CN110016488B - Method for separating lignin and co-producing xylo-oligosaccharide from lignocellulose alkaline oxidation pretreatment liquid - Google Patents
Method for separating lignin and co-producing xylo-oligosaccharide from lignocellulose alkaline oxidation pretreatment liquid Download PDFInfo
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- CN110016488B CN110016488B CN201910315108.2A CN201910315108A CN110016488B CN 110016488 B CN110016488 B CN 110016488B CN 201910315108 A CN201910315108 A CN 201910315108A CN 110016488 B CN110016488 B CN 110016488B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
- C07G1/00—Lignin; Lignin derivatives
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H6/00—Macromolecular compounds derived from lignin, e.g. tannins, humic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
Abstract
The invention discloses a method for separating lignin and co-producing xylo-oligosaccharide from lignocellulose alkaline oxidation pretreatment liquid, which adopts a low-cost gradient pH adjustment and green and environment-friendly enzyme hydrolysis combination mode, does not need organic solvents such as ethanol and the like, has small acid consumption, realizes the separation of lignin in the lignocellulose alkaline oxidation pretreatment liquid and the hydrolysis and conversion of xylanase into xylo-oligosaccharide, has low cost and is green and environment-friendly, and solves the problems of large ethanol consumption and high cost in the prior art that the separation and purification of lignin by an acid precipitation method need to consume a large amount of acid and xylan is separated by ethanol precipitation.
Description
The technical field is as follows:
the invention relates to a method for separating lignin and co-producing xylo-oligosaccharide from lignocellulose alkaline oxidation pretreatment liquid.
Background art:
the lignocellulose alkaline oxidation pretreatment liquid is a yellow liquid, and is a mixture of a liquid phase obtained by performing alkaline oxidation pretreatment on agricultural and forestry biomass such as bagasse, straws and corncobs and a washing waste liquid (see CN108588144A) generated in a solid residue washing process, wherein the liquid phase is obtained by performing solid-liquid separation on the agricultural and forestry biomass. The lignocellulose alkaline oxidation pretreatment liquid is alkaline, the pH value of the lignocellulose alkaline oxidation pretreatment liquid is 9-10, the lignocellulose alkaline oxidation pretreatment liquid is complex in components, rich in macromolecules such as xylan and lignin, and further comprises micromolecular eluates such as residual alkali, an oxidant, saccharides and acetic acid. The alkaline oxidation pretreatment solution for lignocellulose has poor biodegradability, and if the alkaline oxidation pretreatment solution is directly discharged without treatment, the environment pollution is easily caused. The lignin and xylan in the lignocellulose alkaline oxidation pretreatment liquid are recycled, so that the economic benefit of lignocellulose biorefinery can be improved, and the wastewater treatment difficulty can be reduced. The acid precipitation method and the ethanol precipitation method are common methods for separating lignin and xylan from the alkali-leached lignocellulose pretreatment solution. The acid precipitation method for separating and purifying lignin usually needs to adjust the pH value of the alkali pretreatment solution to about 2, needs to consume a large amount of acid, particularly for concentrated alkali extraction pretreatment solution, and the ethanol precipitation method for separating xylan has the problems of high ethanol consumption, high cost and the like, and is not beneficial to practical industrial production application.
The invention content is as follows:
the invention aims to provide a method for separating lignin and co-producing xylo-oligosaccharide from lignocellulose alkaline oxidation pretreatment liquid, which adopts a low-cost gradient pH adjustment and green and environment-friendly enzyme hydrolysis combination mode, does not need organic solvents such as ethanol and the like, has low acid consumption, realizes the separation of lignin in the lignocellulose alkaline oxidation pretreatment liquid and the hydrolysis and conversion of xylanase into xylo-oligosaccharide, has low cost and environmental protection, and solves the problems of large consumption of acid and high consumption of ethanol and high cost in the prior art that the separation and purification of lignin by an acid precipitation method need to consume a large amount of acid and the separation of xylan by ethanol precipitation.
The invention is realized by the following technical scheme:
the method for separating lignin and co-producing xylo-oligosaccharide from lignocellulose alkaline oxidation pretreatment solution comprises the following steps:
(1) performing solid-liquid separation on the lignocellulose alkaline oxidation pretreatment solution by adopting a centrifugal method to obtain supernatant 1;
(2) separation of water-insoluble xylan and production of xylo-oligosaccharide: adjusting the pH value of the supernatant 1 to 5-6, performing vacuum rotary evaporation concentration and solid-liquid separation to obtain a precipitate 1 and a supernatant 2, dispersing the precipitate 1 by using a buffer solution with the pH value of 4-5, adding 50-500 IU/g of endoxylanase, and hydrolyzing at 45-60 ℃ for 9-24 h to obtain a xylo-oligosaccharide solution 1;
(3) lignin separation and xylo-oligosaccharide production: continuously adjusting the pH value of the supernatant 2 obtained in the step (2) to 4-5, performing solid-liquid separation to obtain a precipitate 2 and a supernatant 3, dispersing the precipitate 2 by using a buffer solution with the pH value of 4-5, adding 50-500 IU/g of endoxylanase, hydrolyzing at 45-60 ℃ for 9-24 h, and performing solid-liquid separation to respectively obtain a xylo-oligosaccharide solution 2 and lignin 1;
(4) adding 50-500 IU/mL of endoxylanase into the supernatant 3 obtained in the step (3), hydrolyzing at 45-60 ℃ for 9-24 h, performing solid-liquid separation to obtain a precipitate 3 and a xylo-oligosaccharide solution 3, and washing the precipitate 3 with water to obtain lignin 2.
Placing the crushed and dried lignocellulose biomass into a glass reactor, adding 0.5-5% by mass of alkali liquor and 0.5-5% by mass of hydrogen peroxide, controlling the solid-liquid mass ratio to be 1: 10-35, the reaction temperature to be 30-90 ℃, the reaction time to be 1-24 h, carrying out solid-liquid separation after the reaction is finished, and washing the solid residue to be neutral by water; the mixture of the liquid phase obtained after the solid-liquid separation and the washing liquid obtained by washing the solid residue with water is collectively referred to as a lignocellulose alkaline oxidation pretreatment liquid.
Preferably, the lignocellulose alkaline oxidation pretreatment solution is one of bagasse, wheat straw, corn straw or corn cob alkaline oxidation pretreatment solution.
Preferably, the pH of the supernatant obtained in the step (2) is adjusted to 5-6 by acid. The acid is selected from any one of sulfuric acid, hydrochloric acid, acetic acid and citric acid.
Preferably, the pH of the supernatant in the step (3) is adjusted to 4-5 by using an acid, and the acid is selected from any one of sulfuric acid, hydrochloric acid, acetic acid and citric acid.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the method, the solubility difference of lignin, water-insoluble xylan and water-soluble xylan in the lignocellulose alkaline oxidation pretreatment solution in different pH solutions is utilized, the lignocellulose alkaline oxidation pretreatment waste liquid is treated by adopting a gradient adjustment pretreatment solution pH and an enzyme hydrolysis mode, the separation and purification process of lignin and xylan with high organic solvent consumption and high cost is omitted, and the method has the advantages of low treatment cost, green and environment-friendly process and the like.
(2) The method adopts the modes of gradient adjustment of the pH value of the pretreatment solution and enzymatic hydrolysis to treat the lignocellulose alkaline oxidation pretreatment waste liquid, recovers lignin components, coproduces xylo-oligosaccharide and has good industrial application prospect.
The specific implementation mode is as follows:
the following is a further description of the invention and is not intended to be limiting.
Example 1:
carrying out solid-liquid separation on the bagasse alkaline oxidation pretreatment liquid by adopting a centrifugal method to remove solid impurities to obtain a supernatant fluid 1; and (3) regulating the pH value of the supernatant fluid 1 to 5 by using a sulfuric acid solution, concentrating the pretreatment fluid by adopting vacuum rotary evaporation, carrying out solid-liquid separation to obtain a precipitate 1 and a supernatant fluid 2, dispersing the precipitate by using a pH4 buffer solution, adding 50IU/g of endo-xylanase, and hydrolyzing at the reaction temperature of 60 ℃ for 24h to obtain a xylo-oligosaccharide solution. And continuously adjusting the pH value of the supernatant 2 to 4 with acid, performing solid-liquid separation to obtain a precipitate 2 and a supernatant 3, dispersing the precipitate 2 with a pH4 buffer solution, adding 50IU/g of endoxylanase, hydrolyzing at the reaction temperature of 45 ℃ for 24h, and performing solid-liquid separation to obtain a xylo-oligosaccharide solution 2 and lignin 1 respectively. Adding 500IU/mL endo-xylanase into the supernatant 3, hydrolyzing at the reaction temperature of 45 ℃ for 24h, performing solid-liquid separation to obtain a precipitate 3 and a xylo-oligosaccharide solution 3, and washing the precipitate 3 with water to obtain lignin 2. The total yield of xylo-oligosaccharide prepared by the embodiment is 37.57 percent, and the total yield of lignin is 70 percent.
Example 2:
carrying out solid-liquid separation on the alkaline oxidation pretreatment liquid of the corn straws by adopting a centrifugal method to remove solid impurities to obtain a supernatant liquid 1; and (3) regulating the pH value of the supernatant fluid 1 to 5.5 by using a hydrochloric acid solution, concentrating the pretreatment fluid by adopting vacuum rotary evaporation, carrying out solid-liquid separation to obtain a precipitate 1 and a supernatant fluid 2, dispersing the precipitate by using a pH5 buffer solution, adding 500IU/g of endo-xylanase, and hydrolyzing at the reaction temperature of 60 ℃ for 9 hours to obtain a xylo-oligosaccharide solution. And continuously adjusting the pH value of the supernatant 2 to 4 with acid, performing solid-liquid separation to obtain a precipitate 2 and a supernatant 3, dispersing the precipitate 2 with a pH5 buffer solution, adding 500IU/g of endoxylanase, hydrolyzing at the reaction temperature of 60 ℃ for 9 hours, and performing solid-liquid separation to obtain a xylo-oligosaccharide solution 2 and lignin 1 respectively. Adding 50IU/mL endo-xylanase into the supernatant 3, hydrolyzing at the reaction temperature of 45 ℃ for 24h, performing solid-liquid separation to obtain a precipitate 3 and a xylo-oligosaccharide solution 3, and washing the precipitate 3 with water to obtain lignin 2. The total yield of xylo-oligosaccharide prepared by the embodiment is 35.26 percent, and the total yield of lignin is 65 percent.
Example 3:
carrying out solid-liquid separation on the wheat straw alkaline oxidation pretreatment liquid by adopting a centrifugal method to remove solid impurities to obtain a supernatant 1; adjusting the pH value of the supernatant fluid 1 to 6 by using an acetic acid solution, concentrating the pretreatment fluid by adopting vacuum rotary evaporation, carrying out solid-liquid separation to obtain a precipitate 1 and a supernatant fluid 2, dispersing the precipitate by using a pH5 buffer solution, adding 200IU/g of endo-xylanase, and hydrolyzing at the reaction temperature of 45 ℃ for 24h to obtain a xylo-oligosaccharide solution. And continuously adjusting the pH value of the supernatant 2 to 5 with acid, performing solid-liquid separation to obtain a precipitate 2 and a supernatant 3, dispersing the precipitate 2 with a pH5 buffer solution, adding 400IU/g of endoxylanase, hydrolyzing at the reaction temperature of 60 ℃ for 24h, and performing solid-liquid separation to obtain a xylo-oligosaccharide solution 2 and lignin 1 respectively. Adding 400IU/mL endo-xylanase into the supernatant 3, hydrolyzing at the reaction temperature of 60 ℃ for 9h, performing solid-liquid separation to obtain a precipitate 3 and a xylo-oligosaccharide solution 3, and washing the precipitate 3 with water to obtain lignin 2. The total yield of xylo-oligosaccharide prepared by the embodiment is 35.25%, and the yield of lignin is 70%.
Example 4:
carrying out solid-liquid separation on the alkaline oxidation pretreatment liquid of the corncobs by adopting a centrifugal method to remove solid impurities to obtain a supernatant 1; and (3) regulating the pH value of the supernatant fluid 1 to 5 by using a citric acid solution, concentrating the pretreatment fluid by adopting vacuum rotary evaporation, carrying out solid-liquid separation to obtain a precipitate 1 and a supernatant fluid 2, dispersing the precipitate by using a pH5 buffer solution, adding 400IU/g of endo-xylanase, and hydrolyzing at the reaction temperature of 55 ℃ for 12 hours to obtain a xylo-oligosaccharide solution. And continuously adjusting the pH value of the supernatant 2 to 4 with acid, performing solid-liquid separation to obtain a precipitate 2 and a supernatant 3, dispersing the precipitate 2 with a pH5 buffer solution, adding 400IU/g of endoxylanase, hydrolyzing at the reaction temperature of 50 ℃ for 12h, and performing solid-liquid separation to obtain a xylo-oligosaccharide solution 2 and lignin 1 respectively. Adding 400IU/mL endo-xylanase into the supernatant 3, hydrolyzing at the reaction temperature of 60 ℃ for 9h, performing solid-liquid separation to obtain a precipitate 3 and a xylo-oligosaccharide solution 1, and washing the precipitate 3 with water to obtain lignin 2. The total yield of xylo-oligosaccharide prepared by the embodiment is 38.45 percent, and the yield of lignin is 75.22 percent.
Example 5:
carrying out solid-liquid separation on the bagasse alkaline oxidation pretreatment liquid by adopting a centrifugal method to remove solid impurities to obtain a supernatant fluid 1; and (3) regulating the pH value of the supernatant fluid 1 to 6 by using a sulfuric acid solution, concentrating the pretreatment fluid by adopting vacuum rotary evaporation, carrying out solid-liquid separation to obtain a precipitate 1 and a supernatant fluid 2, dispersing the precipitate by using a pH5 buffer solution, adding 300IU/g of endo-xylanase, and hydrolyzing at the reaction temperature of 45 ℃ for 24 hours to obtain a xylo-oligosaccharide solution. And continuously adjusting the pH value of the supernatant 2 to 5 with acid, performing solid-liquid separation to obtain a precipitate 2 and a supernatant 3, dispersing the precipitate 2 with a pH4 buffer solution, adding 300IU/g of endoxylanase, hydrolyzing at the reaction temperature of 60 ℃ for 18h, and performing solid-liquid separation to obtain a xylo-oligosaccharide solution 2 and lignin 1 respectively. Adding 300IU/mL endo-xylanase into the supernatant 3, hydrolyzing at the reaction temperature of 60 ℃ for 9h, performing solid-liquid separation to obtain a precipitate 3 and a xylo-oligosaccharide solution 1, and washing the precipitate 3 with water to obtain lignin 2. The total yield of xylo-oligosaccharide prepared by the embodiment is 31.92 percent, and the yield of lignin is 58.44 percent.
Example 6:
carrying out solid-liquid separation on the wheat straw alkaline oxidation pretreatment liquid by adopting a centrifugal method to remove solid impurities to obtain a supernatant 1; regulating the pH value of the supernatant fluid 1 to 5.5 by using a sulfuric acid solution, concentrating the pretreatment fluid by adopting vacuum rotary evaporation, carrying out solid-liquid separation to obtain a precipitate 1 and a supernatant fluid 2, dispersing the precipitate by using a pH5 buffer solution, adding 500IU/g of endo-xylanase, and hydrolyzing at the reaction temperature of 50 ℃ for 9 hours to obtain a xylo-oligosaccharide solution. And continuously adjusting the pH value of the supernatant 2 to 4.5 by using acid, performing solid-liquid separation to obtain a precipitate 2 and a supernatant 3, dispersing the precipitate 2 by using a pH5 buffer solution, adding 500IU/g of endoxylanase, hydrolyzing at the reaction temperature of 50 ℃ for 9 hours, and performing solid-liquid separation to respectively obtain a xylo-oligosaccharide solution 2 and lignin 1. Adding 50IU/mL endo-xylanase into the supernatant 3, hydrolyzing at the reaction temperature of 60 ℃ for 24h, performing solid-liquid separation to obtain a precipitate 3 and a xylo-oligosaccharide solution 1, and washing the precipitate 3 with water to obtain lignin 2. The total yield of xylo-oligosaccharide prepared by the embodiment is 36.55%, and the total yield of lignin is 68.22%.
The detailed description is specific to possible embodiments of the invention, which are not intended to limit the scope of the invention, but rather are intended to include equivalent implementations or modifications within the scope of the invention.
Claims (4)
1. The method for separating lignin and co-producing xylo-oligosaccharide from the lignocellulose alkaline oxidation pretreatment solution is characterized by comprising the following steps:
(1) performing solid-liquid separation on the lignocellulose alkaline oxidation pretreatment solution by adopting a centrifugal method to obtain supernatant 1; placing the crushed and dried lignocellulose biomass into a glass reactor, adding 0.5-5% by mass of alkali liquor and 0.5-5% by mass of hydrogen peroxide, controlling the solid-liquid mass ratio to be 1: 10-35, the reaction temperature to be 30-90 ℃, the reaction time to be 1-24 h, carrying out solid-liquid separation after the reaction is finished, and washing the solid residues to be neutral by water; the mixture of the liquid phase obtained after the solid-liquid separation and the washing liquid obtained by washing the solid residue with water is collectively called as lignocellulose alkaline oxidation pretreatment liquid;
(2) adjusting the pH value of the supernatant 1 to 5-6, performing vacuum rotary evaporation concentration and solid-liquid separation to obtain a precipitate 1 and a supernatant 2, dispersing the precipitate 1 by using a buffer solution with the pH value of 4-5, adding 50-500 IU/g of endo-xylanase, and hydrolyzing at 45-60 ℃ for 9-24 h to obtain a xylo-oligosaccharide solution 1;
(3) continuously adjusting the pH value of the supernatant 2 obtained in the step (2) to 4-5, performing solid-liquid separation to obtain a precipitate 2 and a supernatant 3, dispersing the precipitate 2 by using a buffer solution with the pH value of 4-5, adding 50-500 IU/g of endo-xylanase, hydrolyzing at 45-60 ℃ for 9-24 h, and performing solid-liquid separation to respectively obtain a xylo-oligosaccharide solution 2 and lignin 1;
(4) adding 50-500 IU/mL of endo-xylanase into the supernatant 3 obtained in the step (3), hydrolyzing at 45-60 ℃ for 9-24 h, performing solid-liquid separation to obtain a precipitate 3 and a xylo-oligosaccharide solution 3, and washing the precipitate 3 with water to obtain lignin 2.
2. The method of claim 1, wherein the lignocellulosic alkaline oxidative pretreatment liquor is derived from one of bagasse, wheat straw, corn stover, or corn cob alkaline oxidative pretreatment liquor.
3. The method according to claim 1 or 2, wherein the pH of the supernatant in the step (2) is adjusted to 5 to 6 by using an acid; the acid is selected from any one of sulfuric acid, hydrochloric acid, acetic acid and citric acid.
4. The method according to claim 1 or 2, wherein the pH of the supernatant in the step (3) is adjusted to 4 to 5 with an acid selected from any one of sulfuric acid, hydrochloric acid, acetic acid and citric acid.
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| CN110016488B (en) * | 2019-04-18 | 2021-03-09 | 中国科学院广州能源研究所 | Method for separating lignin and co-producing xylo-oligosaccharide from lignocellulose alkaline oxidation pretreatment liquid |
| CN112175111B (en) * | 2020-09-28 | 2021-08-10 | 桂林古膳食品科技有限公司 | Method for efficiently separating wood fiber material to obtain high-purity components |
| ES2966032A1 (en) * | 2022-09-19 | 2024-04-17 | Univ Vigo | Production process of products derived from hemicellulose |
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