CN110018266A - A kind of method of 48 kinds of amino acid of fast quantitative analysis - Google Patents

Abstract

The invention discloses a kind of methods of 48 kinds of amino acid of fast quantitative analysis.The present invention provides a kind of derivatization reagent combinations of the quantitative analysis of derivatization HPLC-MS/MS method detection amino acid content, including derivatization reagent A and derivatization reagent B；Wherein, derivatization reagent A is the mixed solution of normal propyl alcohol and 3- picoline, the mixed solution of methylene chloride, chloroformyl propyl ester and isooctane that derivatization reagent B is.And it further established the combination of stable isotope internal standard solution, sample-pretreating method and liquid chromatography-tandem mass spectrometry method based on the derivatization method.Derivatization reagent derivatization proposed by the present invention is high-efficient, and reagent toxicity is low；Sample size needed for method is few, processing is simple, quick, reproducible, and testing cost is low；It realizes and 22min has only been used to substantially reduce analysis time to the absolute quantification analysis of 48 kinds of amino acid, improve detection flux；With very big application value.

Description

A kind of method of 48 kinds of amino acid of fast quantitative analysis

Technical field

The present invention relates to technical field of biological, more particularly, to a kind of 48 kinds of amino acid of fast quantitative analysis Method.

Background technique

Amino acid is a kind of organic compound containing amino and carboxyl functional group, be protein basic composition unit and Metabolite is a kind of important biomarker.To amino acid in human body fluid (including blood, urine, cerebrospinal fluid, tissue fluid) Content is analyzed, can be used for disorder of amino acid catabolism class disease screening and auxiliary diagnosis and human nutrition situation assessment and Monitoring.

Disorder of amino acid catabolism class disease often results in multiple amino acid levels and changes, this proposes traditional analysis method Challenge is gone out, the ELISA method for once analyzing a compound is completely unsuitable for the analysis of this kind of compound.In addition, amino acid The presence of isomer further increases the difficulty of analysis.Since Most amino-acids are low molecular weight, highly polar chemical combination Object, currently, amino acid analysis is frequently with before column or post-column derivation-chromatography or Chromatography/Mass Spectrometry method are analyzed.

The amino acid analysis method clinically applied mainly includes ninhydrin derivatization method after ion-exchange chromatography-column (IEX), OPA/FMOC column front derivation-high performance liquid chromatography (HPLC), iTraq column front derivation-liquid chromatography tandem mass spectrometry (iTraq-LC-MS/MS) etc..But some defects are individually present in these methods, limit them in the popularization of clinical labororatory Using: IEX method grows (> 120min) there are data acquisition time and cannot be to the important biomolecules mark such as methionine and homotype citrulling The shortcomings that will object is separated；OPA/FMOC-HPLC method can only once analyze more than 20 kinds of common amino acids, data acquisition time ratio IEX method is fast, but a sample also needs 30min, and anti-matrix interference ability is poor, isomer separating capacity is poor, causes method special It is anisotropic not high；ITraq-LC-MS/MS method has many advantages, such as quick, sensitive, but iTraq reagent price used in the method is expensive, And the method is to sulfur-containing amino acid, such as methionine, cystine, cysteine, homocystine, the detection of homocysteine etc. There are poor repeatability, the shortcomings that the rate of recovery low (< 80%).

The early 1990s has scholar to propose to analyze amino acid using chloroformyl ester derivative-gas chromatography mass spectrometry, The method significantly improves method sensitivity and detection flux, but the method can not detect the amino acid containing guanidine radicals, such as citrulling, homotype Citrulling, arginine etc., and this kind of compounds (belong to disorder of amino acid catabolism in screening and diagnosis urea cycle disorder class disease Class disease) it is of great significance.EZ:Faast derivatization-HPLC-MS/MS the method that Phenomenex company releases detects plasma ammonia Base acid is exactly the method developed on the basis of processing before above-mentioned chloroformyl ester derivatization, which can carry out guanidine radicals amino acid Analysis, but pretreatment process extracts the scheme of progress derivative reaction after amino acid, the plasma volume needed using solid phase extraction (100 μ L blood plasma) greatly, test procedure is more, cumbersome, to affect the method rate of recovery and accuracy；Secondly, Phenom Pyridine and chloroform in enex EZ:Faastt derivatization reagent have high toxicity.Again, Phenomenex EZ:Faast Only used 3 compounds as internal standard, cannot the amino acid different to molecular structure extracted in sample pretreatment process, be derivative Change difference caused by matrix effect, ion depression effect present in the difference reacted and data acquisition etc. to be calibrated, Absolute quantification analysis can not be carried out to all analytes.

Summary of the invention

The purpose of the invention is to overcome the deficiencies of the prior art and provide a kind of 48 kinds of amino acid of fast quantitative analysis Method.

The first purpose of the invention is to provide a kind of derivatization examinations of HPLC-MS/MS quantitative analysis detection amino acid Agent combination.

A second object of the present invention is to provide one kind¹³C₃The preparation method of the amino-acid ester compound of label.

Third object of the present invention is to provide a kind of internal standard solutions of derivatization HPLC-MS/MS method detection amino acid content Combination.

Fourth object of the present invention is to provide a kind of sample of derivatization HPLC-MS/MS quantitative analysis amino acid content The method of product pre-treatment.

Fifth object of the present invention is to provide a kind of methods of the analysis detection amino acid of derivatization HPLC-MS/MS method.

Sixth object of the present invention is to provide a kind of kits of derivatization HPLC-MS/MS method detection amino acid content.

7th purpose of the invention is to provide the derivatization reagent combination, the preparation method, the internal standard solution group Conjunction, the sample-pretreating method, the method or any application in detection amino acid of the kit.

8th purpose of the invention is to provide the derivatization reagent combination, the preparation method or the internal standard solution group Close any application in the kit kind for preparing derivatization HPLC-MS/MS method detection amino acid content.

To achieve the goals above, the present invention is achieved by the following technical programs:

The present invention provides a kind of derivatization examinations of the quantitative analysis of derivatization HPLC-MS/MS method detection amino acid content Agent combination, and further established based on the derivatization method stable isotope internal standard solution combination, sample-pretreating method and Liquid chromatography-tandem mass spectrometry method.

A kind of derivatization reagent combination of HPLC-MS/MS method analysis detection amino acid, including derivatization reagent A and derivative Change reagent B；Wherein, derivatization reagent A is the mixed solution of normal propyl alcohol and 3- picoline, the dichloromethane that derivatization reagent B is The mixed solution of alkane, chloroformyl propyl ester and isooctane.

Amino and carboxyl of this derivatization method based on amino acid, in aqueous phase system can rapidly with chloroformyl propyl ester and just Propyl alcohol reaction generates the principle of carboxylate.3- picoline is the catalyst of the reaction, and the addition of methylene chloride and isooctane has It is carried out conducive to reaction is mild, and improves reaction efficiency.

Preferably, the derivatization reagent combination can be used for quantitative analysis detection amino acid, amino-containing small molecule chemical combination The small molecule compound content of object or carboxyl.

Preferably, it is calculated with volume fraction, derivatization reagent A contains 70%~80% normal propyl alcohol and 20%~30% 3- picoline.

Preferably, in derivatization reagent A, the volume ratio of normal propyl alcohol and 3- picoline is 77:23.

Preferably, calculated with volume fraction, derivatization reagent B contain 65%~75% methylene chloride, 15%~20% Chloroformyl propyl ester and 20%~5% isooctane.

Preferably, in derivatization reagent B, the volume ratio of methylene chloride, chloroformyl propyl ester and isooctane is 71.6:17.4: 11。

This law uses chloroformyl propyl ester/propanol derived method, and derivatization reagent uses 3- picoline and methylene chloride Respectively instead of the highly toxic pyridine and chloroform in Phenomenex EZ:Faast derivatization reagent, spreading out after optimization Biochemical reagents effectively increase the extraction efficiency of derivatization efficiency and product, improve method sensitivity, and only 2.5 μ L samples of need are The quantitative analysis of achievable 48 kinds of amino acid (former method needs 100 μ L blood plasma).

Claimed one kind¹³C₃Label amino-acid ester compound preparation method, amino acid solution to be marked according to The secondary addition derivatization reagent A and derivatization reagent B mixing, it is mixed to add neutral salt/oversaturated neutral salt solution It is even, mixed solution is obtained, mixed solution is carried out with ethyl acetate extract liquor is obtained by extraction, extract liquor is diluted with ethyl acetate, To obtain the final product, amino acid mixed liquor to be marked,¹³C₃The volume ratio of propyl alcohol, 3- picoline, derivatization reagent B are as follows: 20~60:4~10: 1~4:4~12.

Preferably, in amino acid solution to be marked each amino-acid ester compound second concentration level of concentration need >=calibration curve The concentration of corresponding amino acid in other calibration object.

Preferably, amino acid solution to be marked,¹³C₃Propyl alcohol, 3- picoline, derivatization reagent B volume ratio be 200: 31:10:30.

Due in LC-MS/MS method, there are matrix effect, i.e., the analyte of same concentrations different substrates (such as water, blood, Urine) in, the response (i.e. peak area or peak height) arrived using LC-MS/MS analysis detection is different, it is generally the case that matrix is water Sample, analyte response can be significantly hotter than the sample that matrix is blood plasma.If calibration object is prepared with water, and test sample is blood Slurry, then be unable to the concentration of analyte in actual response blood plasma.To offset matrix effect, and sample similar with analyte structure need to be added For the compound being not present again in product as internal standard, the analyte of isotope labelling is exactly best internal standard.In addition, internal standard solution makes With that can also offset imbibition in sample pretreatment process, pipetting and instrument inhale the inaccuracy that sample process generates.

Therefore, the internal standard solution combination of claimed a kind of derivatization HPLC-MS/MS method detection amino acid content, Including internal standard solution A and internal standard solution B；

Internal standard solution A is D₃Methionine and D₄The aqueous solution of cystathionie, D₃The concentration of methionine is 2~10 μm of ol/L, D₄- The concentration of cystathionie is 0.5~2 μm of ol/L；

Internal standard solution B is amino acid¹³C₃The amino-acid ester compound of label, the amino acid are glycine, alanine, β- Alanine, sarcosine, butyrine, γ-aminobutyric acid, B-AIB, serine, proline, valine, Soviet Union's ammonia Acid, hydroxyproline, leucine, isoleucine, 1-Methyl histidine, 3-Methyl histidine, phenylalanine, citrulling, asparagus fern ammonia Acid, glutamic acid, arginine, alpha-Aminoadipic acid, ornithine, histidine, lysine, tyrosine, homo-arginine, homotype melon ammonia Acid, reduced glutathione, homocysteine, cysteine, pyroglutamic acid, pipemidic acid and δ-amino oxopentanoic acid one kind or It is several.

When detection, the type for the amino acid that internal standard solution B is added is selected according to the type of amino acid to be detected, in above 44 When internal standard solution B is all added in amino acid, can be used for the detection of following 48 kinds of amino acid: amino acid be ethanol amine, pyroglutamic acid, L-Glycylglycine, 3-Methyl histidine, 5- hydroxylysine, 1-Methyl histidine, glycine, asparagine, Beta-alanine, Alanine, γ-aminobutyric acid, sarcosine, B-AIB, butyrine, methionine, γ-carboxyglutamic acid, group ammonia Acid, saccharopin, aspartic acid, glutamic acid, alpha-Aminoadipic acid, pipemidic acid, arginyl succinic acid, cystathionie, kynurenin, Arginine, homo-arginine, glutamine, citrulling, serine, homotype citrulling, hydroxyproline, N- acetyl group asparagus fern ammonia Acid, threonine, N- glycyl proline, δ-amino oxopentanoic acid, proline, ornithine, lysine, valine, tryptophan, reduction Type glutathione, phenylalanine, leucine, isoleucine, cysteine, homocysteine and tyrosine；Remove certain ammonia After base acid absolute quantification analysis can not be carried out to the amino acid in sample accordingly；In addition, removing pyroglutamic acid may will affect The accuracy of pyroglutamic acid and ethanol amine measurement in sample, remove homotype citrulling may will affect in sample homotype citrulling and δ-hydroxylysine measurement accuracy.

The combination of this internal standard solution can be reserved at least a year with long-term preservation at 4 DEG C.

The present invention significantly reduces analysis cost by synthesis stable isotope internal standard, the amino for avoiding purchase expensive Sour isotopic label, especially partial amino-acid temporarily without the isotopic label of commercial use, are realized to the exhausted of amino acid To quantitative analysis.Simultaneously as synthesis¹³C₃The cystathionie and methionine carboxylate of label are unstable in ethyl acetate, It is unfavorable for long term storage use, therefore this patent has used internal standard solution A as supplement, with realization to the exhausted of cystathionie and methionine To quantitative analysis (D₃Methionine and D₄Cystathionie is consistent with analyte structure respectively)；In addition, ethanol amine and 5- oxylysine Reagent isotope cannot be derivatized¹³C₃Propyl alcohol label, therefore both amino acid use the other ammonia close with its retention time Base acid isotope quantifies that (ethanol amine is used as internal standard¹³C₃The pyroglutamic acid of label is internal standard, and δ-hydroxylysine uses¹³C₃ The homotype citrulling of label is internal standard).

Preferably, D in internal standard solution A₃The concentration of methionine is 5 μm of ol/L.

Preferably, D in internal standard solution A₄The concentration of cystathionie is 1 μm of ol/L.

Preferably, amino acid¹³C₃The amino-acid ester compound preparation method of label uses approach described above.

For the difference of specific detection sample, the concentration of each amino acid according to amino acid each in sample to be tested concentration range And it adjusts.

For when test sample is blood plasma, the concentration of corresponding amino acid to be marked is provided, the present invention is not offered as It can be only used to detection blood plasma (or blood) sample, be not offered as the amino acid that the present invention only protects concentration.

Preferably, when sample to be tested is blood plasma, amino acid concentration to be marked is respectively as follows: 100~1000 μ of pyroglutamic acid Mol/L, 20~200 μm of ol/L of L-Glycylglycine, 20~200 μm of ol/L of 3-Methyl histidine, 1-Methyl histidine 20~ 20~200 μm of 200 μm of ol/L, 200~2000 μm of ol/L of glycine, 500~5000 μm of ol/L of asparagine, Beta-alanine ol/ L, 200~20004 μm of ol/L of alanine, 10~100 μm of ol/L of γ-aminobutyric acid, sarcosine 50~500 μm of ol/L, beta-aminos 20~200 μm of ol/L of isobutyric acid, 20~200 μm of ol/L of butyrine, 10-100 μm of ol/L of γ-carboxyglutamic acid, histidine 50~500 μm of 50~500 μm of ol/L, 10~100 μm of ol/L of saccharopin, 20~200 μm of ol/L of aspartic acid, glutamic acid ol/ L, 20~200 μm of ol/L of alpha-Aminoadipic acid, 20~200 μm of ol/L of pipemidic acid, arginyl succinic acid 10~100 μm of ol/L, dogs 20~200 μm of ol/L of urinary ammonia acid, 100~1000 μm of ol/L of arginine, 25~250 μm of ol/L of homo-arginine, glutamine 200 ~2000 μm of ol/L, 100~1000 μm of ol/L of citrulling, 100-1000 μm of ol/L of serine, 25~250 μ of homotype citrulling Mol/L, 40~400 μm of hydroxyproline, 25~250 μm of ol/L, N- Acetyl aspartate ol/L, 100~1000 μ of threonine 20~200 μm of ol/L, δ-amino oxopentanoic acids of mol/L, N- glycyl proline, 20~200 μm of ol/L, 100~1000 μ of proline Mol/L, 50~500 μm of ol/L of ornithine, 100~1000 μm of ol/L of lysine, valine 100~1000 μm of ol/L, tryptophans 50~500 μm of ol/L, 50~500 μm of ol/L of reduced glutathione, 100~1000 μm of ol/L of phenylalanine, leucine 100~ 1000 μm of ol/L, 100~1000 μm of ol/L of isoleucine, 100~1000 μm of ol/L of cysteine, homocysteine 100~ 0~500 μm of 1000 μm of ol/L, tyrosinase 15 ol/L.

It is highly preferred that amino acid concentration to be marked is respectively as follows: 100.0 μm of ol/ of pyroglutamic acid when sample to be tested is blood plasma L, 20.0 μm of ol/L of L-Glycylglycine, 20.0 μm of ol/L of 3-Methyl histidine, 20.0 μm of ol/L of 1-Methyl histidine, sweet ammonia Acid 200.0 μm of ol/L, 50.0 μm of ol/L of asparagine, 20.0 μm of ol/L of Beta-alanine, 200.0 μm of ol/L of alanine, gamma-amino 10.0 μm of ol/L of butyric acid, 50.0 μm of ol/L of sarcosine, 20.0 μm of ol/L of B-AIB, 20.0 μm of ol/L of butyrine, 10.0 μm of ol/L of γ-carboxyglutamic acid, 50.0 μm of ol/L of histidine, 10.0 μm of ol/L of saccharopin, 20.0 μm of ol/ of aspartic acid L, 50.0 μm of ol/L of glutamic acid, 20.0 μm of ol/L of alpha-Aminoadipic acid, pipemidic acid 20.0 μm of ol/L, 10.0 μ of arginyl succinic acid Mol/L, 20.0 μm of ol/L of kynurenin, 100.0 μm of ol/L of arginine, 25.0 μm of ol/L of homo-arginine, glutamine 200.0 μm ol/L, 100.1 μm of ol/L of citrulling, 100.0 μm of ol/L of serine, homotype citrulling 5.0 μm of ol/L, 40.0 μ of hydroxyproline 25.0 μm of ol/L of mol/L, N- Acetyl aspartate, 100.0 μm of ol/L of threonine, 20.0 μm of ol/L of N- glycyl proline, δ -20.0 μm of amino oxopentanoic acid ol/L, 100.0 μm of ol/L of proline, 50.0 μm of ol/L of ornithine, 100.0 μm of ol/L of lysine, 100.0 μm of ol/L of valine, 50.0 μm of ol/L of tryptophan, reduced glutathione 70.0 μm of ol/L, 100.0 μ of phenylalanine Mol/L, 100.0 μm of ol/L of leucine, 100.0 μm of ol/L of isoleucine, 75.0 μm of ol/L of cysteine, homocysteine 60.0 μm of ol/L, 0.0 μm of ol/L of tyrosinase 15.

Further, a kind of quantitative analysis of claimed derivatization HPLC-MS/MS method detects amino acid content Sample-pretreating method, comprising the following steps:

S1. sample is sequentially added into the internal standard solution A and three hydroxypropyl phosphine solution with water diluent, hybrid reaction obtains Mixed solution 1；

S2. mixed solution 1, the derivatization reagent A and the derivatization reagent B mixed rapidly, performed the derivatization instead It answers, obtains mixed solution 2；

S3. neutral salt/oversaturated neutral salt solution and any description above internal standard solution B is added to mixed solution 2, obtains Mixed solution 3；

S4. mixed solution 3, dry extract liquor is extracted with ethyl acetate；

S5. methanol water mixed liquid redissolves the extract liquor after drying, filter membrane；

Wherein, the concentration of three hydroxypropyl phosphines is not less than 0.5mg/mL in mixed solution 1；Sample diluting liquid, any of the above institute State internal standard solution A, the derivatization reagent A, the derivatization reagent B and any description above internal standard solution B volume ratio be 25~ 50:10~20:20~40:12~25:25~50,

The volume ratio of first alcohol and water is 4~5:6~5 in methanol water mixed liquid；

The volume of methanol water mixed liquid is 2~4 times of sample diluting liquid volume.

Three hydroxypropyl phosphines are added in the present invention in the sample, can be by cystine, homocystine and oxidized form of glutathione point It is not reduced to cysteine, homocysteine and reduced glutathione, the sulfur-containing amino acid that can also be incorporated on albumen Dissociation, thus measure total cysteine more stable in sample, more meaningful to medical diagnosis on disease, total homocysteine and The content of total reduced glutathione.

Preferably, sample to be tested is that blood plasma or urine dilute 20 times.

Preferably, sample to be tested is that cerebrospinal fluid dilutes 10 times.

Preferably, in step S1, reaction > 30s.

Preferably, in step S2, rapid mixing > 3s.

Preferably, in step S2, reaction > 1min.

Most preferably, in step S2,3min is reacted.

Preferably, the volume ratio of sample diluting liquid, internal standard solution A, derivatization reagent A, derivatization reagent B and internal standard solution B are 5:1:4:2.5:5

Preferably, the volume ratio of first alcohol and water is 2:3 in methanol water mixed liquid.

Preferably, the volume of methanol water mixed liquid is 2 times of sample diluting liquid volume；

Preferably, neutral salt is one of sodium chloride, potassium chloride, sodium sulphate.

It is highly preferred that neutral salt is sodium chloride.

The addition of neutral salt can enhance the ionic strength of water phase, accelerate two-phase laminated flow, improve extraction efficiency.

A kind of method of the quantitative analysis detection amino acid content of derivatization HPLC-MS/MS method, is carried out using the method Sample pre-treatments.

Preferably, liquid chromatogram uses C18 reverse-phase chromatographic column, and column temperature is 25~50 DEG C.

It is highly preferred that chromatographic column is Agilent ZorbaxElipseAAA chromatographic column.

Preferably, the specification of reversed C18 chromatographic column be 150 × 3.0mm i.d., 3.0 μm.

Preferably, column temperature is 35 DEG C

Preferably, mobile phase A is 1~10mmol/L formic acid aqueous ammonium；

Mobile phase B be acetonitrile, first alcohol and water mixed liquor, in terms of volume, Mobile phase B contain 50~75% acetonitrile, 40~20% methanol and 5~10% water；

Flow rate of mobile phase is 0.3~0.5mL/min；

Gradient elution program are as follows: 0~1.0min, 40% Mobile phase B, 1.0~7.0min Mobile phase B are gradually increased to 60%, 7.0~16.0min Mobile phase B are gradually increased to 70%, and it is 100% that 18.0~20min Mobile phase B is constant, 20.1~ Constant 22min Mobile phase B is 40%；

2~20 μ L of sampling volume.

It is highly preferred that mobile phase A is 5mmol/L formic acid aqueous ammonium.

It is highly preferred that Mobile phase B is acetonitrile, methanol and the water mixed liquid that volume ratio is 70:25:5.

It is highly preferred that flow rate of mobile phase is 0.4mL/min.

It is highly preferred that 10 μ L of sampling volume.

It is highly preferred that carrying out data acquisition using liquid chromatogram connection Q TRAP tandem mass spectrum.

It is highly preferred that Q TRAP tandem mass spectrum ionizes sample using positive ionization electrospray mode, preset mostly anti- Monitoring pattern is answered to carry out data acquisition to sample.

Tandem mass spectrum (MS/MS) acquisition parameter is shown in Table 1.

Table 1:

It is highly preferred that test sample is blood plasma, serum, cerebrospinal fluid, urine, tissue homogenate, soil extract or environment Water sample.

A kind of kit of derivatization HPLC-MS/MS method detection amino acid content, including derivatization reagent combination, The standard items of the internal standard solution combination and amino acid to be measured.

Preferably, the standard items of amino acid to be measured, including standard items 1, standard items 2 and standard items 3, wherein standard items 1 Are as follows: ethanol amine, glycine, alanine, Beta-alanine, sarcosine, butyrine, γ-aminobutyric acid, B-AIB, Serine, proline, valine, threonine, hydroxyproline, leucine, isoleucine, methionine, 1-Methyl histidine, 3- Methylhistidin, phenylalanine, citrulling, aspartic acid, glutamic acid, arginine, alpha-Aminoadipic acid, cystathionie, ornithine, Histidine, lysine, tyrosine, homo-arginine, homotype citrulling, 5- oxylysine, reduced glutathione, homotype half Cystine, cysteine, pyroglutamic acid, pipemidic acid, δ-amino oxopentanoic acid, γ-carboxyglutamic acid, kynurenin, saccharopin With one or more of standard solutions of arginyl succinic acid.

Standard items 2 are as follows: asparagine, N- acetyl aspartate, glycylglycine, glutamine and glycyl proline One or more of standard solution.

Standard items 3 are as follows: the standard solution of tryptophan.

Standard items 1 be 42 kinds in an acidic solution the stable amino acid of chemical property, Most amino-acids be dissolved in water, it is a small number of Macromolecular amino acid, which must be added to acid, to be dissolved, this is solution-stabilized, sets -80 DEG C of refrigerators and freezes, and can stablize preservation at least 3 years.

Standard items 2 are 5 kinds of amino acid unstable in acid solution, therefore are individually prepared with pure water, set -80 DEG C of refrigerators and freeze It deposits, can stablize at least 3 years.

Standard items 3 are tryptophan, when being mixed due to tryptophan with other amino acid, unstable chemcial property, therefore individually match System, sets -80 DEG C of refrigerators and freezes, and can stablize preservation at least 3 years.

The following contents also belongs to protection scope of the present invention:

The derivatization reagent combination, the combination of the preparation method, the internal standard solution, the sample-pretreating method, institute State method or any application in detection amino acid of the kit；

Derivatization reagent combination, the preparation method or internal standard solution combination is any is preparing derivatization HPLC- MS/MS method detects the application in the kit of amino acid content.

Preferably, it is ethanol amine, pyroglutamic acid, L-Glycylglycine, 3- methyl groups ammonia that the amino acid, which is amino acid, Acid, 5- hydroxylysine, 1-Methyl histidine, glycine, asparagine, Beta-alanine, alanine, γ-aminobutyric acid, flesh ammonia Acid, B-AIB, butyrine, methionine, γ-carboxyglutamic acid, histidine, saccharopin, aspartic acid, paddy Propylhomoserin, alpha-Aminoadipic acid, pipemidic acid, arginyl succinic acid, cystathionie, kynurenin, arginine, homo-arginine, paddy ammonia Amide, citrulling, serine, homotype citrulling, hydroxyproline, N- Acetyl aspartate, threonine, N- glycyl dried meat ammonia It is acid, δ-amino oxopentanoic acid, proline, ornithine, lysine, valine, tryptophan, reduced glutathione, phenylalanine, bright One or more of propylhomoserin, isoleucine, cysteine, homocysteine and tyrosine.

Compared with prior art, the invention has the following beneficial effects:

1, analysis speed is fast: 22min (see Fig. 1) has only been used in 48 kinds of amino acid quantitative analyses, substantially reduces analysis time, Improve detection flux；

2, specificity is high: each analyte is monitored by two pairs of ion pairs, improves method specificity, in addition efficient liquid Phase disengagement method realizes baseline separation to isomer, eliminates isomerism bring interference (see Fig. 4)；

3, each amino acid has an Isotopic Internal Standard to be demarcated, and improves the accuracy and repeatability of method；

4, high sensitivity: method lower limit of quantitation only needs 2.5 μ L blood plasma that 48 kinds of amino acid can be completed down to 0.01 μm of ol/L Quantitative analysis

The method of the invention analyzes the amino acid levels of plasma sample, using chloroformyl propyl ester/propanol derived reagent Pre-treatment is carried out to sample, the formula of derivatization reagent is optimized, improves derivatization efficiency, reduce reagent toxicity；This law Since required sample size is few, therefore sample can directly perform the derivatization reaction after water dilutes, and sample treatment is simple, quickly, repeat Property is good；This law is not required to extract amino acid using the Solid Phase Extraction that Phenomenex EZ:Faast is required, and is also not required to using chemistry examination Removing protein is removed in agent, operates more simple；Using HPLC-MS/MS method, obtain 48 kinds of amino acid (including 5 groups of isomers) all To being effectively maintained and separate；The mass spectrometry parameters of each amino acid are optimized, have obtained response height, specifically Property strong ion pair carry out data acquisition；Using chloroformyl propyl ester/¹³C₃Propyl alcohol has synthesized the isotope labelling of 44 kinds of amino acid Object is as internal standard, in addition the internal standard solution A of supplement, realizes the absolute quantification analysis of 48 kinds of amino acid.

This method can carry out fast quantitative analysis to 48 kinds of amino acid, and Monitoring lower-cut reaches 0.01 μm of ol/L, accuracy 89.9~113.4%, within-run and between-run analysis coefficient between 0.8%~7.7% and 2.6%~14.5%, reaches respectively What authoritative organization's (such as U.S. FDA) was recommended in the world establishes the index reached needed for bioanalytical method.

Derivatization reagent derivatization proposed by the present invention is high-efficient, and reagent toxicity is low；Sample size needed for method is few, processing is simple Single, quick, reproducible, testing cost is low；It realizes and has only used 22min to the absolute quantification analysis of 48 kinds of amino acid, significantly Analysis time is shortened, detection flux is improved；With very big application value.

Detailed description of the invention

Fig. 1 is 48 kinds of amino acid LC-MS/MS figures；Each peak corresponds to an amino acid, and the curve of each color is corresponding Each amino acid in corresponding table 1 is numbered at one ion pair, each peak.

Fig. 2 is children's amino acid spectrogram (A) without Aminoacidopathy and suffers from newborn's cholestasis syndrome Children's amino acid spectrogram (B)；Methionine in patients blood plasma, citrulling and lysine content are significantly raised, meet newborn's gallbladder Juice siltation syndrome patients' Plasma Amine Acid changes.

Fig. 3 is the chromatogram of reference examples 1；Threonine (Thr) and serine (Ser) contain amino, carboxyl and hydroxyl, spread out When biochemical reagents B dosage is 50 μ L, lead to threonine, 2 derivatization products occurs respectively in serine, and corresponding hydroxyl is not by amide Change (1) and hydroxyl is amidated the product of (2).

Fig. 4 is five groups of isomers with acetonitrile (A), methanol (B) or acetonitrile/methanol/water (70/25/5, v:v:v) It (C) is the chromatogram of Mobile phase B, mobile phase A is 5mmol/L formic acid aqueous ammonium.β-Ala, Beta-alanine；Ala, the third ammonia Acid；Sar, sarcosine；GABA, γ-aminobutyric acid；AIB, B-AIB；ABU, butyrine；The strong dried meat ammonia of Hyp, 4- Acid；N-Asp, N- acetyl aspartate；Leu, leucine；Ileu, isoleucine；3Me-His, 3-Methyl histidine；1Me- His, 1-Methyl histidine.

Specific embodiment

The present invention is made with specific embodiment with reference to the accompanying drawings of the specification and further being elaborated, the embodiment It is served only for explaining the present invention, be not intended to limit the scope of the present invention.Test method as used in the following examples is such as without spy Different explanation, is conventional method；Used material, reagent etc., unless otherwise specified, for the reagent commercially obtained And material.

Following embodiment is not offered as technical solution of the present invention and is suitable only for blood plasma for detecting plasma sample The detection of sample.

A kind of method of the 48 kinds of amino acid of fast quantitative analysis of embodiment 1

One, reagent

Pipemidic acid (purity >=97.0%) and butyrine (purity >=99.0%) standard items are purchased from An Pu company (Shanghai, China), remaining amino acid standard and three hydroxypropyl phosphines, ammonium formate be purchased from Sigma-Aldrich (St.Louis, MO,USA).Chloroformyl propyl ester, 3- picoline, isooctane and normal propyl alcohol are purchased from Tokyo Chemical Industry Co. (Tokyo,Japan).¹³C₃Propyl alcohol, D₃Methionine and D₄Cystathionie is purchased from Cambridge Isotope Laboratories (Andover,MA,USA)。

Solution is prepared

Amino acid standard reserving solution: accurately weighing each amino acid standard, is dissolved with ultrapure water or 1mol/L HCl solution Constant volume.

Standard items 1: according to the form below 2 accurately weighs amino acid standard, and water or 1mol/L aqueous hydrochloric acid solution 1mL dissolution is added, Allot single mark stock solution.According to the form below accurately takes single mark stock solution of certain volume to 25mL volumetric flask, adds water to scale constant volume, adds Water is set -80 DEG C of refrigerators and is frozen, can stablize preservation at least 3 years to scale constant volume.

Table 2:

Wherein, ethanol amine standard items are liquid, and density is 25 DEG C of 1.012g/mL at (concentration 828.5mmol/L), directly It connects and 30.2 μ l of ethanol amine standard items is pipetted with liquid-transfering gun mixes constant volume with other amino acid standard liquids to 25mL volumetric flask.

Standard items 2: accurately weighing amino acid standard by table 3, and water 1mL dissolution is added, and allots single mark stock solution.According to the form below It accurately takes single mark stock solution of certain volume to 25mL volumetric flask, adds water to scale constant volume, set -80 DEG C of refrigerators and freeze, can stablize At least 3 years.

Table 3:

Standard items 3: taking tryptophan standards product 25.3mg, and the dissolution of 1mL water is added, and is made into the storage that concentration is 123.82mmol/L Standby liquid.Take 202 μ l of tryptophan stock solution to add water to scale constant volume, set -80 DEG C of refrigerators and freeze to 25mL volumetric flask, can stablize to It is 3 years few.

Calibration object: it is quantitative for amino acid in unknown sample, need to prepare > 3 concentration rank calibration objects (it is recommended that 5-7 Concentration rank).Now by taking 7 concentration rank calibration objects as an example, calibration object: the same day is finished after preparing.

Calibration object 7: standard items 1, standard items 2, standard items 3 plus water mixing, volume ratio 1:1:1:7 are taken；

Calibration object 6: taking calibration object 7, dilutes 2 times with water；

Calibration object 2: taking calibration object 7, dilutes 100 times with water；

Calibration object 5: the 100 μ l of μ l calibration object 6 and 100 calibration objects 2 are taken to mix；

Calibration object 4: the 100 μ l of μ l calibration object 5 and 100 calibration objects 2 are taken to mix；

Calibration object 3: the 100 μ l of μ l calibration object 4 and 100 calibration objects 2 are taken to mix；

Calibration object 1: taking calibration object 2, dilutes 5 times with water.

Each amino acid alignment product concentration is shown in Table 4.

Table 4:

Three hydroxypropyl phosphine aqueous solutions: a certain amount of three hydroxypropyls phosphine is weighed, is dissolved in water and is settled to 0.5mg/mL.

Derivatization reagent A: normal propyl alcohol/3- picoline (77/23, v:v).

Derivatization reagent B: methylene chloride/chloroformyl propyl ester/isooctane (71.6/17.4/11, v:v:v).

Internal standard solution A: a certain amount of D is weighed respectively₃Methionine and D₄Cystathionie is dissolved in water, and is made into stock solution.It takes certain Volume D₃Methionine and D₄The mixing of cystathionie stock solution, is diluted with water, is made into containing D₃Methionine and D₄Cystathionine concentration difference For the use liquid of 5 μm of ol/L and 1 μm of ol/L；

Internal standard solution B: taking 200 μ l of amino acid mixed solution, and 31 μ l are added¹³C₃Propyl alcohol, 10 μ l 3- picolines mixing, adds Enter 30 μ l derivatization reagent B, rapid whirlpool mixing 1min, after excess chlorination sodium is added, 400 μ l of ethyl acetate is extracted 5 times, is merged Extract liquor adds ethyl acetate constant volume to 25mL volumetric flask.After packing, -20 DEG C are frozen,

The wherein concentration of the various amino acid of amino acid mixed solution are as follows: 100.0 μm of ol/L of pyroglutamic acid, the sweet ammonia of N- glycyl Acid 20.0 μm of ol/L, 20.0 μm of ol/L of 3-Methyl histidine, 20.0 μm of ol/L of 1-Methyl histidine, 200.0 μm of ol/L of glycine, 50.0 μm of ol/L of asparagine, 20.0 μm of ol/L of Beta-alanine, 200.0 μm of ol/L of alanine, 10.0 μm of ol/ of γ-aminobutyric acid L, 50.0 μm of ol/L of sarcosine, 20.0 μm of ol/L of B-AIB, 20.0 μm of ol/L of butyrine, γ-carboxyglutamic acid 10.0 μm of ol/L, 50.0 μm of ol/L of histidine, 10.0 μm of ol/L of saccharopin, aspartic acid 20.0 μm of ol/L, 50.0 μ of glutamic acid Mol/L, 20.0 μm of ol/L of alpha-Aminoadipic acid, 20.0 μm of ol/L of pipemidic acid, 10.0 μm of ol/L of arginyl succinic acid, kynurenin 20.0 μm of ol/L, 100.0 μm of ol/L of arginine, 25.0 μm of ol/L of homo-arginine, 200.0 μm of ol/L of glutamine, citrulling 100.1 μm of ol/L, 100.0 μm of ol/L of serine, 5.0 μm of ol/L of homotype citrulling, 40.0 μm of ol/L of hydroxyproline, N- acetyl group 25.0 μm of ol/L of aspartic acid, 100.0 μm of ol/L of threonine, N- glycyl proline 20.0 μm of ol/L, δ-amino oxopentanoic acid 20.0 μm of ol/L, 100.0 μm of ol/L of proline, 50.0 μm of ol/L of ornithine, lysine 100.0 μm of ol/L, 100.0 μ of valine Mol/L, 50.0 μm of ol/L of tryptophan, 70.0 μm of ol/L of reduced glutathione, 100.0 μm of ol/L of phenylalanine, leucine 100.0 μm of ol/L, 100.0 μm of ol/L of isoleucine, 75.0 μm of ol/L of cysteine, 60.0 μm of ol/L of homocysteine, junket 50.0 μm of ol/L of propylhomoserin.

Two, sample pre-treatments

It takes 2.5 μ l of blood plasma into 1mL flat bottom glass pipe, after adding 47.5 μ l of water to dilute, sequentially adds 10 μ l internal standards and use liquid A And 10 μ l, tri- hydroxypropyl phosphine solution, mixing place 3~5min, sequentially add derivatization reagent A40 μ l and derivatization reagent B 25 μ l mixes rapidly 3s, after standing 3min, sequentially adds 50 μ l saturated sodium chloride solutions, 50 μ l internal standards use liquid B and 150 μ l second Acetoacetic ester, whirlpool mixing, centrifugation 2min (2000rpm) promote two-phase laminated flow, take supernatant into 96 orifice plates, then with 200 μ l second Acetoacetic ester extraction is primary, and combining extraction liquid is dried with nitrogen, and 100 μ l methanol-water mixtures (2:3, v:v) are added and redissolve, mistake 0.2um GHP filter membrane (AcroPrep^TM96-well filterplate, Pall, USA), it is analyzed to instrument.

Three, sample data acquires

AB is connected using Shimadzu Ultrahigh Pressure Nexera liquid phase systems (Kyoto, Japan) Sciex3200Q TRAP tandem mass spectrometer (Foster City, CA, USA) carries out data acquisition.

Using Agilent Zorbax Elipse AAA chromatographic column (150 × 3.0mm i.d., 3.0 μm) to derivatization after Amino acid carries out chromatography, and column oven temperature setting is 35 DEG C.

Mobile phase includes A:5mmol/L formic acid aqueous ammonium；B: acetonitrile/methanol/water mixed liquid (70:25:5, v/v/v).

Flow rate of mobile phase is 0.4mL/min, gradient elution program are as follows: 0~1.0min, 40% Mobile phase B, the 1.0th~ 7.0min Mobile phase B is gradually increased to 60%, and 7.0~16.0min Mobile phase B is gradually increased to 70%, 18.0~20min Mobile phase B Constant is 100%, and constant 20.1~22min Mobile phase B is 40%.

10 μ L of sampling volume.

Mass spectrum ionizes sample using positive ionization electrospray mode, presets multiple-reaction monitoring pattern (scheduled Multiple reaction monitoring) data acquisition is carried out to analyte.Each analyte includes two pairs of MRM ions It is right, it is used for qualitative and quantitative analysis, each internal standard to be monitored by a pair of of MRM ion pair.The detection parameters of each MRM ion pair are shown in Table 5。

Table 5:48 kind amino acid name and mass spectrum acquisition parameter (each eluting peak in number corresponding diagram 1)

* DP, removes cluster voltage, and EP injects voltage；CE impact energy；MRM₁, multiple-reaction monitoring quota ion pair；MRM₂, mostly instead Qualitative ion pair should be monitored；IS, Isotopic Internal Standard monitor ion pair.

Four, testing result

48 kinds of amino acid LC-MS/MS testing results are shown in Fig. 1, and amino acid can be detected in 48.Each appearance time is not An ion pair of monitoring is represented with the peak of color, 3 ion pairs of each compound test, are quota ion pair respectively, qualitative Ion pair and internal standard ion pair (being shown in Table 2:48 kind amino acid name and mass spectrum acquisition parameter).Since multiple compounds are in same a period of time Between appearance and different compound response there is height to have low (see left ordinate), for going out for each compound of displaying for being more clear Peak time, therefore spectrogram is divided into two secondary displayings.

A kind of kit of derivatization HPLC-MS/MS method detection amino acid content of embodiment 2

One, it forms

Derivatization reagent combination, internal standard solution combination and standard items

Wherein, derivatization reagent combines are as follows: the derivatization reagent A and derivatization reagent B prepared in embodiment 1；

Internal standard solution combination are as follows: the internal standard solution A and internal standard solution B prepared in embodiment 1；

Standard items are as follows: standard items 1, standard items 2 and standard items 3.

Two, application method

(1) calibration object is prepared

1~calibration object of calibration object 7 is prepared according to the method for embodiment 1.

(2) sample pre-treatments

With embodiment 1.

(3) sample data acquires

With embodiment 1.

Embodiment 4 detects clinical sample

One, sample is detected

Choose the blood plasma of the volunteer without Aminoacidopathy and the patient with newborn's cholestasis syndrome into Row detection.

Two, detection method

As described in Example 1.

Three, testing result

Testing result is as shown in Figure 2.Methionine in patients blood plasma with newborn's cholestasis syndrome, citrulling Significantly raised with lysine content, volunteer's Plasma Amine Acid of no Aminoacidopathy is without exception.

The detection of 5 quality-control sample of embodiment

One, sample is detected

The precision of this law and accuracy pass through test ERNDIM (European Research Network for Evaluation and Improvement of Screening,Diagnosis,and Treatment of Inherited Disorders ofMetabolism, www.erndim.org) provide quality-control sample detected.

This law has detected 3 quality-control products (no.201701,201702,201703) of ERNDIM offer, and concentration contains Basic, normal, high three concentration ranks.

Two, detection method

As described in Example 1.This law measures value and median (target value) progress of value is measured in 250, whole world laboratory Comparison, calculates method accuracy ((this law measures the median that value is measured in value/250 laboratory) * 100%).By same Same quality-control product is tested 6 times in one batch and continuous 12 batches tests quality-control product, appraisal procedure withinrun precision and batch between Precision (variation within batch coefficient and interassay coefficient of variation).

Three, testing result

Include 26 kinds of amino acid in the quality-control product that ERNDIM is provided, removes taurine and cystine not in this law detection range Interior, remaining 24 kinds of amino acid can quantify (table 6), within-run and between-run analysis coefficient respectively 0.8%~7.7% and 2.6%~ Between 14.5%.In addition to homocysteine and isoleucine, remaining amino acid accuracy in detection 89.9~113.4% it Between.This law is measured total homocysteine rather than free cysteine, therefore is measured since three hydroxypropyl phosphines being added in the sample It is worth with other laboratory evaluations than higher, in addition, this law can not carry out completely optical isomer isoleucine and alloisoleucine Separation, therefore measure the summation that value is isoleucine and alloisoleucine.

Table 6:

Reference examples 1

One, sample is detected

Amino acid alignment product.

Two, detection method

As described in Example 1.Only the dosage of derivatization reagent B is changed to as 50 μ L.

Three, testing result

Testing result is as shown in Figure 3.In chromatogram, since threonine (Thr) and serine (Ser) contain amino, carboxylic Base and hydroxyl lead to threonine when derivatization reagent B dosage is 50 μ L, and 2 derivatization products occurs respectively in serine, corresponding Hydroxyl is not amidated (1) and hydroxyl is amidated the product of (2).

Reference examples 2

One, sample is detected

Amino acid alignment product.

Two, detection method

As described in Example 1.Formic acid ammonium concentration in mobile phase A is only changed to 10mmol/L.

Three, testing result

The results show that most amino acid response declines in chromatogram, reduce detection sensitivity.

Reference examples 3

One, sample is detected

Amino acid alignment product.

Two, detection method

As described in Example 1.Formic acid ammonium concentration in mobile phase A is only changed to 1mmol/L.

Three, testing result

The results show that the peak types such as 3-Methyl histidine are deteriorated in chromatogram, it is unfavorable for the quantitative detection of amino acid.

Reference examples 4

One, sample is detected

Amino acid alignment product.

Two, detection method

As described in Example 1, only Mobile phase B is changed to using pure acetonitrile.

Three, testing result

Testing result is as shown in Figure 4 A.4A group isomer can be obtained baseline separation in chromatogram, but leucine/different bright Propylhomoserin cannot achieve baseline separation, be unfavorable for the quantitative detection of amino acid.

Reference examples 5

One, sample is detected

Amino acid alignment product.

Two, detection method

As described in Example 1, Mobile phase B is only changed to pure methanol.

Three, testing result

Testing result is as shown in Figure 4 B.Hydroxyproline and N- acetyl aspartate is caused to flow out altogether using pure methanol, it is unfavorable In the quantitative detection of amino acid.

Claims (10)

1. a kind of derivatization reagent of HPLC-MS/MS method analysis detection amino acid combines, which is characterized in that tried including derivatization Agent A and derivatization reagent B；Wherein, derivatization reagent A is the mixed solution of normal propyl alcohol and 3- picoline, and derivatization reagent B is Methylene chloride, chloroformyl propyl ester and isooctane mixed solution.

2. a kind of¹³C₃The preparation method of the amino-acid ester compound of label, which is characterized in that amino acid solution to be marked successively adds Enter the mixing of derivatization reagent B described in derivatization reagent A and claim 1 described in claim 1, adds neutral salt/supersaturation Neutral salt solution mix, obtain mixed solution, be obtained by extraction extract liquor to mixed solution with ethyl acetate, extract liquor with Ethyl acetate dilution to get internal standard solution B, amino acid mixed liquor,¹³C₃The volume ratio of propyl alcohol, 3- picoline, derivatization reagent B Are as follows: 20~60:4~10:1~4:4~12.

3. a kind of internal standard solution combination of derivatization HPLC-MS/MS method detection amino acid content, which is characterized in that including internal standard solution A With internal standard solution B；

Internal standard solution A is D₃Methionine and D₄The aqueous solution of cystathionie, D₃The concentration of methionine is 2~10 μm of ol/L, D₄Guang sulphur The concentration of ether is 0.5~2 μm of ol/L；

Internal standard solution B is amino acid¹³C₃The amino-acid ester compound of label, the amino acid are glycine, alanine, β-the third ammonia Acid, sarcosine, butyrine, γ-aminobutyric acid, B-AIB, serine, proline, valine, threonine, hydroxyl Proline, leucine, isoleucine, 1-Methyl histidine, 3-Methyl histidine, phenylalanine, citrulling, aspartic acid, paddy Propylhomoserin, alpha-Aminoadipic acid, ornithine, histidine, lysine, tyrosine, homo-arginine, homotype citrulling, is gone back arginine Prototype glutathione, homocysteine, cysteine, pyroglutamic acid, pipemidic acid and δ-amino oxopentanoic acid one or more, Preferably, it is prepared using claim 2 the method¹³C₃The amino-acid ester compound of label.

4. a kind of method of the sample pre-treatments of derivatization HPLC-MS/MS quantitative analysis amino acid content, which is characterized in that The following steps are included:

S1. by sample with water diluent, internal standard solution A described in claim 2 and three hydroxypropyl phosphine solution are sequentially added, mixing is anti- It answers, obtains mixed solution 1；

S2. derivatization reagent B described in derivatization reagent A described in mixed solution 1, claim 1 and claim 1 is mixed rapidly It closes, performs the derivatization reaction, obtain mixed solution 2；

S3. internal standard solution B described in neutral salt/oversaturated neutral salt solution and claim 2 is added to mixed solution 2, is mixed Close solution 3；

S4. mixed solution 3, dry extract liquor is extracted with ethyl acetate；

S5. methanol water mixed liquid redissolves the extract liquor after drying, filter membrane；

Wherein, the concentration of three hydroxypropyl phosphines is not less than 0.5mg/mL in mixed solution 1；

Derivatization reagent A described in internal standard solution A, claim 1 described in sample diluting liquid, claim 2, spread out described in claim 1 The volume ratio of internal standard solution B described in biochemical reagents B and claim 2 is 25~50:10~20:20~40:12~25:25~50； The volume ratio of first alcohol and water is 4~5:6~5 in methanol water mixed liquid；

The volume of methanol water mixed liquid is 2~4 times of sample diluting liquid volume.

5. a kind of method of the analysis detection amino acid of derivatization HPLC-MS/MS method, which is characterized in that use claim 4 institute It states method and carries out sample pre-treatments.

6. method according to claim 4, which is characterized in that liquid chromatogram uses reversed C18 chromatographic column, and column temperature is 25~50 DEG C, the specification of reversed C18 chromatographic column is 150 × 3.0mm i.d., 3.0 μm；

Preferably, mobile phase A is 1~10mmol/L formic acid aqueous ammonium；

Mobile phase B be acetonitrile, first alcohol and water mixed liquor, in terms of volume, Mobile phase B contain 50~75% acetonitrile, 40~ 20% methanol and 5~10% water；

Flow rate of mobile phase is 0.3~0.5mL/min；

Gradient elution program are as follows: 0~1.0min, 40% Mobile phase B, the 1.0th~7.0 min Mobile phase B are gradually increased to 60%, 7.0 ~16.0 min Mobile phase Bs are gradually increased to 70%, and constant 18.0~20 min Mobile phase Bs are 100%, 20.1~22.0 min stream Dynamic phase B constant is 40%；

2~20 μ L of sampling volume.

7. according to claim 6 the method, which is characterized in that the acquisition parameter of ethanol amine: multiple-reaction monitoring quota ion pair 1 for 148.1/ 62.1 (m/z), go 36 V of cluster voltage, inject 6 V of voltage, 16 V of impact energy, multiple-reaction monitoring quota ion pair 2 For 148.1/ 62.1 (m/z), 36 V of cluster voltage, injection 6 V of voltage, 15 V of impact energy are removed, Isotopic Internal Standard monitors ion pair 175.2/84.1 (m/z), go 35 V of cluster voltage, inject 7 V of voltage, 6 V of impact energy；

The acquisition parameter of pyroglutamic acid: multiple-reaction monitoring quota ion pair 1 be 172.2/84.1 (m/z), go 35 V of cluster voltage, Inject 7 V of voltage, 24 V of impact energy, multiple-reaction monitoring quota ion pair 2 be 172.2/130.1 (m/z), go 40 V of cluster voltage, 6 V of voltage, 17 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 175.2/84.1 (m/z) removes 35 V of cluster voltage, injects 7 V of voltage, 24 V of impact energy；

Arginic acquisition parameter: multiple-reaction monitoring quota ion pair 1 is 303.2/70.1 (m/z), removes 54 V of cluster voltage, penetrates Enter 7 V of voltage, 57 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 303.2/156.2 (m/z), removes 50 V of cluster voltage, penetrates Enter 5 V of voltage, 30 V of impact energy, Isotopic Internal Standard monitoring ion pair 306.3/70.1 (m/z) removes 54 V of cluster voltage, injects electricity Press 7 V, 57 V of impact energy；

The acquisition parameter of homo-arginine: multiple-reaction monitoring quota ion pair 1 is 317.2/84.1 (m/z), removes cluster voltage 56 V, 4 V of voltage, 50 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 317.2/126.1 (m/z), removes cluster voltage 56 V, 4 V of voltage, 35 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 320.3/84.1 (m/z) removes 56 V of cluster voltage, penetrates Enter 4 V of voltage, 50 V of impact energy；

The acquisition parameter of glutamine: multiple-reaction monitoring quota ion pair 1 be 275.2/172.2 (m/z), go 38 V of cluster voltage, Inject 4 V of voltage, 20 V of impact energy, multiple-reaction monitoring quota ion pair 2 be 275.2/84.1 (m/z), go 38 V of cluster voltage, 5 V of voltage, 30 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 278.3/175.2 (m/z) removes 38 V of cluster voltage, penetrates Enter 4 V of voltage, 20 V of impact energy；

The acquisition parameter of citrulling: multiple-reaction monitoring quota ion pair 1 is 304.2/156.1 (m/z), removes 33 V of cluster voltage, penetrates Enter 10 V of voltage, 24 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 304.2/287.2 (m/z), go 32 V of cluster voltage, 11 V of voltage, 14 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 307.3/156.1 (m/z) removes 33 V of cluster voltage, penetrates Enter 10 V of voltage, 24 V of impact energy；

The acquisition parameter of glycylglycine: multiple-reaction monitoring quota ion pair 1 is 261.2/102.0 (m/z), removes cluster voltage 40 V, 5 V of voltage, 21 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 261.2/144.1 (m/z), removes cluster voltage 40 V, 5 V of voltage, 21 V of impact energy are injected, cluster voltage 40 is removed in Isotopic Internal Standard monitoring ion pair 264.3/102.0 (m/z) V, 6 V of voltage, 16 V of impact energy are injected；

The acquisition parameter of 3- methyl-histidine: multiple-reaction monitoring quota ion pair 1 is 298.2/96.1 (m/z), removes cluster voltage 50 V, 4 V of voltage, 50 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 298.2/256.2 (m/z), removes cluster voltage 50 V, 5 V of voltage, 27 V of impact energy are injected, cluster voltage 50 is removed in Isotopic Internal Standard monitoring ion pair 301.3/96.1 (m/z) V, 4 V of voltage, 50 V of impact energy are injected；

The acquisition parameter of serine: multiple-reaction monitoring quota ion pair 1 is 234.2/146.1 (m/z), removes 37 V of cluster voltage, penetrates Enter 6 V of voltage, 17 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 234.2/174.1 (m/z), removes 35 V of cluster voltage, penetrates Enter 5 V of voltage, 14 V of impact energy, Isotopic Internal Standard monitoring ion pair 237.2/146.1 (m/z) removes 37 V of cluster voltage, injects 6 V of voltage, 17 V of impact energy；

The acquisition parameter of homotype citrulling: multiple-reaction monitoring quota ion pair 1 is 318.2/170.2 (m/z), removes cluster voltage 41 V, 4 V of voltage, 25 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 318.2/127.1 (m/z), removes cluster voltage 41 V, inject 3 V of voltage, 30 V of impact energy, Isotopic Internal Standard monitoring ion pair 321.3/170.2 (m/z), go 41 V of cluster voltage, Inject 4 V of voltage, 25 V of impact energy；

The acquisition parameter of hydroxyproline: multiple-reaction monitoring quota ion pair 1 be 260.2/172.2 (m/z), go 40 V of cluster voltage, Inject 5 V of voltage, 19 V of impact energy, multiple-reaction monitoring quota ion pair 2 be 260.2/86.1 (m/z), go 38 V of cluster voltage, 6 V of voltage, 32 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 263.3/172.1 (m/z) removes 40 V of cluster voltage, penetrates Enter 5 V of voltage, 19 V of impact energy；

The acquisition parameter of 1- methyl-histidine: multiple-reaction monitoring quota ion pair 1 is 298.2/124.1 (m/z), removes cluster voltage 50 V, 4 V of voltage, 38 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 298.2/210.1 (m/z), removes cluster voltage 50 V, 4 V of voltage, 25 V of impact energy are injected, cluster voltage 50 is removed in Isotopic Internal Standard monitoring ion pair 301.2/124.1 (m/z) V, 4 V of voltage, 38 V of impact energy are injected；

δ-hydroxylysine acquisition parameter: multiple-reaction monitoring quota ion pair 1 is 334.2/125.1 (m/z), removes cluster voltage 48 V, 4 V of voltage, 39 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 334.2/125.1 (m/z), removes cluster voltage 48 V, inject 4 V of voltage, 61 V of impact energy, Isotopic Internal Standard monitoring ion pair 321.3/170.2 (m/z), go 41 V of cluster voltage, Inject 4 V of voltage, 25 V of impact energy；

The acquisition parameter of N- acetyl aspartate: multiple-reaction monitoring quota ion pair 1 is 260.2/130.1 (m/z), goes cluster electric It presses 40 V, inject 4 V of voltage, 25 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 260.2/172.2 (m/z), goes cluster electric It presses 40 V, inject 4 V of voltage, 25 V of impact energy, cluster voltage is removed in Isotopic Internal Standard monitoring ion pair 260.2/172.2 (m/z) 37 V, 5 V of voltage, 18 V of impact energy are injected；

The acquisition parameter of threonine: multiple-reaction monitoring quota ion pair 1 is 248.2/74.1 (m/z), removes 39 V of cluster voltage, penetrates Enter 6 V of voltage, 31 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 248.2/160.2 (m/z), removes 39 V of cluster voltage, penetrates Enter 5 V of voltage, 16 V of impact energy, Isotopic Internal Standard monitoring ion pair 251.3/74.1 (m/z) removes 39 V of cluster voltage, injects electricity Press 6 V, 31 V of impact energy；

The acquisition parameter of glycyl proline: multiple-reaction monitoring quota ion pair 1 is 301.2/158.2 (m/z), removes cluster voltage 42 V, 3.5 V of voltage, 21 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 301.2/70.0 (m/z), goes cluster electric It presses 45 V, inject 4.5 V of voltage, 45 V of impact energy, Isotopic Internal Standard monitoring ion pair 304.3/161.2 (m/z) goes cluster electric It presses 42 V, inject 3.5 V of voltage, 21 V of impact energy；

The acquisition parameter of glycine: multiple-reaction monitoring quota ion pair 1 is 204.2/102.0 (m/z), removes 45 V of cluster voltage, penetrates Enter 6 V of voltage, 16 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 204.2/118.1 (m/z), removes 45 V of cluster voltage, penetrates Enter 6 V of voltage, 16 V of impact energy, Isotopic Internal Standard monitoring ion pair 207.2/102.0 (m/z) removes 45 V of cluster voltage, injects 6 V of voltage, 16 V of impact energy；

The acquisition parameter of asparagine: multiple-reaction monitoring quota ion pair 1 be 243.2/157.1 (m/z), go 45 V of cluster voltage, 3.5 V of voltage, 14 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 243.2/115.0 (m/z), removes cluster voltage 44 V, 3.5 V of voltage, 19 V of impact energy are injected, cluster voltage 45 is removed in Isotopic Internal Standard monitoring ion pair 246.2/160.2 (m/z) V, 3.5 V of voltage, 14 V of impact energy are injected；

δ-amino oxopentanoic acid acquisition parameter: multiple-reaction monitoring quota ion pair 1 is 260.2/158.1 (m/z), removes cluster voltage 41 V, 5 V of voltage, 18 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 260.2/200.1 (m/z), removes cluster voltage 41 V, 6 V of voltage, 12 V of impact energy are injected, cluster voltage 41 is removed in Isotopic Internal Standard monitoring ion pair 263.3/158.1 (m/z) V, 5 V of voltage, 18 V of impact energy are injected；

The acquisition parameter of Beta-alanine: multiple-reaction monitoring quota ion pair 1 be 218.2/116.1 (m/z), go 39 V of cluster voltage, Inject 5 V of voltage, 19 V of impact energy, multiple-reaction monitoring quota ion pair 2 be 218.2/158.1 (m/z), go 39 V of cluster voltage, 6 V of voltage, 13 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 221.3/116.1 (m/z) removes 39 V of cluster voltage, penetrates Enter 5 V of voltage, 19 V of impact energy；

The acquisition parameter of alanine: multiple-reaction monitoring quota ion pair 1 is 218.2/130.1 (m/z), removes 40 V of cluster voltage, penetrates Enter 5 V of voltage, 17 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 218.2/158.1 (m/z), removes 39 V of cluster voltage, penetrates Enter 6 V of voltage, 13 V of impact energy, Isotopic Internal Standard monitoring ion pair 221.2/130.1 (m/z) removes 40 V of cluster voltage, injects 5 V of voltage, 17 V of impact energy；

The acquisition parameter of γ-aminobutyric acid: multiple-reaction monitoring quota ion pair 1 is 232.2/172.2 (m/z), removes cluster voltage 36 V, 4 V of voltage, 13 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 232.2/86.1 (m/z), removes cluster voltage 32 V, inject 5 V of voltage, 24 V of impact energy, Isotopic Internal Standard monitoring ion pair 235.3/172.1 (m/z), go 36 V of cluster voltage, Inject 4 V of voltage, 13 V of impact energy；

The acquisition parameter of sarcosine: multiple-reaction monitoring quota ion pair 1 is 218.2/116.0 (m/z), removes 37 V of cluster voltage, penetrates Enter 5 V of voltage, 16 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 218.2/88.1 (m/z), removes 37 V of cluster voltage, penetrates Enter 7 V of voltage, 24 V of impact energy, Isotopic Internal Standard monitoring ion pair 221.3/116.0 (m/z) removes 37 V of cluster voltage, injects 5 V of voltage, 16 V of impact energy；

The acquisition parameter of B-AIB: multiple-reaction monitoring quota ion pair 1 is 232.2/130.1 (m/z), removes cluster voltage 32 V, 5 V of voltage, 19 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 232.2/172.2 (m/z), removes cluster voltage 36 V, 5 V of voltage, 14 V of impact energy are injected, cluster voltage 32 is removed in Isotopic Internal Standard monitoring ion pair 235.3/130.1 (m/z) V, 5 V of voltage, 19 V of impact energy are injected；

The acquisition parameter of butyrine: multiple-reaction monitoring quota ion pair 1 is 232.2/144.1 (m/z), removes cluster voltage 30 V, 8 V of voltage, 16 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 232.2/172.1 (m/z), removes cluster voltage 30 V, inject 6 V of voltage, 11 V of impact energy, Isotopic Internal Standard monitoring ion pair 235.3/144.1 (m/z), go 30 V of cluster voltage, Inject 8 V of voltage, 16 V of impact energy；

The acquisition parameter of proline: multiple-reaction monitoring quota ion pair 1 is 244.2/156.1 (m/z), removes 42 V of cluster voltage, penetrates Enter 4 V of voltage, 18 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 244.2/70.1 (m/z), removes 38 V of cluster voltage, penetrates Enter 6 V of voltage, 40 V of impact energy, Isotopic Internal Standard monitoring ion pair 247.3/156.1 (m/z) removes 42 V of cluster voltage, injects 4 V of voltage, 18 V of impact energy；

The acquisition parameter of ornithine: multiple-reaction monitoring quota ion pair 1 is 347.2/287.2 (m/z), removes 47 V of cluster voltage, penetrates Enter 3.5 V of voltage, 14 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 347.2/156.1 (m/z), go 47 V of cluster voltage, Inject 3.5 V of voltage, 26 V of impact energy, Isotopic Internal Standard monitoring ion pair 350.3/290.2 (m/z), go 47 V of cluster voltage, Inject 3.5 V of voltage, 14 V of impact energy；

The acquisition parameter of methionine: multiple-reaction monitoring quota ion pair 1 be 278.1/190.2 (m/z), go 41 V of cluster voltage, 4.5 V of voltage, 17 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 278.1/142.1 (m/z), removes cluster voltage 41 V, 4.5 V of voltage, 23 V of impact energy are injected, cluster voltage 41 is removed in Isotopic Internal Standard monitoring ion pair 281.2/190.2 (m/z) V, 4.5 V of voltage, 17 V of impact energy are injected；

The acquisition parameter of histidine: multiple-reaction monitoring quota ion pair 1 is 370.2/196.2 (m/z), removes 40 V of cluster voltage, penetrates Enter 6 V of voltage, 27 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 370.2/284.2 (m/z), removes 48 V of cluster voltage, penetrates Enter 6 V of voltage, 23 V of impact energy, Isotopic Internal Standard monitoring ion pair 373.2/196.2 (m/z) removes 40 V of cluster voltage, injects 6 V of voltage, 27 V of impact energy；

The acquisition parameter of lysine: multiple-reaction monitoring quota ion pair 1 is 361.3/170.2 (m/z), removes 46 V of cluster voltage, penetrates Enter 6 V of voltage, 26 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 361.3/301.2 (m/z), removes 48 V of cluster voltage, penetrates Enter 6 V of voltage, 15 V of impact energy, Isotopic Internal Standard monitoring ion pair 364.4/170.1 (m/z) removes 46 V of cluster voltage, injects 6 V of voltage, 26 V of impact energy；

The acquisition parameter of saccharopin: multiple-reaction monitoring quota ion pair 1 be 429.3/369.2 (m/z), go 36 V of cluster voltage, Inject 8 V of voltage, 19 V of impact energy, multiple-reaction monitoring quota ion pair 2 be 429.3/170.2 (m/z), go 45 V of cluster voltage, 6 V of voltage, 29 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 435.4/375.4 (m/z) removes 36 V of cluster voltage, penetrates Enter 8 V of voltage, 19 V of impact energy；

The acquisition parameter of valine: multiple-reaction monitoring quota ion pair 1 is 246.3/158.2 (m/z), removes 35 V of cluster voltage, penetrates Enter 6 V of voltage, 15 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 246.3/116.1 (m/z), removes 35 V of cluster voltage, penetrates Enter 9 V of voltage, 25 V of impact energy, Isotopic Internal Standard monitoring ion pair 249.3/158.2 (m/z) removes 35 V of cluster voltage, injects 6 V of voltage, 15 V of impact energy；

The acquisition parameter of aspartic acid: multiple-reaction monitoring quota ion pair 1 be 304.3/216.2 (m/z), go 40 V of cluster voltage, Inject 5 V of voltage, 17 V of impact energy, multiple-reaction monitoring quota ion pair 2 be 304.3/130.1 (m/z), go 40 V of cluster voltage, 5 V of voltage, 27 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 310.4/219.2 (m/z) removes 40 V of cluster voltage, penetrates Enter 5 V of voltage, 17 V of impact energy；

The acquisition parameter of glutamic acid: multiple-reaction monitoring quota ion pair 1 is 318.2/172.2 (m/z), removes 44 V of cluster voltage, penetrates Enter 5.5 V of voltage, 21 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 318.2/84.1 (m/z), go 44 V of cluster voltage, 5 V of voltage, 38 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 324.3/175.2 (m/z) removes 44 V of cluster voltage, penetrates Enter 5.5 V of voltage, 21 V of impact energy；

The acquisition parameter of tryptophan: multiple-reaction monitoring quota ion pair 1 is 333.2/245.2 (m/z), removes 45 V of cluster voltage, penetrates Enter 5.5 V of voltage, 22 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 333.2/273.1 (m/z), go 45 V of cluster voltage, 6 V of voltage, 16 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 336.3/245.1 (m/z) removes 45 V of cluster voltage, penetrates Enter 5.5 V of voltage, 22 V of impact energy；

The acquisition parameter of γ-carboxyglutamic acid: multiple-reaction monitoring quota ion pair 1 is 344.2/258.2 (m/z), removes cluster voltage 42 V, 4.5 V of voltage, 15 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 344.2/170.1 (m/z), goes cluster electric It presses 42 V, inject 4.5 V of voltage, 26 V of impact energy, Isotopic Internal Standard monitoring ion pair 350.3/264.3 (m/z) goes cluster electric It presses 42 V, inject 4.5 V of voltage, 15 V of impact energy；

The acquisition parameter of total reduced glutathione: multiple-reaction monitoring quota ion pair 1 is 564.3/162.1 (m/z), goes 55 V of cluster voltage, 6 V of voltage, 30 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 564.3/447.2 (m/z), goes 55 V of cluster voltage, 7 V of voltage, 19 V of impact energy are injected, cluster is removed in Isotopic Internal Standard monitoring ion pair 570.4/162.1 (m/z) 55 V of voltage, 6 V of voltage, 30 V of impact energy are injected；

The acquisition parameter of alpha-Aminoadipic acid: multiple-reaction monitoring quota ion pair 1 is 332.2/98.1 (m/z), removes cluster voltage 35 V, 5.5 V of voltage, 37 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 332.2/244.2 (m/z), removes cluster voltage 35 V, 4 V of voltage, 18 V of impact energy are injected, cluster voltage 35 is removed in Isotopic Internal Standard monitoring ion pair 338.3/98.1 (m/z) V, 5.5 V of voltage, 37 V of impact energy are injected；

The acquisition parameter of phenylalanine: multiple-reaction monitoring quota ion pair 1 be 294.2/206.1 (m/z), go 50 V of cluster voltage, Inject 5 V of voltage, 17 V of impact energy, multiple-reaction monitoring quota ion pair 2 be 294.2/120.1 (m/z), go 50 V of cluster voltage, 4 V of voltage, 35 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 297.3/206.1 (m/z) removes 50 V of cluster voltage, penetrates Enter 5 V of voltage, 17 V of impact energy；

The acquisition parameter of leucine: multiple-reaction monitoring quota ion pair 1 is 260.2/172.2 (m/z), removes 45 V of cluster voltage, penetrates Enter 6.5 V of voltage, 18 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 260.2/86.1 (m/z), go 45 V of cluster voltage, 7 V of voltage, 28 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 263.3/172.2 (m/z) removes 45 V of cluster voltage, penetrates Enter 6.5 V of voltage, 18 V of impact energy；

The acquisition parameter of isoleucine: multiple-reaction monitoring quota ion pair 1 be 260.2/130.1 (m/z), go 40 V of cluster voltage, Inject 6 V of voltage, 24 V of impact energy, multiple-reaction monitoring quota ion pair 2 be 260.2/172.2 (m/z), go 43 V of cluster voltage, 5 V of voltage, 16 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 263.3/130.1 (m/z) removes 40 V of cluster voltage, penetrates Enter 6 V of voltage, 24 V of impact energy；

The acquisition parameter of pipemidic acid: multiple-reaction monitoring quota ion pair 1 is 258.3/128.1 (m/z), removes 38 V of cluster voltage, penetrates Enter 4 V of voltage, 29 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 258.3/170.2 (m/z), removes 38 V of cluster voltage, penetrates Enter 5 V of voltage, 19 V of impact energy, Isotopic Internal Standard monitoring ion pair 261.3/128.1 (m/z) removes 38 V of cluster voltage, injects 4 V of voltage, 29 V of impact energy；

The acquisition parameter of argininosuccinic acid: multiple-reaction monitoring quota ion pair 1 is 529.3/469.3 (m/z), removes cluster voltage 47 V, 6 V of voltage, 23 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 529.3/156.1 (m/z), removes cluster voltage 50 V, inject 6 V of voltage, 43 V of impact energy, Isotopic Internal Standard monitoring ion pair 535.5/475.4 (m/z), go 47 V of cluster voltage, Inject 6 V of voltage, 23 V of impact energy；

The acquisition parameter of total cysteine: multiple-reaction monitoring quota ion pair 1 is 336.2/190.1 (m/z), removes cluster voltage 42 V, 5.5 V of voltage, 18 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 336.2/248.2 (m/z), goes cluster electric It presses 48 V, inject 6 V of voltage, 17 V of impact energy, cluster voltage is removed in Isotopic Internal Standard monitoring ion pair 339.3/193.2 (m/z) 42 V, 5.5 V of voltage, 18 V of impact energy are injected；

The acquisition parameter of cystathionie: multiple-reaction monitoring quota ion pair 1 is 479.3/230.2 (m/z), removes 45 V of cluster voltage, penetrates Enter 7 V of voltage, 23 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 479.3/142.1 (m/z), removes 45 V of cluster voltage, penetrates Enter 7 V of voltage, 28 V of impact energy, Isotopic Internal Standard monitoring ion pair 485.4/233.3 (m/z) removes 45 V of cluster voltage, injects 7 V of voltage, 23 V of impact energy；

The acquisition parameter of total homocysteine: multiple-reaction monitoring quota ion pair 1 is 350.2/142.1 (m/z), removes cluster 46 V of voltage, 5.5 V of voltage, 24 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 350.2/204.1 (m/z), goes 46 V of cluster voltage, 5.5 V of voltage, 24 V of impact energy are injected, Isotopic Internal Standard monitoring ion pair 353.3/142.1 (m/z) is gone 48 V of cluster voltage, 5.5 V of voltage, 18 V of impact energy are injected；

The acquisition parameter of tyrosine: multiple-reaction monitoring quota ion pair 1 is 413.3/136.1 (m/z), removes 31 V of cluster voltage, penetrates Enter 3.5 V of voltage, 45 V of impact energy, multiple-reaction monitoring quota ion pair 2 is 413.3/222.1 (m/z), go 31 V of cluster voltage, Inject 3.5 V of voltage, 33 V of impact energy, Isotopic Internal Standard monitoring ion pair 416.3/136.1 (m/z), go 31 V of cluster voltage, Inject 3.5 V of voltage, 45 V of impact energy；

The acquisition parameter of kynurenin: multiple-reaction monitoring quota ion pair 1 be 423.2/146.1 (m/z), go 50 V of cluster voltage, 4.5 V of voltage, 42 V of impact energy are injected, multiple-reaction monitoring quota ion pair 2 is 423.2/260.1 (m/z), removes cluster voltage 50 V, 4.5 V of voltage, 21 V of impact energy are injected, cluster voltage 50 is removed in Isotopic Internal Standard monitoring ion pair 426.3/146.1 (m/z) V, 4.5 V of voltage, 42 V of impact energy are injected.

8. a kind of kit of derivatization HPLC-MS/MS method detection amino acid content, which is characterized in that including claim 1 institute State the standard items of internal standard solution combination and amino acid to be measured described in derivatization reagent combination, claim 3；Preferably, amino to be measured The standard items of acid, including standard items 1, standard items 2 and standard items 3, wherein standard items 1 are as follows: ethanol amine, glycine, alanine, Beta-alanine, sarcosine, butyrine, γ-aminobutyric acid, B-AIB, serine, proline, valine, Soviet Union's ammonia Acid, hydroxyproline, leucine, isoleucine, methionine, 1-Methyl histidine, 3-Methyl histidine, phenylalanine, melon ammonia It is acid, aspartic acid, glutamic acid, arginine, alpha-Aminoadipic acid, cystathionie, ornithine, histidine, lysine, tyrosine, same Type arginine, homotype citrulling, 5- oxylysine, reduced glutathione, homocysteine, cysteine, burnt paddy ammonia Acid, pipemidic acid, δ-amino oxopentanoic acid, γ-carboxyglutamic acid, kynurenin, saccharopin and arginyl succinic acid one kind or Several standard solutions；

Standard items 2 are as follows: in asparagine, N- acetyl aspartate, glycylglycine, glutamine and glycyl proline One or more of standard solutions；

Standard items 3 are as follows: the standard solution of tryptophan.

9. preparation method described in the combination of derivatization reagent described in claim 1, claim 2, internal standard solution group described in claim 3 Kit is any described in sample-pretreating method, claim 5 the method or claim 8 described in conjunction, claim 4 is examining Survey the application in amino acid.

10. internal standard solution described in preparation method described in the combination of derivatization reagent described in claim 1, claim 2 or claim 3 Combine any application in the kit kind for preparing derivatization HPLC-MS/MS method detection amino acid content.