CN110004093A - A kind of bacillus subtilis culture medium raw material and its preparation method and application, the culture medium for improving bacillomycin D yield - Google Patents
A kind of bacillus subtilis culture medium raw material and its preparation method and application, the culture medium for improving bacillomycin D yield Download PDFInfo
- Publication number
- CN110004093A CN110004093A CN201910332960.0A CN201910332960A CN110004093A CN 110004093 A CN110004093 A CN 110004093A CN 201910332960 A CN201910332960 A CN 201910332960A CN 110004093 A CN110004093 A CN 110004093A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- raw material
- bacillus subtilis
- bacillomycin
- medium raw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The culture medium that the present invention provides a kind of bacillus subtilis culture medium raw material and its preparation method and application, improves bacillomycin D yield, it is related to technical field of microbial fermentation, bacillus subtilis culture medium raw material of the present invention is the enzymatic hydrolysis supernatant of cellulase and zytase double enzymolysis corn stover.The enzymolysis property of cellulose-binding enzyme and zytase of the present invention, using cellulase and zytase double enzymolysis corn bacillus, the enzymatic hydrolysis supernatant that double enzymolysis obtains can effectively improve the yield and combined coefficient of bacillomycin D as culture medium raw material.Bacillomycin D is developed into antiseptics for natural food to realize bacillomycin D large-scale industrialization application, has established scientific and reasonable technical foundation by this.
Description
Technical field
The present invention relates to technical field of microbial fermentation more particularly to a kind of bacillus subtilis culture medium raw material and its systems
Preparation Method and application, a kind of bacillus subtilis bacterium culture medium for improving bacillomycin D yield.
Background technique
China is large agricultural country, and every annual meeting generates a large amount of corn stover, and corn stover contains a large amount of celluloses, hemicellulose
The macromolecular carbohydrates such as element and lignin.If corn stover is directly used in microbial fermentation, it is difficult by microorganism benefit
With.Corn stover is digested, the recuding sugars of more small molecule are obtained, is applied to microbial fermentation, produces some valuable targets
Metabolite has become the common recognition of domestic and international researcher.Nevertheless, the substrate specificity type due to single enzyme is less, enzymatic hydrolysis
Efficiency is lower, so that zymolysis technique is constrained in the application of field of microbial fermentation, and the method for using double enzymolysis, due to effect
Substrate is more, can significantly improve enzymolysis efficiency, the recuding sugars of more polymorphic type small molecule is obtained, to enrich for microbial fermentation
Carbon source.
Bacillomycin D (bacillomyicinD) is a kind of secondary metabolite given birth to by producing bacillus subtilis, is had
Inhibition growth of pathogenic bacteria and antitumor equal physiological activity, have very wide application prospect.Under natural conditions, bacillomycin D
Synthesis capability in bacillus subtilis is extremely limited, and which has limited the industrial applications of bacillomycin D.
Summary of the invention
The present invention provides a kind of bacillus subtilis culture for the defect for overcoming existing bacillomycin D to underproduce
Based raw material and its preparation method and application, the culture medium for improving bacillomycin D yield, using culture medium raw material of the present invention
Culture medium and fermenting B. subtilis are constructed, the yield of bacillomycin D is remarkably improved, to realize that bacillomycin D is extensive
Industrial applications lay the foundation.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of bacillus subtilis culture medium raw material, the culture medium raw material is that cellulase and wood are poly-
The enzymatic hydrolysis supernatant of carbohydrase double enzymolysis corn stover.
Preferably, the mass ratio when cellulase and zytase digest is 1~2:2~3.
Preferably, the Rate activity of the cellulase is 10000~120000U/g, and the Rate activity of the zytase is
10000~120000U/g.
The present invention also provides a kind of preparation method of bacillus subtilis culture medium raw material described in above-mentioned technical proposal, packets
Include following steps:
(1) corn stover, cellulase, zytase and water are mixed, 60~80h are digested under the conditions of 50~60 DEG C,
Obtain enzymatic hydrolysis compound；
(2) the enzymatic hydrolysis compound is separated by solid-liquid separation, obtains the enzymatic hydrolysis supernatant.
Preferably, in the step (1), quality, the quality of cellulase, the quality of zytase and the water of corn stover
The ratio between volume are as follows: 1~2g:1~2g:2~3g:54~74ml.
Preferably, in the step (2), the method for separation of solid and liquid is centrifugation, and the revolving speed of centrifugation is 7000~10000r/
Min, the time of centrifugation are 8~15min.
The present invention also provides bacillus subtilis culture medium raw materials described in preceding solution to improve bacillomycin D production
Application in amount.
The present invention also provides a kind of bacillus subtilis bacterium culture mediums for improving bacillomycin D yield, including aforementioned techniques
Bacillus subtilis culture medium raw material, yeast extract, L-sodium and potassium dihydrogen phosphate described in scheme.
Preferably, in the culture medium, volume, the matter of yeast extract of the bacillus subtilis culture medium raw material
The mass ratio of amount, the quality of L-sodium and potassium dihydrogen phosphate is 100ml:1~4g:5~15g:0.5~2g.
Compared with prior art, beneficial effects of the present invention:
The present invention provides a kind of bacillus subtilis culture medium raw material, the culture medium raw material is that cellulase and wood are poly-
The enzymatic hydrolysis supernatant of carbohydrase double enzymolysis corn stover.The enzymolysis property of cellulose-binding enzyme and zytase of the present invention, utilizes fibre
Tieing up plain enzyme and zytase double enzymolysis corn bacillus, the enzymatic hydrolysis supernatant that double enzymolysis obtains can effectively improve as culture medium raw material
The yield and combined coefficient of bacillomycin D.This develops bacillomycin D to realize bacillomycin D large-scale industrialization application
At antiseptics for natural food, scientific and reasonable technical foundation has been established.
The raw material sources of bacillus subtilis culture medium raw material of the present invention are extensive, at low cost, and preparation method
It is simple and easy to do.The production cost of bacillomycin D can be further decreased, while also improving the added value of corn stover.
Specific embodiment
The present invention provides a kind of bacillus subtilis culture medium raw material, the culture medium raw material is that cellulase and wood are poly-
The enzymatic hydrolysis supernatant of carbohydrase double enzymolysis corn stover.The purpose that the present invention selects cellulase and zytase to carry out double enzymolysis is
Enzymolysis efficiency and reduction sugar type are improved, is conducive to microorganism and utilizes, accumulate thallus, improve the synthesis capability of metabolite.
In the present invention, when double enzymolysis corn stover, the mass ratio of the cellulase and zytase is preferably 1~2:
2~3；More preferably 2:3.
In the present invention, the Rate activity of the cellulase is preferably 10000~120000U/g, more preferably 50000~
100000U/g；The Rate activity of the zytase is preferably 10000~120000U/g, more preferably 50000~100000U/
g。
The present invention also provides the preparation methods of bacillus subtilis culture medium raw material described in above-mentioned technical proposal, including with
Lower step:
(1) corn stover, cellulase, zytase and water are mixed, 60~80h are digested under the conditions of 50~60 DEG C,
Obtain enzymatic hydrolysis compound；
(2) the enzymatic hydrolysis compound is separated by solid-liquid separation, obtains the enzymatic hydrolysis supernatant.
In the present invention, it is 1~2cm that the corn stover is preferably crushed before enzymatic hydrolysis, is crushed to after drying to constant weight
It is more than 60 mesh.In the present invention, the method for the drying is preferably heated-air drying；It is furthermore preferred that the temperature of the heated-air drying
It is 60~70 DEG C, further preferably 65 DEG C.The present invention is sterilizing, enzyme deactivation to the dry purpose to constant weight of corn stover, is convenient for
Preservation.
In the present invention, the temperature of the enzymatic hydrolysis is preferably 50 DEG C.In the present invention, the time of the enzymatic hydrolysis is preferably
70h.In the present invention, the ratio between the quality of the corn stover, the quality of cellulase, the quality of zytase and volume of water
Preferably 1~2g:1~2g:2~3g:54~74ml；More preferably 2g:2g:3g:54ml.
In the present invention, the method for the separation of solid and liquid is preferably centrifugated；The revolving speed of the centrifugation is preferably 7000
~10000r/min, more preferably 8000r/min；The time of the centrifugation is preferably 8~15min, more preferably 10min.
The present invention also provides bacillus subtilis culture medium raw materials described in preceding solution to improve bacillomycin D production
Application in amount.It is fermented using culture medium raw material of the present invention to bacillus subtilis, it is mould to be remarkably improved bacillus
The yield of plain D.As shown in the embodiment of present invention, the enzymatic hydrolysis supernatant digested compared to single enzyme as culture medium raw material,
Bacillomycin D yield significantly increases.
The present invention also provides a kind of bacillus subtilis bacterium culture mediums for improving bacillomycin D yield, including aforementioned techniques
Bacillus subtilis culture medium raw material, yeast extract, L-sodium and potassium dihydrogen phosphate described in scheme.The present invention is preferred
, volume, the quality of yeast extract, the quality of L-sodium and the di(2-ethylhexyl)phosphate of the bacillus subtilis culture medium raw material
The mass ratio of hydrogen potassium is 100ml:1~4g:5~15g:0.5~2g；More preferably 100ml:2g:10g:1g.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
The mature corn stover of no disease and pests harm is selected, dust is removed, cleans up, cut with scissors to 1~2cm segment, put
Enter in electric drying oven with forced convection, temperature is controlled at 65 DEG C, and drying to constant weight.Corn stover segment after taking out drying, sets room temperature
Lower placement after twenty minutes, is crushed with solid high speed disintegrator, crosses 60 meshes, it is spare that powder is made.
The 2.00g maize straw powder for accurately weighing above-mentioned preparation is placed in high-pressure steam sterilizing pan, 121 DEG C of sterilizing 30min
After take out, be cooled to room temperature, into the maize straw powder after sterilizing add cellulase 1g (100000U/g), zytase
2g (100000U/g) and sterile water 54ml, and sufficiently shake up, it is sufficiently digested under the conditions of temperature is 50 DEG C, enzymatic hydrolysis condition
Are as follows: cellulase and zytase addition are than 2:3 (mass ratio, g:g), and enzymolysis time 70h, liquid-to-solid ratio is 27mL/g (enzymolysis liquid
Volume: maize straw powder quality, mL/g).After enzymatic hydrolysis, enzymolysis liquid is taken out, 8000r/min is centrifuged 10min, obtains enzymatic hydrolysis supernatant
Liquid.
Comparative example 1
In addition to not adding cellulase, zytase additive amount is 0.1g, other conditions are same as Example 1.It obtains
Control enzymatic hydrolysis supernatant 1.
Comparative example 2
In addition to not adding zytase, cellulase additive amount is 0.1g, other conditions are same as Example 1.It obtains
Control enzymatic hydrolysis supernatant 2.
Embodiment 2
Each 100ml of enzymatic hydrolysis supernatant of difference Example 1, comparative example 1 and comparative example 2, respectively to each enzymatic hydrolysis supernatant
Middle addition yeast extract 2.0g, L-sodium (monosodium glutamate) 10g and KH2PO41.0g, 115 DEG C of sterilizing 20min, is cooled to room
Bacillomycin D fermentation medium (embodiment 1) and control bacillomycin D fermentation medium 1 (comparative example 1), control bar is made in temperature
Mycomycin D fermentation medium 2 (comparative example 2).
The bacillus subtilis that laboratory is saved, is seeded on slant medium, for 24 hours in 37 DEG C of constant temperature incubations, picking
Single colonie accesses seed culture medium, in 37 DEG C of shaking flask cultures to OD600It is 0.8~1.0, is respectively connected to by 6% inoculum concentration
3 fermentation mediums are stated, in 33 DEG C, 180r/min fermented and cultured 110h.
After fermentation, the fermentation liquid for taking three bacillomycin D fermentation mediums to ferment respectively, each 10.0mL fermentation
Liquid is centrifuged 15min in 6000r/min, abandons thallus, takes supernatant, and supernatant pH is adjusted to 2.0 hereinafter, be stored at room temperature 4h with HCl,
6000r/min is centrifuged 10min, takes precipitating, and 60 DEG C drying to constant weight, and the dry thick peptide solid of bacillomycin D is made.
The dry thick peptide solid of a small amount of bacillomycin D is taken, is ground into a powder, 1.0mL methanol is added and is extracted, 10000r/min
It is centrifuged 10min, takes supernatant, assay, specific HPLC item are carried out to bacillomycin D using high performance liquid chromatography (HPLC) method
The documents such as part and measuring method reference Qian (Shiquan Qian, Hedong Lu, Panpan Meng, Chong Zhang,
Fengxia Lv,Xiaomei Bie,Zhaoxin Lu.Effect of inulin on efficient production
and regulatory biosynthesis of bacillomycin D inBacillus subtilis
FmbJ.Bioresource Technology, 2015,179:260-267) it carries out.It repeats above-mentioned test 5 times, is averaged.
The bacillomycin D content (n=5) that 1 different fermentations culture medium of table ferments
Measurement result is as described in Table 1, it can be seen that, the bacillus fermented using cellulase and zytase double enzymolysis
The yield of bacillomycin D is significantly improved in the thick peptide solid of mycin D.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of bacillus subtilis culture medium raw material, which is characterized in that the culture medium raw material is cellulase and xylan
The enzymatic hydrolysis supernatant of enzyme double enzymolysis corn stover.
2. bacillus subtilis culture medium raw material according to claim 1, which is characterized in that the cellulase and wood are poly-
Mass ratio when carbohydrase digests is 1~2:2~3.
3. bacillus subtilis culture medium raw material according to claim 1 or 2, which is characterized in that the cellulase
Rate activity is 10000~120000U/g, and the Rate activity of the zytase is 10000~120000U/g.
4. the preparation method of bacillus subtilis culture medium raw material described in claims 1 to 3 any one, comprising the following steps:
(1) corn stover, cellulase, zytase and water are mixed, 60~80h is digested under the conditions of 50~60 DEG C, is obtained
Digest compound；
(2) the enzymatic hydrolysis compound is separated by solid-liquid separation, obtains the enzymatic hydrolysis supernatant.
5. the preparation method according to claim 4, which is characterized in that in the step (1), the quality of corn stover, fibre
Tie up the ratio between quality, the quality of zytase and the volume of water of plain enzyme are as follows: 1~2g:1~2g:2~3g:54~74ml.
6. the preparation method according to claim 4, which is characterized in that in the step (2), the method for separation of solid and liquid be from
The heart, the revolving speed of centrifugation are 7000~10000r/min, and the time of centrifugation is 8~15min.
7. bacillus subtilis culture medium raw material described in claims 1 to 3 any one is improving answering in bacillomycin D yield
With.
8. a kind of bacillus subtilis bacterium culture medium for improving bacillomycin D yield, which is characterized in that appoint including claims 1 to 3
Bacillus subtilis culture medium raw material, yeast extract, L-sodium and potassium dihydrogen phosphate described in meaning one.
9. bacillus subtilis bacterium culture medium according to claim 8, which is characterized in that in the culture medium, the withered grass
Volume, the quality of yeast extract, the quality of the quality of L-sodium and potassium dihydrogen phosphate of bacillus culture medium raw material
The ratio between be 100ml:1~4g:5~15g:0.5~2g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910332960.0A CN110004093B (en) | 2019-04-24 | 2019-04-24 | Bacillus subtilis culture medium raw material, preparation method and application thereof, and culture medium for increasing yield of bacillomycin D |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910332960.0A CN110004093B (en) | 2019-04-24 | 2019-04-24 | Bacillus subtilis culture medium raw material, preparation method and application thereof, and culture medium for increasing yield of bacillomycin D |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110004093A true CN110004093A (en) | 2019-07-12 |
| CN110004093B CN110004093B (en) | 2020-09-18 |
Family
ID=67173791
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910332960.0A Active CN110004093B (en) | 2019-04-24 | 2019-04-24 | Bacillus subtilis culture medium raw material, preparation method and application thereof, and culture medium for increasing yield of bacillomycin D |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110004093B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112251374A (en) * | 2020-05-08 | 2021-01-22 | 重庆大学 | High-temperature-resistant high-yield cellulase bacillus subtilis and application thereof |
| CN113528404A (en) * | 2021-08-31 | 2021-10-22 | 淮阴师范学院 | Method for improving yield of bacillomycin D based on segmented fermentation of corn straw enzymatic hydrolysate base material |
| CN114350558A (en) * | 2021-12-31 | 2022-04-15 | 黄河三角洲京博化工研究院有限公司 | Solid oil-removing microbial inoculum and preparation method and application thereof |
| CN114774498A (en) * | 2022-05-06 | 2022-07-22 | 南京财经大学 | Method for producing bacillomycin D by fermenting bacillus immobilized by diatomite |
| CN115466764A (en) * | 2022-09-27 | 2022-12-13 | 淮阴师范学院 | Application of sodium chloride in improvement of bacillus subtilis to synthesis of bacitracin D, sodium chloride fermentation medium and method |
| CN115466765A (en) * | 2022-09-27 | 2022-12-13 | 淮阴师范学院 | Application of magnesium sulfate in improvement of bacillus subtilis to synthesis of bacillomycin D, magnesium sulfate fermentation medium and method |
| CN115478088A (en) * | 2022-09-27 | 2022-12-16 | 淮阴师范学院 | Application of calcium lactate in improvement of bacillus subtilis synthetic bacillomycin D, calcium lactate fermentation culture medium and method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795997A (en) * | 2018-06-11 | 2018-11-13 | 南京理工大学 | The method for producing microbial grease with maize straw acid processing hydrolyzate |
-
2019
- 2019-04-24 CN CN201910332960.0A patent/CN110004093B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795997A (en) * | 2018-06-11 | 2018-11-13 | 南京理工大学 | The method for producing microbial grease with maize straw acid processing hydrolyzate |
Non-Patent Citations (2)
| Title |
|---|
| QIAO, JJ等: "Production of spent mushroom substrate hydrolysates useful for cultivation of Lactococcus lactis by dilute sulfuric acid, cellulase and xylanase treatment", 《BIORESOURCE TECHNOLOGY》 * |
| 张强等: "玉米秸秆的酶法降解机理研究", 《玉米科学》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112251374A (en) * | 2020-05-08 | 2021-01-22 | 重庆大学 | High-temperature-resistant high-yield cellulase bacillus subtilis and application thereof |
| CN113528404A (en) * | 2021-08-31 | 2021-10-22 | 淮阴师范学院 | Method for improving yield of bacillomycin D based on segmented fermentation of corn straw enzymatic hydrolysate base material |
| CN114350558A (en) * | 2021-12-31 | 2022-04-15 | 黄河三角洲京博化工研究院有限公司 | Solid oil-removing microbial inoculum and preparation method and application thereof |
| CN114774498A (en) * | 2022-05-06 | 2022-07-22 | 南京财经大学 | Method for producing bacillomycin D by fermenting bacillus immobilized by diatomite |
| CN114774498B (en) * | 2022-05-06 | 2023-10-03 | 南京财经大学 | Method for producing bacitracin D by fermenting bacillus immobilized by diatomite |
| CN115466764A (en) * | 2022-09-27 | 2022-12-13 | 淮阴师范学院 | Application of sodium chloride in improvement of bacillus subtilis to synthesis of bacitracin D, sodium chloride fermentation medium and method |
| CN115466765A (en) * | 2022-09-27 | 2022-12-13 | 淮阴师范学院 | Application of magnesium sulfate in improvement of bacillus subtilis to synthesis of bacillomycin D, magnesium sulfate fermentation medium and method |
| CN115478088A (en) * | 2022-09-27 | 2022-12-16 | 淮阴师范学院 | Application of calcium lactate in improvement of bacillus subtilis synthetic bacillomycin D, calcium lactate fermentation culture medium and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110004093B (en) | 2020-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110004093A (en) | A kind of bacillus subtilis culture medium raw material and its preparation method and application, the culture medium for improving bacillomycin D yield | |
| Hu et al. | Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing | |
| CN102965311B (en) | Bacillus subtilis and application thereof in preparation of gamma-D-polyglutamic acid | |
| CN103667110B (en) | One bacillus coagulans and use this bacterium synchronous saccharification altogether fermenting lignocellulose to produce the integrated technique of lactic acid | |
| CN106434417A (en) | High-temperature-resistant cellulase producing bacterium and application thereof | |
| WO2018099366A1 (en) | Bacillus subtilis, culture method therefor and application thereof | |
| CN109355227B (en) | Streptomyces violaceus strain and application thereof in cellulose degradation | |
| CN104745643B (en) | A kind of method that ethanol is produced using cigarette stalk fermenting raw materials | |
| Sheng et al. | Direct hydrogen production from lignocellulose by the newly isolated Thermoanaerobacterium thermosaccharolyticum strain DD32 | |
| CN101735993A (en) | Method for efficiently producing cellulase | |
| CN103451133A (en) | Bacillus circulans and application for same in preparation for ferulic acid decarboxylase | |
| CN113528404A (en) | Method for improving yield of bacillomycin D based on segmented fermentation of corn straw enzymatic hydrolysate base material | |
| CN106995790B (en) | Bacterial strain for directly producing butanol by using xylan as unique carbon source and application thereof | |
| CN105734069A (en) | A high-temperature alpha-L-arabinfuranosidease gene, a high-temperature acetylxylan esterase gene, and protein expression and applications of the genes | |
| CN109797121A (en) | The microbial strains LM-1801 of one plant of degraded cellulose and its application | |
| CN106755179A (en) | A kind of culture medium for being suitable to bacteria cellulose fermentation | |
| CN109439585A (en) | One plant of adipic acid kelvin bacterium and its application in cellulose degradation | |
| CN102533570A (en) | Aspergillus niger, application of Aspergillus niger and method for preparing citric acid by fermentation | |
| CN105062928B (en) | A kind of zymomonas mobilis and its application of resisting high-concentration acetic acid and high concentration furtural | |
| Cao et al. | Research on the solid state fermentation of Jerusalem artichoke pomace for producing R, R-2, 3-butanediol by Paenibacillus polymyxa ZJ-9 | |
| TWI719317B (en) | Method for producing lactic acid | |
| CN110283870A (en) | A kind of method of double bacterial strains mixed solid fermentation corn stover | |
| CN103497941B (en) | Method for preparing cellulase through trichoderma viride high-efficiency fermentation | |
| CN104774880A (en) | Preparation method of L-lactic acid by fermenting sweet sorghum straw juice | |
| CN103614418A (en) | Method for producing fuel ethanol through synchronous saccharification and fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |