of can avoiding using the electric power sample introduction because of the different ion of specific charge in the sample is discriminated against phenomenon, reach the effect of the balanced sample introduction identical with the fluid mechanics sampling system, it is easy to have sample introduction simultaneously, the advantage that is easy to control.For example, be sample with 1: 1 the mixed solution of weight ratio of dansyl base lysine and dansyl base glutamic acid, when electrokinetic injection, dansyl base lysine is 1: 0.620 with the ratio of dansyl base glutamic acid peak height; During with three electrode sample introductions then is 1: 0.951, and the electricity the when latter does not show the common electric sample introduction is discriminated against phenomenon.
With long 50 centimetres, 75 microns of internal diameters, the elastic quartz capillary tube that external diameter is 375 microns is equipped with 100-30000 and lies prostrate adjustable direct supply (V
1) and 1-3000 lie prostrate adjustable direct supply (V
2) two power supplys, at distance E
220 centimeters are installed third electrode E
3, constitute three electrode capillary electrophoresis systems.At distance E
120 centimeters are opened a detection window W, and D detects with the 254nm UV-detector.Shell material is controllable bore diameter glass (micropore diameter 30nm) hollow fiber, and its internal diameter is 500 microns.Quantitative with the peak height value, the pen recorder, record.
When being sample (1.5mg/ml) with the dansyl alanine, sample introduction voltage V
2=3000 volts, electrophoretic voltage V
1=15000 volts, sample injection time t=7 second, the sampling given figure is that n=8 promptly measures 8 times, and its peak height mean value is 6.07cm, relative standard deviation RSD=1.75%.
Experimental provision and condition are with example (1), but change sample introduction voltage, to measure the relation of sample size and sample introduction voltage.H(centimetre of peak height value) linear to sample introduction voltage.
| V (volt) 600 1,200 1,800 2,400 3000 | ||||||
| H(cm) 2.50 3.28 4.06 4.68 5.10 (electrophoretogram peak heights) |
Experimental provision and condition are with example 1, but sample is made into variable concentrations, measure the peak height value h on the electrophoretogram.
| Concentration (mg/ml) 2.0 1.5 1.0 0.8 0.4 | ||||||
| ?h(cm） 85.6 62.8 49.0 41.0 19.3 |
Embodiment 4
Experimental provision and condition are with example 1, but the change sample injection time is measured the peak height value on the electrophoretogram.
| Time (t) seconds 1.22 2.43 3.69 4.87 5.92 7.14 8.33 9.73 11.03 | ||||||||||
| h(cm) 1.92 2.37 3.00 3.32 3.52 4.35 4.52 4.75 5.12 |
Its experiment condition and device are sample with example 1 with 1: 1 the mixed solution of weight ratio of dansyl base lysine and dansyl base glutamic acid, and when electrokinetic injection, dansyl base lysine is 1: 0.620 with the ratio of dansyl base glutamic acid peak height; During with three electrode sample introductions then is 1: 0.951, and the electricity the when latter does not show sample introduction is discriminated against phenomenon.
Description of drawings
Fig. 1, nothing electricity are discriminated against novel capillary electrophoresis sampling device synoptic diagram
E
1First electrode
E
2Second electrode
E
3Third electrode
V
1Adjustable main high-voltage DC power supply
V
2Adjustable auxilliary high-voltage DC power supply
C: kapillary
W: detection window
D: detecting device
Fig. 2, third electrode structural drawing
(1) wire electrode (2) elastic quartz capillary tube
(3) loam cake (4) liquid outlet
(5) slot electrode (6) seam seal gum
(7) butt joint sleeve pipe (8) elastic rubber fitting
(9) lower cover (10) elastic quartz capillary tube
(11) inlet
Claims (5)
1, a kind of novel capillary electrophoresis sampling device that does not have the electricity discrimination is characterized in that:
(1) system comprises E
1, E
2And E
3Three electrodes, kapillary, main high-voltage power supply, auxilliary high-voltage power supply, sample detection window, and detector system.
(2) special construction of third electrode
(3) connection of whole device and mode of operation are at slot electrode E
1Sample cell is placed at the place, at electrode E
2, E
3Be positive pole, E
2Be negative pole, at kapillary E
2-E
3Produce electroosmotic flow in the section, its direction is E
3→ E
2, because E
1→ E
2Between kapillary be an integral body, then E
2-E
3Between electroosmotic flow produce pump-absorb action with E
1The sample at place sucks in the kapillary, finishes sample introduction.
Described three electrodes contain electrode solution, and kapillary adopts elastic quartz capillary tube, main high-voltage power supply V
1The adjustable dc voltage V that produces
1Be the 1-50000 volt, auxilliary high-voltage power supply V
2The adjustable dc voltage V that produces
2Be the 1-50000 volt, other detecting device, for example the 254nm UV-detector D of placing of sample detection window W.
Described three electrodes and the arrangement mode and the position of installing each parts can be E
1And E
2Has sustained height, E
3Be vertical vertical installation, E
3Apart from E
2Be 1-100 centimetre, be preferably 10-30 centimetre, inspection hole W distance electrode E
1Be 10-150 centimetre, preferable 10-30 centimetre.
Described third electrode E
3Special construction be this electrode by slot electrode, two sections kapillaries of butt joint, butt joint sleeve pipe, the loam cake of slot electrode and lower cover, and pass the third electrode silk that loam cake inserts slot electrode and form.
2, nothing electricity according to claim 1 is discriminated against the novel capillary electrophoresis sampling device, it is characterized in that main high direct voltage V
1Be the 100-50000 volt, preferable 100-30000 volt, auxilliary DC voltage V
2Be the 100-5000 volt, preferable 600-3000 volt.
3, a kind of novel capillary electrophoresis sampling device that electricity is discriminated against that do not have according to claim 1 is characterized in that described third electrode E
3In contain fixable type and detachable butt joint sleeve pipe, the sealing of fixed employing tackifier, detachable employing elasticity polymeric seal circle sealing.Have an elastic rubber fitting and slot electrode lower cover to compress in the slot electrode lower end, the electrode solution that charges in the slot electrode need not crossed the butt joint sleeve pipe.
4, a kind of novel capillary electrophoresis sampling device that electricity is discriminated against that do not have according to claim 1 is characterized in that it is electrode material that described wire electrode can adopt platinum filament, spun gold, graphite spine.
5, a kind of novel capillary electrophoresis sampling device that electricity is discriminated against that do not have according to claim 3 is characterized in that described butt joint sleeve pipe can use materials such as controllable bore diameter glass, controllable bore diameter pottery, hollow fiber, polymer ultrafiltration hollow fiber, hollow amberplex.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93117119 CN1032608C (en) | 1993-09-07 | 1993-09-07 | Capillary electrophoretic sampling device without electric discrimination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93117119 CN1032608C (en) | 1993-09-07 | 1993-09-07 | Capillary electrophoretic sampling device without electric discrimination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1100200A true CN1100200A (en) | 1995-03-15 |
| CN1032608C CN1032608C (en) | 1996-08-21 |
Family
ID=4991858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93117119 Expired - Lifetime CN1032608C (en) | 1993-09-07 | 1993-09-07 | Capillary electrophoretic sampling device without electric discrimination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1032608C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548325A (en) * | 2016-01-30 | 2016-05-04 | 福州大学 | Capillary electrophoresis revolving shaft type liquid drop sample-introducing device and sample-introducing method thereof |
| CN108593748A (en) * | 2018-01-26 | 2018-09-28 | 南京溯远基因科技有限公司 | capillary and DNA sequencer |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006063508A1 (en) * | 2004-12-13 | 2006-06-22 | Accelergy Shanghai R & D Center | Method and system for treating ionic liquids by using capillary electrophoresis |
-
1993
- 1993-09-07 CN CN 93117119 patent/CN1032608C/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548325A (en) * | 2016-01-30 | 2016-05-04 | 福州大学 | Capillary electrophoresis revolving shaft type liquid drop sample-introducing device and sample-introducing method thereof |
| CN108593748A (en) * | 2018-01-26 | 2018-09-28 | 南京溯远基因科技有限公司 | capillary and DNA sequencer |
| CN108593748B (en) * | 2018-01-26 | 2024-04-30 | 南京溯远基因科技有限公司 | Capillary and DNA sequencer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1032608C (en) | 1996-08-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lu et al. | End-column electrochemical detection for inorganic and organic species in high-voltage capillary electrophoresis | |
| Jin et al. | Determination of diclofenac sodium by capillary zone electrophoresis with electrochemical detection | |
| Hempel | Strategies to improve the sensitivity in capillary electrophoresis for the analysis of drugs in biological fluids | |
| EP0475533B1 (en) | Method and apparatus for the introduction of a volume of at least one fluid in a tube, in particular suitable for capillary electrophoresis systems and method and apparatus for separating and/or analyzing a fluid material | |
| Jin et al. | Determination of bovine serum albumin by capillary zone electrophoresis with end-column amperometric detection at the carbon fiber microdisk array electrode | |
| EP0295942B1 (en) | On-column conductivity detector for microcolumn electrokinetic separations | |
| Chankvetadze et al. | Potential of flow‐counterbalanced capillary electrophoresis for analytical and micropreparative separations | |
| Jemere et al. | Microchip‐based capillary electrochromatography using packed beds | |
| Kašička et al. | Capillary zone electrophoresis with electroosmotic flow controlled by external radial electric field | |
| CN1306266C (en) | Device and method for leading-in sample into microcrystalline chip electrophoresis | |
| Kubáň et al. | A flow injection‐capillary electrophoresis system with high‐voltage contactless conductivity detection for automated dual opposite end injection | |
| US5223114A (en) | On-column conductivity detector for microcolumn electrokinetic separations | |
| Fanali et al. | Separation of enantiomers by on‐line capillary isotachophoresis‐capillary zone electrophoresis | |
| Eriksson et al. | Preparative capillary electrophoresis based on adsorption of the solutes (proteins) onto a moving blotting membrane as they migrate out of the capillary | |
| Li et al. | Quantitative capillary electrophoresis and its application in analysis of alkaloids in tea, coffee, coca cola, and theophylline tablets | |
| Rodríguez et al. | Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography | |
| US20240310326A1 (en) | Reusable cartridge for capillary electrophoresis | |
| CN1032608C (en) | Capillary electrophoretic sampling device without electric discrimination | |
| Stroink et al. | On‐line coupling of size‐exclusion chromatography and capillary zone electrophoresis via a reversed‐phase C18 trapping column for the determination of peptides in biological samples | |
| US5614073A (en) | Method and apparatus for detection of underivatized amines and amino acids utilizing end column addition of Ru(bpy)32+ | |
| Huang et al. | End‐column electrochemical detection for aromatic amines with high performance capillary electrophoresis | |
| CN102565255B (en) | PCEC (Pressurized Capillary Electrochromatography) ELSD (Evaporative Light-Scattering Detector) | |
| Fan et al. | Head‐column field‐amplified sample stacking in a capillary electrophoresis–flow injection system | |
| Jin et al. | A new method of quantification of pipemidic acid by capillary zone electrophoresis with end‐column amperometric detection | |
| Yao et al. | Quantitative sample injection for capillary electrophoresis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned | ||
| C20 | Patent right or utility model deemed to be abandoned or is abandoned |