CN110016554B - Method for bioleaching semiconductor sulfide minerals by using jarosite to enhance photocatalysis - Google Patents
Method for bioleaching semiconductor sulfide minerals by using jarosite to enhance photocatalysis Download PDFInfo
- Publication number
- CN110016554B CN110016554B CN201910369442.6A CN201910369442A CN110016554B CN 110016554 B CN110016554 B CN 110016554B CN 201910369442 A CN201910369442 A CN 201910369442A CN 110016554 B CN110016554 B CN 110016554B
- Authority
- CN
- China
- Prior art keywords
- jarosite
- bioleaching
- sulfide mineral
- semiconductor sulfide
- photocatalytic semiconductor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229910052935 jarosite Inorganic materials 0.000 title claims abstract description 104
- 229910052569 sulfide mineral Inorganic materials 0.000 title claims abstract description 68
- 239000004065 semiconductor Substances 0.000 title claims abstract description 58
- 230000001699 photocatalysis Effects 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 43
- 238000007146 photocatalysis Methods 0.000 title description 6
- 238000002386 leaching Methods 0.000 claims abstract description 57
- 241000894006 Bacteria Species 0.000 claims abstract description 19
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical compound [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 102000005298 Iron-Sulfur Proteins Human genes 0.000 claims abstract description 13
- 108010081409 Iron-Sulfur Proteins Proteins 0.000 claims abstract description 13
- 230000001590 oxidative effect Effects 0.000 claims abstract description 13
- 238000005728 strengthening Methods 0.000 claims abstract description 13
- 229910052951 chalcopyrite Inorganic materials 0.000 claims description 38
- DVRDHUBQLOKMHZ-UHFFFAOYSA-N chalcopyrite Chemical compound [S-2].[S-2].[Fe+2].[Cu+2] DVRDHUBQLOKMHZ-UHFFFAOYSA-N 0.000 claims description 38
- 239000010949 copper Substances 0.000 claims description 11
- 238000011081 inoculation Methods 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 8
- 238000001291 vacuum drying Methods 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 229910052683 pyrite Inorganic materials 0.000 claims description 6
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 claims description 6
- 239000011028 pyrite Substances 0.000 claims description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 229910052948 bornite Inorganic materials 0.000 claims description 2
- 229910052947 chalcocite Inorganic materials 0.000 claims description 2
- 239000002002 slurry Substances 0.000 claims 1
- 230000001851 biosynthetic effect Effects 0.000 abstract description 32
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 2
- 238000005286 illumination Methods 0.000 description 23
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 16
- 239000001963 growth medium Substances 0.000 description 15
- 241000605222 Acidithiobacillus ferrooxidans Species 0.000 description 14
- 229910001431 copper ion Inorganic materials 0.000 description 13
- 239000000243 solution Substances 0.000 description 13
- 230000003321 amplification Effects 0.000 description 10
- 238000003199 nucleic acid amplification method Methods 0.000 description 10
- -1 iron ions Chemical class 0.000 description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 8
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 8
- 229910052500 inorganic mineral Inorganic materials 0.000 description 8
- 239000011707 mineral Substances 0.000 description 8
- 229910052939 potassium sulfate Inorganic materials 0.000 description 8
- 239000008367 deionised water Substances 0.000 description 7
- 229910021641 deionized water Inorganic materials 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000001914 filtration Methods 0.000 description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 229910052742 iron Inorganic materials 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000012299 nitrogen atmosphere Substances 0.000 description 5
- 230000001133 acceleration Effects 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229910052603 melanterite Inorganic materials 0.000 description 4
- 239000002366 mineral element Substances 0.000 description 4
- 230000005855 radiation Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 239000000377 silicon dioxide Substances 0.000 description 4
- 235000012239 silicon dioxide Nutrition 0.000 description 4
- 229910052717 sulfur Inorganic materials 0.000 description 4
- 230000001360 synchronised effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 241000589902 Leptospira Species 0.000 description 2
- 241000589921 Leptospirillum ferrooxidans Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000033116 oxidation-reduction process Effects 0.000 description 2
- 238000002161 passivation Methods 0.000 description 2
- 241000266272 Acidithiobacillus Species 0.000 description 1
- 241001261719 Ferrithrix thermotolerans Species 0.000 description 1
- 241000589925 Leptospirillum Species 0.000 description 1
- 238000004833 X-ray photoelectron spectroscopy Methods 0.000 description 1
- 230000003570 biosynthesizing effect Effects 0.000 description 1
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Inorganic materials [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 229910052955 covellite Inorganic materials 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 238000005272 metallurgy Methods 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000009853 pyrometallurgy Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B15/00—Obtaining copper
- C22B15/0063—Hydrometallurgy
- C22B15/0065—Leaching or slurrying
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B3/00—Extraction of metal compounds from ores or concentrates by wet processes
- C22B3/18—Extraction of metal compounds from ores or concentrates by wet processes with the aid of microorganisms or enzymes, e.g. bacteria or algae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- Mechanical Engineering (AREA)
- Metallurgy (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Environmental & Geological Engineering (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geochemistry & Mineralogy (AREA)
- Geology (AREA)
- Manufacture And Refinement Of Metals (AREA)
Abstract
本发明公布了一种黄钾铁矾强化光催化半导体硫化矿物生物浸出的方法，属于生物冶金技术领域。在外加0.1‑6g/L生物合成黄钾铁矾以及光强为4000Lux‑12000Lux的可见光条件下利用嗜酸铁硫氧化细菌浸出半导体硫化矿物。显著提高半导体硫化矿物生物浸出效率。在可见光与黄钾铁矾联合作用下半导体硫化矿物浸出率比黑暗条件下不添加黄钾铁矾的对照组浸出率提高了33.5‑35.7％。本发明的方法能够显著提高半导体硫化矿物的浸出率，使得生物浸出技术在资源加工领域上的应用具有重大意义。The invention discloses a method for strengthening photocatalytic semiconductor sulfide mineral bioleaching by jarosite, which belongs to the technical field of biometallurgy. The semiconducting sulfide minerals were leached by acidophilic iron-sulfur oxidizing bacteria under the addition of 0.1-6g/L biosynthetic jarosite and visible light with a light intensity of 4000Lux-12000Lux. Significantly improve the bioleaching efficiency of semiconducting sulfide minerals. Under the combined action of visible light and jarosite, the leaching rate of semiconductor sulfide minerals increased by 33.5-35.7% compared with the control group without adding jarosite under dark conditions. The method of the invention can significantly improve the leaching rate of the semiconductor sulfide minerals, so that the application of the bioleaching technology in the field of resource processing is of great significance.
Description
Technical Field
The invention belongs to the technical field of biological metallurgy, and particularly relates to a method for bioleaching semiconductor sulfide minerals by using jarosite to enhance photocatalysis.
Background
At present, the copper is extracted mainly by adopting a pyrometallurgy method, and the method can generate a large amount of pollution and has high cost. The bioleaching technology has the advantages of simple operation, mild reaction conditions, environmental friendliness, low cost and the like, and can treat low-grade complex minerals which are difficult to treat, so that the bioleaching technology is widely concerned. At present, 20-25% of copper all over the world is obtained by bioleaching, so that bioleaching has good application prospect in the field of mineral resource processing. However, sulfide mineral bioleaching has the defects of long leaching period, low leaching rate and the like, and further wide application of the sulfide mineral bioleaching is limited. There is therefore a need to find a viable method to increase the bioleaching efficiency of sulphide minerals. In recent years, a large number of researchers have improved leaching of sulfide mineral bacteria by means of adjusting the pH value and oxidation-reduction potential of the system, adding a catalyst, mixing flora at high temperature during use, and the like. They have met with some success, but they have ignored the semiconducting properties of sulfide mineral bioleaching processes.
The photo-generated electrons excited when the visible light irradiates the semiconductor sulfide minerals can be converted into chemical energy utilized by acidophilic iron sulfur oxidizing bacteria to promote the growth of the bacteria, so that the biological leaching of the sulfide minerals can be promoted by utilizing the photocatalytic performance of the sulfide minerals. But more jarosite is generated in the process of bioleaching the sulfide minerals under the illumination condition, the jarosite can passivate the surfaces of the sulfide minerals, the transfer rate of photoproduction electrons can be inhibited, the recombination of the photoproduction electrons and photoproduction holes is increased, and the efficiency of bioleaching the photocatalytic sulfide minerals is reduced. Therefore, the inhibition of the formation of jarosite on the surface of the sulfide minerals has important significance for improving the bacterial leaching efficiency of the photocatalytic sulfide minerals. The added jarosite can be used as a seed crystal to promote the formation of jarosite on the surface of the seed crystal in a leaching system, so that the formation of passivation jarosite on the surface of a sulfide mineral can be reduced, and the biological leaching efficiency of the sulfide mineral is remarkably improved. The method remarkably increases the biological leaching efficiency of the photocatalytic semiconductor sulfide minerals by additionally biologically synthesizing the jarosite.
Disclosure of Invention
The invention aims to improve the biological leaching efficiency of photocatalytic semiconductor sulfide minerals, and provides a method for strengthening the biological leaching of photocatalytic semiconductor sulfide minerals by jarosite.
The purpose of the invention is realized by the following modes:
a method for leaching jarosite from semiconductor sulfide minerals by using photocatalysis is characterized in that jarosite is added in the process of leaching the semiconductor sulfide minerals by using photocatalysis.
According to the method for the jarosite-enhanced photocatalytic biological leaching of semiconductor sulfide minerals, the concentration of the jarosite in a photocatalytic biological leaching system of semiconductor sulfide minerals is 0.1-6 g/L; further preferably, the concentration is 0.5 to 2 g/L.
The jarosite is used for strengthening the photocatalytic biological leaching of semiconductor sulfide minerals, and the jarosite is biosynthesized.
The jarosite-enhanced photocatalytic semiconductor sulfide mineral bioleaching method comprises the following steps: according to 108-109cells/mL inoculum size acidophilic ferrooxidans were inoculated into 250mL containing 0.08-0.32M FeSO4·7H2O and 13.33-53.30mM K2SO4pH 2.0-3.0 in aqueous solution, and FeSO4·7H2O and K2SO4The molar ratio of (1: 6), culturing at 20-50 deg.C with the rotation speed of shaker of 100-. The yellow precipitate is collected, preferably with 0.1M H2SO4And repeatedly washing with deionized water for 3-5 times, and vacuum drying at 50 deg.C for 12 hr.
The method for strengthening the photo-catalytic biological leaching of the semiconductor sulfide minerals by the jarosite has the advantages that the illumination intensity in a photo-catalytic biological leaching system of the semiconductor sulfide minerals is 4000-12000lux, and is preferably 8000-12000 lux.
The method for the jarosite-enhanced photocatalytic biological leaching of semiconductor sulfide minerals is characterized in that 1-5% of semiconductor sulfide minerals with pulp concentration are added into a photocatalytic semiconductor sulfide mineral biological leaching system.
The biological leaching of the jarosite-enhanced photocatalytic semiconductor sulfide mineral is carried out by utilizing acidophilic iron-sulfur oxidizing bacteria, preferably domesticating the acidophilic iron-sulfur oxidizing bacteria.
The acidophilic iron-sulfur oxidizing bacteria include: acidithiobacillus ferrooxidans (Acidithiobacillus ferrooxidans), Acidithiobacillus mesophilic (Ferrithrixthermotolerans) or leptospirillum ferrooxidans (Leptospirillum ferrooxidans). All belong to conventional commercially available mineral leaching bacteria.
The method for leaching the semiconductor sulfide minerals by using jarosite to enhance photocatalysis comprises the following steps of inoculating the semiconductor sulfide minerals by using an inoculation amount of 1 multiplied by 107-9×107cells/ml acidophilic iron sulfur oxidizing bacteria are inoculated into a leaching system.
The method for the jarosite-enhanced photocatalytic semiconductor sulfide mineral bioleaching comprises the steps that the pH value of a leaching system is 1.5-3.0, the rotating speed of a shaking table is 100-200rpm, and the temperature is 20-50 ℃.
The jarosite-enhanced photocatalytic bioleaching method for semiconductor sulfide minerals comprises the following steps: one or more of chalcopyrite, covellite, bornite, chalcocite and pyrite, preferably the semiconductor sulphide mineral is crushed and sieved to a particle size below 74 μm, and stored in a nitrogen atmosphere for later use before use.
A method of biosynthesizing jarosite enhanced photocatalytic bioleaching of semiconductor sulphide minerals, preferably comprising the steps of:
(1) firstly, inoculating 5-10ml of acidophilic iron-sulfur oxidizing bacteria bacterial liquid into 100ml of 9K culture medium containing semiconductor sulfide ore with 1% ore pulp concentration, and repeatedly performing acclimatization culture until the bacterial concentration can reach 108cells/ml, finishing the first domestication, collecting bacteria, repeating the steps, and sequentially domesticating in the semiconductor sulfide ore pulp with the ore pulp concentration of 2%, 3%, 4% and 5% until the acidophilic iron-sulfur oxidizing bacteria can adapt to the semiconductor sulfide ore pulp concentration of 1-5% of the ore pulp concentration.
(2) According to 108-109cells/mL inoculum size acidophilic ferrooxidans were inoculated into 250mL containing 0.08-0.32M FeSO4·7H2O and 13.33-53.30mM K2SO4pH 2.0-3.0 in aqueous solution, and FeSO4·7H2O and K2SO4The molar ratio of (1 to 6) is 100-200rpm at 20-50 deg.C, collecting yellow precipitate after 3-7 days, and adding 0.1M H2SO4Repeatedly washing with deionized water for 3-5 times, and vacuum drying at 50 deg.C for 12 hrSo as to obtain the biosynthetic jarosite.
(3) Inoculating acidophilic iron-sulfur oxidizing bacteria with inoculum size of 3 × 107-9×107cells/ml are inoculated into a 9K culture medium containing semiconductor sulfide minerals with the concentration of 1-5% of ore pulp for amplification culture, and the conditions of amplification culture are that the initial pH is 1.5-3.0 and the temperature is 20-50 ℃.
(4) Centrifugally collecting the acidophilic iron-sulfur oxidizing bacteria cultured to the middle logarithmic phase in the step (3), wherein the inoculation amount of the acidophilic iron-sulfur oxidizing bacteria is 1 × 107-9×107cells/ml is inoculated into a 9k culture medium containing 0.1-6.0g/L biosynthetic jarosite and 1-5% pulp concentration semiconductor sulfide mineral, pH is 1.5-3.0, and leaching is carried out under the conditions that the illumination intensity is 4000-12000lux, the rotating speed of a shaking table is 100-200rpm and the temperature is 20-50 ℃.
(5) Respectively measuring pH and oxidation reduction potential in the solution of the bacterial leaching system by using a pH meter and a portable potential measuring instrument every 3 days, and measuring Fe in the solution by using an ICP emission spectrometer2+Total iron ions and Cu2+And (4) concentration.
(6) And after bioleaching is finished, filtering and collecting slag by using filter paper to analyze the surface appearance and the phase and element composition.
The 9K culture medium of the invention has the following formula: (NH)4)2SO43.0g/L、KC1 0.1g/L、K2HPO40.5g/L、MgSO4·7H2O0.5g/L、Ca(NO3)20.01g/L, with 0.01mol/L H2SO4Adjusting the pH value to 1.5-3.0.
The added biosynthetic jarosite can be used as a seed crystal to promote the jarosite in a leaching system to be mainly formed on the surface of the seed crystal, so that the formation of the jarosite on the surface of a semiconductor sulfide mineral is reduced. Surface morphology analysis, phase and element composition analysis show that jarosite can reduce passivation substances (jarosite and S)n 2-/S0) are formed on the surface of the sulphide minerals (as shown in fig. 6 and 7), so that the added biosynthetic jarosite promotes bioleaching of the sulphide minerals. Moreover, the invention unexpectedly finds that the effect of the biological synthesized jarosite is better than that of the chemical synthesized jarosite.
The method obviously improves the bacterial leaching efficiency of the photocatalytic semiconductor sulfide mineral by adding the biosynthetic jarosite, has simple required equipment, mild reaction condition, low cost and environmental protection, and thus provides possibility for wide application of the photocatalytic semiconductor sulfide mineral bioleaching. The invention is mainly suitable for semiconductor sulfide minerals.
Drawings
FIG. 1 is a graph showing the trend of copper ion concentration in a solution of the leaching system of example 1;
FIG. 2 is a graph showing the trend of the copper ion concentration in the solution of the leaching system of example 2;
FIG. 3 is a graph showing the trend of the copper ion concentration in the solution of the leaching system of example 3;
FIG. 4 is a graph showing the trend of the copper ion concentration in the solution of the leaching system of example 4;
FIG. 5 is a graph showing the trend of the copper ion concentration in the solution of the leaching system of example 5;
FIG. 6 is a scanning electron microscope image of surface topography;
dark 0.0g/L means no light and no addition of biosynthetic jarosite,
dark 1.0g/L indicates no light and 1.0g/L biosynthetic jarosite is added to the leaching system,
illumination of 0.0g/L indicates illumination without addition of biosynthetic jarosite,
illumination 1.0g/L indicates illumination and 1.0g/L of biosynthetic jarosite was added to the leaching system.
FIG. 7 is an elemental composition analysis X-ray photoelectron spectroscopy analysis chart;
dark 0.0g/L means no light and no addition of biosynthetic jarosite,
dark 1.0g/L indicates no light and 1.0g/L biosynthetic jarosite is added to the leaching system,
illumination of 0.0g/L indicates illumination without addition of biosynthetic jarosite,
illumination 1.0g/L indicates illumination and 1.0g/L of biosynthetic jarosite was added to the leaching system.
Detailed Description
The following specific examples or embodiments are intended to further illustrate the invention, but are not intended to limit the invention.
Example 1
The method of the embodiment is mainly carried out according to the following steps:
(1) according to 4X 108cells/mL inoculum size Cochlospira ferrooxidans was inoculated to 250mL of a solution containing 0.16MFeSO4·7H2O and 26.65mM K2SO4The culture was carried out at 40 ℃ with a shaker rotation speed of 170rpm in an aqueous solution of pH2.0, and after 3 days, the yellow precipitate was collected by filtration through filter paper and 0.1M of H was used2SO4Repeatedly cleaning with deionized water for 3-5 times, and vacuum drying at 50 deg.C for 12 hr to obtain jarosite material;
(2) crushing and screening a chalcopyrite ore sample until the particle size is below 74 mu m, storing the chalcopyrite ore sample for later use in a nitrogen atmosphere, detecting by synchronous radiation XRD before an experiment to find that the main phase composition of the chalcopyrite ore is chalcopyrite, and a small amount of silicon dioxide and pyrite exist, and analyzing mineral elements by XRF to be Cu, 33.21%, S, 31.59%, Fe, 28.21%, O, 4.88% and other elements to be 2.11%;
(3) pre-domesticated leptospirillum ferrixide with the inoculum size of 3.2 multiplied by 107Inoculating cells/ml into a 9K culture medium containing chalcopyrite with the pulp concentration of 2 percent for amplification culture, wherein the conditions of the amplification culture are that the initial pH is 2.0 and the temperature is 40 ℃;
(4) centrifugally collecting leptospira ferrooxidans cultured to the middle logarithmic phase in the step (3), wherein the inoculation amount of leptospira ferrooxidans is 3.2 multiplied by 107cells/ml are inoculated into 9k culture medium containing 0.5g/L biosynthetic jarosite and 2% pulp concentration chalcopyrite, pH2.0, and leached for 28 days under the conditions of illumination intensity of 12000lux, shaking table rotation speed of 170rpm and temperature of 40 ℃.
(5) Measuring Cu in the solution by adopting an ICP emission spectrometer every 3 days2+Concentration (shown in fig. 1).
And (4) conclusion: as shown in FIG. 1, the leaching rate of chalcopyrite under the combined action of 12000Lux visible light with light intensity and 0.5g/L biosynthetic jarosite is improved by 34 percent compared with the leaching rate of a control group without adding the biosynthetic jarosite under dark conditions, and is improved by 6.9 percent compared with the leaching rate of the chalcopyrite without adding the biosynthetic jarosite with light intensity of 12000 Lux. When no illumination is added and the jarosite is biosynthesized, the chalcopyrite is seriously passivated on the 16 th day, the concentration of copper ions is not increased any more, and the illumination or the biosynthesized jarosite copper ions can be continuously increased until the higher acceleration is achieved in 28 days.
Example 2
The method of the embodiment is mainly carried out according to the following steps:
(1) according to 5X 108Inoculation amount of cells/mL Acidithiobacillus ferrooxidans was inoculated into 250mL of a culture medium containing 0.16MFeSO4·7H2O and 26.65mM K2SO4The culture was carried out at 30 ℃ with a shaker rotation speed of 170rpm in an aqueous solution of pH2.0, and after 3 days, the yellow precipitate was collected by filtration through filter paper and 0.1M of H was used2SO4Repeatedly cleaning with deionized water for 3-5 times, and vacuum drying at 50 deg.C for 12 hr to obtain jarosite material;
(2) crushing and screening a chalcopyrite ore sample until the particle size is below 74 mu m, storing the chalcopyrite ore sample for later use in a nitrogen atmosphere, detecting by synchronous radiation XRD before an experiment to find that the main phase composition of the chalcopyrite ore is chalcopyrite, and a small amount of silicon dioxide and pyrite exist, and analyzing mineral elements by XRF to be Cu, 33.21%, S, 31.59%, Fe, 28.21%, O, 4.88% and other elements to be 2.11%;
(3) inoculating Acidithiobacillus ferrooxidans domesticated in advance to 3.2 × 107Inoculating cells/ml into a 9K culture medium containing chalcopyrite with the pulp concentration of 2 percent for amplification culture, wherein the conditions of the amplification culture are that the initial pH is 2.0 and the temperature is 30 ℃;
(4) centrifugally collecting the acidithiobacillus ferrooxidans cultured to the middle logarithmic phase in the step (3), wherein the inoculation amount of the acidithiobacillus ferrooxidans is 3.2 multiplied by 107cells/ml are inoculated into 9k culture medium containing 1.0g/L biosynthetic jarosite and 2% pulp concentration chalcopyrite, pH2.0, and leached for 28 days under the conditions of illumination intensity of 12000lux, shaking table rotation speed of 170rpm and temperature of 30 ℃.
(5) Every 3 days, the ICP emission spectrometer is used for measuring Cu in the solution2+Concentration (shown in fig. 2).
And (4) conclusion: as shown in FIG. 2, the leaching rate of chalcopyrite under the combined action of 12000Lux visible light with light intensity and 1.0g/L biosynthetic jarosite is increased by 35.7 percent compared with the leaching rate of a control group without adding the biosynthetic jarosite under dark conditions, and is increased by 8.2 percent compared with the leaching rate of the light intensity of 12000Lux biosynthetic jarosite without adding the biosynthetic jarosite. When no illumination is added and the jarosite is biosynthesized, the chalcopyrite is seriously passivated on the 16 th day, the concentration of copper ions is not increased any more, and the illumination or the biosynthesized jarosite copper ions can be continuously increased until the higher acceleration is achieved in 28 days.
Example 3
The method of the embodiment is mainly carried out according to the following steps:
(1) according to 4X 108Inoculation amount of cells/mL Acidithiobacillus ferrooxidans was inoculated into 250mL of a culture medium containing 0.16MFeSO4·7H2O and 26.65mM K2SO4The culture was carried out at 30 ℃ with a shaker rotation speed of 170rpm in an aqueous solution of pH2.0, and after 3 days, the yellow precipitate was collected by filtration through filter paper and 0.1M of H was used2SO4Repeatedly cleaning with deionized water for 3-5 times, and vacuum drying at 50 deg.C for 12 hr to obtain jarosite material;
(2) crushing and screening a chalcopyrite ore sample until the particle size is below 74 mu m, storing the chalcopyrite ore sample for later use in a nitrogen atmosphere, detecting by synchronous radiation XRD before an experiment to find that the main phase composition of the chalcopyrite ore is chalcopyrite, and a small amount of silicon dioxide and pyrite exist, and analyzing mineral elements by XRF to be Cu, 33.21%, S, 31.59%, Fe, 28.21%, O, 4.88% and other elements to be 2.11%;
(3) inoculating Acidithiobacillus ferrooxidans domesticated in advance to 3.2 × 107Inoculating cells/ml into a 9K culture medium containing chalcopyrite with the pulp concentration of 2 percent for amplification culture, wherein the conditions of the amplification culture are that the initial pH is 2.0 and the temperature is 30 ℃;
(4) centrifugally collecting the acidithiobacillus ferrooxidans cultured to the middle logarithmic phase in the step (3), wherein the inoculation amount of the acidithiobacillus ferrooxidans is 3.2 multiplied by 107cells/ml are inoculated into 2g/L biosynthetic jarosite and 2% pulp concentrationLeaching chalcopyrite with a concentration of 9k in a culture medium with a pH value of 2.0 for 28 days under the conditions that the illumination intensity is 12000lux, the rotating speed of a shaking table is 170rpm and the temperature is 30 ℃;
(5) every 3 days, the ICP emission spectrometer is used for measuring Cu in the solution2+Concentration (shown in fig. 3).
And (4) conclusion: as shown in FIG. 3, the leaching rate of chalcopyrite under the combined action of 12000Lux visible light with 2g/L biosynthetic jarosite is improved by 34.6 percent compared with the leaching rate of a control group without adding the biosynthetic jarosite under dark conditions, and is improved by 7.3 percent compared with the leaching rate of the chalcopyrite without adding the biosynthetic jarosite under 12000Lux visible light. When no illumination is added and the jarosite is biosynthesized, the chalcopyrite is seriously passivated on the 16 th day, the concentration of copper ions is not increased any more, and the illumination or the biosynthesized jarosite copper ions can be continuously increased until the higher acceleration is achieved in 28 days.
Example 4
The method of the embodiment is mainly carried out according to the following steps:
(1) according to 4X 108Inoculation amount of cells/mL Acidithiobacillus ferrooxidans was inoculated into 250mL of a culture medium containing 0.16MFeSO4·7H2O and 26.65mM K2SO4The culture was carried out at 30 ℃ with a shaker rotation speed of 170rpm in an aqueous solution of pH2.0, and after 3 days, the yellow precipitate was collected by filtration through filter paper and 0.1M of H was used2SO4Repeatedly cleaning with deionized water for 3-5 times, and vacuum drying at 50 deg.C for 12 hr to obtain jarosite material;
(2) crushing and screening a chalcopyrite ore sample until the particle size is below 74 mu m, storing the chalcopyrite ore sample for later use in a nitrogen atmosphere, detecting by synchronous radiation XRD before an experiment to find that the main phase composition of the chalcopyrite ore is chalcopyrite, and a small amount of silicon dioxide and pyrite exist, and analyzing mineral elements by XRF to be Cu, 33.21%, S, 31.59%, Fe, 28.21%, O, 4.88% and other elements to be 2.11%;
(3) inoculating Acidithiobacillus ferrooxidans domesticated in advance to 3.2 × 107Inoculating cells/ml into a 9K culture medium containing chalcopyrite with the pulp concentration of 2 percent for amplification culture, wherein the conditions of the amplification culture are that the initial pH is 2.0 and the temperature is 30 ℃;
(4) centrifugally collecting the acidithiobacillus ferrooxidans cultured to the middle logarithmic phase in the step (3), wherein the inoculation amount of the acidithiobacillus ferrooxidans is 3.2 multiplied by 107cells/ml are inoculated into 9k culture medium containing 4g/L of biosynthetic jarosite and 2% of chalcopyrite with pulp concentration and pH2.0, and are leached for 28 days under the conditions that the illumination intensity is 12000lux, the rotating speed of a shaking table is 170rpm and the temperature is 30 ℃;
(5) every 3 days, the ICP emission spectrometer is used for measuring Cu in the solution2+Concentration (shown in fig. 4).
And (4) conclusion: as shown in FIG. 4, the leaching rate of chalcopyrite under the combined action of 12000Lux visible light with 4g/L biosynthetic jarosite is improved by 33.5 percent compared with the leaching rate of a control group without adding the biosynthetic jarosite under dark conditions, and is improved by 6.5 percent compared with the leaching rate of the chalcopyrite without adding the biosynthetic jarosite under 12000Lux visible light with 4g/L biosynthetic jarosite. When no illumination is added and the jarosite is biosynthesized, the chalcopyrite is seriously passivated on the 16 th day, the concentration of copper ions is not increased any more, and the illumination or the biosynthesized jarosite copper ions can be continuously increased until the higher acceleration is achieved in 28 days.
Example 5
The procedure of this example is the same as example 2, but the jarosite used is chemically synthesized, not biosynthesized, and the jarosite chemical synthesis procedure is: 0.2g of KNO3And 1.18g of Fe2(SO4)3Adding into 100ml 0.1M sulfuric acid solution, magnetically stirring at 80 deg.C for 12 hr, filtering to collect yellow precipitate, and adding 0.1M H2SO4Repeatedly washing with deionized water for 3-5 times, and vacuum drying at 50 deg.C for 12 hr to obtain jarosite material.
The results show that: the leaching rate of chalcopyrite under the combined action of the visible light with the light intensity of 12000Lux and the chemically synthesized jarosite with the light intensity of 1g/L is improved by 26.8 percent compared with the leaching rate of a control group without adding the jarosite under the dark condition, and is increased by 1.1 percent compared with the leaching rate of the jarosite without adding the biologically synthesized jarosite with the light intensity of 12000Lux (as shown in figure 5). The promoting effect of the chemically synthesized jarosite is obviously weaker than that of the biologically synthesized jarosite.
Claims (12)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910369442.6A CN110016554B (en) | 2019-05-06 | 2019-05-06 | Method for bioleaching semiconductor sulfide minerals by using jarosite to enhance photocatalysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910369442.6A CN110016554B (en) | 2019-05-06 | 2019-05-06 | Method for bioleaching semiconductor sulfide minerals by using jarosite to enhance photocatalysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110016554A CN110016554A (en) | 2019-07-16 |
| CN110016554B true CN110016554B (en) | 2020-04-24 |
Family
ID=67193115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910369442.6A Active CN110016554B (en) | 2019-05-06 | 2019-05-06 | Method for bioleaching semiconductor sulfide minerals by using jarosite to enhance photocatalysis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110016554B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110629023A (en) * | 2019-10-17 | 2019-12-31 | 中南大学 | A multifunctional biometallurgical reactor |
| CN112080637B (en) * | 2020-08-24 | 2021-11-23 | 中国科学院微生物研究所 | Photoelectric energy method for promoting microbial Ar-4 biological metallurgy leaching rate |
| CN112375903B (en) * | 2020-11-12 | 2022-03-18 | 西安建筑科技大学 | Method for enhancing leaching of arsenic pyrite microorganisms |
| CN115287453A (en) * | 2022-06-29 | 2022-11-04 | 中南大学 | Method for enhancing chalcopyrite bioleaching by flotation collector |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7604783B2 (en) * | 2004-12-22 | 2009-10-20 | Placer Dome Technical Services Limited | Reduction of lime consumption when treating refractor gold ores or concentrates |
| CN102127657B (en) * | 2011-03-21 | 2012-12-19 | 中南大学 | Comprehensive recovery method for extracting ferrovanadium from stone coal acid immersion liquid |
| CN104726707A (en) * | 2013-12-23 | 2015-06-24 | 北京有色金属研究总院 | Iron balancing technology in low-grade sulfide ore biological heap leaching-leachate purification-extraction and separation process |
| CN103740763B (en) * | 2014-01-20 | 2015-09-30 | 陈朋 | The method of jarosite is prepared in a kind of microbial transformation |
| CN105886760B (en) * | 2016-05-11 | 2018-05-29 | 中南大学 | A kind of method that graphene promotes photocatalytic semiconductor sulfide mineral bioleaching |
| CN106929672B8 (en) * | 2017-04-07 | 2019-01-11 | 中南大学 | A method of using crystal seed induction except iron promotes chalcopyrite Bioleaching |
-
2019
- 2019-05-06 CN CN201910369442.6A patent/CN110016554B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110016554A (en) | 2019-07-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110016554B (en) | Method for bioleaching semiconductor sulfide minerals by using jarosite to enhance photocatalysis | |
| Zhao et al. | Comparison of bioleaching and dissolution process of p-type and n-type chalcopyrite | |
| Tian et al. | Effects of redox potential on chalcopyrite leaching: An overview | |
| Liu et al. | Utilization of electrochemical impedance spectroscopy for monitoring pyrite oxidation in the presence and absence of Acidithiobacillus ferrooxidans | |
| Xia et al. | Investigation of the sulfur speciation during chalcopyrite leaching by moderate thermophile Sulfobacillus thermosulfidooxidans | |
| Yang et al. | Photogenerated-hole scavenger for enhancing photocatalytic chalcopyrite bioleaching | |
| LIU et al. | Relatedness between catalytic effect of activated carbon and passivation phenomenon during chalcopyrite bioleaching by mixed thermophilic Archaea culture at 65° C | |
| Zhao et al. | Catalytic effect of visible light and Cd2+ on chalcopyrite bioleaching | |
| Santos et al. | Effect of redox potential on chalcopyrite dissolution imposed by addition of ferrous ions | |
| CN104561544B (en) | Light-intensified bioleaching method for semiconductor minerals | |
| Li et al. | Effect of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans on preg-robbing of gold by graphite from thiourea leaching solution | |
| Yavari et al. | Intensified bioleaching of copper from chalcopyrite: decoupling and optimization of the chemical stage | |
| Hong et al. | The use of biogenic Fe3+ and H2SO4 generated from pyrite waste to enhance bornite bioleaching: a potential utilization of acid mine drainage | |
| CN1827805A (en) | Treatment of Sulfide Ore with Thermoacidophilic Bacteria | |
| Feng et al. | Insights into the enhancement mechanism coupled with adapted adsorption behavior from mineralogical aspects in bioleaching of copper-bearing sulfide ore by Acidithiobacillus sp. | |
| Castro et al. | Improving zinc recovery by thermoacidophilic archaeon Acidianus copahuensis using tetrathionate | |
| Nie et al. | Differential utilization and transformation of sulfur allotropes, μ-S and α-S8, by moderate thermoacidophile Sulfobacillus thermosulfidooxidans | |
| ZENG et al. | Isolation and identification of moderately thermophilic acidophilic iron-oxidizing bacterium and its bioleaching characterization | |
| CN111321294B (en) | A method for directional regulation of indigenous microbial community to improve chalcopyrite leaching efficiency | |
| CN105886760B (en) | A kind of method that graphene promotes photocatalytic semiconductor sulfide mineral bioleaching | |
| Rehman et al. | Bioleaching of high grade Pb–Zn ore by mesophilic and moderately thermophilic iron and sulphur oxidizers | |
| CN110157906B (en) | Method for promoting bioleaching of semiconductor sulfide ore by cooperation of cadmium ions and visible light | |
| CN105648212B (en) | A kind of method that oxalic acid promotes photocatalytic semiconductor sulfide mineral bioleaching | |
| CN109022776B (en) | Method for enhancing leaching of bornite by using high-iron sphalerite | |
| Xia et al. | Sulfur speciation transformation during bioleaching of pyrite-containing sphalerite concentrate by thermophile Sulfolobus metallicus at 65 C |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |